AT3G08505

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009491
Physical Location & Seq
Reverse (-)
2580077 .. 2582248
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G08505.1

Sequence Viewer

Length: 972 bp
ATGTCCGATAGGATACTGTGCAAGTTCTTTGTCCATGGCTCGTGCTTGAAAGGCGAAAATTGCGAATTTTCCCATGACTCTAAGGATCCGCCCAATAATGTTTGCACATTCTATCAGAAACGAATATGTTTGTACGGAAGTAGATGTAGATACGATCATGTCAGAGCTGCTTCCAATCTTCCTCTTAGCTCAGATTCTGAGTCTTTAGATCGCTCTATATCCACCACCCCCTCTAGGCATTTGCAACAACAAGGCGATAATAATGACGGTGACAAATCCTCTAACGTTTACTGTATCCACCCTAGAGAGTACCCGATCTGCTCTTTTGCTGCAGCTGGTGATTGCCCTCGAGGTAACCAGTGTCCACATATGCATGGTGACCTCTGCAATACTTGTGGCAAGAAGTGTTTGCATCCTTTTAGACCCGAGGAACGAGAAGAGCATACGAAAGAATGTGAGAAGAAGCAGAAGCACATTGAGGCTTTGAAACAAAGTCAAGATATTGAGTGTAGTGTGTGTTTGGATCGTATCTTGTCAAAGGCTACTCCAGGGGAAAGGAAGTTTGGGCTGTTGACTGAGTGTGATCATCCGTTTTGTATACAATGTATTCGTAATTGGCGTAGCAGTGCTCCTGTTTCGGGAATGGATGTCAACAGTACGTTGAGAGCTTGTCCTATATGTCGCAAACTCTCTTATTTTGTTGTCCCTAGTGTGGTCTGGTACTCTTCTCCTGAGGAGAAGAAGGAAATTATCGACATATACAAGGCTAAACTCAGATCAATCGACTGTAAGCACTTCAACTTTGGAAATGGAAACTGCCCATTTGGAGCAAGTTGTTTCTATAAGCATGCATATTCTGATGGTCACTTGGAAGAGGTTGTTTTACGGCATCTTGGTTCTCAAGAAGGAGAAACTGTAATAACAGACAGTATCAGGCTATCAGAGTTCCTTGGTGGCCTGCAGATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-CCCH zf-RING_2

Protein Analysis

323

Amino Acids

36.56

Weight (kDa)

7.16

Isoelectric Point (pI)

64.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_4 PF18044 6 - 26 4.5e-06 CCCH-type zinc finger
zf_CCCH_4 PF18345 7 - 25 4.7e-06 Zinc finger domain
zf-RING_2 PF13639 169 - 228 9.6e-06 Ring finger domain
zf-C3HC4 PF00097 170 - 227 7.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 348
AccI GTMKAC 1 cut(s) 598
AciI CCGC 1 cut(s) 89
AclI AACGTT 1 cut(s) 285
AclWI GGATC 3 cut(s) 80, 93, 531
AcsI RAATTY 1 cut(s) 65
AfaI GTAC 4 cut(s) 134, 311, 658, 722
AfiI CCNNNNNNNGG 3 cut(s) 234, 638, 712
AgsI TTSAA 3 cut(s) 49, 487, 799
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
AluBI AGCT 4 cut(s) 167, 189, 335, 668
AluI AGCT 4 cut(s) 167, 189, 335, 668
Alw21I GWGCWC 1 cut(s) 631
AlwI GGATC 3 cut(s) 80, 93, 531
AlwNI CAGNNNCTG 1 cut(s) 197
Ama87I CYCGRG 2 cut(s) 348, 425
AoxI GGCC 1 cut(s) 955
ApeKI GCWGC 3 cut(s) 167, 329, 332
ApoI RAATTY 1 cut(s) 65
AsuHPI GGTGA 3 cut(s) 281, 350, 389
AvaI CYCGRG 2 cut(s) 348, 425
AxyI CCTNAGG 1 cut(s) 732
BamHI GGATCC 1 cut(s) 85
BauI CACGAG 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 3 cut(s) 154, 316, 344
BccI CCATC 1 cut(s) 854
BceAI ACGGC 1 cut(s) 902
BciT130I CCWGG 1 cut(s) 549
BciVI GTATCC 2 cut(s) 6, 305
BclI TGATCA 1 cut(s) 583
BfaI CTAG 3 cut(s) 234, 303, 708
BfmI CTRYAG 2 cut(s) 330, 959
BfuI GTATCC 2 cut(s) 6, 305
BisI GCNGC 3 cut(s) 168, 330, 333
BlsI GCNGC 3 cut(s) 169, 331, 334
Bme1390I CCNGG 1 cut(s) 549
BmeT110I CYCGRG 2 cut(s) 348, 425
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 421, 898
BpmI CTGGAG 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 885
BsaJI CCNNGG 4 cut(s) 34, 426, 548, 949
BsaXI ACNNNNNCTCC 2 cut(s) 900, 930
Bsc4I CCNNNNNNNGG 3 cut(s) 234, 638, 712
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 732
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 4 cut(s) 34, 426, 548, 949
BseGI GGATG 3 cut(s) 412, 586, 652
BseLI CCNNNNNNNGG 3 cut(s) 234, 638, 712
BseMII CTCAG 5 cut(s) 189, 204, 567, 723, 787
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 3 cut(s) 154, 316, 344
BshFI GGCC 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 631
BsiHKCI CYCGRG 2 cut(s) 348, 425
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 3 cut(s) 234, 638, 712
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 1 cut(s) 957
BsoBI CYCGRG 2 cut(s) 348, 425
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 1 cut(s) 89
BspANI GGCC 1 cut(s) 957
BspCNI CTCAG 5 cut(s) 190, 203, 568, 724, 786
BspLI GGNNCC 1 cut(s) 87
BspMAI CTGCAG 2 cut(s) 334, 963
BspPI GGATC 3 cut(s) 80, 93, 531
BspQI GCTCTTC 1 cut(s) 432
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 4 cut(s) 34, 426, 548, 949
BssMI GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
BssNAI GTATAC 1 cut(s) 599
BssSI CACGAG 1 cut(s) 40
BssT1I CCWWGG 2 cut(s) 34, 949
Bst1107I GTATAC 1 cut(s) 599
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 7 cut(s) 18, 269, 293, 656, 788, 916, 929
Bst6I CTCTTC 3 cut(s) 432, 730, 867
BstC8I GCNNGC 2 cut(s) 849, 959
BstDEI CTNAG 7 cut(s) 81, 185, 190, 198, 576, 732, 773
BstDSI CCRYGG 1 cut(s) 34
BstEII GGTNACC 2 cut(s) 353, 377
BstF5I GGATG 3 cut(s) 412, 586, 652
BstKTI GATC 7 cut(s) 88, 157, 211, 318, 526, 586, 779
BstMBI GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
BstMWI GCNNNNNNNGC 2 cut(s) 51, 60
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 851
BstPI GGTNACC 2 cut(s) 353, 377
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 2 cut(s) 330, 959
BstV1I GCAGC 3 cut(s) 154, 316, 344
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
BstZ17I GTATAC 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 732
BsuI GTATCC 2 cut(s) 6, 305
BsuRI GGCC 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 3 cut(s) 412, 586, 652
BtsI GCAGTG 1 cut(s) 631
BtsIMutI CAGTG 2 cut(s) 365, 631
Cac8I GCNNGC 2 cut(s) 849, 959
CaiI CAGNNNCTG 1 cut(s) 197
Csp6I GTAC 4 cut(s) 133, 310, 657, 721
CspCI CAANNNNNGTGG 2 cut(s) 376, 411
CviAII CATG 5 cut(s) 35, 74, 158, 374, 848
CviQI GTAC 4 cut(s) 133, 310, 657, 721
DdeI CTNAG 7 cut(s) 81, 185, 190, 198, 576, 732, 773
DpnI GATC 7 cut(s) 87, 156, 210, 317, 525, 585, 778
DpnII GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
Eam1104I CTCTTC 3 cut(s) 432, 730, 867
EarI CTCTTC 3 cut(s) 432, 730, 867
EciI GGCGGA 1 cut(s) 78
Eco130I CCWWGG 2 cut(s) 34, 949
Eco81I CCTNAGG 1 cut(s) 732
Eco88I CYCGRG 2 cut(s) 348, 425
Eco91I GGTNACC 2 cut(s) 353, 377
EcoO65I GGTNACC 2 cut(s) 353, 377
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 34, 949
EcoT22I ATGCAT 2 cut(s) 375, 853
ErhI CCWWGG 2 cut(s) 34, 949
FaeI CATG 5 cut(s) 38, 77, 161, 377, 851
FaqI GGGAC 1 cut(s) 689
FatI CATG 5 cut(s) 34, 73, 157, 373, 847
FauNDI CATATG 1 cut(s) 369
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 168, 330, 333
FokI GGATG 3 cut(s) 399, 573, 659
Fsp4HI GCNGC 3 cut(s) 168, 330, 333
FspBI CTAG 3 cut(s) 234, 303, 708
GluI GCNGC 3 cut(s) 168, 330, 333
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 1 cut(s) 957
Hin1II CATG 5 cut(s) 38, 77, 161, 377, 851
HincII GTYRAC 2 cut(s) 573, 652
HindII GTYRAC 2 cut(s) 573, 652
HinfI GANTC 3 cut(s) 77, 194, 200
HphI GGTGA 3 cut(s) 281, 350, 389
Hpy166II GTNNAC 5 cut(s) 289, 365, 573, 599, 652
Hpy188I TCNGA 8 cut(s) 7, 117, 164, 193, 199, 776, 859, 943
Hpy188III TCNNGA 4 cut(s) 497, 639, 731, 902
Hpy8I GTNNAC 5 cut(s) 289, 365, 573, 599, 652
HpyAV CCTTC 2 cut(s) 736, 899
HpyCH4III ACNGT 7 cut(s) 18, 269, 293, 656, 788, 916, 929
HpyCH4IV ACGT 2 cut(s) 285, 659
HpyCH4V TGCA 9 cut(s) 21, 105, 244, 332, 373, 387, 412, 851, 961
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 60
HpyF3I CTNAG 7 cut(s) 81, 185, 190, 198, 576, 732, 773
HpySE526I ACGT 2 cut(s) 285, 659
Hsp92II CATG 5 cut(s) 38, 77, 161, 377, 851
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 634, 827
LpnPI CCDG 8 cut(s) 321, 371, 534, 561, 645, 703, 744, 919
Lsp1109I GCAGC 3 cut(s) 154, 316, 344
LweI GCATC 2 cut(s) 421, 898
MaeI CTAG 3 cut(s) 234, 303, 708
MaeII ACGT 2 cut(s) 285, 659
MaeIII GTNAC 4 cut(s) 269, 353, 377, 863
MalI GATC 7 cut(s) 87, 156, 210, 317, 525, 585, 778
MboI GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
MboII GAAGA 6 cut(s) 170, 449, 472, 717, 751, 884
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 4 cut(s) 58, 65, 613, 747
MlyI GAGTC 2 cut(s) 71, 209
Mph1103I ATGCAT 2 cut(s) 375, 853
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 1 cut(s) 549
MvaI CCWGG 1 cut(s) 549
MwoI GCNNNNNNNGC 2 cut(s) 51, 60
NcoI CCATGG 1 cut(s) 34
NdeI CATATG 1 cut(s) 369
NdeII GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
NlaIII CATG 5 cut(s) 38, 77, 161, 377, 851
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 3 cut(s) 269, 377, 863
NsiI ATGCAT 2 cut(s) 375, 853
NspI RCATGY 1 cut(s) 851
PaeI GCATGC 1 cut(s) 851
PaeR7I CTCGAG 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 3 cut(s) 169, 331, 334
PleI GAGTC 2 cut(s) 71, 208
PpsI GAGTC 2 cut(s) 71, 208
Psp1406I AACGTT 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 547
PspEI GGTNACC 2 cut(s) 353, 377
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 348
PstI CTGCAG 2 cut(s) 334, 963
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 4 cut(s) 134, 311, 658, 722
RsaNI GTAC 4 cut(s) 133, 310, 657, 721
RseI CAYNNNNRTG 1 cut(s) 372
SapI GCTCTTC 1 cut(s) 432
SatI GCNGC 3 cut(s) 168, 330, 333
Sau3AI GATC 7 cut(s) 85, 154, 208, 315, 523, 583, 776
SchI GAGTC 2 cut(s) 71, 209
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 1 cut(s) 631
SetI ASST 9 cut(s) 169, 191, 288, 337, 355, 384, 662, 670, 879
SfaNI GCATC 2 cut(s) 421, 898
SfcI CTRYAG 2 cut(s) 330, 959
Sfr274I CTCGAG 1 cut(s) 348
SlaI CTCGAG 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 348, 900
SmoI CTYRAG 2 cut(s) 348, 900
SphI GCATGC 1 cut(s) 851
Sse9I AATT 4 cut(s) 58, 65, 613, 747
SsiI CCGC 1 cut(s) 89
SspMI CTAG 3 cut(s) 234, 303, 708
StyD4I CCNGG 1 cut(s) 547
StyI CCWWGG 2 cut(s) 34, 949
TaaI ACNGT 7 cut(s) 18, 269, 293, 656, 788, 916, 929
TaiI ACGT 2 cut(s) 288, 662
TaqI TCGA 3 cut(s) 349, 753, 783
TasI AATT 4 cut(s) 58, 65, 613, 747
TfiI GAWTC 1 cut(s) 194
TscAI CASTG 2 cut(s) 365, 631
TseFI GTSAC 3 cut(s) 269, 377, 863
TseI GCWGC 3 cut(s) 167, 329, 332
Tsp45I GTSAC 3 cut(s) 269, 377, 863
TspGWI ACGGA 2 cut(s) 150, 579
TspRI CASTG 2 cut(s) 365, 631
XapI RAATTY 1 cut(s) 65
XceI RCATGY 1 cut(s) 851
XhoI CTCGAG 1 cut(s) 348
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 3 cut(s) 234, 303, 708
Zsp2I ATGCAT 2 cut(s) 375, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.