Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0001389

Physical Location & Seq

Genomic Coordinates Forward (+)

15236955 .. 15239787

Gene Structure 2833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0093700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGATGTTGTGCAAGAAGTTATGGTCAGACAGATTTAGTAATCTTCCGGACGACGTTGCTCATCATATTCTGTCCTTCCTCGGTGTCAGAGATCTCGCTTCTGTAGGGGCTGTGTCCAAAAGGTGTAGAGAATTTTACCTCTCAGTACCATCAGTGAATATTGATGCAAGTTGTTATCTCCATGCAGTTCCATTAGTGAATCTTGATGCAAGTTGTTATCTAAAAACCAGCAGCAAGTTAGGAGCGCTGATGTGTTTCTGGGATAGATACATGTTTCAGCGTGGTGTTAATAAGATGCAACGCTTTTACATTAATTGGACTTTCTTCACACGCGGCATTGCGAATAAAGACTCTGTGGAGCAATATCGAATAAACTCTTGGATTCATAACGCAGTAAGGTGTAATGTTGAAGAGCTTAACCTTAATTTCACAGCATGTGGTGGATTCACGTTTGTGCTGCCATCTTGCCTATATCAGTCACAATCTTTCAAGTCTCTAGTATTAAATTTAAAATGCTTGGATGAGATGACTCTTGAAGCACCACCAGTATCTCTTTCCTGCAATCTCCGTTACTTGCAGTTGACTTCTGTTAGAATAGCAGATGAAAAGTTTTGCAAGTGGATCTCCTCTAGCTGCAAATGCATTAAGGAATTACAGCTTATTCAAGTTAAAGGGATTAAAAATATTACCATTCAAAGCTCGTCTTTGGAGTCATTTACTTTTGTTGGTATGGACTTGGTCGACCTTAATATCTCCGGTCAGAAGCTTAGAAGTATTGAAATAAAGTGGAACTTTTATTCTTCACCTGGGTACTGCTCATTGAATATATCTGCTCCAAATCTGGAAGATTTAACATGGGCGGGCAAGGTCTTGAATAGCCAAAATCTGGGCAAATTACTGAATTTAAAAAGAGCTTTCATTGCTCTTGAGCCTACGGTAGATGACAATTTATCTGAGGTCTTTTCTAGTATATGTAGGACTAAAGATCTTGGTATAAATGAGAACACCATGAAGGCTATGTTCAAAGATTGTTTCATGCAAGCACCATTAGATGACATTCGTCGCCTGGGTCTTCAACTTCCGAGCTTCAGTAATGACATAGTTCCAATAACGGCCTCTCTTCTCAGAAGAACTGGTTATCTGAATAGCTTGGTACTGATGAGTTCTAAACCTTCTAGATCGGACTCTAAATCTATATCTTCATCTGGATTTGATATGGGATACTGGAAATTGCAAAACCTCACTTTCGTTTACCAGCTGGAGGATTTGCGCATAGACCTCTCCAGCGGGTCTAATGAAATTGAGTTTGCAAAGTACATTCTCGAGAATGCTCCGAATTTGAAGAAGATCGTGATCCGCCATCATCCGGGATCCCAACAATCAAATATCGTAGTTGGGAAGGTGACCAGATGCAAGATCATTTCGAGAGCCATTATTGTGATCCAGGAAATTCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med27

Protein Analysis

485

Amino Acids

54.95

Weight (kDa)

8.97

Isoelectric Point (pI)

51.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	12 - 44	1.7e-06	F-box domain
F-box-like	PF12937	12 - 44	6.3e-07	F-box-like
LRR_At1g61320_AtMIF1	PF23622	113 - 480	5.8e-14	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	125 - 251	1.2e-08	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001389)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04680 AT4G04680 AT5G06350 AT5G06350 AT5G27010
fragaria_vesca	FvH4_6g20280 FvH4_6g20280 FvH4_6g20280 FvH4_6g20280 FvH4_6g20280 FvH4_6g20280 FvH4_6g20280 FvH4_6g20280
malus_domestica	MD10G1053900.v1.1 MD12G1040000.v1.1
prunus_persica	Prupe.7G095000_v2.0.a1 Prupe.7G095000_v2.0.a1 Prupe.7G095000_v2.0.a1 Prupe.7G095000_v2.0.a1 Prupe.7G095000_v2.0.a1
pyrus_communis	pycom01g00990 pycom12g03390 pycom12g03410 pycom14g03330
rosa_chinensis	RchiOBHm_Chr1g0331061 RchiOBHm_Chr1g0331071 RchiOBHm_Chr1g0331091 RchiOBHm_Chr3g0475871
rosa_laevigata	RLG00000023804 RLG00000029754 RLG00000029755 RLG00000029756
rosa_multiflora	Rmu_sc0000539.1_g000093 Rmu_sc0002515.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319460 Rroxscaffold_4G00319470 Rroxscaffold_4G00319490 Rroxscaffold_6G00405770
rosa_rugosa	Rorug01G0093700 Rorug01G0093900 Rorug03G0151800
rosa_samantha	Rh1AG113900 Rh1AG114000 Rh1CG110500 Rh1CG110600 Rh1DG119300 Rh3AG201900 Rh3BG232400 Rh3CG227000 Rh3DG227500
rosa_wichuraiana	Rw1G009270 Rw3G018380 Rw3G018410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1271
AccB7I	CCANNNNNTGG	1 cut(s)	886
AccI	GTMKAC	1 cut(s)	741
AccII	CGCG	1 cut(s)	333
AccIII	TCCGGA	1 cut(s)	46
AciI	CCGC	4 cut(s)	333, 860, 1287, 1357
AclWI	GGATC	5 cut(s)	629, 1348, 1365, 1378, 1435
AcsI	RAATTY	5 cut(s)	131, 505, 901, 1336, 1449
AcuI	CTGAAG	1 cut(s)	1072
AfaI	GTAC	4 cut(s)	147, 812, 1155, 1316
AfeI	AGCGCT	1 cut(s)	246
AfiI	CCNNNNNNNGG	3 cut(s)	886, 1261, 1366
AflIII	ACRYGT	1 cut(s)	270
AjnI	CCWGG	3 cut(s)	805, 1065, 1443
AjuI	GAANNNNNNNTTGG	2 cut(s)	361, 393
AleI	CACNNNNGTG	1 cut(s)	452
AluBI	AGCT	9 cut(s)	415, 633, 658, 699, 766, 914, 1086, 1149, 1258
AluI	AGCT	9 cut(s)	415, 633, 658, 699, 766, 914, 1086, 1149, 1258
Alw26I	GTCTC	1 cut(s)	498
AlwI	GGATC	5 cut(s)	629, 1348, 1365, 1378, 1435
Ama87I	CYCGRG	1 cut(s)	1322
Aor13HI	TCCGGA	1 cut(s)	46
Aor51HI	AGCGCT	1 cut(s)	246
AoxI	GGCC	1 cut(s)	1113
ApeKI	GCWGC	3 cut(s)	231, 457, 633
ApoI	RAATTY	5 cut(s)	131, 505, 901, 1336, 1449
AseI	ATTAAT	1 cut(s)	312
AspLEI	GCGC	2 cut(s)	247, 1272
AsuC2I	CCSGG	1 cut(s)	1368
AsuHPI	GGTGA	2 cut(s)	795, 1414
AvaI	CYCGRG	1 cut(s)	1322
BamHI	GGATCC	1 cut(s)	1370
BbsI	GAAGAC	1 cut(s)	1064
BbvI	GCAGC	3 cut(s)	243, 444, 620
BccI	CCATC	3 cut(s)	157, 469, 1368
BceAI	ACGGC	1 cut(s)	1128
BciT130I	CCWGG	3 cut(s)	807, 1067, 1445
BciVI	GTATCC	1 cut(s)	1214
BcnI	CCSGG	1 cut(s)	1368
BcoDI	GTCTC	1 cut(s)	498
BfaI	CTAG	4 cut(s)	497, 630, 966, 1176
BfmI	CTRYAG	1 cut(s)	102
BfoI	RGCGCY	1 cut(s)	248
BfuI	GTATCC	1 cut(s)	1214
BglII	AGATCT	2 cut(s)	91, 985
BisI	GCNGC	4 cut(s)	232, 334, 458, 634
BlsI	GCNGC	4 cut(s)	233, 335, 459, 635
Bme1390I	CCNGG	4 cut(s)	807, 1067, 1368, 1445
BmeT110I	CYCGRG	1 cut(s)	1322
BmiI	GGNNCC	1 cut(s)	1372
BmrFI	CCNGG	4 cut(s)	807, 1067, 1368, 1445
BmsI	GCATC	4 cut(s)	154, 196, 285, 1400
BoxI	GACNNNNGTC	1 cut(s)	1059
BpiI	GAAGAC	1 cut(s)	1064
BpmI	CTGGAG	2 cut(s)	1267, 1280
BpuEI	CTTGAG	1 cut(s)	947
BpuMI	CCSGG	1 cut(s)	1368
BsaBI	GATNNNNATC	1 cut(s)	1352
BsaJI	CCNNGG	3 cut(s)	79, 806, 1066
BsaWI	WCCGGW	2 cut(s)	46, 755
Bsc4I	CCNNNNNNNGG	3 cut(s)	886, 1261, 1366
Bse1I	ACTGG	3 cut(s)	545, 1138, 1229
Bse3DI	GCAATG	2 cut(s)	336, 918
Bse8I	GATNNNNATC	1 cut(s)	1352
BseAI	TCCGGA	1 cut(s)	46
BseBI	CCWGG	3 cut(s)	807, 1067, 1445
BseDI	CCNNGG	3 cut(s)	79, 806, 1066
BseGI	GGATG	2 cut(s)	526, 1363
BseJI	GATNNNNATC	1 cut(s)	1352
BseLI	CCNNNNNNNGG	3 cut(s)	886, 1261, 1366
BseMI	GCAATG	2 cut(s)	336, 918
BseMII	CTCAG	3 cut(s)	156, 945, 1138
BseNI	ACTGG	3 cut(s)	545, 1138, 1229
BseRI	GAGGAG	1 cut(s)	616
BseXI	GCAGC	3 cut(s)	243, 444, 620
Bsh1236I	CGCG	1 cut(s)	333
BshFI	GGCC	1 cut(s)	1115
BsiHKCI	CYCGRG	1 cut(s)	1322
BsiSI	CCGG	3 cut(s)	47, 756, 1367
BslI	CCNNNNNNNGG	3 cut(s)	886, 1261, 1366
BsmAI	GTCTC	1 cut(s)	498
BsmI	GAATGC	1 cut(s)	1333
BsnI	GGCC	1 cut(s)	1115
BsoBI	CYCGRG	1 cut(s)	1322
Bsp13I	TCCGGA	1 cut(s)	46
Bsp143I	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
BspACI	CCGC	4 cut(s)	333, 860, 1287, 1357
BspANI	GGCC	1 cut(s)	1115
BspCNI	CTCAG	3 cut(s)	155, 946, 1137
BspEI	TCCGGA	1 cut(s)	46
BspFNI	CGCG	1 cut(s)	333
BspLI	GGNNCC	1 cut(s)	1372
BspPI	GGATC	5 cut(s)	629, 1348, 1365, 1378, 1435
BspQI	GCTCTTC	1 cut(s)	405
BsrDI	GCAATG	2 cut(s)	336, 918
BsrI	ACTGG	3 cut(s)	545, 1138, 1229
BssECI	CCNNGG	3 cut(s)	79, 806, 1066
BssMI	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
Bst2UI	CCWGG	3 cut(s)	807, 1067, 1445
Bst4CI	ACNGT	1 cut(s)	937
Bst6I	CTCTTC	2 cut(s)	405, 1125
BstC8I	GCNNGC	2 cut(s)	862, 1041
BstDEI	CTNAG	4 cut(s)	142, 767, 954, 1124
BstEII	GGTNACC	1 cut(s)	1402
BstF5I	GGATG	2 cut(s)	526, 1363
BstFNI	CGCG	1 cut(s)	333
BstH2I	RGCGCY	1 cut(s)	248
BstHHI	GCGC	2 cut(s)	247, 1272
BstKTI	GATC	9 cut(s)	94, 624, 988, 1181, 1350, 1356, 1373, 1419, 1443
BstMAI	GTCTC	1 cut(s)	498
BstMBI	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
BstMWI	GCNNNNNNNGC	2 cut(s)	639, 920
BstNI	CCWGG	3 cut(s)	807, 1067, 1445
BstNSI	RCATGY	2 cut(s)	274, 438
BstPAI	GACNNNNGTC	1 cut(s)	1059
BstPI	GGTNACC	1 cut(s)	1402
BstSCI	CCNGG	4 cut(s)	805, 1065, 1366, 1443
BstSFI	CTRYAG	1 cut(s)	102
BstUI	CGCG	1 cut(s)	333
BstV1I	GCAGC	3 cut(s)	243, 444, 620
BstV2I	GAAGAC	1 cut(s)	1064
BstX2I	RGATCY	4 cut(s)	91, 621, 985, 1370
BstYI	RGATCY	4 cut(s)	91, 621, 985, 1370
BsuI	GTATCC	1 cut(s)	1214
BsuRI	GGCC	1 cut(s)	1115
BtsCI	GGATG	2 cut(s)	526, 1363
BtsIMutI	CAGTG	1 cut(s)	159
Cac8I	GCNNGC	2 cut(s)	862, 1041
CfoI	GCGC	2 cut(s)	247, 1272
Csp6I	GTAC	4 cut(s)	146, 811, 1154, 1315
CviAII	CATG	6 cut(s)	182, 271, 435, 855, 1009, 1036
CviQI	GTAC	4 cut(s)	146, 811, 1154, 1315
DdeI	CTNAG	4 cut(s)	142, 767, 954, 1124
DpnI	GATC	9 cut(s)	93, 623, 987, 1180, 1349, 1355, 1372, 1418, 1442
DpnII	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
DraI	TTTAAA	2 cut(s)	510, 906
Eam1104I	CTCTTC	2 cut(s)	405, 1125
EarI	CTCTTC	2 cut(s)	405, 1125
EciI	GGCGGA	1 cut(s)	1346
Eco47III	AGCGCT	1 cut(s)	246
Eco57I	CTGAAG	1 cut(s)	1072
Eco88I	CYCGRG	1 cut(s)	1322
Eco91I	GGTNACC	1 cut(s)	1402
EcoO65I	GGTNACC	1 cut(s)	1402
EcoRII	CCWGG	3 cut(s)	805, 1065, 1443
EcoT22I	ATGCAT	1 cut(s)	644
FaeI	CATG	6 cut(s)	185, 274, 438, 858, 1012, 1039
FalI	AAGNNNNNCTT	4 cut(s)	688, 720, 776, 808
FatI	CATG	6 cut(s)	181, 270, 434, 854, 1008, 1035
FauI	CCCGC	2 cut(s)	853, 1280
FblI	GTMKAC	1 cut(s)	741
Fnu4HI	GCNGC	4 cut(s)	232, 334, 458, 634
FokI	GGATG	2 cut(s)	533, 1350
Fsp4HI	GCNGC	4 cut(s)	232, 334, 458, 634
FspBI	CTAG	4 cut(s)	497, 630, 966, 1176
FspI	TGCGCA	1 cut(s)	1271
GlaI	GCGC	2 cut(s)	246, 1271
GluI	GCNGC	4 cut(s)	232, 334, 458, 634
GsuI	CTGGAG	2 cut(s)	1267, 1280
HaeII	RGCGCY	1 cut(s)	248
HaeIII	GGCC	1 cut(s)	1115
HapII	CCGG	3 cut(s)	47, 756, 1367
HhaI	GCGC	2 cut(s)	247, 1272
Hin1II	CATG	6 cut(s)	185, 274, 438, 858, 1012, 1039
Hin6I	GCGC	2 cut(s)	245, 1270
HinP1I	GCGC	2 cut(s)	245, 1270
HincII	GTYRAC	2 cut(s)	582, 742
HindII	GTYRAC	2 cut(s)	582, 742
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	7 cut(s)	199, 350, 382, 444, 529, 710, 1184
HpaII	CCGG	3 cut(s)	47, 756, 1367
HphI	GGTGA	2 cut(s)	795, 1414
Hpy166II	GTNNAC	3 cut(s)	582, 742, 1252
Hpy188I	TCNGA	9 cut(s)	28, 89, 762, 955, 1083, 1127, 1143, 1183, 1335
Hpy8I	GTNNAC	3 cut(s)	582, 742, 1252
Hpy99I	CGWCG	2 cut(s)	56, 1065
HpyAV	CCTTC	4 cut(s)	85, 1006, 1182, 1393
HpyCH4III	ACNGT	1 cut(s)	937
HpyCH4IV	ACGT	2 cut(s)	54, 449
HpyF10VI	GCNNNNNNNGC	2 cut(s)	639, 920
HpyF3I	CTNAG	4 cut(s)	142, 767, 954, 1124
HpySE526I	ACGT	2 cut(s)	54, 449
Hsp92II	CATG	6 cut(s)	185, 274, 438, 858, 1012, 1039
HspAI	GCGC	2 cut(s)	245, 1270
Kpn2I	TCCGGA	1 cut(s)	46
Kzo9I	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
LguI	GCTCTTC	1 cut(s)	405
LmnI	GCTCC	4 cut(s)	242, 358, 838, 1336
Lsp1109I	GCAGC	3 cut(s)	243, 444, 620
LweI	GCATC	4 cut(s)	154, 196, 285, 1400
MaeI	CTAG	4 cut(s)	497, 630, 966, 1176
MaeII	ACGT	2 cut(s)	54, 449
MaeIII	GTNAC	3 cut(s)	477, 569, 1402
MalI	GATC	9 cut(s)	93, 623, 987, 1180, 1349, 1355, 1372, 1418, 1442
MboI	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
MflI	RGATCY	4 cut(s)	91, 621, 985, 1370
MlyI	GAGTC	4 cut(s)	344, 523, 719, 1178
MnlI	CCTC	8 cut(s)	89, 149, 637, 949, 1126, 1250, 1255, 1289
Mph1103I	ATGCAT	1 cut(s)	644
MroI	TCCGGA	1 cut(s)	46
MslI	CAYNNNNRTG	2 cut(s)	452, 1436
MspA1I	CMGCKG	2 cut(s)	1258, 1287
MspI	CCGG	3 cut(s)	47, 756, 1367
MspR9I	CCNGG	4 cut(s)	807, 1067, 1368, 1445
Mva1269I	GAATGC	1 cut(s)	1333
MvaI	CCWGG	3 cut(s)	807, 1067, 1445
MvnI	CGCG	1 cut(s)	333
MwoI	GCNNNNNNNGC	2 cut(s)	639, 920
NciI	CCSGG	1 cut(s)	1368
NdeII	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
NlaIII	CATG	6 cut(s)	185, 274, 438, 858, 1012, 1039
NlaIV	GGNNCC	1 cut(s)	1372
NmuCI	GTSAC	2 cut(s)	477, 1402
NsbI	TGCGCA	1 cut(s)	1271
NsiI	ATGCAT	1 cut(s)	644
NspI	RCATGY	2 cut(s)	274, 438
OliI	CACNNNNGTG	1 cut(s)	452
PaeR7I	CTCGAG	1 cut(s)	1322
PciI	ACATGT	1 cut(s)	270
PciSI	GCTCTTC	1 cut(s)	405
PctI	GAATGC	1 cut(s)	1333
PfeI	GAWTC	3 cut(s)	199, 382, 444
PflFI	GACNNNGTC	1 cut(s)	737
PflMI	CCANNNNNTGG	1 cut(s)	886
PfoI	TCCNGGA	2 cut(s)	1366, 1443
PkrI	GCNGC	4 cut(s)	233, 335, 459, 635
PleI	GAGTC	4 cut(s)	344, 523, 718, 1178
PpsI	GAGTC	4 cut(s)	344, 523, 718, 1178
PscI	ACATGT	1 cut(s)	270
PshAI	GACNNNNGTC	1 cut(s)	1059
PshBI	ATTAAT	1 cut(s)	312
Psp6I	CCWGG	3 cut(s)	805, 1065, 1443
PspEI	GGTNACC	1 cut(s)	1402
PspGI	CCWGG	3 cut(s)	805, 1065, 1443
PspN4I	GGNNCC	1 cut(s)	1372
PsuI	RGATCY	4 cut(s)	91, 621, 985, 1370
PsyI	GACNNNGTC	1 cut(s)	737
PvuII	CAGCTG	1 cut(s)	1258
RsaI	GTAC	4 cut(s)	147, 812, 1155, 1316
RsaNI	GTAC	4 cut(s)	146, 811, 1154, 1315
RseI	CAYNNNNRTG	2 cut(s)	452, 1436
SalI	GTCGAC	1 cut(s)	740
SapI	GCTCTTC	1 cut(s)	405
SatI	GCNGC	4 cut(s)	232, 334, 458, 634
Sau3AI	GATC	9 cut(s)	91, 621, 985, 1178, 1347, 1353, 1370, 1416, 1440
SchI	GAGTC	4 cut(s)	344, 523, 719, 1178
ScrFI	CCNGG	4 cut(s)	807, 1067, 1368, 1445
SfaNI	GCATC	4 cut(s)	154, 196, 285, 1400
SfcI	CTRYAG	1 cut(s)	102
Sfr274I	CTCGAG	1 cut(s)	1322
SlaI	CTCGAG	1 cut(s)	1322
SmiMI	CAYNNNNRTG	2 cut(s)	452, 1436
SmlI	CTYRAG	2 cut(s)	926, 1322
SmoI	CTYRAG	2 cut(s)	926, 1322
SsiI	CCGC	4 cut(s)	333, 860, 1287, 1357
SspI	AATATT	2 cut(s)	160, 685
SspMI	CTAG	4 cut(s)	497, 630, 966, 1176
StyD4I	CCNGG	4 cut(s)	805, 1065, 1366, 1443
TaaI	ACNGT	1 cut(s)	937
TaiI	ACGT	2 cut(s)	57, 452
TaqI	TCGA	4 cut(s)	367, 741, 1323, 1424
TatI	WGTACW	1 cut(s)	1314
TauI	GCSGC	1 cut(s)	336
TfiI	GAWTC	3 cut(s)	199, 382, 444
TscAI	CASTG	1 cut(s)	159
TseFI	GTSAC	2 cut(s)	477, 1402
TseI	GCWGC	3 cut(s)	231, 457, 633
Tsp45I	GTSAC	2 cut(s)	477, 1402
TspDTI	ATGAA	7 cut(s)	374, 618, 907, 1024, 1025, 1191, 1311
TspGWI	ACGGA	1 cut(s)	557
TspRI	CASTG	1 cut(s)	159
Tth111I	GACNNNGTC	1 cut(s)	737
Van91I	CCANNNNNTGG	1 cut(s)	886
VspI	ATTAAT	1 cut(s)	312
XapI	RAATTY	5 cut(s)	131, 505, 901, 1336, 1449
XbaI	TCTAGA	1 cut(s)	1175
XceI	RCATGY	2 cut(s)	274, 438
XhoI	CTCGAG	1 cut(s)	1322
XmiI	GTMKAC	1 cut(s)	741
XspI	CTAG	4 cut(s)	497, 630, 966, 1176
Zsp2I	ATGCAT	1 cut(s)	644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.