RchiOBHm_Chr4g0422831

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010154
Physical Location & Seq
Forward (+)
48406373 .. 48413845
7473 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39227

Sequence Viewer

Length: 3987 bp
ATGAAAATGAACTTGCTTAGTGAATCGGTGGAAGCTGTAGCTAGCTGTTCTACTACTGCTTTGGTTTCGTCTCATAATCAAGAGAGATTAGACCTCAAGTCCATTCGGGCGGTTGTCATTTCAAGCAATCAATGCATTCTCAAACTCATAGCAAATGACAAAACTAGAAACTCTATAAGGGTGAGATGTATCTCCAAGCTTCAAAATCAGAAACAGGATTTCTTCGAATTCTCTGACCAGTCTGTCATATCAAATCTTTACTGGGGTATTGACAGCATAGAAGCTGCAATTACCGCCGAATGGCCAGAAGAGAAAGCCGCTCAGCTGAGGAAGGCAGAGCAGATGCTACAAGTGCCCGCGCTACTCGATGAGGATGGAGTCACAGCTGGAATTCCGAACAGTTATTTGGTGTGTTGCTCATACTTTTACCTCTCTGTTGTTCGAAAACTCCAAGAGGATGAGTGGCAGGTTGCACTGCATTTCCTACAGGCCGTGCTGGTCTCCCCGAGACTTGTTCAGACAGAATTTGCGCATGACCTTTATGAAAGTCTTTTCCCGACTTGCGGCGGTTCTGAAAGACAAGAAATAAGAGCGAGTAAGAGCTTGGAATCAATTGACAAGGATGAAGCAATAAGGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGCGCGACTTCTCAGAGTCAAGGTGGAGGATACAGAGGCATTTTGTCCCCTGACAAAGAGTCACTATATTCAATACATGGGAAATCCACTAGGTCGGACTGTTCAAATAATATTGAGCATGACCACAGCTTGCACACTCAATGGAATTATGAAAAAGTGCACCCTTTTGATCCTCAAGAAGATATTGGCATCGAAGATGAACTGAAACAATCCATCCACATCTCAGAATTTGAAGAATATGGGAAATCCAAAAATGATTTGAATCAAGCAACAGAGTTTCATGTTAAAACTAGAGAGCTCCAGAGGAATTCAAATATTAAAAGACTCCAAGATTTGCTGAATGACTCTCAATCAGACACACCAACTTCAGTGGATTCATGCTCTGATTATTCAGATTATGACATTGAGTCAGAGGTCATTGATGGTGGTGAGTGCTCAACCAGAGATGCAACAATAGGTGCAGATTTTTCCAAGAAACTGCAGGCTACTAGCTCCACATCAGACCCAAAGTACGAAGCATTATCTTTTTCACAAGTCTGTCAGGATCCAGTCCCTAACGAAGTCAGTCAAGTAAATAACTCTATGATTCTTTCGAGCAGATTCACAAATTCCATCAATGGTTTATTAACTATTTCAGAGCATAGAGATAAAAGATCAAAGACCCATCAGAATTTATATGTACAAAAGGAGTATGCATCTCAGCGGAACTATAGGATCAACCAAAGGGATCACCAAAGAAGTGTTGCAAGGAAAAAACATAGTTCTCATAGCCAGAAAAGCTCTATTGAACTTCGCTTACATTCCACAAAAGATTCGAAAAGTGAACTACTGAGTATTACTGAAAAAGCAATTTCAAAACTATTTCAGTGGGAAGGATTGGGAAAATGGGATGAAGATTATGCAGTAGAAGTTACAACAATATATCAAATACTATGCAACAAAAAAGGAGAGAAATGTGCTGTCTTGAAAGACATGATATTGGATCAACTGCTCATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTGATAAGGGTATCAGTGTCTATACTAACTACTATTGTTGCAGCTAATAAGTCAGTTATCGAGGACATAAAGAAGAAAGGTCTGCAGTTATCTGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCATCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTGTTGGGGGTCGTGTGCTCTCCAAACAGTTATAAGGGTAGGCCAGCATCAGTGCCAACACCTCTGACAGCATCACTAATGATTATTGGAGTTTTAGTATCTTCATTTGACCATGCCACAAATAATGTGCACTTGGCTGAAATCAGTTATCCCAATGTGCTGCATGGGCTCCTAGATGTTGCAAGAGATAGTAACATTGAAGAATTGATCTCCTGGGCCACTATTCTTGTCAAATGCATACAATACGATGGAAACTGCAGAAGATATATATCAAGATTAGCTCCAATGGCTCCATTTTCCCGTCTCCTAGAATGTAAGATGAAGCATGCCAGGTGCATAGCGCTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCGGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACGAAGATTATGAACTCACTCATGCTTTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCATTTAGATACACTAGATAACTCCTCCTGTAAGAGTGCATTTACAGAAGAAGCTATGCAGGTTCTCCTCAAGTTGGTCGCATCTGAAGAAGGTTCCACTACACAGCACTTATCTGCATTCATCTTATCAAATCTCGGTGGAACATATTCATGGGCAGGGGAACCATATACAGTAGCATGGTTGGTTAAAAAGGCAGGTGTGACTTCCTCGTATCGACGGAATATGATAAAAACTATCCATTGGTTGGATGATTGCTTAGAGGATTCTGGCACGGACTCATGGTGCAGCAAAATTTCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTGATTCTTTAGTGAAGGGTCTAAAGAGCACAAGAAGGAAGGTTTCTAGGGACTGTCTAATAGCCATAGCGTGGCTTGGGTTTGAAATTGCAAAGAGTCCAGATATCATCAGAAATTCTGCCTGTGAGATCTTACTTAGTGGAGTTGAGCAATTTTTGCATCCTGGATTGGAGCTAGAAGAACGAGTTCTAGCATGTCTCTGCATATATAACTACGCTTCTGGTAAAGGGATGAAAAAGTTAATTCATTTCTCGGAAGGAGTGAGAGAATCACTAAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGATCACGAATTTCTTGTGTTCATACCCAAATTCTCGAGGTTGGAGTCAATTACAGTGGAGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGCTACTCAGATGGTTCTGTGAAGGTCTGGAACATAAAAGGTCAGTCAGCCACACTCGTATGGGACATGAAGGAGCACAAAAAGGCAGTTACATGCTTTTCACTACTTGAATCCAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTATAAGGGTCTGGCAAGTGGTCCGCAAAAAACTGGAGTGCATTGAGGTGATAGAAACCAAGGAACCAATTCGGCATCTGAATACATGCGGGGACATGATATTTGCAATTACCCGTGGCCATGGAATTAAGGTGATTGATGCATCAAGAAAGGTCAAAGATAATTGCACGAATAAAAAGGTAAAGTGTATGGCGGTGGTTCAGGGGAAGATTTATGCAGGTTGCAAGGATTCAAGCATACAGGAGTTGTCTACAACAAGCAATCGGGCACAAGAGATCAAAGCAGCATCAAAATTTTGGAGGCTGCAACGGAGACCCATAAATGCAGTTGTCACATACAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGCAAGGTTCAAACTTGAAGGAGTGGAAAAGACACGGCAGACCCCAAATGTCACTTAAAACCGGGAAAAGAGAAAAGGTGACGGCAATGGGAATAACGGAAGACTTCATCTACCTCAACTGCAACTCATCAACAAACAGCATCCAGATATGGCTAAGAGGGACACAGCAGAAAGTGGGCAGAATCTCAGCAGGAAGCAGAATTACGAGCATCCTCACAGCCAACGACATTATCTTGTGTGGTACCGAAACCGGGCTAATCAAGGGATGGATCCCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1328

Amino Acids

149.21

Weight (kDa)

8.38

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 35 - 186 2.1e-28 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 505 - 666 5.4e-48 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 670 - 1022 5.2e-56 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_TEP1_2nd PF25047 1052 - 1119 6.8e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 1061 - 1137 5e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1063 - 1196 2.3e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1064 - 1136 1.9e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1065 - 1138 5.7e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 675, 1944, 3317
AarI CACCTGC 1 cut(s) 2600
AasI GACNNNNNNGTC 1 cut(s) 242
Acc16I TGCGCA 1 cut(s) 531
Acc36I ACCTGC 4 cut(s) 457, 2464, 2600, 3545
Acc65I GGTACC 1 cut(s) 3947
AccB1I GGYRCC 1 cut(s) 3947
AccB7I CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 3587
AccII CGCG 2 cut(s) 359, 695
AclWI GGATC 9 cut(s) 854, 1229, 1242, 1412, 1425, 1680, 3331, 3970, 3983
AcoI YGGCCR 2 cut(s) 302, 3454
AcuI CTGAAG 2 cut(s) 1041, 2520
AfaI GTAC 3 cut(s) 1202, 1371, 3949
AfeI AGCGCT 1 cut(s) 2253
AfiI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3957
AhdI GACNNNNNGTC 3 cut(s) 97, 748, 1096
AjnI CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3302
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
Alw21I GWGCWC 8 cut(s) 852, 990, 1127, 1822, 1931, 2043, 2774, 3267
Alw26I GTCTC 6 cut(s) 75, 502, 505, 2219, 2945, 3643
Alw44I GTGCAC 2 cut(s) 848, 2039
AlwI GGATC 9 cut(s) 854, 1229, 1242, 1412, 1425, 1680, 3331, 3970, 3983
AlwNI CAGNNNCTG 1 cut(s) 2317
Ama87I CYCGRG 2 cut(s) 505, 3122
Aor51HI AGCGCT 1 cut(s) 2253
AoxI GGCC 5 cut(s) 302, 489, 1952, 2127, 3454
ApaLI GTGCAC 2 cut(s) 848, 2039
ArsI GACNNNNNNTTYG 2 cut(s) 3422, 3454
Asp700I GAANNNNTTC 4 cut(s) 2262, 2560, 2928, 3405
Asp718I GGTACC 1 cut(s) 3947
AspLEI GCGC 4 cut(s) 361, 532, 695, 2254
AspS9I GGNCC 2 cut(s) 2127, 3358
AsuC2I CCSGG 2 cut(s) 3769, 3958
AsuHPI GGTGA 6 cut(s) 193, 1130, 1412, 3397, 3481, 3796
AsuII TTCGAA 3 cut(s) 225, 442, 1505
AsuNHI GCTAGC 1 cut(s) 41
AvaI CYCGRG 2 cut(s) 505, 3122
AvaII GGWCC 1 cut(s) 3358
BaeGI GKGCMC 5 cut(s) 357, 852, 2043, 3160, 3607
BalI TGGCCA 2 cut(s) 304, 3456
BamHI GGATCC 2 cut(s) 1234, 3975
BanI GGYRCC 1 cut(s) 3947
BanII GRGCYC 2 cut(s) 990, 2082
BarI GAAGNNNNNNTAC 4 cut(s) 1681, 1713, 3892, 3924
BbsI GAAGAC 1 cut(s) 3813
Bbv12I GWGCWC 8 cut(s) 852, 990, 1127, 1822, 1931, 2043, 2774, 3267
BbvCI CCTCAGC 1 cut(s) 326
BceAI ACGGC 3 cut(s) 476, 3757, 3804
BciT130I CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3304
BciVI GTATCC 1 cut(s) 713
BcnI CCSGG 2 cut(s) 3769, 3958
BcoDI GTCTC 6 cut(s) 75, 502, 505, 2219, 2945, 3643
BfmI CTRYAG 8 cut(s) 36, 485, 1169, 1399, 1796, 1878, 2167, 3983
BfoI RGCGCY 1 cut(s) 2255
BfuAI ACCTGC 4 cut(s) 457, 2464, 2600, 3545
BfuI GTATCC 1 cut(s) 713
BglII AGATCT 1 cut(s) 2871
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 7 cut(s) 2125, 2242, 2907, 3040, 3304, 3769, 3958
Bme18I GGWCC 1 cut(s) 3358
BmeRI GACNNNNNGTC 3 cut(s) 97, 748, 1096
BmeT110I CYCGRG 2 cut(s) 505, 3122
BmgT120I GGNCC 2 cut(s) 2127, 3358
BmiI GGNNCC 8 cut(s) 1236, 2081, 2202, 2509, 2577, 3402, 3949, 3977
BmrFI CCNGG 7 cut(s) 2125, 2242, 2907, 3040, 3304, 3769, 3958
BmrI ACTGGG 1 cut(s) 271
BmtI GCTAGC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 271
BoxI GACNNNNGTC 1 cut(s) 3025
BpiI GAAGAC 1 cut(s) 3813
BpmI CTGGAG 2 cut(s) 974, 3392
Bpu10I CCTNAGC 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 3 cut(s) 225, 442, 1505
BpuEI CTTGAG 3 cut(s) 80, 849, 2468
BpuMI CCSGG 2 cut(s) 3769, 3958
BsaBI GATNNNNATC 2 cut(s) 951, 2179
BsaI GGTCTC 2 cut(s) 505, 3643
BsaJI CCNNGG 6 cut(s) 2124, 2285, 3303, 3396, 3451, 3457
BsaXI ACNNNNNCTCC 2 cut(s) 1992, 2022
Bsc4I CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3957
Bse118I RCCGGY 1 cut(s) 1906
Bse1I ACTGG 5 cut(s) 238, 266, 1238, 3333, 3375
Bse3DI GCAATG 1 cut(s) 3798
Bse8I GATNNNNATC 2 cut(s) 951, 2179
BseBI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3304
BseDI CCNNGG 6 cut(s) 2124, 2285, 3303, 3396, 3451, 3457
BseJI GATNNNNATC 2 cut(s) 951, 2179
BseLI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3957
BseMI GCAATG 1 cut(s) 3798
BseMII CTCAG 8 cut(s) 317, 335, 716, 927, 1403, 1511, 3210, 3906
BseNI ACTGG 5 cut(s) 238, 266, 1238, 3333, 3375
BseRI GAGGAG 4 cut(s) 1721, 2428, 2471, 3065
BseSI GKGCMC 5 cut(s) 357, 852, 2043, 3160, 3607
BsgI GTGCAG 2 cut(s) 1170, 2719
Bsh1236I CGCG 2 cut(s) 359, 695
BshFI GGCC 5 cut(s) 304, 491, 1954, 2129, 3456
BshNI GGYRCC 1 cut(s) 3947
BsiHKAI GWGCWC 8 cut(s) 852, 990, 1127, 1822, 1931, 2043, 2774, 3267
BsiHKCI CYCGRG 2 cut(s) 505, 3122
BsiSI CCGG 3 cut(s) 1907, 3768, 3957
BslFI GGGAC 7 cut(s) 721, 1226, 2807, 3266, 3320, 3443, 3880
BslI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3957
BsmAI GTCTC 6 cut(s) 75, 502, 505, 2219, 2945, 3643
BsmBI CGTCTC 2 cut(s) 75, 2219
BsmFI GGGAC 7 cut(s) 721, 1226, 2807, 3266, 3320, 3443, 3880
BsmI GAATGC 3 cut(s) 135, 2280, 2531
BsnI GGCC 5 cut(s) 304, 491, 1954, 2129, 3456
Bso31I GGTCTC 2 cut(s) 505, 3643
BsoBI CYCGRG 2 cut(s) 505, 3122
Bsp119I TTCGAA 3 cut(s) 225, 442, 1505
Bsp1407I TGTACA 1 cut(s) 1369
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 3457
BspANI GGCC 5 cut(s) 304, 491, 1954, 2129, 3456
BspCNI CTCAG 8 cut(s) 318, 334, 715, 926, 1402, 1512, 3209, 3905
BspFNI CGCG 2 cut(s) 359, 695
BspHI TCATGA 2 cut(s) 1837, 2266
BspLI GGNNCC 8 cut(s) 1236, 2081, 2202, 2509, 2577, 3402, 3949, 3977
BspMAI CTGCAG 3 cut(s) 1173, 1800, 2171
BspMI ACCTGC 4 cut(s) 457, 2464, 2600, 3545
BspOI GCTAGC 1 cut(s) 45
BspPI GGATC 9 cut(s) 854, 1229, 1242, 1412, 1425, 1680, 3331, 3970, 3983
BspT104I TTCGAA 3 cut(s) 225, 442, 1505
BspT107I GGYRCC 1 cut(s) 3947
BspTNI GGTCTC 2 cut(s) 505, 3643
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 3798
BsrFI RCCGGY 1 cut(s) 1906
BsrGI TGTACA 1 cut(s) 1369
BsrI ACTGG 5 cut(s) 238, 266, 1238, 3333, 3375
BssAI RCCGGY 1 cut(s) 1906
BssECI CCNNGG 6 cut(s) 2124, 2285, 3303, 3396, 3451, 3457
BssT1I CCWWGG 3 cut(s) 2285, 3396, 3457
Bst2UI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3304
Bst4CI ACNGT 8 cut(s) 401, 791, 1854, 1940, 2587, 2798, 3143, 3692
Bst6I CTCTTC 1 cut(s) 303
BstAPI GCANNNNNTGC 3 cut(s) 132, 2317, 2899
BstAUI TGTACA 1 cut(s) 1369
BstBI TTCGAA 3 cut(s) 225, 442, 1505
BstC8I GCNNGC 6 cut(s) 43, 357, 821, 1173, 1956, 2238
BstDSI CCRYGG 2 cut(s) 3451, 3457
BstFNI CGCG 2 cut(s) 359, 695
BstH2I RGCGCY 1 cut(s) 2255
BstHHI GCGC 4 cut(s) 361, 532, 695, 2254
BstMAI GTCTC 6 cut(s) 75, 502, 505, 2219, 2945, 3643
BstMWI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3155
BstNI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3304
BstNSI RCATGY 4 cut(s) 2240, 2940, 3285, 3426
BstPAI GACNNNNGTC 1 cut(s) 3025
BstSCI CCNGG 7 cut(s) 2123, 2240, 2905, 3038, 3302, 3767, 3956
BstSFI CTRYAG 8 cut(s) 36, 485, 1169, 1399, 1796, 1878, 2167, 3983
BstSLI GKGCMC 5 cut(s) 357, 852, 2043, 3160, 3607
BstUI CGCG 2 cut(s) 359, 695
BstV2I GAAGAC 1 cut(s) 3813
BstX2I RGATCY 3 cut(s) 1234, 2871, 3975
BstYI RGATCY 3 cut(s) 1234, 2871, 3975
BsuI GTATCC 1 cut(s) 713
BsuRI GGCC 5 cut(s) 304, 491, 1954, 2129, 3456
BtgI CCRYGG 2 cut(s) 3451, 3457
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 8 cut(s) 473, 1065, 1562, 1734, 1968, 3148, 3326, 3697
BveI ACCTGC 4 cut(s) 457, 2464, 2600, 3545
Cac8I GCNNGC 6 cut(s) 43, 357, 821, 1173, 1956, 2238
CaiI CAGNNNCTG 1 cut(s) 2317
CciI TCATGA 2 cut(s) 1837, 2266
CfoI GCGC 4 cut(s) 361, 532, 695, 2254
Cfr10I RCCGGY 1 cut(s) 1906
Cfr13I GGNCC 2 cut(s) 2127, 3358
CseI GACGC 1 cut(s) 3030
Csp6I GTAC 3 cut(s) 1201, 1370, 3948
CspCI CAANNNNNGTGG 6 cut(s) 1041, 1076, 2119, 2154, 3124, 3159
CviQI GTAC 3 cut(s) 1201, 1370, 3948
DrdI GACNNNNNNGTC 1 cut(s) 242
DriI GACNNNNNGTC 3 cut(s) 97, 748, 1096
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 302, 3454
Eam1104I CTCTTC 1 cut(s) 303
Eam1105I GACNNNNNGTC 3 cut(s) 97, 748, 1096
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 3 cut(s) 2285, 3396, 3457
Eco24I GRGCYC 2 cut(s) 990, 2082
Eco31I GGTCTC 2 cut(s) 505, 3643
Eco32I GATATC 1 cut(s) 2848
Eco47I GGWCC 1 cut(s) 3358
Eco47III AGCGCT 1 cut(s) 2253
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 2 cut(s) 1041, 2520
Eco88I CYCGRG 2 cut(s) 505, 3122
EcoICRI GAGCTC 1 cut(s) 988
EcoRI GAATTC 3 cut(s) 227, 390, 997
EcoRII CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3302
EcoRV GATATC 1 cut(s) 2848
EcoT14I CCWWGG 3 cut(s) 2285, 3396, 3457
EcoT22I ATGCAT 4 cut(s) 137, 1387, 2150, 3481
EcoT38I GRGCYC 2 cut(s) 990, 2082
ErhI CCWWGG 3 cut(s) 2285, 3396, 3457
Esp3I CGTCTC 2 cut(s) 75, 2219
FalI AAGNNNNNCTT 4 cut(s) 3165, 3197, 3669, 3701
FaqI GGGAC 7 cut(s) 721, 1226, 2807, 3266, 3320, 3443, 3880
FauI CCCGC 2 cut(s) 364, 3419
FblI GTMKAC 1 cut(s) 3587
FriOI GRGCYC 2 cut(s) 990, 2082
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 4 cut(s) 360, 531, 694, 2253
GsuI CTGGAG 2 cut(s) 974, 3392
HaeII RGCGCY 1 cut(s) 2255
HaeIII GGCC 5 cut(s) 304, 491, 1954, 2129, 3456
HapII CCGG 3 cut(s) 1907, 3768, 3957
HgaI GACGC 1 cut(s) 3030
HhaI GCGC 4 cut(s) 361, 532, 695, 2254
Hin6I GCGC 4 cut(s) 359, 530, 693, 2252
HinP1I GCGC 4 cut(s) 359, 530, 693, 2252
HindIII AAGCTT 1 cut(s) 197
HpaII CCGG 3 cut(s) 1907, 3768, 3957
HphI GGTGA 6 cut(s) 193, 1130, 1412, 3397, 3481, 3796
Hpy166II GTNNAC 4 cut(s) 850, 1514, 2041, 3588
Hpy8I GTNNAC 4 cut(s) 850, 1514, 2041, 3588
Hpy99I CGWCG 1 cut(s) 2634
HpyCH4III ACNGT 8 cut(s) 401, 791, 1854, 1940, 2587, 2798, 3143, 3692
HpyF10VI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3155
HspAI GCGC 4 cut(s) 359, 530, 693, 2252
KpnI GGTACC 1 cut(s) 3951
LmnI GCTCC 9 cut(s) 993, 1187, 2085, 2197, 2206, 2914, 3052, 3146, 3262
MbiI CCGCTC 1 cut(s) 320
MfeI CAATTG 3 cut(s) 612, 2372, 3702
MflI RGATCY 3 cut(s) 1234, 2871, 3975
MlsI TGGCCA 2 cut(s) 304, 3456
MluNI TGGCCA 2 cut(s) 304, 3456
MlyI GAGTC 9 cut(s) 387, 715, 758, 1008, 1028, 1106, 2684, 2848, 3141
MmeI TCCRAC 4 cut(s) 765, 2392, 2640, 3109
Mox20I TGGCCA 2 cut(s) 304, 3456
Mph1103I ATGCAT 4 cut(s) 137, 1387, 2150, 3481
MroXI GAANNNNTTC 4 cut(s) 2262, 2560, 2928, 3405
MscI TGGCCA 2 cut(s) 304, 3456
MseI TTAA 9 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 2984, 3465, 3762
MslI CAYNNNNRTG 3 cut(s) 2563, 3247, 3383
Msp20I TGGCCA 2 cut(s) 304, 3456
MspA1I CMGCKG 3 cut(s) 325, 386, 1393
MspI CCGG 3 cut(s) 1907, 3768, 3957
MspR9I CCNGG 7 cut(s) 2125, 2242, 2907, 3040, 3304, 3769, 3958
MunI CAATTG 3 cut(s) 612, 2372, 3702
Mva1269I GAATGC 3 cut(s) 135, 2280, 2531
MvaI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3304
MvnI CGCG 2 cut(s) 359, 695
MwoI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3155
NciI CCSGG 2 cut(s) 3769, 3958
NcoI CCATGG 1 cut(s) 3457
NheI GCTAGC 1 cut(s) 41
NlaIV GGNNCC 8 cut(s) 1236, 2081, 2202, 2509, 2577, 3402, 3949, 3977
NmuCI GTSAC 6 cut(s) 379, 750, 2614, 3667, 3756, 3784
NsbI TGCGCA 1 cut(s) 531
NsiI ATGCAT 4 cut(s) 137, 1387, 2150, 3481
NspI RCATGY 4 cut(s) 2240, 2940, 3285, 3426
NspV TTCGAA 3 cut(s) 225, 442, 1505
PaeI GCATGC 1 cut(s) 2240
PaeR7I CTCGAG 1 cut(s) 3122
PagI TCATGA 2 cut(s) 1837, 2266
PaqCI CACCTGC 1 cut(s) 2600
PctI GAATGC 3 cut(s) 135, 2280, 2531
PdmI GAANNNNTTC 4 cut(s) 2262, 2560, 2928, 3405
PflMI CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
PfoI TCCNGGA 1 cut(s) 2905
PleI GAGTC 9 cut(s) 386, 714, 757, 1008, 1028, 1105, 2684, 2847, 3140
PpsI GAGTC 9 cut(s) 386, 714, 757, 1008, 1028, 1105, 2684, 2847, 3140
PshAI GACNNNNGTC 1 cut(s) 3025
PsiI TTATAA 3 cut(s) 675, 1944, 3317
Psp124BI GAGCTC 1 cut(s) 990
Psp6I CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3302
PspGI CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3302
PspN4I GGNNCC 8 cut(s) 1236, 2081, 2202, 2509, 2577, 3402, 3949, 3977
PspPI GGNCC 2 cut(s) 2127, 3358
PsrI GAACNNNNNNTAC 2 cut(s) 1470, 1502
PstI CTGCAG 3 cut(s) 1173, 1800, 2171
PstNI CAGNNNCTG 1 cut(s) 2317
PsuI RGATCY 3 cut(s) 1234, 2871, 3975
PvuII CAGCTG 2 cut(s) 325, 386
RsaI GTAC 3 cut(s) 1202, 1371, 3949
RsaNI GTAC 3 cut(s) 1201, 1370, 3948
RseI CAYNNNNRTG 3 cut(s) 2563, 3247, 3383
SacI GAGCTC 1 cut(s) 990
SaqAI TTAA 9 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 2984, 3465, 3762
Sau96I GGNCC 2 cut(s) 2127, 3358
SchI GAGTC 9 cut(s) 387, 715, 758, 1008, 1028, 1106, 2684, 2848, 3141
ScrFI CCNGG 7 cut(s) 2125, 2242, 2907, 3040, 3304, 3769, 3958
SfcI CTRYAG 8 cut(s) 36, 485, 1169, 1399, 1796, 1878, 2167, 3983
Sfr274I CTCGAG 1 cut(s) 3122
SfuI TTCGAA 3 cut(s) 225, 442, 1505
SinI GGWCC 1 cut(s) 3358
SlaI CTCGAG 1 cut(s) 3122
SmiMI CAYNNNNRTG 3 cut(s) 2563, 3247, 3383
SmlI CTYRAG 4 cut(s) 95, 864, 2483, 3122
SmoI CTYRAG 4 cut(s) 95, 864, 2483, 3122
SphI GCATGC 1 cut(s) 2240
SspI AATATT 2 cut(s) 802, 1006
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 7 cut(s) 2123, 2240, 2905, 3038, 3302, 3767, 3956
StyI CCWWGG 3 cut(s) 2285, 3396, 3457
TaaI ACNGT 8 cut(s) 401, 791, 1854, 1940, 2587, 2798, 3143, 3692
TaqI TCGA 9 cut(s) 225, 366, 442, 882, 1283, 1505, 1773, 2629, 3123
TatI WGTACW 1 cut(s) 1369
TauI GCSGC 2 cut(s) 320, 567
Tru1I TTAA 9 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 2984, 3465, 3762
Tru9I TTAA 9 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 2984, 3465, 3762
TscAI CASTG 8 cut(s) 480, 1065, 1562, 1734, 1968, 3148, 3333, 3697
TseFI GTSAC 6 cut(s) 379, 750, 2614, 3667, 3756, 3784
Tsp45I GTSAC 6 cut(s) 379, 750, 2614, 3667, 3756, 3784
TspGWI ACGGA 4 cut(s) 2647, 2702, 3661, 3818
TspRI CASTG 8 cut(s) 480, 1065, 1562, 1734, 1968, 3148, 3333, 3697
Van91I CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
VneI GTGCAC 2 cut(s) 848, 2039
VpaK11BI GGWCC 1 cut(s) 3358
XceI RCATGY 4 cut(s) 2240, 2940, 3285, 3426
XhoI CTCGAG 1 cut(s) 3122
XmiI GTMKAC 1 cut(s) 3587
XmnI GAANNNNTTC 4 cut(s) 2262, 2560, 2928, 3405
Zsp2I ATGCAT 4 cut(s) 137, 1387, 2150, 3481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.