MD14G1038600.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004370
Physical Location & Seq
Forward (+)
3515673 .. 3519478
3806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1038600.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGCAGCAGCAGCAACAACAGCCGCAAGCAACGACGCAGAACTCCACGCCGCCGCAGCCTTCCTTTGCGGGGTCCACCACGGCGGAAGCACCGCCAAAACAGGTGGCGCAGGCGATGGAGCGGCTTTCTCAAGCCAGCCGCCTCATCGCGGACATTAGGATTGGCGCCGACCGCCTCCTCGAAGCCCTGTTCGTGGCCGCTCAGCCGCACCAGAGCTCCAAGCCTCTCCAGTTGTTCCTCAACGAGGACGCTTCCATGCGCCAACACCTCCTCGACCTCCGCTCCGTCGGAAGACAGCTGGAAGAATCTGGAGTTTTGAACGAGTCTCTGCGATCGCGGAGCAATTCTTGGGGATTACACATGCCGTTGGTTTGTCCGGATGGCGCCGTGGTTGCGTATGCTTGGAAGCGGCAGCTCGCCGGCCAAGCTGGTGCTTCTGCAGTTGACAGGACCAGGCTAGCTCTCAAGGCATTCACTGATCAGAAAAGGCGATTTTTTCCTCACCTTGATGATGCATCAAATGATCAGTGTAATGAATCAGCTTCGAAGAAGCAACGCATTTCTCAAGCACCCGCAGCACATAATCAAGAAGATGGTATAGATTGCAAAACGCTATCAGAGGTTTTAGTGCGTATGGAAAAGGTTCCTCATTTGAAAGTTTCCATATATGAACGATTGGATTGGTTGAAACGAGCATCTTCACTGCCAGAAAATGAGAGTTCCAGTGAAACATTAAAGGATCATAGTTATCATAGTTCAAGTAAGTTACGATCAGGTCCGCCTGATGTTGCACCAGATAAGGTTGCTGTCATTGAGTTGCTGTTTCCTTCTACCTTCAGAGCTGTAGTTTCATTGCATCCTGCTGGTTCTATTGACCCAGATGCAGTGGCATTTTTTTCTCCAGATGAGGGAGGCAGCTACATACATGCAAGAGGTGTTTCAATTTATCATGTCTATAAGCATATCACGGAACATGCTGCTACTGCTTTGCAGTATTTCATTGGAGTTCGAGCTGAAACAGCTCTACATTGTCTTTTGCATTGGATCTGCAGCTATCAAACTCTCTTTACAAAAGTTTGCAGCAAGTGTGGACGGCTGCTAGCAATGGACCGGAAATCAGCTCAACTGTTACCGCCAGTTTATCGTCCTTATCGGCAGTTCACTACATTGAATGTGTCATCAAGTTTGAATGTTTCCGGGGCTTACCATGTAGGTTGTTTCTCAGAGGAATTGTGA

Protein Analysis

412

Amino Acids

45.5

Weight (kDa)

8.35

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med27 PF11571 313 - 406 2.2e-19 Mediator complex subunit 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 383
AccBSI CCGCTC 3 cut(s) 121, 200, 282
AccII CGCG 2 cut(s) 149, 337
AccIII TCCGGA 1 cut(s) 376
AclWI GGATC 2 cut(s) 747, 1052
AcoI YGGCCR 2 cut(s) 195, 421
AcuI CTGAAG 1 cut(s) 820
AcyI GRCGYC 2 cut(s) 165, 384
AfiI CCNNNNNNNGG 5 cut(s) 69, 148, 193, 244, 908
AgsI TTSAA 7 cut(s) 319, 655, 688, 759, 942, 1171, 1189
AjnI CCWGG 1 cut(s) 452
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 2 cut(s) 747, 1052
Aor13HI TCCGGA 1 cut(s) 376
AoxI GGCC 2 cut(s) 195, 421
AsiSI GCGATCGC 1 cut(s) 335
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 4 cut(s) 109, 167, 261, 386
AspS9I GGNCC 4 cut(s) 72, 450, 776, 1108
AsuC2I CCSGG 1 cut(s) 1198
AsuHPI GGTGA 1 cut(s) 494
AsuII TTCGAA 1 cut(s) 545
AsuNHI GCTAGC 2 cut(s) 457, 1099
AvaII GGWCC 4 cut(s) 72, 450, 776, 1108
BanI GGYRCC 2 cut(s) 164, 383
BanII GRGCYC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 218
BccI CCATC 3 cut(s) 109, 374, 587
BceAI ACGGC 4 cut(s) 96, 349, 371, 1109
BciT130I CCWGG 1 cut(s) 454
BclI TGATCA 2 cut(s) 478, 523
BcnI CCSGG 1 cut(s) 1198
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 2 cut(s) 458, 1100
BfmI CTRYAG 3 cut(s) 438, 843, 1048
BfoI RGCGCY 2 cut(s) 168, 387
BlpI GCTNAGC 1 cut(s) 201
Bme1390I CCNGG 2 cut(s) 454, 1198
Bme18I GGWCC 4 cut(s) 72, 450, 776, 1108
BmgT120I GGNCC 4 cut(s) 72, 450, 776, 1108
BmiI GGNNCC 4 cut(s) 73, 166, 385, 645
BmrFI CCNGG 2 cut(s) 454, 1198
BmsI GCATC 5 cut(s) 502, 524, 704, 865, 871
BmtI GCTAGC 2 cut(s) 461, 1103
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 3 cut(s) 212, 330, 885
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 545
BpuEI CTTGAG 3 cut(s) 114, 449, 549
BpuMI CCSGG 1 cut(s) 1198
BsaHI GRCGYC 2 cut(s) 165, 384
BsaJI CCNNGG 3 cut(s) 78, 387, 1197
BsaWI WCCGGW 2 cut(s) 376, 1110
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 266, 296
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 148, 193, 244, 908
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 3 cut(s) 229, 723, 1136
Bse3DI GCAATG 2 cut(s) 851, 1110
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 78, 387, 1197
BseGI GGATG 2 cut(s) 385, 856
BseLI CCNNNNNNNGG 5 cut(s) 69, 148, 193, 244, 908
BseMI GCAATG 2 cut(s) 851, 1110
BseMII CTCAG 2 cut(s) 215, 1236
BseNI ACTGG 3 cut(s) 229, 723, 1136
BseRI GAGGAG 2 cut(s) 167, 260
Bsh1236I CGCG 2 cut(s) 149, 337
Bsh1285I CGRYCG 2 cut(s) 172, 335
BshFI GGCC 2 cut(s) 197, 423
BshNI GGYRCC 2 cut(s) 164, 383
BsiEI CGRYCG 2 cut(s) 172, 335
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 4 cut(s) 377, 420, 1111, 1197
BslI CCNNNNNNNGG 5 cut(s) 69, 148, 193, 244, 908
BsmAI GTCTC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 2 cut(s) 197, 423
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 218
Bsp13I TCCGGA 1 cut(s) 376
Bsp143I GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
Bsp1720I GCTNAGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 197, 423
BspCNI CTCAG 2 cut(s) 214, 1235
BspEI TCCGGA 1 cut(s) 376
BspFNI CGCG 2 cut(s) 149, 337
BspLI GGNNCC 4 cut(s) 73, 166, 385, 645
BspMAI CTGCAG 2 cut(s) 442, 1052
BspOI GCTAGC 2 cut(s) 461, 1103
BspPI GGATC 2 cut(s) 747, 1052
BspT104I TTCGAA 1 cut(s) 545
BspT107I GGYRCC 2 cut(s) 164, 383
BsrBI CCGCTC 3 cut(s) 121, 200, 282
BsrDI GCAATG 2 cut(s) 851, 1110
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 3 cut(s) 229, 723, 1136
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 78, 387, 1197
BssMI GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
BssNI GRCGYC 2 cut(s) 165, 384
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 1 cut(s) 1128
BstACI GRCGYC 2 cut(s) 165, 384
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 7 cut(s) 27, 111, 136, 417, 421, 459, 1101
BstDEI CTNAG 2 cut(s) 201, 1222
BstDSI CCRYGG 2 cut(s) 78, 387
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 2 cut(s) 385, 856
BstFNI CGCG 2 cut(s) 149, 337
BstH2I RGCGCY 2 cut(s) 168, 387
BstHHI GCGC 4 cut(s) 109, 167, 261, 386
BstKTI GATC 6 cut(s) 335, 481, 526, 742, 773, 1047
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
BstMCI CGRYCG 2 cut(s) 172, 335
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 3 cut(s) 364, 929, 977
BstSCI CCNGG 2 cut(s) 452, 1196
BstSFI CTRYAG 3 cut(s) 438, 843, 1048
BstUI CGCG 2 cut(s) 149, 337
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 1044
BstYI RGATCY 1 cut(s) 1044
BsuRI GGCC 2 cut(s) 197, 423
BtgI CCRYGG 2 cut(s) 78, 387
BtgZI GCGATG 2 cut(s) 128, 130
BtsCI GGATG 2 cut(s) 385, 856
BtsI GCAGTG 2 cut(s) 701, 891
BtsIMutI CAGTG 5 cut(s) 474, 533, 701, 730, 891
Cac8I GCNNGC 7 cut(s) 27, 111, 136, 417, 421, 459, 1101
CfoI GCGC 4 cut(s) 109, 167, 261, 386
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 4 cut(s) 72, 450, 776, 1108
CseI GACGC 2 cut(s) 43, 257
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 6 cut(s) 256, 361, 926, 950, 974, 1208
DdeI CTNAG 2 cut(s) 201, 1222
DinI GGCGCC 2 cut(s) 166, 385
DpnI GATC 6 cut(s) 334, 480, 525, 741, 772, 1046
DpnII GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
EaeI YGGCCR 2 cut(s) 195, 421
EciI GGCGGA 2 cut(s) 98, 768
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 218
Eco47I GGWCC 4 cut(s) 72, 450, 776, 1108
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 820
EcoICRI GAGCTC 1 cut(s) 216
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 218
EgeI GGCGCC 2 cut(s) 166, 385
EheI GGCGCC 2 cut(s) 166, 385
FaeI CATG 6 cut(s) 259, 364, 929, 953, 977, 1211
FatI CATG 6 cut(s) 255, 360, 925, 949, 973, 1207
FauI CCCGC 2 cut(s) 61, 580
FbaI TGATCA 2 cut(s) 478, 523
FokI GGATG 2 cut(s) 392, 843
FriOI GRGCYC 1 cut(s) 218
FspBI CTAG 2 cut(s) 458, 1100
GlaI GCGC 4 cut(s) 108, 166, 260, 385
GsuI CTGGAG 3 cut(s) 212, 330, 885
HaeII RGCGCY 2 cut(s) 168, 387
HaeIII GGCC 2 cut(s) 197, 423
HapII CCGG 4 cut(s) 377, 420, 1111, 1197
HgaI GACGC 2 cut(s) 43, 257
HhaI GCGC 4 cut(s) 109, 167, 261, 386
Hin1I GRCGYC 2 cut(s) 165, 384
Hin1II CATG 6 cut(s) 259, 364, 929, 953, 977, 1211
Hin6I GCGC 4 cut(s) 107, 165, 259, 384
HinP1I GCGC 4 cut(s) 107, 165, 259, 384
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 3 cut(s) 305, 323, 536
HpaII CCGG 4 cut(s) 377, 420, 1111, 1197
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 4 cut(s) 75, 445, 1091, 1161
Hpy188I TCNGA 5 cut(s) 290, 483, 619, 839, 1225
Hpy188III TCNNGA 4 cut(s) 309, 377, 587, 902
Hpy8I GTNNAC 4 cut(s) 75, 445, 1091, 1161
Hpy99I CGWCG 2 cut(s) 37, 290
HpyAV CCTTC 3 cut(s) 69, 837, 844
HpyCH4III ACNGT 1 cut(s) 1128
HpyF3I CTNAG 2 cut(s) 201, 1222
Hsp92I GRCGYC 2 cut(s) 165, 384
Hsp92II CATG 6 cut(s) 259, 364, 929, 953, 977, 1211
HspAI GCGC 4 cut(s) 107, 165, 259, 384
KasI GGCGCC 2 cut(s) 164, 383
Kpn2I TCCGGA 1 cut(s) 376
KroI GCCGGC 1 cut(s) 419
KroNI GCCGGC 1 cut(s) 421
Ksp22I TGATCA 2 cut(s) 478, 523
Kzo9I GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
LmnI GCTCC 4 cut(s) 118, 221, 287, 339
LweI GCATC 5 cut(s) 502, 524, 704, 865, 871
MaeI CTAG 2 cut(s) 458, 1100
MaeIII GTNAC 2 cut(s) 765, 1128
MalI GATC 6 cut(s) 334, 480, 525, 741, 772, 1046
MbiI CCGCTC 3 cut(s) 121, 200, 282
MboI GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
MboII GAAGA 5 cut(s) 303, 314, 559, 602, 690
MflI RGATCY 1 cut(s) 1044
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 3 cut(s) 343, 942, 1229
Mly113I GGCGCC 2 cut(s) 165, 384
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 268
Mph1103I ATGCAT 1 cut(s) 517
MroI TCCGGA 1 cut(s) 376
MroNI GCCGGC 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 1 cut(s) 734
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 4 cut(s) 377, 420, 1111, 1197
MspR9I CCNGG 2 cut(s) 454, 1198
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 2 cut(s) 149, 337
NaeI GCCGGC 1 cut(s) 421
NarI GGCGCC 2 cut(s) 165, 384
NciI CCSGG 1 cut(s) 1198
NdeII GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
NgoMIV GCCGGC 1 cut(s) 419
NheI GCTAGC 2 cut(s) 457, 1099
NlaIII CATG 6 cut(s) 259, 364, 929, 953, 977, 1211
NlaIV GGNNCC 4 cut(s) 73, 166, 385, 645
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 3 cut(s) 364, 929, 977
NspV TTCGAA 1 cut(s) 545
PctI GAATGC 1 cut(s) 470
PdiI GCCGGC 1 cut(s) 421
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 2 cut(s) 305, 536
Ple19I CGATCG 1 cut(s) 335
PleI GAGTC 1 cut(s) 331
PluTI GGCGCC 2 cut(s) 168, 387
PpsI GAGTC 1 cut(s) 331
Psp124BI GAGCTC 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 4 cut(s) 73, 166, 385, 645
PspPI GGNCC 4 cut(s) 72, 450, 776, 1108
PstI CTGCAG 2 cut(s) 442, 1052
PsuI RGATCY 1 cut(s) 1044
PvuI CGATCG 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 298
RgaI GCGATCGC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 507
SacI GAGCTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 734
Sau3AI GATC 6 cut(s) 332, 478, 523, 739, 770, 1044
Sau96I GGNCC 4 cut(s) 72, 450, 776, 1108
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 454, 1198
SduI GDGCHC 1 cut(s) 218
SfaAI GCGATCGC 1 cut(s) 335
SfaNI GCATC 5 cut(s) 502, 524, 704, 865, 871
SfcI CTRYAG 3 cut(s) 438, 843, 1048
SfoI GGCGCC 2 cut(s) 166, 385
SfuI TTCGAA 1 cut(s) 545
SgfI GCGATCGC 1 cut(s) 335
SinI GGWCC 4 cut(s) 72, 450, 776, 1108
SmiMI CAYNNNNRTG 1 cut(s) 507
SmlI CTYRAG 3 cut(s) 129, 464, 564
SmoI CTYRAG 3 cut(s) 129, 464, 564
Sse9I AATT 3 cut(s) 343, 942, 1229
SspDI GGCGCC 2 cut(s) 164, 383
SspMI CTAG 2 cut(s) 458, 1100
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 452, 1196
TaaI ACNGT 1 cut(s) 1128
TaqI TCGA 4 cut(s) 180, 273, 545, 1009
TasI AATT 3 cut(s) 343, 942, 1229
TauI GCSGC 8 cut(s) 25, 52, 55, 124, 141, 200, 208, 412
TfiI GAWTC 2 cut(s) 305, 536
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 5 cut(s) 481, 533, 708, 730, 891
TspDTI ATGAA 4 cut(s) 549, 684, 840, 988
TspGWI ACGGA 2 cut(s) 274, 983
TspRI CASTG 5 cut(s) 481, 533, 708, 730, 891
VpaK11BI GGWCC 4 cut(s) 72, 450, 776, 1108
XagI CCTNNNNNAGG 1 cut(s) 242
XceI RCATGY 3 cut(s) 364, 929, 977
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 2 cut(s) 458, 1100
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.