AT3G09180

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004370
Physical Location & Seq
Forward (+)
2819040 .. 2821441
2402 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09180.1

Sequence Viewer

Length: 1209 bp
ATGCAGACGCTACACCAGTCCCAACTTCTCCAGAATCCGGCCGAGGCGGCGAACAATCAGTCTGAATCGGACGCGCCGCCGAAACAGGTGGCTCAGGCCATGGAACGACTCAATCAAGCGGCTAGGGTTATCGCCGATATCCGCCTCGGCGCTGACCGTATCCTTGAAGCTATGTTCGTGGCTTCGCAACCTCGTCACACAGACATGCCTCTTCAGCTCTTTCTCAGAGAAGACGCCTCCATGCGTCAACATCTTCAGGATCTTCGCTTAATAGGGAAGAAATTGGAAGAATCTGGAGTGTTAACTGAATCACTTAGGTCAAGAAGTAACTCTTGGGGTCTTCACATGCCTTTGGTTTGTCCAGATGGTGCAGTCGTTGCTTATGCTTGGAAAAGACAGCTTGCTGGCCAAGCTGGTGCTTCTGCTGTTGATAGAACCAGGTTAGCTCTCAAGGCCTTCACGGATCAGAAAAGACGATTCTTCCCTCATATTGATGATGGATTAAAGATGGAACCAAGCTCAAAAAAACATCGTGCCTCCCATTTGCTATTAGAAAATGGAAGAGAGGAGCCGGTTGATTACAAAACATTGCCAGATATACAATCGCGTTTAGAGAAGCTGGTTCCGAGTGTGAAGGTGTCTACTTATGGACGATTGAACTGGCTTAAAAGAGCTAACTCTTTACCTGGGTCAGGTAGTGATGATCCTACAGAAGCATCGAAACCTATTTTCCAGAGTTCTAGCAAGCTGAGATCAGGGTTACAGACTGAAGTTGTCGACAAGATTGCTGTTATTGAGTTATCATTTCCTTCTCTATTCAGAGCTATAGTCTCGTTGAGTCCAGCGGGTTCTGTTGATCCAGATGCAGTTGCTTTCTTCTCCCCAGATGAGGGAGGCAGCTACTTACATGCGAGAGGCTTCTCGGTTTATCATGTTTACAAACACATAACGGAACATGCTGCTACAGCCTTGCAATATTTCCTCGGCTTTGGTACTGGAACAGCTCTCTACTCTCTTCTGCTATGGATATGCAGTTTTGAATCCGTATTTAGCAAACCTTGCACTAAATGTGGAAGGCTATTAGCAATGGATAAAAAGTCTGCGTTAATCTTACCTCCTTTACACCGCGCTTACCAAGAATTGCCTCTTGCATTAAACCTGGACGTTTGTGAAGCTTACCACTCCAGTTGCTCTCAAGATGATACCTAG

Protein Analysis

402

Amino Acids

44.51

Weight (kDa)

8.14

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med27 PF11571 308 - 397 1.5e-20 Mediator complex subunit 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 641, 777
AccII CGCG 3 cut(s) 74, 607, 1128
AciI CCGC 6 cut(s) 47, 77, 119, 142, 845, 1126
AclWI GGATC 4 cut(s) 267, 471, 698, 851
AcoI YGGCCR 2 cut(s) 39, 406
AcuI CTGAAG 3 cut(s) 197, 239, 789
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 994
AfiI CCNNNNNNNGG 4 cut(s) 37, 692, 889, 890
AgsI TTSAA 3 cut(s) 167, 658, 1040
AjnI CCWGG 3 cut(s) 437, 685, 1158
AjuI GAANNNNNNNTTGG 2 cut(s) 316, 348
Alw26I GTCTC 1 cut(s) 835
AlwI GGATC 4 cut(s) 267, 471, 698, 851
AoxI GGCC 4 cut(s) 39, 96, 406, 453
ApeKI GCWGC 2 cut(s) 897, 959
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 76, 152, 1130
BalI TGGCCA 1 cut(s) 408
BbsI GAAGAC 2 cut(s) 237, 332
BbvI GCAGC 2 cut(s) 909, 946
BccI CCATC 3 cut(s) 359, 491, 502
BcgI CGANNNNNNTGC 2 cut(s) 767, 801
BciT130I CCWGG 3 cut(s) 439, 687, 1160
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 835
BfaI CTAG 3 cut(s) 123, 741, 1207
BfmI CTRYAG 3 cut(s) 708, 825, 963
BfoI RGCGCY 1 cut(s) 153
BfuI GTATCC 1 cut(s) 170
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 5 cut(s) 48, 77, 120, 898, 960
BlsI GCNGC 5 cut(s) 49, 78, 121, 899, 961
Bme1390I CCNGG 3 cut(s) 439, 687, 1160
BmiI GGNNCC 3 cut(s) 513, 570, 624
BmrFI CCNGG 3 cut(s) 439, 687, 1160
BmsI GCATC 2 cut(s) 725, 853
BpiI GAAGAC 2 cut(s) 237, 332
BpmI CTGGAG 3 cut(s) 14, 315, 1168
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 434, 1179
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 5 cut(s) 42, 99, 145, 686, 982
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 692, 889, 890
Bse118I RCCGGY 1 cut(s) 571
Bse1I ACTGG 4 cut(s) 16, 665, 1000, 1185
Bse3DI GCAATG 2 cut(s) 587, 1092
BseBI CCWGG 3 cut(s) 439, 687, 1160
BseDI CCNNGG 5 cut(s) 42, 99, 145, 686, 982
BseLI CCNNNNNNNGG 4 cut(s) 37, 692, 889, 890
BseMI GCAATG 2 cut(s) 587, 1092
BseMII CTCAG 3 cut(s) 107, 238, 740
BseNI ACTGG 4 cut(s) 16, 665, 1000, 1185
BseRI GAGGAG 1 cut(s) 581
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 909, 946
BsgI GTGCAG 1 cut(s) 390
Bsh1236I CGCG 3 cut(s) 74, 607, 1128
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 41, 98, 408, 455
BsiEI CGRYCG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 38, 572
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 4 cut(s) 37, 692, 889, 890
BsmAI GTCTC 1 cut(s) 835
BsmFI GGGAC 1 cut(s) 4
BsnI GGCC 4 cut(s) 41, 98, 408, 455
Bsp143I GATC 5 cut(s) 259, 463, 703, 752, 856
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 6 cut(s) 47, 77, 119, 142, 845, 1126
BspANI GGCC 4 cut(s) 41, 98, 408, 455
BspCNI CTCAG 3 cut(s) 106, 237, 741
BspFNI CGCG 3 cut(s) 74, 607, 1128
BspLI GGNNCC 3 cut(s) 513, 570, 624
BspPI GGATC 4 cut(s) 267, 471, 698, 851
BsrDI GCAATG 2 cut(s) 587, 1092
BsrFI RCCGGY 1 cut(s) 571
BsrI ACTGG 4 cut(s) 16, 665, 1000, 1185
BssAI RCCGGY 1 cut(s) 571
BssECI CCNNGG 5 cut(s) 42, 99, 145, 686, 982
BssMI GATC 5 cut(s) 259, 463, 703, 752, 856
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 3 cut(s) 439, 687, 1160
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 3 cut(s) 216, 556, 1020
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 2 cut(s) 377, 1059
BstC8I GCNNGC 3 cut(s) 402, 406, 746
BstDEI CTNAG 4 cut(s) 93, 224, 314, 749
BstDSI CCRYGG 1 cut(s) 99
BstENI CCTNNNNNAGG 1 cut(s) 690
BstFNI CGCG 3 cut(s) 74, 607, 1128
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 3 cut(s) 76, 152, 1130
BstKTI GATC 5 cut(s) 262, 466, 706, 755, 859
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 5 cut(s) 259, 463, 703, 752, 856
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 7 cut(s) 47, 214, 377, 410, 452, 965, 1059
BstNI CCWGG 3 cut(s) 439, 687, 1160
BstNSI RCATGY 4 cut(s) 208, 349, 911, 959
BstSCI CCNGG 3 cut(s) 437, 685, 1158
BstSFI CTRYAG 3 cut(s) 708, 825, 963
BstUI CGCG 3 cut(s) 74, 607, 1128
BstV1I GCAGC 2 cut(s) 909, 946
BstV2I GAAGAC 2 cut(s) 237, 332
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BstZI CGGCCG 1 cut(s) 39
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 4 cut(s) 41, 98, 408, 455
BtgI CCRYGG 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 402, 406, 746
CfoI GCGC 3 cut(s) 76, 152, 1130
Cfr10I RCCGGY 1 cut(s) 571
CseI GACGC 4 cut(s) 16, 80, 233, 242
CsiI ACCWGGT 1 cut(s) 437
Csp6I GTAC 1 cut(s) 993
CspCI CAANNNNNGTGG 2 cut(s) 1169, 1204
CviAII CATG 7 cut(s) 100, 205, 241, 346, 908, 932, 956
CviQI GTAC 1 cut(s) 993
DdeI CTNAG 4 cut(s) 93, 224, 314, 749
DpnI GATC 5 cut(s) 261, 465, 705, 754, 858
DpnII GATC 5 cut(s) 259, 463, 703, 752, 856
EaeI YGGCCR 2 cut(s) 39, 406
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 216, 556, 1020
EarI CTCTTC 3 cut(s) 216, 556, 1020
EciI GGCGGA 1 cut(s) 131
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 455
Eco32I GATATC 1 cut(s) 139
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 3 cut(s) 197, 239, 789
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoRII CCWGG 3 cut(s) 437, 685, 1158
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 7 cut(s) 103, 208, 244, 349, 911, 935, 959
FalI AAGNNNNNCTT 2 cut(s) 316, 348
FaqI GGGAC 1 cut(s) 4
FatI CATG 7 cut(s) 99, 204, 240, 345, 907, 931, 955
FauI CCCGC 1 cut(s) 838
FblI GTMKAC 2 cut(s) 641, 777
Fnu4HI GCNGC 5 cut(s) 48, 77, 120, 898, 960
Fsp4HI GCNGC 5 cut(s) 48, 77, 120, 898, 960
FspBI CTAG 3 cut(s) 123, 741, 1207
GlaI GCGC 3 cut(s) 75, 151, 1129
GluI GCNGC 5 cut(s) 48, 77, 120, 898, 960
GsuI CTGGAG 3 cut(s) 14, 315, 1168
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 4 cut(s) 41, 98, 408, 455
HapII CCGG 2 cut(s) 38, 572
HgaI GACGC 4 cut(s) 16, 80, 233, 242
HhaI GCGC 3 cut(s) 76, 152, 1130
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 7 cut(s) 103, 208, 244, 349, 911, 935, 959
Hin6I GCGC 3 cut(s) 74, 150, 1128
HinP1I GCGC 3 cut(s) 74, 150, 1128
HincII GTYRAC 3 cut(s) 248, 303, 778
HindII GTYRAC 3 cut(s) 248, 303, 778
HindIII AAGCTT 1 cut(s) 1173
HinfI GANTC 8 cut(s) 34, 65, 108, 290, 308, 477, 838, 1040
HpaI GTTAAC 1 cut(s) 303
HpaII CCGG 2 cut(s) 38, 572
Hpy166II GTNNAC 5 cut(s) 248, 303, 642, 778, 937
Hpy188I TCNGA 6 cut(s) 64, 70, 227, 468, 627, 821
Hpy188III TCNNGA 8 cut(s) 31, 257, 294, 321, 362, 733, 860, 1196
Hpy8I GTNNAC 5 cut(s) 248, 303, 642, 778, 937
HpyAV CCTTC 4 cut(s) 466, 628, 819, 1068
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 1164
HpyCH4V TGCA 7 cut(s) 4, 371, 866, 973, 1032, 1062, 1151
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 214, 377, 410, 452, 965, 1059
HpyF3I CTNAG 4 cut(s) 93, 224, 314, 749
HpySE526I ACGT 1 cut(s) 1164
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 7 cut(s) 103, 208, 244, 349, 911, 935, 959
HspAI GCGC 3 cut(s) 74, 150, 1128
KspAI GTTAAC 1 cut(s) 303
Kzo9I GATC 5 cut(s) 259, 463, 703, 752, 856
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 2 cut(s) 909, 946
LweI GCATC 2 cut(s) 725, 853
MabI ACCWGGT 1 cut(s) 437
MaeI CTAG 3 cut(s) 123, 741, 1207
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 3 cut(s) 194, 326, 759
MalI GATC 5 cut(s) 261, 465, 705, 754, 858
MboI GATC 5 cut(s) 259, 463, 703, 752, 856
MflI RGATCY 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 2 cut(s) 281, 1139
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 2 cut(s) 102, 847
Mox20I TGGCCA 1 cut(s) 408
MscI TGGCCA 1 cut(s) 408
MseI TTAA 6 cut(s) 269, 302, 503, 666, 1106, 1154
MslI CAYNNNNRTG 2 cut(s) 203, 492
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 38, 572
MspR9I CCNGG 3 cut(s) 439, 687, 1160
MvaI CCWGG 3 cut(s) 439, 687, 1160
MvnI CGCG 3 cut(s) 74, 607, 1128
MwoI GCNNNNNNNGC 7 cut(s) 47, 214, 377, 410, 452, 965, 1059
NcoI CCATGG 1 cut(s) 99
NdeII GATC 5 cut(s) 259, 463, 703, 752, 856
NlaIII CATG 7 cut(s) 103, 208, 244, 349, 911, 935, 959
NlaIV GGNNCC 3 cut(s) 513, 570, 624
NmeAIII GCCGAG 3 cut(s) 67, 126, 963
NmuCI GTSAC 1 cut(s) 194
NspI RCATGY 4 cut(s) 208, 349, 911, 959
PceI AGGCCT 1 cut(s) 455
PfeI GAWTC 6 cut(s) 34, 65, 290, 308, 477, 1040
PkrI GCNGC 5 cut(s) 49, 78, 121, 899, 961
PleI GAGTC 2 cut(s) 102, 846
PpsI GAGTC 2 cut(s) 102, 846
Psp6I CCWGG 3 cut(s) 437, 685, 1158
PspGI CCWGG 3 cut(s) 437, 685, 1158
PspN4I GGNNCC 3 cut(s) 513, 570, 624
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 994
RsaNI GTAC 1 cut(s) 993
RseI CAYNNNNRTG 2 cut(s) 203, 492
SalI GTCGAC 1 cut(s) 776
SaqAI TTAA 6 cut(s) 269, 302, 503, 666, 1106, 1154
SatI GCNGC 5 cut(s) 48, 77, 120, 898, 960
Sau3AI GATC 5 cut(s) 259, 463, 703, 752, 856
SchI GAGTC 2 cut(s) 102, 847
ScrFI CCNGG 3 cut(s) 439, 687, 1160
SexAI ACCWGGT 1 cut(s) 437
SfaNI GCATC 2 cut(s) 725, 853
SfcI CTRYAG 3 cut(s) 708, 825, 963
SmiMI CAYNNNNRTG 2 cut(s) 203, 492
SmlI CTYRAG 2 cut(s) 449, 1194
SmoI CTYRAG 2 cut(s) 449, 1194
Sse9I AATT 2 cut(s) 281, 1139
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 6 cut(s) 47, 77, 119, 142, 845, 1126
SspI AATATT 1 cut(s) 977
SspMI CTAG 3 cut(s) 123, 741, 1207
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 437, 685, 1158
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 1167
TaqI TCGA 2 cut(s) 719, 777
TasI AATT 2 cut(s) 281, 1139
TauI GCSGC 3 cut(s) 50, 79, 122
TfiI GAWTC 6 cut(s) 34, 65, 290, 308, 477, 1040
Tru1I TTAA 6 cut(s) 269, 302, 503, 666, 1106, 1154
Tru9I TTAA 6 cut(s) 269, 302, 503, 666, 1106, 1154
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 2 cut(s) 897, 959
Tsp45I GTSAC 1 cut(s) 194
TspGWI ACGGA 3 cut(s) 476, 965, 1033
XagI CCTNNNNNAGG 1 cut(s) 690
XceI RCATGY 4 cut(s) 208, 349, 911, 959
XmiI GTMKAC 2 cut(s) 641, 777
XspI CTAG 3 cut(s) 123, 741, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.