AT3G10280

3-ketoacyl-coa synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012744
Physical Location & Seq
Forward (+)
3180064 .. 3181611
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10280.1

Sequence Viewer

Length: 1380 bp
ATGTTCATTGCAATGGCAGATTTCAAGCTTCTTTTGCTTATACTCATCCTTCTATCTCTCTTTGAACTTGATCTACTTCACTTTCACCATGACTTCTTCTCTCCTTTTCCGGTCAAGATCGGTTTACTCCTGATATCCATCTTCTTCTATGCCTACTCAACCACCCGGTCTAAACCGGTTTACCTAGTGGATTTCTCATGTCACCAACCCACGGATTCTTGCAAAATCTCATCCGAAACGTTCTTCAACATGGCCAAAGGCGCTCAACTCTACACCGAAGAAACAATCCAATTCATGACGAGGATACTAAACCGGTCCGGTTTAGGAGACGATACGTACTCCCCACGTTGCATGTTAACCTCTCCACCAACTCCATCAATGTACGAGGCGAGACATGAATCCGAATTAGTCATCTTCGGAGCTCTTAACTCACTATTTAAGAAAACCGGAATCGAACCGAGAGAAGTCGGAATCTTCATCGTAAACTGCAGCTTGTTTAACCCTAACCCATCTCTCTCGTCCATGATCGTAAACCGGTACAAGCTTAAAACCGACGTCAAAACTTACAATCTCTCCGGAATCTCCGTGGACCTCGCCACAAATCTACTAAAGGCAAACCCTAATACCTACGCAGTTATTGTTAGTACGGAGAACATGACTCTAAGCATGTATCGAGGCAACGACCGGTCAATGTTAGTCCCTAACTGTCTATTCCGAGTAGGTGGCGCTGCGGTTATGCTCTCAAACCGGAGTCAAGACCGAGTCCGGTCTAAGTACGAATTGACTCATATTGTAAGGACTCACAAAGGCTCTAGCGATAAACACTACACTTGTGCTGAACAAAAAGAAGATAGTAAAGGTATCGTTGGAGTCGCACTCTCTAAAGAACTAACGGTTGTTGCTGGTGACACGTTAAAGACGAATCTAACAGCACTTGGTCCGCTTGTTTTGCCTTTATCTGAAAAACTCCGGTTTATCCTCTTTTTGGTAAAGAGTAAGCTTTTCCGGTTAAAGGTTAGTCCATATGTTCCGGATTTTAAACTATGTTTCAAGCATTTCTGTATTCACGCTGGTGGTAGAGCGTTACTTGACGCGGTTGAAAAAGGTCTTGGACTTAGTGAGTTTGATCTTGAGCCGTCTCGGATGACTTTGCACCGCTTTGGAAACACTTCAAGCTCGTCGCTGTGGTACGAGCTGGCTTACGTGGAGGCTAAATGTCGGGTCAAACGAGGAGATAGGGTTTGGCAGTTGGCGTTTGGGTCGGGTTTTAAATGTAATAGTATTGTGTGGAGAGCATTGAGAACGATTCCGGCTAACGAGTCATTGGTGGGTAACCCGTGGGGTGACTCGGTTCATAAGTATCCAGTTCACGTGACCTAA

Protein Analysis

459

Amino Acids

51.63

Weight (kDa)

9.18

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 53 - 334 3.9e-126 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 351 - 431 3.2e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 353 - 432 1.1e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 558
AccII CGCG 1 cut(s) 1094
AccIII TCCGGA 2 cut(s) 575, 1030
AciI CCGC 4 cut(s) 731, 941, 1094, 1156
AclI AACGTT 1 cut(s) 239
AcoI YGGCCR 1 cut(s) 252
AcvI CACGTG 1 cut(s) 1372
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 6 cut(s) 338, 383, 539, 646, 776, 1190
AfiI CCNNNNNNNGG 3 cut(s) 211, 985, 1012
AflIII ACRYGT 1 cut(s) 909
AgeI ACCGGT 4 cut(s) 175, 312, 534, 684
AgsI TTSAA 6 cut(s) 25, 65, 247, 1051, 1100, 1173
AjuI GAANNNNNNNTTGG 2 cut(s) 1092, 1124
AleI CACNNNNGTG 1 cut(s) 1071
AluBI AGCT 7 cut(s) 28, 422, 492, 544, 1000, 1176, 1195
AluI AGCT 7 cut(s) 28, 422, 492, 544, 1000, 1176, 1195
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 3 cut(s) 321, 385, 1143
Aor13HI TCCGGA 2 cut(s) 575, 1030
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 2 cut(s) 489, 728
AsiGI ACCGGT 4 cut(s) 175, 312, 534, 684
Asp700I GAANNNNTTC 1 cut(s) 1168
AspLEI GCGC 2 cut(s) 263, 728
AspS9I GGNCC 3 cut(s) 315, 589, 938
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 4 cut(s) 77, 194, 917, 1355
AvaII GGWCC 3 cut(s) 315, 589, 938
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 1 cut(s) 424
BbrPI CACGTG 1 cut(s) 1372
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 2 cut(s) 501, 715
BccI CCATC 3 cut(s) 146, 382, 517
BceAI ACGGC 1 cut(s) 1120
BciVI GTATCC 2 cut(s) 297, 1371
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 321, 385, 1143
BfaI CTAG 2 cut(s) 185, 813
BfmI CTRYAG 1 cut(s) 487
BfoI RGCGCY 2 cut(s) 264, 729
BfuI GTATCC 2 cut(s) 297, 1371
BisI GCNGC 2 cut(s) 490, 729
BlsI GCNGC 2 cut(s) 491, 730
Bme1390I CCNGG 1 cut(s) 166
Bme18I GGWCC 3 cut(s) 315, 589, 938
BmgT120I GGNCC 3 cut(s) 315, 589, 938
BmrFI CCNGG 1 cut(s) 166
BplI GAGNNNNNCTC 2 cut(s) 861, 893
BpuEI CTTGAG 1 cut(s) 1151
BpuMI CCSGG 1 cut(s) 166
BsaAI YACGTR 3 cut(s) 336, 1204, 1372
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 555
BsaJI CCNNGG 3 cut(s) 210, 585, 1337
BsaXI ACNNNNNCTCC 4 cut(s) 557, 587, 1224, 1254
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 985, 1012
Bse118I RCCGGY 4 cut(s) 175, 312, 534, 684
Bse1I ACTGG 1 cut(s) 1364
Bse3DI GCAATG 2 cut(s) 6, 18
Bse8I GATNNNNATC 1 cut(s) 137
BseAI TCCGGA 2 cut(s) 575, 1030
BseDI CCNNGG 3 cut(s) 210, 585, 1337
BseGI GGATG 3 cut(s) 45, 230, 1149
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 3 cut(s) 211, 985, 1012
BseMI GCAATG 2 cut(s) 6, 18
BseNI ACTGG 1 cut(s) 1364
BseRI GAGGAG 1 cut(s) 1245
BseXI GCAGC 2 cut(s) 501, 715
Bsh1236I CGCG 1 cut(s) 1094
Bsh1285I CGRYCG 1 cut(s) 685
BshFI GGCC 1 cut(s) 254
BshTI ACCGGT 4 cut(s) 175, 312, 534, 684
BsiEI CGRYCG 1 cut(s) 685
BsiHKAI GWGCWC 1 cut(s) 424
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 3 cut(s) 211, 985, 1012
BsmAI GTCTC 3 cut(s) 321, 385, 1143
BsmBI CGTCTC 2 cut(s) 321, 1143
BsmFI GGGAC 1 cut(s) 683
BsnI GGCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 424
Bsp13I TCCGGA 2 cut(s) 575, 1030
Bsp143I GATC 4 cut(s) 70, 117, 525, 1126
BspACI CCGC 4 cut(s) 731, 941, 1094, 1156
BspANI GGCC 1 cut(s) 254
BspEI TCCGGA 2 cut(s) 575, 1030
BspFNI CGCG 1 cut(s) 1094
BspHI TCATGA 1 cut(s) 294
BspMAI CTGCAG 1 cut(s) 491
BsrDI GCAATG 2 cut(s) 6, 18
BsrFI RCCGGY 4 cut(s) 175, 312, 534, 684
BsrI ACTGG 1 cut(s) 1364
BssAI RCCGGY 4 cut(s) 175, 312, 534, 684
BssECI CCNNGG 3 cut(s) 210, 585, 1337
BssMI GATC 4 cut(s) 70, 117, 525, 1126
BssNI GRCGYC 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 707, 895
BstACI GRCGYC 1 cut(s) 555
BstBAI YACGTR 3 cut(s) 336, 1204, 1372
BstC8I GCNNGC 1 cut(s) 1197
BstDEI CTNAG 3 cut(s) 662, 771, 1115
BstDSI CCRYGG 3 cut(s) 210, 585, 1337
BstEII GGTNACC 1 cut(s) 1331
BstF5I GGATG 3 cut(s) 45, 230, 1149
BstFNI CGCG 1 cut(s) 1094
BstH2I RGCGCY 2 cut(s) 264, 729
BstHHI GCGC 2 cut(s) 263, 728
BstKTI GATC 4 cut(s) 73, 120, 528, 1129
BstMAI GTCTC 3 cut(s) 321, 385, 1143
BstMBI GATC 4 cut(s) 70, 117, 525, 1126
BstMCI CGRYCG 1 cut(s) 685
BstMWI GCNNNNNNNGC 3 cut(s) 34, 260, 949
BstNSI RCATGY 2 cut(s) 355, 670
BstPI GGTNACC 1 cut(s) 1331
BstSCI CCNGG 1 cut(s) 164
BstSFI CTRYAG 1 cut(s) 487
BstSNI TACGTA 1 cut(s) 336
BstUI CGCG 1 cut(s) 1094
BstV1I GCAGC 2 cut(s) 501, 715
BsuI GTATCC 2 cut(s) 297, 1371
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 3 cut(s) 210, 585, 1337
BtsCI GGATG 3 cut(s) 45, 230, 1149
Cac8I GCNNGC 1 cut(s) 1197
CciI TCATGA 1 cut(s) 294
CfoI GCGC 2 cut(s) 263, 728
Cfr10I RCCGGY 4 cut(s) 175, 312, 534, 684
Cfr13I GGNCC 3 cut(s) 315, 589, 938
CpoI CGGWCCG 1 cut(s) 315
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 6 cut(s) 337, 382, 538, 645, 775, 1189
CspAI ACCGGT 4 cut(s) 175, 312, 534, 684
CspI CGGWCCG 1 cut(s) 315
CviAII CATG 9 cut(s) 89, 198, 250, 295, 352, 395, 523, 655, 667
CviQI GTAC 6 cut(s) 337, 382, 538, 645, 775, 1189
DdeI CTNAG 3 cut(s) 662, 771, 1115
DpnI GATC 4 cut(s) 72, 119, 527, 1128
DpnII GATC 4 cut(s) 70, 117, 525, 1126
DraI TTTAAA 2 cut(s) 1039, 1270
EaeI YGGCCR 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 422
Eco105I TACGTA 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 424
Eco32I GATATC 1 cut(s) 135
Eco47I GGWCC 3 cut(s) 315, 589, 938
Eco53kI GAGCTC 1 cut(s) 422
Eco72I CACGTG 1 cut(s) 1372
Eco91I GGTNACC 1 cut(s) 1331
EcoICRI GAGCTC 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 1331
EcoRV GATATC 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 424
Esp3I CGTCTC 2 cut(s) 321, 1143
FaeI CATG 9 cut(s) 92, 201, 253, 298, 355, 398, 526, 658, 670
FaqI GGGAC 1 cut(s) 683
FatI CATG 9 cut(s) 88, 197, 249, 294, 351, 394, 522, 654, 666
FauNDI CATATG 1 cut(s) 1024
Fnu4HI GCNGC 2 cut(s) 490, 729
FokI GGATG 3 cut(s) 32, 217, 1156
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 2 cut(s) 490, 729
FspBI CTAG 2 cut(s) 185, 813
GlaI GCGC 2 cut(s) 262, 727
GluI GCNGC 2 cut(s) 490, 729
HaeII RGCGCY 2 cut(s) 264, 729
HaeIII GGCC 1 cut(s) 254
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 2 cut(s) 263, 728
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 9 cut(s) 92, 201, 253, 298, 355, 398, 526, 658, 670
Hin6I GCGC 2 cut(s) 261, 726
HinP1I GCGC 2 cut(s) 261, 726
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 3 cut(s) 26, 542, 998
HpaI GTTAAC 1 cut(s) 357
HphI GGTGA 4 cut(s) 77, 194, 917, 1355
Hpy166II GTNNAC 7 cut(s) 125, 181, 357, 484, 532, 589, 1369
Hpy188I TCNGA 7 cut(s) 235, 403, 419, 470, 716, 961, 1143
Hpy188III TCNNGA 7 cut(s) 115, 130, 295, 576, 755, 1031, 1130
Hpy8I GTNNAC 7 cut(s) 125, 181, 357, 484, 532, 589, 1369
Hpy99I CGWCG 2 cut(s) 557, 1183
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 707, 895
HpyCH4IV ACGT 7 cut(s) 239, 335, 346, 555, 911, 1203, 1371
HpyCH4V TGCA 5 cut(s) 11, 222, 351, 489, 1153
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 260, 949
HpyF3I CTNAG 3 cut(s) 662, 771, 1115
HpySE526I ACGT 7 cut(s) 239, 335, 346, 555, 911, 1203, 1371
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 9 cut(s) 92, 201, 253, 298, 355, 398, 526, 658, 670
HspAI GCGC 2 cut(s) 261, 726
Kpn2I TCCGGA 2 cut(s) 575, 1030
KspAI GTTAAC 1 cut(s) 357
Kzo9I GATC 4 cut(s) 70, 117, 525, 1126
LmnI GCTCC 1 cut(s) 419
Lsp1109I GCAGC 2 cut(s) 501, 715
MaeI CTAG 2 cut(s) 185, 813
MaeII ACGT 7 cut(s) 239, 335, 346, 555, 911, 1203, 1371
MaeIII GTNAC 6 cut(s) 200, 905, 1083, 1331, 1343, 1372
MalI GATC 4 cut(s) 72, 119, 527, 1128
MboI GATC 4 cut(s) 70, 117, 525, 1126
MboII GAAGA 8 cut(s) 88, 133, 136, 235, 290, 406, 466, 860
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 3 cut(s) 290, 404, 779
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 8 cut(s) 652, 760, 771, 778, 793, 879, 1328, 1340
MmeI TCCRAC 2 cut(s) 448, 847
MnlI CCTC 8 cut(s) 294, 370, 379, 602, 668, 989, 1201, 1223
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 2 cut(s) 575, 1030
MroXI GAANNNNTTC 1 cut(s) 1168
MscI TGGCCA 1 cut(s) 254
MseI TTAA 9 cut(s) 356, 426, 438, 498, 546, 914, 1010, 1038, 1269
MslI CAYNNNNRTG 2 cut(s) 11, 1071
Msp20I TGGCCA 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 166
MvnI CGCG 1 cut(s) 1094
MwoI GCNNNNNNNGC 3 cut(s) 34, 260, 949
NciI CCSGG 1 cut(s) 166
NdeI CATATG 1 cut(s) 1024
NdeII GATC 4 cut(s) 70, 117, 525, 1126
NlaIII CATG 9 cut(s) 92, 201, 253, 298, 355, 398, 526, 658, 670
NmuCI GTSAC 4 cut(s) 200, 905, 1343, 1372
NspI RCATGY 2 cut(s) 355, 670
OliI CACNNNNGTG 1 cut(s) 1071
PagI TCATGA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 2 cut(s) 917, 1225
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 7 cut(s) 215, 398, 450, 471, 579, 922, 1306
PflFI GACNNNGTC 1 cut(s) 761
PinAI ACCGGT 4 cut(s) 175, 312, 534, 684
PkrI GCNGC 2 cut(s) 491, 730
PleI GAGTC 8 cut(s) 652, 759, 770, 778, 793, 878, 1327, 1340
PmaCI CACGTG 1 cut(s) 1372
PmlI CACGTG 1 cut(s) 1372
PpsI GAGTC 8 cut(s) 652, 759, 770, 778, 793, 878, 1327, 1340
Ppu21I YACGTR 3 cut(s) 336, 1204, 1372
Psp124BI GAGCTC 1 cut(s) 424
Psp1406I AACGTT 1 cut(s) 239
PspCI CACGTG 1 cut(s) 1372
PspEI GGTNACC 1 cut(s) 1331
PspPI GGNCC 3 cut(s) 315, 589, 938
PsrI GAACNNNNNNTAC 2 cut(s) 57, 89
PstI CTGCAG 1 cut(s) 491
PsyI GACNNNGTC 1 cut(s) 761
RsaI GTAC 6 cut(s) 338, 383, 539, 646, 776, 1190
RsaNI GTAC 6 cut(s) 337, 382, 538, 645, 775, 1189
RseI CAYNNNNRTG 2 cut(s) 11, 1071
Rsr2I CGGWCCG 1 cut(s) 315
RsrII CGGWCCG 1 cut(s) 315
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 9 cut(s) 356, 426, 438, 498, 546, 914, 1010, 1038, 1269
SatI GCNGC 2 cut(s) 490, 729
Sau3AI GATC 4 cut(s) 70, 117, 525, 1126
Sau96I GGNCC 3 cut(s) 315, 589, 938
SchI GAGTC 8 cut(s) 652, 760, 771, 778, 793, 879, 1328, 1340
ScrFI CCNGG 1 cut(s) 166
SduI GDGCHC 1 cut(s) 424
SfcI CTRYAG 1 cut(s) 487
SinI GGWCC 3 cut(s) 315, 589, 938
SmiMI CAYNNNNRTG 2 cut(s) 11, 1071
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
SnaBI TACGTA 1 cut(s) 336
Sse9I AATT 3 cut(s) 290, 404, 779
SsiI CCGC 4 cut(s) 731, 941, 1094, 1156
SspMI CTAG 2 cut(s) 185, 813
SstI GAGCTC 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 707, 895
TaiI ACGT 7 cut(s) 242, 338, 349, 558, 914, 1206, 1374
TaqI TCGA 2 cut(s) 453, 673
TaqII GACCGA 1 cut(s) 774
TasI AATT 3 cut(s) 290, 404, 779
TfiI GAWTC 7 cut(s) 215, 398, 450, 471, 579, 922, 1306
Tru1I TTAA 9 cut(s) 356, 426, 438, 498, 546, 914, 1010, 1038, 1269
Tru9I TTAA 9 cut(s) 356, 426, 438, 498, 546, 914, 1010, 1038, 1269
TseFI GTSAC 4 cut(s) 200, 905, 1343, 1372
TseI GCWGC 2 cut(s) 489, 728
Tsp45I GTSAC 4 cut(s) 200, 905, 1343, 1372
TspDTI ATGAA 4 cut(s) 283, 411, 466, 1343
TspGWI ACGGA 3 cut(s) 227, 574, 662
Tth111I GACNNNGTC 1 cut(s) 761
VpaK11BI GGWCC 3 cut(s) 315, 589, 938
XceI RCATGY 2 cut(s) 355, 670
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 2 cut(s) 185, 813
ZraI GACGTC 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.