AT3G12810
MYB Family

Photoperiod-independent early flowering

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014066
Physical Location & Seq
Forward (+)
4065042 .. 4074194
9153 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12810.1

Sequence Viewer

Length: 6168 bp
ATGGCGTCTAAAGGTGGTAAATCTAAACCTGATATAGTAATGGCGTCCAAGAGTGGGAAATCTAAACCTGATAATGAATCCAGAGCTAAGCGTCAGAAGACGCTCGAAGCTCCTAAAGAACCACGTCGTCCTAAAACTCACTGGGACCATGTATTGGAGGAGATGGCTTGGCTGTCAAAGGACTTTGAGTCTGAGAGGAAATGGAAGCTGGCGCAGGCGAAGAAGGTTGCTTTGAGAGCCAGCAAAGGAATGCTAGATCAGGCATCTAGGGAAGAAAGGAAGCTAAAGGAAGAAGAACAGCGACTTCGGAAAGTAGCCCTCAATATCTCGAAAGACATGAAAAAGTTTTGGATGAAAGTTGAGAAGCTGGTGCTTTACAAGCATCAGTTGGTACGCAATGAGAAGAAGAAGAAGGCTATGGACAAGCAACTTGAATTTCTGTTAGGCCAAACTGAGAGGTACTCAACCATGTTGGCAGAAAATTTAGTGGAGCCTTATAAACAGGGCCAAAATACTCCTTCAAAACCTCTACTAACTATCGAATCAAAAAGTGATGAGGAGAGGGCAGAACAGATACCTCCAGAGATAAATTCTTCTGCAGGTCTTGAATCAGGAAGTCCTGAACTCGATGAGGATTATGATCTAAAATCTGAGGATGAGACCGAAGATGATGAAGATACTATTGAGGAGGATGAAAAACACTTCACCAAACGTGAAAGACAGGAAGAGTTGGAAGCTTTGCAAAATGAAGTGGATCTACCAGTTGAGGAGCTGCTCAGACGTTATACCTCTGGAAGAGTTAGCCGAGAAACCAGCCCAGTAAAAGATGAAAATGAGGACAATCTGACCTCAGTTAGCCGAGTAACCAGCCCAGTAAAAGATGAAAATCAGGACAATCTGGCCTCAGTAGGTCAAGATCATGGAGAGGACAAGAACAATCTTGCAGCTTCTGAAGAAACTGAAGGGAACCCAAGTGTTCGTCGTTCTAATGATAGCTATGGTCATTTGGCAATATCCGAGACCCATTCACATGACCTTGAGCCAGGCATGACGACTGCATCTGTGAAGTCCAGAAAGGAAGATCATACCTATGATTTTAACGATGAACAGGAAGATGTTGACTTTGTTCTTGCCAATGGTGAGGAAAAGGATGATGAGGCGACATTGGCTGTCGAAGAGGAACTGGCGAAAGCAGATAATGAAGATCATGTCGAAGAGATTGCCCTGCTGCAAAAAGAGAGTGAAATGCCTATTGAAGTACTACTTGCAAGGTATAAGGAGGATTTCGGTGGCAAGGATATATCTGAGGATGAATCTGAATCTTCATTTGCTGTATCTGAAGACTCTATAGTAGACTCTGACGAAAATAGGCAGCAAGCTGATTTAGATGATGACAACGTTGATTTAACAGAATGTAAGCTAGATCCAGAGCCTTGCTCAGAAAATGTTGAAGGCACATTCCATGAAGTAGCAGAAGATAATGATAAAGATAGTAGTGATAAAATTGCTGATGCAGCAGCAGCAGCAAGATCTGCCCAGCCAACTGGATTTACATACTCCACAACAAAAGTTCGAACGAAACTGCCGTTTCTACTTAAGCATTCTCTGCGTGAGTACCAACACATTGGTTTGGACTGGCTTGTCACGATGTATGAGAAAAAGTTGAACGGTATTCTGGCTGATGAAATGGGTCTTGGAAAGACAATTATGACCATTGCTCTTCTAGCTCACCTTGCATGTGATAAGGGAATATGGGGTCCCCATCTGATTGTGGTTCCTACAAGTGTGATGCTTAATTGGGAGACTGAGTTTCTTAAATGGTGTCCTGCATTTAAAATTCTCACCTACTTTGGTAGTGCAAAGGAGCGGAAACTCAAGAGACAAGGATGGATGAAGCTTAACTCATTTCATGTATGCATAACAACCTATAGGCTAGTTATTCAGGACAGTAAAATGTTCAAGCGCAAGAAGTGGAAATACTTGATTCTTGACGAAGCCCATTTAATTAAGAATTGGAAGTCTCAGAGATGGCAGACACTATTGAACTTCAATTCTAAACGCAGAATTTTGTTGACTGGTACACCCCTGCAGAATGATCTTATGGAACTGTGGTCACTTATGCACTTTCTGATGCCACATGTCTTTCAGTCTCACCAGGAATTCAAAGACTGGTTCTGTAACCCAATAGCAGGGATGGTCGAGGGACAAGAAAAAATCAACAAAGAAGTTATTGATCGCTTACATAATGTCCTCCGTCCTTTCCTTCTACGGCGCCTAAAAAGGGATGTGGAGAAGCAACTTCCTTCGAAACATGAGCATGTTATTTTCTGTAGACTGTCTAAGAGGCAGCGCAACTTGTATGAGGACTTCATAGCAAGCACAGAAACACAGGCTACGCTTACCAGTGGAAGCTTTTTTGGGATGATAAGTATCATAATGCAGTTGCGGAAAGTTTGTAATCATCCTGATCTCTTTGAGGGTCGTCCTATTGTAAGTTCTTTTGATATGGCTGGCATTGATGTGCAGTTGAGTTCAACAATCTGTTCGCTACTATTGGAAAGTCCGTTTTCCAAGGTTGATCTTGAGGCTTTGGGATTCTTATTCACCCATCTTGATTTTTCTATGACTTCTTGGGAAGGAGATGAGATCAAAGCTATTTCAACACCCTCAGAATTAATCAAACAGCGTGTGAACTTGAAGGATGATCTAGAAGCAATCCCTTTGAGTCCAAAAAACCGCAAAAATCTGCAAGGGACAAATATTTTTGAAGAAATTCGTAAGGCTGTCTTTGAGGAAAGGATACAAGAATCTAAGGATCGGGCAGCAGCCATTGCATGGTGGAATTCTTTGAGATGCCAGAGAAAGCCGACTTACTCTACATCACTAAGAACTCTTTTAACCATTAAGGGTCCTCTTGATGATCTTAAAGCTAACTGTTCATCTTACATGTATTCATCAATCCTCGCTGACATAGTCCTTTCACCAATCGAGCGTTTTCAGAAGATGATTGAGCTAGTTGAAGCTTTCACATTTGCGATTCCAGCTGCACGAGTGCCTTCACCTACTTGCTGGTGCAGTAAAAGTGACTCTCCTGTTTTTCTTAGTCCATCGTACAAGGAAAAAGTTACAGATTTATTGTCACCTCTTTTATCTCCAATTAGACCTGCAATCGTTAGGAGACAAGTATATTTTCCAGATAGGCGACTGATACAGTTTGATTGTGGTAAGCTGCAGGAGCTTGCTATGTTATTGAGGAAATTAAAATTTGGGGGTCACAGAGCGTTGATATTCACGCAAATGACCAAGATGCTTGACGTCTTGGAGGCTTTCATTAATTTATATGGTTATACCTACATGCGTCTTGATGGCTCTACACCTCCAGAAGAGAGGCAGACGTTAATGCAGCGGTTTAATACAAATCCCAAGATCTTTCTTTTCATTTTGTCAACCCGAAGTGGGGGCGTTGGCATCAACCTTGTGGGTGCGGACACAGTTATATTCTATGACAGTGACTGGAATCCTGCTATGGATCAACAAGCTCAAGATCGCTGCCACAGGATAGGTCAGACACGTGAAGTGCATATATACCGGTTGATAAGTGAGAGCACCATTGAGGAGAATATCTTAAAGAAAGCAAACCAGAAGCGTGTGCTTGATAACCTAGTAATTCAAAACGGTGAGTATAACACCGAATTTTTCAAGAAGCTCGATCCCATGGAATTGTTCTCTGGGCATAAGGCTCTGACTACAAAGGATGAAAAGGAAACAAGCAAGCATTGTGGGGCGGATATACCTCTATCAAATGCAGATGTTGAGGCTGCTCTAAAACAGGCCGAAGATGAGGCAGATTATATGGCTCTCAAAAGAGTAGAACAAGAAGAAGCTGTGGACAATCAAGAGTTCACAGAAGAACCTGTAGAGAGACCAGAAGACGATGAGCTAGTAAATGAAGATGACATAAAGGCTGATGAGCCTGCTGATCAAGGTTTAGTTGCAGCAGGGCCGGCCAAAGAAGAAATGTCATTGTTACATAGTGATATCAGGGATGAGAGAGCTGTTATCACTACTTCAAGCCAAGAAGATGACACTGATGTATTAGATGATGTCAAGCAAATGGCTGCAGCAGCGGCTGATGCTGGACAAGCTATCTCATCTTTTGAGAATCAGCTACGTCCTATTGATAGATATGCGATTAGATTTTTAGAGCTTTGGGACCCCATCATTGTTGAGGCTGCAATGGAAAATGAAGCAGGATTTGAAGAGAAAGAATGGGAACTTGACCACATTGAGAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGATGGGGAAGAGCCTCTTGTTTACGAGAAATGGGATGCTGATTTTGCAACTGAGGCATACAGGCAGCAAGTGGAAGTCTTAGCTCAGCACCAGTTAATGGAAGATTTGGAAAATGAAGCTAGAGAGAGGGAAGCGGCAGAGGTGGCTGAAATGGTTTTGACACAGAACGAAAGTGCTCATGTACTGAAGCCTAAGAAGAAGAAAAAGGCGAAGAAAGCCAAATATAAATCCCTGAAGAAAGGATCACTGGCTGCCGAATCAAAACATGTGAAGTCAGTTGTGAAAATTGAAGATTCTACTGATGATGATAATGAAGAATTCGGCTATGTGAGTAGCAGTGACTCGGATATGGTTACACCGCTTTCGCGGATGCATATGAAGGGCAAGAAAAGAGATTTAATTGTTGACACTGATGAAGAGAAGACCTCAAAGAAGAAAGCAAAGAAGCACAAGAAGTCTCTCCCGAATTCAGATATCAAATATAAGCAAACAAGTGCACTACTTGATGAACTGGAACCATCAAAGCCGTCTGACAGTATGGTTGTTGATAATGAGTTGAAATTGACGAACAGGGGTAAAACTGTAGGGAAAAAATTCATCACTTCCATGCCAATAAAGCGTGTTCTTATGATCAAGCCAGAGAAATTGAAGAAAGGAAATCTGTGGTCTAGAGATTGTGTTCCCTCGCCTGATTCTTGGTTGCCGCAGGAAGATGCTATATTGTGTGCCATGGTTCATGAATATGGGCCTAACTGGAATTTTGTTAGCGGAACTCTTTATGGAATGACAGCTGGTGGGGCTTACAGAGGACGGTATCGGCATCCTGCATATTGTTGTGAGAGATATAGAGAGCTTATTCAACGACATATTTTGTCTGCTTCCGACAGTGCAGTGAACGAGAAGAACCTCAACACAGGTTCTGGGAAGGCTCTCCTTAAAGTCACTGAGGAAAATATTCGGACACTCCTAAATGTTGCAGCTGAGCAGCCAGATACGGAGATGCTCCTACAAAAACATTTTTCTTGCTTGCTGTCATCAATTTGGAGGACGTCTACCCGCACTGGGAATGATCAAATGCTCTCCTTAAACAGTCCTATTTTCAACAGACAGTTTATGGGTTCCGTGAATCACACCCAAGATTTAGCTAGAAAGCCTTGGCAGGGGATGAAGGTCACCAGCTTAAGTAGAAAGTTGTTAGAATCCGCTCTCCAGGATTCTGGCCCGAGTCAGCCAGACAATACTATTTCCCGTTCTAGGTTGCAGGAGACTCAACCTATCAATAAATTGGGATTAGAGTTAACCTTGGAATTCCCACGTGGCAATGATGATTCCCTGAATCAGTTTCCACCGATGATTAGTCTGTCCATTGATGGGTCAGATTCATTAAATTATGTGAACGAGCCCCCAGGGGAAGATGTTCTAAAAGGTTCTAGGGTTGCTGCAGAGAACCGTTACAGGAATGCTGCAAATGCTTGTATTGAAGATTCATTTGGATGGGCTTCAAACACGTTCCCAGCCAATGATCTGAAGTCAAGAACAGGCACGAAAGCACAGTCCCTGGGGAAACACAAGCTATCCGCCTCAGACTCAGCCAAGTCTACAAAAAGCAAACACCGCAAACTCTTAGCAGAGCAATTGGAAGGGGCATGGGTTAGGCCAAATGATCCGAATCTCAAGTTTGATTTCACACCTGGTGATAGGGAGGAGGAGGAGGAGCAGGAGGTGGATGAGAAAGCGAATTCAGCAGAGATAGAGATGATAAGCTGTTCACAATGGTATGATCCATTCTTTACCTCAGGCCTTGATGATTGCTCATTAGCTTCGGATATCTCAGAAATAGAGTAG

Protein Analysis

2055

Amino Acids

234.05

Weight (kDa)

5.21

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 40 - 106 1.5e-16 HSA domain
ResIII PF04851 534 - 695 2.2e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 539 - 824 1.8e-89 SNF2-related domain
DEAD PF00270 556 - 696 4.4e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1080 - 1188 4.3e-25 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 498
AasI GACNNNNNNGTC 1 cut(s) 1640
AatII GACGTC 2 cut(s) 3318, 5375
Acc36I ACCTGC 2 cut(s) 590, 3172
AccB1I GGYRCC 1 cut(s) 2271
AccB7I CCANNNNNTGG 3 cut(s) 154, 2835, 4420
AccBSI CCGCTC 2 cut(s) 1867, 5528
AccI GTMKAC 4 cut(s) 1353, 2332, 5375, 5921
AccII CGCG 1 cut(s) 4690
AclI AACGTT 1 cut(s) 1398
AclWI GGATC 8 cut(s) 762, 1418, 2823, 3537, 3704, 4573, 5981, 6098
AcoI YGGCCR 1 cut(s) 4005
AcuI CTGAAG 6 cut(s) 972, 981, 1359, 4529, 4577, 5870
AcvI CACGTG 2 cut(s) 3572, 5639
AcyI GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
AdeI CACNNNGTG 1 cut(s) 6017
AfaI GTAC 8 cut(s) 393, 461, 1260, 1616, 2080, 3113, 4295, 4506
AflII CTTAAG 2 cut(s) 1595, 5503
AflIII ACRYGT 5 cut(s) 2137, 2946, 3569, 4588, 5829
AgeI ACCGGT 1 cut(s) 3588
AhdI GACNNNNNGTC 1 cut(s) 187
AjiI CACGTC 1 cut(s) 125
AjnI CCWGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
AjuI GAANNNNNNNTTGG 4 cut(s) 1677, 1709, 5796, 5828
Alw21I GWGCWC 3 cut(s) 3608, 4501, 4822
Alw44I GTGCAC 1 cut(s) 4818
AlwI GGATC 8 cut(s) 762, 1418, 2823, 3537, 3704, 4573, 5981, 6098
AlwNI CAGNNNCTG 5 cut(s) 950, 3513, 4130, 5243, 5881
Ama87I CYCGRG 1 cut(s) 5545
ApaLI GTGCAC 1 cut(s) 4818
ArsI GACNNNNNNTTYG 2 cut(s) 5861, 5893
AseI ATTAAT 2 cut(s) 2675, 3333
AsiGI ACCGGT 1 cut(s) 3588
Asp700I GAANNNNTTC 4 cut(s) 2772, 4916, 5277, 5739
AspLEI GCGC 4 cut(s) 214, 1965, 2274, 2352
AspS9I GGNCC 8 cut(s) 145, 505, 1757, 2909, 4001, 4213, 5069, 5543
AsuII TTCGAA 2 cut(s) 1573, 2306
AvaI CYCGRG 1 cut(s) 5545
AvaII GGWCC 4 cut(s) 145, 1757, 2909, 4213
AxyI CCTNAGG 1 cut(s) 6118
BaeGI GKGCMC 1 cut(s) 4822
BanI GGYRCC 1 cut(s) 2271
BanII GRGCYC 1 cut(s) 5727
BarI GAAGNNNNNNTAC 6 cut(s) 741, 773, 1961, 1993, 2351, 2383
BauI CACGAG 1 cut(s) 3048
BbrPI CACGTG 2 cut(s) 3572, 5639
BbsI GAAGAC 4 cut(s) 104, 1347, 3936, 4751
Bbv12I GWGCWC 3 cut(s) 3608, 4501, 4822
BceAI ACGGC 3 cut(s) 1570, 2285, 4834
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BciT130I CCWGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
BciVI GTATCC 1 cut(s) 2793
BclI TGATCA 3 cut(s) 3979, 4953, 5392
BfoI RGCGCY 1 cut(s) 2275
BfrI CTTAAG 2 cut(s) 1595, 5503
BfuAI ACCTGC 2 cut(s) 590, 3172
BfuI GTATCC 1 cut(s) 2793
BglII AGATCT 2 cut(s) 1529, 3426
BlpI GCTNAGC 3 cut(s) 87, 4407, 5304
BmcAI AGTACT 1 cut(s) 1260
Bme1390I CCNGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
Bme18I GGWCC 4 cut(s) 145, 1757, 2909, 4213
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 5545
BmgBI CACGTC 1 cut(s) 125
BmgT120I GGNCC 8 cut(s) 145, 505, 1757, 2909, 4001, 4213, 5069, 5543
BmrFI CCNGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
BmrI ACTGGG 4 cut(s) 151, 812, 866, 5394
BmuI ACTGGG 4 cut(s) 151, 812, 866, 5394
BpiI GAAGAC 4 cut(s) 104, 1347, 3936, 4751
BplI GAGNNNNNCTC 6 cut(s) 446, 478, 1421, 1453, 4733, 4765
BpmI CTGGAG 3 cut(s) 564, 3363, 5516
Bpu1102I GCTNAGC 3 cut(s) 87, 4407, 5304
Bpu14I TTCGAA 2 cut(s) 1573, 2306
BpuEI CTTGAG 5 cut(s) 1058, 1859, 2603, 3525, 5981
BsaAI YACGTR 2 cut(s) 3572, 5639
BsaBI GATNNNNATC 4 cut(s) 639, 885, 2429, 5991
BsaHI GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
BsaI GGTCTC 3 cut(s) 653, 1013, 3916
BsaJI CCNNGG 9 cut(s) 2571, 3714, 5052, 5477, 5625, 5728, 5729, 5880, 5881
BsaWI WCCGGW 1 cut(s) 3588
BsaXI ACNNNNNCTCC 2 cut(s) 1793, 1823
Bse118I RCCGGY 2 cut(s) 3588, 4003
Bse21I CCTNAGG 1 cut(s) 6118
Bse3DI GCAATG 5 cut(s) 403, 1713, 2829, 4242, 5650
Bse8I GATNNNNATC 4 cut(s) 639, 885, 2429, 5991
BseBI CCWGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
BseDI CCNNGG 9 cut(s) 2571, 3714, 5052, 5477, 5625, 5728, 5729, 5880, 5881
BseJI GATNNNNATC 4 cut(s) 639, 885, 2429, 5991
BseMI GCAATG 5 cut(s) 403, 1713, 2829, 4242, 5650
BseRI GAGGAG 9 cut(s) 173, 572, 701, 782, 3629, 6041, 6044, 6047, 6050
BseSI GKGCMC 1 cut(s) 4822
BseYI CCCAGC 2 cut(s) 1536, 5836
BsgI GTGCAG 4 cut(s) 2543, 3030, 3094, 5232
Bsh1236I CGCG 1 cut(s) 4690
BshNI GGYRCC 1 cut(s) 2271
BshTI ACCGGT 1 cut(s) 3588
BsiHKAI GWGCWC 3 cut(s) 3608, 4501, 4822
BsiHKCI CYCGRG 1 cut(s) 5545
BsiSI CCGG 2 cut(s) 3589, 4004
BslFI GGGAC 6 cut(s) 158, 1743, 2217, 2767, 4226, 5863
BsmFI GGGAC 6 cut(s) 158, 1743, 2217, 2767, 4226, 5863
BsmI GAATGC 3 cut(s) 255, 1600, 5788
Bso31I GGTCTC 3 cut(s) 653, 1013, 3916
BsoBI CYCGRG 1 cut(s) 5545
Bsp119I TTCGAA 2 cut(s) 1573, 2306
Bsp1286I GDGCHC 4 cut(s) 3608, 4501, 4822, 5727
Bsp1720I GCTNAGC 3 cut(s) 87, 4407, 5304
Bsp19I CCATGG 2 cut(s) 3714, 5052
BspFNI CGCG 1 cut(s) 4690
BspHI TCATGA 1 cut(s) 5059
BspMAI CTGCAG 5 cut(s) 601, 2091, 3234, 4123, 5767
BspMI ACCTGC 2 cut(s) 590, 3172
BspPI GGATC 8 cut(s) 762, 1418, 2823, 3537, 3704, 4573, 5981, 6098
BspQI GCTCTTC 2 cut(s) 1725, 4326
BspT104I TTCGAA 2 cut(s) 1573, 2306
BspT107I GGYRCC 1 cut(s) 2271
BspTI CTTAAG 2 cut(s) 1595, 5503
BspTNI GGTCTC 3 cut(s) 653, 1013, 3916
BsrBI CCGCTC 2 cut(s) 1867, 5528
BsrDI GCAATG 5 cut(s) 403, 1713, 2829, 4242, 5650
BsrFI RCCGGY 2 cut(s) 3588, 4003
BssAI RCCGGY 2 cut(s) 3588, 4003
BssECI CCNNGG 9 cut(s) 2571, 3714, 5052, 5477, 5625, 5728, 5729, 5880, 5881
BssNI GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
BssSI CACGAG 1 cut(s) 3048
BssT1I CCWWGG 5 cut(s) 2571, 3714, 5052, 5477, 5625
Bst2BI CACGAG 1 cut(s) 3048
Bst2UI CCWGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
Bst6I CTCTTC 9 cut(s) 720, 790, 1170, 1209, 1725, 3378, 4254, 4326, 4734
BstACI GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
BstAFI CTTAAG 2 cut(s) 1595, 5503
BstAPI GCANNNNNTGC 3 cut(s) 1532, 1606, 2831
BstBAI YACGTR 2 cut(s) 3572, 5639
BstBI TTCGAA 2 cut(s) 1573, 2306
BstDSI CCRYGG 2 cut(s) 3714, 5052
BstEII GGTNACC 1 cut(s) 5494
BstFNI CGCG 1 cut(s) 4690
BstH2I RGCGCY 1 cut(s) 2275
BstHHI GCGC 4 cut(s) 214, 1965, 2274, 2352
BstNI CCWGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
BstNSI RCATGY 6 cut(s) 1740, 2141, 2321, 2950, 3358, 4592
BstPI GGTNACC 1 cut(s) 5494
BstSCI CCNGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
BstSLI GKGCMC 1 cut(s) 4822
BstUI CGCG 1 cut(s) 4690
BstV2I GAAGAC 4 cut(s) 104, 1347, 3936, 4751
BstX2I RGATCY 4 cut(s) 754, 1423, 1529, 3426
BstXI CCANNNNNNTGG 3 cut(s) 1544, 1625, 5540
BstYI RGATCY 4 cut(s) 754, 1423, 1529, 3426
Bsu36I CCTNAGG 1 cut(s) 6118
BsuI GTATCC 1 cut(s) 2793
BtgI CCRYGG 2 cut(s) 3714, 5052
BtrI CACGTC 1 cut(s) 125
BtsI GCAGTG 2 cut(s) 4666, 5220
BveI ACCTGC 2 cut(s) 590, 3172
CaiI CAGNNNCTG 5 cut(s) 950, 3513, 4130, 5243, 5881
CciI TCATGA 1 cut(s) 5059
CfoI GCGC 4 cut(s) 214, 1965, 2274, 2352
Cfr10I RCCGGY 2 cut(s) 3588, 4003
Cfr13I GGNCC 8 cut(s) 145, 505, 1757, 2909, 4001, 4213, 5069, 5543
CseI GACGC 4 cut(s) 33, 80, 109, 3347
CsiI ACCWGGT 1 cut(s) 6013
Csp6I GTAC 8 cut(s) 392, 460, 1259, 1615, 2079, 3112, 4294, 4505
CspAI ACCGGT 1 cut(s) 3588
CspCI CAANNNNNGTGG 2 cut(s) 3760, 3795
CviQI GTAC 8 cut(s) 392, 460, 1259, 1615, 2079, 3112, 4294, 4505
DinI GGCGCC 1 cut(s) 2273
DraI TTTAAA 1 cut(s) 1834
DraIII CACNNNGTG 1 cut(s) 6017
DrdI GACNNNNNNGTC 1 cut(s) 1640
DriI GACNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 1640
EaeI YGGCCR 1 cut(s) 4005
Eam1104I CTCTTC 9 cut(s) 720, 790, 1170, 1209, 1725, 3378, 4254, 4326, 4734
Eam1105I GACNNNNNGTC 1 cut(s) 187
EarI CTCTTC 9 cut(s) 720, 790, 1170, 1209, 1725, 3378, 4254, 4326, 4734
EciI GGCGGA 2 cut(s) 3800, 5890
Eco130I CCWWGG 5 cut(s) 2571, 3714, 5052, 5477, 5625
Eco147I AGGCCT 1 cut(s) 6123
Eco24I GRGCYC 1 cut(s) 5727
Eco31I GGTCTC 3 cut(s) 653, 1013, 3916
Eco32I GATATC 3 cut(s) 4039, 4798, 6151
Eco47I GGWCC 4 cut(s) 145, 1757, 2909, 4213
Eco57I CTGAAG 6 cut(s) 972, 981, 1359, 4529, 4577, 5870
Eco72I CACGTG 2 cut(s) 3572, 5639
Eco81I CCTNAGG 1 cut(s) 6118
Eco88I CYCGRG 1 cut(s) 5545
Eco91I GGTNACC 1 cut(s) 5494
EcoO109I RGGNCCY 3 cut(s) 1757, 2909, 4213
EcoO65I GGTNACC 1 cut(s) 5494
EcoRI GAATTC 6 cut(s) 2159, 2842, 4640, 4789, 5630, 6061
EcoRII CCWGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
EcoRV GATATC 3 cut(s) 4039, 4798, 6151
EcoT14I CCWWGG 5 cut(s) 2571, 3714, 5052, 5477, 5625
EcoT22I ATGCAT 2 cut(s) 1919, 4698
EcoT38I GRGCYC 1 cut(s) 5727
EgeI GGCGCC 1 cut(s) 2273
EheI GGCGCC 1 cut(s) 2273
ErhI CCWWGG 5 cut(s) 2571, 3714, 5052, 5477, 5625
FalI AAGNNNNNCTT 6 cut(s) 1248, 1280, 2771, 2803, 4323, 4355
FaqI GGGAC 6 cut(s) 158, 1743, 2217, 2767, 4226, 5863
FauI CCCGC 1 cut(s) 5387
FauNDI CATATG 1 cut(s) 4698
FbaI TGATCA 3 cut(s) 3979, 4953, 5392
FblI GTMKAC 4 cut(s) 1353, 2332, 5375, 5921
FriOI GRGCYC 1 cut(s) 5727
FseI GGCCGGCC 1 cut(s) 4007
GlaI GCGC 4 cut(s) 213, 1964, 2273, 2351
GsaI CCCAGC 2 cut(s) 1540, 5840
GsuI CTGGAG 3 cut(s) 564, 3363, 5516
HaeII RGCGCY 1 cut(s) 2275
HapII CCGG 2 cut(s) 3589, 4004
HgaI GACGC 4 cut(s) 33, 80, 109, 3347
HhaI GCGC 4 cut(s) 214, 1965, 2274, 2352
Hin1I GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
Hin6I GCGC 4 cut(s) 212, 1963, 2272, 2350
HinP1I GCGC 4 cut(s) 212, 1963, 2272, 2350
HincII GTYRAC 5 cut(s) 1120, 2073, 3447, 4729, 5622
HindII GTYRAC 5 cut(s) 1120, 2073, 3447, 4729, 5622
HindIII AAGCTT 4 cut(s) 735, 1895, 2410, 3021
HpaI GTTAAC 1 cut(s) 5622
HpaII CCGG 2 cut(s) 3589, 4004
Hpy99I CGWCG 2 cut(s) 129, 984
Hsp92I GRCGYC 5 cut(s) 5, 44, 2272, 3315, 5372
HspAI GCGC 4 cut(s) 212, 1963, 2272, 2350
KasI GGCGCC 1 cut(s) 2271
KflI GGGWCCC 2 cut(s) 1757, 4213
KroI GCCGGC 1 cut(s) 4003
KroNI GCCGGC 1 cut(s) 4005
Ksp22I TGATCA 3 cut(s) 3979, 4953, 5392
KspAI GTTAAC 1 cut(s) 5622
LguI GCTCTTC 2 cut(s) 1725, 4326
LmnI GCTCC 7 cut(s) 115, 490, 769, 1864, 3235, 5331, 6037
MabI ACCWGGT 1 cut(s) 6013
MbiI CCGCTC 2 cut(s) 1867, 5528
MfeI CAATTG 1 cut(s) 5957
MflI RGATCY 4 cut(s) 754, 1423, 1529, 3426
MhlI GDGCHC 4 cut(s) 3608, 4501, 4822, 5727
Mly113I GGCGCC 1 cut(s) 2272
MlyI GAGTC 9 cut(s) 197, 1337, 1349, 2734, 3080, 4658, 5557, 5584, 5903
MmeI TCCRAC 2 cut(s) 711, 5229
Mph1103I ATGCAT 2 cut(s) 1919, 4698
MroNI GCCGGC 1 cut(s) 4003
MroXI GAANNNNTTC 4 cut(s) 2772, 4916, 5277, 5739
MslI CAYNNNNRTG 8 cut(s) 1029, 1089, 2316, 2519, 3296, 4928, 5064, 5815
MspA1I CMGCKG 5 cut(s) 3044, 3406, 4127, 5114, 5303
MspCI CTTAAG 2 cut(s) 1595, 5503
MspI CCGG 2 cut(s) 3589, 4004
MspR9I CCNGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
MunI CAATTG 1 cut(s) 5957
Mva1269I GAATGC 3 cut(s) 255, 1600, 5788
MvaI CCWGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
MvnI CGCG 1 cut(s) 4690
NaeI GCCGGC 1 cut(s) 4005
NarI GGCGCC 1 cut(s) 2272
NcoI CCATGG 2 cut(s) 3714, 5052
NdeI CATATG 1 cut(s) 4698
NgoMIV GCCGGC 1 cut(s) 4003
NmeAIII GCCGAG 2 cut(s) 830, 884
NmuCI GTSAC 9 cut(s) 1642, 2112, 3083, 3138, 3272, 3509, 4661, 5263, 5494
NsiI ATGCAT 2 cut(s) 1919, 4698
NspI RCATGY 6 cut(s) 1740, 2141, 2321, 2950, 3358, 4592
NspV TTCGAA 2 cut(s) 1573, 2306
PacI TTAATTAA 1 cut(s) 2007
PagI TCATGA 1 cut(s) 5059
PasI CCCWGGG 2 cut(s) 5729, 5881
PceI AGGCCT 1 cut(s) 6123
PciI ACATGT 3 cut(s) 2137, 2946, 4588
PciSI GCTCTTC 2 cut(s) 1725, 4326
PcsI WCGNNNNNNNCGW 1 cut(s) 1583
PctI GAATGC 3 cut(s) 255, 1600, 5788
PdiI GCCGGC 1 cut(s) 4005
PdmI GAANNNNTTC 4 cut(s) 2772, 4916, 5277, 5739
PflFI GACNNNGTC 1 cut(s) 2972
PflMI CCANNNNNTGG 3 cut(s) 154, 2835, 4420
PfoI TCCNGGA 1 cut(s) 5532
PinAI ACCGGT 1 cut(s) 3588
PleI GAGTC 9 cut(s) 196, 1337, 1349, 2733, 3080, 4658, 5556, 5584, 5903
PluTI GGCGCC 1 cut(s) 2275
PmaCI CACGTG 2 cut(s) 3572, 5639
PmlI CACGTG 2 cut(s) 3572, 5639
PpsI GAGTC 9 cut(s) 196, 1337, 1349, 2733, 3080, 4658, 5556, 5584, 5903
Ppu21I YACGTR 2 cut(s) 3572, 5639
PpuMI RGGWCCY 3 cut(s) 1757, 2909, 4213
PscI ACATGT 3 cut(s) 2137, 2946, 4588
PshBI ATTAAT 2 cut(s) 2675, 3333
PsiI TTATAA 1 cut(s) 498
Psp1406I AACGTT 1 cut(s) 1398
Psp5II RGGWCCY 3 cut(s) 1757, 2909, 4213
Psp6I CCWGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
PspCI CACGTG 2 cut(s) 3572, 5639
PspEI GGTNACC 1 cut(s) 5494
PspFI CCCAGC 2 cut(s) 1536, 5836
PspGI CCWGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
PspPI GGNCC 8 cut(s) 145, 505, 1757, 2909, 4001, 4213, 5069, 5543
PspPPI RGGWCCY 3 cut(s) 1757, 2909, 4213
PstI CTGCAG 5 cut(s) 601, 2091, 3234, 4123, 5767
PstNI CAGNNNCTG 5 cut(s) 950, 3513, 4130, 5243, 5881
PsuI RGATCY 4 cut(s) 754, 1423, 1529, 3426
PsyI GACNNNGTC 1 cut(s) 2972
PvuII CAGCTG 3 cut(s) 3044, 5114, 5303
RigI GGCCGGCC 1 cut(s) 4007
RsaI GTAC 8 cut(s) 393, 461, 1260, 1616, 2080, 3113, 4295, 4506
RsaNI GTAC 8 cut(s) 392, 460, 1259, 1615, 2079, 3112, 4294, 4505
RseI CAYNNNNRTG 8 cut(s) 1029, 1089, 2316, 2519, 3296, 4928, 5064, 5815
SapI GCTCTTC 2 cut(s) 1725, 4326
Sau96I GGNCC 8 cut(s) 145, 505, 1757, 2909, 4001, 4213, 5069, 5543
ScaI AGTACT 1 cut(s) 1260
SchI GAGTC 9 cut(s) 197, 1337, 1349, 2734, 3080, 4658, 5557, 5584, 5903
ScrFI CCNGG 6 cut(s) 1044, 2156, 5534, 5730, 5882, 6015
SduI GDGCHC 4 cut(s) 3608, 4501, 4822, 5727
SexAI ACCWGGT 1 cut(s) 6013
SfoI GGCGCC 1 cut(s) 2273
SfuI TTCGAA 2 cut(s) 1573, 2306
SinI GGWCC 4 cut(s) 145, 1757, 2909, 4213
SmiMI CAYNNNNRTG 8 cut(s) 1029, 1089, 2316, 2519, 3296, 4928, 5064, 5815
SmlI CTYRAG 7 cut(s) 1037, 1595, 1874, 2582, 3540, 5503, 5996
SmoI CTYRAG 7 cut(s) 1037, 1595, 1874, 2582, 3540, 5503, 5996
SseBI AGGCCT 1 cut(s) 6123
SspDI GGCGCC 1 cut(s) 2271
SspI AATATT 2 cut(s) 2761, 5278
StuI AGGCCT 1 cut(s) 6123
StyD4I CCNGG 6 cut(s) 1042, 2154, 5532, 5728, 5880, 6013
StyI CCWWGG 5 cut(s) 2571, 3714, 5052, 5477, 5625
TaqII GACCGA 1 cut(s) 677
TatI WGTACW 3 cut(s) 1258, 4293, 4504
TauI GCSGC 3 cut(s) 4130, 4460, 5029
TseFI GTSAC 9 cut(s) 1642, 2112, 3083, 3138, 3272, 3509, 4661, 5263, 5494
Tsp45I GTSAC 9 cut(s) 1642, 2112, 3083, 3138, 3272, 3509, 4661, 5263, 5494
TspGWI ACGGA 4 cut(s) 2243, 2553, 5333, 5434
Tth111I GACNNNGTC 1 cut(s) 2972
Van91I CCANNNNNTGG 3 cut(s) 154, 2835, 4420
Vha464I CTTAAG 2 cut(s) 1595, 5503
VneI GTGCAC 1 cut(s) 4818
VpaK11BI GGWCC 4 cut(s) 145, 1757, 2909, 4213
VspI ATTAAT 2 cut(s) 2675, 3333
XbaI TCTAGA 2 cut(s) 2707, 4991
XceI RCATGY 6 cut(s) 1740, 2141, 2321, 2950, 3358, 4592
XmiI GTMKAC 4 cut(s) 1353, 2332, 5375, 5921
XmnI GAANNNNTTC 4 cut(s) 2772, 4916, 5277, 5739
ZraI GACGTC 2 cut(s) 3316, 5373
ZrmI AGTACT 1 cut(s) 1260
Zsp2I ATGCAT 2 cut(s) 1919, 4698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.