Rw5G016250
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014066
Physical Location & Seq
Forward (+)
20373238 .. 20385137
11900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016250.1

Sequence Viewer

Length: 6360 bp
ATGGCCTCCAAAGGTCCAAGGCCGAAGCTCGATCACGAACCCAGACCCAAGCGCCACAAGGCCCTTGAAGTTGCAAATGAACCTCGACGGCCAAAAGCTCATTGGGACCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAGTCGGAGAGAAAATGGAAATTGGCTCAGGCAAAGAAGGTTGCTTTACGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAAGAAAATGAAGGAAGAAGAGCAACGGATGAAAAAAGTTGCACTTAATATTTCAAAGGATGTGAAGAAATTCTGGCTGAAAATAGAAAAACTGGTGCTTTACAAACATCAGATGGAGCTTGATGAGAAAAAGAAAAAGGCGCTTGATAAACAGCTTGAGTTCCTTCTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGCGGACAAGCCTGTGCAGCAATGTTCCACACAAGATCAACTGAGTATTGAAGGCACAGCTGTGGGCGAAAATGATATTAGAAAATCAGCAGAATTGAATGTCGAACCTCAATCAGATACAGCAGATGGTGATGATGATTATAATATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAGACGGGAAGAATTGGCTGCTTTACAGAATGAAGTGGATGTTCCACTCGAGGAGCTACTCAAACAATATGCTAGAAAGAAAGTTAACACAGAAAGTAGTCAAGAAAAGGATGAAGATGATGTTGCAGAGACGACTTCTTCTGGGGAAGATGATGGTATATCTCCTGAAAAGGGTGAAGATGACACTGAAATGACCTCAGTGGGGAAAGATCATAGTATGAGTTCAGAAAAGGGTGAAGATGGTGCAGAAGTCATGTCTGTGGGGGAAGATCATGATATTTGTCATAAAATGGGTGAAGTTGGCGCAGAAATGACCTCATTGAGTGAAGATCATGGTGAACAAAACGATCTTCTTGAAGCCAGTAAGACTGATAGAAGCAGCCCAGATGTTTTTACCGGTCGTCGTTGTGTTGGAAGTAATGGTGTGCCTATTTCAGAAACCCATTCATCCGAAATTAAGATTGGTGAAGCCAAAAATATTTCAGAAGCGTCTCTGCAGTCTGCCAAAGGAAATATGCCATATGATTTTGATGATGAACATGAGGATGGTGACTTCATTCTTGCTGCTGGAGAAGAAAAGGATGATGAGACAACCTTATTAGAGGAGGAGGAATTGGCAAAAGCAGATACCAATCATCCCATGGATGAGATTGCATTACTGCAGAAGGAGAGTGAAATACCGTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAGCGGTGATGAAGTTGCAGATGGTGAATCTGAATATGGTTCTGCTTTATCAGAGGGATTTACGGATTCTCCATCTCTAGAAGATTCTGAACTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCAACTGAAGAACATCGGGCTTCAGAAGGAGGAAGTGATAGTGAGAACAGAATCGAGGATGCAGCTGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACCTTCTCCACAACAAAAGTCCGTACAAAGTTCCCCTTTCTTCTGAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACAATGTATGAGAAAAGGCTGAATGGGATTCTAGCTGACGAGATGGGGCTAGGGAAGACAATCATGACAATCGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGCCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACAGAGTTTCTAAAATGGTGCCCTGCTTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGCAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCATTTCATGTCTGCATAACAACGTATAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCGCAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGCTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTCAGTAATCCAATATCCGGGATGGTTGAGGGGCAAGAAAAAGTAAATAAAGAAGTTCTTGATCGTCTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAACGAGATGTGGAGAAACAGCTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTTTCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGCTCAGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCACCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTTACATGCAACTGAGTTCTTCAATATGTTCAATGCTTTCGCCTGGACCATTTTCTGTGGTAGACCTTAAGGGATTGGGGTTTTTATTTACTCATCTAGATTTTTCGATGTCTTCTTGGGAGAGTGATGAAGTTAAAGCTCTTGCTACCCCATCAAGTTTAATTAAAGAGCGTGTTACCCGGATTCATCTTGAAGATATTGGTGGATTTAAACATCGTAAACATCACAAGAAGATGCATGGTATGAATATCTTCGAAGACATCCAGAAGGCACTCATGGAGGAGAGACTAAGGCAAGCAAAAGAACATGCTGCAGCCGTTGCGTGGTGGAATTCCTTGAGGTGTGATAGAAAACCTATATACTCAACGAGCCTGAGGGATCTTGTTACAGTTAGACATCCTGTTGTTGATATTGCTCATCACAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAGTTCTTTCACCAGTAGAACGCTTCCAGAAAACAATTGACCTGGTTGAAAGCTTCGTGTTTGCAATTCCAGCTGCACGAACTGCCCCACCTGTATGCTGGTGCAGTAAATCTGAAAGTTCTGTGTTTCTGCAACCAACTTATAAGCAGAAATGCTCTGAAGTCTTGTCACCACTTCTGTCACCATTTAGACCTGCAATCGTCCGTAGACAAGTATATTTTCCTGATAGGAGACTAATACAGTTTGACTGTGGTAAGTTGCAGCAGCTTGCAGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACGAAGATGCTTGACGTCTTGGAGGCTTTCATCAATTTATATGGTTACACATACATGCGTTTAGATGGATCCACTCCGCCTGAGGAGAGGCAGACTTTAATGCAGCGGTTTAACACAAACCCGAAATTTTTTCTTTTCATATTGTCAACTCGTAGTGGAGGTGTTGGTATTAACCTAGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGTTGCCACCGGATTGGACAGACACGAGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATCTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTAGTTATACAAAGTGGGGACTATAACACTGAATTCTTCAAAAAGCTTGATCCCATGGAGCTGTTTTCTGGTCATAGAGCGCTTCCCATTAAGAACATGCAAAAGGAGAAAAATCACAACACAACTGAAGTTTCTCTATCTAATGTGGACCTGGAAGCTGCACTAAAGCATGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTTGAGCAGGAAGAAGCTGTGGACAACCAAGAATTTACTGAAGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGACGACATGAAGGTTGATGAACCCACAGATCAGGGGGCCTTGATGATGATGTCTTCAAACAAAGACAGCGGAATGATGTTGAATGGGAGTGATCCTAATGAAGAAAGAGCTCTCACTGGTGCTTGTAGGGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCAGCCGCAGGACAAGAGATCTCATCCTTTGGAAATCAGTTACGTCCGATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAATAGACAAGACAGCAGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAACTGGACCGCATAGAAAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTTGTATATGAAACATGGGATGCTGAATTTGCAACTGAGGCATATCGGCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGAGAAGGAAGATGAAGCAGATGAAAATCTTGATTCTCTGAGGTATGGGATGCCAAGTGATCCTAAACCCAAGCATAAGAAGAAATCGAAGAAAGCAAAATTTAAATCTCTGAAGAAACGTTCTCTAGCTTCTGAGCTGGAACCTGTTAAAGGAGAGCTGCAAGTGGAACCTATGTTCATAGATGAAGATTATCTCTCTAATGATGTGTTTTCCTATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGCAAGAAGGCTGAATCTAAACCAGCTGGTGAAGATAAGATTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCTTGAAATCAGTACTTCAGAGTTTGATACTAGTGTCTCCTCAGTGCAGCATGTTGAAGCTTCAGAGTTAAAACCATGCGAGAGTGTGGTTGAATTCGAGCATAAACCAATTAGTAGAACCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCGGATTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGTTTGGTGAGTGAGACACTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCATTGTTGTGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTCTCCACTCCAGACAATCCTAATTATGAAAAGGTCAACAATATAGGATCTGGAAAAGCTCTTTTAAGAGTAACAGAGGAAAATATTCGAATGTTATTAAATGTTGCTGCCGAGCAGCCCAATACAGAATATTTGATTCAGAAACACTTCATTGCCCTGCTATCCTCTATCTGGAAGATGGCATCTCACAAAGACGGGAGACAAAATCTCCCATCTTCTGGGAATGGTGTATATTTTGGCAGAAATTTTTTCAGTTCTTCCAACCAAATTTCTCGGACTTCAGTGAAGGAAAACACAGCAACCATGAAATTTACCCATTTTGGACAGGGTGCCAGGTTGGTAGCAGCTGCGCTTAATGATACCAGCAGCAGGCGAGGGGATGAGTGTGTCATTTCTCCCAACCCTAGGAAAAAATCATCAACCGATGCTGAACAGTTGGATATAACTCTGGAGTTTCAGGCACAAAAGGATGACTCCACAGATCCATTTCCATCTGTTATCAATTTATCAATATCTGATATAGGCCCTCCATCAGAAAATAAGGCAACGGAACCGTATCTTCTCAGAGATTCCTGTAATGACTATTGCAAAAGCACCAGTGATGCAAATGTGGCTGAGAACCGTTTCAGGAATGCAACAAGAGCTTGCGATGAAGATACTATGGGGTGGGCTTCATCGACATTCCCGACATATGATGTCAGGTCTCGATCAGTGTCAAAGTTACCTTCCTCAGGAAAGCACAAGCTTGTCTTCTCCGATCCAGTTAGACCTTCAAAATCCAAGTTCAGAAAAAATGCAGTAGAGCATGGTGAGATGCGTCACTTTCTGGCTGAGCATGTATTCCCACCCTTGCCAATAGCTGCACCGCTGAATCCCAGTCCAAGGTTCGACCTGAACCTACCTGTCAACGAGGATATTGAGACTGGTGATCTGGAAAGCACTTCCCATTCTCAGGTGGTTGAGTCTTCATTTGAAGAAAGCTTTGGAGTTCTCCCACATGAATATGTTCCTGAATTGCTTTCAGGCCTTGATGACTGTTCATTATTGCCAGAATATGATGACATTGCCTAA

Protein Analysis

2119

Amino Acids

241.23

Weight (kDa)

5.34

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 93 3.6e-14 HSA domain
ResIII PF04851 563 - 724 2.4e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 568 - 853 2.4e-89 SNF2-related domain
DEAD PF00270 585 - 725 7.8e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1109 - 1221 2.3e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 588, 3213
AatII GACGTC 1 cut(s) 3417
Acc36I ACCTGC 2 cut(s) 3271, 4477
AccB1I GGYRCC 2 cut(s) 1908, 5618
AccB7I CCANNNNNTGG 2 cut(s) 4516, 5507
AccBSI CCGCTC 1 cut(s) 1386
AccI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
AclI AACGTT 1 cut(s) 4660
AcoI YGGCCR 1 cut(s) 89
AcyI GRCGYC 1 cut(s) 3414
AfaI GTAC 8 cut(s) 422, 1663, 2167, 2494, 3050, 4391, 4888, 5154
AfeI AGCGCT 1 cut(s) 3840
AflII CTTAAG 1 cut(s) 2669
AflIII ACRYGT 1 cut(s) 1483
AgeI ACCGGT 2 cut(s) 1061, 2162
AhdI GACNNNNNGTC 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 4904
AjnI CCWGG 5 cut(s) 2426, 2644, 3111, 3909, 5621
AjuI GAANNNNNNNTTGG 6 cut(s) 1110, 1142, 2566, 2598, 6255, 6287
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 4 cut(s) 2123, 2155, 4700, 4732
Alw21I GWGCWC 2 cut(s) 3707, 4162
AlwNI CAGNNNCTG 1 cut(s) 1607
Ama87I CYCGRG 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 3840
AoxI GGCC 9 cut(s) 3, 20, 60, 89, 1843, 4086, 5164, 5812, 6313
ApaI GGGCCC 1 cut(s) 1847
ArsI GACNNNNNNTTYG 4 cut(s) 1789, 1821, 4835, 4867
AsiGI ACCGGT 2 cut(s) 1061, 2162
Asp700I GAANNNNTTC 6 cut(s) 305, 2852, 5373, 5435, 5912, 6294
AspA2I CCTAGG 1 cut(s) 5693
AspLEI GCGC 6 cut(s) 54, 379, 971, 1956, 3841, 5641
AsuC2I CCSGG 2 cut(s) 2278, 2782
AsuII TTCGAA 2 cut(s) 2856, 5377
AsuNHI GCTAGC 1 cut(s) 2462
AvaI CYCGRG 1 cut(s) 713
AvaII GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
AvrII CCTAGG 1 cut(s) 5693
AxyI CCTNAGG 3 cut(s) 2975, 3480, 6019
BaeGI GKGCMC 2 cut(s) 1847, 1913
BaeI ACNNNNGTAYC 4 cut(s) 4893, 4926, 5144, 5177
BamHI GGATCC 1 cut(s) 3467
BanI GGYRCC 2 cut(s) 1908, 5618
BanII GRGCYC 2 cut(s) 1847, 4162
BauI CACGAG 1 cut(s) 3669
BbsI GAAGAC 8 cut(s) 1793, 2035, 2457, 2706, 2865, 4095, 6031, 6246
Bbv12I GWGCWC 2 cut(s) 3707, 4162
BceAI ACGGC 3 cut(s) 104, 2343, 2903
BciT130I CCWGG 5 cut(s) 2428, 2646, 3113, 3911, 5623
BclI TGATCA 1 cut(s) 3693
BcnI CCSGG 2 cut(s) 2278, 2782
BcuI ACTAGT 1 cut(s) 4904
BfmI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
BfoI RGCGCY 3 cut(s) 55, 380, 3842
BfrI CTTAAG 1 cut(s) 2669
BfuAI ACCTGC 2 cut(s) 3271, 4477
BglII AGATCT 1 cut(s) 4242
BlnI CCTAGG 1 cut(s) 5693
BlpI GCTNAGC 1 cut(s) 6120
BmcAI AGTACT 1 cut(s) 4888
Bme1390I CCNGG 7 cut(s) 2278, 2428, 2646, 2782, 3113, 3911, 5623
Bme18I GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 713
BmrFI CCNGG 7 cut(s) 2278, 2428, 2646, 2782, 3113, 3911, 5623
BmrI ACTGGG 1 cut(s) 6159
BmtI GCTAGC 1 cut(s) 2466
BmuI ACTGGG 1 cut(s) 6159
BpiI GAAGAC 8 cut(s) 1793, 2035, 2457, 2706, 2865, 4095, 6031, 6246
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 3 cut(s) 1254, 5283, 5759
Bpu10I CCTNAGC 2 cut(s) 174, 3350
Bpu1102I GCTNAGC 1 cut(s) 6120
Bpu14I TTCGAA 2 cut(s) 2856, 5377
BpuEI CTTGAG 4 cut(s) 413, 2959, 3624, 4326
BpuMI CCSGG 2 cut(s) 2278, 2782
Bsa29I ATCGAT 1 cut(s) 3688
BsaBI GATNNNNATC 2 cut(s) 1296, 4566
BsaHI GRCGYC 1 cut(s) 3414
BsaI GGTCTC 2 cut(s) 2205, 5997
BsaJI CCNNGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
BsaWI WCCGGW 4 cut(s) 1061, 2162, 3654, 5107
Bse118I RCCGGY 2 cut(s) 1061, 2162
Bse21I CCTNAGG 3 cut(s) 2975, 3480, 6019
Bse3DI GCAATG 3 cut(s) 473, 5439, 6351
Bse8I GATNNNNATC 2 cut(s) 1296, 4566
BseBI CCWGG 5 cut(s) 2428, 2646, 3113, 3911, 5623
BseCI ATCGAT 1 cut(s) 3688
BseDI CCNNGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
BseJI GATNNNNATC 2 cut(s) 1296, 4566
BseMI GCAATG 3 cut(s) 473, 5439, 6351
BseRI GAGGAG 9 cut(s) 731, 1283, 1286, 2160, 2897, 3043, 3497, 4370, 4903
BseSI GKGCMC 2 cut(s) 1847, 1913
BsgI GTGCAG 8 cut(s) 482, 930, 3129, 3193, 3903, 4808, 4940, 6135
Bsh1285I CGRYCG 1 cut(s) 1066
BshFI GGCC 9 cut(s) 5, 22, 62, 91, 1845, 4088, 5166, 5814, 6315
BshNI GGYRCC 2 cut(s) 1908, 5618
BshTI ACCGGT 2 cut(s) 1061, 2162
BshVI ATCGAT 1 cut(s) 3688
BsiEI CGRYCG 1 cut(s) 1066
BsiHKAI GWGCWC 2 cut(s) 3707, 4162
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
BslFI GGGAC 4 cut(s) 119, 1847, 3791, 4322
BsmBI CGTCTC 2 cut(s) 788, 1161
BsmFI GGGAC 4 cut(s) 119, 1847, 3791, 4322
BsmI GAATGC 1 cut(s) 5926
BsnI GGCC 9 cut(s) 5, 22, 62, 91, 1845, 4088, 5166, 5814, 6315
Bso31I GGTCTC 2 cut(s) 2205, 5997
BsoBI CYCGRG 1 cut(s) 713
Bsp119I TTCGAA 2 cut(s) 2856, 5377
Bsp120I GGGCCC 1 cut(s) 1843
Bsp1286I GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
Bsp1720I GCTNAGC 1 cut(s) 6120
Bsp19I CCATGG 2 cut(s) 1305, 3813
BspANI GGCC 9 cut(s) 5, 22, 62, 91, 1845, 4088, 5166, 5814, 6315
BspDI ATCGAT 1 cut(s) 3688
BspHI TCATGA 2 cut(s) 937, 1794
BspMAI CTGCAG 4 cut(s) 1164, 1329, 2917, 4225
BspMI ACCTGC 2 cut(s) 3271, 4477
BspOI GCTAGC 1 cut(s) 2466
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 2 cut(s) 2856, 5377
BspT107I GGYRCC 2 cut(s) 1908, 5618
BspTI CTTAAG 1 cut(s) 2669
BspTNI GGTCTC 2 cut(s) 2205, 5997
BsrBI CCGCTC 1 cut(s) 1386
BsrDI GCAATG 3 cut(s) 473, 5439, 6351
BsrFI RCCGGY 2 cut(s) 1061, 2162
BssAI RCCGGY 2 cut(s) 1061, 2162
BssECI CCNNGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
BssNI GRCGYC 1 cut(s) 3414
BssSI CACGAG 1 cut(s) 3669
BssT1I CCWWGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
Bst2BI CACGAG 1 cut(s) 3669
Bst2UI CCWGG 5 cut(s) 2428, 2646, 3113, 3911, 5623
Bst6I CTCTTC 4 cut(s) 114, 249, 1698, 1958
BstACI GRCGYC 1 cut(s) 3414
BstAFI CTTAAG 1 cut(s) 2669
BstAPI GCANNNNNTGC 3 cut(s) 1607, 2921, 3152
BstBI TTCGAA 2 cut(s) 2856, 5377
BstDSI CCRYGG 2 cut(s) 1305, 3813
BstENI CCTNNNNNAGG 2 cut(s) 4689, 6018
BstH2I RGCGCY 3 cut(s) 55, 380, 3842
BstHHI GCGC 6 cut(s) 54, 379, 971, 1956, 3841, 5641
BstMCI CGRYCG 1 cut(s) 1066
BstNI CCWGG 5 cut(s) 2428, 2646, 3113, 3911, 5623
BstSCI CCNGG 7 cut(s) 2276, 2426, 2644, 2780, 3111, 3909, 5621
BstSFI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
BstSLI GKGCMC 2 cut(s) 1847, 1913
BstV2I GAAGAC 8 cut(s) 1793, 2035, 2457, 2706, 2865, 4095, 6031, 6246
BstX2I RGATCY 5 cut(s) 2980, 3467, 4242, 5336, 5770
BstXI CCANNNNNNTGG 1 cut(s) 3659
BstYI RGATCY 5 cut(s) 2980, 3467, 4242, 5336, 5770
Bsu15I ATCGAT 1 cut(s) 3688
Bsu36I CCTNAGG 3 cut(s) 2975, 3480, 6019
BsuRI GGCC 9 cut(s) 5, 22, 62, 91, 1845, 4088, 5166, 5814, 6315
BsuTUI ATCGAT 1 cut(s) 3688
BtgI CCRYGG 2 cut(s) 1305, 3813
BtgZI GCGATG 1 cut(s) 5952
BtsI GCAGTG 1 cut(s) 5019
BveI ACCTGC 2 cut(s) 3271, 4477
CaiI CAGNNNCTG 1 cut(s) 1607
CciI TCATGA 2 cut(s) 937, 1794
CfoI GCGC 6 cut(s) 54, 379, 971, 1956, 3841, 5641
Cfr10I RCCGGY 2 cut(s) 1061, 2162
ClaI ATCGAT 1 cut(s) 3688
CseI GACGC 3 cut(s) 1143, 2157, 6095
CsiI ACCWGGT 1 cut(s) 3111
Csp6I GTAC 8 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153
CspAI ACCGGT 2 cut(s) 1061, 2162
CviQI GTAC 8 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153
DraI TTTAAA 2 cut(s) 2812, 4645
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 4 cut(s) 114, 249, 1698, 1958
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 4 cut(s) 114, 249, 1698, 1958
EciI GGCGGA 3 cut(s) 2330, 2345, 3465
Ecl136II GAGCTC 1 cut(s) 4160
Eco130I CCWWGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
Eco147I AGGCCT 1 cut(s) 6315
Eco24I GRGCYC 2 cut(s) 1847, 4162
Eco31I GGTCTC 2 cut(s) 2205, 5997
Eco32I GATATC 1 cut(s) 5234
Eco47I GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
Eco47III AGCGCT 1 cut(s) 3840
Eco53kI GAGCTC 1 cut(s) 4160
Eco81I CCTNAGG 3 cut(s) 2975, 3480, 6019
Eco88I CYCGRG 1 cut(s) 713
EcoICRI GAGCTC 1 cut(s) 4160
EcoNI CCTNNNNNAGG 2 cut(s) 4689, 6018
EcoO109I RGGNCCY 6 cut(s) 61, 1843, 1844, 4086, 4309, 5813
EcoRI GAATTC 3 cut(s) 2932, 3791, 4967
EcoRII CCWGG 5 cut(s) 2426, 2644, 3111, 3909, 5621
EcoRV GATATC 1 cut(s) 5234
EcoT14I CCWWGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
EcoT22I ATGCAT 2 cut(s) 2211, 2841
EcoT38I GRGCYC 2 cut(s) 1847, 4162
ErhI CCWWGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
Esp3I CGTCTC 2 cut(s) 788, 1161
FaqI GGGAC 4 cut(s) 119, 1847, 3791, 4322
FauNDI CATATG 3 cut(s) 1186, 1838, 5980
FbaI TGATCA 1 cut(s) 3693
FblI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
FriOI GRGCYC 2 cut(s) 1847, 4162
GlaI GCGC 6 cut(s) 53, 378, 970, 1955, 3840, 5640
GsuI CTGGAG 3 cut(s) 1254, 5283, 5759
HaeII RGCGCY 3 cut(s) 55, 380, 3842
HaeIII GGCC 9 cut(s) 5, 22, 62, 91, 1845, 4088, 5166, 5814, 6315
HapII CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
HgaI GACGC 3 cut(s) 1143, 2157, 6095
HhaI GCGC 6 cut(s) 54, 379, 971, 1956, 3841, 5641
Hin1I GRCGYC 1 cut(s) 3414
Hin6I GCGC 6 cut(s) 52, 377, 969, 1954, 3839, 5639
HinP1I GCGC 6 cut(s) 52, 377, 969, 1954, 3839, 5639
HincII GTYRAC 5 cut(s) 751, 1507, 3546, 5326, 6196
HindII GTYRAC 5 cut(s) 751, 1507, 3546, 5326, 6196
HindIII AAGCTT 6 cut(s) 3059, 3121, 3803, 4932, 6032, 6268
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
Hpy99I CGWCG 3 cut(s) 90, 1071, 2151
HpyCH4IV ACGT 4 cut(s) 2012, 3414, 4267, 4660
HpySE526I ACGT 4 cut(s) 2012, 3414, 4267, 4660
Hsp92I GRCGYC 1 cut(s) 3414
HspAI GCGC 6 cut(s) 52, 377, 969, 1954, 3839, 5639
KflI GGGWCCC 1 cut(s) 4309
Ksp22I TGATCA 1 cut(s) 3693
KspAI GTTAAC 1 cut(s) 751
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 352, 718, 3817
MabI ACCWGGT 1 cut(s) 3111
MaeII ACGT 4 cut(s) 2012, 3414, 4267, 4660
MbiI CCGCTC 1 cut(s) 1386
MfeI CAATTG 3 cut(s) 1882, 2574, 3105
MflI RGATCY 5 cut(s) 2980, 3467, 4242, 5336, 5770
MhlI GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
MlyI GAGTC 6 cut(s) 158, 1502, 3742, 4495, 5756, 6260
MmeI TCCRAC 5 cut(s) 132, 1057, 1565, 5574, 5706
Mph1103I ATGCAT 2 cut(s) 2211, 2841
MroXI GAANNNNTTC 6 cut(s) 305, 2852, 5373, 5435, 5912, 6294
MspA1I CMGCKG 9 cut(s) 506, 1604, 2386, 3143, 3505, 4119, 4823, 5636, 6157
MspCI CTTAAG 1 cut(s) 2669
MspI CCGG 6 cut(s) 1062, 2163, 2277, 2782, 3655, 5108
MspR9I CCNGG 7 cut(s) 2278, 2428, 2646, 2782, 3113, 3911, 5623
MunI CAATTG 3 cut(s) 1882, 2574, 3105
Mva1269I GAATGC 1 cut(s) 5926
MvaI CCWGG 5 cut(s) 2428, 2646, 3113, 3911, 5623
NciI CCSGG 2 cut(s) 2278, 2782
NcoI CCATGG 2 cut(s) 1305, 3813
NdeI CATATG 3 cut(s) 1186, 1838, 5980
NheI GCTAGC 1 cut(s) 2462
NmeAIII GCCGAG 1 cut(s) 5425
NmuCI GTSAC 6 cut(s) 1214, 3237, 3249, 3371, 3608, 6107
NsiI ATGCAT 2 cut(s) 2211, 2841
NspV TTCGAA 2 cut(s) 2856, 5377
OliI CACNNNNGTG 1 cut(s) 506
PacI TTAATTAA 1 cut(s) 2766
PaeI GCATGC 2 cut(s) 214, 3932
PaeR7I CTCGAG 1 cut(s) 713
PagI TCATGA 2 cut(s) 937, 1794
PceI AGGCCT 1 cut(s) 6315
PciI ACATGT 1 cut(s) 1483
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 5972
PctI GAATGC 1 cut(s) 5926
PdmI GAANNNNTTC 6 cut(s) 305, 2852, 5373, 5435, 5912, 6294
PflMI CCANNNNNTGG 2 cut(s) 4516, 5507
PfoI TCCNGGA 2 cut(s) 2276, 2426
PinAI ACCGGT 2 cut(s) 1061, 2162
PleI GAGTC 6 cut(s) 157, 1502, 3742, 4495, 5756, 6259
PpsI GAGTC 6 cut(s) 157, 1502, 3742, 4495, 5756, 6259
PpuMI RGGWCCY 1 cut(s) 4309
PscI ACATGT 1 cut(s) 1483
PsiI TTATAA 2 cut(s) 588, 3213
Psp124BI GAGCTC 1 cut(s) 4162
Psp1406I AACGTT 1 cut(s) 4660
Psp5II RGGWCCY 1 cut(s) 4309
Psp6I CCWGG 5 cut(s) 2426, 2644, 3111, 3909, 5621
PspGI CCWGG 5 cut(s) 2426, 2644, 3111, 3909, 5621
PspOMI GGGCCC 1 cut(s) 1843
PspPPI RGGWCCY 1 cut(s) 4309
PspXI VCTCGAGB 1 cut(s) 713
PsrI GAACNNNNNNTAC 2 cut(s) 5025, 5057
PstI CTGCAG 4 cut(s) 1164, 1329, 2917, 4225
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 5 cut(s) 2980, 3467, 4242, 5336, 5770
PvuII CAGCTG 6 cut(s) 506, 1604, 2386, 3143, 4823, 5636
RsaI GTAC 8 cut(s) 422, 1663, 2167, 2494, 3050, 4391, 4888, 5154
RsaNI GTAC 8 cut(s) 421, 1662, 2166, 2493, 3049, 4390, 4887, 5153
SacI GAGCTC 1 cut(s) 4162
SapI GCTCTTC 1 cut(s) 249
ScaI AGTACT 1 cut(s) 4888
SchI GAGTC 6 cut(s) 158, 1502, 3742, 4495, 5756, 6260
ScrFI CCNGG 7 cut(s) 2278, 2428, 2646, 2782, 3113, 3911, 5623
SduI GDGCHC 4 cut(s) 1847, 1913, 3707, 4162
SexAI ACCWGGT 1 cut(s) 3111
SfcI CTRYAG 6 cut(s) 1160, 1325, 2174, 2416, 2913, 4221
Sfr274I CTCGAG 1 cut(s) 713
SfuI TTCGAA 2 cut(s) 2856, 5377
SinI GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
SlaI CTCGAG 1 cut(s) 713
SmiI ATTTAAAT 1 cut(s) 4645
SmlI CTYRAG 6 cut(s) 392, 713, 2669, 2938, 3639, 4341
SmoI CTYRAG 6 cut(s) 392, 713, 2669, 2938, 3639, 4341
SpeI ACTAGT 1 cut(s) 4904
SphI GCATGC 2 cut(s) 214, 3932
SseBI AGGCCT 1 cut(s) 6315
SspI AATATT 4 cut(s) 286, 1144, 5374, 5420
SstI GAGCTC 1 cut(s) 4162
StuI AGGCCT 1 cut(s) 6315
StyD4I CCNGG 7 cut(s) 2276, 2426, 2644, 2780, 3111, 3909, 5621
StyI CCWWGG 6 cut(s) 17, 1305, 3813, 4539, 5693, 6170
SwaI ATTTAAAT 1 cut(s) 4645
TaiI ACGT 4 cut(s) 2015, 3417, 4270, 4663
TatI WGTACW 3 cut(s) 3048, 4389, 4886
TauI GCSGC 1 cut(s) 4232
TseFI GTSAC 6 cut(s) 1214, 3237, 3249, 3371, 3608, 6107
Tsp45I GTSAC 6 cut(s) 1214, 3237, 3249, 3371, 3608, 6107
TspGWI ACGGA 5 cut(s) 277, 1460, 1649, 3263, 5852
Van91I CCANNNNNTGG 2 cut(s) 4516, 5507
Vha464I CTTAAG 1 cut(s) 2669
VpaK11BI GGWCC 8 cut(s) 14, 106, 1861, 2648, 3628, 3907, 4309, 4375
XagI CCTNNNNNAGG 2 cut(s) 4689, 6018
XbaI TCTAGA 4 cut(s) 1459, 2698, 4297, 5087
XcmI CCANNNNNNNNNTGG 4 cut(s) 99, 1303, 3165, 4021
XhoI CTCGAG 1 cut(s) 713
XmaJI CCTAGG 1 cut(s) 5693
XmiI GTMKAC 4 cut(s) 2326, 2419, 2664, 3277
XmnI GAANNNNTTC 6 cut(s) 305, 2852, 5373, 5435, 5912, 6294
ZraI GACGTC 1 cut(s) 3415
ZrmI AGTACT 1 cut(s) 4888
Zsp2I ATGCAT 2 cut(s) 2211, 2841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.