MD01G1188700.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012744
Physical Location & Seq
Forward (+)
28705813 .. 28707637
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1188700.v1.1.491

Sequence Viewer

Length: 1383 bp
ATGGAACTTCAAAGACTTTTGCTCCTCATAATTGTAACTTCAGTGGAAACCCTAATTGTCCTGCAAAAAAGAGAACCCATTTTCCACTTCATCCATCTCCCATGCCTGATCATTTTCTTCACCCTCCAGTCTTGCTTCTTCTCAAACCCTACCCAAGTCTATTTGGTGGACTTTTCATGCTTAAAACCACCAAGCTCCTGCATTGTTCCCTTCTCCAAATTCCTCGGAAACGCCTCAAAGTTCGAATCTTTCGACAAGGAAAGCCTCGCTTTCATGGCGAAAGTGCTCGCTTCTTCAGGTCACGGCCAAGAAACCTACCTTCCTCCAGCCCTACACCACATTCCACCATTGTCCCACCACCAAGAATCCATCGAAGAAGTCCACATGGTGCTCTTTCCCATCATGGATGATCTTTTGGCCAAAACAAACCTTTCTCCGCAAGAAATTGACATTTTGATAGTGAACTGCAGCGGCTTCTGCCCTTCTCCTTCTTTCGCCTCCATTGTCATCAACAAGTACTCGATGCGAAGCGACGTAAAGAGCTACAGTCTCTCTGGCATGGGGTGCAGCGCAAGCGCTTTAGCCATTGATTTGGCTAAAAATCTTCTGAAAGTTCACAAGAATTCTTATGCCGTTGTTCTTAGCACGGAGATTTTATCAACGGGCTGGTATGCAGGACACGAGCAGCCTAAGTTGATCCTCAATTGCGTCTTCCGAATGGGGAGCGCGGCAATCTTGCTTACGAACAAGAAACGAGAAAAAATGAATTCGAAATACCAACTGTTCCTGACACTAAGAACCCAGAAAGCGTTTGATGATAGGGCTTATCTTTCGACCATACGCGAAGAGGACTCTAATGGAAAACTTGGCGTCACCATCAAGCGAGATATTCTAGAGGTGGCTGGAGAGTTACTTCGTTGGAATGTGATGGTTCTCGGCTCTTCAATCTTGCCATTCTTGGAAAAATTTCGATATGGGGTTTCAGTAATCCGGAAGAGGTACATTGACAAGTCTACAGAGATTTACGTGCCGAATTTCAAGACTGTGATCGAGCATTTTTGTTTGCCGACTTCCGGGAGGCAGTTGGTGAGCGAGCTGGCCAAAGGGTTGAAGCTTGGGGACAGGGAAATGGAGGCTGCTCTGATGACACTGCACAGATTTGGGAACCAGTCATCGTCTTCGCTGTGGTATGAATTGGCATACTTGGAGGCCAAACAAAAAGTGAAGAAAGGTGATAGGGTTTGGCAGCTTGGAATGGGGACTGGGCCAAAATGCACTAGTTTGGTTTGGAAATGCATAAGGTCCATGGCTGAAGATGAGTCCAAGAAGGGCCCATGGGCCGACAGCATCCATCGATATCCGATGGTTGCCATGGATCGATGA

Protein Analysis

461

Amino Acids

51.94

Weight (kDa)

8.94

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 49 - 333 4.7e-106 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 350 - 431 3.8e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1013
AccII CGCG 2 cut(s) 728, 843
AccIII TCCGGA 1 cut(s) 990
AciI CCGC 3 cut(s) 437, 471, 728
AclWI GGATC 1 cut(s) 691
AcoI YGGCCR 3 cut(s) 304, 417, 1098
AcsI RAATTY 5 cut(s) 218, 622, 766, 965, 1033
AcuI CTGAAG 3 cut(s) 24, 279, 1332
AcyI GRCGYC 1 cut(s) 870
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 2 cut(s) 518, 1001
AfeI AGCGCT 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 1073
AgsI TTSAA 4 cut(s) 11, 945, 1039, 1111
AhlI ACTAGT 1 cut(s) 1277
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
AluBI AGCT 5 cut(s) 195, 543, 1096, 1114, 1249
AluI AGCT 5 cut(s) 195, 543, 1096, 1114, 1249
Alw21I GWGCWC 2 cut(s) 288, 393
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 691
Aor13HI TCCGGA 1 cut(s) 990
Aor51HI AGCGCT 1 cut(s) 577
AoxI GGCC 7 cut(s) 304, 417, 1098, 1209, 1265, 1330, 1338
ApaI GGGCCC 1 cut(s) 1334
ApeKI GCWGC 5 cut(s) 468, 567, 685, 1136, 1246
ApoI RAATTY 5 cut(s) 218, 622, 766, 965, 1033
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 572, 578, 728
AspS9I GGNCC 5 cut(s) 1265, 1302, 1330, 1331, 1338
AsuC2I CCSGG 1 cut(s) 1075
AsuHPI GGTGA 4 cut(s) 112, 865, 1099, 1244
AsuII TTCGAA 2 cut(s) 243, 770
AvaII GGWCC 1 cut(s) 1302
BaeGI GKGCMC 1 cut(s) 1334
BalI TGGCCA 2 cut(s) 419, 1100
BanII GRGCYC 1 cut(s) 1334
BauI CACGAG 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 703, 1170
Bbv12I GWGCWC 2 cut(s) 288, 393
BbvI GCAGC 5 cut(s) 480, 579, 697, 1123, 1258
BccI CCATC 7 cut(s) 102, 377, 407, 884, 922, 1357, 1359
BceAI ACGGC 2 cut(s) 319, 617
BclI TGATCA 1 cut(s) 108
BcnI CCSGG 1 cut(s) 1075
BcoDI GTCTC 1 cut(s) 554
BcuI ACTAGT 1 cut(s) 1277
BfaI CTAG 2 cut(s) 893, 1278
BfmI CTRYAG 3 cut(s) 466, 544, 1014
BfoI RGCGCY 1 cut(s) 579
BisI GCNGC 7 cut(s) 469, 472, 568, 686, 729, 1137, 1247
BlsI GCNGC 7 cut(s) 470, 473, 569, 687, 730, 1138, 1248
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 1 cut(s) 1075
Bme18I GGWCC 1 cut(s) 1302
BmgT120I GGNCC 5 cut(s) 1265, 1302, 1330, 1331, 1338
BmiI GGNNCC 2 cut(s) 1166, 1332
BmrFI CCNGG 1 cut(s) 1075
BmrI ACTGGG 1 cut(s) 1272
BmsI GCATC 2 cut(s) 513, 1356
BmuI ACTGGG 1 cut(s) 1272
BpiI GAAGAC 2 cut(s) 703, 1170
BpmI CTGGAG 3 cut(s) 110, 309, 924
Bpu14I TTCGAA 2 cut(s) 243, 770
BpuMI CCSGG 1 cut(s) 1075
Bsa29I ATCGAT 2 cut(s) 1354, 1378
BsaAI YACGTR 1 cut(s) 1027
BsaHI GRCGYC 1 cut(s) 870
BsaJI CCNNGG 4 cut(s) 223, 1305, 1334, 1371
BsaWI WCCGGW 1 cut(s) 990
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 1 cut(s) 1073
Bse1I ACTGG 3 cut(s) 127, 1168, 1267
BseAI TCCGGA 1 cut(s) 990
BseCI ATCGAT 2 cut(s) 1354, 1378
BseDI CCNNGG 4 cut(s) 223, 1305, 1334, 1371
BseGI GGATG 3 cut(s) 90, 412, 1347
BseLI CCNNNNNNNGG 1 cut(s) 1073
BseNI ACTGG 3 cut(s) 127, 1168, 1267
BseRI GAGGAG 1 cut(s) 14
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 5 cut(s) 480, 579, 697, 1123, 1258
BsgI GTGCAG 2 cut(s) 586, 1136
Bsh1236I CGCG 2 cut(s) 728, 843
BshFI GGCC 7 cut(s) 306, 419, 1100, 1211, 1267, 1332, 1340
BshVI ATCGAT 2 cut(s) 1354, 1378
BsiHKAI GWGCWC 2 cut(s) 288, 393
BsiSI CCGG 2 cut(s) 991, 1074
BslFI GGGAC 3 cut(s) 337, 1133, 1273
BslI CCNNNNNNNGG 1 cut(s) 1073
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 3 cut(s) 337, 1133, 1273
BsnI GGCC 7 cut(s) 306, 419, 1100, 1211, 1267, 1332, 1340
Bsp119I TTCGAA 2 cut(s) 243, 770
Bsp120I GGGCCC 1 cut(s) 1330
Bsp1286I GDGCHC 3 cut(s) 288, 393, 1334
Bsp13I TCCGGA 1 cut(s) 990
Bsp143I GATC 5 cut(s) 108, 409, 696, 1047, 1375
Bsp19I CCATGG 3 cut(s) 1305, 1334, 1371
BspACI CCGC 3 cut(s) 437, 471, 728
BspANI GGCC 7 cut(s) 306, 419, 1100, 1211, 1267, 1332, 1340
BspDI ATCGAT 2 cut(s) 1354, 1378
BspEI TCCGGA 1 cut(s) 990
BspFNI CGCG 2 cut(s) 728, 843
BspLI GGNNCC 2 cut(s) 1166, 1332
BspMAI CTGCAG 1 cut(s) 470
BspPI GGATC 1 cut(s) 691
BspQI GCTCTTC 1 cut(s) 946
BspT104I TTCGAA 2 cut(s) 243, 770
BsrI ACTGG 3 cut(s) 127, 1168, 1267
BssECI CCNNGG 4 cut(s) 223, 1305, 1334, 1371
BssMI GATC 5 cut(s) 108, 409, 696, 1047, 1375
BssNI GRCGYC 1 cut(s) 870
BssSI CACGAG 1 cut(s) 680
BssT1I CCWWGG 3 cut(s) 1305, 1334, 1371
Bst2BI CACGAG 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 548, 783, 1045
Bst6I CTCTTC 3 cut(s) 840, 946, 989
BstACI GRCGYC 1 cut(s) 870
BstAPI GCANNNNNTGC 1 cut(s) 564
BstBAI YACGTR 1 cut(s) 1027
BstBI TTCGAA 2 cut(s) 243, 770
BstC8I GCNNGC 4 cut(s) 288, 574, 1094, 1098
BstDEI CTNAG 3 cut(s) 641, 690, 794
BstDSI CCRYGG 3 cut(s) 1305, 1334, 1371
BstF5I GGATG 3 cut(s) 90, 412, 1347
BstFNI CGCG 2 cut(s) 728, 843
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 3 cut(s) 572, 578, 728
BstKTI GATC 5 cut(s) 111, 412, 699, 1050, 1378
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 5 cut(s) 108, 409, 696, 1047, 1375
BstMWI GCNNNNNNNGC 4 cut(s) 275, 477, 564, 573
BstSCI CCNGG 1 cut(s) 1073
BstSFI CTRYAG 3 cut(s) 466, 544, 1014
BstSLI GKGCMC 1 cut(s) 1334
BstUI CGCG 2 cut(s) 728, 843
BstV1I GCAGC 5 cut(s) 480, 579, 697, 1123, 1258
BstV2I GAAGAC 2 cut(s) 703, 1170
BstXI CCANNNNNNTGG 1 cut(s) 592
Bsu15I ATCGAT 2 cut(s) 1354, 1378
BsuRI GGCC 7 cut(s) 306, 419, 1100, 1211, 1267, 1332, 1340
BsuTUI ATCGAT 2 cut(s) 1354, 1378
BtgI CCRYGG 3 cut(s) 1305, 1334, 1371
BtsCI GGATG 3 cut(s) 90, 412, 1347
BtsI GCAGTG 1 cut(s) 1148
BtsIMutI CAGTG 2 cut(s) 48, 1148
Cac8I GCNNGC 4 cut(s) 288, 574, 1094, 1098
CfoI GCGC 3 cut(s) 572, 578, 728
Cfr13I GGNCC 5 cut(s) 1265, 1302, 1330, 1331, 1338
ClaI ATCGAT 2 cut(s) 1354, 1378
CseI GACGC 2 cut(s) 697, 859
Csp6I GTAC 2 cut(s) 517, 1000
CviAII CATG 9 cut(s) 102, 177, 274, 385, 403, 559, 1306, 1335, 1372
CviQI GTAC 2 cut(s) 517, 1000
DdeI CTNAG 3 cut(s) 641, 690, 794
DpnI GATC 5 cut(s) 110, 411, 698, 1049, 1377
DpnII GATC 5 cut(s) 108, 409, 696, 1047, 1375
DraIII CACNNNGTG 1 cut(s) 388
EaeI YGGCCR 3 cut(s) 304, 417, 1098
Eam1104I CTCTTC 3 cut(s) 840, 946, 989
EarI CTCTTC 3 cut(s) 840, 946, 989
Eco130I CCWWGG 3 cut(s) 1305, 1334, 1371
Eco24I GRGCYC 1 cut(s) 1334
Eco32I GATATC 1 cut(s) 1358
Eco47I GGWCC 1 cut(s) 1302
Eco47III AGCGCT 1 cut(s) 577
Eco57I CTGAAG 3 cut(s) 24, 279, 1332
EcoO109I RGGNCCY 1 cut(s) 1330
EcoRI GAATTC 2 cut(s) 622, 766
EcoRV GATATC 1 cut(s) 1358
EcoT14I CCWWGG 3 cut(s) 1305, 1334, 1371
EcoT22I ATGCAT 1 cut(s) 1298
EcoT38I GRGCYC 1 cut(s) 1334
ErhI CCWWGG 3 cut(s) 1305, 1334, 1371
FaeI CATG 9 cut(s) 105, 180, 277, 388, 406, 562, 1309, 1338, 1375
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FaqI GGGAC 3 cut(s) 337, 1133, 1273
FatI CATG 9 cut(s) 101, 176, 273, 384, 402, 558, 1305, 1334, 1371
FbaI TGATCA 1 cut(s) 108
FblI GTMKAC 1 cut(s) 1013
Fnu4HI GCNGC 7 cut(s) 469, 472, 568, 686, 729, 1137, 1247
FokI GGATG 3 cut(s) 77, 419, 1334
FriOI GRGCYC 1 cut(s) 1334
Fsp4HI GCNGC 7 cut(s) 469, 472, 568, 686, 729, 1137, 1247
FspBI CTAG 2 cut(s) 893, 1278
GlaI GCGC 3 cut(s) 571, 577, 727
GluI GCNGC 7 cut(s) 469, 472, 568, 686, 729, 1137, 1247
GsuI CTGGAG 3 cut(s) 110, 309, 924
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 7 cut(s) 306, 419, 1100, 1211, 1267, 1332, 1340
HapII CCGG 2 cut(s) 991, 1074
HgaI GACGC 2 cut(s) 697, 859
HhaI GCGC 3 cut(s) 572, 578, 728
Hin1I GRCGYC 1 cut(s) 870
Hin1II CATG 9 cut(s) 105, 180, 277, 388, 406, 562, 1309, 1338, 1375
Hin6I GCGC 3 cut(s) 570, 576, 726
HinP1I GCGC 3 cut(s) 570, 576, 726
HindIII AAGCTT 1 cut(s) 1112
HinfI GANTC 4 cut(s) 245, 365, 851, 1319
HpaII CCGG 2 cut(s) 991, 1074
HphI GGTGA 4 cut(s) 112, 865, 1099, 1244
Hpy166II GTNNAC 5 cut(s) 169, 382, 463, 616, 1014
Hpy188I TCNGA 5 cut(s) 227, 609, 716, 1143, 1362
Hpy188III TCNNGA 4 cut(s) 787, 893, 991, 1039
Hpy8I GTNNAC 5 cut(s) 169, 382, 463, 616, 1014
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 5 cut(s) 220, 329, 492, 498, 1321
HpyCH4III ACNGT 3 cut(s) 548, 783, 1045
HpyCH4IV ACGT 2 cut(s) 534, 1026
HpyCH4V TGCA 8 cut(s) 64, 201, 468, 567, 674, 1153, 1275, 1296
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 477, 564, 573
HpyF3I CTNAG 3 cut(s) 641, 690, 794
HpySE526I ACGT 2 cut(s) 534, 1026
Hsp92I GRCGYC 1 cut(s) 870
Hsp92II CATG 9 cut(s) 105, 180, 277, 388, 406, 562, 1309, 1338, 1375
HspAI GCGC 3 cut(s) 570, 576, 726
Kpn2I TCCGGA 1 cut(s) 990
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 5 cut(s) 108, 409, 696, 1047, 1375
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 3 cut(s) 27, 200, 723
Lsp1109I GCAGC 5 cut(s) 480, 579, 697, 1123, 1258
LweI GCATC 2 cut(s) 513, 1356
MaeI CTAG 2 cut(s) 893, 1278
MaeII ACGT 2 cut(s) 534, 1026
MaeIII GTNAC 4 cut(s) 34, 299, 871, 909
MalI GATC 5 cut(s) 110, 411, 698, 1049, 1377
MboI GATC 5 cut(s) 108, 409, 696, 1047, 1375
MfeI CAATTG 1 cut(s) 703
MhlI GDGCHC 3 cut(s) 288, 393, 1334
MlsI TGGCCA 2 cut(s) 419, 1100
MluNI TGGCCA 2 cut(s) 419, 1100
MlyI GAGTC 2 cut(s) 845, 1328
MmeI TCCRAC 1 cut(s) 899
Mox20I TGGCCA 2 cut(s) 419, 1100
Mph1103I ATGCAT 1 cut(s) 1298
MroI TCCGGA 1 cut(s) 990
MroXI GAANNNNTTC 1 cut(s) 966
MscI TGGCCA 2 cut(s) 419, 1100
MseI TTAA 1 cut(s) 182
Msp20I TGGCCA 2 cut(s) 419, 1100
MspA1I CMGCKG 1 cut(s) 471
MspI CCGG 2 cut(s) 991, 1074
MspR9I CCNGG 1 cut(s) 1075
MunI CAATTG 1 cut(s) 703
MvnI CGCG 2 cut(s) 728, 843
MwoI GCNNNNNNNGC 4 cut(s) 275, 477, 564, 573
NciI CCSGG 1 cut(s) 1075
NcoI CCATGG 3 cut(s) 1305, 1334, 1371
NdeII GATC 5 cut(s) 108, 409, 696, 1047, 1375
NlaIII CATG 9 cut(s) 105, 180, 277, 388, 406, 562, 1309, 1338, 1375
NlaIV GGNNCC 2 cut(s) 1166, 1332
NmeAIII GCCGAG 1 cut(s) 915
NmuCI GTSAC 2 cut(s) 299, 871
NsiI ATGCAT 1 cut(s) 1298
NspV TTCGAA 2 cut(s) 243, 770
PciSI GCTCTTC 1 cut(s) 946
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 2 cut(s) 245, 365
PfoI TCCNGGA 1 cut(s) 1073
PkrI GCNGC 7 cut(s) 470, 473, 569, 687, 730, 1138, 1248
PleI GAGTC 2 cut(s) 845, 1327
PpsI GAGTC 2 cut(s) 845, 1327
Ppu21I YACGTR 1 cut(s) 1027
PspN4I GGNNCC 2 cut(s) 1166, 1332
PspOMI GGGCCC 1 cut(s) 1330
PspPI GGNCC 5 cut(s) 1265, 1302, 1330, 1331, 1338
PstI CTGCAG 1 cut(s) 470
RsaI GTAC 2 cut(s) 518, 1001
RsaNI GTAC 2 cut(s) 517, 1000
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 1 cut(s) 182
SatI GCNGC 7 cut(s) 469, 472, 568, 686, 729, 1137, 1247
Sau3AI GATC 5 cut(s) 108, 409, 696, 1047, 1375
Sau96I GGNCC 5 cut(s) 1265, 1302, 1330, 1331, 1338
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 845, 1328
ScrFI CCNGG 1 cut(s) 1075
SduI GDGCHC 3 cut(s) 288, 393, 1334
SfaNI GCATC 2 cut(s) 513, 1356
SfcI CTRYAG 3 cut(s) 466, 544, 1014
SfuI TTCGAA 2 cut(s) 243, 770
SinI GGWCC 1 cut(s) 1302
SpeI ACTAGT 1 cut(s) 1277
SsiI CCGC 3 cut(s) 437, 471, 728
SspMI CTAG 2 cut(s) 893, 1278
StyD4I CCNGG 1 cut(s) 1073
StyI CCWWGG 3 cut(s) 1305, 1334, 1371
TaaI ACNGT 3 cut(s) 548, 783, 1045
TaiI ACGT 2 cut(s) 537, 1029
TatI WGTACW 1 cut(s) 516
TauI GCSGC 2 cut(s) 474, 731
TfiI GAWTC 2 cut(s) 245, 365
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 2 cut(s) 48, 1155
TseFI GTSAC 2 cut(s) 299, 871
TseI GCWGC 5 cut(s) 468, 567, 685, 1136, 1246
Tsp45I GTSAC 2 cut(s) 299, 871
TspDTI ATGAA 5 cut(s) 79, 165, 262, 779, 1206
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 2 cut(s) 48, 1155
VpaK11BI GGWCC 1 cut(s) 1302
XapI RAATTY 5 cut(s) 218, 622, 766, 965, 1033
XbaI TCTAGA 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 2 cut(s) 893, 1278
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.