FvH4_7g28500

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012744
Physical Location & Seq
Forward (+)
21124148 .. 21126907
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28500.t1

Sequence Viewer

Length: 1425 bp
ATGGCACCTCTAGTTTCAAAGCAGATAGTTTCAACTCTCTCCCAATGTCTAGGACTTCTGGTGCTCATACTTGTAACATCCCTTGAAGCCTTGTTTCTCCTCCAAAAAAGTCCACCCATTTTCCACTTGATTAACCTCTCTTGCTTCACCATCTTCTTCATCCTCAAGCCTTACCTCTCCAAAAAGACTCACAAAATCTACTTGGTAGACTTCTCATGTCTAAAACCCCCAAGCCACTGCAAAGTCCCCTTCTCCAAATTCCTCAAGAACTCCTCCATGATTGAAGGTTTCGACAGCGAAAGCGTAGCTTTCATGGCCAACATCCTCGCTTCTTCCGGACAGAGCCAAGAGACTTATCTCCCTCCAGCCTTGCACCACATTCCTCCCAAAACCCACCACCATGAATACATCAAAGAAGTCCATATGGTACTTTTCCCCATCATGGATGACCTTTTGGCCAAAACCAAACTATCCCCTCAAGACATTGACATCCTAATAGTAAACTGCAGCGGTTTCAATCCCACTCCTTCTCTAACCTCCATTGTCATCAACCACTACTCAATGAAATCTGACATCAAGAGCTTCAACCTCTCCGGAATGGGCTGCAGCGCAGGCGCTTTGGGCATTGATATGGCCCAAAACCTCCTCCAGATTCACAAGAACTCATACGCCGTCGTTTTGAGCACAGAGATTCTATCAACAGGCTGGTATGCAGGTCATGAAAAGCCCAAGTTGATCCTCAACTGCCTCTTCAGAATGGGCAGCGCCGCCATTTTGCTCACAAACAAAAACCAAACATCCAAACATCCAAAGTACGAGCTCTTCAAAACTCTCAGAACGAATAAATCCTACGACGACAGAGCTTACCTCTCCACCATCCGAGAAGAAGACTCGGAAGGAAAACTCGGGGTCACCATCAAACGTGATCTCCTCCAAGTCGCCGGCGAGACGCTCCGAGCAAACGTCACCGTTTTGGGGTCGTCAATCCTTCCGTTGTCCGAGAAGTTCTGGCACGGCGTTTCCATAATCAGGAAGAGATTCTTGAACAAGTCTGCGGAGATTTACGTGCCGAATTTCAAGAGTGTGATAGAGCATTTCTGTCTGCCGGCGTCAGGGAGGCCGGTGATAAGGGAGATCGGAAAAGGGTTGAAGCTTGGAGATAGGGAAATGGAGGCTGCCGTGATGACGTTGCACCGGTTTGGGAACCAGTCGTCTTCTGCACTGTGGTATGAATTGGCGTACTTGGAGGGAAAAGAGAGAGTGAAGAAAGGTGACAAGGTTTGGCAGCTTGGGATGGGCACTGGGCCGAAGTGCACTAGTTTGGTTTGGAAGTGTATAAGGCCCATTGAGGGTGAGTCCAAGAGAGGCCCGTGGGCCGATTGCATCCATCGATATCCAATTGGAGCCCACGAAGAAGCTAATTAG

Protein Analysis

475

Amino Acids

52.99

Weight (kDa)

9.15

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 59 - 346 6.3e-109 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 363 - 444 3.3e-07 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15090 AT2G46720 AT3G10280 AT4G34250 AT4G34520
fragaria_vesca FvH4_7g28500
malus_domestica MD01G1188700.v1.1
prunus_persica Prupe.2G285400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0375881
rosa_laevigata RLG00000026647
rosa_multiflora Rmu_sc0001943.1_g000009
rosa_roxburghii Rroxscaffold_4G00282560
rosa_rugosa Rorug01G0392100
rosa_samantha Rh1AG404000 Rh1BG367400 Rh1CG380200 Rh1DG397400
rosa_wichuraiana Rw0G000970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 207
AccIII TCCGGA 2 cut(s) 335, 593
AciI CCGC 3 cut(s) 510, 768, 1055
AclWI GGATC 1 cut(s) 730
AcoI YGGCCR 2 cut(s) 315, 456
AcsI RAATTY 2 cut(s) 257, 1072
AcuI CTGAAG 1 cut(s) 736
AcyI GRCGYC 1 cut(s) 1109
AfaI GTAC 3 cut(s) 429, 815, 1241
AfiI CCNNNNNNNGG 5 cut(s) 442, 975, 1029, 1112, 1349
AgeI ACCGGT 1 cut(s) 1194
AhlI ACTAGT 1 cut(s) 1316
AluBI AGCT 7 cut(s) 308, 582, 820, 863, 1153, 1288, 1418
AluI AGCT 7 cut(s) 308, 582, 820, 863, 1153, 1288, 1418
Alw21I GWGCWC 4 cut(s) 66, 686, 822, 1316
Alw26I GTCTC 2 cut(s) 344, 941
Alw44I GTGCAC 1 cut(s) 1312
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 905
Aor13HI TCCGGA 2 cut(s) 335, 593
AoxI GGCC 8 cut(s) 315, 456, 633, 1118, 1304, 1340, 1366, 1374
ApaLI GTGCAC 1 cut(s) 1312
ApeKI GCWGC 6 cut(s) 507, 603, 606, 762, 1175, 1285
ApoI RAATTY 2 cut(s) 257, 1072
AsiGI ACCGGT 1 cut(s) 1194
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 3 cut(s) 611, 617, 767
AspS9I GGNCC 5 cut(s) 634, 1304, 1341, 1367, 1374
AsuHPI GGTGA 6 cut(s) 139, 904, 958, 1135, 1283, 1364
AvaI CYCGRG 1 cut(s) 905
BaeGI GKGCMC 2 cut(s) 1301, 1316
BalI TGGCCA 2 cut(s) 317, 458
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 822, 1408
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BbsI GAAGAC 2 cut(s) 894, 1206
Bbv12I GWGCWC 4 cut(s) 66, 686, 822, 1316
BbvI GCAGC 6 cut(s) 519, 590, 618, 774, 1162, 1297
BccI CCATC 6 cut(s) 158, 446, 884, 923, 1288, 1395
BceAI ACGGC 3 cut(s) 656, 1030, 1163
BcoDI GTCTC 2 cut(s) 344, 941
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 3 cut(s) 11, 50, 1317
BfmI CTRYAG 2 cut(s) 505, 604
BfoI RGCGCY 2 cut(s) 618, 768
BfuAI ACCTGC 1 cut(s) 704
BisI GCNGC 7 cut(s) 508, 604, 607, 763, 768, 1176, 1286
BlsI GCNGC 7 cut(s) 509, 605, 608, 764, 769, 1177, 1287
BmeT110I CYCGRG 1 cut(s) 905
BmgT120I GGNCC 5 cut(s) 634, 1304, 1341, 1367, 1374
BmiI GGNNCC 3 cut(s) 6, 1205, 1405
BmrI ACTGGG 1 cut(s) 1311
BmsI GCATC 1 cut(s) 1392
BmuI ACTGGG 1 cut(s) 1311
BpiI GAAGAC 2 cut(s) 894, 1206
BplI GAGNNNNNCTC 2 cut(s) 852, 884
BpmI CTGGAG 2 cut(s) 348, 632
BpuEI CTTGAG 3 cut(s) 149, 248, 462
Bsa29I ATCGAT 1 cut(s) 1390
BsaAI YACGTR 1 cut(s) 1066
BsaHI GRCGYC 1 cut(s) 1109
BsaJI CCNNGG 1 cut(s) 1370
BsaWI WCCGGW 3 cut(s) 335, 593, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 5 cut(s) 442, 975, 1029, 1112, 1349
Bse118I RCCGGY 4 cut(s) 941, 1105, 1120, 1194
Bse1I ACTGG 2 cut(s) 1207, 1306
BseAI TCCGGA 2 cut(s) 335, 593
BseCI ATCGAT 1 cut(s) 1390
BseDI CCNNGG 1 cut(s) 1370
BseLI CCNNNNNNNGG 5 cut(s) 442, 975, 1029, 1112, 1349
BseMII CTCAG 1 cut(s) 847
BseNI ACTGG 2 cut(s) 1207, 1306
BseRI GAGGAG 4 cut(s) 89, 262, 635, 920
BseSI GKGCMC 2 cut(s) 1301, 1316
BseXI GCAGC 6 cut(s) 519, 590, 618, 774, 1162, 1297
BsgI GTGCAG 1 cut(s) 1203
BshFI GGCC 8 cut(s) 317, 458, 635, 1120, 1306, 1342, 1368, 1376
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 1194
BshVI ATCGAT 1 cut(s) 1390
BsiHKAI GWGCWC 4 cut(s) 66, 686, 822, 1316
BsiHKCI CYCGRG 1 cut(s) 905
BsiSI CCGG 6 cut(s) 336, 594, 942, 1106, 1121, 1195
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 5 cut(s) 442, 975, 1029, 1112, 1349
BsmAI GTCTC 2 cut(s) 344, 941
BsmBI CGTCTC 1 cut(s) 941
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 8 cut(s) 317, 458, 635, 1120, 1306, 1342, 1368, 1376
BsoBI CYCGRG 1 cut(s) 905
Bsp1286I GDGCHC 6 cut(s) 66, 686, 822, 1301, 1316, 1408
Bsp13I TCCGGA 2 cut(s) 335, 593
Bsp143I GATC 3 cut(s) 735, 925, 1134
BspACI CCGC 3 cut(s) 510, 768, 1055
BspANI GGCC 8 cut(s) 317, 458, 635, 1120, 1306, 1342, 1368, 1376
BspCNI CTCAG 1 cut(s) 846
BspDI ATCGAT 1 cut(s) 1390
BspEI TCCGGA 2 cut(s) 335, 593
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 3 cut(s) 6, 1205, 1405
BspMAI CTGCAG 2 cut(s) 509, 608
BspMI ACCTGC 1 cut(s) 704
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 4 cut(s) 941, 1105, 1120, 1194
BsrI ACTGG 2 cut(s) 1207, 1306
BssAI RCCGGY 4 cut(s) 941, 1105, 1120, 1194
BssECI CCNNGG 1 cut(s) 1370
BssMI GATC 3 cut(s) 735, 925, 1134
BssNI GRCGYC 1 cut(s) 1109
Bst4CI ACNGT 2 cut(s) 970, 1224
Bst6I CTCTTC 3 cut(s) 755, 827, 1028
BstACI GRCGYC 1 cut(s) 1109
BstBAI YACGTR 1 cut(s) 1066
BstC8I GCNNGC 3 cut(s) 613, 943, 1107
BstDEI CTNAG 1 cut(s) 833
BstDSI CCRYGG 1 cut(s) 1370
BstEII GGTNACC 1 cut(s) 910
BstH2I RGCGCY 2 cut(s) 618, 768
BstHHI GCGC 3 cut(s) 611, 617, 767
BstKTI GATC 3 cut(s) 738, 928, 1137
BstMAI GTCTC 2 cut(s) 344, 941
BstMBI GATC 3 cut(s) 735, 925, 1134
BstMWI GCNNNNNNNGC 3 cut(s) 314, 612, 621
BstPI GGTNACC 1 cut(s) 910
BstSFI CTRYAG 2 cut(s) 505, 604
BstSLI GKGCMC 2 cut(s) 1301, 1316
BstV1I GCAGC 6 cut(s) 519, 590, 618, 774, 1162, 1297
BstV2I GAAGAC 2 cut(s) 894, 1206
Bsu15I ATCGAT 1 cut(s) 1390
BsuRI GGCC 8 cut(s) 317, 458, 635, 1120, 1306, 1342, 1368, 1376
BsuTUI ATCGAT 1 cut(s) 1390
BtgI CCRYGG 1 cut(s) 1370
BtsI GCAGTG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 235, 1220, 1299
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 3 cut(s) 613, 943, 1107
CciI TCATGA 1 cut(s) 718
CfoI GCGC 3 cut(s) 611, 617, 767
Cfr10I RCCGGY 4 cut(s) 941, 1105, 1120, 1194
Cfr13I GGNCC 5 cut(s) 634, 1304, 1341, 1367, 1374
ClaI ATCGAT 1 cut(s) 1390
CseI GACGC 2 cut(s) 958, 1098
Csp6I GTAC 3 cut(s) 428, 814, 1240
CspAI ACCGGT 1 cut(s) 1194
CviAII CATG 6 cut(s) 216, 277, 313, 401, 442, 719
CviQI GTAC 3 cut(s) 428, 814, 1240
DdeI CTNAG 1 cut(s) 833
DpnI GATC 3 cut(s) 737, 927, 1136
DpnII GATC 3 cut(s) 735, 925, 1134
EaeI YGGCCR 2 cut(s) 315, 456
Eam1104I CTCTTC 3 cut(s) 755, 827, 1028
EarI CTCTTC 3 cut(s) 755, 827, 1028
Ecl136II GAGCTC 1 cut(s) 820
Eco24I GRGCYC 2 cut(s) 822, 1408
Eco32I GATATC 1 cut(s) 1394
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 736
Eco88I CYCGRG 1 cut(s) 905
Eco91I GGTNACC 1 cut(s) 910
EcoICRI GAGCTC 1 cut(s) 820
EcoO65I GGTNACC 1 cut(s) 910
EcoRV GATATC 1 cut(s) 1394
EcoT38I GRGCYC 2 cut(s) 822, 1408
Esp3I CGTCTC 1 cut(s) 941
FaeI CATG 6 cut(s) 219, 280, 316, 404, 445, 722
FalI AAGNNNNNCTT 4 cut(s) 292, 324, 1025, 1057
FaqI GGGAC 1 cut(s) 230
FatI CATG 6 cut(s) 215, 276, 312, 400, 441, 718
FauNDI CATATG 1 cut(s) 423
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 7 cut(s) 508, 604, 607, 763, 768, 1176, 1286
FriOI GRGCYC 2 cut(s) 822, 1408
Fsp4HI GCNGC 7 cut(s) 508, 604, 607, 763, 768, 1176, 1286
FspBI CTAG 3 cut(s) 11, 50, 1317
GlaI GCGC 3 cut(s) 610, 616, 766
GluI GCNGC 7 cut(s) 508, 604, 607, 763, 768, 1176, 1286
GsuI CTGGAG 2 cut(s) 348, 632
HaeII RGCGCY 2 cut(s) 618, 768
HaeIII GGCC 8 cut(s) 317, 458, 635, 1120, 1306, 1342, 1368, 1376
HapII CCGG 6 cut(s) 336, 594, 942, 1106, 1121, 1195
HgaI GACGC 2 cut(s) 958, 1098
HhaI GCGC 3 cut(s) 611, 617, 767
Hin1I GRCGYC 1 cut(s) 1109
Hin1II CATG 6 cut(s) 219, 280, 316, 404, 445, 722
Hin6I GCGC 3 cut(s) 609, 615, 765
HinP1I GCGC 3 cut(s) 609, 615, 765
HindIII AAGCTT 1 cut(s) 1151
HinfI GANTC 6 cut(s) 187, 652, 691, 890, 1038, 1355
HpaII CCGG 6 cut(s) 336, 594, 942, 1106, 1121, 1195
HphI GGTGA 6 cut(s) 139, 904, 958, 1135, 1283, 1364
Hpy166II GTNNAC 4 cut(s) 113, 208, 502, 1314
Hpy188I TCNGA 8 cut(s) 571, 755, 836, 881, 895, 956, 1000, 1139
Hpy8I GTNNAC 4 cut(s) 113, 208, 502, 1314
Hpy99I CGWCG 2 cut(s) 677, 857
HpyAV CCTTC 5 cut(s) 259, 278, 537, 890, 998
HpyCH4III ACNGT 2 cut(s) 970, 1224
HpyCH4IV ACGT 4 cut(s) 922, 963, 1065, 1187
HpyCH4V TGCA 9 cut(s) 240, 373, 507, 606, 713, 1192, 1220, 1314, 1383
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 612, 621
HpyF3I CTNAG 1 cut(s) 833
HpySE526I ACGT 4 cut(s) 922, 963, 1065, 1187
Hsp92I GRCGYC 1 cut(s) 1109
Hsp92II CATG 6 cut(s) 219, 280, 316, 404, 445, 722
HspAI GCGC 3 cut(s) 609, 615, 765
Kpn2I TCCGGA 2 cut(s) 335, 593
KroI GCCGGC 2 cut(s) 941, 1105
KroNI GCCGGC 2 cut(s) 943, 1107
Kzo9I GATC 3 cut(s) 735, 925, 1134
LguI GCTCTTC 1 cut(s) 827
LmnI GCTCC 2 cut(s) 957, 1403
Lsp1109I GCAGC 6 cut(s) 519, 590, 618, 774, 1162, 1297
LweI GCATC 1 cut(s) 1392
MaeI CTAG 3 cut(s) 11, 50, 1317
MaeII ACGT 4 cut(s) 922, 963, 1065, 1187
MaeIII GTNAC 4 cut(s) 73, 910, 964, 1271
MalI GATC 3 cut(s) 737, 927, 1136
MboI GATC 3 cut(s) 735, 925, 1134
MfeI CAATTG 1 cut(s) 1398
MhlI GDGCHC 6 cut(s) 66, 686, 822, 1301, 1316, 1408
MlsI TGGCCA 2 cut(s) 317, 458
MluCI AATT 5 cut(s) 257, 1072, 1232, 1398, 1420
MluNI TGGCCA 2 cut(s) 317, 458
MlyI GAGTC 3 cut(s) 181, 884, 1364
Mox20I TGGCCA 2 cut(s) 317, 458
MreI CGCCGGCG 1 cut(s) 941
MroI TCCGGA 2 cut(s) 335, 593
MroNI GCCGGC 2 cut(s) 941, 1105
MroXI GAANNNNTTC 1 cut(s) 1037
MscI TGGCCA 2 cut(s) 317, 458
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 2 cut(s) 399, 629
Msp20I TGGCCA 2 cut(s) 317, 458
MspA1I CMGCKG 1 cut(s) 510
MspI CCGG 6 cut(s) 336, 594, 942, 1106, 1121, 1195
MunI CAATTG 1 cut(s) 1398
MwoI GCNNNNNNNGC 3 cut(s) 314, 612, 621
NaeI GCCGGC 2 cut(s) 943, 1107
NdeI CATATG 1 cut(s) 423
NdeII GATC 3 cut(s) 735, 925, 1134
NgoMIV GCCGGC 2 cut(s) 941, 1105
NlaIII CATG 6 cut(s) 219, 280, 316, 404, 445, 722
NlaIV GGNNCC 3 cut(s) 6, 1205, 1405
NmuCI GTSAC 3 cut(s) 910, 964, 1271
PagI TCATGA 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 827
PdiI GCCGGC 2 cut(s) 943, 1107
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 3 cut(s) 652, 691, 1038
PinAI ACCGGT 1 cut(s) 1194
PkrI GCNGC 7 cut(s) 509, 605, 608, 764, 769, 1177, 1287
PleI GAGTC 3 cut(s) 181, 884, 1363
PpsI GAGTC 3 cut(s) 181, 884, 1363
Ppu21I YACGTR 1 cut(s) 1066
Psp124BI GAGCTC 1 cut(s) 822
PspEI GGTNACC 1 cut(s) 910
PspN4I GGNNCC 3 cut(s) 6, 1205, 1405
PspPI GGNCC 5 cut(s) 634, 1304, 1341, 1367, 1374
PstI CTGCAG 2 cut(s) 509, 608
RsaI GTAC 3 cut(s) 429, 815, 1241
RsaNI GTAC 3 cut(s) 428, 814, 1240
RseI CAYNNNNRTG 2 cut(s) 399, 629
SacI GAGCTC 1 cut(s) 822
SapI GCTCTTC 1 cut(s) 827
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 7 cut(s) 508, 604, 607, 763, 768, 1176, 1286
Sau3AI GATC 3 cut(s) 735, 925, 1134
Sau96I GGNCC 5 cut(s) 634, 1304, 1341, 1367, 1374
SchI GAGTC 3 cut(s) 181, 884, 1364
SduI GDGCHC 6 cut(s) 66, 686, 822, 1301, 1316, 1408
SfaNI GCATC 1 cut(s) 1392
SfcI CTRYAG 2 cut(s) 505, 604
SgrAI CRCCGGYG 1 cut(s) 941
SmiMI CAYNNNNRTG 2 cut(s) 399, 629
SmlI CTYRAG 3 cut(s) 164, 263, 477
SmoI CTYRAG 3 cut(s) 164, 263, 477
SpeI ACTAGT 1 cut(s) 1316
Sse9I AATT 5 cut(s) 257, 1072, 1232, 1398, 1420
SsiI CCGC 3 cut(s) 510, 768, 1055
SspMI CTAG 3 cut(s) 11, 50, 1317
SstI GAGCTC 1 cut(s) 822
TaaI ACNGT 2 cut(s) 970, 1224
TaiI ACGT 4 cut(s) 925, 966, 1068, 1190
TaqI TCGA 2 cut(s) 291, 1390
TasI AATT 5 cut(s) 257, 1072, 1232, 1398, 1420
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 3 cut(s) 652, 691, 1038
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 3 cut(s) 242, 1227, 1306
TseFI GTSAC 3 cut(s) 910, 964, 1271
TseI GCWGC 6 cut(s) 507, 603, 606, 762, 1175, 1285
Tsp45I GTSAC 3 cut(s) 910, 964, 1271
TspDTI ATGAA 6 cut(s) 148, 301, 417, 578, 735, 1245
TspGWI ACGGA 1 cut(s) 981
TspRI CASTG 3 cut(s) 242, 1227, 1306
VneI GTGCAC 1 cut(s) 1312
XapI RAATTY 2 cut(s) 257, 1072
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 3 cut(s) 11, 50, 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.