FvH4_4g18342

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010154
Physical Location & Seq
Forward (+)
22273111 .. 22280159
7049 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18342.t1

Sequence Viewer

Length: 3984 bp
ATGAAAATGAACTTGGTTGGTGACTCGATCGAAGCTGTAGCTAGCTGCTCTCGTACAGCTTTGGTTTCGTCTCATAATCAAGAGAGACTAGACCTCAAGTCCATTCGGGCAGTTGTCATATCAGTCAATCAATGCATTCTCAAACTCATAGCAAATGCCAAAACTAGAAACTCTATAAGGGTGAGATGTACCTCCAAGCTTCAAAATCAGAAACAGGATTTCTTTGAATTCTCTGACCAGTCTGTGATATCAAATCTTTATTGGGGAATTGACAGCATTGAGGCGGCAATTATCACTGAATGGCCAGAACAGAAGGCTGCTCAGCTGAGGAAGGCAGAGCAGATGCTACAGGTGCCGGCACTACTCGAGGAGGATGGAGTCACAGCTGGAATTTCAAACAGTTACTTGGTGTGCTGCTCGTATTTTTACCTCTCAGTGGTTCGAAAACTCCAAGAGGATGAGTGGCAGGTTGCACTGCATTTTCTACAGGCCGTGCTGGTCTCACCGAGACTTGTTCAGACGGAATTTGCACATGATCTTTATGAAAGTGTTTTCCCAACTTGCGCTGGTCCTGAAAGACAGGAAATAAGAGAGAGTAAGAGCTTGGAATCAATTGACAAGGATGAAGCAACAATGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGGGAGACTCCTCAGGGTCAAGGTGGAGGATACAGAGACATTTTGTCACCTGACAAAGAATCAATATATTCTCTGCACGGGAGATCCAATAGGTTGGACTATTCAAATAAAACTGGGCATGAGTACAACTTGCATACTCAATGGAATTATGGAAAGGTGCACCCTTTAGACCCTCATGAAGATAGTATCATAGAAGATGGACTAAAAACTTCCATACACATCTCAGAATTTGAAGAATATGGGAAACTCACAAATGATTTGAATCCAGCAACAGAGCTTCATGTTAAAACTAGAGAGGTCCAAAGGAATTTAAGTATTAAAAGACTCCAAGATGTGCTGGATGACTCTCAATCAGACTCACCAACTTCAGTGGATTCATGCTCTGATTATTCAGCTCATGACATTGAGTCAGAGGTCATTGATGGTGGTGAGTGCTTAAGCAGAACAGCAAGCATAGGTGCAGATTTTCCCAAGAAACTGCAGGCTCCTAGCTCCACATCAGACCCAGAGTGCGAAGCACAATCTTTTTCACGAGTCTGTCAGGATCCTATCCCTAACGAAGTCATTCAAGTAAATAACTCTATGATTCTTTCAAGGAGATTTACGAATTCCATCAATGGTTTATTAAGTATTTCAGAGCATAGAGATAAAAGATCAAAGCAGAATTCATATGTACAAAAGGAGTGTGCATCTCAGCAGAACTATAGGATCAACCAAAGGGATCACCAAAGAAGTATTGCAAGGAAAAAACATAGTTCTCGTAGCCAGCAAAGCTCTATTGAACTTCGCCTACATTCCACAAAAGACTCAAAAAGTGAACTACTGAGTATTACCGAAAAAGCAATTTCAAAACTATTTCACTGGGAAGGATTGGGGAAATGGGATGAAGACTATGCTGTTGAAGTTACAACCATCTATCAAATATTATGCAACAAAAAAGGAGAAAAATGTGCCGTCTTGAAAGACATGATATTGGATCAACTGCTGATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTAATAAGGGTGTCAGTGTCTATACTAACTACTATTGTTGCAGCTAATAAGTCAGCTATTGAAGACATAAAGAAGAAAGGTCTGCAGTTATCTGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCATCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTATTGGGGGTAGTGTGCTCTCCAAACAGTTATAAGGGTAGGCCAGCATCACTGCCAACACCTCTGACAGCATCACTGATGATAATTGGAGTTTTAGTATCATCATTTGACCATGCCACAAATAATGTGCACTTGGCCGAGATCAGTTATCCCAATGTTCTTCATGGGCTCCTAGATGTTGCAAGAGATAGTAACATTGAAGAATTGATTTCCTGGGCCACTATTCTTGTCAAATGCATACAATATGATGGAAACTGCAGAAGATTTATATCAAGATCAGCTCCATTGGCTCCATTTTCCCGTCTCCTGGAATGTAAGATGAAGCATGCCAGGAGCATAGCACTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCAGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACAAACATTATGAACTCACTCATGCTTTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCAAATAGATACACTAGATAACTCCTCATGTAAGAGTGCATTCAGAGAAGAAGCTATGCAGGTCCTCCTCAAGTTAGTTGCATCCGAACAAAGTTCCACTACACAGAACTTATCTGCATTCATCTTATCAAATCTCGGCGGGACATATTCATGGGCAGGGGAACCATATACAGTTGCATGGTTGGTTAAAAAGGCAGGTGTGACTTCCTCATATCAACGGAATATGATAAAATCTATCCATTGGTTGGATGATTGCTTAGAGGATGCTGGCACAGACTCATGGTGCAGCAAAATTGCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTCATTCTTTAGAGAGGGGTCTAAAGAGTACAACAAGGAAGGTTTCTAGGGACTGTCTAATAGCCATAGCATGGCTTGGGTTTGAAATTGCAAAAAGTCCAGATAGCATCAGATATTCTGCCTGTGAGATCTTACTTAGTGGAGTTGAGCAATTTTTGCATCCTGGACTGGATCTAGAAGAACGGGTTCTAGCATGTCTCTGCATATATAACTATGCTTCTGGTAGAGGGATGACAAAGTTAATTCATTTCTCGGAAGGAGTGAGAGAATCACTAAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGAACACAGCGAATATCTTGTGTTCATACCCAAATTCTCGAGGTTGGATTCAATTTCAGTGGTGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGTTACTCAGATGGTTCTCTGAAGGTCTGGAACATAAAAGGACAATCAGCCACACTTGTATGGGACATGAAGGAGCACAAAAAGGCACTGACATGCTTTTCACTTCTTGAATCAAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTATCAGGGTCTGGCAAGTGGTCCACAAAAAAATGGAGTGTATTGAGGTGATAGAAACCAAGCAACCAATTCGGCATCTAAATACATGTGGGGACATGATATTTGCGATTACCCGCGGCCAGGGGATAAAGGTTTTTGATGCTTCAAGAAAGGTCAAAGAAAACTGCATGAATAAAAGGGTGAAGTGTATGGCAGTGGTTCAGGGGAAGATTTATGCAGGATGCAAGGATTCAAGCATACAGGAGTTATCTACAACAAGCAATCGGGCGCAAGAGATCAAAGCAGCAGCAAAATTTTGGAACCTACAACGGAGACCCATAAATGCAGTTGTCACATATAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGGAAGGTTCAAACTTGAAGGAATGGAAAAGACACCGCACGCCCCAAATGTCACTTAAAACCGGGAAAAGAGAAAAGGTGATGGCAATGGGAATAACAGAAGACTTCATATACCTCAACTGCAGCTCATCAACAAACAGCATCCAGATATGGCTAAGAGGTACACAGCAGAAAGTAGGAAGAATTTCAGCAGGAAGCCGAATCACAAGTATCCTCACAGCCAACGACATTATCTTATGCGGTACAGAAGCGGGGCTGATCAAGGGATGGATCCCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1328

Amino Acids

149.05

Weight (kDa)

8.29

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 35 - 185 3.7e-27 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 503 - 664 2.5e-47 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 668 - 1020 9.5e-58 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_WDR3_1st PF25173 1060 - 1190 2.5e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1060 - 1304 8.6e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1063 - 1136 4.2e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1064 - 1162 1.7e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 675, 1938, 3314
AarI CACCTGC 1 cut(s) 2594
Acc36I ACCTGC 3 cut(s) 457, 2458, 2594
AccB1I GGYRCC 1 cut(s) 352
AccB7I CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
AccII CGCG 1 cut(s) 3450
AciI CCGC 7 cut(s) 284, 2547, 3448, 3450, 3739, 3942, 3953
AcoI YGGCCR 3 cut(s) 302, 2040, 3451
AcuI CTGAAG 2 cut(s) 1041, 3227
AfaI GTAC 7 cut(s) 55, 190, 815, 1365, 2767, 3865, 3946
AfiI CCNNNNNNNGG 6 cut(s) 436, 2212, 2389, 2653, 2808, 3454
AflII CTTAAG 1 cut(s) 1126
AflIII ACRYGT 1 cut(s) 3419
AhdI GACNNNNNGTC 3 cut(s) 97, 733, 1096
AjnI CCWGG 6 cut(s) 2117, 2211, 2234, 2899, 3032, 3453
AjuI GAANNNNNNNTTGG 1 cut(s) 28
Alw21I GWGCWC 5 cut(s) 852, 1816, 1925, 2037, 3264
Alw26I GTCTC 9 cut(s) 75, 79, 502, 505, 689, 720, 2213, 2939, 3640
Alw44I GTGCAC 2 cut(s) 848, 2033
AlwNI CAGNNNCTG 3 cut(s) 2294, 2311, 3345
Ama87I CYCGRG 2 cut(s) 365, 3119
AoxI GGCC 6 cut(s) 302, 489, 1946, 2040, 2121, 3451
ApaLI GTGCAC 2 cut(s) 848, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 3419, 3451
Asp700I GAANNNNTTC 4 cut(s) 1254, 2256, 2922, 3886
AspLEI GCGC 2 cut(s) 566, 3604
AspS9I GGNCC 5 cut(s) 569, 988, 2121, 2470, 3355
AsuC2I CCSGG 1 cut(s) 3766
AsuII TTCGAA 1 cut(s) 442
AsuNHI GCTAGC 1 cut(s) 41
AvaI CYCGRG 2 cut(s) 365, 3119
AvaII GGWCC 4 cut(s) 569, 988, 2470, 3355
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 3 cut(s) 852, 2037, 3157
BalI TGGCCA 1 cut(s) 304
BamHI GGATCC 2 cut(s) 1234, 3972
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 2076
BarI GAAGNNNNNNTAC 2 cut(s) 1675, 1707
BauI CACGAG 1 cut(s) 1221
BbsI GAAGAC 3 cut(s) 1584, 1776, 3810
Bbv12I GWGCWC 5 cut(s) 852, 1816, 1925, 2037, 3264
BbvCI CCTCAGC 1 cut(s) 326
BceAI ACGGC 2 cut(s) 476, 1628
BcgI CGANNNNNNTGC 2 cut(s) 3386, 3420
BciT130I CCWGG 6 cut(s) 2119, 2213, 2236, 2901, 3034, 3455
BciVI GTATCC 2 cut(s) 713, 3923
BclI TGATCA 1 cut(s) 3960
BcnI CCSGG 1 cut(s) 3766
BcoDI GTCTC 9 cut(s) 75, 79, 502, 505, 689, 720, 2213, 2939, 3640
BfrI CTTAAG 1 cut(s) 1126
BfuAI ACCTGC 3 cut(s) 457, 2458, 2594
BfuI GTATCC 2 cut(s) 713, 3923
BglII AGATCT 1 cut(s) 2865
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 7 cut(s) 2119, 2213, 2236, 2901, 3034, 3455, 3766
Bme18I GGWCC 4 cut(s) 569, 988, 2470, 3355
BmeRI GACNNNNNGTC 3 cut(s) 97, 733, 1096
BmeT110I CYCGRG 2 cut(s) 365, 3119
BmgT120I GGNCC 5 cut(s) 569, 988, 2121, 2470, 3355
BmiI GGNNCC 8 cut(s) 354, 1176, 1236, 2075, 2196, 2571, 3635, 3974
BmrFI CCNGG 7 cut(s) 2119, 2213, 2236, 2901, 3034, 3455, 3766
BmrI ACTGGG 2 cut(s) 813, 1561
BmtI GCTAGC 1 cut(s) 45
BmuI ACTGGG 2 cut(s) 813, 1561
BoxI GACNNNNGTC 1 cut(s) 3019
BpiI GAAGAC 3 cut(s) 1584, 1776, 3810
Bpu10I CCTNAGC 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 2 cut(s) 80, 2462
BpuMI CCSGG 1 cut(s) 3766
BsaBI GATNNNNATC 2 cut(s) 951, 2173
BsaI GGTCTC 2 cut(s) 505, 3640
BsaJI CCNNGG 4 cut(s) 2118, 2279, 3448, 3454
BsaXI ACNNNNNCTCC 2 cut(s) 1986, 2016
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 2212, 2389, 2653, 2808, 3454
Bse118I RCCGGY 2 cut(s) 355, 1900
Bse1I ACTGG 6 cut(s) 238, 808, 1556, 2253, 2910, 3330
Bse21I CCTNAGG 1 cut(s) 702
Bse3DI GCAATG 1 cut(s) 3795
Bse8I GATNNNNATC 2 cut(s) 951, 2173
BseBI CCWGG 6 cut(s) 2119, 2213, 2236, 2901, 3034, 3455
BseDI CCNNGG 4 cut(s) 2118, 2279, 3448, 3454
BseJI GATNNNNATC 2 cut(s) 951, 2173
BseLI CCNNNNNNNGG 6 cut(s) 436, 2212, 2389, 2653, 2808, 3454
BseMI GCAATG 1 cut(s) 3795
BseMII CTCAG 8 cut(s) 317, 335, 447, 716, 927, 1397, 1505, 3207
BseNI ACTGG 6 cut(s) 238, 808, 1556, 2253, 2910, 3330
BseRI GAGGAG 6 cut(s) 383, 690, 1715, 2422, 2465, 3059
BseSI GKGCMC 3 cut(s) 852, 2037, 3157
BsgI GTGCAG 3 cut(s) 749, 1170, 2713
Bsh1236I CGCG 1 cut(s) 3450
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 6 cut(s) 304, 491, 1948, 2042, 2123, 3453
BshNI GGYRCC 1 cut(s) 352
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 5 cut(s) 852, 1816, 1925, 2037, 3264
BsiHKCI CYCGRG 2 cut(s) 365, 3119
BsiSI CCGG 3 cut(s) 356, 1901, 3765
BslFI GGGAC 5 cut(s) 2563, 2801, 3263, 3317, 3440
BslI CCNNNNNNNGG 6 cut(s) 436, 2212, 2389, 2653, 2808, 3454
BsmAI GTCTC 9 cut(s) 75, 79, 502, 505, 689, 720, 2213, 2939, 3640
BsmBI CGTCTC 2 cut(s) 75, 2213
BsmFI GGGAC 5 cut(s) 2563, 2801, 3263, 3317, 3440
BsmI GAATGC 4 cut(s) 135, 2274, 2447, 2525
BsnI GGCC 6 cut(s) 304, 491, 1948, 2042, 2123, 3453
Bso31I GGTCTC 2 cut(s) 505, 3640
BsoBI CYCGRG 2 cut(s) 365, 3119
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 7 cut(s) 852, 1816, 1925, 2037, 2076, 3157, 3264
Bsp1407I TGTACA 1 cut(s) 1363
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 7 cut(s) 284, 2547, 3448, 3450, 3739, 3942, 3953
BspANI GGCC 6 cut(s) 304, 491, 1948, 2042, 2123, 3453
BspCNI CTCAG 8 cut(s) 318, 334, 446, 715, 926, 1396, 1506, 3206
BspFNI CGCG 1 cut(s) 3450
BspHI TCATGA 4 cut(s) 865, 1087, 1831, 2260
BspLI GGNNCC 8 cut(s) 354, 1176, 1236, 2075, 2196, 2571, 3635, 3974
BspMAI CTGCAG 4 cut(s) 1173, 1794, 2165, 3827
BspMI ACCTGC 3 cut(s) 457, 2458, 2594
BspOI GCTAGC 1 cut(s) 45
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 352
BspTI CTTAAG 1 cut(s) 1126
BspTNI GGTCTC 2 cut(s) 505, 3640
BsrDI GCAATG 1 cut(s) 3795
BsrFI RCCGGY 2 cut(s) 355, 1900
BsrGI TGTACA 1 cut(s) 1363
BsrI ACTGG 6 cut(s) 238, 808, 1556, 2253, 2910, 3330
BssAI RCCGGY 2 cut(s) 355, 1900
BssECI CCNNGG 4 cut(s) 2118, 2279, 3448, 3454
BssSI CACGAG 1 cut(s) 1221
BssT1I CCWWGG 1 cut(s) 2279
Bst2BI CACGAG 1 cut(s) 1221
Bst2UI CCWGG 6 cut(s) 2119, 2213, 2236, 2901, 3034, 3455
Bst4CI ACNGT 6 cut(s) 401, 1848, 1934, 2581, 2792, 3689
BstAFI CTTAAG 1 cut(s) 1126
BstAPI GCANNNNNTGC 2 cut(s) 2311, 2893
BstAUI TGTACA 1 cut(s) 1363
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 9 cut(s) 43, 357, 1141, 1173, 1457, 1950, 2232, 2677, 3743
BstDSI CCRYGG 1 cut(s) 3448
BstFNI CGCG 1 cut(s) 3450
BstHHI GCGC 2 cut(s) 566, 3604
BstMAI GTCTC 9 cut(s) 75, 79, 502, 505, 689, 720, 2213, 2939, 3640
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 6 cut(s) 352, 1461, 2192, 2311, 2893, 3152
BstNI CCWGG 6 cut(s) 2119, 2213, 2236, 2901, 3034, 3455
BstNSI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
BstPAI GACNNNNGTC 1 cut(s) 3019
BstSCI CCNGG 7 cut(s) 2117, 2211, 2234, 2899, 3032, 3453, 3764
BstSLI GKGCMC 3 cut(s) 852, 2037, 3157
BstUI CGCG 1 cut(s) 3450
BstV2I GAAGAC 3 cut(s) 1584, 1776, 3810
BstX2I RGATCY 5 cut(s) 773, 1234, 2865, 2908, 3972
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 5 cut(s) 773, 1234, 2865, 2908, 3972
Bsu36I CCTNAGG 1 cut(s) 702
BsuI GTATCC 2 cut(s) 713, 3923
BsuRI GGCC 6 cut(s) 304, 491, 1948, 2042, 2123, 3453
BtgI CCRYGG 1 cut(s) 3448
BtsI GCAGTG 3 cut(s) 473, 1955, 3534
BveI ACCTGC 3 cut(s) 457, 2458, 2594
Cac8I GCNNGC 9 cut(s) 43, 357, 1141, 1173, 1457, 1950, 2232, 2677, 3743
CaiI CAGNNNCTG 3 cut(s) 2294, 2311, 3345
CciI TCATGA 4 cut(s) 865, 1087, 1831, 2260
CfoI GCGC 2 cut(s) 566, 3604
Cfr10I RCCGGY 2 cut(s) 355, 1900
Cfr13I GGNCC 5 cut(s) 569, 988, 2121, 2470, 3355
Cfr42I CCGCGG 1 cut(s) 3451
CseI GACGC 1 cut(s) 3024
Csp6I GTAC 7 cut(s) 54, 189, 814, 1364, 2766, 3864, 3945
CspCI CAANNNNNGTGG 6 cut(s) 1041, 1076, 2113, 2148, 3121, 3156
CviQI GTAC 7 cut(s) 54, 189, 814, 1364, 2766, 3864, 3945
DriI GACNNNNNGTC 3 cut(s) 97, 733, 1096
EaeI YGGCCR 3 cut(s) 302, 2040, 3451
Eam1105I GACNNNNNGTC 3 cut(s) 97, 733, 1096
Eco130I CCWWGG 1 cut(s) 2279
Eco24I GRGCYC 1 cut(s) 2076
Eco31I GGTCTC 2 cut(s) 505, 3640
Eco32I GATATC 1 cut(s) 249
Eco47I GGWCC 4 cut(s) 569, 988, 2470, 3355
Eco57I CTGAAG 2 cut(s) 1041, 3227
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 2 cut(s) 365, 3119
EcoO109I RGGNCCY 1 cut(s) 2470
EcoRI GAATTC 3 cut(s) 227, 1297, 1354
EcoRII CCWGG 6 cut(s) 2117, 2211, 2234, 2899, 3032, 3453
EcoRV GATATC 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 2279
EcoT22I ATGCAT 2 cut(s) 137, 2144
EcoT38I GRGCYC 1 cut(s) 2076
ErhI CCWWGG 1 cut(s) 2279
Esp3I CGTCTC 2 cut(s) 75, 2213
FalI AAGNNNNNCTT 4 cut(s) 3162, 3194, 3666, 3698
FaqI GGGAC 5 cut(s) 2563, 2801, 3263, 3317, 3440
FauI CCCGC 3 cut(s) 2540, 3455, 3946
FauNDI CATATG 1 cut(s) 1360
FbaI TGATCA 1 cut(s) 3960
FriOI GRGCYC 1 cut(s) 2076
GlaI GCGC 2 cut(s) 565, 3603
HaeIII GGCC 6 cut(s) 304, 491, 1948, 2042, 2123, 3453
HapII CCGG 3 cut(s) 356, 1901, 3765
HgaI GACGC 1 cut(s) 3024
HhaI GCGC 2 cut(s) 566, 3604
Hin6I GCGC 2 cut(s) 564, 3602
HinP1I GCGC 2 cut(s) 564, 3602
HindIII AAGCTT 1 cut(s) 197
HpaII CCGG 3 cut(s) 356, 1901, 3765
Hpy166II GTNNAC 5 cut(s) 850, 1508, 2035, 3358, 3866
Hpy8I GTNNAC 5 cut(s) 850, 1508, 2035, 3358, 3866
HpyCH4III ACNGT 6 cut(s) 401, 1848, 1934, 2581, 2792, 3689
HpyF10VI GCNNNNNNNGC 6 cut(s) 352, 1461, 2192, 2311, 2893, 3152
HspAI GCGC 2 cut(s) 564, 3602
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 3960
KspI CCGCGG 1 cut(s) 3451
LmnI GCTCC 8 cut(s) 1180, 1187, 2079, 2191, 2200, 2238, 3046, 3259
MfeI CAATTG 3 cut(s) 612, 2366, 3699
MflI RGATCY 5 cut(s) 773, 1234, 2865, 2908, 3972
MhlI GDGCHC 7 cut(s) 852, 1816, 1925, 2037, 2076, 3157, 3264
MlsI TGGCCA 1 cut(s) 304
MluNI TGGCCA 1 cut(s) 304
MmeI TCCRAC 4 cut(s) 765, 2386, 2634, 3106
Mox20I TGGCCA 1 cut(s) 304
Mph1103I ATGCAT 2 cut(s) 137, 2144
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 4 cut(s) 1254, 2256, 2922, 3886
MscI TGGCCA 1 cut(s) 304
MslI CAYNNNNRTG 3 cut(s) 2557, 3244, 3277
Msp20I TGGCCA 1 cut(s) 304
MspA1I CMGCKG 3 cut(s) 325, 386, 3450
MspCI CTTAAG 1 cut(s) 1126
MspI CCGG 3 cut(s) 356, 1901, 3765
MspR9I CCNGG 7 cut(s) 2119, 2213, 2236, 2901, 3034, 3455, 3766
MunI CAATTG 3 cut(s) 612, 2366, 3699
Mva1269I GAATGC 4 cut(s) 135, 2274, 2447, 2525
MvaI CCWGG 6 cut(s) 2119, 2213, 2236, 2901, 3034, 3455
MvnI CGCG 1 cut(s) 3450
MwoI GCNNNNNNNGC 6 cut(s) 352, 1461, 2192, 2311, 2893, 3152
NaeI GCCGGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 3766
NdeI CATATG 1 cut(s) 1360
NgoMIV GCCGGC 1 cut(s) 355
NheI GCTAGC 1 cut(s) 41
NlaIV GGNNCC 8 cut(s) 354, 1176, 1236, 2075, 2196, 2571, 3635, 3974
NmeAIII GCCGAG 2 cut(s) 2068, 2523
NmuCI GTSAC 6 cut(s) 20, 379, 735, 2608, 3664, 3753
NsiI ATGCAT 2 cut(s) 137, 2144
NspI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
NspV TTCGAA 1 cut(s) 442
PaeI GCATGC 1 cut(s) 2234
PaeR7I CTCGAG 2 cut(s) 365, 3119
PagI TCATGA 4 cut(s) 865, 1087, 1831, 2260
PaqCI CACCTGC 1 cut(s) 2594
PciI ACATGT 1 cut(s) 3419
PctI GAATGC 4 cut(s) 135, 2274, 2447, 2525
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 4 cut(s) 1254, 2256, 2922, 3886
PflMI CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
PfoI TCCNGGA 2 cut(s) 2211, 2899
Ple19I CGATCG 1 cut(s) 30
PpuMI RGGWCCY 1 cut(s) 2470
PscI ACATGT 1 cut(s) 3419
PshAI GACNNNNGTC 1 cut(s) 3019
PsiI TTATAA 3 cut(s) 675, 1938, 3314
Psp5II RGGWCCY 1 cut(s) 2470
Psp6I CCWGG 6 cut(s) 2117, 2211, 2234, 2899, 3032, 3453
PspGI CCWGG 6 cut(s) 2117, 2211, 2234, 2899, 3032, 3453
PspN4I GGNNCC 8 cut(s) 354, 1176, 1236, 2075, 2196, 2571, 3635, 3974
PspPI GGNCC 5 cut(s) 569, 988, 2121, 2470, 3355
PspPPI RGGWCCY 1 cut(s) 2470
PspXI VCTCGAGB 1 cut(s) 365
PsrI GAACNNNNNNTAC 2 cut(s) 1464, 1496
PstI CTGCAG 4 cut(s) 1173, 1794, 2165, 3827
PstNI CAGNNNCTG 3 cut(s) 2294, 2311, 3345
PsuI RGATCY 5 cut(s) 773, 1234, 2865, 2908, 3972
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 325, 386
RsaI GTAC 7 cut(s) 55, 190, 815, 1365, 2767, 3865, 3946
RsaNI GTAC 7 cut(s) 54, 189, 814, 1364, 2766, 3864, 3945
RseI CAYNNNNRTG 3 cut(s) 2557, 3244, 3277
SacII CCGCGG 1 cut(s) 3451
Sau96I GGNCC 5 cut(s) 569, 988, 2121, 2470, 3355
ScrFI CCNGG 7 cut(s) 2119, 2213, 2236, 2901, 3034, 3455, 3766
SduI GDGCHC 7 cut(s) 852, 1816, 1925, 2037, 2076, 3157, 3264
Sfr274I CTCGAG 2 cut(s) 365, 3119
Sfr303I CCGCGG 1 cut(s) 3451
SfuI TTCGAA 1 cut(s) 442
SgrBI CCGCGG 1 cut(s) 3451
SinI GGWCC 4 cut(s) 569, 988, 2470, 3355
SlaI CTCGAG 2 cut(s) 365, 3119
SmiMI CAYNNNNRTG 3 cut(s) 2557, 3244, 3277
SmlI CTYRAG 5 cut(s) 95, 365, 1126, 2477, 3119
SmoI CTYRAG 5 cut(s) 95, 365, 1126, 2477, 3119
SphI GCATGC 1 cut(s) 2234
SsiI CCGC 7 cut(s) 284, 2547, 3448, 3450, 3739, 3942, 3953
SspI AATATT 1 cut(s) 1614
StyD4I CCNGG 7 cut(s) 2117, 2211, 2234, 2899, 3032, 3453, 3764
StyI CCWWGG 1 cut(s) 2279
TaaI ACNGT 6 cut(s) 401, 1848, 1934, 2581, 2792, 3689
TaqI TCGA 5 cut(s) 26, 30, 366, 442, 3120
TatI WGTACW 3 cut(s) 813, 1363, 2765
TauI GCSGC 2 cut(s) 287, 3453
TseFI GTSAC 6 cut(s) 20, 379, 735, 2608, 3664, 3753
Tsp45I GTSAC 6 cut(s) 20, 379, 735, 2608, 3664, 3753
TspGWI ACGGA 3 cut(s) 536, 2641, 3658
Van91I CCANNNNNTGG 3 cut(s) 2389, 2653, 2808
Vha464I CTTAAG 1 cut(s) 1126
VneI GTGCAC 2 cut(s) 848, 2033
VpaK11BI GGWCC 4 cut(s) 569, 988, 2470, 3355
XbaI TCTAGA 1 cut(s) 2911
XceI RCATGY 4 cut(s) 2234, 2934, 3282, 3423
XhoI CTCGAG 2 cut(s) 365, 3119
XmnI GAANNNNTTC 4 cut(s) 1254, 2256, 2922, 3886
Zsp2I ATGCAT 2 cut(s) 137, 2144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.