FvH4_3g15170
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014066
Physical Location & Seq
Forward (+)
9420872 .. 9435273
14402 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15170.t1

Sequence Viewer

Length: 6351 bp
ATGGCCTCCAAAGGTCCTAGGCCGAAGCTCGATCATGAAACCAGGCCCAAGCGCCACAAGGCTCTTGAAGTTGCAAATGAACCTCGACGGCCAAAAGCTCATTGGGATCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAGTCAGAGAGAAAATGGAAATTGGCTCAAGCAAAGAAGGTTGCTTTAAGAGCGACCAAGGGCATGCTAGATCAGGCTACGAGGGGAGAAAAAAGAATGAAGGAAGAAGAGCAACGGATGAAAAAAGTTGCACTTAATATTTCAAAGGATGTGAAGAAATTCTGGTTGAAAATAGAAAAACTGGTGTTGTACAAACATCAGATGGAGCTCGATGAGAAAAAGAAAAAGGCACTTGATAAACAACTCGAGTTCCTTTTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGTGGACAAGCCTGTGCAGCAATGTTCCACACAAGTTCAACTGAGTATTGAAGGGGCAGCTGTGGGCGAAAATGATATTAGCAAATCAGCAGAATTGAATGTTGAACCTCAATCAGATACAGCGGACGGCGATGATGATTATGATATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAGGCAGGAAGAGTTGGCTGCTTTACAGAATGAGGTGGATGTTCCACTCGAGCAGCTACTGAAACAATATTCCAGGAAGAGAGTTAACACAGAAGTTAGCGAAGAAAAGAGTAAAGATGTTGCAAAGATGACATCTTCTGAAGAAGATGACGGCATGTCTCCTAAAAAGGGTGAAGATGACACTGAAATGACCTCATCGGGGAAAGACCACAGTATTTGTTCCGAAATGGGTGAAGATGGTGCAGAAATTTTGTCTGTGGGGGAAGATCATGATATGTGTCTTAAAAAGGGTGAAGTTGGCGCAGAAATGACCTCAGTGAGTGAAGATCATGGTGAACAAAACAATCTTATAGCCAGTAAGACTGATAGAAGCAGCCCAGATGTTTTTACCGGTCGTCGTTGTGTTGGAAATAATGGCTTGCCTATTTCAGAAACCCATTTATCAGAAATTAAGATTGGTGAAGCCAAAAACATTTCAGAAGCATCTCGGCAGTCTGCCAAAGGACATGTACCATATGATTTTGATGATGAACATGAAGATGGTGACTTCATTCTTGCTGCTGGAGAAGAAAAGGATGATGAGACAACGTTATTAGAGGAGGAGGAATTAGCAAAAGCAGATACCAATGATCCTTCGGATGAGATTGCATTACTGCAGAAGGAGAGTGAAATACCTTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAACAGCGATGAAGTTGAAGATGATGAATCTGAATATGATTCTGCTTTATCAGAGGGATTTATGGATTCTCCATCTCCAGGAGATTCACAAGTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACAGCAGCCAGCTCTTGATTCTCCAACTGAAGAATGTAGGGCTTCAGAAGGTGGAAGTGATAGTGAGAATAGAATTGAGGATGCAGCCGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACATTCTCCACAACAAAAGTCCGTACAAAGTTCCCCTTTCTTCTAAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACGATGTATGAGAAAAAGCTGAACGGGATTCTAGCCGACGAGATGGGGTTAGGGAAGACAATCATGACAATAGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGTCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACTGAGTTTCTAAAGTGGTGCCCCGCGTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGCAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCCTTTCATGTCTGCATAACAACGTACAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCACAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGTTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCGCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTTAGTAATCCAATATCTGGGATGGTTGAGGGGCAAGAGAAAGTAAATAAAGAAGTTCTTGATCGTTTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAACGAGATGTGGAGAAACAACTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTATCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGTTCGGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCACCCTGACTTGTTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTAACATGCAACTGAGTTCGTCAATATGTTCAATGCTTTCGCCTGGACCATTTTCTATGGTAGACCTTAAGGGTTTGGGGTTTGTATTTAGTCATCTAGATTTTACCATGACTTCTTGGGAGAGTGATGAAGTTAAGGCTCTTGCTACCCCATCAAGTTTAATCAAAGACCGTGTTGACCTGATCCATCTTGTAGATATTGGTGGATTTAAACATCATAAACATCATAAGAAGATGCATGGTATGAATATTTTCGAAGACATCCAGAGGGCACTTATGGAGGAGAGACTAAGGCAAGCAAAAGAACATGCTGCAGCAGTTGCATGGTGGAATTCCTTGAGGTGTGACAGAAAACCTATATATTCAACAAGCCTGAGGGATCTTGTTACAGTTAGACATCCTGTTGTTGAAGTTGCTCATTGCAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAATTCTTTCACCAGTAGAACGATTCCAGAAAACAATTGACCTGGTTGAAAGCTTTGTCTTTGCAATCCCTGCTGCACGAGCCGCCCCACCTGTATGTTGGTGCAGTAAATCTGAAAGTCCTGTGTTCCTGCAATCAACTTATAAGCAGAAATGTTCTCAAGTCCTATCGCCACTTCTGTCACCATTTAGACCTGCAATTGTCCGCAGACAAGTATATTTTCCTGATAGGCGACTAATACAGTTTGACTGTGGCAAGTTGCAGCAGCTTGCAGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACAAAGATGCTTGACGTCTTGGAGGCTTTCATCAATCTATATGGTTACACGTACATGCGTTTGGATGGATCCACTCCGCCGGAGGAGAGACAGACGTTAATGCAGCGGTTTAACACAAACCCAAAGTTTTTCCTTTTCATATTGTCGACTCGTAGTGGAGGTGTTGGTATCAACCTGGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAGCAGGCTCAAGATCGTTGCCACCGTATTGGACAGACACGAGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATTTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTTGTTATACAAAGTGGGGACTACAACACGGAGTTCTTCAAAAAGCTTGATCCTATGGAGCTTTTTTCTGGTCATAGAGCCCTTCCCATTAAGAATATGCAAAAGGAGAAAAATCACAACGCAACTGAAGTTTCTCTATCTAATGTAGACCTCGAAGCTGCACTAAAGCAGGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTTGAACAGGAAGAAGCTGTGGACAACCAAGAATTTACTGAAGAAGCCGTTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGACGACATGAAGGTTGATGAACCCACAGATCAGGGGGCTTTGATGATATCTTCAAACAAAGACAACGGAATGATGTTGAATGTGAGTGATCCTAATGAAGAAAGAAGTCTCACTGTTGCTTGTAGGGAAGATGATGCGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCTGCTGCTGCCGCAGGACAAGAGATCTCATCCTTTGAGAATCAGTTACGTCCAATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAGTAGACAAGACAGCAGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAATTGGACCGCATAGAAAAATACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTTGTATATGAAACATGGGATGCTGAGTTTGCAACTGAGGCATATCGTCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGTGAAGGAAGATGAAGCAGTTGAAAACCTTGATTCTCAGAGATATGGGATGCCAAGTGATCCTAAACCCAAGAAAAAGAAGAAATCGAAGAAAGCAAAATTCAAATCTCTGAAGAAACGGTCTCTAGCTTCTGAACTGGAACCTGTTAAAGAAGAACTGCAAGTGGAACCTATGTATATTGATGAAGATTATCTCTCTAATGAAGCGCTTTCGCATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGTAAGAAGGCATCATCTAAACCAGCTGGTGAAGAAAAGAGTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCTGGAAATCTGTACTCCAGAGTTCGAAACTAGTGTCTCCTCACTGCACCATGTTGAAGCTTCAGAGTTAAAACCATGTGATAGTGTGGTTGAATTTGAGCATAAACCAATTAGTAGAACCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCTGACTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGCTTGGTGAGTGAGACACTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCATTGTTGCGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTATCTGCACCAGACAACCCTAATAATGAAAAGGTTAACAATATAGGATCTGGAAAAGCTCTCTTAAGGGTAACAGAGGAAAATATTCGAATGTTATTAAATGTTGCCGCGGAGCAGCCCAATACAGAATTTTTGATTCAGAGACACTTCGTTGCCCTGCTATCCTCTGTCTGGAAGATGGCATCTCACAAAGATGGCAAACAAAATCTCCCCTCTTCTGGGAATGGTGTATATTTGGGTGGAAATTTTTTCAGTTCTTCCAACCAAATTTCTCGGACTTCAGTGAAGGAAAACACAGCAACCATGAAATTTACCAATTGTGGACAGGGTGCCAGGTTGGTAGCAGCTGCACTTAATGATGCCAGCAGCAAGCAAGAGGATGAGAGTGTCTTTTCCCCCAACCCTAGGAAAAAATCGTCAACTGATGCTGAACAGTTGGATATAATTCTGGAGTTTCAGGCTCAAACAGATGCCTCCATGGATCCTTTTCCATCTGTTATCAATTTGTCAATATCTGGTTCAGGCCGTCCACCAGAAAATATGGCAATGGAACCGAATTTTCTCAGGGAATCCTGTAATGACAAGGATGCAAATGTGGCTGAGAATCGTTTCAGGAATGCAACAAGAGCTTGTGATGAAGATAACATGGGATGGGCTTCATCGACATTCCCGACATATGATGTCAGGTCTCGAACAATGTCAAAGTTACCTTCTTCAGGAAAGCACAAGCTTGTTTTCTCCGATCCAATTAGACCTTCGAAATCCAAGTTCCGGAAAAATGCAGTTGAGCATAGTGAGATGCGTCAAATTATGGCTGAGCAAGTGTTCCCACCTTTCTCAATAGCTGCACCCCTGAATCCCAGTCCAAGGTTTGACCTGAACCTACCTGTCAACGAGGATACTGAAACTGATGATCTGGAAAGCAATTCCCATTCTCAGGTGGTCGAGTCTTCATTTGAAGAAAGCTTTGGAGTTCTCCCACACGAGTATGTTCCTGGTTTACTTTCAGGTCTCGATGACTGTTCGTTGTTGCAAGAATGTGATGAATATGATGACATCGCGTAA

Protein Analysis

2117

Amino Acids

240.63

Weight (kDa)

5.31

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 93 8.5e-15 HSA domain
ResIII PF04851 561 - 722 2e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 566 - 851 1.6e-89 SNF2-related domain
DEAD PF00270 583 - 723 7.3e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1107 - 1219 2.3e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3207
AatII GACGTC 1 cut(s) 3411
Acc36I ACCTGC 2 cut(s) 3265, 4474
AccB1I GGYRCC 2 cut(s) 1902, 5615
AccB7I CCANNNNNTGG 2 cut(s) 2270, 4513
AccI GTMKAC 5 cut(s) 2413, 2658, 3539, 3900, 4317
AccII CGCG 3 cut(s) 1910, 5396, 6347
AccIII TCCGGA 1 cut(s) 6057
AclI AACGTT 1 cut(s) 1253
AcoI YGGCCR 1 cut(s) 89
AcyI GRCGYC 1 cut(s) 3408
AfeI AGCGCT 1 cut(s) 4746
AflII CTTAAG 2 cut(s) 2663, 5350
AflIII ACRYGT 3 cut(s) 1171, 1477, 3441
AgeI ACCGGT 2 cut(s) 1055, 2156
AhdI GACNNNNNGTC 2 cut(s) 148, 820
AhlI ACTAGT 1 cut(s) 4901
AjnI CCWGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
AjuI GAANNNNNNNTTGG 8 cut(s) 1104, 1136, 2560, 2592, 6025, 6057, 6237, 6269
AloI GAACNNNNNNTCC 2 cut(s) 2117, 2149
Alw21I GWGCWC 2 cut(s) 357, 3701
AlwNI CAGNNNCTG 3 cut(s) 724, 1601, 4220
Ama87I CYCGRG 2 cut(s) 392, 713
Aor13HI TCCGGA 1 cut(s) 6057
Aor51HI AGCGCT 1 cut(s) 4746
AoxI GGCC 6 cut(s) 3, 20, 44, 89, 5161, 5809
ArsI GACNNNNNNTTYG 6 cut(s) 1783, 1815, 4832, 4864, 4890, 4922
AsiGI ACCGGT 2 cut(s) 1055, 2156
Asp700I GAANNNNTTC 4 cut(s) 305, 2846, 5370, 5894
AspA2I CCTAGG 2 cut(s) 17, 5690
AspLEI GCGC 4 cut(s) 54, 968, 1950, 4747
AspS9I GGNCC 9 cut(s) 14, 45, 1838, 1855, 2642, 3622, 4306, 4372, 5162
AsuII TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
AvaI CYCGRG 2 cut(s) 392, 713
AvaII GGWCC 7 cut(s) 14, 1838, 1855, 2642, 3622, 4306, 4372
AvrII CCTAGG 2 cut(s) 17, 5690
AxyI CCTNAGG 1 cut(s) 2969
BaeGI GKGCMC 2 cut(s) 1907, 2869
BaeI ACNNNNGTAYC 2 cut(s) 5141, 5174
BamHI GGATCC 2 cut(s) 3461, 5767
BanI GGYRCC 2 cut(s) 1902, 5615
BanII GRGCYC 2 cut(s) 357, 3835
BauI CACGAG 3 cut(s) 3141, 3663, 6269
BbsI GAAGAC 5 cut(s) 1787, 2029, 2451, 2859, 6228
Bbv12I GWGCWC 2 cut(s) 357, 3701
BceAI ACGGC 6 cut(s) 104, 589, 832, 2337, 3992, 5796
BciT130I CCWGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
BciVI GTATCC 1 cut(s) 6178
BclI TGATCA 1 cut(s) 3687
BcuI ACTAGT 1 cut(s) 4901
BfmI CTRYAG 5 cut(s) 1319, 2168, 2410, 2907, 4212
BfoI RGCGCY 2 cut(s) 55, 4748
BfrI CTTAAG 2 cut(s) 2663, 5350
BfuAI ACCTGC 2 cut(s) 3265, 4474
BfuI GTATCC 1 cut(s) 6178
BglII AGATCT 1 cut(s) 4239
BlnI CCTAGG 2 cut(s) 17, 5690
BlpI GCTNAGC 1 cut(s) 6102
Bme1390I CCNGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
Bme18I GGWCC 7 cut(s) 14, 1838, 1855, 2642, 3622, 4306, 4372
BmeRI GACNNNNNGTC 2 cut(s) 148, 820
BmeT110I CYCGRG 2 cut(s) 392, 713
BmgT120I GGNCC 9 cut(s) 14, 45, 1838, 1855, 2642, 3622, 4306, 4372, 5162
BmrFI CCNGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
BmrI ACTGGG 1 cut(s) 6141
BmuI ACTGGG 1 cut(s) 6141
BpiI GAAGAC 5 cut(s) 1787, 2029, 2451, 2859, 6228
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 4 cut(s) 1248, 1437, 4872, 5756
Bpu10I CCTNAGC 1 cut(s) 3344
Bpu1102I GCTNAGC 1 cut(s) 6102
Bpu14I TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
BpuEI CTTGAG 5 cut(s) 159, 2953, 3207, 3618, 4323
Bsa29I ATCGAT 1 cut(s) 3682
BsaAI YACGTR 1 cut(s) 3444
BsaHI GRCGYC 1 cut(s) 3408
BsaI GGTCTC 4 cut(s) 2199, 4665, 5979, 6302
BsaJI CCNNGG 7 cut(s) 17, 204, 4536, 5394, 5690, 5763, 6152
BsaWI WCCGGW 3 cut(s) 1055, 2156, 6057
Bse118I RCCGGY 2 cut(s) 1055, 2156
Bse21I CCTNAGG 1 cut(s) 2969
Bse3DI GCAATG 3 cut(s) 473, 3013, 5838
BseAI TCCGGA 1 cut(s) 6057
BseBI CCWGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
BseCI ATCGAT 1 cut(s) 3682
BseDI CCNNGG 7 cut(s) 17, 204, 4536, 5394, 5690, 5763, 6152
BseMI GCAATG 3 cut(s) 473, 3013, 5838
BseRI GAGGAG 8 cut(s) 1277, 1280, 2154, 2891, 3037, 3491, 4367, 4900
BseSI GKGCMC 2 cut(s) 1907, 2869
Bsh1236I CGCG 3 cut(s) 1910, 5396, 6347
Bsh1285I CGRYCG 1 cut(s) 1060
BshFI GGCC 6 cut(s) 5, 22, 46, 91, 5163, 5811
BshNI GGYRCC 2 cut(s) 1902, 5615
BshTI ACCGGT 2 cut(s) 1055, 2156
BshVI ATCGAT 1 cut(s) 3682
BsiEI CGRYCG 1 cut(s) 1060
BsiHKAI GWGCWC 2 cut(s) 357, 3701
BsiHKCI CYCGRG 2 cut(s) 392, 713
BsiSI CCGG 4 cut(s) 1056, 2157, 3473, 6058
BslFI GGGAC 3 cut(s) 1841, 3785, 4319
BsmFI GGGAC 3 cut(s) 1841, 3785, 4319
BsmI GAATGC 2 cut(s) 4753, 5908
BsnI GGCC 6 cut(s) 5, 22, 46, 91, 5163, 5811
Bso31I GGTCTC 4 cut(s) 2199, 4665, 5979, 6302
BsoBI CYCGRG 2 cut(s) 392, 713
Bsp119I TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
Bsp1286I GDGCHC 5 cut(s) 357, 1907, 2869, 3701, 3835
Bsp13I TCCGGA 1 cut(s) 6057
Bsp1407I TGTACA 1 cut(s) 336
Bsp1720I GCTNAGC 1 cut(s) 6102
Bsp19I CCATGG 1 cut(s) 5763
BspANI GGCC 6 cut(s) 5, 22, 46, 91, 5163, 5811
BspDI ATCGAT 1 cut(s) 3682
BspEI TCCGGA 1 cut(s) 6057
BspFNI CGCG 3 cut(s) 1910, 5396, 6347
BspHI TCATGA 3 cut(s) 34, 934, 1788
BspMAI CTGCAG 3 cut(s) 1323, 2911, 4216
BspMI ACCTGC 2 cut(s) 3265, 4474
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
BspT107I GGYRCC 2 cut(s) 1902, 5615
BspTI CTTAAG 2 cut(s) 2663, 5350
BspTNI GGTCTC 4 cut(s) 2199, 4665, 5979, 6302
BsrDI GCAATG 3 cut(s) 473, 3013, 5838
BsrFI RCCGGY 2 cut(s) 1055, 2156
BsrGI TGTACA 1 cut(s) 336
BssAI RCCGGY 2 cut(s) 1055, 2156
BssECI CCNNGG 7 cut(s) 17, 204, 4536, 5394, 5690, 5763, 6152
BssNI GRCGYC 1 cut(s) 3408
BssSI CACGAG 3 cut(s) 3141, 3663, 6269
BssT1I CCWWGG 6 cut(s) 17, 204, 4536, 5690, 5763, 6152
Bst2BI CACGAG 3 cut(s) 3141, 3663, 6269
Bst2UI CCWGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
Bst6I CTCTTC 7 cut(s) 114, 249, 669, 737, 1692, 1952, 5506
BstACI GRCGYC 1 cut(s) 3408
BstAFI CTTAAG 2 cut(s) 2663, 5350
BstAPI GCANNNNNTGC 4 cut(s) 1601, 2915, 3134, 4220
BstAUI TGTACA 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 3444
BstBI TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
BstDSI CCRYGG 2 cut(s) 5394, 5763
BstENI CCTNNNNNAGG 1 cut(s) 6000
BstFNI CGCG 3 cut(s) 1910, 5396, 6347
BstH2I RGCGCY 2 cut(s) 55, 4748
BstHHI GCGC 4 cut(s) 54, 968, 1950, 4747
BstMCI CGRYCG 1 cut(s) 1060
BstNI CCWGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
BstNSI RCATGY 8 cut(s) 214, 823, 1175, 1481, 2402, 2605, 2906, 3451
BstSCI CCNGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
BstSFI CTRYAG 5 cut(s) 1319, 2168, 2410, 2907, 4212
BstSLI GKGCMC 2 cut(s) 1907, 2869
BstUI CGCG 3 cut(s) 1910, 5396, 6347
BstV2I GAAGAC 5 cut(s) 1787, 2029, 2451, 2859, 6228
BstX2I RGATCY 5 cut(s) 2974, 3461, 4239, 5333, 5767
BstXI CCANNNNNNTGG 1 cut(s) 3653
BstYI RGATCY 5 cut(s) 2974, 3461, 4239, 5333, 5767
Bsu15I ATCGAT 1 cut(s) 3682
Bsu36I CCTNAGG 1 cut(s) 2969
BsuI GTATCC 1 cut(s) 6178
BsuRI GGCC 6 cut(s) 5, 22, 46, 91, 5163, 5811
BsuTUI ATCGAT 1 cut(s) 3682
BtgI CCRYGG 2 cut(s) 5394, 5763
BtgZI GCGATG 3 cut(s) 591, 1398, 6328
BtsI GCAGTG 2 cut(s) 4913, 5016
BtsIMutI CAGTG 8 cut(s) 846, 987, 1870, 3697, 4155, 4913, 5016, 5574
BveI ACCTGC 2 cut(s) 3265, 4474
CaiI CAGNNNCTG 3 cut(s) 724, 1601, 4220
CciI TCATGA 3 cut(s) 34, 934, 1788
CfoI GCGC 4 cut(s) 54, 968, 1950, 4747
Cfr10I RCCGGY 2 cut(s) 1055, 2156
Cfr13I GGNCC 9 cut(s) 14, 45, 1838, 1855, 2642, 3622, 4306, 4372, 5162
Cfr42I CCGCGG 1 cut(s) 5397
ClaI ATCGAT 1 cut(s) 3682
CseI GACGC 2 cut(s) 2151, 6077
CsiI ACCWGGT 2 cut(s) 3105, 3567
CspAI ACCGGT 2 cut(s) 1055, 2156
CspCI CAANNNNNGTGG 2 cut(s) 863, 898
DraI TTTAAA 1 cut(s) 2806
DriI GACNNNNNGTC 2 cut(s) 148, 820
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 7 cut(s) 114, 249, 669, 737, 1692, 1952, 5506
Eam1105I GACNNNNNGTC 2 cut(s) 148, 820
EarI CTCTTC 7 cut(s) 114, 249, 669, 737, 1692, 1952, 5506
EciI GGCGGA 3 cut(s) 2324, 2339, 3459
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 6 cut(s) 17, 204, 4536, 5690, 5763, 6152
Eco24I GRGCYC 2 cut(s) 357, 3835
Eco31I GGTCTC 4 cut(s) 2199, 4665, 5979, 6302
Eco32I GATATC 2 cut(s) 4092, 5231
Eco47I GGWCC 7 cut(s) 14, 1838, 1855, 2642, 3622, 4306, 4372
Eco47III AGCGCT 1 cut(s) 4746
Eco53kI GAGCTC 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 2969
Eco88I CYCGRG 2 cut(s) 392, 713
EcoICRI GAGCTC 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 6000
EcoO109I RGGNCCY 3 cut(s) 14, 1838, 4306
EcoRI GAATTC 1 cut(s) 2926
EcoRII CCWGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
EcoRV GATATC 2 cut(s) 4092, 5231
EcoT14I CCWWGG 6 cut(s) 17, 204, 4536, 5690, 5763, 6152
EcoT22I ATGCAT 2 cut(s) 2205, 2835
EcoT38I GRGCYC 2 cut(s) 357, 3835
ErhI CCWWGG 6 cut(s) 17, 204, 4536, 5690, 5763, 6152
FalI AAGNNNNNCTT 8 cut(s) 264, 296, 1652, 1684, 2295, 2327, 4416, 4448
FaqI GGGAC 3 cut(s) 1841, 3785, 4319
FauI CCCGC 1 cut(s) 1915
FauNDI CATATG 3 cut(s) 1180, 1832, 5962
FbaI TGATCA 1 cut(s) 3687
FblI GTMKAC 5 cut(s) 2413, 2658, 3539, 3900, 4317
FriOI GRGCYC 2 cut(s) 357, 3835
GlaI GCGC 4 cut(s) 53, 967, 1949, 4746
GsuI CTGGAG 4 cut(s) 1248, 1437, 4872, 5756
HaeII RGCGCY 2 cut(s) 55, 4748
HaeIII GGCC 6 cut(s) 5, 22, 46, 91, 5163, 5811
HapII CCGG 4 cut(s) 1056, 2157, 3473, 6058
HgaI GACGC 2 cut(s) 2151, 6077
HhaI GCGC 4 cut(s) 54, 968, 1950, 4747
Hin1I GRCGYC 1 cut(s) 3408
Hin6I GCGC 4 cut(s) 52, 966, 1948, 4745
HinP1I GCGC 4 cut(s) 52, 966, 1948, 4745
HincII GTYRAC 8 cut(s) 751, 1501, 2154, 2773, 3540, 5323, 5706, 6178
HindII GTYRAC 8 cut(s) 751, 1501, 2154, 2773, 3540, 5323, 5706, 6178
HindIII AAGCTT 6 cut(s) 3053, 3115, 3797, 4929, 6014, 6250
HpaI GTTAAC 3 cut(s) 751, 2154, 5323
HpaII CCGG 4 cut(s) 1056, 2157, 3473, 6058
Hpy99I CGWCG 4 cut(s) 90, 1065, 1766, 2145
HpyCH4IV ACGT 7 cut(s) 1253, 2006, 3408, 3443, 3488, 4264, 4795
HpySE526I ACGT 7 cut(s) 1253, 2006, 3408, 3443, 3488, 4264, 4795
Hsp92I GRCGYC 1 cut(s) 3408
HspAI GCGC 4 cut(s) 52, 966, 1948, 4745
KflI GGGWCCC 1 cut(s) 4306
Kpn2I TCCGGA 1 cut(s) 6057
Ksp22I TGATCA 1 cut(s) 3687
KspAI GTTAAC 3 cut(s) 751, 2154, 5323
KspI CCGCGG 1 cut(s) 5397
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 352, 3811, 5172, 5398
MabI ACCWGGT 2 cut(s) 3105, 3567
MaeII ACGT 7 cut(s) 1253, 2006, 3408, 3443, 3488, 4264, 4795
MfeI CAATTG 5 cut(s) 1876, 2568, 3099, 3261, 5602
MflI RGATCY 5 cut(s) 2974, 3461, 4239, 5333, 5767
MhlI GDGCHC 5 cut(s) 357, 1907, 2869, 3701, 3835
MlyI GAGTC 6 cut(s) 158, 1496, 3535, 3736, 4492, 6242
MmeI TCCRAC 4 cut(s) 1051, 1559, 5571, 5703
Mph1103I ATGCAT 2 cut(s) 2205, 2835
MroI TCCGGA 1 cut(s) 6057
MroXI GAANNNNTTC 4 cut(s) 305, 2846, 5370, 5894
MspA1I CMGCKG 8 cut(s) 506, 569, 1601, 3499, 4217, 4820, 5396, 5633
MspCI CTTAAG 2 cut(s) 2663, 5350
MspI CCGG 4 cut(s) 1056, 2157, 3473, 6058
MspR9I CCNGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
MunI CAATTG 5 cut(s) 1876, 2568, 3099, 3261, 5602
Mva1269I GAATGC 2 cut(s) 4753, 5908
MvaI CCWGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
MvnI CGCG 3 cut(s) 1910, 5396, 6347
NcoI CCATGG 1 cut(s) 5763
NdeI CATATG 3 cut(s) 1180, 1832, 5962
NmeAIII GCCGAG 1 cut(s) 1132
NmuCI GTSAC 6 cut(s) 1208, 2100, 2939, 3243, 3365, 3602
NsiI ATGCAT 2 cut(s) 2205, 2835
NspI RCATGY 8 cut(s) 214, 823, 1175, 1481, 2402, 2605, 2906, 3451
NspV TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
PaeI GCATGC 1 cut(s) 214
PaeR7I CTCGAG 2 cut(s) 392, 713
PagI TCATGA 3 cut(s) 34, 934, 1788
PciI ACATGT 2 cut(s) 1171, 1477
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 3646, 5954
PctI GAATGC 2 cut(s) 4753, 5908
PdmI GAANNNNTTC 4 cut(s) 305, 2846, 5370, 5894
PflMI CCANNNNNTGG 2 cut(s) 2270, 4513
PfoI TCCNGGA 3 cut(s) 737, 1453, 2420
PinAI ACCGGT 2 cut(s) 1055, 2156
PleI GAGTC 6 cut(s) 157, 1496, 3535, 3736, 4492, 6241
PpsI GAGTC 6 cut(s) 157, 1496, 3535, 3736, 4492, 6241
Ppu21I YACGTR 1 cut(s) 3444
PpuMI RGGWCCY 3 cut(s) 14, 1838, 4306
PscI ACATGT 2 cut(s) 1171, 1477
PsiI TTATAA 1 cut(s) 3207
Psp124BI GAGCTC 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 1253
Psp5II RGGWCCY 3 cut(s) 14, 1838, 4306
Psp6I CCWGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
PspGI CCWGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
PspPI GGNCC 9 cut(s) 14, 45, 1838, 1855, 2642, 3622, 4306, 4372, 5162
PspPPI RGGWCCY 3 cut(s) 14, 1838, 4306
PspXI VCTCGAGB 2 cut(s) 392, 713
PsrI GAACNNNNNNTAC 2 cut(s) 5022, 5054
PstI CTGCAG 3 cut(s) 1323, 2911, 4216
PstNI CAGNNNCTG 3 cut(s) 724, 1601, 4220
PsuI RGATCY 5 cut(s) 2974, 3461, 4239, 5333, 5767
PvuII CAGCTG 4 cut(s) 506, 4217, 4820, 5633
SacI GAGCTC 1 cut(s) 357
SacII CCGCGG 1 cut(s) 5397
SalI GTCGAC 1 cut(s) 3538
SapI GCTCTTC 1 cut(s) 249
Sau96I GGNCC 9 cut(s) 14, 45, 1838, 1855, 2642, 3622, 4306, 4372, 5162
SchI GAGTC 6 cut(s) 158, 1496, 3535, 3736, 4492, 6242
ScrFI CCNGG 9 cut(s) 43, 739, 1455, 2422, 2640, 3107, 3569, 5620, 6282
SduI GDGCHC 5 cut(s) 357, 1907, 2869, 3701, 3835
SexAI ACCWGGT 2 cut(s) 3105, 3567
SfcI CTRYAG 5 cut(s) 1319, 2168, 2410, 2907, 4212
Sfr274I CTCGAG 2 cut(s) 392, 713
Sfr303I CCGCGG 1 cut(s) 5397
SfuI TTCGAA 4 cut(s) 2850, 4896, 5374, 6044
SgrBI CCGCGG 1 cut(s) 5397
SinI GGWCC 7 cut(s) 14, 1838, 1855, 2642, 3622, 4306, 4372
SlaI CTCGAG 2 cut(s) 392, 713
SmlI CTYRAG 9 cut(s) 174, 392, 713, 2663, 2932, 3222, 3633, 4338, 5350
SmoI CTYRAG 9 cut(s) 174, 392, 713, 2663, 2932, 3222, 3633, 4338, 5350
SpeI ACTAGT 1 cut(s) 4901
SphI GCATGC 1 cut(s) 214
SspI AATATT 4 cut(s) 286, 734, 2845, 5371
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 9 cut(s) 41, 737, 1453, 2420, 2638, 3105, 3567, 5618, 6280
StyI CCWWGG 6 cut(s) 17, 204, 4536, 5690, 5763, 6152
TaiI ACGT 7 cut(s) 1256, 2009, 3411, 3446, 3491, 4267, 4798
TatI WGTACW 3 cut(s) 336, 3042, 4883
TauI GCSGC 5 cut(s) 1601, 2218, 3149, 4229, 5396
TscAI CASTG 8 cut(s) 853, 987, 1870, 3697, 4162, 4920, 5023, 5574
TseFI GTSAC 6 cut(s) 1208, 2100, 2939, 3243, 3365, 3602
Tsp45I GTSAC 6 cut(s) 1208, 2100, 2939, 3243, 3365, 3602
TspGWI ACGGA 4 cut(s) 277, 1643, 3797, 4125
TspRI CASTG 8 cut(s) 853, 987, 1870, 3697, 4162, 4920, 5023, 5574
Van91I CCANNNNNTGG 2 cut(s) 2270, 4513
Vha464I CTTAAG 2 cut(s) 2663, 5350
VpaK11BI GGWCC 7 cut(s) 14, 1838, 1855, 2642, 3622, 4306, 4372
XagI CCTNNNNNAGG 1 cut(s) 6000
XbaI TCTAGA 3 cut(s) 2692, 4294, 5084
XceI RCATGY 8 cut(s) 214, 823, 1175, 1481, 2402, 2605, 2906, 3451
XcmI CCANNNNNNNNNTGG 2 cut(s) 99, 3159
XhoI CTCGAG 2 cut(s) 392, 713
XmaJI CCTAGG 2 cut(s) 17, 5690
XmiI GTMKAC 5 cut(s) 2413, 2658, 3539, 3900, 4317
XmnI GAANNNNTTC 4 cut(s) 305, 2846, 5370, 5894
ZraI GACGTC 1 cut(s) 3409
Zsp2I ATGCAT 2 cut(s) 2205, 2835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.