RLG00000007602

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010154
Physical Location & Seq
Reverse (-)
18875775 .. 18882648
6874 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007602

Sequence Viewer

Length: 3990 bp
ATGAACATGAACTTGCTTGGTGACTCGGTGGAAGCTGTAGCTAGCTGTTCTACTACTGCTTTGGTTTCGTCTCATAATCAAGAGAGATTAGACCTCAAGTCCATTCGGGCGGTTGTCATTTCAAGCAATCAATGCATTCTCAAACTCATAGCAAATGCCAAAACTAGAAACTCTATAAGGGTGAGATGTATCTCCAAGCTTCAAAATCAGAAACAGGATTTCTTCGAATTCTCTGACCAGTCTGTCATATCAAATCTTTACTGGGGTATTGACAGCATAGAAGCCGCAATTACCGCCGAATGGCCAGAAGAGAAAGCCGCTCAGCTGAGGAAGGCAGAGCAGATGCTACAAGTGCCCGCGCTACTCGATGAGGATGGAGTCACAGCTGGAATTCCGAACAGTTATATGGTGTGTTGCTCATACTTTTACCTCTCTGTTGTTCGAAAACTCCAAGAGGATGAGTGGCAGGTTGCACTGCATTTCCTACAGGCCGTGCTGGTCTCCCCGAGACTTGTTCAGACAGAATTTGCGCATGACCTTTATGAAAGTCTTTTCCCGACTTGCGGCTGGTCTGAAAGACAAGAAATAAGAGCGAGTAAGAGCTTGGAATCAATTGACAAGGATGAAGCAATAAGGCAGATGGCAAGGATATATAGAGATTGGTTGATGTATTATAAGGTCATGTTATATGGCGCGACTCCTCAGGGTCTAGGTGGAGGATACAGAGGCATTTTGTCCCCTGACAAAGAGTCACTATATTCTCTGCATGGGAAATCCACTAGGTCGGACTGTTCAAATAATATTGAGCATGACCACAGCTTGCACACTCAATGGAATTATGGAAAAGTGCACCCTTTTGATCCTCAAGAAGATATTGTCATCGAAGATGAACTGAAACAATCCATCCACATCTCAGAATCTGAAGAATATGGGAAATCCAAAAATGATTTGAATCAAGCAACAGAGTTTCTTGTTAAAACTAGAGAGCTCCAGAGGAATTCAAGTATTAAAAGACTCCAAGATTTGCTGAATGACTCTCAATCAGACACACCCACTTCAGTTGATTCATGCTCTGATTATTCAGATTATGACATCGAGTCAGAGGTCATTGATGGTGGTGAGTGCTCAACCGGAGATGCAAGAAAAGGCGCAGATTTTTCCAAGAAACTGCAGGCTACTAGCTCCACATCAGATCCAAAGTACGAAGCATTATCTCTTTCACAAGTCTGTCAGGATCCAGTCCCTAACGAAGTCATTCAAGTAAATAACTCTATGATTCCTTCAAGTAGATTCACAAATTCCATCAATGGTTTATTAACTATTTCAGAGCATAGAGATAAAAGATCAAAGACCCATCAGAATTTATATGTACAAAAGGAGTATGCATCTCAGCGGAACTTTAGGATCAACCAAAGGGATCACCAACGAAGTGTTGCAAGGAAAAAACAGAGTTCTCGTAGCCAGAAAAGCTCTATTGAACTTCACGTACATTCCACAAAAGATTCGAAAAGTGAACTACTGAGTATTACTGAAAAAGCAATTTCAAAACTATTTCAGTGGGAAGGATTGGGAAAATGGGATGAAGATTATGCTGTAGAAGTTACAACAATATATCAAATACTATGCAACAAAAAAGGAGAGAAATGTGCTGTCTTGAAGGACATGATATTGGATCAACTGCTCATAGGTATTTCAGCTTCAAAGGAGGAGAAAGTGATAAGGGTATCAGTGTCTATACTAACTACTATTGTTGCAGCTAATAAGTCAGTTATCGAGGACATAAAGAAGAAAGGTCTGCAGTTATCTGATTTGGCAAGTGCTCTAAAAAGAAATGTTCATGAGGCAGCAATACTGTTTTATCTAATGAATCCCTCTCCTACAGAAATTAAGACTTTAGAACTTTTACCGGCATTGTTGGGGGTCGTGTGCTGTCCAAACAGTTATAAGGGTAGGCCAGCATCAGTGCCAACACCTCTGACAGCATCACTAATGATTATTGGAGTTTTAGTATCTTCATTTGACCATGCCACAAATAATGTGCACTTGGCTGAAATCAGTTATCCCAATGTGCTGCATGGGCTCCTAGATGTTGCAAGAGATAGTAACATTGAAGAATTGATCTCCTGGGCCACTATTCTTGTCAAATGCATACAATACGATGGAAACTGCAGAAGATATATATCAAGATTAGCTCCAATGGCTCCATTTTCCCGTCTCCTAGAATGTAAGATGAAGCGTGCCAGGTGCATAGCGCTGGAATTTTTTCATGAAGTTCTTTGCATTCCAAGGTCATCGGCTACTGCCTTGTTGCAGAGGCTGCAAAAAGAAGGAAGCACGAAGATTATGAACTCACTCATGCATTGTGTCCAACAATTGCAACCTGAATACCAACTTTTGGCAGCAAATTTGCTGCTTCATTTGGATACACTAGATAACTCCTCCTGTAACAGTGCATTTACAGAACAAGCTATGCAGGTTCTCCTCAAGTTGGTCTCATCTGAAGAAAGTTCCACCACACAGCACTTATCTGCATTCATCTTATCAAATCTCGGTGGAACATATTCATGGGCAGGGGAACCATATACTGTAGCATGGTTGGTTAAAAAGGCAGGTGTGACTTCCTCGTATCGACGGAATATGATAAAAACTATCCATTGGTTGGATGATTGCTTAGAGGATTCTGGCACGGACTCATGGTGCAGCAAAATTTCCAGAAGCATCATTAACATTGGGAATCCTGTCTTTGATTCTTTAGTGAAGGGTCTAAAGAGCACAACAAGGAAGGTATCTAGGGACTGTCTAATAGCCATAGCATGGCTTGGGTTTGAAATTGCAAAGAGTCCAGATATCATTAGATATTCTGCCTGTGAGATCTTACTTAGTGGAGTTGAGCAATTTTTGCATCCTGGATTGGAGCTAGAAGAACGAGTTCTAGCATGTCTCTGCATATATAACTATGCTTCTGGTAAAGGGATGAAAAAGCTAATTCATTTCTCGGAAGGAGTGAGAGAATCACTGAGACGCTTGTCAAATGTAACCTGGATGGCAGAGGAGCTACATAAAGTAGCTGATTATGTTCTGCCAAACCGATCACAGCGAATTTCTTGTGTTCATACCCAAATTCTCGAGGTTGGAGTCAATTACAGTGGAGCTGTATGTGCCCTCATGTATTACAAAGGATTTCTTCATGGTGGCTACTCAGATGGTTCTGTGAAGGTCTGGAACATAAAAGGTCAGTCAGCCACACTCGTATGGGACATGAAGGAGCACAAAAAGGCAGTTACATGCTTTTCACTACTTGAATCCAGGGACAGCCTTATAAGTGGATCACTGGACAAAACTGTCAGGGTCTGGCAAGTGGTCCGCAAAAAACTGGAGTGCATTGAGGTGATAGAAACCAAGGAACCAATTCGGCATCTGAATACATGCGGGGGCATGATATTTGCAATTACCCGTGGCCATGGAATTAAGGTGATTGATGCATCAAGAAAGGTCAAAGATAATTGCACGAATAAAAAGGTAAAGTGTATGGTGGTGGTTCAGGGGAAGATTTATGCAGGTTGCAAGGATTCAAGCATACAGGAGTTGTCAACAACAAGCAATCGAGCACAAGAGATCAAAGCAGCATCAAAATTTTGGAAGCTGAAACGGAGACCCATAAATGCAGTTGTCATATACAAAGATTGGCTTTACAGTGCAAGTTCAATTGTGCAAGGTTCAAACTTGAAGGAATGGAAAAGACACGGCAAACCCCAAATGTCACTTAAAACCGGGAAAAGAGAAAAGGTGACGGCAATGGGAATAACAGAAGACTTCATATACCTCAACTGCAACTCATCAACAAACAGCATCCAGATATGGCTAAGAGGGACACAGCAGAAAGTGGGCAGAATTTCAGCAGGAAGCCGAATTACGAGCATCCTCACAGCCAATGACATTATCTTGTGTGGTACCGAAGCGGGGCTAATCAAGGGATGGATCCCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

1330

Amino Acids

149.08

Weight (kDa)

8.54

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 35 - 221 3.6e-28 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_2nd PF23654 505 - 666 5.7e-48 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN_C PF23628 670 - 1022 2e-55 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Beta-prop_TEP1_2nd PF25047 1053 - 1121 4.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 1062 - 1125 8.9e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1064 - 1304 1.9e-08 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1065 - 1133 1e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1066 - 1136 6.3e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 675, 1944, 3320
AarI CACCTGC 1 cut(s) 2600
AasI GACNNNNNNGTC 2 cut(s) 242, 3341
Acc16I TGCGCA 1 cut(s) 531
Acc36I ACCTGC 4 cut(s) 457, 2464, 2600, 3548
Acc65I GGTACC 1 cut(s) 3950
AccB1I GGYRCC 1 cut(s) 3950
AccB7I CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
AccBSI CCGCTC 1 cut(s) 320
AccII CGCG 2 cut(s) 359, 695
AcoI YGGCCR 2 cut(s) 302, 3457
AcuI CTGAAG 3 cut(s) 942, 1041, 2520
AfaI GTAC 4 cut(s) 1202, 1371, 1488, 3952
AfeI AGCGCT 1 cut(s) 2253
AfiI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3960
AhdI GACNNNNNGTC 3 cut(s) 97, 748, 1096
AjnI CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3305
Alw21I GWGCWC 8 cut(s) 852, 990, 1127, 1822, 2043, 2774, 3270, 3610
Alw26I GTCTC 8 cut(s) 75, 502, 505, 2219, 2497, 2945, 3013, 3646
Alw44I GTGCAC 2 cut(s) 848, 2039
AlwNI CAGNNNCTG 3 cut(s) 920, 2317, 3351
Ama87I CYCGRG 2 cut(s) 505, 3125
Aor51HI AGCGCT 1 cut(s) 2253
AoxI GGCC 5 cut(s) 302, 489, 1952, 2127, 3457
ApaLI GTGCAC 2 cut(s) 848, 2039
ApeKI GCWGC 8 cut(s) 1754, 1844, 2071, 2317, 2399, 2410, 2700, 3623
Asp700I GAANNNNTTC 5 cut(s) 1254, 2262, 2560, 2928, 3408
Asp718I GGTACC 1 cut(s) 3950
AspLEI GCGC 5 cut(s) 361, 532, 695, 1151, 2254
AspS9I GGNCC 2 cut(s) 2127, 3361
AsuC2I CCSGG 1 cut(s) 3772
AsuHPI GGTGA 7 cut(s) 32, 193, 1130, 1412, 3400, 3484, 3799
AsuII TTCGAA 3 cut(s) 225, 442, 1505
AsuNHI GCTAGC 1 cut(s) 41
AvaI CYCGRG 2 cut(s) 505, 3125
AvaII GGWCC 1 cut(s) 3361
AxyI CCTNAGG 1 cut(s) 702
BaeGI GKGCMC 4 cut(s) 357, 852, 2043, 3163
BalI TGGCCA 2 cut(s) 304, 3459
BamHI GGATCC 2 cut(s) 1234, 3978
BanI GGYRCC 1 cut(s) 3950
BanII GRGCYC 2 cut(s) 990, 2082
BarI GAAGNNNNNNTAC 4 cut(s) 1681, 1713, 3895, 3927
BbsI GAAGAC 1 cut(s) 3816
Bbv12I GWGCWC 8 cut(s) 852, 990, 1127, 1822, 2043, 2774, 3270, 3610
BbvCI CCTCAGC 1 cut(s) 326
BbvI GCAGC 8 cut(s) 1766, 1856, 2058, 2304, 2397, 2411, 2712, 3635
BceAI ACGGC 3 cut(s) 476, 3760, 3807
BciT130I CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3307
BciVI GTATCC 2 cut(s) 713, 2416
BcnI CCSGG 1 cut(s) 3772
BcoDI GTCTC 8 cut(s) 75, 502, 505, 2219, 2497, 2945, 3013, 3646
BfmI CTRYAG 9 cut(s) 36, 485, 1169, 1593, 1796, 1878, 2167, 2586, 3986
BfoI RGCGCY 1 cut(s) 2255
BfuAI ACCTGC 4 cut(s) 457, 2464, 2600, 3548
BfuI GTATCC 2 cut(s) 713, 2416
BglII AGATCT 1 cut(s) 2871
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 6 cut(s) 2125, 2242, 2907, 3040, 3307, 3772
Bme18I GGWCC 1 cut(s) 3361
BmeRI GACNNNNNGTC 3 cut(s) 97, 748, 1096
BmeT110I CYCGRG 2 cut(s) 505, 3125
BmgT120I GGNCC 2 cut(s) 2127, 3361
BmiI GGNNCC 7 cut(s) 1236, 2081, 2202, 2577, 3405, 3952, 3980
BmrFI CCNGG 6 cut(s) 2125, 2242, 2907, 3040, 3307, 3772
BmrI ACTGGG 1 cut(s) 271
BmtI GCTAGC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 271
BoxI GACNNNNGTC 1 cut(s) 3025
BpiI GAAGAC 1 cut(s) 3816
BpmI CTGGAG 2 cut(s) 974, 3395
Bpu10I CCTNAGC 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 3 cut(s) 225, 442, 1505
BpuEI CTTGAG 3 cut(s) 80, 849, 2468
BpuMI CCSGG 1 cut(s) 3772
BsaAI YACGTR 1 cut(s) 1486
BsaBI GATNNNNATC 2 cut(s) 951, 2179
BsaI GGTCTC 3 cut(s) 505, 2497, 3646
BsaJI CCNNGG 6 cut(s) 2124, 2285, 3306, 3399, 3454, 3460
BsaWI WCCGGW 1 cut(s) 1130
BsaXI ACNNNNNCTCC 2 cut(s) 1992, 2022
Bsc4I CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3960
Bse118I RCCGGY 1 cut(s) 1906
Bse1I ACTGG 5 cut(s) 238, 266, 1238, 3336, 3378
Bse21I CCTNAGG 1 cut(s) 702
Bse3DI GCAATG 1 cut(s) 3801
Bse8I GATNNNNATC 2 cut(s) 951, 2179
BseBI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3307
BseDI CCNNGG 6 cut(s) 2124, 2285, 3306, 3399, 3454, 3460
BseJI GATNNNNATC 2 cut(s) 951, 2179
BseLI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3960
BseMI GCAATG 1 cut(s) 3801
BseMII CTCAG 8 cut(s) 317, 335, 716, 927, 1403, 1511, 3008, 3213
BseNI ACTGG 5 cut(s) 238, 266, 1238, 3336, 3378
BseRI GAGGAG 5 cut(s) 690, 1721, 2428, 2471, 3065
BseSI GKGCMC 4 cut(s) 357, 852, 2043, 3163
BseXI GCAGC 8 cut(s) 1766, 1856, 2058, 2304, 2397, 2411, 2712, 3635
BsgI GTGCAG 1 cut(s) 2719
Bsh1236I CGCG 2 cut(s) 359, 695
BshFI GGCC 5 cut(s) 304, 491, 1954, 2129, 3459
BshNI GGYRCC 1 cut(s) 3950
BsiHKAI GWGCWC 8 cut(s) 852, 990, 1127, 1822, 2043, 2774, 3270, 3610
BsiHKCI CYCGRG 2 cut(s) 505, 3125
BsiSI CCGG 3 cut(s) 1131, 1907, 3771
BslFI GGGAC 6 cut(s) 721, 1226, 2807, 3269, 3323, 3883
BslI CCNNNNNNNGG 7 cut(s) 300, 563, 2395, 2488, 2659, 2814, 3960
BsmAI GTCTC 8 cut(s) 75, 502, 505, 2219, 2497, 2945, 3013, 3646
BsmBI CGTCTC 3 cut(s) 75, 2219, 3013
BsmFI GGGAC 6 cut(s) 721, 1226, 2807, 3269, 3323, 3883
BsmI GAATGC 3 cut(s) 135, 2280, 2531
BsnI GGCC 5 cut(s) 304, 491, 1954, 2129, 3459
Bso31I GGTCTC 3 cut(s) 505, 2497, 3646
BsoBI CYCGRG 2 cut(s) 505, 3125
Bsp119I TTCGAA 3 cut(s) 225, 442, 1505
Bsp1407I TGTACA 1 cut(s) 1369
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 3460
BspANI GGCC 5 cut(s) 304, 491, 1954, 2129, 3459
BspCNI CTCAG 8 cut(s) 318, 334, 715, 926, 1402, 1512, 3009, 3212
BspFNI CGCG 2 cut(s) 359, 695
BspHI TCATGA 2 cut(s) 1837, 2266
BspLI GGNNCC 7 cut(s) 1236, 2081, 2202, 2577, 3405, 3952, 3980
BspMAI CTGCAG 3 cut(s) 1173, 1800, 2171
BspMI ACCTGC 4 cut(s) 457, 2464, 2600, 3548
BspOI GCTAGC 1 cut(s) 45
BspT104I TTCGAA 3 cut(s) 225, 442, 1505
BspT107I GGYRCC 1 cut(s) 3950
BspTNI GGTCTC 3 cut(s) 505, 2497, 3646
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 3801
BsrFI RCCGGY 1 cut(s) 1906
BsrGI TGTACA 1 cut(s) 1369
BsrI ACTGG 5 cut(s) 238, 266, 1238, 3336, 3378
BssAI RCCGGY 1 cut(s) 1906
BssECI CCNNGG 6 cut(s) 2124, 2285, 3306, 3399, 3454, 3460
BssT1I CCWWGG 3 cut(s) 2285, 3399, 3460
Bst2UI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3307
Bst6I CTCTTC 1 cut(s) 303
BstAPI GCANNNNNTGC 3 cut(s) 132, 2317, 2899
BstAUI TGTACA 1 cut(s) 1369
BstBAI YACGTR 1 cut(s) 1486
BstBI TTCGAA 3 cut(s) 225, 442, 1505
BstC8I GCNNGC 6 cut(s) 43, 357, 821, 1173, 1956, 2238
BstDSI CCRYGG 2 cut(s) 3454, 3460
BstFNI CGCG 2 cut(s) 359, 695
BstH2I RGCGCY 1 cut(s) 2255
BstHHI GCGC 5 cut(s) 361, 532, 695, 1151, 2254
BstMAI GTCTC 8 cut(s) 75, 502, 505, 2219, 2497, 2945, 3013, 3646
BstMWI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3158
BstNI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3307
BstNSI RCATGY 3 cut(s) 2940, 3288, 3429
BstPAI GACNNNNGTC 1 cut(s) 3025
BstSCI CCNGG 6 cut(s) 2123, 2240, 2905, 3038, 3305, 3770
BstSFI CTRYAG 9 cut(s) 36, 485, 1169, 1593, 1796, 1878, 2167, 2586, 3986
BstSLI GKGCMC 4 cut(s) 357, 852, 2043, 3163
BstUI CGCG 2 cut(s) 359, 695
BstV1I GCAGC 8 cut(s) 1766, 1856, 2058, 2304, 2397, 2411, 2712, 3635
BstV2I GAAGAC 1 cut(s) 3816
BstX2I RGATCY 4 cut(s) 1192, 1234, 2871, 3978
BstYI RGATCY 4 cut(s) 1192, 1234, 2871, 3978
Bsu36I CCTNAGG 1 cut(s) 702
BsuI GTATCC 2 cut(s) 713, 2416
BsuRI GGCC 5 cut(s) 304, 491, 1954, 2129, 3459
BtgI CCRYGG 2 cut(s) 3454, 3460
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 9 cut(s) 473, 1562, 1734, 1968, 2455, 3014, 3151, 3329, 3700
BveI ACCTGC 4 cut(s) 457, 2464, 2600, 3548
Cac8I GCNNGC 6 cut(s) 43, 357, 821, 1173, 1956, 2238
CaiI CAGNNNCTG 3 cut(s) 920, 2317, 3351
CciI TCATGA 2 cut(s) 1837, 2266
CfoI GCGC 5 cut(s) 361, 532, 695, 1151, 2254
Cfr10I RCCGGY 1 cut(s) 1906
Cfr13I GGNCC 2 cut(s) 2127, 3361
CseI GACGC 1 cut(s) 3030
Csp6I GTAC 4 cut(s) 1201, 1370, 1487, 3951
CspCI CAANNNNNGTGG 6 cut(s) 1042, 1077, 2119, 2154, 3127, 3162
CviQI GTAC 4 cut(s) 1201, 1370, 1487, 3951
DrdI GACNNNNNNGTC 2 cut(s) 242, 3341
DriI GACNNNNNGTC 3 cut(s) 97, 748, 1096
DseDI GACNNNNNNGTC 2 cut(s) 242, 3341
EaeI YGGCCR 2 cut(s) 302, 3457
Eam1104I CTCTTC 1 cut(s) 303
Eam1105I GACNNNNNGTC 3 cut(s) 97, 748, 1096
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 3 cut(s) 2285, 3399, 3460
Eco24I GRGCYC 2 cut(s) 990, 2082
Eco31I GGTCTC 3 cut(s) 505, 2497, 3646
Eco32I GATATC 1 cut(s) 2848
Eco47I GGWCC 1 cut(s) 3361
Eco47III AGCGCT 1 cut(s) 2253
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 3 cut(s) 942, 1041, 2520
Eco81I CCTNAGG 1 cut(s) 702
Eco88I CYCGRG 2 cut(s) 505, 3125
EcoICRI GAGCTC 1 cut(s) 988
EcoRI GAATTC 3 cut(s) 227, 390, 997
EcoRII CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3305
EcoRV GATATC 1 cut(s) 2848
EcoT14I CCWWGG 3 cut(s) 2285, 3399, 3460
EcoT22I ATGCAT 5 cut(s) 137, 1387, 2150, 2361, 3484
EcoT38I GRGCYC 2 cut(s) 990, 2082
ErhI CCWWGG 3 cut(s) 2285, 3399, 3460
Esp3I CGTCTC 3 cut(s) 75, 2219, 3013
FalI AAGNNNNNCTT 4 cut(s) 3168, 3200, 3672, 3704
FaqI GGGAC 6 cut(s) 721, 1226, 2807, 3269, 3323, 3883
FauI CCCGC 3 cut(s) 364, 3422, 3952
FriOI GRGCYC 2 cut(s) 990, 2082
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 5 cut(s) 360, 531, 694, 1150, 2253
GsuI CTGGAG 2 cut(s) 974, 3395
HaeII RGCGCY 1 cut(s) 2255
HaeIII GGCC 5 cut(s) 304, 491, 1954, 2129, 3459
HapII CCGG 3 cut(s) 1131, 1907, 3771
HgaI GACGC 1 cut(s) 3030
HhaI GCGC 5 cut(s) 361, 532, 695, 1151, 2254
Hin6I GCGC 5 cut(s) 359, 530, 693, 1149, 2252
HinP1I GCGC 5 cut(s) 359, 530, 693, 1149, 2252
HincII GTYRAC 1 cut(s) 3591
HindII GTYRAC 1 cut(s) 3591
HindIII AAGCTT 1 cut(s) 197
HpaII CCGG 3 cut(s) 1131, 1907, 3771
HphI GGTGA 7 cut(s) 32, 193, 1130, 1412, 3400, 3484, 3799
Hpy166II GTNNAC 4 cut(s) 850, 1514, 2041, 3591
Hpy8I GTNNAC 4 cut(s) 850, 1514, 2041, 3591
Hpy99I CGWCG 1 cut(s) 2634
HpyCH4IV ACGT 1 cut(s) 1485
HpyF10VI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3158
HpySE526I ACGT 1 cut(s) 1485
HspAI GCGC 5 cut(s) 359, 530, 693, 1149, 2252
KpnI GGTACC 1 cut(s) 3954
LmnI GCTCC 9 cut(s) 993, 1187, 2085, 2197, 2206, 2914, 3052, 3149, 3265
Lsp1109I GCAGC 8 cut(s) 1766, 1856, 2058, 2304, 2397, 2411, 2712, 3635
MaeII ACGT 1 cut(s) 1485
MbiI CCGCTC 1 cut(s) 320
MfeI CAATTG 3 cut(s) 612, 2372, 3705
MflI RGATCY 4 cut(s) 1192, 1234, 2871, 3978
MlsI TGGCCA 2 cut(s) 304, 3459
MluNI TGGCCA 2 cut(s) 304, 3459
MmeI TCCRAC 4 cut(s) 765, 2392, 2640, 3112
Mox20I TGGCCA 2 cut(s) 304, 3459
Mph1103I ATGCAT 5 cut(s) 137, 1387, 2150, 2361, 3484
MroXI GAANNNNTTC 5 cut(s) 1254, 2262, 2560, 2928, 3408
MscI TGGCCA 2 cut(s) 304, 3459
MseI TTAA 8 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 3468, 3765
MslI CAYNNNNRTG 3 cut(s) 2563, 3250, 3386
Msp20I TGGCCA 2 cut(s) 304, 3459
MspA1I CMGCKG 3 cut(s) 325, 386, 1393
MspI CCGG 3 cut(s) 1131, 1907, 3771
MspR9I CCNGG 6 cut(s) 2125, 2242, 2907, 3040, 3307, 3772
MunI CAATTG 3 cut(s) 612, 2372, 3705
Mva1269I GAATGC 3 cut(s) 135, 2280, 2531
MvaI CCWGG 5 cut(s) 2125, 2242, 2907, 3040, 3307
MvnI CGCG 2 cut(s) 359, 695
MwoI GCNNNNNNNGC 9 cut(s) 132, 293, 352, 1467, 2077, 2198, 2317, 2899, 3158
NciI CCSGG 1 cut(s) 3772
NcoI CCATGG 1 cut(s) 3460
NheI GCTAGC 1 cut(s) 41
NlaIV GGNNCC 7 cut(s) 1236, 2081, 2202, 2577, 3405, 3952, 3980
NmuCI GTSAC 6 cut(s) 20, 379, 750, 2614, 3759, 3787
NsbI TGCGCA 1 cut(s) 531
NsiI ATGCAT 5 cut(s) 137, 1387, 2150, 2361, 3484
NspI RCATGY 3 cut(s) 2940, 3288, 3429
NspV TTCGAA 3 cut(s) 225, 442, 1505
PaeR7I CTCGAG 1 cut(s) 3125
PagI TCATGA 2 cut(s) 1837, 2266
PaqCI CACCTGC 1 cut(s) 2600
PctI GAATGC 3 cut(s) 135, 2280, 2531
PdmI GAANNNNTTC 5 cut(s) 1254, 2262, 2560, 2928, 3408
PflMI CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
PfoI TCCNGGA 1 cut(s) 2905
Ppu21I YACGTR 1 cut(s) 1486
PshAI GACNNNNGTC 1 cut(s) 3025
PsiI TTATAA 3 cut(s) 675, 1944, 3320
Psp124BI GAGCTC 1 cut(s) 990
Psp6I CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3305
PspGI CCWGG 5 cut(s) 2123, 2240, 2905, 3038, 3305
PspN4I GGNNCC 7 cut(s) 1236, 2081, 2202, 2577, 3405, 3952, 3980
PspPI GGNCC 2 cut(s) 2127, 3361
PsrI GAACNNNNNNTAC 2 cut(s) 1470, 1502
PstI CTGCAG 3 cut(s) 1173, 1800, 2171
PstNI CAGNNNCTG 3 cut(s) 920, 2317, 3351
PsuI RGATCY 4 cut(s) 1192, 1234, 2871, 3978
PvuII CAGCTG 2 cut(s) 325, 386
RsaI GTAC 4 cut(s) 1202, 1371, 1488, 3952
RsaNI GTAC 4 cut(s) 1201, 1370, 1487, 3951
RseI CAYNNNNRTG 3 cut(s) 2563, 3250, 3386
SacI GAGCTC 1 cut(s) 990
SaqAI TTAA 8 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 3468, 3765
Sau96I GGNCC 2 cut(s) 2127, 3361
ScrFI CCNGG 6 cut(s) 2125, 2242, 2907, 3040, 3307, 3772
SfcI CTRYAG 9 cut(s) 36, 485, 1169, 1593, 1796, 1878, 2167, 2586, 3986
Sfr274I CTCGAG 1 cut(s) 3125
SfuI TTCGAA 3 cut(s) 225, 442, 1505
SinI GGWCC 1 cut(s) 3361
SlaI CTCGAG 1 cut(s) 3125
SmiMI CAYNNNNRTG 3 cut(s) 2563, 3250, 3386
SmlI CTYRAG 4 cut(s) 95, 864, 2483, 3125
SmoI CTYRAG 4 cut(s) 95, 864, 2483, 3125
SspI AATATT 1 cut(s) 802
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 6 cut(s) 2123, 2240, 2905, 3038, 3305, 3770
StyI CCWWGG 3 cut(s) 2285, 3399, 3460
TaiI ACGT 1 cut(s) 1488
TatI WGTACW 1 cut(s) 1369
TauI GCSGC 3 cut(s) 287, 320, 567
Tru1I TTAA 8 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 3468, 3765
Tru9I TTAA 8 cut(s) 975, 1008, 1316, 1887, 2601, 2724, 3468, 3765
TscAI CASTG 9 cut(s) 480, 1562, 1734, 1968, 2455, 3021, 3151, 3336, 3700
TseFI GTSAC 6 cut(s) 20, 379, 750, 2614, 3759, 3787
TseI GCWGC 8 cut(s) 1754, 1844, 2071, 2317, 2399, 2410, 2700, 3623
Tsp45I GTSAC 6 cut(s) 20, 379, 750, 2614, 3759, 3787
TspGWI ACGGA 3 cut(s) 2647, 2702, 3664
TspRI CASTG 9 cut(s) 480, 1562, 1734, 1968, 2455, 3021, 3151, 3336, 3700
Van91I CCANNNNNTGG 3 cut(s) 2395, 2659, 2814
VneI GTGCAC 2 cut(s) 848, 2039
VpaK11BI GGWCC 1 cut(s) 3361
XceI RCATGY 3 cut(s) 2940, 3288, 3429
XhoI CTCGAG 1 cut(s) 3125
XmnI GAANNNNTTC 5 cut(s) 1254, 2262, 2560, 2928, 3408
Zsp2I ATGCAT 5 cut(s) 137, 1387, 2150, 2361, 3484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.