RchiOBHm_Chr1g0331091

Testis-expressed sequence 10 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001389
Physical Location & Seq
Reverse (-)
20911484 .. 20914136
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56015

Sequence Viewer

Length: 591 bp
ATGATCAGCAATTGTTCTTCCCGAGCAGAGTGTGGCATCCGAGAAGGCAGGATTAGCAGTGAACAAGAAAGGCTTGACTTTGAAAGAGCTTCTTCAACAAATATCTCATTACAGCTCAAAAACGCATTGTTGGGAATTAAGGATTTATTCTTTAAGCATCCAGAAGAGTTGAGGTTGCATAAGTACGCTGTTATTGAAAAACTGTGTGAACGCATTGGTGATGATGATAGACTAGTTCGAGAAACGCTGTATCAACTTTTCAAATCAGTGATTTTCCCTGGATTTAAGGAGGATAATCAAGAGCTTTTTGTTTCCTTGATGATGGCCTATATATTTAATTCAATGACACATTTAGCAATTGATGTTCGACTGATGGCTTTCAAATTTTTGGAGCTTGTTATCCAGTACTATCCACCTTCCTTTTTCTTGTATGCTGAAAAGTTTCCGGCAGCCAGCGAACCTCTCCAGATCTCCAGTTTCCGGCACTGCTCTAGTCTGTTTGGCCCGAACGCTTGCCGCTCCGTCCCCACCAGTTTGCGGCGTCATCGGCATCTGTCTCGACCAGCCTCCATGCGCCAGTTCCTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ipi1_N

Protein Analysis

196

Amino Acids

22.81

Weight (kDa)

8.84

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ipi1_N PF12333 101 - 140 2e-06 Rix1 complex component involved in 60S ribosome maturation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 519
AciI CCGC 2 cut(s) 517, 538
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 541
AfaI GTAC 2 cut(s) 185, 407
AfiI CCNNNNNNNGG 2 cut(s) 480, 537
AgsI TTSAA 6 cut(s) 83, 96, 197, 262, 342, 382
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 4 cut(s) 89, 115, 304, 394
AluI AGCT 4 cut(s) 89, 115, 304, 394
Alw26I GTCTC 1 cut(s) 561
Ama87I CYCGRG 1 cut(s) 21
AoxI GGCC 2 cut(s) 324, 502
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 21
BbvI GCAGC 1 cut(s) 461
BccI CCATC 2 cut(s) 316, 367
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 279
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 561
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 2 cut(s) 233, 492
BglII AGATCT 1 cut(s) 468
BisI GCNGC 3 cut(s) 450, 517, 539
BlsI GCNGC 3 cut(s) 451, 518, 540
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 279
BmeT110I CYCGRG 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 3 cut(s) 45, 166, 559
BpmI CTGGAG 2 cut(s) 449, 457
BsaHI GRCGYC 1 cut(s) 541
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 2 cut(s) 480, 537
Bse1I ACTGG 4 cut(s) 403, 474, 531, 577
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 36, 157
BseLI CCNNNNNNNGG 2 cut(s) 480, 537
BseNI ACTGG 4 cut(s) 403, 474, 531, 577
BseXI GCAGC 1 cut(s) 461
BshFI GGCC 2 cut(s) 326, 504
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 2 cut(s) 446, 481
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 480, 537
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 2 cut(s) 326, 504
BsoBI CYCGRG 1 cut(s) 21
Bsp143I GATC 2 cut(s) 3, 468
BspACI CCGC 2 cut(s) 517, 538
BspANI GGCC 2 cut(s) 326, 504
BsrBI CCGCTC 1 cut(s) 519
BsrI ACTGG 4 cut(s) 403, 474, 531, 577
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 2 cut(s) 3, 468
BssNI GRCGYC 1 cut(s) 541
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 204
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 541
BstC8I GCNNGC 2 cut(s) 454, 514
BstF5I GGATG 2 cut(s) 36, 157
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 2 cut(s) 6, 471
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 2 cut(s) 3, 468
BstMWI GCNNNNNNNGC 2 cut(s) 54, 547
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 2 cut(s) 326, 504
BtsCI GGATG 2 cut(s) 36, 157
BtsI GCAGTG 2 cut(s) 64, 484
BtsIMutI CAGTG 3 cut(s) 64, 273, 484
Cac8I GCNNGC 2 cut(s) 454, 514
CfoI GCGC 1 cut(s) 576
Cfr13I GGNCC 1 cut(s) 503
CseI GACGC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 184, 406
CviAII CATG 1 cut(s) 571
CviQI GTAC 2 cut(s) 184, 406
DpnI GATC 2 cut(s) 5, 470
DpnII GATC 2 cut(s) 3, 468
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 277
FaeI CATG 1 cut(s) 574
FaiI YATR 5 cut(s) 180, 330, 332, 432, 572
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 76, 108
FaqI GGGAC 1 cut(s) 509
FatI CATG 1 cut(s) 570
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 450, 517, 539
FokI GGATG 2 cut(s) 23, 144
Fsp4HI GCNGC 3 cut(s) 450, 517, 539
FspBI CTAG 2 cut(s) 233, 492
GlaI GCGC 1 cut(s) 575
GluI GCNGC 3 cut(s) 450, 517, 539
GsuI CTGGAG 2 cut(s) 449, 457
HaeIII GGCC 2 cut(s) 326, 504
HapII CCGG 2 cut(s) 446, 481
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 576
Hin1I GRCGYC 1 cut(s) 541
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HpaII CCGG 2 cut(s) 446, 481
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 62, 209
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 6 cut(s) 21, 161, 239, 299, 466, 558
Hpy8I GTNNAC 2 cut(s) 62, 209
HpyAV CCTTC 2 cut(s) 38, 426
HpyCH4III ACNGT 1 cut(s) 204
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 547
Hsp92I GRCGYC 1 cut(s) 541
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 468
LmnI GCTCC 2 cut(s) 391, 524
Lsp1109I GCAGC 1 cut(s) 461
LweI GCATC 3 cut(s) 45, 166, 559
MaeI CTAG 2 cut(s) 233, 492
MalI GATC 2 cut(s) 5, 470
MbiI CCGCTC 1 cut(s) 519
MboI GATC 2 cut(s) 3, 468
MboII GAAGA 3 cut(s) 9, 84, 176
MfeI CAATTG 2 cut(s) 10, 357
MflI RGATCY 1 cut(s) 468
MluCI AATT 5 cut(s) 10, 135, 337, 357, 383
MnlI CCTC 4 cut(s) 165, 283, 471, 577
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 4 cut(s) 138, 153, 285, 336
MspI CCGG 2 cut(s) 446, 481
MspR9I CCNGG 1 cut(s) 279
MunI CAATTG 2 cut(s) 10, 357
MvaI CCWGG 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 54, 547
NdeII GATC 2 cut(s) 3, 468
NlaIII CATG 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 441
PkrI GCNGC 3 cut(s) 451, 518, 540
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 1 cut(s) 503
PsuI RGATCY 1 cut(s) 468
RsaI GTAC 2 cut(s) 185, 407
RsaNI GTAC 2 cut(s) 184, 406
SaqAI TTAA 4 cut(s) 138, 153, 285, 336
SatI GCNGC 3 cut(s) 450, 517, 539
Sau3AI GATC 2 cut(s) 3, 468
Sau96I GGNCC 1 cut(s) 503
ScaI AGTACT 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 279
SetI ASST 7 cut(s) 91, 117, 176, 306, 396, 418, 463
SfaNI GCATC 3 cut(s) 45, 166, 559
SpeI ACTAGT 1 cut(s) 232
Sse9I AATT 5 cut(s) 10, 135, 337, 357, 383
SsiI CCGC 2 cut(s) 517, 538
SspMI CTAG 2 cut(s) 233, 492
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 238, 367, 559
TasI AATT 5 cut(s) 10, 135, 337, 357, 383
TatI WGTACW 1 cut(s) 405
TauI GCSGC 2 cut(s) 519, 541
Tru1I TTAA 4 cut(s) 138, 153, 285, 336
Tru9I TTAA 4 cut(s) 138, 153, 285, 336
TscAI CASTG 3 cut(s) 64, 273, 491
TseI GCWGC 1 cut(s) 449
TspGWI ACGGA 1 cut(s) 511
TspRI CASTG 3 cut(s) 64, 273, 491
XapI RAATTY 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 233, 492
ZrmI AGTACT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.