MD10G1206600.v1.1
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014066
Physical Location & Seq
Reverse (-)
30546885 .. 30560114
13230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1206600.v1.1.491

Sequence Viewer

Length: 6486 bp
ATGGCTTCCAAAGGTCCAAGGTCCAAGCTTGATCATGAACCCAGACCTAAGCGCCAAAAGGCCCTTGAAACTGCAAGTGAACCTCGTCGGCCAAAAGCTCACTGGGATCATGTTTTAGAAGAGATGATATGGCTTTCAAAGGATTTCGAGTCAGAGAGAAAATGGAAATTAGCTCAGGCGAAGAAAGTTGCTTTAAGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAGGAAATTGAAGGAGGAAGAGCAACGGCTAAAGAAGATTGCAGTCAATATATCGAAGGATGTAAAGAAATTCTGGCTGAAAATAGAAAAGCTGGTGCTTTACAAGCATCAGATGGAACTTGATGAGAAAAAGAAAAAGGCACTTGATAAGCAGCTTGAGTTCCTTCTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGGGGACAGCTACAAGCCTGTTCAGCAATATCCTACACAAGGTCAACCAAGTATCCAATTCAAAGAGATGGACAAAAATGATACTAACAAATCAACCGAATTGAATGCCGAACCTGACACTGAAAGAGCCGACATTGACGTTGATGATGATGATTATGACGTACAGTCTGAAGATAGAACTGAGGATGATGATGAGAACACTATTGAGGAAGATGAGGCTCTTTTTACTGAACAAGAAAGGAAGGAAGAGTTGGCTGCTTTACAGAATGAAGTAGATCTTCCACTTGGGGAGCTACTCAAACAATATCCTATGGAGAAAGATGGACTTTCAGTTAGCCCAGAAAGTAGTACAGAAAAGGGTGAAGATGCTACAGAAATGACATCAGGAGACGATGGAGCAGAAATGACCTCAATAGAGGAAGAGCATGGTATGTCTCCTGGTAAGAATGAGGATGGTGCAGAACTGATCTTGTTGGGGAAAAAGGATGATATGTCTCCAGGGAAGGATGAAGATGGCAAAGAAATGACTTCGGTGGAGGAAGAGCGTGTTAAACAGGATGAAGATGGCACAAAAATGACCTCAGTTGGAGAAGAGAAGGGTATGTGTTCAGAAAAGGTTGAAGATGCCGCAGAAATAACCTCAGTGGGGGAGGAACCTGGTGAAAAAAGTGATATTTTTGTGTCCGATATAACCGAGAAAATCAACTCAGATGTTGTCACTGGTCGTCGTTGTGTGCAAAGTAATGGCGGTTTGTCCATTTCAGAAAGCCATTTATCAGAGATTGAGATAAATGAAGATAAAAATCATTCAGAAGCGCCTGTACAGCTTGCCAAAGGACAGATGCGATATGATTTTAATGATGAACATGAAGATGGTGACTTCATTCTTGCTGCTGGAGAAGAACAGGATGATGAGACAACCTTATCAGAGGAGGAGGAACTGGAAAAAGCAGATACCAATGACCGTATGGATGAGATTGCCTTACTGCAGAAGGAGAGTGAAGTTCCTTTACAGGAACTGCTTGCAAGGTATAAAAAGGATTTAAACAGTGATGAAGTTGCAGATGGTGAATCTGAATATGGCTCTGCTCTATCAGAGGATTTTGTGGATTCTTCATCTCTTGAAGACCTTGAACCAAAGCAAGATGATTATATGGATGAAGATATTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCTACTGAAGAACACAGTGCAAGTATAACTAAGATTTCAGAAGGGGGAAAAGACAGTGAAAACAGACTTGAGGATGCGGCTGCTGCTGCTAGATCTGCACAGCCAACTGGAAACACCTTCTCCACAACCAATGTCCGCACAAAGTTCCCCTTTCTTCTTAAGTTCCCTCTACGTGAGTATCAGCATATTGGCTTGGATTGGCTTGTAACGATGTATGAGAAAAGACTGAATGGGATTCTAGCCGACGAGATGGGGCTTGGGAAGACAATCATGACAATTGCTTTGCTTGCACACCTAGCATGTGAAAAGGGAATATGGGGTCCTCATCTTATTGTGGTCCCAACTAGTGTAATGCTTAATTGGGAGACTGAGTTTCTTAAATGGTGTCCAGCTTTCAAAATCCTAACTTATTTTGGAAGTGCTAAAGAGCGCAAGTTGAAAAGGCAAGGCTGGTTGAAACCCAACTCATTTCATATATGCATAACGACATACAGACTGGTTATTCAAGATTCAAAAGTTTTCAAGCGGAAGAAGTGGAAGTACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCTCAGAGGTGGCAGACTCTTTTGAACTTCAATTCCAAACGGCGCATTTTGTTAACTGGTACTCCTCTGCAAAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCATGTATTTCAGTCTCATCAGGAATTCAAGGATTGGTTCTCTAATCCAATATCAGGGATGGTTGAGGGGCAAGAAAGAGTAAATAAAGAAGTTCTTGATCGTCTGCATAATGTTCTTCGTCCATTCATTCTCCGTCGTTTAAAACGAGATGTGGAGAAGCAGCTCCCTATGAAACATGAGCATGTCATATACTGTAGACTCTCCAAGAGGCAGCGTAACTTGTATGAAGACTTCATTGCTAGCTCCGAAACACAAGCAACTCTTGCAAGTGCCAATTTTTTTGGAATGATAAGTATTATAATGCAACTTCGTAAAGTATGCAATCATCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTATGATATGGGGGGTATTTACACACAATTGAGTTCTTCAGTATGTTCAATTCTTTTACCTGGACCCTTTTCTGCGGTAGACCTTAAGGGTTTGGGGTTTTTATTTACTCATTTAGATTTTACAATGAATTCTTGGGAGAGTGATGAAGTTAAAGCTCTTGCTACGCCATCAAATTCAATCAAGGAGCGTGTTGAACTGATTAATCTTGAAGATATTGGTGGACTTAAACATCGCAAGAAGTTGCATGGTACCAATATTTTTGAAGAGATTCATAAAGCAATTATGGAGGAGAGACTAAGACAAGCAAAGGAACATGCTGCAGCTGTTGCATGGTGGAATTCCTTGAGGTGCGACAGGAAACCCATATACTCAACAAGCCTAAGGGAGCTTGTTACAGTAAGACATCCTGTTTTTGATATTCATTGTCACAAGGCTAATCCTATATCCTACATGCACTCCTCAAAGCTTGCTGATATAGTCCTTTCACCAGTGGAGCGCTTCCAGAAAACGATTGACCTGGTTGAAAGCTTCTTGTTTGCAATCCCTGCTGCGCGAGCGCCACCACCTGTATGTTGGTGCAATAAGTCTGTAAGTTCTGTGTTTCTGCATCCAGCTTATAAGCAGAAATGCTCTGAATACTTGTCTCCACTTTTATCACCGTTCAGACCTGCTATTGTTCGAAGGCAAGTATATTTTCCTGACAGGCGACTTATACAATTTGACTGTGGTAAATTGCAAGAGCTTGCAGTTTTACTTAGAAAACTAAAGTCAGAAGGTCACAGAGCACTAATATTCACTCAGATGACCAAGATGCTTGATACCTTGGAGGCTTTCATCAATCTGTATGGTTACACATATATGCGTTTAGATGGATCCACTCCGCCCGAGGAGAGGCAGACTTTAATGCAGCGGTTTAACACGAATCCAAAAATTTTTCTTTTCATATTGTCAACCCGTAGTGGGGGTGTGGGTATTAACCTAGTTGGGGCAGACACTGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGATGCCACCGTATTGGACAGACACGTGAAGTAAATATTTATCGGTTGATCAGTCAGAGTACCATTGAAGAAAATATCCTGAAGAAGGCAAATCAGAAGCGTGCACTTGATGATCTAGTTATACAGAGTGGGGGCTACAACACTGAATTCTTCAAAAAGCTTGACCCAATGGAGCTTTTCTCTGGTCTTAGGGCACTTCCCGTGAAGAATATGCAAAAGGAGAAAAATCACAACACAACTGAGGTTTCTCTGTCTAATGCTGATCTTGAAGCTGCTCTAAAGCATGCAGAAGATGAAGCCGATTACATGGCATTAAAGAAAGTTGAGCAGGAAGAAGCTGTGGACAACCAAGAATTTACAGAAGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTAAATGAGGACGATGTGAAGATTGATGAACCTGCAGATCGGGGTGGATGCGTGGCATCTTCAAGCAAAGAAAATGGGGTTGATCCTAATGAAGGAAGAGCTCATACTGTTGCTTGTAGAGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCGGCTGCTGCAGCTGCAGGACAAGAAATCTCATCATTTGGCGATCAATTACGTCCAATAGATAGGTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATTATAGACAAGAAAGCAGTGGAATCTCAGGCTAGGTTTGAGGAGACAGAATGGGAACTGGACCGCATTGAAAAATACAAGGAAGAAATGGAAGCTGAGATTGATGAAGATGAGGAACCTCTTGTATATGAAACATGGGATGCTGATTTTGCAACTGAGGCATATCGGCAGCAGGTTGAGGCTTTAGCTCAAATTCAGTTGGAGGAACTGGAATACGAAGCTAAACTGAAGGAAGATGAAGCAGAGGAAAATTGTGATTCTATGAAGAATGAGATGCCAATTGATCCTAAACCAAAGACTAAGAAGAAATCGAAGAAAGCCAAATTTAAATCTCTGAAGAAGCAGTCTCTAGCTTCTGAACTGAAACCTTTGAAAGGAGAGCTACAAGCCGAACCTATATCCATAGATGAAGACTCTCTCTCTCATGAGATGGTTACCTATTCCGATACAGAATCACCACGTTCAAGTGTGCAGAGAAAACGTAAGAAGGCTGAAGTCATACCAGTTGGCGAAGAGAAGAGTTCAAAGAAGAAGTCTAAGAAGCTCAAGAAGTCTACTCTTGAAATATGTCCTACGGAGTTTGATACTAACCTCTCTACTATGGATCATGATGAAGTTACAGAGTCAAAACCATGTGAGAGTATGGTTGAGTTTGATCATAAGCCAGCTAGCAGAAGCAAAATGGGAGGGAAAATTTCTATCACTTCCATGCCTGTAAAGCGAATTCTACTGATTAAACCAGAGAAGCTAAAGAAGGGGAATATTTGGTCTAGAGACTGTATTCCATCACCAGATTTTTGGTTGTCACAGGAGGATGCTATGTTATGTGCTGTTGTACATGAGTATGGTCCTCATTGGAGCTTGGTTAGTGACGTGCTTTATGGAATGACCACTGGTGGGTTTTATAGGGGAAGATATCGCCATCCCATCCATTGCTGTGAGAGATTTAGGGAACTCATCCAAAGATATGTTTTGTCTATACCGGACAATCCTAACTATGAAAAAGTTAACAATATAGGATCTGGAAAGGCTCTTCTAAAAGTGACAGAGGATAATATTCGAATGTTATTAAATGTTGCCGCCGACCAGCCCAATACAGAGTTCCTCATTCAGAAACACTTCACTGCCTTGCTTTCAGCTGTCTGGAAGGTGACATCCCACAAAGACCGCCGAAAAAATCTCCCATCTTCCCAGAATGGTTTATATTTTGGGGGAAGCATTTTCAGCCCTTCCAACCAAAAGTCTCAGACTCCCATGATGGAACGTACAGAAAGAATGAAATTGACCAATTTCGGACACAGTACCAAGTTGGTAGCGGCTGCACTGAATGATGCCAGCAACAGACAGGGTACCAAGTTGGTACCAGCTGCACTTAATGATGCCAGCAGCAGGTGTGACAATGACACAGTGTTTCGTCCAAACCTGGGGAAAGATTCTTCAATTGAATCAGAACGGTTGGATATAACTCTGGAATTTCAGGGAGCAAAAGATGACACAATGGATGAATTGCCATCTGTTTTAAATTTATCAATATCTGATTCTGACCGTTTTCCATTGCTAAATAAAGCTACTGAAGATCATCATCTAAGGAAATCAAGCAATGTAAATTTGGCTGAGAACCGTTTCAGGACCGCAACAAGAACTTGCAATGAAGATGCCATGGGATGGGCTTCATCGGTATTCCCAACAAATGATCTCAGGTCTCGGTCAATGTCAAAGTTACCGACCACGGGAAAGCACAAACTTGTCTTCTCTGATTCCGTTAGACCTTCCAAATCGAAGATCAGAAAAAGTTCAGTGGAGCATGGTGAGATGCGTAACTTCGTTTCTGAGCAGGTACTCCCCCCTTTCCCAATGGCTGCACCACTGAATCCAAATCCGAGGTTTGACCTGAACACCCCCATCGATGAGGATGCTGGAATTTATGAATTGGAAAGCAATTCCTTTTCTTGCCTTGACGAGTCTTTGCTGGAAATGGAAACCTTCGGAGTTCTCCCACATGAATACGTTCCTGGCTTGATTGGAGGCCTCGATGATGAACAATTGCCAGAATATACCGACATTGGGTAA

Protein Analysis

2162

Amino Acids

245.97

Weight (kDa)

5.16

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 93 1.4e-15 HSA domain
ResIII PF04851 604 - 765 1.4e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 609 - 894 4e-89 SNF2-related domain
DEAD PF00270 626 - 766 3.9e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1147 - 1259 2.2e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2645, 3327
AarI CACCTGC 1 cut(s) 5796
Acc36I ACCTGC 5 cut(s) 3385, 4195, 4579, 5796, 6241
Acc65I GGTACC 3 cut(s) 2957, 5765, 5776
AccB1I GGYRCC 3 cut(s) 2957, 5765, 5776
AccB7I CCANNNNNTGG 1 cut(s) 6081
AccI GTMKAC 3 cut(s) 2542, 2787, 4970
AccII CGCG 1 cut(s) 3262
AclWI GGATC 7 cut(s) 114, 3576, 3589, 4232, 4694, 5028, 5443
AcoI YGGCCR 1 cut(s) 89
AcuI CTGAAG 8 cut(s) 636, 1674, 2730, 3860, 4664, 4772, 4929, 6009
AcvI CACGTG 1 cut(s) 3785
AfeI AGCGCT 1 cut(s) 3206
AflII CTTAAG 2 cut(s) 1805, 2792
AflIII ACRYGT 1 cut(s) 3782
AhdI GACNNNNNGTC 1 cut(s) 610
AhlI ACTAGT 1 cut(s) 1991
AjiI CACGTC 1 cut(s) 5290
AjnI CCWGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
AjuI GAANNNNNNNTTGG 6 cut(s) 680, 712, 2689, 2721, 5501, 5533
AloI GAACNNNNNNTCC 2 cut(s) 2246, 2278
Alw21I GWGCWC 3 cut(s) 3496, 3865, 4258
Alw44I GTGCAC 1 cut(s) 3861
AlwI GGATC 7 cut(s) 114, 3576, 3589, 4232, 4694, 5028, 5443
AlwNI CAGNNNCTG 2 cut(s) 1465, 3704
Ama87I CYCGRG 1 cut(s) 3593
Aor51HI AGCGCT 1 cut(s) 3206
AoxI GGCC 3 cut(s) 60, 89, 6442
ApaLI GTGCAC 1 cut(s) 3861
ArsI GACNNNNNNTTYG 4 cut(s) 1912, 1944, 4934, 4966
AseI ATTAAT 1 cut(s) 2910
Asp700I GAANNNNTTC 5 cut(s) 2976, 5534, 6038, 6208, 6423
Asp718I GGTACC 3 cut(s) 2957, 5765, 5776
AspLEI GCGC 7 cut(s) 54, 1264, 2079, 2274, 3207, 3262, 3268
AsuII TTCGAA 2 cut(s) 3388, 5476
AsuNHI GCTAGC 2 cut(s) 2585, 5084
AvaI CYCGRG 1 cut(s) 3593
AxyI CCTNAGG 1 cut(s) 3089
BaeGI GKGCMC 2 cut(s) 3865, 3955
BaeI ACNNNNGTAYC 2 cut(s) 1808, 1841
BamHI GGATCC 1 cut(s) 3581
BanI GGYRCC 3 cut(s) 2957, 5765, 5776
BanII GRGCYC 1 cut(s) 4258
BbrPI CACGTG 1 cut(s) 3785
BbsI GAAGAC 5 cut(s) 1578, 1916, 2580, 4833, 6157
Bbv12I GWGCWC 3 cut(s) 3496, 3865, 4258
BceAI ACGGC 2 cut(s) 278, 2285
BcgI CGANNNNNNTGC 4 cut(s) 533, 567, 6311, 6345
BciT130I CCWGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BciVI GTATCC 1 cut(s) 509
BclI TGATCA 3 cut(s) 31, 3807, 5071
BcuI ACTAGT 1 cut(s) 1991
BfmI CTRYAG 7 cut(s) 816, 1433, 2539, 3027, 4188, 4326, 4332
BfoI RGCGCY 4 cut(s) 55, 1265, 3208, 3269
BfrI CTTAAG 2 cut(s) 1805, 2792
BfuAI ACCTGC 5 cut(s) 3385, 4195, 4579, 5796, 6241
BfuI GTATCC 1 cut(s) 509
BglII AGATCT 2 cut(s) 721, 1739
BmcAI AGTACT 1 cut(s) 2189
Bme1390I CCNGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BmeRI GACNNNNNGTC 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 3593
BmgBI CACGTC 1 cut(s) 5290
BmrFI CCNGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BmrI ACTGGG 1 cut(s) 112
BmtI GCTAGC 2 cut(s) 2589, 5088
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 5 cut(s) 1578, 1916, 2580, 4833, 6157
BplI GAGNNNNNCTC 4 cut(s) 407, 439, 3923, 3955
BpmI CTGGAG 2 cut(s) 927, 1362
Bpu10I CCTNAGC 2 cut(s) 48, 174
Bpu14I TTCGAA 2 cut(s) 3388, 5476
BpuEI CTTGAG 5 cut(s) 413, 1736, 3073, 3738, 4946
Bsa29I ATCGAT 2 cut(s) 3760, 6321
BsaAI YACGTR 2 cut(s) 1820, 3785
BsaBI GATNNNNATC 2 cut(s) 1248, 5997
BsaI GGTCTC 2 cut(s) 2328, 6123
BsaJI CCNNGG 8 cut(s) 17, 944, 3531, 3594, 5839, 6075, 6144, 6296
BsaWI WCCGGW 1 cut(s) 5398
BsaXI ACNNNNNCTCC 8 cut(s) 1751, 1781, 2003, 2033, 2275, 2305, 3149, 3179
Bse21I CCTNAGG 1 cut(s) 3089
Bse3DI GCAATG 5 cut(s) 2580, 5347, 5969, 6022, 6070
Bse8I GATNNNNATC 2 cut(s) 1248, 5997
BseBI CCWGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BseCI ATCGAT 2 cut(s) 3760, 6321
BseDI CCNNGG 8 cut(s) 17, 944, 3531, 3594, 5839, 6075, 6144, 6296
BseJI GATNNNNATC 2 cut(s) 1248, 5997
BseMI GCAATG 5 cut(s) 2580, 5347, 5969, 6022, 6070
BseRI GAGGAG 7 cut(s) 1391, 1394, 2283, 3011, 3157, 3611, 4472
BseSI GKGCMC 2 cut(s) 3865, 3955
BsgI GTGCAG 6 cut(s) 924, 1728, 4907, 5721, 5769, 6261
Bsh1236I CGCG 1 cut(s) 3262
BshFI GGCC 3 cut(s) 62, 91, 6444
BshNI GGYRCC 3 cut(s) 2957, 5765, 5776
BshVI ATCGAT 2 cut(s) 3760, 6321
BsiHKAI GWGCWC 3 cut(s) 3496, 3865, 4258
BsiHKCI CYCGRG 1 cut(s) 3593
BsiSI CCGG 1 cut(s) 5399
BslFI GGGAC 3 cut(s) 464, 1970, 4424
BsmBI CGTCTC 1 cut(s) 828
BsmFI GGGAC 3 cut(s) 464, 1970, 4424
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 62, 91, 6444
Bso31I GGTCTC 2 cut(s) 2328, 6123
BsoBI CYCGRG 1 cut(s) 3593
Bsp119I TTCGAA 2 cut(s) 3388, 5476
Bsp1286I GDGCHC 4 cut(s) 3496, 3865, 3955, 4258
Bsp1407I TGTACA 2 cut(s) 1267, 5251
Bsp19I CCATGG 1 cut(s) 6075
BspANI GGCC 3 cut(s) 62, 91, 6444
BspDI ATCGAT 2 cut(s) 3760, 6321
BspFNI CGCG 1 cut(s) 3262
BspHI TCATGA 4 cut(s) 34, 1917, 4840, 5023
BspMAI CTGCAG 5 cut(s) 1437, 3031, 4192, 4330, 4336
BspMI ACCTGC 5 cut(s) 3385, 4195, 4579, 5796, 6241
BspOI GCTAGC 2 cut(s) 2589, 5088
BspPI GGATC 7 cut(s) 114, 3576, 3589, 4232, 4694, 5028, 5443
BspQI GCTCTTC 5 cut(s) 249, 861, 981, 4246, 5454
BspT104I TTCGAA 2 cut(s) 3388, 5476
BspT107I GGYRCC 3 cut(s) 2957, 5765, 5776
BspTI CTTAAG 2 cut(s) 1805, 2792
BspTNI GGTCTC 2 cut(s) 2328, 6123
BsrDI GCAATG 5 cut(s) 2580, 5347, 5969, 6022, 6070
BsrGI TGTACA 2 cut(s) 1267, 5251
BssECI CCNNGG 8 cut(s) 17, 944, 3531, 3594, 5839, 6075, 6144, 6296
BssT1I CCWWGG 3 cut(s) 17, 3531, 6075
Bst2UI CCWGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BstAFI CTTAAG 2 cut(s) 1805, 2792
BstAPI GCANNNNNTGC 3 cut(s) 2609, 3035, 3254
BstAUI TGTACA 2 cut(s) 1267, 5251
BstBAI YACGTR 2 cut(s) 1820, 3785
BstBI TTCGAA 2 cut(s) 3388, 5476
BstDSI CCRYGG 2 cut(s) 6075, 6144
BstEII GGTNACC 1 cut(s) 4849
BstENI CCTNNNNNAGG 4 cut(s) 483, 3842, 4245, 4788
BstFNI CGCG 1 cut(s) 3262
BstH2I RGCGCY 4 cut(s) 55, 1265, 3208, 3269
BstHHI GCGC 7 cut(s) 54, 1264, 2079, 2274, 3207, 3262, 3268
BstNI CCWGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
BstNSI RCATGY 6 cut(s) 214, 1950, 2531, 3026, 3163, 4046
BstPI GGTNACC 1 cut(s) 4849
BstSCI CCNGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
BstSFI CTRYAG 7 cut(s) 816, 1433, 2539, 3027, 4188, 4326, 4332
BstSLI GKGCMC 2 cut(s) 3865, 3955
BstUI CGCG 1 cut(s) 3262
BstV2I GAAGAC 5 cut(s) 1578, 1916, 2580, 4833, 6157
BstX2I RGATCY 4 cut(s) 721, 1739, 3581, 5435
BstXI CCANNNNNNTGG 2 cut(s) 3773, 5214
BstYI RGATCY 4 cut(s) 721, 1739, 3581, 5435
Bsu15I ATCGAT 2 cut(s) 3760, 6321
Bsu36I CCTNAGG 1 cut(s) 3089
BsuI GTATCC 1 cut(s) 509
BsuRI GGCC 3 cut(s) 62, 91, 6444
BsuTUI ATCGAT 2 cut(s) 3760, 6321
BtgI CCRYGG 2 cut(s) 6075, 6144
BtgZI GCGATG 1 cut(s) 2924
BtrI CACGTC 1 cut(s) 5290
BtsI GCAGTG 2 cut(s) 4440, 5538
BveI ACCTGC 5 cut(s) 3385, 4195, 4579, 5796, 6241
CaiI CAGNNNCTG 2 cut(s) 1465, 3704
CciI TCATGA 4 cut(s) 34, 1917, 4840, 5023
CfoI GCGC 7 cut(s) 54, 1264, 2079, 2274, 3207, 3262, 3268
ClaI ATCGAT 2 cut(s) 3760, 6321
CsiI ACCWGGT 2 cut(s) 1102, 3225
DraI TTTAAA 4 cut(s) 1491, 2487, 4744, 5937
DriI GACNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 610
EciI GGCGGA 1 cut(s) 3579
Ecl136II GAGCTC 1 cut(s) 4256
Eco130I CCWWGG 3 cut(s) 17, 3531, 6075
Eco147I AGGCCT 1 cut(s) 6444
Eco24I GRGCYC 1 cut(s) 4258
Eco31I GGTCTC 2 cut(s) 2328, 6123
Eco32I GATATC 1 cut(s) 5333
Eco47III AGCGCT 1 cut(s) 3206
Eco53kI GAGCTC 1 cut(s) 4256
Eco57I CTGAAG 8 cut(s) 636, 1674, 2730, 3860, 4664, 4772, 4929, 6009
Eco72I CACGTG 1 cut(s) 3785
Eco81I CCTNAGG 1 cut(s) 3089
Eco88I CYCGRG 1 cut(s) 3593
Eco91I GGTNACC 1 cut(s) 4849
EcoICRI GAGCTC 1 cut(s) 4256
EcoNI CCTNNNNNAGG 4 cut(s) 483, 3842, 4245, 4788
EcoO109I RGGNCCY 3 cut(s) 61, 1967, 4411
EcoO65I GGTNACC 1 cut(s) 4849
EcoRI GAATTC 5 cut(s) 2369, 2836, 3046, 3905, 5139
EcoRII CCWGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
EcoRV GATATC 1 cut(s) 5333
EcoT14I CCWWGG 3 cut(s) 17, 3531, 6075
EcoT22I ATGCAT 2 cut(s) 2129, 2334
EcoT38I GRGCYC 1 cut(s) 4258
ErhI CCWWGG 3 cut(s) 17, 3531, 6075
Esp3I CGTCTC 1 cut(s) 828
FaqI GGGAC 3 cut(s) 464, 1970, 4424
FbaI TGATCA 3 cut(s) 31, 3807, 5071
FblI GTMKAC 3 cut(s) 2542, 2787, 4970
FriOI GRGCYC 1 cut(s) 4258
GlaI GCGC 7 cut(s) 53, 1263, 2078, 2273, 3206, 3261, 3267
GsuI CTGGAG 2 cut(s) 927, 1362
HaeII RGCGCY 4 cut(s) 55, 1265, 3208, 3269
HaeIII GGCC 3 cut(s) 62, 91, 6444
HapII CCGG 1 cut(s) 5399
HhaI GCGC 7 cut(s) 54, 1264, 2079, 2274, 3207, 3262, 3268
Hin6I GCGC 7 cut(s) 52, 1262, 2077, 2272, 3205, 3260, 3266
HinP1I GCGC 7 cut(s) 52, 1262, 2077, 2272, 3205, 3260, 3266
HincII GTYRAC 4 cut(s) 491, 2283, 3660, 5425
HindII GTYRAC 4 cut(s) 491, 2283, 3660, 5425
HindIII AAGCTT 4 cut(s) 26, 3173, 3235, 3917
HpaI GTTAAC 2 cut(s) 2283, 5425
HpaII CCGG 1 cut(s) 5399
Hpy99I CGWCG 4 cut(s) 90, 1176, 1895, 2484
HspAI GCGC 7 cut(s) 52, 1262, 2077, 2272, 3205, 3260, 3266
KflI GGGWCCC 1 cut(s) 4411
KpnI GGTACC 3 cut(s) 2961, 5769, 5780
Ksp22I TGATCA 3 cut(s) 31, 3807, 5071
KspAI GTTAAC 2 cut(s) 2283, 5425
LguI GCTCTTC 5 cut(s) 249, 861, 981, 4246, 5454
MabI ACCWGGT 2 cut(s) 1102, 3225
MfeI CAATTG 6 cut(s) 1923, 2697, 2735, 4695, 5856, 6458
MflI RGATCY 4 cut(s) 721, 1739, 3581, 5435
MhlI GDGCHC 4 cut(s) 3496, 3865, 3955, 4258
MlyI GAGTC 8 cut(s) 158, 1610, 2239, 2538, 4823, 5048, 5659, 6386
MmeI TCCRAC 4 cut(s) 1012, 4596, 5673, 5853
Mph1103I ATGCAT 2 cut(s) 2129, 2334
MroXI GAANNNNTTC 5 cut(s) 2976, 5534, 6038, 6208, 6423
MslI CAYNNNNRTG 9 cut(s) 1019, 1317, 2526, 3277, 3509, 3566, 5123, 5259, 5352
MspA1I CMGCKG 5 cut(s) 3032, 3619, 4331, 5555, 5783
MspCI CTTAAG 2 cut(s) 1805, 2792
MspI CCGG 1 cut(s) 5399
MspR9I CCNGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
MunI CAATTG 6 cut(s) 1923, 2697, 2735, 4695, 5856, 6458
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
MvnI CGCG 1 cut(s) 3262
NcoI CCATGG 1 cut(s) 6075
NheI GCTAGC 2 cut(s) 2585, 5084
NsiI ATGCAT 2 cut(s) 2129, 2334
NspI RCATGY 6 cut(s) 214, 1950, 2531, 3026, 3163, 4046
NspV TTCGAA 2 cut(s) 3388, 5476
PaeI GCATGC 2 cut(s) 214, 4046
PagI TCATGA 4 cut(s) 34, 1917, 4840, 5023
PaqCI CACCTGC 1 cut(s) 5796
PceI AGGCCT 1 cut(s) 6444
PciSI GCTCTTC 5 cut(s) 249, 861, 981, 4246, 5454
PcsI WCGNNNNNNNCGW 2 cut(s) 2488, 3766
PctI GAATGC 1 cut(s) 556
PdmI GAANNNNTTC 5 cut(s) 2976, 5534, 6038, 6208, 6423
PflMI CCANNNNNTGG 1 cut(s) 6081
PleI GAGTC 8 cut(s) 157, 1610, 2239, 2538, 4823, 5047, 5659, 6385
PmaCI CACGTG 1 cut(s) 3785
PmlI CACGTG 1 cut(s) 3785
PpsI GAGTC 8 cut(s) 157, 1610, 2239, 2538, 4823, 5047, 5659, 6385
Ppu21I YACGTR 2 cut(s) 1820, 3785
PpuMI RGGWCCY 2 cut(s) 1967, 4411
PshBI ATTAAT 1 cut(s) 2910
PsiI TTATAA 2 cut(s) 2645, 3327
Psp124BI GAGCTC 1 cut(s) 4258
Psp5II RGGWCCY 2 cut(s) 1967, 4411
Psp6I CCWGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
PspCI CACGTG 1 cut(s) 3785
PspEI GGTNACC 1 cut(s) 4849
PspGI CCWGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
PspPPI RGGWCCY 2 cut(s) 1967, 4411
PstI CTGCAG 5 cut(s) 1437, 3031, 4192, 4330, 4336
PstNI CAGNNNCTG 2 cut(s) 1465, 3704
PsuI RGATCY 4 cut(s) 721, 1739, 3581, 5435
PvuII CAGCTG 4 cut(s) 3032, 4331, 5555, 5783
RseI CAYNNNNRTG 9 cut(s) 1019, 1317, 2526, 3277, 3509, 3566, 5123, 5259, 5352
SacI GAGCTC 1 cut(s) 4258
SapI GCTCTTC 5 cut(s) 249, 861, 981, 4246, 5454
ScaI AGTACT 1 cut(s) 2189
SchI GAGTC 8 cut(s) 158, 1610, 2239, 2538, 4823, 5048, 5659, 6386
ScrFI CCNGG 7 cut(s) 885, 945, 1104, 2769, 3227, 5840, 6429
SduI GDGCHC 4 cut(s) 3496, 3865, 3955, 4258
SexAI ACCWGGT 2 cut(s) 1102, 3225
SfcI CTRYAG 7 cut(s) 816, 1433, 2539, 3027, 4188, 4326, 4332
SfuI TTCGAA 2 cut(s) 3388, 5476
SmiI ATTTAAAT 1 cut(s) 4744
SmiMI CAYNNNNRTG 9 cut(s) 1019, 1317, 2526, 3277, 3509, 3566, 5123, 5259, 5352
SmlI CTYRAG 7 cut(s) 392, 1715, 1805, 2792, 3052, 3753, 4961
SmoI CTYRAG 7 cut(s) 392, 1715, 1805, 2792, 3052, 3753, 4961
SpeI ACTAGT 1 cut(s) 1991
SphI GCATGC 2 cut(s) 214, 4046
SseBI AGGCCT 1 cut(s) 6444
SspI AATATT 5 cut(s) 2965, 3501, 3796, 5179, 5473
SstI GAGCTC 1 cut(s) 4258
StuI AGGCCT 1 cut(s) 6444
StyD4I CCNGG 7 cut(s) 883, 943, 1102, 2767, 3225, 5838, 6427
StyI CCWWGG 3 cut(s) 17, 3531, 6075
SwaI ATTTAAAT 1 cut(s) 4744
TaqI TCGA 9 cut(s) 147, 290, 3388, 3760, 4727, 5476, 6194, 6321, 6447
TaqII GACCGA 1 cut(s) 6111
TatI WGTACW 4 cut(s) 794, 1267, 2187, 5251
TauI GCSGC 5 cut(s) 1076, 1727, 4322, 5498, 5735
TspGWI ACGGA 3 cut(s) 2468, 5006, 6166
Van91I CCANNNNNTGG 1 cut(s) 6081
Vha464I CTTAAG 2 cut(s) 1805, 2792
VneI GTGCAC 1 cut(s) 3861
VspI ATTAAT 1 cut(s) 2910
XagI CCTNNNNNAGG 4 cut(s) 483, 3842, 4245, 4788
XbaI TCTAGA 2 cut(s) 4399, 5186
XceI RCATGY 6 cut(s) 214, 1950, 2531, 3026, 3163, 4046
XcmI CCANNNNNNNNNTGG 4 cut(s) 99, 1411, 3279, 4135
XmiI GTMKAC 3 cut(s) 2542, 2787, 4970
XmnI GAANNNNTTC 5 cut(s) 2976, 5534, 6038, 6208, 6423
ZrmI AGTACT 1 cut(s) 2189
Zsp2I ATGCAT 2 cut(s) 2129, 2334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.