Rh4CG255900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010154
Physical Location & Seq
Forward (+)
50799188 .. 50806019
6832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG255900.1

Sequence Viewer

Length: 3969 bp
ATGAACTTGCTTAGTGACTCGGTGGAAGCTGTAGCTAGCTGTTCTACTACTGCTTTGGTTTCGTCTCATAATCAAGAGAGATTAGACCTCAAGTCCATTCGGGCGGTTGTCATTTCAAGCAATCAATGCATTCTCAAACTCATAGCAAATGACAAAACTAGAAACTCTATAAGGGTGAGATGTATCTCCAAGCTTCAAAATCAGAAACAGGATTTCTTCGAATTCTCCGACCAGTCTGTCATATCAAATCTTTACTGGGGTATTGACAGCATTGAAGCTGCAATTACCGCCGAATGGCCAGAAGAGAAAGCCGCTCAGCTGAGGAAGGCAGAGCAGATGCTACAAGTGCCCGCGCTACTCGATGAGGATGGAGTCACAGCTGGAATTCCGAACAGTTATTTGGTGTGTTGCTCATACTTTTACCTCTCTGTTGTTCGAAAACTCCATGAGGATGAGTGGCAGGTTGCACTGCATTTCCTACAGGCCGTGCTGGTCTCCCCGAGACTTGTTCAGACAGAATTTGCGCATGACCTTTATGAAAGTCTTTTCCCGACTTGCGGCGGTTCTGAAAGACAAGAAATAAGAGCGAGTAAGAGCTTGGAATCAATTGACAAGGATGAAGCAATAAGGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGCGCGACTTCTCAGGGTCAAGGTGGAGGATACAGAGGCATTTTGTCCCCTGACAAAGAGTCACTATATTCAATACATGGGAAATCCACTAGGTTGGACTGTTCAAATAATATTGAGCATGACCACAGCTTGCACACTCAATGGAATTATGGAAAAGTGCACCCTTTTGATCCTCAAGAAGATATTGGCATCGAAGATGAACTGAAACAATCCATCCACATCTCAGAATTTGAAGAATATGGGAAATCCAAAAATGATTTGAATCAAGCAACAGAGTTTCATGTTAAAACTAGAGAGCTCCAGAAGAATTCAAATATTAAAAGACTCCAAGATTTGCTGAATGACTCTCAATCAGACACACCAACTTCAGTGGATTCATGCTCTGATTATTCAGATTATGACATTGAGTCAGAGGTCATTGATGGTGGTGAGTGCTCAACCAGAGATGCAAGAATAGGTGCAGATTTTTCCAAGAAACTGCAGGCTACTAGCTCCACATCAGACCCAAAGTACGAAGCATTATCTTTTTCACAAGTCTGTCAGGATCCAGTCCCTAACGAAGTCATTCAAGTAAATAACTCTATGATTCTTTCGAGCAGATTCACAAATTCCATCAATGGTTTATTAACTATTTCAGAGCATAGAGATAACAGATCAAAGACCCATCATAATTTATATGTACAAAAGGAGTCTGCATCTCAGCGGAACTATAGGATCAACCAAAGAAGTATTGCAAGGAAAAAACATAGTTCTCGTAGCCAGAAAAGCTCTATTGAACTTCGCTTACATTCCACAAAAGATTCGAAAAGTGAACTACTGAGTATTACTGAAAAAGCAATTTCAAAACTATTTCAGTGGGAAGGATTGGGAAAATGGGATGAAGATTATGCTGTAGAAGTTACAACAATATATCAAATACTATGCAACAAAAAAGGAGAGAAATGTGCTGTCTTGAAAGACATGATATTGGATCAACTGCTCATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTGATAAGGATATCAGTGTCTATACTAACTACCATTGTTGCAGCTAATAAGTCAGTTATCGAGGACATAAAGAAGAAAGGTCTGCAGTTATATGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCATCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTGTTGGGGGTCGTGTGCTCTCCAAACAGTTATAAGGGTAGGCCAGCATCAGTGCCAACACCTCTGACAGCATCACTAATGATTATTGGAGTTTTAGTATCTTCATTTGACCATGCCACAAATAATGTGCACTTGGCTGAAATCAGTTATCCCAATGTGCTGCATGGGCTCCTAGATGTTGCAAGAGATAGTAACATTGCAGAATTGATCTCCTGGGCCACTATTCTTGTCAAATGCATACAATACGATGGAAACTGCAGAAGATATATATCAAGATTAGCTCCAATGGCTCCATTTTCCCGTCTCCTAGAATGTAAGATGAAGCATGCCAGGTGCATAGCGCTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCGGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACGAAGATTATGAACTCACTCATGCTTTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCATTTAGATACACTAGATAACTCCTCCTGTAAGAGTGCATTTACAGAAGAAGCTATGCAGGTTCTCCTCAAGTTGGTCGCATCTGAAGAAAGTTCCACTACACAGCACTTATCTGCATTCATCTTATCAAATCTCGGTGGAACATATTCATGGGCAGGGGAACCATATACAGTAGCATGGTTGGTTAAAAAGGCAGGTGTGACTTCCTCGTATCGACGGAATATGATAAAAACTATCCATTGGTTGGATGATTGCTTAGAGGATTCTGGCACGGACTCATGGTGCAGCAAAATTTCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTGATTCTTTAGTGAAGGGTCTAAAGAGCACAACAAGGAAGGTTTCTAGGGACTGTCTAATAGCCATAGCATGGCTTGGGTTTGAAATTGCAAAGAGTCCAGATATCATCAGATATTCTGCCTGTGAGATCTTACTTAGTGGAGTTGAGCAATTTTTGCATCCTGGATTGGAGCTAGAAGAACGAGTTCTAGCATGTCTCTGCATATATAACTATGCTTCTGGTAAAGGGATGAAAAAGTTAATTCATTTCTCGGAAGGAGTGAGAGAATCACTAAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGATCACGAATTTCTTGTGTTCATACCCAAATTCTCGAGGTTGGAGTCAATTACAGTGGAGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGCTGCTCAGATGGTTCTGTGAAGGTCTGGAACATAAAAGGTCAGTCGGCCACACTCGTATGGGACATGAAGGAGCACAAAAAGGCAGTTACATGCTTTTCACTACTTGAATCCAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTATAAGGGTCTGGCAAGTGGTCCGCAAAAAACTGGAGTGCATTGAGGTGATAGAAACCAAGGAACCAATTCGGCATCTGAATACATGCGGGGACATGATATTTGCAATTACCCGTGGCCATGGAATTAAGGTGATTGATGCATCAAGAAAGGTCAAAGATAATTGCACGAATAAAAAGGTAAAGTGTATGGCGGTGGTTCAGGGGAAGATTTATGCAGGTTGCAAGGATTCAAGCATACAGGAGTTGTCTACAACAAGCAATCGGGCACAAGAGATCAAAGCAGCATCAAAATTTTGGAGGCTGCAACGGAGACCCATAAATGCAGTTGTCACGTATAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGCAAGGTTCAAACTTGAAGGAATGGAAAAGACACGGCAGACCCCAAATGACACTTAAAACCGGGAAAAGAGAAAAGGTGACGGCAATGGGAATAACGGAAGACTTCATCTACCTCAACTGCAACTCATCAACAAACAGCATCCAGATATGGCTAAGAGGGACACAGCAGAAAGTGGGCAGAATCTCAGCAGGAAGCAGAATTACGAGCATCCTCACAGCCAACGACATTATCTTGTGTGGTACCGAAACCGGGCTAATCAAGGGATGGATCCCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1322

Amino Acids

148.35

Weight (kDa)

8.42

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 33 - 184 6.3e-28 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 499 - 660 4.5e-48 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 664 - 1016 8.3e-56 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
WD40_CDC20-Fz PF24807 1044 - 1133 1e-05 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 1046 - 1113 4.3e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 1055 - 1191 3.8e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 1055 - 1132 2.4e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1056 - 1118 4.8e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 1056 - 1130 6.2e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 669, 1926, 3299
AarI CACCTGC 1 cut(s) 2582
AasI GACNNNNNNGTC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 525
Acc36I ACCTGC 4 cut(s) 451, 2446, 2582, 3527
Acc65I GGTACC 1 cut(s) 3929
AccB1I GGYRCC 1 cut(s) 3929
AccB7I CCANNNNNTGG 3 cut(s) 2377, 2641, 2796
AccBSI CCGCTC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 3569
AccII CGCG 2 cut(s) 353, 689
AclWI GGATC 8 cut(s) 848, 1223, 1236, 1406, 1662, 3313, 3952, 3965
AcoI YGGCCR 3 cut(s) 296, 3219, 3436
AcuI CTGAAG 2 cut(s) 1035, 2502
AfaI GTAC 3 cut(s) 1196, 1365, 3931
AfeI AGCGCT 1 cut(s) 2235
AfiI CCNNNNNNNGG 7 cut(s) 294, 557, 2377, 2470, 2641, 2796, 3939
AhdI GACNNNNNGTC 3 cut(s) 91, 742, 1090
AjnI CCWGG 5 cut(s) 2105, 2222, 2887, 3020, 3284
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
Alw21I GWGCWC 8 cut(s) 846, 984, 1121, 1804, 1913, 2025, 2756, 3249
Alw26I GTCTC 6 cut(s) 69, 496, 499, 2201, 2927, 3625
Alw44I GTGCAC 2 cut(s) 842, 2021
AlwI GGATC 8 cut(s) 848, 1223, 1236, 1406, 1662, 3313, 3952, 3965
AlwNI CAGNNNCTG 1 cut(s) 2299
Ama87I CYCGRG 2 cut(s) 499, 3104
Aor51HI AGCGCT 1 cut(s) 2235
AoxI GGCC 6 cut(s) 296, 483, 1934, 2109, 3219, 3436
ApaLI GTGCAC 2 cut(s) 842, 2021
ArsI GACNNNNNNTTYG 2 cut(s) 3404, 3436
Asp700I GAANNNNTTC 5 cut(s) 1248, 2244, 2542, 2910, 3387
Asp718I GGTACC 1 cut(s) 3929
AspLEI GCGC 4 cut(s) 355, 526, 689, 2236
AspS9I GGNCC 2 cut(s) 2109, 3340
AsuC2I CCSGG 2 cut(s) 3751, 3940
AsuHPI GGTGA 5 cut(s) 187, 1124, 3379, 3463, 3778
AsuII TTCGAA 3 cut(s) 219, 436, 1487
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 2 cut(s) 499, 3104
AvaII GGWCC 1 cut(s) 3340
BaeGI GKGCMC 5 cut(s) 351, 846, 2025, 3142, 3589
BalI TGGCCA 2 cut(s) 298, 3438
BamHI GGATCC 2 cut(s) 1228, 3957
BanI GGYRCC 1 cut(s) 3929
BanII GRGCYC 2 cut(s) 984, 2064
BarI GAAGNNNNNNTAC 4 cut(s) 1663, 1695, 3874, 3906
BbsI GAAGAC 1 cut(s) 3795
Bbv12I GWGCWC 8 cut(s) 846, 984, 1121, 1804, 1913, 2025, 2756, 3249
BbvCI CCTCAGC 1 cut(s) 320
BceAI ACGGC 3 cut(s) 470, 3739, 3786
BciT130I CCWGG 5 cut(s) 2107, 2224, 2889, 3022, 3286
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 2 cut(s) 3751, 3940
BcoDI GTCTC 6 cut(s) 69, 496, 499, 2201, 2927, 3625
BfmI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1575, 1778, 1860, 2149, 3965
BfoI RGCGCY 1 cut(s) 2237
BfuAI ACCTGC 4 cut(s) 451, 2446, 2582, 3527
BfuI GTATCC 1 cut(s) 707
BglII AGATCT 1 cut(s) 2853
BlpI GCTNAGC 1 cut(s) 315
Bme1390I CCNGG 7 cut(s) 2107, 2224, 2889, 3022, 3286, 3751, 3940
Bme18I GGWCC 1 cut(s) 3340
BmeRI GACNNNNNGTC 3 cut(s) 91, 742, 1090
BmeT110I CYCGRG 2 cut(s) 499, 3104
BmgT120I GGNCC 2 cut(s) 2109, 3340
BmiI GGNNCC 7 cut(s) 1230, 2063, 2184, 2559, 3384, 3931, 3959
BmrFI CCNGG 7 cut(s) 2107, 2224, 2889, 3022, 3286, 3751, 3940
BmrI ACTGGG 1 cut(s) 265
BmtI GCTAGC 1 cut(s) 39
BmuI ACTGGG 1 cut(s) 265
BoxI GACNNNNGTC 1 cut(s) 3007
BpiI GAAGAC 1 cut(s) 3795
BpmI CTGGAG 2 cut(s) 968, 3374
Bpu10I CCTNAGC 1 cut(s) 320
Bpu1102I GCTNAGC 1 cut(s) 315
Bpu14I TTCGAA 3 cut(s) 219, 436, 1487
BpuEI CTTGAG 3 cut(s) 74, 843, 2450
BpuMI CCSGG 2 cut(s) 3751, 3940
BsaAI YACGTR 1 cut(s) 3654
BsaBI GATNNNNATC 2 cut(s) 945, 2161
BsaI GGTCTC 2 cut(s) 499, 3625
BsaJI CCNNGG 6 cut(s) 2106, 2267, 3285, 3378, 3433, 3439
BsaXI ACNNNNNCTCC 2 cut(s) 1974, 2004
Bsc4I CCNNNNNNNGG 7 cut(s) 294, 557, 2377, 2470, 2641, 2796, 3939
Bse118I RCCGGY 1 cut(s) 1888
Bse1I ACTGG 5 cut(s) 232, 260, 1232, 3315, 3357
Bse3DI GCAATG 2 cut(s) 2088, 3780
Bse8I GATNNNNATC 2 cut(s) 945, 2161
BseBI CCWGG 5 cut(s) 2107, 2224, 2889, 3022, 3286
BseDI CCNNGG 6 cut(s) 2106, 2267, 3285, 3378, 3433, 3439
BseJI GATNNNNATC 2 cut(s) 945, 2161
BseLI CCNNNNNNNGG 7 cut(s) 294, 557, 2377, 2470, 2641, 2796, 3939
BseMI GCAATG 2 cut(s) 2088, 3780
BseMII CTCAG 8 cut(s) 311, 329, 710, 921, 1397, 1493, 3192, 3888
BseNI ACTGG 5 cut(s) 232, 260, 1232, 3315, 3357
BseRI GAGGAG 4 cut(s) 1703, 2410, 2453, 3047
BseSI GKGCMC 5 cut(s) 351, 846, 2025, 3142, 3589
BsgI GTGCAG 2 cut(s) 1164, 2701
Bsh1236I CGCG 2 cut(s) 353, 689
BshFI GGCC 6 cut(s) 298, 485, 1936, 2111, 3221, 3438
BshNI GGYRCC 1 cut(s) 3929
BsiHKAI GWGCWC 8 cut(s) 846, 984, 1121, 1804, 1913, 2025, 2756, 3249
BsiHKCI CYCGRG 2 cut(s) 499, 3104
BsiSI CCGG 3 cut(s) 1889, 3750, 3939
BslFI GGGAC 7 cut(s) 715, 1220, 2789, 3248, 3302, 3425, 3862
BslI CCNNNNNNNGG 7 cut(s) 294, 557, 2377, 2470, 2641, 2796, 3939
BsmAI GTCTC 6 cut(s) 69, 496, 499, 2201, 2927, 3625
BsmBI CGTCTC 2 cut(s) 69, 2201
BsmFI GGGAC 7 cut(s) 715, 1220, 2789, 3248, 3302, 3425, 3862
BsmI GAATGC 3 cut(s) 129, 2262, 2513
BsnI GGCC 6 cut(s) 298, 485, 1936, 2111, 3221, 3438
Bso31I GGTCTC 2 cut(s) 499, 3625
BsoBI CYCGRG 2 cut(s) 499, 3104
Bsp119I TTCGAA 3 cut(s) 219, 436, 1487
Bsp1407I TGTACA 1 cut(s) 1363
Bsp1720I GCTNAGC 1 cut(s) 315
Bsp19I CCATGG 1 cut(s) 3439
BspANI GGCC 6 cut(s) 298, 485, 1936, 2111, 3221, 3438
BspCNI CTCAG 8 cut(s) 312, 328, 709, 920, 1396, 1494, 3191, 3887
BspFNI CGCG 2 cut(s) 353, 689
BspHI TCATGA 2 cut(s) 1819, 2248
BspLI GGNNCC 7 cut(s) 1230, 2063, 2184, 2559, 3384, 3931, 3959
BspMAI CTGCAG 3 cut(s) 1167, 1782, 2153
BspMI ACCTGC 4 cut(s) 451, 2446, 2582, 3527
BspOI GCTAGC 1 cut(s) 39
BspPI GGATC 8 cut(s) 848, 1223, 1236, 1406, 1662, 3313, 3952, 3965
BspT104I TTCGAA 3 cut(s) 219, 436, 1487
BspT107I GGYRCC 1 cut(s) 3929
BspTNI GGTCTC 2 cut(s) 499, 3625
BsrBI CCGCTC 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 2088, 3780
BsrFI RCCGGY 1 cut(s) 1888
BsrGI TGTACA 1 cut(s) 1363
BsrI ACTGG 5 cut(s) 232, 260, 1232, 3315, 3357
BssAI RCCGGY 1 cut(s) 1888
BssECI CCNNGG 6 cut(s) 2106, 2267, 3285, 3378, 3433, 3439
BssT1I CCWWGG 3 cut(s) 2267, 3378, 3439
Bst2UI CCWGG 5 cut(s) 2107, 2224, 2889, 3022, 3286
Bst4CI ACNGT 8 cut(s) 395, 785, 1836, 1922, 2569, 2780, 3125, 3674
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 3 cut(s) 126, 2299, 2881
BstAUI TGTACA 1 cut(s) 1363
BstBAI YACGTR 1 cut(s) 3654
BstBI TTCGAA 3 cut(s) 219, 436, 1487
BstC8I GCNNGC 6 cut(s) 37, 351, 815, 1167, 1938, 2220
BstDSI CCRYGG 2 cut(s) 3433, 3439
BstFNI CGCG 2 cut(s) 353, 689
BstH2I RGCGCY 1 cut(s) 2237
BstHHI GCGC 4 cut(s) 355, 526, 689, 2236
BstMAI GTCTC 6 cut(s) 69, 496, 499, 2201, 2927, 3625
BstMWI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1449, 2059, 2180, 2299, 2881, 3137
BstNI CCWGG 5 cut(s) 2107, 2224, 2889, 3022, 3286
BstNSI RCATGY 4 cut(s) 2222, 2922, 3267, 3408
BstPAI GACNNNNGTC 1 cut(s) 3007
BstSCI CCNGG 7 cut(s) 2105, 2222, 2887, 3020, 3284, 3749, 3938
BstSFI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1575, 1778, 1860, 2149, 3965
BstSLI GKGCMC 5 cut(s) 351, 846, 2025, 3142, 3589
BstUI CGCG 2 cut(s) 353, 689
BstV2I GAAGAC 1 cut(s) 3795
BstX2I RGATCY 3 cut(s) 1228, 2853, 3957
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 3 cut(s) 1228, 2853, 3957
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 6 cut(s) 298, 485, 1936, 2111, 3221, 3438
BtgI CCRYGG 2 cut(s) 3433, 3439
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 8 cut(s) 467, 1059, 1544, 1716, 1950, 3130, 3308, 3679
BveI ACCTGC 4 cut(s) 451, 2446, 2582, 3527
Cac8I GCNNGC 6 cut(s) 37, 351, 815, 1167, 1938, 2220
CaiI CAGNNNCTG 1 cut(s) 2299
CciI TCATGA 2 cut(s) 1819, 2248
CfoI GCGC 4 cut(s) 355, 526, 689, 2236
Cfr10I RCCGGY 1 cut(s) 1888
Cfr13I GGNCC 2 cut(s) 2109, 3340
CseI GACGC 1 cut(s) 3012
Csp6I GTAC 3 cut(s) 1195, 1364, 3930
CspCI CAANNNNNGTGG 6 cut(s) 1035, 1070, 2101, 2136, 3106, 3141
CviQI GTAC 3 cut(s) 1195, 1364, 3930
DrdI GACNNNNNNGTC 1 cut(s) 236
DriI GACNNNNNGTC 3 cut(s) 91, 742, 1090
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 3 cut(s) 296, 3219, 3436
Eam1104I CTCTTC 1 cut(s) 297
Eam1105I GACNNNNNGTC 3 cut(s) 91, 742, 1090
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 982
Eco130I CCWWGG 3 cut(s) 2267, 3378, 3439
Eco24I GRGCYC 2 cut(s) 984, 2064
Eco31I GGTCTC 2 cut(s) 499, 3625
Eco32I GATATC 2 cut(s) 1707, 2830
Eco47I GGWCC 1 cut(s) 3340
Eco47III AGCGCT 1 cut(s) 2235
Eco53kI GAGCTC 1 cut(s) 982
Eco57I CTGAAG 2 cut(s) 1035, 2502
Eco88I CYCGRG 2 cut(s) 499, 3104
EcoICRI GAGCTC 1 cut(s) 982
EcoRI GAATTC 3 cut(s) 221, 384, 991
EcoRII CCWGG 5 cut(s) 2105, 2222, 2887, 3020, 3284
EcoRV GATATC 2 cut(s) 1707, 2830
EcoT14I CCWWGG 3 cut(s) 2267, 3378, 3439
EcoT22I ATGCAT 3 cut(s) 131, 2132, 3463
EcoT38I GRGCYC 2 cut(s) 984, 2064
ErhI CCWWGG 3 cut(s) 2267, 3378, 3439
Esp3I CGTCTC 2 cut(s) 69, 2201
FalI AAGNNNNNCTT 4 cut(s) 3147, 3179, 3651, 3683
FaqI GGGAC 7 cut(s) 715, 1220, 2789, 3248, 3302, 3425, 3862
FauI CCCGC 2 cut(s) 358, 3401
FblI GTMKAC 1 cut(s) 3569
FriOI GRGCYC 2 cut(s) 984, 2064
FspI TGCGCA 1 cut(s) 525
GlaI GCGC 4 cut(s) 354, 525, 688, 2235
GsuI CTGGAG 2 cut(s) 968, 3374
HaeII RGCGCY 1 cut(s) 2237
HaeIII GGCC 6 cut(s) 298, 485, 1936, 2111, 3221, 3438
HapII CCGG 3 cut(s) 1889, 3750, 3939
HgaI GACGC 1 cut(s) 3012
HhaI GCGC 4 cut(s) 355, 526, 689, 2236
Hin6I GCGC 4 cut(s) 353, 524, 687, 2234
HinP1I GCGC 4 cut(s) 353, 524, 687, 2234
HindIII AAGCTT 1 cut(s) 191
HpaII CCGG 3 cut(s) 1889, 3750, 3939
HphI GGTGA 5 cut(s) 187, 1124, 3379, 3463, 3778
Hpy166II GTNNAC 4 cut(s) 844, 1496, 2023, 3570
Hpy8I GTNNAC 4 cut(s) 844, 1496, 2023, 3570
Hpy99I CGWCG 1 cut(s) 2616
HpyAV CCTTC 9 cut(s) 319, 1538, 2303, 2734, 2758, 2975, 3187, 3235, 3700
HpyCH4III ACNGT 8 cut(s) 395, 785, 1836, 1922, 2569, 2780, 3125, 3674
HpyCH4IV ACGT 1 cut(s) 3653
HpyF10VI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1449, 2059, 2180, 2299, 2881, 3137
HpySE526I ACGT 1 cut(s) 3653
HspAI GCGC 4 cut(s) 353, 524, 687, 2234
KpnI GGTACC 1 cut(s) 3933
LmnI GCTCC 9 cut(s) 987, 1181, 2067, 2179, 2188, 2896, 3034, 3128, 3244
MaeII ACGT 1 cut(s) 3653
MbiI CCGCTC 1 cut(s) 314
MfeI CAATTG 3 cut(s) 606, 2354, 3684
MflI RGATCY 3 cut(s) 1228, 2853, 3957
MlsI TGGCCA 2 cut(s) 298, 3438
MluNI TGGCCA 2 cut(s) 298, 3438
MmeI TCCRAC 5 cut(s) 252, 759, 2374, 2622, 3091
Mox20I TGGCCA 2 cut(s) 298, 3438
Mph1103I ATGCAT 3 cut(s) 131, 2132, 3463
MroXI GAANNNNTTC 5 cut(s) 1248, 2244, 2542, 2910, 3387
MscI TGGCCA 2 cut(s) 298, 3438
MseI TTAA 9 cut(s) 969, 1002, 1310, 1869, 2583, 2706, 2966, 3447, 3744
MslI CAYNNNNRTG 4 cut(s) 450, 2545, 3229, 3365
Msp20I TGGCCA 2 cut(s) 298, 3438
MspA1I CMGCKG 3 cut(s) 319, 380, 1387
MspI CCGG 3 cut(s) 1889, 3750, 3939
MspR9I CCNGG 7 cut(s) 2107, 2224, 2889, 3022, 3286, 3751, 3940
MunI CAATTG 3 cut(s) 606, 2354, 3684
Mva1269I GAATGC 3 cut(s) 129, 2262, 2513
MvaI CCWGG 5 cut(s) 2107, 2224, 2889, 3022, 3286
MvnI CGCG 2 cut(s) 353, 689
MwoI GCNNNNNNNGC 9 cut(s) 126, 287, 346, 1449, 2059, 2180, 2299, 2881, 3137
NciI CCSGG 2 cut(s) 3751, 3940
NcoI CCATGG 1 cut(s) 3439
NheI GCTAGC 1 cut(s) 35
NlaIV GGNNCC 7 cut(s) 1230, 2063, 2184, 2559, 3384, 3931, 3959
NmuCI GTSAC 6 cut(s) 14, 373, 744, 2596, 3649, 3766
NsbI TGCGCA 1 cut(s) 525
NsiI ATGCAT 3 cut(s) 131, 2132, 3463
NspI RCATGY 4 cut(s) 2222, 2922, 3267, 3408
NspV TTCGAA 3 cut(s) 219, 436, 1487
PaeI GCATGC 1 cut(s) 2222
PaeR7I CTCGAG 1 cut(s) 3104
PagI TCATGA 2 cut(s) 1819, 2248
PaqCI CACCTGC 1 cut(s) 2582
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 3 cut(s) 129, 2262, 2513
PdmI GAANNNNTTC 5 cut(s) 1248, 2244, 2542, 2910, 3387
PflMI CCANNNNNTGG 3 cut(s) 2377, 2641, 2796
PfoI TCCNGGA 1 cut(s) 2887
Ppu21I YACGTR 1 cut(s) 3654
PshAI GACNNNNGTC 1 cut(s) 3007
PsiI TTATAA 3 cut(s) 669, 1926, 3299
Psp124BI GAGCTC 1 cut(s) 984
Psp6I CCWGG 5 cut(s) 2105, 2222, 2887, 3020, 3284
PspGI CCWGG 5 cut(s) 2105, 2222, 2887, 3020, 3284
PspN4I GGNNCC 7 cut(s) 1230, 2063, 2184, 2559, 3384, 3931, 3959
PspPI GGNCC 2 cut(s) 2109, 3340
PsrI GAACNNNNNNTAC 2 cut(s) 1452, 1484
PstI CTGCAG 3 cut(s) 1167, 1782, 2153
PstNI CAGNNNCTG 1 cut(s) 2299
PsuI RGATCY 3 cut(s) 1228, 2853, 3957
PvuII CAGCTG 2 cut(s) 319, 380
RsaI GTAC 3 cut(s) 1196, 1365, 3931
RsaNI GTAC 3 cut(s) 1195, 1364, 3930
RseI CAYNNNNRTG 4 cut(s) 450, 2545, 3229, 3365
SacI GAGCTC 1 cut(s) 984
SaqAI TTAA 9 cut(s) 969, 1002, 1310, 1869, 2583, 2706, 2966, 3447, 3744
Sau96I GGNCC 2 cut(s) 2109, 3340
ScrFI CCNGG 7 cut(s) 2107, 2224, 2889, 3022, 3286, 3751, 3940
SfcI CTRYAG 9 cut(s) 30, 479, 1163, 1393, 1575, 1778, 1860, 2149, 3965
Sfr274I CTCGAG 1 cut(s) 3104
SfuI TTCGAA 3 cut(s) 219, 436, 1487
SinI GGWCC 1 cut(s) 3340
SlaI CTCGAG 1 cut(s) 3104
SmiMI CAYNNNNRTG 4 cut(s) 450, 2545, 3229, 3365
SmlI CTYRAG 4 cut(s) 89, 858, 2465, 3104
SmoI CTYRAG 4 cut(s) 89, 858, 2465, 3104
SphI GCATGC 1 cut(s) 2222
SspI AATATT 2 cut(s) 796, 1000
SstI GAGCTC 1 cut(s) 984
StyD4I CCNGG 7 cut(s) 2105, 2222, 2887, 3020, 3284, 3749, 3938
StyI CCWWGG 3 cut(s) 2267, 3378, 3439
TaaI ACNGT 8 cut(s) 395, 785, 1836, 1922, 2569, 2780, 3125, 3674
TaiI ACGT 1 cut(s) 3656
TaqI TCGA 9 cut(s) 219, 360, 436, 876, 1277, 1487, 1755, 2611, 3105
TatI WGTACW 1 cut(s) 1363
TauI GCSGC 2 cut(s) 314, 561
Tru1I TTAA 9 cut(s) 969, 1002, 1310, 1869, 2583, 2706, 2966, 3447, 3744
Tru9I TTAA 9 cut(s) 969, 1002, 1310, 1869, 2583, 2706, 2966, 3447, 3744
TscAI CASTG 8 cut(s) 474, 1059, 1544, 1716, 1950, 3130, 3315, 3679
TseFI GTSAC 6 cut(s) 14, 373, 744, 2596, 3649, 3766
Tsp45I GTSAC 6 cut(s) 14, 373, 744, 2596, 3649, 3766
TspGWI ACGGA 4 cut(s) 2629, 2684, 3643, 3800
TspRI CASTG 8 cut(s) 474, 1059, 1544, 1716, 1950, 3130, 3315, 3679
Van91I CCANNNNNTGG 3 cut(s) 2377, 2641, 2796
VneI GTGCAC 2 cut(s) 842, 2021
VpaK11BI GGWCC 1 cut(s) 3340
XceI RCATGY 4 cut(s) 2222, 2922, 3267, 3408
XhoI CTCGAG 1 cut(s) 3104
XmiI GTMKAC 1 cut(s) 3569
XmnI GAANNNNTTC 5 cut(s) 1248, 2244, 2542, 2910, 3387
Zsp2I ATGCAT 3 cut(s) 131, 2132, 3463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.