Rh5AG179000
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014066
Physical Location & Seq
Forward (+)
20183023 .. 20195911
12889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG179000.1

Sequence Viewer

Length: 5466 bp
ATGGCCTCCAAAGGTCCAAGGCCGAAGCTCGATCACGAACCCAGACCCAAGCGCCACAAGGCCCTTGAAGTTGCAAATGAACCTCGACGGCCAAAAGCTCATTGGGACCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAGTCGGAGAGAAAATGGAAATTGGCTCAGGCAAAGAAGGTTGCTTTACGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAAGAAAATGAAGGAAGAAGAGCAACGGATGAAAAAAGTTGCACTTAATATTTCAAAGGATGTGAAGAAATTCTGGCTGAAAATAGAAAAACTGGTGCTTTACAAACATCAGATGGAGCTTGATGAGAAAAAGAAAAAGGCGCTTGATAAACAGCTTGAGTTCCTTCTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGCGGACAAGCCTGTGCAGCAATGTTCCACACAAGATCAACTGAGTATTGAAGGCACAGCTGTGGGCGAAAATGATATTAGAAAATCAGCAGAATTGAATGTCGAACCTCAATCAGATACAGCAGATGGTGATGATGATTATAATATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAGACGGGAAGAATTGGCTGCTTTACAGAATGAAGTGGATGTTCCACTCGAGGAGCTACTCAAACAATATGCTAGAAAGAAAGTTAACACAGAAAGTAGTCAAGAAAAGGATGAAGATGATGTTGCAGAGACGACTTCTTCTGGGGAAGATGATGGTATATCTCCTGAAAAGGGTGAAGATGACACTGAAATGACCTCAGTGGGGAAAGATCATAGTATGAGTTCAGAAAAGGGTGAAGATGGTGCAGAAGTCATGTCTGTGGGGGAAGATCATGATATTTGTCATAAAATGGGTGAAGTTGGCGCAGAAATGACCTCATTGAGTGAAGATCATGGTGAACAAAACGATCTTCTTGAAGCCAGTAAGACTGATAGAAGCAGCCCAGATGTTTTTACCGGTCGTCGTTGTGTTGGAAGTAATGGTGTGCCTATTTCAGAAACCCATTCATCCGAAATTAAGATTGGTGAAGCCAAAAATATTTCAGAAGCGTCTCTGCAGTCTGCCAAAGGAAATATGCCATATGATTTTGATGATGAACATGAGGATGGTGACTTCATTCTTGCTGCTGGAGAAGAAAAGGATGATGAGACAACCTTATTAGAGGAGGAGGAATTGGCAAAAGCAGATACCAATCATCCCATGGATGAGATTGCATTACTGCAGAAGGAGAGTGAAATACCGTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAGCGGTGATGAAGTTGCAGATGGTGAATCTGAATATGGTTCTGCTTTATCAGAGGGATTTACGGATTCTCCATCTCTAGAAGATTCTGAACTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCAACTGAAGAACATCGGGCTTCAGAAGGAGGAAGTGATAGTGAGAACAGAATCGAGGATGCAGCTGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACCTTCTCCACAACAAAAGTCCGTACAAAGTTCCCCTTTCTTCTGAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACAATGTATGAGAAAAGGCTGAATGGGATTCTAGCTGACGAGATGGGGCTAGGGAAGACAATCATGACAATCGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGCCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACAGAGTTTCTAAAATGGTGCCCTGCTTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGCAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCATTTCATGTCTGCATAACAACGTATAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCGCAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGCTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTCAGTAATCCAATATCCGGGATGGTTGAGGGGCAAGAAAAAGTAAATAAAGAAGTTCTTGATCGTCTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAACGAGATGTGGAGAAACAGCTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTTTCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGCTCAGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCACCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTTACATGCAACTGAGTTCTTCAATATGTTCAATGCTTTCGCCTGGACCATTTTCTGTGGTAGACCTTAAGGGATTGGGGTTTTTATTTACTCATCTAGATTTTTCGATGTCTTCTTGGGAGAGTGATGAAGTTAAAGCTCTTGCTACCCCATCAAGTTTAATTAAAGAGCGTGTTACCCGGATTCATCTTGAAGATATTGGTGGATTTAAACATCGTAAACATCACAAGAAGATGCATGGTATGAATATCTTCGAAGACATCCAGAAGGCACTCATGGAGGAGAGACTAAGGCAAGCAAAAGAACATGCTGCAGCCGTTGCGTGGTGGAATTCCTTGAGGTGTGATAGAAAACCTATATACTCAACGAGCCTGAGGGATCTTGTTACAGTTAGACATCCTGTTGTTGATATTGCTCATCACAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAGTTCTTTCACCAGTAGAACGCTTCCAGAAAACAATTGACCTGGTTGAAAGCTTCGTGTTTGCAATTCCAGCTGCACGAACTGCCCCACCTGTATGCTGGTGCAGTAAATCTGAAAGTTCTGTGTTTCTGCAACCAACTTATAAGCAGAAATGCTCTGAAGTCTTGTCACCACTTCTGTCACCATTTAGACCTGCAATCGTCCGTAGACAAGTATATTTTCCTGATAGGAGACTAATACAGTTTGACTGTGGTAAGTTGCAGCAGCTTGCAGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACGAAGATGCTTGACGTCTTGGAGGCTTTCATCAATTTATATGGTTACACATACATGCGTTTAGATGGATCCACTCCGCCTGAGGAGAGGCAGACTTTAATGCAGCGGTTTAACACAAACCCGAAATTTTTTCTTTTCATATTGTCAACTCGTAGTGGAGGTGTTGGTATTAACCTAGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGTTGCCACCGGATTGGACAGACACGAGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATCTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTAGTTATACAAAGTGGGGACTATAACACTGAATTCTTCAAAAAGCTTGATCCCATGGAGCTGTTTTCTGGTCATAGAGCGCTTCCCATTAAGAACATGCAAAAGGAGAAAAATCACAACACAACTGAAGTTTCTCTATCTAATGTGGACCTGGAAGCTGCACTAAAGCATGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTTGAGCAGGAAGAAGCTGTGGACAACCAAGAATTTACTGAAGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGACGACATGAAGGTTGATGAACCCACAGATCAGGGGGCTTTGATGATGATGTCTTCAAACAAAGACAGCGGAATGATGTTGAATGGGAGTGATCCTAATGAAGAAAGAGCTCTCACTGGTGCTTGTAGGGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCAGCCGCAGGACAAGAGATCTCATCCTTTGGAAATCAGTTACGTCCGATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAATAGACAAGACAGCAGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAACTGGACCGCATAGAAAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTTGTATATGAAACATGGGATGCTGAATTTGCAACTGAGGCATATCGGCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGAGAAGGAAGATGAAGCAGATGAAAATCTTGATTCTCTGAGGTATGGGATGCCAAGTGATCCTAAACCCAAGCATAAGAAGAAATCGAAGAAAGCAAAATTTAAATCTCTGAAGAAACGTTCTCTAGCTTCTGAGCTGGAACCTGTTAAAGGAGAGCTGCAAGTGGAACCTATGTTCATAGATGAAGATTATCTCTCTAATGATGTGTTTTCCTATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGCAAGAAGGCTGAATCTAAACCAGCTGGTGAAGATAAGATTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCTTGAAATCAGTACTTCAGAGTTTGATACTAGTGTCTCCTCAGTGCAGCATGTTGAAGCTTCAGAGTTAAAACCATGCGAGAGTGTGGTTGAATTCGAGCATAAACCAATTAGTAGAACCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCGGATTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGTTTGGTGAGTGAGACACTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCATTGTTGTGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTCTCCACTCCAGACAATCCTAATTATGAAAAGGTCAACAATATAGGATCTGGAAAAGCTCTTTTAAGAGTAACAGAGGTACATCTTAGTTTTCTTTTGAAATTTTCTTTTCACTTGCTGAATTATACATATGAATGTTTTGTTATCTTCATAGGCATTCATAATTTTTGTAGATAG

Protein Analysis

1821

Amino Acids

208.51

Weight (kDa)

5.4

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 93 3e-14 HSA domain
ResIII PF04851 563 - 724 2e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 568 - 853 1.9e-89 SNF2-related domain
DEAD PF00270 585 - 725 6.4e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1109 - 1221 1.9e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 588, 3213
AatII GACGTC 1 cut(s) 3417
Acc36I ACCTGC 2 cut(s) 3271, 4477
AccB1I GGYRCC 1 cut(s) 1908
AccB7I CCANNNNNTGG 1 cut(s) 4516
AccBSI CCGCTC 1 cut(s) 1386
AccI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
AclI AACGTT 1 cut(s) 4660
AclWI GGATC 8 cut(s) 616, 2988, 3462, 3475, 3803, 4136, 4595, 5344
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 9 cut(s) 305, 2932, 3524, 3791, 3998, 4460, 4640, 4967, 5390
AcyI GRCGYC 1 cut(s) 3414
AfaI GTAC 9 cut(s) 422, 1663, 2167, 2494, 3050, 4391, 4888, 5154, 5370
AfeI AGCGCT 1 cut(s) 3840
AfiI CCNNNNNNNGG 7 cut(s) 836, 867, 2276, 2925, 3580, 4516, 4691
AflII CTTAAG 1 cut(s) 2669
AflIII ACRYGT 1 cut(s) 1483
AgeI ACCGGT 2 cut(s) 1061, 2162
AhdI GACNNNNNGTC 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 4904
AjnI CCWGG 4 cut(s) 2426, 2644, 3111, 3909
AjuI GAANNNNNNNTTGG 4 cut(s) 1110, 1142, 2566, 2598
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 4 cut(s) 2123, 2155, 4700, 4732
Alw21I GWGCWC 2 cut(s) 3707, 4162
AlwI GGATC 8 cut(s) 616, 2988, 3462, 3475, 3803, 4136, 4595, 5344
AlwNI CAGNNNCTG 1 cut(s) 1607
Ama87I CYCGRG 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 3840
AoxI GGCC 6 cut(s) 3, 20, 60, 89, 1843, 5164
ApaI GGGCCC 1 cut(s) 1847
ApoI RAATTY 9 cut(s) 305, 2932, 3524, 3791, 3998, 4460, 4640, 4967, 5390
ArsI GACNNNNNNTTYG 4 cut(s) 1789, 1821, 4835, 4867
AsiGI ACCGGT 2 cut(s) 1061, 2162
Asp700I GAANNNNTTC 2 cut(s) 305, 2852
AspLEI GCGC 5 cut(s) 54, 379, 971, 1956, 3841
AsuC2I CCSGG 2 cut(s) 2278, 2782
AsuII TTCGAA 1 cut(s) 2856
AsuNHI GCTAGC 1 cut(s) 2462
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
AxyI CCTNAGG 2 cut(s) 2975, 3480
BaeGI GKGCMC 2 cut(s) 1847, 1913
BaeI ACNNNNGTAYC 4 cut(s) 4893, 4926, 5144, 5177
BamHI GGATCC 1 cut(s) 3467
BanI GGYRCC 1 cut(s) 1908
BanII GRGCYC 2 cut(s) 1847, 4162
BauI CACGAG 1 cut(s) 3669
BbsI GAAGAC 6 cut(s) 1793, 2035, 2457, 2706, 2865, 4095
Bbv12I GWGCWC 2 cut(s) 3707, 4162
BceAI ACGGC 3 cut(s) 104, 2343, 2903
BciT130I CCWGG 4 cut(s) 2428, 2646, 3113, 3911
BclI TGATCA 1 cut(s) 3693
BcnI CCSGG 2 cut(s) 2278, 2782
BcuI ACTAGT 1 cut(s) 4904
BfmI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
BfoI RGCGCY 3 cut(s) 55, 380, 3842
BfrI CTTAAG 1 cut(s) 2669
BfuAI ACCTGC 2 cut(s) 3271, 4477
BglII AGATCT 1 cut(s) 4242
BmcAI AGTACT 1 cut(s) 4888
Bme1390I CCNGG 6 cut(s) 2278, 2428, 2646, 2782, 3113, 3911
Bme18I GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 713
BmrFI CCNGG 6 cut(s) 2278, 2428, 2646, 2782, 3113, 3911
BmsI GCATC 7 cut(s) 1588, 2208, 2826, 3396, 4444, 4581, 5122
BmtI GCTAGC 1 cut(s) 2466
BpiI GAAGAC 6 cut(s) 1793, 2035, 2457, 2706, 2865, 4095
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 2 cut(s) 1254, 5283
Bpu10I CCTNAGC 2 cut(s) 174, 3350
Bpu14I TTCGAA 1 cut(s) 2856
BpuEI CTTGAG 4 cut(s) 413, 2959, 3624, 4326
BpuMI CCSGG 2 cut(s) 2278, 2782
Bsa29I ATCGAT 1 cut(s) 3688
BsaBI GATNNNNATC 2 cut(s) 1296, 4566
BsaHI GRCGYC 1 cut(s) 3414
BsaI GGTCTC 1 cut(s) 2205
BsaJI CCNNGG 4 cut(s) 17, 1305, 3813, 4539
BsaWI WCCGGW 4 cut(s) 1061, 2162, 3654, 5107
Bsc4I CCNNNNNNNGG 7 cut(s) 836, 867, 2276, 2925, 3580, 4516, 4691
Bse118I RCCGGY 2 cut(s) 1061, 2162
Bse21I CCTNAGG 2 cut(s) 2975, 3480
Bse3DI GCAATG 1 cut(s) 473
Bse8I GATNNNNATC 2 cut(s) 1296, 4566
BseBI CCWGG 4 cut(s) 2428, 2646, 3113, 3911
BseCI ATCGAT 1 cut(s) 3688
BseDI CCNNGG 4 cut(s) 17, 1305, 3813, 4539
BseJI GATNNNNATC 2 cut(s) 1296, 4566
BseLI CCNNNNNNNGG 7 cut(s) 836, 867, 2276, 2925, 3580, 4516, 4691
BseMI GCAATG 1 cut(s) 473
BseRI GAGGAG 9 cut(s) 731, 1283, 1286, 2160, 2897, 3043, 3497, 4370, 4903
BseSI GKGCMC 2 cut(s) 1847, 1913
BsgI GTGCAG 7 cut(s) 482, 930, 3129, 3193, 3903, 4808, 4940
Bsh1285I CGRYCG 1 cut(s) 1066
BshFI GGCC 6 cut(s) 5, 22, 62, 91, 1845, 5166
BshNI GGYRCC 1 cut(s) 1908
BshTI ACCGGT 2 cut(s) 1061, 2162
BshVI ATCGAT 1 cut(s) 3688
BsiEI CGRYCG 1 cut(s) 1066
BsiHKAI GWGCWC 2 cut(s) 3707, 4162
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
BslFI GGGAC 4 cut(s) 119, 1847, 3791, 4322
BslI CCNNNNNNNGG 7 cut(s) 836, 867, 2276, 2925, 3580, 4516, 4691
BsmBI CGTCTC 2 cut(s) 788, 1161
BsmFI GGGAC 4 cut(s) 119, 1847, 3791, 4322
BsmI GAATGC 1 cut(s) 5445
BsnI GGCC 6 cut(s) 5, 22, 62, 91, 1845, 5166
Bso31I GGTCTC 1 cut(s) 2205
BsoBI CYCGRG 1 cut(s) 713
Bsp119I TTCGAA 1 cut(s) 2856
Bsp120I GGGCCC 1 cut(s) 1843
Bsp1286I GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
Bsp19I CCATGG 2 cut(s) 1305, 3813
BspANI GGCC 6 cut(s) 5, 22, 62, 91, 1845, 5166
BspDI ATCGAT 1 cut(s) 3688
BspHI TCATGA 2 cut(s) 937, 1794
BspMAI CTGCAG 4 cut(s) 1164, 1329, 2917, 4225
BspMI ACCTGC 2 cut(s) 3271, 4477
BspOI GCTAGC 1 cut(s) 2466
BspPI GGATC 8 cut(s) 616, 2988, 3462, 3475, 3803, 4136, 4595, 5344
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 2856
BspT107I GGYRCC 1 cut(s) 1908
BspTI CTTAAG 1 cut(s) 2669
BspTNI GGTCTC 1 cut(s) 2205
BsrBI CCGCTC 1 cut(s) 1386
BsrDI GCAATG 1 cut(s) 473
BsrFI RCCGGY 2 cut(s) 1061, 2162
BssAI RCCGGY 2 cut(s) 1061, 2162
BssECI CCNNGG 4 cut(s) 17, 1305, 3813, 4539
BssNI GRCGYC 1 cut(s) 3414
BssSI CACGAG 1 cut(s) 3669
BssT1I CCWWGG 4 cut(s) 17, 1305, 3813, 4539
Bst2BI CACGAG 1 cut(s) 3669
Bst2UI CCWGG 4 cut(s) 2428, 2646, 3113, 3911
Bst4CI ACNGT 6 cut(s) 1347, 2417, 2992, 3312, 3320, 3592
Bst6I CTCTTC 4 cut(s) 114, 249, 1698, 1958
BstACI GRCGYC 1 cut(s) 3414
BstAFI CTTAAG 1 cut(s) 2669
BstAPI GCANNNNNTGC 3 cut(s) 1607, 2921, 3152
BstBI TTCGAA 1 cut(s) 2856
BstDSI CCRYGG 2 cut(s) 1305, 3813
BstENI CCTNNNNNAGG 1 cut(s) 4689
BstH2I RGCGCY 3 cut(s) 55, 380, 3842
BstHHI GCGC 5 cut(s) 54, 379, 971, 1956, 3841
BstMCI CGRYCG 1 cut(s) 1066
BstNI CCWGG 4 cut(s) 2428, 2646, 3113, 3911
BstNSI RCATGY 9 cut(s) 214, 1487, 2408, 2611, 2912, 3457, 3859, 3932, 4928
BstSCI CCNGG 6 cut(s) 2276, 2426, 2644, 2780, 3111, 3909
BstSFI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
BstSLI GKGCMC 2 cut(s) 1847, 1913
BstV2I GAAGAC 6 cut(s) 1793, 2035, 2457, 2706, 2865, 4095
BstX2I RGATCY 4 cut(s) 2980, 3467, 4242, 5336
BstXI CCANNNNNNTGG 1 cut(s) 3659
BstYI RGATCY 4 cut(s) 2980, 3467, 4242, 5336
Bsu15I ATCGAT 1 cut(s) 3688
Bsu36I CCTNAGG 2 cut(s) 2975, 3480
BsuRI GGCC 6 cut(s) 5, 22, 62, 91, 1845, 5166
BsuTUI ATCGAT 1 cut(s) 3688
BtgI CCRYGG 2 cut(s) 1305, 3813
BtsI GCAGTG 1 cut(s) 5019
BtsIMutI CAGTG 8 cut(s) 849, 870, 1876, 3703, 3786, 4164, 4923, 5019
BveI ACCTGC 2 cut(s) 3271, 4477
CaiI CAGNNNCTG 1 cut(s) 1607
CciI TCATGA 2 cut(s) 937, 1794
CfoI GCGC 5 cut(s) 54, 379, 971, 1956, 3841
Cfr10I RCCGGY 2 cut(s) 1061, 2162
ClaI ATCGAT 1 cut(s) 3688
CseI GACGC 2 cut(s) 1143, 2157
CsiI ACCWGGT 1 cut(s) 3111
Csp6I GTAC 9 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153, 5369
CspAI ACCGGT 2 cut(s) 1061, 2162
CviQI GTAC 9 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153, 5369
DraI TTTAAA 2 cut(s) 2812, 4645
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 4 cut(s) 114, 249, 1698, 1958
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 4 cut(s) 114, 249, 1698, 1958
EciI GGCGGA 3 cut(s) 2330, 2345, 3465
Ecl136II GAGCTC 1 cut(s) 4160
Eco130I CCWWGG 4 cut(s) 17, 1305, 3813, 4539
Eco24I GRGCYC 2 cut(s) 1847, 4162
Eco31I GGTCTC 1 cut(s) 2205
Eco32I GATATC 1 cut(s) 5234
Eco47I GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
Eco47III AGCGCT 1 cut(s) 3840
Eco53kI GAGCTC 1 cut(s) 4160
Eco81I CCTNAGG 2 cut(s) 2975, 3480
Eco88I CYCGRG 1 cut(s) 713
EcoICRI GAGCTC 1 cut(s) 4160
EcoNI CCTNNNNNAGG 1 cut(s) 4689
EcoO109I RGGNCCY 4 cut(s) 61, 1843, 1844, 4309
EcoRI GAATTC 3 cut(s) 2932, 3791, 4967
EcoRII CCWGG 4 cut(s) 2426, 2644, 3111, 3909
EcoRV GATATC 1 cut(s) 5234
EcoT14I CCWWGG 4 cut(s) 17, 1305, 3813, 4539
EcoT22I ATGCAT 2 cut(s) 2211, 2841
EcoT38I GRGCYC 2 cut(s) 1847, 4162
ErhI CCWWGG 4 cut(s) 17, 1305, 3813, 4539
Esp3I CGTCTC 2 cut(s) 788, 1161
FalI AAGNNNNNCTT 8 cut(s) 264, 296, 1658, 1690, 2301, 2333, 4419, 4451
FaqI GGGAC 4 cut(s) 119, 1847, 3791, 4322
FauNDI CATATG 3 cut(s) 1186, 1838, 5419
FbaI TGATCA 1 cut(s) 3693
FblI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
FriOI GRGCYC 2 cut(s) 1847, 4162
GlaI GCGC 5 cut(s) 53, 378, 970, 1955, 3840
GsuI CTGGAG 2 cut(s) 1254, 5283
HaeII RGCGCY 3 cut(s) 55, 380, 3842
HaeIII GGCC 6 cut(s) 5, 22, 62, 91, 1845, 5166
HapII CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
HgaI GACGC 2 cut(s) 1143, 2157
HhaI GCGC 5 cut(s) 54, 379, 971, 1956, 3841
Hin1I GRCGYC 1 cut(s) 3414
Hin6I GCGC 5 cut(s) 52, 377, 969, 1954, 3839
HinP1I GCGC 5 cut(s) 52, 377, 969, 1954, 3839
HincII GTYRAC 4 cut(s) 751, 1507, 3546, 5326
HindII GTYRAC 4 cut(s) 751, 1507, 3546, 5326
HindIII AAGCTT 4 cut(s) 3059, 3121, 3803, 4932
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
Hpy99I CGWCG 3 cut(s) 90, 1071, 2151
HpyCH4III ACNGT 6 cut(s) 1347, 2417, 2992, 3312, 3320, 3592
HpyCH4IV ACGT 4 cut(s) 2012, 3414, 4267, 4660
HpySE526I ACGT 4 cut(s) 2012, 3414, 4267, 4660
Hsp92I GRCGYC 1 cut(s) 3414
HspAI GCGC 5 cut(s) 52, 377, 969, 1954, 3839
KflI GGGWCCC 1 cut(s) 4309
Ksp22I TGATCA 1 cut(s) 3693
KspAI GTTAAC 1 cut(s) 751
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 352, 718, 3817
LweI GCATC 7 cut(s) 1588, 2208, 2826, 3396, 4444, 4581, 5122
MabI ACCWGGT 1 cut(s) 3111
MaeII ACGT 4 cut(s) 2012, 3414, 4267, 4660
MbiI CCGCTC 1 cut(s) 1386
MfeI CAATTG 3 cut(s) 1882, 2574, 3105
MflI RGATCY 4 cut(s) 2980, 3467, 4242, 5336
MhlI GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
MlyI GAGTC 4 cut(s) 158, 1502, 3742, 4495
MmeI TCCRAC 3 cut(s) 132, 1057, 1565
Mph1103I ATGCAT 2 cut(s) 2211, 2841
MroXI GAANNNNTTC 2 cut(s) 305, 2852
MspA1I CMGCKG 7 cut(s) 506, 1604, 2386, 3143, 3505, 4119, 4823
MspCI CTTAAG 1 cut(s) 2669
MspI CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
MspR9I CCNGG 6 cut(s) 2278, 2428, 2646, 2782, 3113, 3911
MunI CAATTG 3 cut(s) 1882, 2574, 3105
Mva1269I GAATGC 1 cut(s) 5445
MvaI CCWGG 4 cut(s) 2428, 2646, 3113, 3911
NciI CCSGG 2 cut(s) 2278, 2782
NcoI CCATGG 2 cut(s) 1305, 3813
NdeI CATATG 3 cut(s) 1186, 1838, 5419
NheI GCTAGC 1 cut(s) 2462
NmuCI GTSAC 5 cut(s) 1214, 3237, 3249, 3371, 3608
NsiI ATGCAT 2 cut(s) 2211, 2841
NspI RCATGY 9 cut(s) 214, 1487, 2408, 2611, 2912, 3457, 3859, 3932, 4928
NspV TTCGAA 1 cut(s) 2856
OliI CACNNNNGTG 1 cut(s) 506
PacI TTAATTAA 1 cut(s) 2766
PaeI GCATGC 2 cut(s) 214, 3932
PaeR7I CTCGAG 1 cut(s) 713
PagI TCATGA 2 cut(s) 937, 1794
PciI ACATGT 1 cut(s) 1483
PciSI GCTCTTC 1 cut(s) 249
PctI GAATGC 1 cut(s) 5445
PdmI GAANNNNTTC 2 cut(s) 305, 2852
PflMI CCANNNNNTGG 1 cut(s) 4516
PfoI TCCNGGA 2 cut(s) 2276, 2426
PinAI ACCGGT 2 cut(s) 1061, 2162
PleI GAGTC 4 cut(s) 157, 1502, 3742, 4495
PpsI GAGTC 4 cut(s) 157, 1502, 3742, 4495
PpuMI RGGWCCY 1 cut(s) 4309
PscI ACATGT 1 cut(s) 1483
PsiI TTATAA 2 cut(s) 588, 3213
Psp124BI GAGCTC 1 cut(s) 4162
Psp1406I AACGTT 1 cut(s) 4660
Psp5II RGGWCCY 1 cut(s) 4309
Psp6I CCWGG 4 cut(s) 2426, 2644, 3111, 3909
PspGI CCWGG 4 cut(s) 2426, 2644, 3111, 3909
PspOMI GGGCCC 1 cut(s) 1843
PspPPI RGGWCCY 1 cut(s) 4309
PspXI VCTCGAGB 1 cut(s) 713
PsrI GAACNNNNNNTAC 2 cut(s) 5025, 5057
PstI CTGCAG 4 cut(s) 1164, 1329, 2917, 4225
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 4 cut(s) 2980, 3467, 4242, 5336
PvuII CAGCTG 5 cut(s) 506, 1604, 2386, 3143, 4823
RsaI GTAC 9 cut(s) 422, 1663, 2167, 2494, 3050, 4391, 4888, 5154, 5370
RsaNI GTAC 9 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153, 5369
SacI GAGCTC 1 cut(s) 4162
SapI GCTCTTC 1 cut(s) 249
ScaI AGTACT 1 cut(s) 4888
SchI GAGTC 4 cut(s) 158, 1502, 3742, 4495
ScrFI CCNGG 6 cut(s) 2278, 2428, 2646, 2782, 3113, 3911
SduI GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
SexAI ACCWGGT 1 cut(s) 3111
SfaNI GCATC 7 cut(s) 1588, 2208, 2826, 3396, 4444, 4581, 5122
SfcI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
Sfr274I CTCGAG 1 cut(s) 713
SfuI TTCGAA 1 cut(s) 2856
SinI GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
SlaI CTCGAG 1 cut(s) 713
SmiI ATTTAAAT 1 cut(s) 4645
SmlI CTYRAG 6 cut(s) 392, 713, 2669, 2938, 3639, 4341
SmoI CTYRAG 6 cut(s) 392, 713, 2669, 2938, 3639, 4341
SpeI ACTAGT 1 cut(s) 4904
SphI GCATGC 2 cut(s) 214, 3932
SspI AATATT 2 cut(s) 286, 1144
SstI GAGCTC 1 cut(s) 4162
StyD4I CCNGG 6 cut(s) 2276, 2426, 2644, 2780, 3111, 3909
StyI CCWWGG 4 cut(s) 17, 1305, 3813, 4539
SwaI ATTTAAAT 1 cut(s) 4645
TaaI ACNGT 6 cut(s) 1347, 2417, 2992, 3312, 3320, 3592
TaiI ACGT 4 cut(s) 2015, 3417, 4270, 4663
TatI WGTACW 3 cut(s) 3048, 4389, 4886
TauI GCSGC 1 cut(s) 4232
TscAI CASTG 8 cut(s) 856, 870, 1876, 3703, 3793, 4171, 4923, 5026
TseFI GTSAC 5 cut(s) 1214, 3237, 3249, 3371, 3608
Tsp45I GTSAC 5 cut(s) 1214, 3237, 3249, 3371, 3608
TspGWI ACGGA 4 cut(s) 277, 1460, 1649, 3263
TspRI CASTG 8 cut(s) 856, 870, 1876, 3703, 3793, 4171, 4923, 5026
Van91I CCANNNNNTGG 1 cut(s) 4516
Vha464I CTTAAG 1 cut(s) 2669
VpaK11BI GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
XagI CCTNNNNNAGG 1 cut(s) 4689
XapI RAATTY 9 cut(s) 305, 2932, 3524, 3791, 3998, 4460, 4640, 4967, 5390
XbaI TCTAGA 4 cut(s) 1459, 2698, 4297, 5087
XceI RCATGY 9 cut(s) 214, 1487, 2408, 2611, 2912, 3457, 3859, 3932, 4928
XcmI CCANNNNNNNNNTGG 4 cut(s) 99, 1303, 3165, 4021
XhoI CTCGAG 1 cut(s) 713
XmiI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
XmnI GAANNNNTTC 2 cut(s) 305, 2852
ZraI GACGTC 1 cut(s) 3415
ZrmI AGTACT 1 cut(s) 4888
Zsp2I ATGCAT 2 cut(s) 2211, 2841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.