Rw4G020840

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010154
Physical Location & Seq
Forward (+)
44953141 .. 44959629
6489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020840.1

Sequence Viewer

Length: 3621 bp
ATGCTTCAAGTGCCCGCGCTACTCGATGAGGATGGAGTCACAGCTGGAATTCCGAACAGTTATTTGGTGTGTTGCTCATACTTTTACCTCTCTGTTGTTCGAAAACTCCAAGATGATGAGTGGCAGGTTGCACTGCATTTCCTACAGGCCGTGCTGGTCTCCCCGAGACTTGTTCAGACAGAATTTGCGTATGACCTTTACGAAAGTCTTTTCCCGACTTGCGGCGGTTCTGAAAGACAAGAAGAAATAAGAGCGAGTAAGAGCTTGGAATCAATTGACAAGGATGAAGCAATAAGGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGCGCGACTTCTCAGGGTCAAGGTGGAGGATACAGAGGCATTTTGTCCCCTGACAAAGAGTCACTATATTCAATACATGGGAAATCCACTAGGTCGGACTGTTCAAATAATATTGAGCATGACCACAGCTTGCACACTCAATGGAATTATGGAAAAGTGCACCCTTTTGATCCTCAAGAAGATATTGGCATCTCAGAATTTGAAGAATATGGGAAATCCAAAAATGATTTGAATCAAGCAACAGAGTTTCAAGTTCAAACTACAGAGCTCCAGAGGAATTCAAGTATTAAAAGACTCCAAGATTTGCTGAATGACTCTCAATCAGACACACCAACTTCAGTGGATTCATGCTCTGATTATTCAGATTATGACATTGAGTCAGAGGTCATTGATGGTGGTGAGTGCTCAACCAGAGATGCAAGAATAGGTGCAGATTTTTCCAAGAAACTGCAGGCTAGTAGCTCCACATCAGACCCAAAGTACGAAGCATTATCTTTTTCACGAGTCTGTCAGGATCCAGTCCCTAACGAAGTCAGTCAAGTAAATAACTCTATGATTCTTTCGAGCAGATTCACAAATTCCATCAATGGTTTATTAACTATTTCAGAGCATAGAGATAAAAGATCAAAGACCCATCAGAATTTATATGTACAAAAGGAGTATGCATCTCAGCGGAACTATAGAATCAACCAAAGGGATCACCAAAGAAGTGTTGCAAGGAAAAAACATAGTTCTCGTAGCCAGAAAAGCTCTATTGAACTTCGCTTACATTCCACAAAAGATTCGAAAAGTGAACTACTGAGTATTACTGAAAAAGCAATTTCAAAACTATTTCAGTGGGAAGGATTGGGAAAATGGGATGAAGATTATGCTGTAGAAGTTACAACAATATATCAAATACTATGCAACAAAAAAGGAGAGAAATGTGCTGTCTTGAAAGACATGATATTGGATCAACTGCTCATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTGATAAGGGTATCAGTGTCTATACTAACTACTATTGTTGCAGCTAATAAGTCAGTTATCGAGGACATAAAGAAGAAAGGTCTGCAGTTATCTGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCATCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTGTTGGGGGTCGTGTGCTCTCCAAACAGTTATAGGGGTAGGCCAGCATCAGTGCCAACACCTCTGACAGCATCACTAATGATTATTGGAGTTTTAGTATCTTCATTTGACCATGCCACAAATAATGTGCACTTGGCTGAAATCAGTTATCCCAATGTGCTGCATGGGCTCCTAGATGTTGCAAGAGAGAGTAACATTGAAGAATTGATTTCCTGGGCCACTATTCTTGTCAAATGCATACAATACGATGGAAACTGCAGAAGATATATATCAAGATTAGCTCCAATGGCTCCATTTTCCCGTCTCCTAGAATGTAAGATGAAGCATGCCAGGTGCATAGCGCTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCGGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACGAAGATTATGAACTCACTCATGCTTTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCATTTAGATACACTAGATAACTCCTCCTGTAAGAGTGCATTTACAGAAGAAGCTATGCAGGTTCTCCTCAAGTTGGTCGCATCTGAAGAAAGTTCCACTACACAGCACTTATCTGCATTCATCTTATCAAATCTCGGTGGAACATATTCCTGGGCAGGGGAACCATATACAGTAGCATGGTTGGTTAAAAAAGCAGGTGTGACTTCCTCGTATCGACGGAATATGATAAAAACTATCCATTGGTTGGATGATTGCTTAGAGGATTCTGGCACGGACTCATGGTGCAGCAAAATTTCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTGATTCTTTAGTGAAGGGTCTAAAGAGCACAACAAGGAAGGTTTCTAGGGACTGTCTAATAGCCATAGCATGGCTTGGGTTTGAAATTGCAAAGAGTCCAGATATCATCAGATATTCTGCCTGTGAGATCTTACTTAGTGGAGTAGAGCAATTTTTGCATCCTGGATTGGAGCTAGAAGAACGAGTTCTAGCATGTCTCTGCATATATAACTATGCTTCTGGTAAAGGGATGAAAAAGTTAATTCAGTTCTCGGAAGGAGTGAGAGAATCACTAAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGATCACAGCGAATTTCTTGTGTTCATACCCAAATTCTCGAGGTTGGAGTCCATTACAGTGGAGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGCTACTCAGATGGTTCTGTGAAGGTCTGGAACATAAAAGGTCAGTCAGCCACACTCGTATGGGACATGAAGGAGCACAAAAAGGCAGTTACATGCTTTTCACTACTTGAATCCAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTATAAGGGTCTGGCAAGTGGTCCGCAAAAAACTGGAGTGCATTGAGGTGATAGAAACCAAGGATCCAATTCGGCATCTGAATACATGCGGGGACATGATATTTGCAATTACCCGTGGCCATGGAATTAAGGTGATTGATGCATCAAGAAAGGTCAAAGATAATTGCACGAATAAAAAGGTAAAGTGTATGGCGGTGGTTCAGGGGAAGATTTATGTAGGTTGCAAGGATTCAAGCATACAGGAGTTGTCTACAACAAGCAATCGGGCACAAGAGATCAAAGCAGCATCAAAATTTTGGAGGCTGCAACGGAGACCCATAAATGCAGTTGTCACGTATAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGCAAGGTTCAAACTTGAAGGAATGGAAAAGACACGGCAAACCCCAAATGTCACTTAAAACCGGGAAAAGAGAAAAGGTGACGGCAATGGGAATAACGGAAGACTTCATCTACCTCAACTGCAACTCATCAACAAACAGCATCCAGATATGGCTAAGAGGGACACAGCAGAAAGTGGGCAGAATCTCAGCAGGAAGCAGAATTACGAGCATCCTCACAGCCAACGACATTATCTTGTGTGGTACCGAAACCGGGCTAATCAAGGGATGGATCCCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1206

Amino Acids

135.43

Weight (kDa)

8.51

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 9 - 72 4.6e-12 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 382 - 543 7.5e-48 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 547 - 899 1.9e-56 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_TEP1_2nd PF25047 930 - 997 6.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 939 - 1018 4.3e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 941 - 1179 2e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 942 - 1024 3.4e-09 WDR5 beta-propeller domain
WD40_Prp19 PF24814 942 - 1012 1.9e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 336, 2951
AarI CACCTGC 1 cut(s) 2231
Acc36I ACCTGC 3 cut(s) 115, 2095, 2231
Acc65I GGTACC 1 cut(s) 3581
AccB1I GGYRCC 1 cut(s) 3581
AccB7I CCANNNNNTGG 3 cut(s) 2026, 2290, 2445
AccI GTMKAC 1 cut(s) 3221
AccII CGCG 2 cut(s) 17, 356
AciI CCGC 7 cut(s) 15, 222, 225, 1024, 2995, 3060, 3164
AcoI YGGCCR 1 cut(s) 3088
AcuI CTGAAG 2 cut(s) 672, 2151
AfaI GTAC 3 cut(s) 833, 1002, 3583
AfeI AGCGCT 1 cut(s) 1884
AfiI CCNNNNNNNGG 7 cut(s) 221, 2026, 2119, 2202, 2290, 2445, 3591
AhdI GACNNNNNGTC 2 cut(s) 409, 727
AjnI CCWGG 6 cut(s) 1754, 1871, 2195, 2536, 2669, 2936
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
Alw21I GWGCWC 8 cut(s) 513, 621, 758, 1453, 1562, 1674, 2405, 2901
Alw26I GTCTC 5 cut(s) 160, 163, 1850, 2576, 3277
Alw44I GTGCAC 2 cut(s) 509, 1670
AlwNI CAGNNNCTG 1 cut(s) 1948
Ama87I CYCGRG 2 cut(s) 163, 2756
Aor51HI AGCGCT 1 cut(s) 1884
AoxI GGCC 4 cut(s) 147, 1583, 1758, 3088
ApaLI GTGCAC 2 cut(s) 509, 1670
ApeKI GCWGC 9 cut(s) 1385, 1475, 1702, 1948, 2030, 2041, 2331, 3254, 3274
ArsI GACNNNNNNTTYG 2 cut(s) 3056, 3088
Asp700I GAANNNNTTC 3 cut(s) 1893, 2191, 2559
Asp718I GGTACC 1 cut(s) 3581
AspLEI GCGC 3 cut(s) 19, 356, 1885
AspS9I GGNCC 2 cut(s) 1758, 2992
AsuC2I CCSGG 2 cut(s) 3403, 3592
AsuHPI GGTGA 5 cut(s) 761, 1043, 3031, 3115, 3430
AsuII TTCGAA 2 cut(s) 100, 1136
AvaI CYCGRG 2 cut(s) 163, 2756
AvaII GGWCC 1 cut(s) 2992
BaeGI GKGCMC 5 cut(s) 15, 513, 1674, 2794, 3241
BalI TGGCCA 1 cut(s) 3090
BamHI GGATCC 3 cut(s) 865, 3034, 3609
BanI GGYRCC 1 cut(s) 3581
BanII GRGCYC 2 cut(s) 621, 1713
BarI GAAGNNNNNNTAC 4 cut(s) 1312, 1344, 3526, 3558
BauI CACGAG 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 3447
Bbv12I GWGCWC 8 cut(s) 513, 621, 758, 1453, 1562, 1674, 2405, 2901
BbvI GCAGC 9 cut(s) 1397, 1487, 1689, 1935, 2028, 2042, 2343, 3261, 3266
BceAI ACGGC 3 cut(s) 134, 3391, 3438
BciT130I CCWGG 6 cut(s) 1756, 1873, 2197, 2538, 2671, 2938
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 2 cut(s) 3403, 3592
BcoDI GTCTC 5 cut(s) 160, 163, 1850, 2576, 3277
BfaI CTAG 8 cut(s) 441, 807, 1715, 1850, 2060, 2421, 2549, 2564
BfmI CTRYAG 9 cut(s) 143, 612, 800, 1030, 1224, 1427, 1509, 1798, 3617
BfoI RGCGCY 1 cut(s) 1886
BfuAI ACCTGC 3 cut(s) 115, 2095, 2231
BfuI GTATCC 1 cut(s) 374
BglII AGATCT 1 cut(s) 2502
Bme1390I CCNGG 8 cut(s) 1756, 1873, 2197, 2538, 2671, 2938, 3403, 3592
Bme18I GGWCC 1 cut(s) 2992
BmeRI GACNNNNNGTC 2 cut(s) 409, 727
BmeT110I CYCGRG 2 cut(s) 163, 2756
BmgT120I GGNCC 2 cut(s) 1758, 2992
BmiI GGNNCC 7 cut(s) 867, 1712, 1833, 2208, 3036, 3583, 3611
BmrFI CCNGG 8 cut(s) 1756, 1873, 2197, 2538, 2671, 2938, 3403, 3592
BoxI GACNNNNGTC 1 cut(s) 2656
BpiI GAAGAC 1 cut(s) 3447
BpmI CTGGAG 2 cut(s) 605, 3026
Bpu14I TTCGAA 2 cut(s) 100, 1136
BpuEI CTTGAG 2 cut(s) 510, 2099
BpuMI CCSGG 2 cut(s) 3403, 3592
BsaAI YACGTR 1 cut(s) 3306
BsaBI GATNNNNATC 2 cut(s) 582, 1810
BsaI GGTCTC 2 cut(s) 163, 3277
BsaJI CCNNGG 7 cut(s) 1755, 1916, 2196, 2937, 3030, 3085, 3091
BsaXI ACNNNNNCTCC 2 cut(s) 1623, 1653
Bsc4I CCNNNNNNNGG 7 cut(s) 221, 2026, 2119, 2202, 2290, 2445, 3591
Bse118I RCCGGY 1 cut(s) 1537
Bse1I ACTGG 3 cut(s) 869, 2967, 3009
Bse3DI GCAATG 1 cut(s) 3432
Bse8I GATNNNNATC 2 cut(s) 582, 1810
BseBI CCWGG 6 cut(s) 1756, 1873, 2197, 2538, 2671, 2938
BseDI CCNNGG 7 cut(s) 1755, 1916, 2196, 2937, 3030, 3085, 3091
BseJI GATNNNNATC 2 cut(s) 582, 1810
BseLI CCNNNNNNNGG 7 cut(s) 221, 2026, 2119, 2202, 2290, 2445, 3591
BseMI GCAATG 1 cut(s) 3432
BseMII CTCAG 6 cut(s) 377, 558, 1034, 1142, 2844, 3540
BseNI ACTGG 3 cut(s) 869, 2967, 3009
BseRI GAGGAG 4 cut(s) 1352, 2059, 2102, 2696
BseSI GKGCMC 5 cut(s) 15, 513, 1674, 2794, 3241
BseXI GCAGC 9 cut(s) 1397, 1487, 1689, 1935, 2028, 2042, 2343, 3261, 3266
BsgI GTGCAG 2 cut(s) 801, 2350
Bsh1236I CGCG 2 cut(s) 17, 356
BshFI GGCC 4 cut(s) 149, 1585, 1760, 3090
BshNI GGYRCC 1 cut(s) 3581
BsiHKAI GWGCWC 8 cut(s) 513, 621, 758, 1453, 1562, 1674, 2405, 2901
BsiHKCI CYCGRG 2 cut(s) 163, 2756
BsiSI CCGG 3 cut(s) 1538, 3402, 3591
BslFI GGGAC 7 cut(s) 382, 857, 2438, 2900, 2954, 3077, 3514
BslI CCNNNNNNNGG 7 cut(s) 221, 2026, 2119, 2202, 2290, 2445, 3591
BsmAI GTCTC 5 cut(s) 160, 163, 1850, 2576, 3277
BsmBI CGTCTC 1 cut(s) 1850
BsmFI GGGAC 7 cut(s) 382, 857, 2438, 2900, 2954, 3077, 3514
BsmI GAATGC 2 cut(s) 1911, 2162
BsnI GGCC 4 cut(s) 149, 1585, 1760, 3090
Bso31I GGTCTC 2 cut(s) 163, 3277
BsoBI CYCGRG 2 cut(s) 163, 2756
Bsp119I TTCGAA 2 cut(s) 100, 1136
Bsp1407I TGTACA 1 cut(s) 1000
Bsp19I CCATGG 1 cut(s) 3091
BspACI CCGC 7 cut(s) 15, 222, 225, 1024, 2995, 3060, 3164
BspANI GGCC 4 cut(s) 149, 1585, 1760, 3090
BspCNI CTCAG 6 cut(s) 376, 557, 1033, 1143, 2843, 3539
BspFNI CGCG 2 cut(s) 17, 356
BspHI TCATGA 2 cut(s) 1468, 1897
BspLI GGNNCC 7 cut(s) 867, 1712, 1833, 2208, 3036, 3583, 3611
BspMAI CTGCAG 3 cut(s) 804, 1431, 1802
BspMI ACCTGC 3 cut(s) 115, 2095, 2231
BspT104I TTCGAA 2 cut(s) 100, 1136
BspT107I GGYRCC 1 cut(s) 3581
BspTNI GGTCTC 2 cut(s) 163, 3277
BsrDI GCAATG 1 cut(s) 3432
BsrFI RCCGGY 1 cut(s) 1537
BsrGI TGTACA 1 cut(s) 1000
BsrI ACTGG 3 cut(s) 869, 2967, 3009
BssAI RCCGGY 1 cut(s) 1537
BssECI CCNNGG 7 cut(s) 1755, 1916, 2196, 2937, 3030, 3085, 3091
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 1916, 3030, 3091
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 6 cut(s) 1756, 1873, 2197, 2538, 2671, 2938
Bst4CI ACNGT 8 cut(s) 59, 452, 1485, 1571, 2218, 2429, 2777, 3326
BstAPI GCANNNNNTGC 2 cut(s) 1948, 2530
BstAUI TGTACA 1 cut(s) 1000
BstBAI YACGTR 1 cut(s) 3306
BstBI TTCGAA 2 cut(s) 100, 1136
BstC8I GCNNGC 5 cut(s) 15, 482, 804, 1587, 1869
BstDSI CCRYGG 2 cut(s) 3085, 3091
BstFNI CGCG 2 cut(s) 17, 356
BstH2I RGCGCY 1 cut(s) 1886
BstHHI GCGC 3 cut(s) 19, 356, 1885
BstMAI GTCTC 5 cut(s) 160, 163, 1850, 2576, 3277
BstMWI GCNNNNNNNGC 7 cut(s) 10, 1098, 1708, 1829, 1948, 2530, 2789
BstNI CCWGG 6 cut(s) 1756, 1873, 2197, 2538, 2671, 2938
BstNSI RCATGY 4 cut(s) 1871, 2571, 2919, 3060
BstPAI GACNNNNGTC 1 cut(s) 2656
BstSCI CCNGG 8 cut(s) 1754, 1871, 2195, 2536, 2669, 2936, 3401, 3590
BstSFI CTRYAG 9 cut(s) 143, 612, 800, 1030, 1224, 1427, 1509, 1798, 3617
BstSLI GKGCMC 5 cut(s) 15, 513, 1674, 2794, 3241
BstUI CGCG 2 cut(s) 17, 356
BstV1I GCAGC 9 cut(s) 1397, 1487, 1689, 1935, 2028, 2042, 2343, 3261, 3266
BstV2I GAAGAC 1 cut(s) 3447
BstX2I RGATCY 4 cut(s) 865, 2502, 3034, 3609
BstXI CCANNNNNNTGG 1 cut(s) 2777
BstYI RGATCY 4 cut(s) 865, 2502, 3034, 3609
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 4 cut(s) 149, 1585, 1760, 3090
BtgI CCRYGG 2 cut(s) 3085, 3091
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 8 cut(s) 131, 696, 1193, 1365, 1599, 2782, 2960, 3331
BveI ACCTGC 3 cut(s) 115, 2095, 2231
Cac8I GCNNGC 5 cut(s) 15, 482, 804, 1587, 1869
CaiI CAGNNNCTG 1 cut(s) 1948
CciI TCATGA 2 cut(s) 1468, 1897
CfoI GCGC 3 cut(s) 19, 356, 1885
Cfr10I RCCGGY 1 cut(s) 1537
Cfr13I GGNCC 2 cut(s) 1758, 2992
CseI GACGC 1 cut(s) 2661
Csp6I GTAC 3 cut(s) 832, 1001, 3582
CspCI CAANNNNNGTGG 4 cut(s) 672, 707, 1750, 1785
CviQI GTAC 3 cut(s) 832, 1001, 3582
DriI GACNNNNNGTC 2 cut(s) 409, 727
EaeI YGGCCR 1 cut(s) 3088
Eam1105I GACNNNNNGTC 2 cut(s) 409, 727
Ecl136II GAGCTC 1 cut(s) 619
Eco130I CCWWGG 3 cut(s) 1916, 3030, 3091
Eco24I GRGCYC 2 cut(s) 621, 1713
Eco31I GGTCTC 2 cut(s) 163, 3277
Eco32I GATATC 1 cut(s) 2479
Eco47I GGWCC 1 cut(s) 2992
Eco47III AGCGCT 1 cut(s) 1884
Eco53kI GAGCTC 1 cut(s) 619
Eco57I CTGAAG 2 cut(s) 672, 2151
Eco88I CYCGRG 2 cut(s) 163, 2756
EcoICRI GAGCTC 1 cut(s) 619
EcoRI GAATTC 2 cut(s) 48, 628
EcoRII CCWGG 6 cut(s) 1754, 1871, 2195, 2536, 2669, 2936
EcoRV GATATC 1 cut(s) 2479
EcoT14I CCWWGG 3 cut(s) 1916, 3030, 3091
EcoT22I ATGCAT 3 cut(s) 1018, 1781, 3115
EcoT38I GRGCYC 2 cut(s) 621, 1713
ErhI CCWWGG 3 cut(s) 1916, 3030, 3091
Esp3I CGTCTC 1 cut(s) 1850
FalI AAGNNNNNCTT 4 cut(s) 2799, 2831, 3303, 3335
FaqI GGGAC 7 cut(s) 382, 857, 2438, 2900, 2954, 3077, 3514
FauI CCCGC 2 cut(s) 22, 3053
FblI GTMKAC 1 cut(s) 3221
FokI GGATG 9 cut(s) 44, 296, 1223, 2306, 2520, 2617, 2686, 3467, 3536
FriOI GRGCYC 2 cut(s) 621, 1713
FspBI CTAG 8 cut(s) 441, 807, 1715, 1850, 2060, 2421, 2549, 2564
GlaI GCGC 3 cut(s) 18, 355, 1884
GsuI CTGGAG 2 cut(s) 605, 3026
HaeII RGCGCY 1 cut(s) 1886
HaeIII GGCC 4 cut(s) 149, 1585, 1760, 3090
HapII CCGG 3 cut(s) 1538, 3402, 3591
HgaI GACGC 1 cut(s) 2661
HhaI GCGC 3 cut(s) 19, 356, 1885
Hin6I GCGC 3 cut(s) 17, 354, 1883
HinP1I GCGC 3 cut(s) 17, 354, 1883
HpaII CCGG 3 cut(s) 1538, 3402, 3591
HphI GGTGA 5 cut(s) 761, 1043, 3031, 3115, 3430
Hpy166II GTNNAC 4 cut(s) 511, 1145, 1672, 3222
Hpy8I GTNNAC 4 cut(s) 511, 1145, 1672, 3222
Hpy99I CGWCG 1 cut(s) 2265
HpyAV CCTTC 8 cut(s) 1187, 1952, 2383, 2407, 2624, 2839, 2887, 3352
HpyCH4III ACNGT 8 cut(s) 59, 452, 1485, 1571, 2218, 2429, 2777, 3326
HpyCH4IV ACGT 1 cut(s) 3305
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 1098, 1708, 1829, 1948, 2530, 2789
HpySE526I ACGT 1 cut(s) 3305
HspAI GCGC 3 cut(s) 17, 354, 1883
KpnI GGTACC 1 cut(s) 3585
LmnI GCTCC 9 cut(s) 624, 818, 1716, 1828, 1837, 2545, 2683, 2780, 2896
Lsp1109I GCAGC 9 cut(s) 1397, 1487, 1689, 1935, 2028, 2042, 2343, 3261, 3266
MaeI CTAG 8 cut(s) 441, 807, 1715, 1850, 2060, 2421, 2549, 2564
MaeII ACGT 1 cut(s) 3305
MfeI CAATTG 3 cut(s) 273, 2003, 3336
MflI RGATCY 4 cut(s) 865, 2502, 3034, 3609
MlsI TGGCCA 1 cut(s) 3090
MluNI TGGCCA 1 cut(s) 3090
MlyI GAGTC 9 cut(s) 45, 419, 639, 659, 737, 864, 2315, 2479, 2775
MmeI TCCRAC 4 cut(s) 426, 2023, 2271, 2743
Mox20I TGGCCA 1 cut(s) 3090
Mph1103I ATGCAT 3 cut(s) 1018, 1781, 3115
MroXI GAANNNNTTC 3 cut(s) 1893, 2191, 2559
MscI TGGCCA 1 cut(s) 3090
MseI TTAA 8 cut(s) 639, 947, 1518, 2232, 2355, 2615, 3099, 3396
MslI CAYNNNNRTG 3 cut(s) 2775, 2881, 3017
Msp20I TGGCCA 1 cut(s) 3090
MspA1I CMGCKG 2 cut(s) 44, 1024
MspI CCGG 3 cut(s) 1538, 3402, 3591
MspR9I CCNGG 8 cut(s) 1756, 1873, 2197, 2538, 2671, 2938, 3403, 3592
MunI CAATTG 3 cut(s) 273, 2003, 3336
Mva1269I GAATGC 2 cut(s) 1911, 2162
MvaI CCWGG 6 cut(s) 1756, 1873, 2197, 2538, 2671, 2938
MvnI CGCG 2 cut(s) 17, 356
MwoI GCNNNNNNNGC 7 cut(s) 10, 1098, 1708, 1829, 1948, 2530, 2789
NciI CCSGG 2 cut(s) 3403, 3592
NcoI CCATGG 1 cut(s) 3091
NlaIV GGNNCC 7 cut(s) 867, 1712, 1833, 2208, 3036, 3583, 3611
NmuCI GTSAC 6 cut(s) 37, 411, 2245, 3301, 3390, 3418
NsiI ATGCAT 3 cut(s) 1018, 1781, 3115
NspI RCATGY 4 cut(s) 1871, 2571, 2919, 3060
NspV TTCGAA 2 cut(s) 100, 1136
PaeI GCATGC 1 cut(s) 1871
PaeR7I CTCGAG 1 cut(s) 2756
PagI TCATGA 2 cut(s) 1468, 1897
PaqCI CACCTGC 1 cut(s) 2231
PctI GAATGC 2 cut(s) 1911, 2162
PdmI GAANNNNTTC 3 cut(s) 1893, 2191, 2559
PflMI CCANNNNNTGG 3 cut(s) 2026, 2290, 2445
PfoI TCCNGGA 1 cut(s) 2536
PleI GAGTC 9 cut(s) 44, 418, 639, 659, 736, 863, 2315, 2478, 2774
PpsI GAGTC 9 cut(s) 44, 418, 639, 659, 736, 863, 2315, 2478, 2774
Ppu21I YACGTR 1 cut(s) 3306
PshAI GACNNNNGTC 1 cut(s) 2656
PsiI TTATAA 2 cut(s) 336, 2951
Psp124BI GAGCTC 1 cut(s) 621
Psp6I CCWGG 6 cut(s) 1754, 1871, 2195, 2536, 2669, 2936
PspGI CCWGG 6 cut(s) 1754, 1871, 2195, 2536, 2669, 2936
PspN4I GGNNCC 7 cut(s) 867, 1712, 1833, 2208, 3036, 3583, 3611
PspPI GGNCC 2 cut(s) 1758, 2992
PsrI GAACNNNNNNTAC 2 cut(s) 1101, 1133
PstI CTGCAG 3 cut(s) 804, 1431, 1802
PstNI CAGNNNCTG 1 cut(s) 1948
PsuI RGATCY 4 cut(s) 865, 2502, 3034, 3609
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 3 cut(s) 833, 1002, 3583
RsaNI GTAC 3 cut(s) 832, 1001, 3582
RseI CAYNNNNRTG 3 cut(s) 2775, 2881, 3017
SacI GAGCTC 1 cut(s) 621
SaqAI TTAA 8 cut(s) 639, 947, 1518, 2232, 2355, 2615, 3099, 3396
Sau96I GGNCC 2 cut(s) 1758, 2992
SchI GAGTC 9 cut(s) 45, 419, 639, 659, 737, 864, 2315, 2479, 2775
ScrFI CCNGG 8 cut(s) 1756, 1873, 2197, 2538, 2671, 2938, 3403, 3592
SfcI CTRYAG 9 cut(s) 143, 612, 800, 1030, 1224, 1427, 1509, 1798, 3617
Sfr274I CTCGAG 1 cut(s) 2756
SfuI TTCGAA 2 cut(s) 100, 1136
SinI GGWCC 1 cut(s) 2992
SlaI CTCGAG 1 cut(s) 2756
SmiMI CAYNNNNRTG 3 cut(s) 2775, 2881, 3017
SmlI CTYRAG 3 cut(s) 525, 2114, 2756
SmoI CTYRAG 3 cut(s) 525, 2114, 2756
SphI GCATGC 1 cut(s) 1871
SsiI CCGC 7 cut(s) 15, 222, 225, 1024, 2995, 3060, 3164
SspI AATATT 1 cut(s) 463
SspMI CTAG 8 cut(s) 441, 807, 1715, 1850, 2060, 2421, 2549, 2564
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 8 cut(s) 1754, 1871, 2195, 2536, 2669, 2936, 3401, 3590
StyI CCWWGG 3 cut(s) 1916, 3030, 3091
TaaI ACNGT 8 cut(s) 59, 452, 1485, 1571, 2218, 2429, 2777, 3326
TaiI ACGT 1 cut(s) 3308
TaqI TCGA 7 cut(s) 24, 100, 914, 1136, 1404, 2260, 2757
TatI WGTACW 1 cut(s) 1000
TauI GCSGC 1 cut(s) 225
Tru1I TTAA 8 cut(s) 639, 947, 1518, 2232, 2355, 2615, 3099, 3396
Tru9I TTAA 8 cut(s) 639, 947, 1518, 2232, 2355, 2615, 3099, 3396
TscAI CASTG 8 cut(s) 138, 696, 1193, 1365, 1599, 2782, 2967, 3331
TseFI GTSAC 6 cut(s) 37, 411, 2245, 3301, 3390, 3418
TseI GCWGC 9 cut(s) 1385, 1475, 1702, 1948, 2030, 2041, 2331, 3254, 3274
Tsp45I GTSAC 6 cut(s) 37, 411, 2245, 3301, 3390, 3418
TspGWI ACGGA 4 cut(s) 2278, 2333, 3295, 3452
TspRI CASTG 8 cut(s) 138, 696, 1193, 1365, 1599, 2782, 2967, 3331
Van91I CCANNNNNTGG 3 cut(s) 2026, 2290, 2445
VneI GTGCAC 2 cut(s) 509, 1670
VpaK11BI GGWCC 1 cut(s) 2992
XceI RCATGY 4 cut(s) 1871, 2571, 2919, 3060
XhoI CTCGAG 1 cut(s) 2756
XmiI GTMKAC 1 cut(s) 3221
XmnI GAANNNNTTC 3 cut(s) 1893, 2191, 2559
XspI CTAG 8 cut(s) 441, 807, 1715, 1850, 2060, 2421, 2549, 2564
Zsp2I ATGCAT 3 cut(s) 1018, 1781, 3115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.