Rroxscaffold_1G00054140
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014066
Physical Location & Seq
Forward (+)
75471401 .. 75484107
12707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054140.1

Sequence Viewer

Length: 6129 bp
ATGGCTTCCAAAGGTCCAAGGCCGAAGCTCGATCACGAACCCAGACCCAAGCGCCACAAGGCCCTTGAAGTTGCGAATGAACCTCGACGGCCAAAAGCTCATTGGGACCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAATCGGAGAGAAAATGGAAATTGGCTCAGGCAAAGAAGGTTGCTTTACGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAAGAAAATGAAGGTGCTTTACAAACATCAGATGGAGCTTGATGAGAAAAAGAAAAAGGCGCTTGATAAACAGCTTGAGTTCCTTCTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGCGGACAAGCCTGTGCAGCAATGTTCCACACAAGATCCACTGAGTATTGAAGGCACAGCTAACCTCAATCAGATACAGCAGACGGGTGATGCTGATTATAATATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAAAGACGGGAAGAATTGGCTGCTTTACAGAATGAAGTGGATGTTCCACTCGAGGAGCTACTCAAACAATATGCTAGAAAGAAAGGGAGGTAGAGATAGGGAGTTCGATTTGTCACCGGTTTTACAAAAAGTTTCAGATTTAGTTAACACAGAAAGTAGTCAAGAAAAGGATGAAGATGATGTTGCAGAGACGACTTCTTCTGGGGAAGATGATGGTATATCTCCTGAAAAGGGTGAAGATGACACTGAAATGACCTCAGTGGGGAAAGATCATAGTATGAGTTCAGAAAAGGGCGAAGATGGTGCAGAAGTCATGTCTGTGGGGGAAGATCATGATATGTGTCATAAAACGGGTGAAGTTGGCGCAGAAATGACCTCATTGAGTGAAGATCATGGTGAACAAAACGATCTTCTTATAGCCTCGACATTCTCGTTTATCAATGTGTTTTATCTGATTGGGACCTTCACGTCTGGTGAACTCATTTCTGATTTATCTCTTAGCATGGAGGATGGTGACTTCATTCTTGCTGCTGGAGAAGAAAAGTTTACTGACTTGCTACCTTTATTTAAAGATGATGAGACAACCTTATTAGAGGAGGAGGAATTGGCAAAAGCAGATACCAATCATCCCATGGATGAGATTGCATTACTGCAGAAGGAGAGTGAAATACCGTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAGCGGTGATGAAGTTGCAGATGGTGAATCTGAATATGGTTCTGCTTTATCAGAGGGATTTACGGATTCTCCATCTCTAGAAGATTCTGAACTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCAACTGAAGAACATAGGGCTTCAGAAGGAGGAAGTGATAGTGAGAACAGAATCGAGGATGCAGCTGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACCTTCTCCACAACAAAAGTCCGTACAAAGTTCCCCTTTCTTCTGAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACAATGTATGAGAAAAGGCTGAATGGGATTCTAGCTGACGAGATGGGGCTAGGGAAGACAATCATGACAATTGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGCCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACGGAGTTTCTAAAATGGTGCCCTGCTTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGCAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCATTTCATGTCTGCATAACAACGTATAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCGCAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGTTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTCAGTAATCCAATATCTGGGATGGTTGAAGGGCAAGAAAAAGTAAATAAAGAAGTTCTTGATCGTCTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAACGAGATGTGGAGAAACAGCTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTTTCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGCTCAGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCATCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTTACATGCAACTGAGTTCTTCAATATGTTCAATGCTTTCACCTGGACCATTTTCTGTGGTAGACCTTAAGGGATTGGGGTTTTTATTTACTCATCTAGATTTTTCGATGTCTTCTTGGGAGAGTGATGAAGTTAAAGCTCTTACTACCCCATCAAGTTTAATTAAGGAGCGTGTTACCCGGATTCATCTTGAAGATATTGGTGGATATAAACATCGTAAACATCACAAGAAGATGCATGGTATGAATATCTTCGAAGACATCCAGAAGGCACTTATGGAGGAGAGGCTAAGGCAAGCAAAAGAACATGCTGCAGCCGTTGCATGGTGGAATTCCTTTAGGTGTGATAGAAAACCTATATACTCAACGAGCCTGAGGGATCTTATTACAGTTAGACATCCTGTTGTTGATATTGCTCATCACAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAGTTCTTTCACCAGTAGAACGCTTCCAGAAAACAATTGACCTGGTTGAAAGCTTCGTGTTTGCAATTCCAGCTGCACGAGCTGCCCCACCTGTATGCTGGTGCTGTAAATCTGAAAGTTCTGTGTTTCTGCAACCAACTTATAAGCAGAAATGCTCTGAAGTCTTGTCGCCACTTCTGTCACCATTTAGACCTGCAATTGTCCGTAGACAAGTATATTTTCCTGATAGGAGACTAATACAGTTTGACTGTGGTAAGTTGCAGCAGCTTGCAGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACGAAGATGCTTGACGTCTTGGAGGCTTTCATCAATTTATATGGTTACACATACATGCGTTTAGATGGATCCACTCCGCCTGAGGAGAGGCAGACTTTAATGCAGCGGTTTAACACAAACCCAAAATTTTTTCTTTTCATATTGTCAACTCGTAGTGGAGGTGTTGGTATTAACCTGGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGTTGCCACCGGATTGGACAGACACGGGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATTTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTAGTTATACAAAGTGGGGACTATAACACTGAATTCTTCAAAAAGCTTGATCCCATGGAGCTGTTTTCTGGTCATAGAGCGCTTCCCATTAAGAACATGCAAAAGGAGAAAAATCACAACACAACTGAAGTTTCTCTATCTAATGTGGACCTGGAAGCTGCACTAAAGCATGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTTGAGCAGGAAGAAGCTGTGGACAACCAAGAATTTACTGAAGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGACGACATGAAGGTTGATGAACCCACAGATCAGGGGGCTTTGATGATGATGTCTTTAAACAAAGACAGGGGAATGATGTTGAATGGGAGTGATCCTAATGAAGAAAGAGCTCTCACTGGTGCTTGTAGAGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCAGCCGCAGGACAAGAGATCTCATCCTTTGGAAATCAGTTACGTCCGATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAATAGACAAGACAGCAGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAACTGGACCGCATAGAAAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTTGTATATGAAACATGGGATGCTGAATTTGCAACTGAGGCATATCGGCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGAGAAGGAAGATGAAGCAGATGAAAATCTTGATTCTCTGAGGTATGGGATGCCAAGTGATCCTAAACCCAAGCATAAGAAGAAATCGAAGAAAGCAAAATTTAAATCTCTGAAGAAACGGTCTCTAGCTTCTGAGCTGGAACCTGTTAAAGGAGAGCTGCAAGTAGAACCGATGTTCATAGATGAAGATTATCTCTCTAATGATGTGTTTTCCTATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGCAAGAAGGCTGAATCTAAACCAGCTGGTGAAGATAAGATTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCGTGAAATCAGTACTTCAGAGTTTGATACTAGTGTCTCCTCAGTGCAGCATGTTGAAGCTTCAGAGTTGAAACCATGCGAGAGTGTGGTTGAATTCGAGCATAAACCAATTAGTAGAACCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCGGACTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGCTTGGTGAGTGAGACGCTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCATTGTTGTGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTCTCCGCTCCAGACAATCCTAATTATGAAAAGGTCAACAATATAGGATCTGGAAAAGCTCTTTTAAGAGTTACAGAGATGGCATCTCACAAAGACGGGAGACAAAATCTCCCATCTTCTGGGAATGGTGTATATTTTGGCGGAAATTTTTTCAGTTCTTCCAACCAAATTTCTCGGACTTCAGTGAAGGAAAACACAGCAACCATGAAATTTACCCATTTTGGACAGGGTGCCAGGTTGGTAGCAGCTGCACTTAATGATGCCAGCAGCAGGCGAGGGGATGAGTGTGTCATTTCTCCCAACCCTAGGAAAAAATCATCAACTGATGCTGAACAGTTGGATATAACTCTGGAGTTTCAGGCACAAAAGGATGACTCCACAGATCCATTTCCATCTGTTATCAATTTATCAATATCTGATATAGGCCCTCCATCAGAAAATAAGGCAACGGAACCGTATCTTCTCAGAGATTCCTGTAATGACTATTGCAAAAGCACCAGTGATGCAAATGTGGCTGAGAACCGTTTCAGGAATGCAACAAGAGCTTGCGATGAAGATACTATGGGGTGGGCGTCATCGACATTCCCGACATATGATGTCAGGTCTCGATCAGTGTCAAAGTTACCTTCCTCGGGAAAGCACAAGCTTGTCTTCTCTGATCCAGTTAGACCTTCAAAATCCAAGTTCAGAAAAAATGCAGTAGAGCATGGTGAGATGCGTCACTTTCTGGCTGAGCATGTGTTCCCACCCTTGCCAATAGCTGCACCGCTGAATCCCAGTCCAAGGTTCGACCTGAACCTACCTGTCAACGAGGATATTGAGACTGGTGATCTGGAAAGCACTTCCCATTCTCAGGTGGTTGAGTCTTCATTTGAAGAAAGCTTTGGAGTTCTCCCACATGAATATGTTCCTGAATTGCTTTCAGGCCTTGATGACTGTTCATTATTGCCAGAATATGACGACATTGCCTAA

Protein Analysis

2042

Amino Acids

232.75

Weight (kDa)

5.4

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 85 8.7e-12 HSA domain
ResIII PF04851 519 - 680 2.3e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 524 - 809 2.2e-89 SNF2-related domain
DEAD PF00270 541 - 681 7.4e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1065 - 1177 2.2e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 465, 3081
AasI GACNNNNNNGTC 1 cut(s) 1009
AatII GACGTC 1 cut(s) 3285
Acc36I ACCTGC 2 cut(s) 3139, 4345
AccB1I GGYRCC 2 cut(s) 1776, 5387
AccB7I CCANNNNNTGG 3 cut(s) 2144, 4384, 5276
AccBSI CCGCTC 2 cut(s) 1254, 5165
AccI GTMKAC 4 cut(s) 2194, 2287, 2532, 3145
AcoI YGGCCR 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 3282, 5729
AfaI GTAC 8 cut(s) 341, 1531, 2035, 2362, 2918, 4259, 4756, 5022
AfeI AGCGCT 1 cut(s) 3708
AflII CTTAAG 1 cut(s) 2537
AflIII ACRYGT 1 cut(s) 1351
AgeI ACCGGT 2 cut(s) 658, 2030
AhlI ACTAGT 1 cut(s) 4772
AjiI CACGTC 1 cut(s) 1011
AjnI CCWGG 6 cut(s) 2294, 2512, 2979, 3441, 3777, 5390
AjuI GAANNNNNNNTTGG 4 cut(s) 2434, 2466, 6024, 6056
AloI GAACNNNNNNTCC 2 cut(s) 1991, 2023
Alw21I GWGCWC 2 cut(s) 3575, 4030
AlwNI CAGNNNCTG 1 cut(s) 1475
Ama87I CYCGRG 2 cut(s) 592, 5788
Aor51HI AGCGCT 1 cut(s) 3708
AoxI GGCC 7 cut(s) 20, 60, 89, 1711, 5032, 5581, 6082
ApaI GGGCCC 1 cut(s) 1715
ArsI GACNNNNNNTTYG 4 cut(s) 1657, 1689, 4703, 4735
AsiGI ACCGGT 2 cut(s) 658, 2030
Asp700I GAANNNNTTC 3 cut(s) 2720, 5681, 6063
AspA2I CCTAGG 1 cut(s) 5462
AspLEI GCGC 5 cut(s) 54, 298, 908, 1824, 3709
AsuC2I CCSGG 1 cut(s) 2650
AsuII TTCGAA 1 cut(s) 2724
AsuNHI GCTAGC 1 cut(s) 2330
AvaI CYCGRG 2 cut(s) 592, 5788
AvaII GGWCC 9 cut(s) 14, 106, 1002, 1729, 2516, 3496, 3775, 4177, 4243
AvrII CCTAGG 1 cut(s) 5462
AxyI CCTNAGG 2 cut(s) 2843, 3348
BaeGI GKGCMC 2 cut(s) 1715, 1781
BaeI ACNNNNGTAYC 4 cut(s) 4761, 4794, 5012, 5045
BamHI GGATCC 1 cut(s) 3335
BanI GGYRCC 2 cut(s) 1776, 5387
BanII GRGCYC 2 cut(s) 1715, 4030
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BauI CACGAG 1 cut(s) 3015
BbsI GAAGAC 7 cut(s) 1661, 1903, 2325, 2574, 2733, 5800, 6015
Bbv12I GWGCWC 2 cut(s) 3575, 4030
BceAI ACGGC 3 cut(s) 104, 2211, 2771
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 6 cut(s) 2296, 2514, 2981, 3443, 3779, 5392
BclI TGATCA 1 cut(s) 3561
BcnI CCSGG 1 cut(s) 2650
BcuI ACTAGT 1 cut(s) 4772
BfmI CTRYAG 5 cut(s) 1193, 2042, 2284, 2781, 4089
BfoI RGCGCY 3 cut(s) 55, 299, 3710
BfrI CTTAAG 1 cut(s) 2537
BfuAI ACCTGC 2 cut(s) 3139, 4345
BglII AGATCT 1 cut(s) 4110
BlnI CCTAGG 1 cut(s) 5462
BlpI GCTNAGC 1 cut(s) 5889
BmcAI AGTACT 1 cut(s) 4756
Bme1390I CCNGG 7 cut(s) 2296, 2514, 2650, 2981, 3443, 3779, 5392
Bme18I GGWCC 9 cut(s) 14, 106, 1002, 1729, 2516, 3496, 3775, 4177, 4243
BmeT110I CYCGRG 2 cut(s) 592, 5788
BmgBI CACGTC 1 cut(s) 1011
BmrFI CCNGG 7 cut(s) 2296, 2514, 2650, 2981, 3443, 3779, 5392
BmrI ACTGGG 1 cut(s) 5928
BmtI GCTAGC 1 cut(s) 2334
BmuI ACTGGG 1 cut(s) 5928
BpiI GAAGAC 7 cut(s) 1661, 1903, 2325, 2574, 2733, 5800, 6015
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 3 cut(s) 1095, 5151, 5528
Bpu10I CCTNAGC 3 cut(s) 174, 2759, 3218
Bpu1102I GCTNAGC 1 cut(s) 5889
Bpu14I TTCGAA 1 cut(s) 2724
BpuEI CTTGAG 3 cut(s) 332, 3492, 4194
BpuMI CCSGG 1 cut(s) 2650
Bsa29I ATCGAT 1 cut(s) 3556
BsaBI GATNNNNATC 2 cut(s) 1164, 4434
BsaHI GRCGYC 2 cut(s) 3282, 5729
BsaI GGTCTC 3 cut(s) 2073, 4536, 5766
BsaJI CCNNGG 7 cut(s) 17, 1173, 3681, 4407, 5462, 5787, 5939
BsaWI WCCGGW 4 cut(s) 658, 2030, 3522, 4975
Bse118I RCCGGY 2 cut(s) 658, 2030
Bse21I CCTNAGG 2 cut(s) 2843, 3348
Bse3DI GCAATG 2 cut(s) 392, 6120
Bse8I GATNNNNATC 2 cut(s) 1164, 4434
BseBI CCWGG 6 cut(s) 2296, 2514, 2981, 3443, 3779, 5392
BseCI ATCGAT 1 cut(s) 3556
BseDI CCNNGG 7 cut(s) 17, 1173, 3681, 4407, 5462, 5787, 5939
BseJI GATNNNNATC 2 cut(s) 1164, 4434
BseMI GCAATG 2 cut(s) 392, 6120
BseRI GAGGAG 9 cut(s) 610, 1151, 1154, 2028, 2765, 2911, 3365, 4238, 4771
BseSI GKGCMC 2 cut(s) 1715, 1781
BsgI GTGCAG 8 cut(s) 401, 867, 2997, 3771, 4676, 4808, 5391, 5904
BshFI GGCC 7 cut(s) 22, 62, 91, 1713, 5034, 5583, 6084
BshNI GGYRCC 2 cut(s) 1776, 5387
BshTI ACCGGT 2 cut(s) 658, 2030
BshVI ATCGAT 1 cut(s) 3556
BsiHKAI GWGCWC 2 cut(s) 3575, 4030
BsiHKCI CYCGRG 2 cut(s) 592, 5788
BsiSI CCGG 5 cut(s) 659, 2031, 2650, 3523, 4976
BslFI GGGAC 5 cut(s) 119, 1015, 1715, 3659, 4190
BsmBI CGTCTC 3 cut(s) 725, 1751, 5051
BsmFI GGGAC 5 cut(s) 119, 1015, 1715, 3659, 4190
BsmI GAATGC 1 cut(s) 5695
BsnI GGCC 7 cut(s) 22, 62, 91, 1713, 5034, 5583, 6084
Bso31I GGTCTC 3 cut(s) 2073, 4536, 5766
BsoBI CYCGRG 2 cut(s) 592, 5788
Bsp119I TTCGAA 1 cut(s) 2724
Bsp120I GGGCCC 1 cut(s) 1711
Bsp1286I GDGCHC 4 cut(s) 1715, 1781, 3575, 4030
Bsp1720I GCTNAGC 1 cut(s) 5889
Bsp19I CCATGG 2 cut(s) 1173, 3681
BspANI GGCC 7 cut(s) 22, 62, 91, 1713, 5034, 5583, 6084
BspDI ATCGAT 1 cut(s) 3556
BspHI TCATGA 2 cut(s) 874, 1662
BspMAI CTGCAG 3 cut(s) 1197, 2785, 4093
BspMI ACCTGC 2 cut(s) 3139, 4345
BspOI GCTAGC 1 cut(s) 2334
BspT104I TTCGAA 1 cut(s) 2724
BspT107I GGYRCC 2 cut(s) 1776, 5387
BspTI CTTAAG 1 cut(s) 2537
BspTNI GGTCTC 3 cut(s) 2073, 4536, 5766
BsrBI CCGCTC 2 cut(s) 1254, 5165
BsrDI GCAATG 2 cut(s) 392, 6120
BsrFI RCCGGY 2 cut(s) 658, 2030
BssAI RCCGGY 2 cut(s) 658, 2030
BssECI CCNNGG 7 cut(s) 17, 1173, 3681, 4407, 5462, 5787, 5939
BssNI GRCGYC 2 cut(s) 3282, 5729
BssSI CACGAG 1 cut(s) 3015
BssT1I CCWWGG 6 cut(s) 17, 1173, 3681, 4407, 5462, 5939
Bst2BI CACGAG 1 cut(s) 3015
Bst2UI CCWGG 6 cut(s) 2296, 2514, 2981, 3443, 3779, 5392
Bst6I CTCTTC 3 cut(s) 114, 1566, 1826
BstACI GRCGYC 2 cut(s) 3282, 5729
BstAFI CTTAAG 1 cut(s) 2537
BstAPI GCANNNNNTGC 3 cut(s) 1475, 2789, 3020
BstBI TTCGAA 1 cut(s) 2724
BstDSI CCRYGG 2 cut(s) 1173, 3681
BstENI CCTNNNNNAGG 1 cut(s) 4557
BstH2I RGCGCY 3 cut(s) 55, 299, 3710
BstHHI GCGC 5 cut(s) 54, 298, 908, 1824, 3709
BstNI CCWGG 6 cut(s) 2296, 2514, 2981, 3443, 3779, 5392
BstSCI CCNGG 7 cut(s) 2294, 2512, 2648, 2979, 3441, 3777, 5390
BstSFI CTRYAG 5 cut(s) 1193, 2042, 2284, 2781, 4089
BstSLI GKGCMC 2 cut(s) 1715, 1781
BstV2I GAAGAC 7 cut(s) 1661, 1903, 2325, 2574, 2733, 5800, 6015
BstX2I RGATCY 6 cut(s) 400, 2848, 3335, 4110, 5204, 5539
BstXI CCANNNNNNTGG 1 cut(s) 3527
BstYI RGATCY 6 cut(s) 400, 2848, 3335, 4110, 5204, 5539
Bsu15I ATCGAT 1 cut(s) 3556
Bsu36I CCTNAGG 2 cut(s) 2843, 3348
BsuRI GGCC 7 cut(s) 22, 62, 91, 1713, 5034, 5583, 6084
BsuTUI ATCGAT 1 cut(s) 3556
BtgI CCRYGG 2 cut(s) 1173, 3681
BtgZI GCGATG 1 cut(s) 5721
BtrI CACGTC 1 cut(s) 1011
BtsI GCAGTG 1 cut(s) 4887
BveI ACCTGC 2 cut(s) 3139, 4345
CaiI CAGNNNCTG 1 cut(s) 1475
CciI TCATGA 2 cut(s) 874, 1662
CfoI GCGC 5 cut(s) 54, 298, 908, 1824, 3709
Cfr10I RCCGGY 2 cut(s) 658, 2030
ClaI ATCGAT 1 cut(s) 3556
CseI GACGC 4 cut(s) 2025, 5068, 5718, 5864
CsiI ACCWGGT 2 cut(s) 2979, 3441
Csp6I GTAC 8 cut(s) 340, 1530, 2034, 2361, 2917, 4258, 4755, 5021
CspAI ACCGGT 2 cut(s) 658, 2030
CviQI GTAC 8 cut(s) 340, 1530, 2034, 2361, 2917, 4258, 4755, 5021
DraI TTTAAA 3 cut(s) 1111, 3975, 4513
DrdI GACNNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 1009
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 114, 1566, 1826
EarI CTCTTC 3 cut(s) 114, 1566, 1826
EciI GGCGGA 4 cut(s) 2198, 2213, 3333, 5313
Ecl136II GAGCTC 1 cut(s) 4028
Eco130I CCWWGG 6 cut(s) 17, 1173, 3681, 4407, 5462, 5939
Eco147I AGGCCT 1 cut(s) 6084
Eco24I GRGCYC 2 cut(s) 1715, 4030
Eco31I GGTCTC 3 cut(s) 2073, 4536, 5766
Eco32I GATATC 1 cut(s) 5102
Eco47I GGWCC 9 cut(s) 14, 106, 1002, 1729, 2516, 3496, 3775, 4177, 4243
Eco47III AGCGCT 1 cut(s) 3708
Eco53kI GAGCTC 1 cut(s) 4028
Eco81I CCTNAGG 2 cut(s) 2843, 3348
Eco88I CYCGRG 2 cut(s) 592, 5788
EcoICRI GAGCTC 1 cut(s) 4028
EcoNI CCTNNNNNAGG 1 cut(s) 4557
EcoO109I RGGNCCY 6 cut(s) 61, 1002, 1711, 1712, 4177, 5582
EcoRI GAATTC 3 cut(s) 2800, 3659, 4835
EcoRII CCWGG 6 cut(s) 2294, 2512, 2979, 3441, 3777, 5390
EcoRV GATATC 1 cut(s) 5102
EcoT14I CCWWGG 6 cut(s) 17, 1173, 3681, 4407, 5462, 5939
EcoT22I ATGCAT 2 cut(s) 2079, 2709
EcoT38I GRGCYC 2 cut(s) 1715, 4030
ErhI CCWWGG 6 cut(s) 17, 1173, 3681, 4407, 5462, 5939
Esp3I CGTCTC 3 cut(s) 725, 1751, 5051
FalI AAGNNNNNCTT 8 cut(s) 1526, 1558, 2169, 2201, 4287, 4319, 5792, 5824
FaqI GGGAC 5 cut(s) 119, 1015, 1715, 3659, 4190
FauNDI CATATG 2 cut(s) 1706, 5749
FbaI TGATCA 1 cut(s) 3561
FblI GTMKAC 4 cut(s) 2194, 2287, 2532, 3145
FriOI GRGCYC 2 cut(s) 1715, 4030
GlaI GCGC 5 cut(s) 53, 297, 907, 1823, 3708
GsuI CTGGAG 3 cut(s) 1095, 5151, 5528
HaeII RGCGCY 3 cut(s) 55, 299, 3710
HaeIII GGCC 7 cut(s) 22, 62, 91, 1713, 5034, 5583, 6084
HapII CCGG 5 cut(s) 659, 2031, 2650, 3523, 4976
HgaI GACGC 4 cut(s) 2025, 5068, 5718, 5864
HhaI GCGC 5 cut(s) 54, 298, 908, 1824, 3709
Hin1I GRCGYC 2 cut(s) 3282, 5729
Hin6I GCGC 5 cut(s) 52, 296, 906, 1822, 3707
HinP1I GCGC 5 cut(s) 52, 296, 906, 1822, 3707
HincII GTYRAC 6 cut(s) 688, 1375, 2028, 3414, 5194, 5965
HindII GTYRAC 6 cut(s) 688, 1375, 2028, 3414, 5194, 5965
HindIII AAGCTT 6 cut(s) 2927, 2989, 3671, 4800, 5801, 6037
HpaI GTTAAC 2 cut(s) 688, 2028
HpaII CCGG 5 cut(s) 659, 2031, 2650, 3523, 4976
Hpy99I CGWCG 2 cut(s) 90, 2019
HpyCH4IV ACGT 4 cut(s) 1010, 1880, 3282, 4135
HpySE526I ACGT 4 cut(s) 1010, 1880, 3282, 4135
Hsp92I GRCGYC 2 cut(s) 3282, 5729
HspAI GCGC 5 cut(s) 52, 296, 906, 1822, 3707
KflI GGGWCCC 1 cut(s) 4177
Ksp22I TGATCA 1 cut(s) 3561
KspAI GTTAAC 2 cut(s) 688, 2028
LmnI GCTCC 6 cut(s) 271, 597, 2638, 3685, 5043, 5170
MabI ACCWGGT 2 cut(s) 2979, 3441
MaeII ACGT 4 cut(s) 1010, 1880, 3282, 4135
MbiI CCGCTC 2 cut(s) 1254, 5165
MfeI CAATTG 5 cut(s) 1668, 1750, 2442, 2973, 3135
MflI RGATCY 6 cut(s) 400, 2848, 3335, 4110, 5204, 5539
MhlI GDGCHC 4 cut(s) 1715, 1781, 3575, 4030
MlyI GAGTC 5 cut(s) 1370, 3610, 4363, 5525, 6029
MmeI TCCRAC 3 cut(s) 1433, 5343, 5475
Mph1103I ATGCAT 2 cut(s) 2079, 2709
MroXI GAANNNNTTC 3 cut(s) 2720, 5681, 6063
MspA1I CMGCKG 7 cut(s) 1472, 2254, 3011, 3373, 4691, 5405, 5926
MspCI CTTAAG 1 cut(s) 2537
MspI CCGG 5 cut(s) 659, 2031, 2650, 3523, 4976
MspR9I CCNGG 7 cut(s) 2296, 2514, 2650, 2981, 3443, 3779, 5392
MunI CAATTG 5 cut(s) 1668, 1750, 2442, 2973, 3135
Mva1269I GAATGC 1 cut(s) 5695
MvaI CCWGG 6 cut(s) 2296, 2514, 2981, 3443, 3779, 5392
NciI CCSGG 1 cut(s) 2650
NcoI CCATGG 2 cut(s) 1173, 3681
NdeI CATATG 2 cut(s) 1706, 5749
NheI GCTAGC 1 cut(s) 2330
NmuCI GTSAC 6 cut(s) 654, 1055, 3117, 3239, 3476, 5876
NsiI ATGCAT 2 cut(s) 2079, 2709
NspV TTCGAA 1 cut(s) 2724
PacI TTAATTAA 1 cut(s) 2634
PaeI GCATGC 2 cut(s) 214, 3800
PaeR7I CTCGAG 1 cut(s) 592
PagI TCATGA 2 cut(s) 874, 1662
PceI AGGCCT 1 cut(s) 6084
PciI ACATGT 1 cut(s) 1351
PcsI WCGNNNNNNNCGW 2 cut(s) 971, 5741
PctI GAATGC 1 cut(s) 5695
PdmI GAANNNNTTC 3 cut(s) 2720, 5681, 6063
PflMI CCANNNNNTGG 3 cut(s) 2144, 4384, 5276
PfoI TCCNGGA 1 cut(s) 2294
PinAI ACCGGT 2 cut(s) 658, 2030
PleI GAGTC 5 cut(s) 1370, 3610, 4363, 5525, 6028
PpsI GAGTC 5 cut(s) 1370, 3610, 4363, 5525, 6028
PpuMI RGGWCCY 2 cut(s) 1002, 4177
PscI ACATGT 1 cut(s) 1351
PsiI TTATAA 2 cut(s) 465, 3081
Psp124BI GAGCTC 1 cut(s) 4030
Psp5II RGGWCCY 2 cut(s) 1002, 4177
Psp6I CCWGG 6 cut(s) 2294, 2512, 2979, 3441, 3777, 5390
PspGI CCWGG 6 cut(s) 2294, 2512, 2979, 3441, 3777, 5390
PspOMI GGGCCC 1 cut(s) 1711
PspPPI RGGWCCY 2 cut(s) 1002, 4177
PspXI VCTCGAGB 1 cut(s) 592
PsrI GAACNNNNNNTAC 2 cut(s) 4893, 4925
PstI CTGCAG 3 cut(s) 1197, 2785, 4093
PstNI CAGNNNCTG 1 cut(s) 1475
PsuI RGATCY 6 cut(s) 400, 2848, 3335, 4110, 5204, 5539
PvuII CAGCTG 5 cut(s) 1472, 2254, 3011, 4691, 5405
RsaI GTAC 8 cut(s) 341, 1531, 2035, 2362, 2918, 4259, 4756, 5022
RsaNI GTAC 8 cut(s) 340, 1530, 2034, 2361, 2917, 4258, 4755, 5021
SacI GAGCTC 1 cut(s) 4030
ScaI AGTACT 1 cut(s) 4756
SchI GAGTC 5 cut(s) 1370, 3610, 4363, 5525, 6029
ScrFI CCNGG 7 cut(s) 2296, 2514, 2650, 2981, 3443, 3779, 5392
SduI GDGCHC 4 cut(s) 1715, 1781, 3575, 4030
SexAI ACCWGGT 2 cut(s) 2979, 3441
SfcI CTRYAG 5 cut(s) 1193, 2042, 2284, 2781, 4089
Sfr274I CTCGAG 1 cut(s) 592
SfuI TTCGAA 1 cut(s) 2724
SinI GGWCC 9 cut(s) 14, 106, 1002, 1729, 2516, 3496, 3775, 4177, 4243
SlaI CTCGAG 1 cut(s) 592
SmiI ATTTAAAT 1 cut(s) 4513
SmlI CTYRAG 5 cut(s) 311, 592, 2537, 3507, 4209
SmoI CTYRAG 5 cut(s) 311, 592, 2537, 3507, 4209
SpeI ACTAGT 1 cut(s) 4772
SphI GCATGC 2 cut(s) 214, 3800
SseBI AGGCCT 1 cut(s) 6084
SstI GAGCTC 1 cut(s) 4030
StuI AGGCCT 1 cut(s) 6084
StyD4I CCNGG 7 cut(s) 2294, 2512, 2648, 2979, 3441, 3777, 5390
StyI CCWWGG 6 cut(s) 17, 1173, 3681, 4407, 5462, 5939
SwaI ATTTAAAT 1 cut(s) 4513
TaiI ACGT 4 cut(s) 1013, 1883, 3285, 4138
TatI WGTACW 3 cut(s) 2916, 4257, 4754
TauI GCSGC 1 cut(s) 4100
TseFI GTSAC 6 cut(s) 654, 1055, 3117, 3239, 3476, 5876
Tsp45I GTSAC 6 cut(s) 654, 1055, 3117, 3239, 3476, 5876
TspGWI ACGGA 5 cut(s) 1328, 1517, 1775, 3131, 5621
Van91I CCANNNNNTGG 3 cut(s) 2144, 4384, 5276
Vha464I CTTAAG 1 cut(s) 2537
VpaK11BI GGWCC 9 cut(s) 14, 106, 1002, 1729, 2516, 3496, 3775, 4177, 4243
XagI CCTNNNNNAGG 1 cut(s) 4557
XbaI TCTAGA 4 cut(s) 1327, 2566, 4165, 4955
XcmI CCANNNNNNNNNTGG 4 cut(s) 99, 1171, 3033, 3889
XhoI CTCGAG 1 cut(s) 592
XmaJI CCTAGG 1 cut(s) 5462
XmiI GTMKAC 4 cut(s) 2194, 2287, 2532, 3145
XmnI GAANNNNTTC 3 cut(s) 2720, 5681, 6063
ZraI GACGTC 1 cut(s) 3283
ZrmI AGTACT 1 cut(s) 4756
Zsp2I ATGCAT 2 cut(s) 2079, 2709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.