Rmu_sc0000539.1_g000093

Testis-expressed sequence 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0001389
Physical Location & Seq
Reverse (-)
415780 .. 426468
10689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000539.1_g000093.1.cds

Sequence Viewer

Length: 2661 bp
atggcaggttctaaaaatgcctcaaagaagaagcaaaagcgcggtatcgacttcaagaaaataaagcgaaagattggcagaaaattgccaccagcacagaatgctactaatactgagattaaatctaaagcaattgttctccctgagcagagtgtggcatccgagaaggcaggattagcagtgaacaagaaaggcttgactttgaaagagcttcttcaacaaacatctcattacagctcaaaagtccgtaaagacgcattgttgggaattaaggatttattccttaagcatccagaagagttgaggttgcataagtatgctgttattgaaaaactgcgtgaacgcattggtgatgatgatagactagttcgagaaacgctgtatcaacttttcaagtcagtgattttccctggatttaaggaggataatcaagagctttttgtttccttgatgatggcctatatatttaattcaatgacacatttagcaattgatgttcgactgatggctttcaaatttttggagcttgttatccagtactatccaccttcctttttcttgtatgctgaaaagattcttcaaaactttgaggatattttgcgaaagaaccagttttatttagaggacaagagcaagctgaagaatgctctttctgggttggagcaatgcttgttactgcttccatttaacaagaaagaagttggctcatgtgaacagaatgatgctggacaagggatgctgcatgcttttgaccctgatgtacctgcagaatctgctgggttttctctcatcatcccaaaactaaaagaccttgtgccagttctagtcaattgtttccaagattttattcctgcagtacagactggtgcacatcttgatgtgcaatcatttgattgtatggtgtctatacttcacagtataaatcatgcagtccggttctttgtttatatgattgatgagggtacgtcagaatcacgaccttctcatggggagcttgatgtggctatgttgggtggaactatctccttgacgctgatgaagaagttacttgttctgtttcccctgaacatgagaaatcaactttctgagaaggatgatgttaaatattttgtcctggacagtgtgatgacagaaatatttctgcacttaagcaaatggatctgccctcccggtgttctgttggagaaatttttagaatttctagagaatgcgctgcttggaaagatttgtagtgatagacgctctggtaaagcaatccaggagaagcatttaatttcacttcttccttttgtaccgaaacttgtttcgcaagtggcaaatgactggaagtctcgtcttcttcaggcttttactaaggcattcaaggattgcaatcctgtgtcatctttaaaattagcttgtctttccacaatggaggagatggttgttcctagacaaggcatgctgtacctggatccaagggatcccgagatattaaactttcagattgcctggataagagagcttcccatgttgctaatcctgctgggtgataagaacacgtcccactcccaggttgtgttacaccttttacttcggctgggacagcgtgctttcatgaactattcctttgcattggaatatgacaacatgcaattctcactgcaagaatttttctgcatatatcaagatgatggaaatataatatatggtccttttgtaaggctgcccagagaatctcaggaactctctctttgttgccttcgttatatctctaatttggatttgcatatgttgaggtcaatagcttattgttgcctatgtccagaattggaacaatatgtaataattcgagtcatagagatcctgcattcagcttataaatctggacatattcaaattgcggatcacattagtttcttcatcactttgctctcccgcttcagagttttgccagaaaatgttaatgctgttatggagagagatgtgaatatctcaaatcgggggacttttaaatcaatcactggcattgtttgctcgtgtctgtcacagatgggcgatagttctcttgtttttaaaatactagaaaaaatggtactagatcaaatgttgcaaaaccttccgctagacaatgtctgtgctatgcttaggatgcttattgcactggactctgaagagacaataatttctgaacaagcttttgtaagcttagtcaaaatccttccgcgatatcttattgacattgtgcattgcattgctgaagatgatgggaaggcctctggttccctttcctcgagtgcatgctgttattatcttgttccttgctttttcttatttgttaaaagtcacagacttctgggtcttgtgtcgaagatgttggggtcatggataaatgaaagtttgtcaattttaccttgcgatcatacccactatgaaacagacatttcaagcagggtagaagcaatcgtttcagttcttctcttgttgcataaggatgacaaaatttggcaaattatgtcttcattcaaggcagagattgactgtattttgcaaagcatcatctctatacagtcctcagaggaaatcagcatgacccttcaagaaaagcacaaattgaaatgtgcacatgatagactaaagaacgccacaagtgcattgcatgctgagatgcagtcagtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ipi1_N

Protein Analysis

886

Amino Acids

100.8

Weight (kDa)

7.09

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AasI GACNNNNNNGTC 1 cut(s) 2337
Acc36I ACCTGC 1 cut(s) 772
AccII CGCG 2 cut(s) 42, 2206
AciI CCGC 5 cut(s) 42, 1883, 1918, 2102, 2204
AclWI GGATC 7 cut(s) 1166, 1448, 1457, 1461, 1470, 1837, 1893
AcsI RAATTY 5 cut(s) 515, 1187, 1196, 1649, 2481
AcuI CTGAAG 5 cut(s) 659, 1325, 1906, 2172, 2259
AfaI GTAC 7 cut(s) 539, 762, 858, 964, 1293, 1448, 2076
AfiI CCNNNNNNNGG 3 cut(s) 986, 1436, 1552
AflII CTTAAG 2 cut(s) 284, 1147
AflIII ACRYGT 1 cut(s) 1539
AhdI GACNNNNNGTC 1 cut(s) 1327
AhlI ACTAGT 1 cut(s) 364
AjiI CACGTC 1 cut(s) 1542
AjnI CCWGG 6 cut(s) 409, 1113, 1257, 1449, 1490, 1551
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
Alw21I GWGCWC 2 cut(s) 871, 2605
Alw26I GTCTC 2 cut(s) 1335, 2150
Alw44I GTGCAC 2 cut(s) 867, 2601
AlwI GGATC 7 cut(s) 1166, 1448, 1457, 1461, 1470, 1837, 1893
AlwNI CAGNNNCTG 1 cut(s) 773
Ama87I CYCGRG 2 cut(s) 1466, 2272
AoxI GGCC 2 cut(s) 456, 2253
ApaLI GTGCAC 2 cut(s) 867, 2601
ApeKI GCWGC 3 cut(s) 739, 1213, 1705
ApoI RAATTY 5 cut(s) 515, 1187, 1196, 1649, 2481
Asp700I GAANNNNTTC 2 cut(s) 573, 1137
AspLEI GCGC 2 cut(s) 42, 1213
AspS9I GGNCC 1 cut(s) 1691
AsuC2I CCSGG 1 cut(s) 1170
AsuHPI GGTGA 2 cut(s) 362, 1541
AvaI CYCGRG 2 cut(s) 1466, 2272
AvaII GGWCC 1 cut(s) 1691
BaeGI GKGCMC 2 cut(s) 871, 2605
BamHI GGATCC 2 cut(s) 1453, 1462
BauI CACGAG 1 cut(s) 2017
BbsI GAAGAC 2 cut(s) 1328, 2490
Bbv12I GWGCWC 2 cut(s) 871, 2605
BbvI GCAGC 3 cut(s) 726, 1200, 1692
BccI CCATC 6 cut(s) 448, 499, 1414, 1667, 2026, 2240
BciT130I CCWGG 6 cut(s) 411, 1115, 1259, 1451, 1492, 1553
BcnI CCSGG 1 cut(s) 1170
BcoDI GTCTC 2 cut(s) 1335, 2150
BcuI ACTAGT 1 cut(s) 364
BfaI CTAG 8 cut(s) 365, 824, 1202, 1431, 2063, 2078, 2105, 2659
BfmI CTRYAG 2 cut(s) 765, 852
BfrI CTTAAG 2 cut(s) 284, 1147
BfuAI ACCTGC 1 cut(s) 772
BisI GCNGC 3 cut(s) 740, 1214, 1706
BlsI GCNGC 3 cut(s) 741, 1215, 1707
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 7 cut(s) 411, 1115, 1170, 1259, 1451, 1492, 1553
Bme18I GGWCC 1 cut(s) 1691
BmeRI GACNNNNNGTC 1 cut(s) 1327
BmeT110I CYCGRG 2 cut(s) 1466, 2272
BmgBI CACGTC 1 cut(s) 1542
BmgT120I GGNCC 1 cut(s) 1691
BmiI GGNNCC 3 cut(s) 1455, 1464, 2263
BmrFI CCNGG 7 cut(s) 411, 1115, 1170, 1259, 1451, 1492, 1553
BmsI GCATC 7 cut(s) 167, 298, 712, 726, 2121, 2544, 2637
BpiI GAAGAC 2 cut(s) 1328, 2490
Bpu10I CCTNAGC 2 cut(s) 144, 2126
BpuMI CCSGG 1 cut(s) 1170
BsaBI GATNNNNATC 1 cut(s) 1371
BsaJI CCNNGG 3 cut(s) 409, 1457, 1551
BsaWI WCCGGW 1 cut(s) 933
Bsc4I CCNNNNNNNGG 3 cut(s) 986, 1436, 1552
Bse1I ACTGG 7 cut(s) 535, 610, 818, 868, 1328, 2008, 2148
Bse3DI GCAATG 4 cut(s) 671, 2227, 2232, 2633
Bse8I GATNNNNATC 1 cut(s) 1371
BseBI CCWGG 6 cut(s) 411, 1115, 1259, 1451, 1492, 1553
BseDI CCNNGG 3 cut(s) 409, 1457, 1551
BseGI GGATG 7 cut(s) 158, 289, 741, 792, 1099, 2136, 2479
BseJI GATNNNNATC 1 cut(s) 1371
BseLI CCNNNNNNNGG 3 cut(s) 986, 1436, 1552
BseMI GCAATG 4 cut(s) 671, 2227, 2232, 2633
BseMII CTCAG 6 cut(s) 105, 135, 1077, 1733, 2568, 2634
BseNI ACTGG 7 cut(s) 535, 610, 818, 868, 1328, 2008, 2148
BseRI GAGGAG 1 cut(s) 1430
BseSI GKGCMC 2 cut(s) 871, 2605
BseXI GCAGC 3 cut(s) 726, 1200, 1692
BseYI CCCAGC 3 cut(s) 776, 1525, 1579
BsgI GTGCAG 1 cut(s) 1127
Bsh1236I CGCG 2 cut(s) 42, 2206
BshFI GGCC 2 cut(s) 458, 2255
BsiHKAI GWGCWC 2 cut(s) 871, 2605
BsiHKCI CYCGRG 2 cut(s) 1466, 2272
BsiSI CCGG 2 cut(s) 934, 1170
BslFI GGGAC 3 cut(s) 1528, 1596, 1999
BslI CCNNNNNNNGG 3 cut(s) 986, 1436, 1552
BsmAI GTCTC 2 cut(s) 1335, 2150
BsmFI GGGAC 3 cut(s) 1528, 1596, 1999
BsmI GAATGC 5 cut(s) 106, 649, 1213, 1358, 1849
BsnI GGCC 2 cut(s) 458, 2255
BsoBI CYCGRG 2 cut(s) 1466, 2272
Bsp1286I GDGCHC 2 cut(s) 871, 2605
Bsp143I GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
BspACI CCGC 5 cut(s) 42, 1883, 1918, 2102, 2204
BspANI GGCC 2 cut(s) 458, 2255
BspCNI CTCAG 6 cut(s) 106, 136, 1078, 1732, 2567, 2635
BspFNI CGCG 2 cut(s) 42, 2206
BspHI TCATGA 1 cut(s) 1596
BspLI GGNNCC 3 cut(s) 1455, 1464, 2263
BspMAI CTGCAG 2 cut(s) 769, 856
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 7 cut(s) 1166, 1448, 1457, 1461, 1470, 1837, 1893
BspTI CTTAAG 2 cut(s) 284, 1147
BsrDI GCAATG 4 cut(s) 671, 2227, 2232, 2633
BsrI ACTGG 7 cut(s) 535, 610, 818, 868, 1328, 2008, 2148
BssECI CCNNGG 3 cut(s) 409, 1457, 1551
BssMI GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
BssSI CACGAG 1 cut(s) 2017
BssT1I CCWWGG 1 cut(s) 1457
Bst2BI CACGAG 1 cut(s) 2017
Bst2UI CCWGG 6 cut(s) 411, 1115, 1259, 1451, 1492, 1553
Bst4CI ACNGT 4 cut(s) 917, 1121, 2522, 2550
Bst6I CTCTTC 2 cut(s) 291, 2148
BstAFI CTTAAG 2 cut(s) 284, 1147
BstAPI GCANNNNNTGC 4 cut(s) 101, 773, 2013, 2639
BstC8I GCNNGC 6 cut(s) 635, 744, 1442, 1590, 2281, 2640
BstDEI CTNAG 9 cut(s) 114, 144, 1086, 1353, 1719, 2126, 2188, 2554, 2643
BstENI CCTNNNNNAGG 1 cut(s) 1434
BstF5I GGATG 7 cut(s) 158, 289, 741, 792, 1099, 2136, 2479
BstFNI CGCG 2 cut(s) 42, 2206
BstHHI GCGC 2 cut(s) 42, 1213
BstKTI GATC 7 cut(s) 1161, 1456, 1465, 1845, 1888, 2083, 2401
BstMAI GTCTC 2 cut(s) 1335, 2150
BstMBI GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
BstMWI GCNNNNNNNGC 9 cut(s) 101, 176, 773, 1522, 1585, 2013, 2131, 2630, 2639
BstNI CCWGG 6 cut(s) 411, 1115, 1259, 1451, 1492, 1553
BstNSI RCATGY 5 cut(s) 746, 1444, 1633, 2283, 2642
BstSCI CCNGG 7 cut(s) 409, 1113, 1168, 1257, 1449, 1490, 1551
BstSFI CTRYAG 2 cut(s) 765, 852
BstSLI GKGCMC 2 cut(s) 871, 2605
BstUI CGCG 2 cut(s) 42, 2206
BstV1I GCAGC 3 cut(s) 726, 1200, 1692
BstV2I GAAGAC 2 cut(s) 1328, 2490
BstX2I RGATCY 4 cut(s) 1158, 1453, 1462, 1842
BstYI RGATCY 4 cut(s) 1158, 1453, 1462, 1842
BsuRI GGCC 2 cut(s) 458, 2255
BtrI CACGTC 1 cut(s) 1542
BtsCI GGATG 7 cut(s) 158, 289, 741, 792, 1099, 2136, 2479
BtsI GCAGTG 2 cut(s) 186, 1640
BtsIMutI CAGTG 6 cut(s) 186, 405, 1126, 1640, 2001, 2141
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 6 cut(s) 635, 744, 1442, 1590, 2281, 2640
CaiI CAGNNNCTG 1 cut(s) 773
CciI TCATGA 1 cut(s) 1596
CfoI GCGC 2 cut(s) 42, 1213
Cfr13I GGNCC 1 cut(s) 1691
CseI GACGC 3 cut(s) 263, 1039, 1248
Csp6I GTAC 7 cut(s) 538, 761, 857, 963, 1292, 1447, 2075
CviQI GTAC 7 cut(s) 538, 761, 857, 963, 1292, 1447, 2075
DdeI CTNAG 9 cut(s) 114, 144, 1086, 1353, 1719, 2126, 2188, 2554, 2643
DpnI GATC 7 cut(s) 1160, 1455, 1464, 1844, 1887, 2082, 2400
DpnII GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
DraI TTTAAA 3 cut(s) 1389, 1993, 2056
DrdI GACNNNNNNGTC 1 cut(s) 2337
DriI GACNNNNNGTC 1 cut(s) 1327
DseDI GACNNNNNNGTC 1 cut(s) 2337
Eam1104I CTCTTC 2 cut(s) 291, 2148
Eam1105I GACNNNNNGTC 1 cut(s) 1327
EarI CTCTTC 2 cut(s) 291, 2148
Eco130I CCWWGG 1 cut(s) 1457
Eco147I AGGCCT 1 cut(s) 2255
Eco32I GATATC 1 cut(s) 2210
Eco47I GGWCC 1 cut(s) 1691
Eco57I CTGAAG 5 cut(s) 659, 1325, 1906, 2172, 2259
Eco88I CYCGRG 2 cut(s) 1466, 2272
EcoNI CCTNNNNNAGG 1 cut(s) 1434
EcoRII CCWGG 6 cut(s) 409, 1113, 1257, 1449, 1490, 1551
EcoRV GATATC 1 cut(s) 2210
EcoT14I CCWWGG 1 cut(s) 1457
ErhI CCWWGG 1 cut(s) 1457
FalI AAGNNNNNCTT 4 cut(s) 179, 211, 198, 230
FaqI GGGAC 3 cut(s) 1528, 1596, 1999
FauI CCCGC 1 cut(s) 1925
FauNDI CATATG 1 cut(s) 1770
Fnu4HI GCNGC 3 cut(s) 740, 1214, 1706
FokI GGATG 7 cut(s) 145, 276, 748, 779, 1106, 2143, 2486
Fsp4HI GCNGC 3 cut(s) 740, 1214, 1706
FspBI CTAG 8 cut(s) 365, 824, 1202, 1431, 2063, 2078, 2105, 2659
GlaI GCGC 2 cut(s) 41, 1212
GluI GCNGC 3 cut(s) 740, 1214, 1706
GsaI CCCAGC 3 cut(s) 780, 1529, 1583
HaeIII GGCC 2 cut(s) 458, 2255
HapII CCGG 2 cut(s) 934, 1170
HgaI GACGC 3 cut(s) 263, 1039, 1248
HhaI GCGC 2 cut(s) 42, 1213
Hin6I GCGC 2 cut(s) 40, 1211
HinP1I GCGC 2 cut(s) 40, 1211
HindIII AAGCTT 2 cut(s) 2175, 2185
HinfI GANTC 6 cut(s) 574, 770, 971, 1715, 1833, 2147
HpaII CCGG 2 cut(s) 934, 1170
HphI GGTGA 2 cut(s) 362, 1541
Hpy166II GTNNAC 5 cut(s) 184, 341, 713, 869, 2603
Hpy188I TCNGA 8 cut(s) 163, 970, 1087, 1485, 1925, 2152, 2170, 2557
Hpy8I GTNNAC 5 cut(s) 184, 341, 713, 869, 2603
HpyAV CCTTC 9 cut(s) 160, 558, 990, 1084, 1751, 2108, 2210, 2245, 2585
HpyCH4III ACNGT 4 cut(s) 917, 1121, 2522, 2550
HpyCH4IV ACGT 2 cut(s) 965, 1541
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 176, 773, 1522, 1585, 2013, 2131, 2630, 2639
HpyF3I CTNAG 9 cut(s) 114, 144, 1086, 1353, 1719, 2126, 2188, 2554, 2643
HpySE526I ACGT 2 cut(s) 965, 1541
HspAI GCGC 2 cut(s) 40, 1211
Kzo9I GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
LmnI GCTCC 3 cut(s) 523, 661, 991
Lsp1109I GCAGC 3 cut(s) 726, 1200, 1692
LweI GCATC 7 cut(s) 167, 298, 712, 726, 2121, 2544, 2637
MaeI CTAG 8 cut(s) 365, 824, 1202, 1431, 2063, 2078, 2105, 2659
MaeII ACGT 2 cut(s) 965, 1541
MaeIII GTNAC 5 cut(s) 672, 1044, 1560, 2025, 2324
MalI GATC 7 cut(s) 1160, 1455, 1464, 1844, 1887, 2082, 2400
MboI GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
MfeI CAATTG 3 cut(s) 132, 489, 829
MflI RGATCY 4 cut(s) 1158, 1453, 1462, 1842
MhlI GDGCHC 2 cut(s) 871, 2605
MlyI GAGTC 2 cut(s) 1842, 2141
MmeI TCCRAC 2 cut(s) 639, 1161
MroXI GAANNNNTTC 2 cut(s) 573, 1137
MslI CAYNNNNRTG 3 cut(s) 315, 876, 2472
MspCI CTTAAG 2 cut(s) 284, 1147
MspI CCGG 2 cut(s) 934, 1170
MspR9I CCNGG 7 cut(s) 411, 1115, 1170, 1259, 1451, 1492, 1553
MunI CAATTG 3 cut(s) 132, 489, 829
Mva1269I GAATGC 5 cut(s) 106, 649, 1213, 1358, 1849
MvaI CCWGG 6 cut(s) 411, 1115, 1259, 1451, 1492, 1553
MvnI CGCG 2 cut(s) 42, 2206
MwoI GCNNNNNNNGC 9 cut(s) 101, 176, 773, 1522, 1585, 2013, 2131, 2630, 2639
NciI CCSGG 1 cut(s) 1170
NdeI CATATG 1 cut(s) 1770
NdeII GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
NlaIV GGNNCC 3 cut(s) 1455, 1464, 2263
NmuCI GTSAC 2 cut(s) 2025, 2324
NspI RCATGY 5 cut(s) 746, 1444, 1633, 2283, 2642
PaeI GCATGC 4 cut(s) 746, 1444, 2283, 2642
PaeR7I CTCGAG 1 cut(s) 2272
PagI TCATGA 1 cut(s) 1596
PceI AGGCCT 1 cut(s) 2255
PctI GAATGC 5 cut(s) 106, 649, 1213, 1358, 1849
PdmI GAANNNNTTC 2 cut(s) 573, 1137
PfeI GAWTC 4 cut(s) 574, 770, 971, 1715
PflFI GACNNNGTC 1 cut(s) 2111
PfoI TCCNGGA 2 cut(s) 1113, 1257
PkrI GCNGC 3 cut(s) 741, 1215, 1707
PleI GAGTC 2 cut(s) 1841, 2141
PpsI GAGTC 2 cut(s) 1841, 2141
PsiI TTATAA 1 cut(s) 1860
Psp6I CCWGG 6 cut(s) 409, 1113, 1257, 1449, 1490, 1551
PspFI CCCAGC 3 cut(s) 776, 1525, 1579
PspGI CCWGG 6 cut(s) 409, 1113, 1257, 1449, 1490, 1551
PspN4I GGNNCC 3 cut(s) 1455, 1464, 2263
PspPI GGNCC 1 cut(s) 1691
PspXI VCTCGAGB 1 cut(s) 2272
PstI CTGCAG 2 cut(s) 769, 856
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 4 cut(s) 1158, 1453, 1462, 1842
PsyI GACNNNGTC 1 cut(s) 2111
RsaI GTAC 7 cut(s) 539, 762, 858, 964, 1293, 1448, 2076
RsaNI GTAC 7 cut(s) 538, 761, 857, 963, 1292, 1447, 2075
RseI CAYNNNNRTG 3 cut(s) 315, 876, 2472
SatI GCNGC 3 cut(s) 740, 1214, 1706
Sau3AI GATC 7 cut(s) 1158, 1453, 1462, 1842, 1885, 2080, 2398
Sau96I GGNCC 1 cut(s) 1691
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 2 cut(s) 1842, 2141
ScrFI CCNGG 7 cut(s) 411, 1115, 1170, 1259, 1451, 1492, 1553
SduI GDGCHC 2 cut(s) 871, 2605
SfaNI GCATC 7 cut(s) 167, 298, 712, 726, 2121, 2544, 2637
SfcI CTRYAG 2 cut(s) 765, 852
Sfr274I CTCGAG 1 cut(s) 2272
SinI GGWCC 1 cut(s) 1691
SlaI CTCGAG 1 cut(s) 2272
SmiMI CAYNNNNRTG 3 cut(s) 315, 876, 2472
SmlI CTYRAG 3 cut(s) 284, 1147, 2272
SmoI CTYRAG 3 cut(s) 284, 1147, 2272
SpeI ACTAGT 1 cut(s) 364
SphI GCATGC 4 cut(s) 746, 1444, 2283, 2642
SseBI AGGCCT 1 cut(s) 2255
SsiI CCGC 5 cut(s) 42, 1883, 1918, 2102, 2204
SspI AATATT 2 cut(s) 1106, 1137
SspMI CTAG 8 cut(s) 365, 824, 1202, 1431, 2063, 2078, 2105, 2659
StuI AGGCCT 1 cut(s) 2255
StyD4I CCNGG 7 cut(s) 409, 1113, 1168, 1257, 1449, 1490, 1551
StyI CCWWGG 1 cut(s) 1457
TaaI ACNGT 4 cut(s) 917, 1121, 2522, 2550
TaiI ACGT 2 cut(s) 968, 1544
TaqI TCGA 6 cut(s) 48, 370, 499, 1831, 2273, 2348
TatI WGTACW 2 cut(s) 537, 856
TfiI GAWTC 4 cut(s) 574, 770, 971, 1715
TscAI CASTG 6 cut(s) 186, 405, 1126, 1647, 2008, 2148
TseFI GTSAC 2 cut(s) 2025, 2324
TseI GCWGC 3 cut(s) 739, 1213, 1705
Tsp45I GTSAC 2 cut(s) 2025, 2324
TspDTI ATGAA 7 cut(s) 1052, 1585, 1613, 1891, 2388, 2427, 2490
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 6 cut(s) 186, 405, 1126, 1647, 2008, 2148
Tth111I GACNNNGTC 1 cut(s) 2111
Vha464I CTTAAG 2 cut(s) 284, 1147
VneI GTGCAC 2 cut(s) 867, 2601
VpaK11BI GGWCC 1 cut(s) 1691
XagI CCTNNNNNAGG 1 cut(s) 1434
XapI RAATTY 5 cut(s) 515, 1187, 1196, 1649, 2481
XbaI TCTAGA 1 cut(s) 1201
XceI RCATGY 5 cut(s) 746, 1444, 1633, 2283, 2642
XhoI CTCGAG 1 cut(s) 2272
XmnI GAANNNNTTC 2 cut(s) 573, 1137
XspI CTAG 8 cut(s) 365, 824, 1202, 1431, 2063, 2078, 2105, 2659
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.