Rroxscaffold_4G00282560

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012744
Physical Location & Seq
Reverse (-)
4641703 .. 4643537
1835 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00282560.1

Sequence Viewer

Length: 537 bp
ATGGCAGCTCTAGTTCCTGAGCAATGCCTCAACTCCACCACCCCTTCCACTTTTTTATCAAAGCAGATTCTTTCAACTCTCTCCCAAAGTCTAGGACTTTTGGTGCTCATACTTGTAACATCCCTTGAAGCCTTGTTTCTGCTCCAAAAAAGACCACCCATTTTTCACTTCATTAACCTCTCTTGCTTTACCATCGTCTTCATCCTCAAGCCTTACCTCTCCAAAAAGACTCATAAAGTCTATTTGGTAGACTTCTCATGTCTAAAACCACCAAGCTCCTGCAAAGTCCCCTTCTCCAAATTCCTCAAGAACTCCTCCATGATTGAAGGTTTCGACAGTGAAAGCATATCTTTCATGGCCAACATCCTCACTTCTTCGGGCCAAAGCCAAGAGACCTATCTCCCTCCAGCTTTACACCACATTCCTCCCAAAACCCACCACCATGAATACATCAAAGAAGTTCACATGGTCCTTTTCCCCATCATGGATGACCTTTTGGCCAAAACCAAACTCTCCCTCAAGACATTGACATCCTAA

Protein Analysis

178

Amino Acids

19.98

Weight (kDa)

9.05

Isoelectric Point (pI)

60.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 72 - 173 9.2e-23 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 249
AcoI YGGCCR 2 cut(s) 357, 498
AcsI RAATTY 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 3 cut(s) 75, 128, 326
AluBI AGCT 3 cut(s) 8, 276, 410
AluI AGCT 3 cut(s) 8, 276, 410
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 1 cut(s) 386
AoxI GGCC 3 cut(s) 357, 379, 498
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 379, 469
AvaII GGWCC 1 cut(s) 469
BalI TGGCCA 2 cut(s) 359, 500
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 200, 488
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 2 cut(s) 11, 92
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 2 cut(s) 379, 469
BpiI GAAGAC 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 3 cut(s) 191, 290, 503
BsaI GGTCTC 1 cut(s) 386
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse3DI GCAATG 1 cut(s) 29
BseGI GGATG 5 cut(s) 119, 201, 363, 493, 530
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 3 cut(s) 359, 381, 500
BsiHKAI GWGCWC 1 cut(s) 108
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 3 cut(s) 359, 381, 500
Bso31I GGTCTC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 108
BspANI GGCC 3 cut(s) 359, 381, 500
BspCNI CTCAG 1 cut(s) 10
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 338
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 5 cut(s) 119, 201, 363, 493, 530
BstMAI GTCTC 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 3 cut(s) 359, 381, 500
BtsCI GGATG 5 cut(s) 119, 201, 363, 493, 530
BtsIMutI CAGTG 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 379, 469
CviAII CATG 6 cut(s) 258, 319, 355, 443, 466, 484
CviJI RGCY 9 cut(s) 8, 131, 211, 276, 359, 381, 387, 410, 500
CviKI_1 RGCY 9 cut(s) 8, 131, 211, 276, 359, 381, 387, 410, 500
DdeI CTNAG 1 cut(s) 18
EaeI YGGCCR 2 cut(s) 357, 498
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 469
FaeI CATG 6 cut(s) 261, 322, 358, 446, 469, 487
FaiI YATR 9 cut(s) 110, 234, 259, 320, 347, 356, 444, 467, 485
FalI AAGNNNNNCTT 2 cut(s) 334, 366
FaqI GGGAC 1 cut(s) 272
FatI CATG 6 cut(s) 257, 318, 354, 442, 465, 483
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 5 cut(s) 106, 188, 350, 500, 517
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 11, 92
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 3 cut(s) 359, 381, 500
Hin1II CATG 6 cut(s) 261, 322, 358, 446, 469, 487
HinfI GANTC 2 cut(s) 67, 229
Hpy166II GTNNAC 2 cut(s) 250, 463
Hpy188III TCNNGA 3 cut(s) 17, 307, 520
Hpy8I GTNNAC 2 cut(s) 250, 463
HpyAV CCTTC 3 cut(s) 54, 301, 320
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4V TGCA 1 cut(s) 282
HpyF3I CTNAG 1 cut(s) 18
Hsp92II CATG 6 cut(s) 261, 322, 358, 446, 469, 487
LmnI GCTCC 2 cut(s) 147, 281
LpnPI CCDG 3 cut(s) 30, 292, 420
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 2 cut(s) 11, 92
MaeIII GTNAC 1 cut(s) 115
MboII GAAGA 2 cut(s) 190, 366
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 2 cut(s) 359, 500
MluCI AATT 1 cut(s) 299
MluNI TGGCCA 2 cut(s) 359, 500
MlyI GAGTC 1 cut(s) 223
Mox20I TGGCCA 2 cut(s) 359, 500
MscI TGGCCA 2 cut(s) 359, 500
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 2 cut(s) 359, 500
NlaIII CATG 6 cut(s) 261, 322, 358, 446, 469, 487
PfeI GAWTC 1 cut(s) 67
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PspPI GGNCC 2 cut(s) 379, 469
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 6
Sau96I GGNCC 2 cut(s) 379, 469
SchI GAGTC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 108
SetI ASST 8 cut(s) 10, 180, 219, 278, 331, 398, 412, 495
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 3 cut(s) 206, 305, 518
SmoI CTYRAG 3 cut(s) 206, 305, 518
Sse9I AATT 1 cut(s) 299
SspMI CTAG 2 cut(s) 11, 92
TaaI ACNGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 299
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 343
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 4 cut(s) 160, 190, 343, 459
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 2 cut(s) 11, 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.