pycom14g03330

Testis-expressed sequence 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001389
Physical Location & Seq
Reverse (-)
2655003 .. 2657325
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03330.3

Sequence Viewer

Length: 735 bp
ATGGCAGAAAATATCCATCCTGTTAGAGAGAGTGATGTAAATATCTCAAATCGGGGGACTTTTAAGTCGCTCAGCCGAATTGTCTGCTCATGTCTGTCACAGATGGGTGATAATTCTCTTGTTTTTCAGATGCTAGAGAAAATGATACTTGATCAGATGTTGCAAAATCCTTCTTTGGACAACGTTTGTGCTATGCTAAGGACACTTATTACACTGGATTCTAAAGCCATGATAATTTCTCAAGGAGCTTTTATAAGCCTAGGCAATATCCTTCTGCGGTATCTGATTGACGTTGTGCAGTTCATTGTTGAAGATGACAAGAACACCACTGTTTCCATTTCTTCAAGTGCATGCCGTTATTATCTCATTCCTTGCTTTGTCTTGTTTGATAATGGTCACGAACTTCTGAATCTTGTATTGAATACATTGGGGTCATGGATTACACAAAGTAGTTTGCCGCATCTAACTGGTGATCACACCCGCTACGCAACTGACATTTCAAGTATGGTTGATGCGGTCGTTTACCTGCTTCAGCTGCTGCATAAGGATGACAAAATCTGTCAAATCATGTCTTCGTTCATGGCGGCGATTGACTATATCATGGCAGGCATCAACTTATTACAGTCATCAGAGGAAATCAGCGTGACGATAGCAGAAGGGCACAAGGTGCAATGTGCACTTGGTAGACTGCAGAATGTAATAACTACTCTGCAGGGTGAGATTCAGTCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ipi1_N

Protein Analysis

245

Amino Acids

27.08

Weight (kDa)

4.99

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AasI GACNNNNNNGTC 1 cut(s) 64
Acc36I ACCTGC 1 cut(s) 534
AccI GTMKAC 1 cut(s) 685
AciI CCGC 5 cut(s) 277, 458, 481, 515, 584
AclI AACGTT 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 515
AdeI CACNNNGTG 1 cut(s) 667
AgsI TTSAA 4 cut(s) 311, 345, 421, 501
AluBI AGCT 2 cut(s) 248, 535
AluI AGCT 2 cut(s) 248, 535
Alw21I GWGCWC 1 cut(s) 679
Alw44I GTGCAC 1 cut(s) 675
AlwNI CAGNNNCTG 1 cut(s) 538
ApaLI GTGCAC 1 cut(s) 675
ApeKI GCWGC 2 cut(s) 535, 538
AspA2I CCTAGG 1 cut(s) 259
AsuHPI GGTGA 3 cut(s) 119, 482, 728
AvrII CCTAGG 1 cut(s) 259
BaeGI GKGCMC 2 cut(s) 663, 679
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 679
BbvI GCAGC 2 cut(s) 522, 525
BccI CCATC 2 cut(s) 24, 97
BceAI ACGGC 1 cut(s) 339
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BclI TGATCA 2 cut(s) 151, 472
BfaI CTAG 3 cut(s) 134, 260, 733
BfmI CTRYAG 2 cut(s) 689, 710
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 4 cut(s) 458, 536, 539, 585
BlnI CCTAGG 1 cut(s) 259
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 4 cut(s) 459, 537, 540, 586
BmsI GCATC 4 cut(s) 120, 469, 502, 618
BpiI GAAGAC 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 197
Bpu1102I GCTNAGC 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 259
Bse1I ACTGG 2 cut(s) 219, 472
Bse3DI GCAATG 1 cut(s) 677
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 16, 553
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 219, 472
BseSI GKGCMC 2 cut(s) 663, 679
BseXI GCAGC 2 cut(s) 522, 525
BsgI GTGCAG 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 519
BsiEI CGRYCG 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 679
BslFI GGGAC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 663, 679
Bsp143I GATC 2 cut(s) 151, 472
Bsp1720I GCTNAGC 1 cut(s) 71
BspACI CCGC 5 cut(s) 277, 458, 481, 515, 584
BspCNI CTCAG 1 cut(s) 84
BspMAI CTGCAG 2 cut(s) 693, 714
BspMI ACCTGC 1 cut(s) 534
BsrDI GCAATG 1 cut(s) 677
BsrI ACTGG 2 cut(s) 219, 472
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 2 cut(s) 151, 472
BssT1I CCWWGG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 331, 624
BstAPI GCANNNNNTGC 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 352, 607
BstDEI CTNAG 2 cut(s) 71, 197
BstF5I GGATG 2 cut(s) 16, 553
BstKTI GATC 2 cut(s) 154, 475
BstMBI GATC 2 cut(s) 151, 472
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 2 cut(s) 535, 667
BstNSI RCATGY 1 cut(s) 354
BstSFI CTRYAG 2 cut(s) 689, 710
BstSLI GKGCMC 2 cut(s) 663, 679
BstV1I GCAGC 2 cut(s) 522, 525
BstV2I GAAGAC 1 cut(s) 564
BtsCI GGATG 2 cut(s) 16, 553
BtsIMutI CAGTG 2 cut(s) 212, 327
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 2 cut(s) 352, 607
CaiI CAGNNNCTG 1 cut(s) 538
CviAII CATG 7 cut(s) 90, 229, 351, 435, 568, 580, 601
CviJI RGCY 5 cut(s) 75, 227, 248, 258, 535
CviKI_1 RGCY 5 cut(s) 75, 227, 248, 258, 535
DdeI CTNAG 2 cut(s) 71, 197
DpnI GATC 2 cut(s) 153, 474
DpnII GATC 2 cut(s) 151, 472
DraIII CACNNNGTG 1 cut(s) 667
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 7 cut(s) 93, 232, 354, 438, 571, 583, 604
FaqI GGGAC 1 cut(s) 70
FatI CATG 7 cut(s) 89, 228, 350, 434, 567, 579, 600
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 2 cut(s) 151, 472
FblI GTMKAC 1 cut(s) 685
Fnu4HI GCNGC 4 cut(s) 458, 536, 539, 585
FokI GGATG 2 cut(s) 3, 560
Fsp4HI GCNGC 4 cut(s) 458, 536, 539, 585
FspBI CTAG 3 cut(s) 134, 260, 733
GluI GCNGC 4 cut(s) 458, 536, 539, 585
Hin1II CATG 7 cut(s) 93, 232, 354, 438, 571, 583, 604
HinfI GANTC 3 cut(s) 218, 409, 721
HphI GGTGA 3 cut(s) 119, 482, 728
Hpy166II GTNNAC 3 cut(s) 523, 677, 686
Hpy188I TCNGA 5 cut(s) 129, 156, 285, 408, 631
Hpy188III TCNNGA 1 cut(s) 398
Hpy8I GTNNAC 3 cut(s) 523, 677, 686
HpyAV CCTTC 3 cut(s) 180, 281, 650
HpyCH4III ACNGT 2 cut(s) 331, 624
HpyCH4IV ACGT 2 cut(s) 183, 291
HpyCH4V TGCA 8 cut(s) 163, 298, 350, 541, 670, 677, 691, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 535, 667
HpyF3I CTNAG 2 cut(s) 71, 197
HpySE526I ACGT 2 cut(s) 183, 291
Hsp92II CATG 7 cut(s) 93, 232, 354, 438, 571, 583, 604
Ksp22I TGATCA 2 cut(s) 151, 472
Kzo9I GATC 2 cut(s) 151, 472
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 6 cut(s) 33, 200, 453, 539, 591, 698
Lsp1109I GCAGC 2 cut(s) 522, 525
LweI GCATC 4 cut(s) 120, 469, 502, 618
MaeI CTAG 3 cut(s) 134, 260, 733
MaeII ACGT 2 cut(s) 183, 291
MaeIII GTNAC 3 cut(s) 96, 395, 643
MalI GATC 2 cut(s) 153, 474
MboI GATC 2 cut(s) 151, 472
MboII GAAGA 3 cut(s) 323, 333, 564
MhlI GDGCHC 2 cut(s) 663, 679
MluCI AATT 3 cut(s) 78, 112, 234
MnlI CCTC 1 cut(s) 625
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 535
MwoI GCNNNNNNNGC 2 cut(s) 535, 667
NdeII GATC 2 cut(s) 151, 472
NlaIII CATG 7 cut(s) 93, 232, 354, 438, 571, 583, 604
NmuCI GTSAC 3 cut(s) 96, 395, 643
NspI RCATGY 1 cut(s) 354
PaeI GCATGC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 218, 409, 721
PkrI GCNGC 4 cut(s) 459, 537, 540, 586
PsiI TTATAA 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 183
PstI CTGCAG 2 cut(s) 693, 714
PstNI CAGNNNCTG 1 cut(s) 538
PvuII CAGCTG 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 4 cut(s) 458, 536, 539, 585
Sau3AI GATC 2 cut(s) 151, 472
SduI GDGCHC 2 cut(s) 663, 679
SetI ASST 6 cut(s) 186, 250, 294, 528, 537, 669
SfaNI GCATC 4 cut(s) 120, 469, 502, 618
SfcI CTRYAG 2 cut(s) 689, 710
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SphI GCATGC 1 cut(s) 354
Sse9I AATT 3 cut(s) 78, 112, 234
SsiI CCGC 5 cut(s) 277, 458, 481, 515, 584
SspMI CTAG 3 cut(s) 134, 260, 733
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 331, 624
TaiI ACGT 2 cut(s) 186, 294
TasI AATT 3 cut(s) 78, 112, 234
TauI GCSGC 2 cut(s) 460, 587
TfiI GAWTC 3 cut(s) 218, 409, 721
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 219, 334
TseFI GTSAC 3 cut(s) 96, 395, 643
TseI GCWGC 2 cut(s) 535, 538
Tsp45I GTSAC 3 cut(s) 96, 395, 643
TspDTI ATGAA 2 cut(s) 292, 568
TspRI CASTG 2 cut(s) 219, 334
VneI GTGCAC 1 cut(s) 675
XceI RCATGY 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 685
XspI CTAG 3 cut(s) 134, 260, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.