Rh1DG397400

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012744
Physical Location & Seq
Forward (+)
61658739 .. 61660208
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG397400.1

Sequence Viewer

Length: 1470 bp
ATGGCACCTCTAGTTTCTGAGCAATGCCTCAACTCCACCACCCCTTCCACTTTTTTATCAAAGCAGATTCTTTCAACTCTCTCCCAAAGTCTAGGACTTTTGGTGCTCATACTTGTAACATCCCTTGAAGCCTTGTTTCTGCTCCAAAAAAGACCACCCATTTTTCACTTCATTAACCTCTCTTGCTTTACCATCGTCTTCATCCTCAAGCCTTACCTCTCCAAAAAGACTCATAAAGTCTACTTGGTAGACTTCTCATGTCTAAAACCACCAAGCTCCTGCAAAGTCCCCTTCTCCAAATTCCTCAAGAACTCCTCCATGATTGAAGGTTTCGACAGTGAAAGCATATCTTTCATGGCCAACATCCTCACTTCTTCGGGCCAAAGCCAAGAGACCTATCTCCCTCCAGCCTTACACCACATTCCTCCCAAAACCCACCACCATGAATACATCAAAGAAGTTCACATGGTCCTTTTCCCCATCATGGATGACCTTTTGGCCAAAACCAAACTCTCCCCTCAAGACATTGACATCCTAATAGTAAATTGTAGCGGTTTCAATCCCTCTCCTTCTCTAGCCTCCATTGTCATTAACCACTACTCCATGAGAACTGACATCAAGAGCTTCAACCTCTCGGGAATGGGATGCAGCGCAGGCGCTTTGGGCATTGATCTGGCCCAAAATCTCCTCCAAATTCACAGGAACTCATACGCCGTTGTTCTCAGCACAGAGATTTTATCGACAGGTTGGTACGCCGGCCACGAAAAACCCAAGTTGATCCTCAACTGCCTCTTCAGAATGGGCAGCGCAGCCATTTTGCTCACAAACAAAAATCAAACATCCAAATCTCCAAAGTACGAACTGTTCAAAACACTCAGAACCCAGAAAGCCTACGACGACAGAGCTTACCTCTCCACCATTCGAGAGGAAGACTCCGACGGAAAACTCGGCGTCACCATCAAACGTGATCTGCTACAAGTCGCCGGCGAGACGCTCCGAGCAAACGTCACCGTTTTGGGGTCGTCAATCCTTCCATTATCTGAGAAATTCTGGCACGGTGTTTCGATAATCAGGAAGAGATTCTTAAACAAGTCTGAGGAGATTTACGTTCCGAATTTCAAGAGAGTGATAGGGCATTTTTGTCTGCCGGCGTCGGGGAGACCGGTAATAAGGGAAATCGGGAAAGGTTTGAAGCTTGGAGATAGGGAAATGGAGGCTGCTGTGATGACGTTGCACAGGTTTGGGAACCAGTCGTCTTCTTCGTTGTGGTATGAATTGGCGTACTTGGAGGGAAAAGAGAGGGTGAAGAAAGGTGACAAGGTTTGGCAGCTTGGGATGGGGACTGGGCCGAAGTGCACTAGTTTGGTTTGGAAGTGTATAAGGCCCATTGAGGGTGAGTCCAAGAAAGGCCCGTGGGCCGATTGCATCCATCGGTATCCATTTGAAGCCCACGGTGAAGGAGCTAATTAG

Protein Analysis

489

Amino Acids

54.62

Weight (kDa)

9.21

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 73 - 360 2.1e-109 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 377 - 458 8.8e-08 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 240, 249
AciI CCGC 1 cut(s) 552
AclWI GGATC 1 cut(s) 772
AcoI YGGCCR 3 cut(s) 357, 498, 757
AcsI RAATTY 4 cut(s) 299, 693, 1046, 1114
AcuI CTGAAG 1 cut(s) 778
AcyI GRCGYC 2 cut(s) 951, 1151
AfaI GTAC 3 cut(s) 752, 857, 1283
AfiI CCNNNNNNNGG 4 cut(s) 484, 1017, 1154, 1391
AgeI ACCGGT 1 cut(s) 1162
AgsI TTSAA 9 cut(s) 75, 128, 326, 559, 628, 868, 1120, 1192, 1445
AhlI ACTAGT 1 cut(s) 1358
AluBI AGCT 6 cut(s) 276, 624, 905, 1195, 1330, 1463
AluI AGCT 6 cut(s) 276, 624, 905, 1195, 1330, 1463
Alw21I GWGCWC 2 cut(s) 108, 1358
Alw26I GTCTC 3 cut(s) 386, 983, 1153
Alw44I GTGCAC 1 cut(s) 1354
AlwI GGATC 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 9 cut(s) 357, 379, 498, 675, 757, 1346, 1382, 1408, 1416
ApaLI GTGCAC 1 cut(s) 1354
ApeKI GCWGC 5 cut(s) 648, 804, 809, 1217, 1327
ApoI RAATTY 4 cut(s) 299, 693, 1046, 1114
AsiGI ACCGGT 1 cut(s) 1162
Asp700I GAANNNNTTC 1 cut(s) 1079
AspLEI GCGC 3 cut(s) 653, 659, 809
AspS9I GGNCC 7 cut(s) 379, 469, 676, 1346, 1383, 1409, 1416
AsuHPI GGTGA 6 cut(s) 946, 1000, 1315, 1325, 1406, 1466
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1358
BalI TGGCCA 2 cut(s) 359, 500
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 3 cut(s) 190, 936, 1248
Bbv12I GWGCWC 2 cut(s) 108, 1358
BbvI GCAGC 5 cut(s) 660, 816, 821, 1204, 1339
BccI CCATC 5 cut(s) 200, 488, 965, 1330, 1437
BceAI ACGGC 1 cut(s) 698
BciVI GTATCC 1 cut(s) 1446
BcoDI GTCTC 3 cut(s) 386, 983, 1153
BcuI ACTAGT 1 cut(s) 1358
BfaI CTAG 4 cut(s) 11, 92, 575, 1359
BfoI RGCGCY 1 cut(s) 660
BfuI GTATCC 1 cut(s) 1446
BisI GCNGC 5 cut(s) 649, 805, 810, 1218, 1328
BlsI GCNGC 5 cut(s) 650, 806, 811, 1219, 1329
Bme18I GGWCC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 7 cut(s) 379, 469, 676, 1346, 1383, 1409, 1416
BmiI GGNNCC 2 cut(s) 6, 1247
BmrI ACTGGG 1 cut(s) 1353
BmsI GCATC 2 cut(s) 635, 1434
BmuI ACTGGG 1 cut(s) 1353
BpiI GAAGAC 3 cut(s) 190, 936, 1248
BplI GAGNNNNNCTC 4 cut(s) 894, 926, 917, 949
BpmI CTGGAG 1 cut(s) 390
BpuEI CTTGAG 3 cut(s) 191, 290, 504
BsaHI GRCGYC 2 cut(s) 951, 1151
BsaI GGTCTC 2 cut(s) 386, 1153
BsaJI CCNNGG 2 cut(s) 1412, 1450
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 2 cut(s) 584, 614
Bsc4I CCNNNNNNNGG 4 cut(s) 484, 1017, 1154, 1391
Bse118I RCCGGY 4 cut(s) 755, 983, 1147, 1162
Bse1I ACTGG 2 cut(s) 1249, 1348
Bse3DI GCAATG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 1412, 1450
BseGI GGATG 9 cut(s) 119, 201, 363, 493, 531, 650, 839, 1341, 1425
BseLI CCNNNNNNNGG 4 cut(s) 484, 1017, 1154, 1391
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 5 cut(s) 9, 736, 889, 1032, 1086
BseNI ACTGG 2 cut(s) 1249, 1348
BseRI GAGGAG 3 cut(s) 304, 677, 1112
BseSI GKGCMC 1 cut(s) 1358
BseXI GCAGC 5 cut(s) 660, 816, 821, 1204, 1339
BshFI GGCC 9 cut(s) 359, 381, 500, 677, 759, 1348, 1384, 1410, 1418
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 1162
BsiHKAI GWGCWC 2 cut(s) 108, 1358
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 4 cut(s) 756, 984, 1148, 1163
BslFI GGGAC 2 cut(s) 272, 1354
BslI CCNNNNNNNGG 4 cut(s) 484, 1017, 1154, 1391
BsmAI GTCTC 3 cut(s) 386, 983, 1153
BsmBI CGTCTC 1 cut(s) 983
BsmFI GGGAC 2 cut(s) 272, 1354
BsnI GGCC 9 cut(s) 359, 381, 500, 677, 759, 1348, 1384, 1410, 1418
Bso31I GGTCTC 2 cut(s) 386, 1153
BsoBI CYCGRG 1 cut(s) 634
Bsp1286I GDGCHC 2 cut(s) 108, 1358
Bsp143I GATC 3 cut(s) 670, 777, 967
BspACI CCGC 1 cut(s) 552
BspANI GGCC 9 cut(s) 359, 381, 500, 677, 759, 1348, 1384, 1410, 1418
BspCNI CTCAG 5 cut(s) 10, 735, 888, 1033, 1087
BspLI GGNNCC 2 cut(s) 6, 1247
BspPI GGATC 1 cut(s) 772
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 2 cut(s) 386, 1153
BsrDI GCAATG 1 cut(s) 29
BsrFI RCCGGY 4 cut(s) 755, 983, 1147, 1162
BsrI ACTGG 2 cut(s) 1249, 1348
BssAI RCCGGY 4 cut(s) 755, 983, 1147, 1162
BssECI CCNNGG 2 cut(s) 1412, 1450
BssMI GATC 3 cut(s) 670, 777, 967
BssNI GRCGYC 2 cut(s) 951, 1151
Bst4CI ACNGT 5 cut(s) 338, 864, 1012, 1058, 1454
Bst6I CTCTTC 2 cut(s) 797, 1070
BstACI GRCGYC 2 cut(s) 951, 1151
BstC8I GCNNGC 4 cut(s) 655, 757, 985, 1149
BstDEI CTNAG 5 cut(s) 18, 722, 875, 1041, 1095
BstDSI CCRYGG 2 cut(s) 1412, 1450
BstF5I GGATG 9 cut(s) 119, 201, 363, 493, 531, 650, 839, 1341, 1425
BstH2I RGCGCY 1 cut(s) 660
BstHHI GCGC 3 cut(s) 653, 659, 809
BstKTI GATC 3 cut(s) 673, 780, 970
BstMAI GTCTC 3 cut(s) 386, 983, 1153
BstMBI GATC 3 cut(s) 670, 777, 967
BstMWI GCNNNNNNNGC 2 cut(s) 654, 663
BstSLI GKGCMC 1 cut(s) 1358
BstV1I GCAGC 5 cut(s) 660, 816, 821, 1204, 1339
BstV2I GAAGAC 3 cut(s) 190, 936, 1248
BsuI GTATCC 1 cut(s) 1446
BsuRI GGCC 9 cut(s) 359, 381, 500, 677, 759, 1348, 1384, 1410, 1418
BtgI CCRYGG 2 cut(s) 1412, 1450
BtsCI GGATG 9 cut(s) 119, 201, 363, 493, 531, 650, 839, 1341, 1425
BtsIMutI CAGTG 1 cut(s) 343
Cac8I GCNNGC 4 cut(s) 655, 757, 985, 1149
CfoI GCGC 3 cut(s) 653, 659, 809
Cfr10I RCCGGY 4 cut(s) 755, 983, 1147, 1162
Cfr13I GGNCC 7 cut(s) 379, 469, 676, 1346, 1383, 1409, 1416
CseI GACGC 3 cut(s) 940, 1000, 1140
Csp6I GTAC 3 cut(s) 751, 856, 1282
CspAI ACCGGT 1 cut(s) 1162
CviAII CATG 7 cut(s) 258, 319, 355, 443, 466, 484, 604
CviQI GTAC 3 cut(s) 751, 856, 1282
DdeI CTNAG 5 cut(s) 18, 722, 875, 1041, 1095
DpnI GATC 3 cut(s) 672, 779, 969
DpnII GATC 3 cut(s) 670, 777, 967
EaeI YGGCCR 3 cut(s) 357, 498, 757
Eam1104I CTCTTC 2 cut(s) 797, 1070
EarI CTCTTC 2 cut(s) 797, 1070
Eco31I GGTCTC 2 cut(s) 386, 1153
Eco47I GGWCC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 778
Eco88I CYCGRG 1 cut(s) 634
Esp3I CGTCTC 1 cut(s) 983
FaeI CATG 7 cut(s) 261, 322, 358, 446, 469, 487, 607
FalI AAGNNNNNCTT 4 cut(s) 334, 366, 1067, 1099
FaqI GGGAC 2 cut(s) 272, 1354
FatI CATG 7 cut(s) 257, 318, 354, 442, 465, 483, 603
FblI GTMKAC 2 cut(s) 240, 249
Fnu4HI GCNGC 5 cut(s) 649, 805, 810, 1218, 1328
FokI GGATG 9 cut(s) 106, 188, 350, 500, 518, 657, 826, 1348, 1412
Fsp4HI GCNGC 5 cut(s) 649, 805, 810, 1218, 1328
FspBI CTAG 4 cut(s) 11, 92, 575, 1359
GlaI GCGC 3 cut(s) 652, 658, 808
GluI GCNGC 5 cut(s) 649, 805, 810, 1218, 1328
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 660
HaeIII GGCC 9 cut(s) 359, 381, 500, 677, 759, 1348, 1384, 1410, 1418
HapII CCGG 4 cut(s) 756, 984, 1148, 1163
HgaI GACGC 3 cut(s) 940, 1000, 1140
HhaI GCGC 3 cut(s) 653, 659, 809
Hin1I GRCGYC 2 cut(s) 951, 1151
Hin1II CATG 7 cut(s) 261, 322, 358, 446, 469, 487, 607
Hin6I GCGC 3 cut(s) 651, 657, 807
HinP1I GCGC 3 cut(s) 651, 657, 807
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 5 cut(s) 67, 229, 932, 1080, 1397
HpaII CCGG 4 cut(s) 756, 984, 1148, 1163
HphI GGTGA 6 cut(s) 946, 1000, 1315, 1325, 1406, 1466
Hpy166II GTNNAC 4 cut(s) 241, 250, 463, 1356
Hpy188I TCNGA 8 cut(s) 19, 797, 878, 937, 998, 1042, 1096, 1113
Hpy188III TCNNGA 8 cut(s) 307, 521, 619, 636, 923, 1072, 1120, 1180
Hpy8I GTNNAC 4 cut(s) 241, 250, 463, 1356
Hpy99I CGWCG 3 cut(s) 899, 941, 1156
HpyAV CCTTC 6 cut(s) 54, 301, 320, 579, 1040, 1451
HpyCH4III ACNGT 5 cut(s) 338, 864, 1012, 1058, 1454
HpyCH4IV ACGT 4 cut(s) 964, 1005, 1107, 1229
HpyCH4V TGCA 5 cut(s) 282, 648, 1234, 1356, 1425
HpyF10VI GCNNNNNNNGC 2 cut(s) 654, 663
HpyF3I CTNAG 5 cut(s) 18, 722, 875, 1041, 1095
HpySE526I ACGT 4 cut(s) 964, 1005, 1107, 1229
Hsp92I GRCGYC 2 cut(s) 951, 1151
Hsp92II CATG 7 cut(s) 261, 322, 358, 446, 469, 487, 607
HspAI GCGC 3 cut(s) 651, 657, 807
KroI GCCGGC 3 cut(s) 755, 983, 1147
KroNI GCCGGC 3 cut(s) 757, 985, 1149
Kzo9I GATC 3 cut(s) 670, 777, 967
LmnI GCTCC 4 cut(s) 147, 281, 999, 1460
Lsp1109I GCAGC 5 cut(s) 660, 816, 821, 1204, 1339
LweI GCATC 2 cut(s) 635, 1434
MaeI CTAG 4 cut(s) 11, 92, 575, 1359
MaeII ACGT 4 cut(s) 964, 1005, 1107, 1229
MaeIII GTNAC 4 cut(s) 115, 952, 1006, 1313
MalI GATC 3 cut(s) 672, 779, 969
MboI GATC 3 cut(s) 670, 777, 967
MboII GAAGA 8 cut(s) 190, 366, 784, 941, 1087, 1248, 1251, 1318
MhlI GDGCHC 2 cut(s) 108, 1358
MlsI TGGCCA 2 cut(s) 359, 500
MluCI AATT 7 cut(s) 299, 544, 693, 1046, 1114, 1274, 1465
MluNI TGGCCA 2 cut(s) 359, 500
MlyI GAGTC 3 cut(s) 223, 926, 1406
MmeI TCCRAC 1 cut(s) 960
Mox20I TGGCCA 2 cut(s) 359, 500
MreI CGCCGGCG 1 cut(s) 983
MroNI GCCGGC 3 cut(s) 755, 983, 1147
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 2 cut(s) 359, 500
MseI TTAA 3 cut(s) 174, 591, 1085
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 2 cut(s) 359, 500
MspI CCGG 4 cut(s) 756, 984, 1148, 1163
MwoI GCNNNNNNNGC 2 cut(s) 654, 663
NaeI GCCGGC 3 cut(s) 757, 985, 1149
NdeII GATC 3 cut(s) 670, 777, 967
NgoMIV GCCGGC 3 cut(s) 755, 983, 1147
NlaIII CATG 7 cut(s) 261, 322, 358, 446, 469, 487, 607
NlaIV GGNNCC 2 cut(s) 6, 1247
NmeAIII GCCGAG 1 cut(s) 927
NmuCI GTSAC 3 cut(s) 952, 1006, 1313
PcsI WCGNNNNNNNCGW 2 cut(s) 759, 1259
PdiI GCCGGC 3 cut(s) 757, 985, 1149
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 2 cut(s) 67, 1080
PinAI ACCGGT 1 cut(s) 1162
PkrI GCNGC 5 cut(s) 650, 806, 811, 1219, 1329
PleI GAGTC 3 cut(s) 223, 926, 1405
PpsI GAGTC 3 cut(s) 223, 926, 1405
PspN4I GGNNCC 2 cut(s) 6, 1247
PspPI GGNCC 7 cut(s) 379, 469, 676, 1346, 1383, 1409, 1416
PsrI GAACNNNNNNTAC 2 cut(s) 848, 880
RsaI GTAC 3 cut(s) 752, 857, 1283
RsaNI GTAC 3 cut(s) 751, 856, 1282
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 3 cut(s) 174, 591, 1085
SatI GCNGC 5 cut(s) 649, 805, 810, 1218, 1328
Sau3AI GATC 3 cut(s) 670, 777, 967
Sau96I GGNCC 7 cut(s) 379, 469, 676, 1346, 1383, 1409, 1416
SchI GAGTC 3 cut(s) 223, 926, 1406
SduI GDGCHC 2 cut(s) 108, 1358
SfaNI GCATC 2 cut(s) 635, 1434
SgrAI CRCCGGYG 1 cut(s) 983
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 3 cut(s) 206, 305, 519
SmoI CTYRAG 3 cut(s) 206, 305, 519
SpeI ACTAGT 1 cut(s) 1358
Sse9I AATT 7 cut(s) 299, 544, 693, 1046, 1114, 1274, 1465
SsiI CCGC 1 cut(s) 552
SspMI CTAG 4 cut(s) 11, 92, 575, 1359
TaaI ACNGT 5 cut(s) 338, 864, 1012, 1058, 1454
TaiI ACGT 4 cut(s) 967, 1008, 1110, 1232
TaqI TCGA 4 cut(s) 333, 740, 922, 1064
TasI AATT 7 cut(s) 299, 544, 693, 1046, 1114, 1274, 1465
TfiI GAWTC 2 cut(s) 67, 1080
Tru1I TTAA 3 cut(s) 174, 591, 1085
Tru9I TTAA 3 cut(s) 174, 591, 1085
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 3 cut(s) 952, 1006, 1313
TseI GCWGC 5 cut(s) 648, 804, 809, 1217, 1327
Tsp45I GTSAC 3 cut(s) 952, 1006, 1313
TspDTI ATGAA 5 cut(s) 160, 190, 343, 459, 1287
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 343
VneI GTGCAC 1 cut(s) 1354
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 4 cut(s) 299, 693, 1046, 1114
XmiI GTMKAC 2 cut(s) 240, 249
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 4 cut(s) 11, 92, 575, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.