Rh5BG178100
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014066
Physical Location & Seq
Forward (+)
19330839 .. 19347534
16696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG178100.1

Sequence Viewer

Length: 7554 bp
ATGATTAACAGCATTCCGATGATCAACTTATTGTGCTTGCTCATCAGTTTCTCAATCTTGTGGACTTGTCATAGTGCAGCACTGTTCATTGGGGACACGCTTAGGCCAGGAGAGGCTCTCAATTCCTCAAGCTTTTTGGTCTCTGCTTCGGGGGAGTTTACGTTGGGTTTCAACTCCGGCTACCTCACTCTGAGGTCCAAAAACTATACGGTTTGGGTTGGCAACCTGTACAGACGGATTTCAGACCCTTCGAAGGCACTTCTCACCTTGGACAAGAACAGAACTCTCCTGCAAATTTCCGGTATGGAGCGGTATGGCAACGTGATCATTTACTTCTCTCCGGATAATAGTACCAATGTTGTGGCTACCCTGTCGGATTCTGGCAATTTAGTCCTACAAGAGGTGGATCAGGATGTTTTTAACTCTCCTAAACAACAATCACCGTTAAAGAGGGTGTTGTGGCAAAGCTTTGACTATCCTAGTGTGGCTCTTATGCCGGAGATGCAATTAGGTGAATATCAGAGGGATCGGCAACCATTGTACATTTCGTCGCGATCGCTTTGGTCTTGGAAGGAAAGTCAGAACAGCCCAGGACTAGGGCCTTTCACCCTTGATTGGGATTCCGACACGCGCCAATTGGAAGTTAGCAGAGACGGAGATCTTTGGTGGACCAGCGGAGAATTAAGATATGGGAAATTTGAGTTTATTAATATCCCTGATGAATCCAATGTCAGTTACAGTTTTACCGTGGTTTCGAATGAGAAAGAAGAGTTCTTCAGTTACAATTACGCTGCCGTTGGTGATCAAAATGGGAGATCAGTCTGGGTGCTAGACACAGGTGGAGAGCTTTATGATGTTAATTATAACATTTCTATTGCGCGAGCTGGTGACTGTGATGGCTATAATACCAATGGAGGGTGCAAGAGACGGAGAGGCCTAAATCTACATCCAATCAATAAGCTCAATAAGCTCAATAGGTGGATAGGGATTGGTACTGCAATTGCGGTTGTTCTTCTGGCACTGGTGCTTTGCAGCATATGCTACTTAATACGAAGAAGAAAACTCGCAGGTGAGAACCAAAAAAGAATCCAGGATATGCTGAACATGATGAATTCCAATAGACCTACTAATGCTAATGGACTTCAAAATGATGGAAAGGGGGGACATGATCTAAGCGTATTTAACACCATGGCTTCCAAAGGTCCAAGGCCGAAGCTCGATCACGAACCCAGACCCAAGCGCCACAAGGCCCTTGAAGTTGCGAATGAACCTCGACGGCCAAAAGCTCATTGGGACCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAGTCGGAGAGAAAATGGAAATTGGCTCAGGCAAAGAAGGTTGCTTTACGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAAGAAAATGAAGGAAGAAGAGCAACGGATGAAAAAAGTTGCACTTAATATTTCAAAGGATGTGAAGAAATTCTGGCTGAAAATAGAAAAACTGGTGCTTTACAAACATCAGATGGAGCTTGATGAGAAAAAGAAAAAGGCGCTTGATAAACAGCTTGAGTTCCTTTTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGCGGACAAGCCTGTGCAGCAATGTTCCACACAAGATCATCTGAGTATTGAAGGCACAGCTGTGGGCGAAAATGATATTAGCAAATCAGCAGAATTGAATGTCGAACCTCAATCAGATACAGCAGACGGTGATGATGATTATAATATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAGACGGGAAGAATTAGCTGCTTTACAGAATGAAGTGGATGTTCCACTCGAGAAGCTACTCAAACAATATACTAGAAAGAAAGTTAACAGAGAAAGTAGTCAAGAAAAGGATGAAGATTATGTTGCAGAGATGACTTCTTCTGGGGAAGATGATGGTATATCTCCTGAAAAGGGTGAAGATGACACTGAAATGACCTCAGTGGGGAAAGATCATAGTATGAGTTCAGAAAAGGGTGAAGATGGTGCAGAAGTCATGTCTGTGGGGGAAGATCATGATATGTGTCATAAAACGGGTGAAGTTGGCGCAGAAATGACCTCATTGAGTGAAGATCATGGTGAACAAAACGATCTTCTTATAGCCAGTAAGACTGATAGAAGCAGCCCAGATGTTTTTACCGGTCGTCGTTGTGTTGGAAGTAATGGTGTGCCTATTTCAGAAACCCATTCATCCGAAATTAAGATTGGTGAAGCCAAAAATATTTCAGAAGCATCTCTGCAGTCTGCCAAAGGAAATATGCCATATGATTTTGATGATGAACATGAGGATGGTGACTTCATTCTTGCTGCTGGAGAAGAAAAGGATGATGAGACAACCTTATTAGAGGAGGAGGAATTGGCAAAAGCAGATACCAATCATCCCATGGATGAGATTGCGTTACTGCAGAAGGAGAGTGAAATACCGTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAGCGGTGATGAAGTTGCAGATGGTGAATCTGAATATGGTTCTGCTTTATCAGAGGGATTTACGGATTCTCCATCTCTAGAAGATTCTGAACTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCAACTGAAGAACATAGGGCTTCAGAAGGAGGAAGTGATAGTGAGAACAGAATCGAGGATGCAGCTGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACCTTCTCCACAACAAAAGTCCGTACAAAGTTCCCCTTTCTTCTGAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACAATGTATGAGAAAAGGCTGAATGGGATTCTAGCTGACGAGATGGGGCTAGGGAAGACAATCATGACAATCGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGCCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACTGAGTTTCTAAAATGGTGCCCTGCTTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGCAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCATTTCATGTCTGCATAACAACATATAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCGCAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGTTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTCAGTAATCCAATATCCGGGATGGTTGAGGGGCAAGAAAAAGTAAATAAAGAAGTTCTTGATCGTCTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAACGAGATGTGGAGAAACAGCTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTTTCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGCTCAGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCATCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTTACATGCAACTGAGTTCTTCAATATGTTCAATGCTTTCGCCTGGACCATTTTCTGTGGTAGACCTTAAGGGATTGGGGTTTTTATTTACTCATCTAGATTTTTCGATGTCTTCTTGGGAGAGTGATGAAGTTAAAGCTCTTGCTACCCCATCAAGTTTAATTAAAGAGCGTGTTACCCGGATTCATCTTGAAGATATTGGTGGATTTAAACATCGTAAACATCACAAGAAGATGCATGGTATGAATATCTTCGAAGACATCCAGAAGGCACTTATGGAGGAGAGACTAAGGCAAGCAAAAGAACATGCTGCAGCCGTTGCATGGTGGAATTCCTTGAGGTGTGATAGAAAACCTATATACTCAACGAGCCTGAGGGATCTTGTTACAGTTAGACATCCTGTTGTTGATATTGCTCATCACAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAGTTCTTTCACCAGTAGAACGCTTCCAGAAAACAATTGACCTGGTTGAAAGCTTCGTGTTTGCAATTCCAGCTGCACGAGCTGCCCCACCTGTATGCTGGTGCAGTAAATCTGAAAGTTCTGTGTTTCTGCAACCAACTTATAAGCAGAAATGCTCTGAAGTCTTGTCGCCACTTCTGTCACCATTTAGACCTGCAATTGTCCGTAGACAAGTATATTTTCCTGATAGGAGACTAATACAGTTTGACTGTGGTAAGTTGCAGCAGCTTGCAGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACGAAGATGCTTGACGTCTTGGAGGCTTTCATCAATTTATATGGTTACACGTACATGCGTTTAGATGGATCCACTCCGCCTGAGGAGAGGCAGACTTTAATGCAGCGGTTTAATACAAACCCAAAATTTTTTCTTTTCATATTGTCAACTCGTAGTGGAGGTGTTGGTATTAACCTAGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGTTGCCACCGGATTGGACAGACACGAGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATTTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTAGTTATACAAAGTGGGGACTATAACACTGAATTCTTCAAAAAGCTTGATCCCATGGAGCTGTTTTCTGGTCATAGAGCGCTTCCCATTAAGAACATGCAAAAGGAGAAAAATCACAACACAACTGAAGTTTCTCTATCTAATGTGGACCTGGAAGCTGCACTAAAGCATGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTTGAGCAGGAAGAAGCTGTGGACAACCAAGAATTTACTGAAGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGATGACATGAAGGTTGATGAACCCACAGATCAGGGGGCTTTGATGATGATGTCTTCAAACAAAGACAGCAGAATGATGTTGAATGGGAGTGATCCTAATGAAGAAAGAGCTCTCACTGGTGCCTGTAGGGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCTGCTGCTGCCGCAGGACAAGAGATCTCATCCTTTGGAAATCAGTTACGTCCGATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAATAGACAAGACAGCAGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAACTGGACCGCATAGAAAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTTGTATATGAAACATGGGATGCTGAATTTGCAACTGAGGCATATCGGCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGAGAAGGAAGATGAAGCAGATGAAAATCTTGATTCTCTGAGGTATGGGATGCCAAGTGATCCTAAACCCAAGCATAAGAAGAAATCGAAGAAAGCAAAATTTAAATCTCTGAAGAAACGGTCTCTAGCTTCTGAGCTGGAACCTGTTAAAGGAGAGCTGCAAGTGGAACCTATGTTCATAGATGAAGATTATCTCTCTAATGATGTGTTTTCCTATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGCAAGAAGGCTGAATCTAAACCAGCTGGTGAAGATAAGATTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCTTGAAATCAGTACTTCAGAGTTTGATACTAGTGTCTCCTCAGTGCAGCATGTTGAAGCATCAGAGTTAAAACCATGTGAGAGTGTGGTTGAATTCGAGCATAAACCAATTAGTAGATCCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCGGATTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGCTTGGTGAGTGAGACGCTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCTTTGTTGTGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTCTCCGCTCCAGACAATCCTAATTATGAAAAGGTCAACAATATAGGATCTGGAAAAGCTCTTTTAAGAGTAACAGAGGAAAATATTCGAATGTTATTAAATGTTGCTGCCGAGCAGCCCAATACAGAATATTTGATTCAGAAACACTTCATTGCCCTGCTATCCTCTGTCTGGAAGATGGCATCTCACAAAGACGGGAGACAAAATCTCCCATCTTCTGGGAATGGTGTATATTTTGGCGGAAATTTTTTCAGTTCTTCCAACCAAATTTCTCGGACTTCAGTGAAGGAAAACACAGCAACCATGAAATTTACCAATTTTGGACAGGGTGCCAGGTTGGTAGCAGCTGCACTTAATGATGCCAGCAGCAGGCGAGGGGATGAGTGTGTCATTTCTCCCAACCCTAGGAAAAAATCGTCAACCAATGCTGAACAGTTGGATATAACTCTGGAGTTTCAGGCACAAAAGGATGACTCCACAGATCCATTTCCATCTGTTATCAATTTATCAATATCTGATATAGGCCTTCCATCAGAAAATAAGGCAATGGAACCGTATCTTCTCAGAGATTCCTGTAATGACTATTGCAAAAGCACCAGTGATGCAAATGTGGCTGAGAACCGTTTCAGGAATGCAACAAGAGCTTGCGATGAAGATACTATGGGGTGGGCTTCATCGACATTCCCGACATATGATGTCAGGTCTCGATCAGTGTCAAAGTTACCTTCCTCAGGAAAGCACAAGCTTGTCTTCTCCGATCCAGTTAGACCTTCAAAATCCAAGTTCAGAAAAAATGCAGTAGAGCATGGTGAGATGCGTCACTTTCTGGCTGAGCATGTATTCCCACCCTTGCCAATAGCTGCACCGCTGAATCCCAGTCCAAGGTTCGACCTGAACCTTCCTGTCAACGAGGATATTGAGACTGGTGATCTGGAAAGCACTTCCCATTCTCAGGTGGTTGAGTGTTCATTTGAAGAAAGCTTTGGAGTTCTCCCACATGAATATGTTCCTGAATTGCTTTCAGGCCTTGATGACTGTTCATTATTGCCAGAATATGATGACATTGCCTAA

Protein Analysis

2517

Amino Acids

285.9

Weight (kDa)

5.43

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 184 2.9e-12 D-mannose binding lectin
HSA PF07529 423 - 489 4.4e-14 HSA domain
ResIII PF04851 959 - 1120 2.9e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 964 - 1249 3e-89 SNF2-related domain
DEAD PF00270 981 - 1121 9.6e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1505 - 1617 2.8e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 864, 1776, 4401
AarI CACCTGC 1 cut(s) 1058
AatII GACGTC 1 cut(s) 4605
Acc36I ACCTGC 3 cut(s) 1058, 4459, 5671
AccB1I GGYRCC 3 cut(s) 3096, 5357, 6812
AccB7I CCANNNNNTGG 2 cut(s) 5710, 6701
AccBSI CCGCTC 3 cut(s) 310, 2574, 6491
AccI GTMKAC 4 cut(s) 3514, 3607, 3852, 4465
AccII CGCG 3 cut(s) 553, 631, 880
AccIII TCCGGA 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 1277
AcyI GRCGYC 1 cut(s) 4602
AfeI AGCGCT 1 cut(s) 5028
AflII CTTAAG 1 cut(s) 3857
AflIII ACRYGT 2 cut(s) 2671, 4635
AgeI ACCGGT 2 cut(s) 2249, 3350
AhdI GACNNNNNGTC 1 cut(s) 1336
AhlI ACTAGT 1 cut(s) 6098
AjnI CCWGG 8 cut(s) 106, 589, 1089, 3614, 3832, 4299, 5097, 6815
AjuI GAANNNNNNNTTGG 6 cut(s) 2298, 2330, 3754, 3786, 7449, 7481
AleI CACNNNNGTG 1 cut(s) 1694
AloI GAACNNNNNNTCC 4 cut(s) 3311, 3343, 5894, 5926
Alw21I GWGCWC 2 cut(s) 4895, 5350
AlwNI CAGNNNCTG 2 cut(s) 2795, 5417
Ama87I CYCGRG 1 cut(s) 1901
Aor13HI TCCGGA 1 cut(s) 340
Aor51HI AGCGCT 1 cut(s) 5028
ApaI GGGCCC 1 cut(s) 3035
ArsI GACNNNNNNTTYG 4 cut(s) 2977, 3009, 6029, 6061
AseI ATTAAT 1 cut(s) 708
AsiGI ACCGGT 2 cut(s) 2249, 3350
AsiSI GCGATCGC 1 cut(s) 557
Asp700I GAANNNNTTC 6 cut(s) 1493, 4040, 6567, 6629, 7106, 7488
AspA2I CCTAGG 1 cut(s) 6887
AspLEI GCGC 7 cut(s) 633, 880, 1242, 1567, 2159, 3144, 5029
AsuC2I CCSGG 2 cut(s) 3466, 3970
AsuII TTCGAA 4 cut(s) 251, 755, 4044, 6571
AsuNHI GCTAGC 1 cut(s) 3650
AvaI CYCGRG 1 cut(s) 1901
AvrII CCTAGG 1 cut(s) 6887
AxyI CCTNAGG 3 cut(s) 4163, 4668, 7213
BaeGI GKGCMC 2 cut(s) 3035, 3101
BaeI ACNNNNGTAYC 4 cut(s) 6087, 6120, 6338, 6371
BamHI GGATCC 1 cut(s) 4655
BanI GGYRCC 3 cut(s) 3096, 5357, 6812
BanII GRGCYC 2 cut(s) 3035, 5350
BauI CACGAG 2 cut(s) 4335, 4857
BbsI GAAGAC 7 cut(s) 2981, 3223, 3645, 3894, 4053, 5283, 7225
Bbv12I GWGCWC 2 cut(s) 4895, 5350
BceAI ACGGC 4 cut(s) 779, 1292, 3531, 4091
BciT130I CCWGG 8 cut(s) 108, 591, 1091, 3616, 3834, 4301, 5099, 6817
BclI TGATCA 4 cut(s) 21, 324, 802, 4881
BcnI CCSGG 2 cut(s) 3466, 3970
BcuI ACTAGT 1 cut(s) 6098
BfmI CTRYAG 7 cut(s) 2348, 2513, 3362, 3604, 4101, 5362, 5409
BfoI RGCGCY 3 cut(s) 1243, 1568, 5030
BfrI CTTAAG 1 cut(s) 3857
BfuAI ACCTGC 3 cut(s) 1058, 4459, 5671
BglII AGATCT 2 cut(s) 658, 5436
BlnI CCTAGG 1 cut(s) 6887
BlpI GCTNAGC 1 cut(s) 7314
BmcAI AGTACT 1 cut(s) 6082
BmeRI GACNNNNNGTC 1 cut(s) 1336
BmeT110I CYCGRG 1 cut(s) 1901
BmrI ACTGGG 1 cut(s) 7353
BmtI GCTAGC 1 cut(s) 3654
BmuI ACTGGG 1 cut(s) 7353
BpiI GAAGAC 7 cut(s) 2981, 3223, 3645, 3894, 4053, 5283, 7225
BplI GAGNNNNNCTC 4 cut(s) 102, 134, 1595, 1627
BpmI CTGGAG 3 cut(s) 2442, 6477, 6953
Bpu10I CCTNAGC 3 cut(s) 101, 1362, 4538
Bpu1102I GCTNAGC 1 cut(s) 7314
Bpu14I TTCGAA 4 cut(s) 251, 755, 4044, 6571
BpuEI CTTGAG 5 cut(s) 112, 1601, 4147, 4812, 5520
BpuMI CCSGG 2 cut(s) 3466, 3970
Bsa29I ATCGAT 1 cut(s) 4876
BsaAI YACGTR 1 cut(s) 4638
BsaBI GATNNNNATC 2 cut(s) 2484, 5760
BsaHI GRCGYC 1 cut(s) 4602
BsaI GGTCTC 4 cut(s) 145, 3393, 5862, 7191
BsaWI WCCGGW 6 cut(s) 299, 340, 2249, 3350, 4842, 6301
Bse118I RCCGGY 2 cut(s) 2249, 3350
Bse21I CCTNAGG 3 cut(s) 4163, 4668, 7213
Bse3DI GCAATG 4 cut(s) 1661, 6633, 7035, 7545
Bse8I GATNNNNATC 2 cut(s) 2484, 5760
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 8 cut(s) 108, 591, 1091, 3616, 3834, 4301, 5099, 6817
BseCI ATCGAT 1 cut(s) 4876
BseJI GATNNNNATC 2 cut(s) 2484, 5760
BseMI GCAATG 4 cut(s) 1661, 6633, 7035, 7545
BseRI GAGGAG 8 cut(s) 2471, 2474, 3348, 4085, 4231, 4685, 5564, 6097
BseSI GKGCMC 2 cut(s) 3035, 3101
Bsh1236I CGCG 3 cut(s) 553, 631, 880
Bsh1285I CGRYCG 2 cut(s) 557, 2254
BshNI GGYRCC 3 cut(s) 3096, 5357, 6812
BshTI ACCGGT 2 cut(s) 2249, 3350
BshVI ATCGAT 1 cut(s) 4876
BsiEI CGRYCG 2 cut(s) 557, 2254
BsiHKAI GWGCWC 2 cut(s) 4895, 5350
BsiHKCI CYCGRG 1 cut(s) 1901
BslFI GGGAC 6 cut(s) 107, 1176, 1307, 3035, 4979, 5516
BsmBI CGTCTC 3 cut(s) 645, 919, 6377
BsmFI GGGAC 6 cut(s) 107, 1176, 1307, 3035, 4979, 5516
BsmI GAATGC 2 cut(s) 12, 7120
Bso31I GGTCTC 4 cut(s) 145, 3393, 5862, 7191
BsoBI CYCGRG 1 cut(s) 1901
Bsp119I TTCGAA 4 cut(s) 251, 755, 4044, 6571
Bsp120I GGGCCC 1 cut(s) 3031
Bsp1286I GDGCHC 4 cut(s) 3035, 3101, 4895, 5350
Bsp13I TCCGGA 1 cut(s) 340
Bsp1407I TGTACA 2 cut(s) 228, 540
Bsp1720I GCTNAGC 1 cut(s) 7314
Bsp19I CCATGG 3 cut(s) 1188, 2493, 5001
Bsp68I TCGCGA 1 cut(s) 553
BspDI ATCGAT 1 cut(s) 4876
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 3 cut(s) 553, 631, 880
BspHI TCATGA 2 cut(s) 2125, 2982
BspMAI CTGCAG 4 cut(s) 2352, 2517, 4105, 5413
BspMI ACCTGC 3 cut(s) 1058, 4459, 5671
BspOI GCTAGC 1 cut(s) 3654
BspQI GCTCTTC 1 cut(s) 1437
BspT104I TTCGAA 4 cut(s) 251, 755, 4044, 6571
BspT107I GGYRCC 3 cut(s) 3096, 5357, 6812
BspTI CTTAAG 1 cut(s) 3857
BspTNI GGTCTC 4 cut(s) 145, 3393, 5862, 7191
BsrBI CCGCTC 3 cut(s) 310, 2574, 6491
BsrDI GCAATG 4 cut(s) 1661, 6633, 7035, 7545
BsrFI RCCGGY 2 cut(s) 2249, 3350
BsrGI TGTACA 2 cut(s) 228, 540
BssAI RCCGGY 2 cut(s) 2249, 3350
BssNI GRCGYC 1 cut(s) 4602
BssSI CACGAG 2 cut(s) 4335, 4857
BssT1I CCWWGG 8 cut(s) 267, 1188, 1205, 2493, 5001, 5733, 6887, 7364
Bst2BI CACGAG 2 cut(s) 4335, 4857
Bst2UI CCWGG 8 cut(s) 108, 591, 1091, 3616, 3834, 4301, 5099, 6817
Bst6I CTCTTC 5 cut(s) 762, 1302, 1437, 2886, 3146
BstACI GRCGYC 1 cut(s) 4602
BstAFI CTTAAG 1 cut(s) 3857
BstAPI GCANNNNNTGC 5 cut(s) 1038, 2795, 4109, 4340, 5417
BstAUI TGTACA 2 cut(s) 228, 540
BstBAI YACGTR 1 cut(s) 4638
BstBI TTCGAA 4 cut(s) 251, 755, 4044, 6571
BstDSI CCRYGG 4 cut(s) 747, 1188, 2493, 5001
BstENI CCTNNNNNAGG 3 cut(s) 398, 5883, 7212
BstFNI CGCG 3 cut(s) 553, 631, 880
BstH2I RGCGCY 3 cut(s) 1243, 1568, 5030
BstHHI GCGC 7 cut(s) 633, 880, 1242, 1567, 2159, 3144, 5029
BstMCI CGRYCG 2 cut(s) 557, 2254
BstNI CCWGG 8 cut(s) 108, 591, 1091, 3616, 3834, 4301, 5099, 6817
BstSFI CTRYAG 7 cut(s) 2348, 2513, 3362, 3604, 4101, 5362, 5409
BstSLI GKGCMC 2 cut(s) 3035, 3101
BstUI CGCG 3 cut(s) 553, 631, 880
BstV2I GAAGAC 7 cut(s) 2981, 3223, 3645, 3894, 4053, 5283, 7225
BstX2I RGATCY 7 cut(s) 658, 4168, 4655, 5436, 6185, 6530, 6964
BstXI CCANNNNNNTGG 2 cut(s) 361, 4847
BstYI RGATCY 7 cut(s) 658, 4168, 4655, 5436, 6185, 6530, 6964
Bsu15I ATCGAT 1 cut(s) 4876
Bsu36I CCTNAGG 3 cut(s) 4163, 4668, 7213
BsuTUI ATCGAT 1 cut(s) 4876
BtgI CCRYGG 4 cut(s) 747, 1188, 2493, 5001
BtgZI GCGATG 1 cut(s) 7146
BtsI GCAGTG 1 cut(s) 6213
BtuMI TCGCGA 1 cut(s) 553
BveI ACCTGC 3 cut(s) 1058, 4459, 5671
CaiI CAGNNNCTG 2 cut(s) 2795, 5417
CciI TCATGA 2 cut(s) 2125, 2982
CfoI GCGC 7 cut(s) 633, 880, 1242, 1567, 2159, 3144, 5029
Cfr10I RCCGGY 2 cut(s) 2249, 3350
ClaI ATCGAT 1 cut(s) 4876
CseI GACGC 3 cut(s) 3345, 6394, 7289
CsiI ACCWGGT 1 cut(s) 4299
CspAI ACCGGT 2 cut(s) 2249, 3350
DraI TTTAAA 2 cut(s) 4000, 5839
DriI GACNNNNNGTC 1 cut(s) 1336
EaeI YGGCCR 1 cut(s) 1277
Eam1104I CTCTTC 5 cut(s) 762, 1302, 1437, 2886, 3146
Eam1105I GACNNNNNGTC 1 cut(s) 1336
EarI CTCTTC 5 cut(s) 762, 1302, 1437, 2886, 3146
EciI GGCGGA 4 cut(s) 3518, 3533, 4653, 6738
Ecl136II GAGCTC 1 cut(s) 5348
Eco130I CCWWGG 8 cut(s) 267, 1188, 1205, 2493, 5001, 5733, 6887, 7364
Eco147I AGGCCT 3 cut(s) 936, 7008, 7509
Eco24I GRGCYC 2 cut(s) 3035, 5350
Eco31I GGTCTC 4 cut(s) 145, 3393, 5862, 7191
Eco32I GATATC 1 cut(s) 6428
Eco47III AGCGCT 1 cut(s) 5028
Eco53kI GAGCTC 1 cut(s) 5348
Eco81I CCTNAGG 3 cut(s) 4163, 4668, 7213
Eco88I CYCGRG 1 cut(s) 1901
EcoICRI GAGCTC 1 cut(s) 5348
EcoNI CCTNNNNNAGG 3 cut(s) 398, 5883, 7212
EcoO109I RGGNCCY 5 cut(s) 599, 1249, 3031, 3032, 5503
EcoRI GAATTC 4 cut(s) 1111, 4120, 4979, 6161
EcoRII CCWGG 8 cut(s) 106, 589, 1089, 3614, 3832, 4299, 5097, 6815
EcoRV GATATC 1 cut(s) 6428
EcoT14I CCWWGG 8 cut(s) 267, 1188, 1205, 2493, 5001, 5733, 6887, 7364
EcoT22I ATGCAT 2 cut(s) 3399, 4029
EcoT38I GRGCYC 2 cut(s) 3035, 5350
ErhI CCWWGG 8 cut(s) 267, 1188, 1205, 2493, 5001, 5733, 6887, 7364
Esp3I CGTCTC 3 cut(s) 645, 919, 6377
FaqI GGGAC 6 cut(s) 107, 1176, 1307, 3035, 4979, 5516
FauNDI CATATG 4 cut(s) 1037, 2374, 3026, 7174
FbaI TGATCA 4 cut(s) 21, 324, 802, 4881
FblI GTMKAC 4 cut(s) 3514, 3607, 3852, 4465
FriOI GRGCYC 2 cut(s) 3035, 5350
GlaI GCGC 7 cut(s) 632, 879, 1241, 1566, 2158, 3143, 5028
GsuI CTGGAG 3 cut(s) 2442, 6477, 6953
HaeII RGCGCY 3 cut(s) 1243, 1568, 5030
HgaI GACGC 3 cut(s) 3345, 6394, 7289
HhaI GCGC 7 cut(s) 633, 880, 1242, 1567, 2159, 3144, 5029
Hin1I GRCGYC 1 cut(s) 4602
Hin6I GCGC 7 cut(s) 631, 878, 1240, 1565, 2157, 3142, 5027
HinP1I GCGC 7 cut(s) 631, 878, 1240, 1565, 2157, 3142, 5027
HincII GTYRAC 7 cut(s) 1939, 2695, 3348, 4734, 6520, 6903, 7390
HindII GTYRAC 7 cut(s) 1939, 2695, 3348, 4734, 6520, 6903, 7390
HindIII AAGCTT 7 cut(s) 130, 466, 4247, 4309, 4991, 7226, 7462
HpaI GTTAAC 2 cut(s) 1939, 3348
Hpy99I CGWCG 4 cut(s) 553, 1278, 2259, 3339
HpyCH4IV ACGT 5 cut(s) 161, 321, 4602, 4637, 5461
HpySE526I ACGT 5 cut(s) 161, 321, 4602, 4637, 5461
Hsp92I GRCGYC 1 cut(s) 4602
HspAI GCGC 7 cut(s) 631, 878, 1240, 1565, 2157, 3142, 5027
KflI GGGWCCC 1 cut(s) 5503
Kpn2I TCCGGA 1 cut(s) 340
Ksp22I TGATCA 4 cut(s) 21, 324, 802, 4881
KspAI GTTAAC 2 cut(s) 1939, 3348
LguI GCTCTTC 1 cut(s) 1437
LmnI GCTCC 5 cut(s) 307, 1540, 5005, 6369, 6496
MabI ACCWGGT 1 cut(s) 4299
MaeII ACGT 5 cut(s) 161, 321, 4602, 4637, 5461
MbiI CCGCTC 3 cut(s) 310, 2574, 6491
MfeI CAATTG 6 cut(s) 635, 999, 3070, 3762, 4293, 4455
MflI RGATCY 7 cut(s) 658, 4168, 4655, 5436, 6185, 6530, 6964
MhlI GDGCHC 4 cut(s) 3035, 3101, 4895, 5350
MlyI GAGTC 5 cut(s) 1346, 2690, 4930, 5689, 6950
MmeI TCCRAC 7 cut(s) 354, 648, 1320, 2245, 2753, 6768, 6900
Mph1103I ATGCAT 2 cut(s) 3399, 4029
MroI TCCGGA 1 cut(s) 340
MroXI GAANNNNTTC 6 cut(s) 1493, 4040, 6567, 6629, 7106, 7488
MspCI CTTAAG 1 cut(s) 3857
MunI CAATTG 6 cut(s) 635, 999, 3070, 3762, 4293, 4455
Mva1269I GAATGC 2 cut(s) 12, 7120
MvaI CCWGG 8 cut(s) 108, 591, 1091, 3616, 3834, 4301, 5099, 6817
MvnI CGCG 3 cut(s) 553, 631, 880
NciI CCSGG 2 cut(s) 3466, 3970
NcoI CCATGG 3 cut(s) 1188, 2493, 5001
NdeI CATATG 4 cut(s) 1037, 2374, 3026, 7174
NheI GCTAGC 1 cut(s) 3650
NmeAIII GCCGAG 1 cut(s) 6619
NmuCI GTSAC 6 cut(s) 887, 2402, 4437, 4559, 4796, 7301
NruI TCGCGA 1 cut(s) 553
NsiI ATGCAT 2 cut(s) 3399, 4029
NspV TTCGAA 4 cut(s) 251, 755, 4044, 6571
OliI CACNNNNGTG 1 cut(s) 1694
PacI TTAATTAA 1 cut(s) 3954
PaeI GCATGC 2 cut(s) 1402, 5120
PaeR7I CTCGAG 1 cut(s) 1901
PagI TCATGA 2 cut(s) 2125, 2982
PaqCI CACCTGC 1 cut(s) 1058
PceI AGGCCT 3 cut(s) 936, 7008, 7509
PciI ACATGT 1 cut(s) 2671
PciSI GCTCTTC 1 cut(s) 1437
PcsI WCGNNNNNNNCGW 1 cut(s) 7166
PctI GAATGC 2 cut(s) 12, 7120
PdmI GAANNNNTTC 6 cut(s) 1493, 4040, 6567, 6629, 7106, 7488
PflMI CCANNNNNTGG 2 cut(s) 5710, 6701
PfoI TCCNGGA 3 cut(s) 1089, 3464, 3614
PinAI ACCGGT 2 cut(s) 2249, 3350
Ple19I CGATCG 1 cut(s) 557
PleI GAGTC 5 cut(s) 1345, 2690, 4930, 5689, 6950
PpsI GAGTC 5 cut(s) 1345, 2690, 4930, 5689, 6950
Ppu21I YACGTR 1 cut(s) 4638
PpuMI RGGWCCY 1 cut(s) 5503
PscI ACATGT 1 cut(s) 2671
PshBI ATTAAT 1 cut(s) 708
PsiI TTATAA 3 cut(s) 864, 1776, 4401
Psp124BI GAGCTC 1 cut(s) 5350
Psp5II RGGWCCY 1 cut(s) 5503
Psp6I CCWGG 8 cut(s) 106, 589, 1089, 3614, 3832, 4299, 5097, 6815
PspGI CCWGG 8 cut(s) 106, 589, 1089, 3614, 3832, 4299, 5097, 6815
PspOMI GGGCCC 1 cut(s) 3031
PspPPI RGGWCCY 1 cut(s) 5503
PsrI GAACNNNNNNTAC 2 cut(s) 6219, 6251
PstI CTGCAG 4 cut(s) 2352, 2517, 4105, 5413
PstNI CAGNNNCTG 2 cut(s) 2795, 5417
PsuI RGATCY 7 cut(s) 658, 4168, 4655, 5436, 6185, 6530, 6964
PvuI CGATCG 1 cut(s) 557
PvuII CAGCTG 7 cut(s) 1694, 2792, 3574, 4331, 5414, 6017, 6830
RgaI GCGATCGC 1 cut(s) 557
RruI TCGCGA 1 cut(s) 553
SacI GAGCTC 1 cut(s) 5350
SapI GCTCTTC 1 cut(s) 1437
ScaI AGTACT 1 cut(s) 6082
SchI GAGTC 5 cut(s) 1346, 2690, 4930, 5689, 6950
SduI GDGCHC 4 cut(s) 3035, 3101, 4895, 5350
SexAI ACCWGGT 1 cut(s) 4299
SfaAI GCGATCGC 1 cut(s) 557
SfcI CTRYAG 7 cut(s) 2348, 2513, 3362, 3604, 4101, 5362, 5409
Sfr274I CTCGAG 1 cut(s) 1901
SfuI TTCGAA 4 cut(s) 251, 755, 4044, 6571
SgfI GCGATCGC 1 cut(s) 557
SlaI CTCGAG 1 cut(s) 1901
SmiI ATTTAAAT 1 cut(s) 5839
SmlI CTYRAG 7 cut(s) 127, 1580, 1901, 3857, 4126, 4827, 5535
SmoI CTYRAG 7 cut(s) 127, 1580, 1901, 3857, 4126, 4827, 5535
SpeI ACTAGT 1 cut(s) 6098
SphI GCATGC 2 cut(s) 1402, 5120
SseBI AGGCCT 3 cut(s) 936, 7008, 7509
SspI AATATT 4 cut(s) 1474, 2332, 6568, 6614
SstI GAGCTC 1 cut(s) 5350
StuI AGGCCT 3 cut(s) 936, 7008, 7509
StyI CCWWGG 8 cut(s) 267, 1188, 1205, 2493, 5001, 5733, 6887, 7364
SwaI ATTTAAAT 1 cut(s) 5839
TaiI ACGT 5 cut(s) 164, 324, 4605, 4640, 5464
TatI WGTACW 5 cut(s) 228, 540, 4236, 5583, 6080
TauI GCSGC 1 cut(s) 5426
TseFI GTSAC 6 cut(s) 887, 2402, 4437, 4559, 4796, 7301
Tsp45I GTSAC 6 cut(s) 887, 2402, 4437, 4559, 4796, 7301
TspGWI ACGGA 7 cut(s) 250, 669, 943, 1465, 2648, 2837, 4451
Van91I CCANNNNNTGG 2 cut(s) 5710, 6701
Vha464I CTTAAG 1 cut(s) 3857
VspI ATTAAT 1 cut(s) 708
XagI CCTNNNNNAGG 3 cut(s) 398, 5883, 7212
XbaI TCTAGA 4 cut(s) 2647, 3886, 5491, 6281
XcmI CCANNNNNNNNNTGG 4 cut(s) 1287, 2491, 4353, 5209
XhoI CTCGAG 1 cut(s) 1901
XmaJI CCTAGG 1 cut(s) 6887
XmiI GTMKAC 4 cut(s) 3514, 3607, 3852, 4465
XmnI GAANNNNTTC 6 cut(s) 1493, 4040, 6567, 6629, 7106, 7488
ZraI GACGTC 1 cut(s) 4603
ZrmI AGTACT 1 cut(s) 6082
Zsp2I ATGCAT 2 cut(s) 3399, 4029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.