RLG00000032852
MYB Family

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014066
Physical Location & Seq
Forward (+)
19997630 .. 20009546
11917 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032852

Sequence Viewer

Length: 6363 bp
ATGGCTTCCAAAGGTCCAAGGCCGAAGCTCGATCACGAACCCAGACCCAAGCGCCACAAGGCCCTTGAAGTTGCGAATGAACCTCGACGGCCAAAAGCTCATTGGGACCATGTTTTAGAAGAGATGATCTGGCTGTCAAAGGACTTTGAGTCGGAGAGAAAATGGAAATTGGCTCAGGCAAAGAAGGTTGCTTTAAGAGCCAGCAAGGGCATGCTAGATCAGGCTACAAGGGGAGAAAAGAAAATGAAGGAAGAAGAGCAACGGATGAAAAAAGTTGCACTTAATATTTCAAAGGATGTGAAGAAATTCTGGCTGAAAATAGAAAAACTGGTGCTTTACAAACATCAGATGGAGCTTGATGAGAAAAAGAAAAAGGCGCTTGATAAACAGCTTGAGTTCCTTCTAGGTCAAACTGAGAGGTACTCAACAATGTTGGCAGAAAATCTTGCGGACAAGCCTGTGCAGCAATGTTCCACACAAGATCAACTGAGTATTGAAGGCACAGCTGTGGGCGAAAATGATATTAGCAAATCAGCAGAATTGAATGACGAACCTCAATCAGATACAGCAGACGGTGATGATGATTATAGTATGCAATCTGATGATGGATCAGGAGATGATGAAAACACGATTGAGGAAGATGAAGCTCTTTTTACTAAAGAAGAAAGACGGGAAGAATTGGCTGCTTTACAGAATGAAGTGGATGTTCCACTCGAGGAGCTACTCAAACAATATGCCAGAAAGAAAGTTAACACAGAAAGTAGTCAAGAAAAGGATGAAGATGTTGCAGAGATGACTTCTTCTGGGGAAGATGATGGTATATTTCCTGGAAATGGTGAAGATGACACCGAAATGACCTCAGTGGGGAAAGATCATAGTATGAGTTCAGAAAAGGGTGAAGATGGTGCAGAAGCCATGTCTGTGGGGGAAGATCATGATATGTGTCATAAAATGGGAGAAGTTGGCGCAGAAATGACCTCATTGAGTGAAGATCATGGTGAACAAAACGATCTTCTTATAGCCAGTAAGACTGATAGAAGCAGCCCAAATGTTATTACCGGTCGTCGTTGTGTTGGAAGTAATGGTGTGCCTATTTCAGAAACCCATTCATCCGAAATTAAGATTGGTGAAGCCAAAAATATTTCAGAAGCGTCTCTGCAGTCTGCCAAAGGGAATATGCCATATGATTTTGATGATGAACATGAGGATGGTGACTTCATTCTTGCTGCTGGAGATGAAAAGGATGATGAGACAACCTTATTAGAGGAGGAGGAATTGGCAAAAGCAGATACCAATCATCCCATGGATGAGATTGCATTACTGCAGAAGGAGAGTGAAATACCGTTAGAGGAATTGCTTGCAAGGTATAAAAAGGATTTGAGCAGTGATGAAGTTGCAGATGGTGAATCCGAATATGGTTCTGCTTTATCAGAGGGATTTACGGATTCTCCATCTCTAGAAGATTCTGAAGTGAAGCAACATGTTTCTATCAATGAAGATGTTGACTCTGGTGAACACCAGCCAGCTCTTGATTCTCCAACTGAAGAACATAGGGCTTCCGAAGGAGGAAGTGATAGTGAGAACAGAATTGAGGATGCAGCTGCTGCTGCAAGATCAGCACAGCCAACTGGAAACACCTTCTCCACAACAAAAGTCCGTACAAAGTTTCCCTTTCTTCTAAAGTTCCCTCTTCGTGAGTATCAGCATATTGGTTTGGATTGGCTTGTAACGATGTATGAGAAAAGGCTGAATGGGATTCTAGCCGACGAGATGGGGCTAGGGAAGACAATCATGACAATCGCTTTGCTTGCACACCTAGCCTGTGAAAAGGGCATATGGGGCCCTCATCTTATTGTGGTCCCAACCAGTGTAATGCTCAATTGGGAGACTGAGTTTCTAAAATGGTGCCCTGCTTTCAAAATCCTGACTTATTTTGGAAGTGCAAAAGAGCGAAAATTGAAGAGGCAAGGCTGGCTGAAACCAAACTCATTTCATGTCTGCATAACAACGTATAGACTGGTTATACAAGATTCAAAAGTCTTCAAGCGTAAGAAATGGAAATACTTGATTTTAGATGAAGCCCATCTGATTAAAAATTGGAAGTCGCAGAGATGGCAAACACTTTTGAACTTCAATTCCAAACGACGCATTTTGTTAACCGGTACTCCTCTACAGAATGATCTCATGGAACTTTGGTCTCTAATGCATTTCTTGATGCCCCACATATTTCAGTCTCATCAGGAGTTCAAGGATTGGTTCAGTAATCCAATATCCGGGATGGTTGAGGGGCAAGAAAAAGTAAATAAAGAAGTTCTTGATCGTCTACATAATGTCCTCCGCCCATTCATTCTCCGCCGTTTGAAGCGAGATGTGGAGAAACAGCTGCCTATGAAGCATGAGCATGTCATATACTGTAGACTTTCCAGGAGGCAACGCAACTTGTATGAAGACTTTATTGCTAGCTCAGAAACACAAGCAACTCTGGCAAGTACCAATTTTTTTGGAATGATAAGTATAATAATGCAACTTCGTAAAGTGTGCAATCATCCTGACTTATTTGAAGGTCGTCCAATTGTCAGTTCCTTTGATATGAATGGTATTTACATGCAACTGAGTTCTTCAATATGTTCAATGCTTTCGCCTGGACCATTTTCTGTTGTAGACCTTAAGGGATTGGGGTTTTTATTTACTCATCTAGATTTTTTGATGTCTTCTTGGGAGAGTGATGAAGTTAGAGCTCTTGCTACCCCATCAAGTGTAATTAAAGAGCGTGTTACCCGGATTCATCTTGAAGATATTGGTGGATTTAAACATCGTAAACATCACAAGAAGATGCATGGTATGAATATCTTCGAAGACATCCAGAAGGCACTTATGGAGGAGAGACAAAGGCAAGCAAAAGAACATGCTGCAGCTGTTGCATGGTGGAATTCCTTGAGGTGTGATAGAAAACCTATATACTCAACGAGCCTGAGGGATCTTGTTACAGTTAGACATCCTGTTGTTGATATTGCTCATCACAAGGCTAACCCTGTGTCCTATATGTACTCCTCAAAGCTTGCTGATATAGTTCTTTCACCAATAGAACGCTTCCAGAAAACAATTGACCTGGTTGAAAGCTTCGTGTTTGCAATTCCAGCTGCACGAGCTGCCCCACCTGTATGCTGGTGCAGTAAATCTGAAAGTTCTGTGTTTCTGCAACCAACTTATAAGCAGAAATGCTCTGAAGTCTTGTCACCACTTCTGTCACCATTTAGACCTGCAATTGTCCGTAGACAAGTATATTTTCCTGATAGGAGACTAATACAGTTTGACTGTGGTAAGTTGCAGCAGCTTGCTGTTTTGCTTAGGAAATTGAAGTCAGAAGGTCACAGAGCTTTGATATTCACTCAGATGACGAAGATGCTTGACGTCTTGGAGGCTTTCATCAATTTATATGGTTACACGTACATGCGTTTAGATGGATCCACTCCGCCTGAGGAGAGGCAGACTTTAATGCAGCGGTTTAACACAAACCCAAAATTTTTTCTTTTCATATTGTCAACTCGTAGTGGAGGTGTTGGTATTAACCTAGTTGGGGCAGATACAGTTATCTTTTATGATAGTGACTGGAATCCTGCTATGGACCAACAAGCTCAAGATCGTTGCCACCGGATTGGACAGACACGAGAAGTTCATATTTATCGATTGATCAGTGAGAGCACCATTGAAGAAAACATTTTGAAGAAAGCAAATCAGAAGCGGACTCTTGACAATCTAGTTATACAAAGTGGGGACTATAACACTGAATTCTTCAAAAAGCTTGATCCCATGGAGCTGTTTTCTGGTCATAGAGCGCTTCCCATTAAGAACATGCAAAAGGAGAAAAATCACAACACAACTGAAGTTTCTCTATCTAATGTGGACCTGGAAGCTGCACTAAAGCATGCAGAAGATGAAGCAGATTATATGGCATTAAAGAAAGTAGAGCAGGAGGAAGCTGTGGACAACCAAGAATTTACTGAGGAAGCCATTGTGAGATTGGAAGATGATGAATTAGTGAATGAGGACGATATGAAGGTTGATGATCCCACAGATCAGGGGGCTTTGATGATGATGTCTTCAAACAAAGACAGCGGAATGATGTTGAATGGGAGTGATCCTAATGAAGAAAGAGCTCTCACTGGTGCTTGTAGGGAAGATGATGTGGACATGATGGCTGATGTCAAACAGATGGCTGCAGCTGCTGCTGCTGCAGGACAAGAGATCTCATCCTTTGGAAATCAGTTACGTCCGATAGATCATTATGCAATTCGTTTTCTAGAATTGTGGGACCCAATAATAGACAAGACAGCCGCAGAATCTCAAGTTAGATTTGAGGAGAGAGAGTGGGAACTGGACCGCATAGAAAAGTACAAGGAGGAAATGGAAGCTGAGATTGATGAAGATGAAGAACCTCTAGTATATGAAACATGGGATGCTGAATTTGCAACTGAGGCATATCGGCAGCAGGTTGAAGCCTTGACTCAACACCAGTTGATGGAGGAACTGGAATATGAAGCCAAGGAGAAGGAAGATGAAGCAGATGAAAATCTTGATTCTCTGAGGTATGGGATGCCAAGTGATCCTAAACCCAAGCATAAGAAGAAATCGAAGAAAGCAAAATTTAAATCTCTGAAGAAACGCTCTCTAGCTTCTGAGCTGGAACCTGTTAAAGGAGAGCTGCAAGTGGAACCTATGTTCATAGATGAAGATTATCTCTCTAATGATGTGTTTTCCTATTCGGATATTGAATCACCTCATTCAAGTGTGCAGAAAAAACGCAAGAAGGCTGAATCTAAACCAGCTGGTGAAGATAAGATTTCAAAGAAGAAGTCTAAGAAACTGAAGAAGTCTCATCTTGAAATCAGTAGTTCAGAGTTTGATACTAGTGTCTCCTCAGTGCAGCGTGTTGAAGCTTCAGAGTTAAAACCATGTGAGAGTGTGGTTGAATTCGAGCATAAACCAATTAGTAGAACCAAAATGGGAGGGAAAATATCCATCACTGCCATGCCTGTAAAGCGAGTTCTAATGATAAAACCAGAGAAGCTAAAGAAGGGAAACATTTGGTCTAGAGATTGTATTCCATCACCAGATTTTTGGTTATCACAGGAGGATGCAATTTTATGTGCTGTGGTACATGAGTATGGCCCTTATTGGAGCTTGGTGAGTGAGACGCTTTATGGTATGACTGCTGGTGGGTTTTATAGGGGGAGATATCGCCATCCAATTCATTGTTGTGAGAGGTTTAGGGAACTTATCCAAAGATATGTTTTCTCCGCTCCAGACAATCCTAATTATGAAAAGGTCAACAATATAGGATCTGGAAAAGCTCTTTTAAGAGTAACAGAGGAAAATATTCGGATGTTATTAAATGTTGCTGCCGAGCAGCCCAATACAGAATATTTGATTCAGAAACACTTCATTGCCCTGCTATCCTCTGTCTGGAAGATGGCATCTCACAAAGACGGGAGACAAAATCTCCCATCTTCTGGGAATGGTGTATATTTTGGCGGAAATTTTTTCAGTTCTTCCAGCCAAATTTCTCGGACTTCAGTGAAGGAAAACACAGCAACCATGAAATTTACCAATTTTGGACAGGGTGCCAGGTTGGTAGCAGCTGCACTTAATGATGCCAGCAGCAGGCGAGGGGATGAGTGTGTTATTTCTCCCAACCCTAGGAAAAAATCGTCAACCGATGCTGAACGGTTGGATATAACTCTCGAGTTTCAGGCACAAAAGGATGACTCCACAGATCCATTTCCATCTGTTGTCAATTTATCAATATCTGATATAGGCCCTCCATCAGAAAATAAGGCAATGGAACCGTATCTTCCCAGAGATTCCTGTAATGACTATTGCAAAAGCTCCAGTGATGCAAATGTGGCTGAGAACCGTTTCAGGAATGCAACAAGAGCTTGCGATGAAGATACTATGGGGTGGGCTTCATCGACATTCCCAACATTTGATTTCAGGTCCCGATCAGTGTCAAAGTTACCTTCCTCAGGAAAGCACAAGCTTGTCTTCTCCGATCCAGTTAGACCTTCAAAATCCAAGTTCAGAAAAAATGCAGTAGAGCATGGTGAGATGCGTCACTTTCTGGCTGAGCATGTATTCCCACCCTTGCCAATAGCTGCACCGCTGAATCCCAGTCCAAGGTTCGACCTGAACCTACCTGTCAACGAGGATATTGAGTCTGGTGATCTGGGAAGCACTTCCCATTCTCAGGTGGTTGAGTCTTCATTTGAAGAAAGCTTTGGAGTTCTCCCACATGAATATGTTCCTGAATTGCTTTCAGGCCTTGATGACTGTTCATTATTGCCAGAATATGATGACATTGCCTAA

Protein Analysis

2121

Amino Acids

240.88

Weight (kDa)

5.35

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSA PF07529 27 - 93 3.6e-14 HSA domain
ResIII PF04851 562 - 723 2.4e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 567 - 852 2.4e-89 SNF2-related domain
DEAD PF00270 584 - 724 7.8e-08 DEAD/DEAH box helicase
Helicase_C PF00271 1108 - 1220 2.3e-26 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12810 AT3G12810
fragaria_vesca FvH4_3g15170
malus_domestica MD10G1206600.v1.1
prunus_persica Prupe.4G135700_v2.0.a1 Prupe.4G135700_v2.0.a1
pyrus_communis pycom10g17640
rosa_chinensis RchiOBHm_Chr5g0025621
rosa_laevigata RLG00000032852
rosa_multiflora Rmu_sc0004107.1_g000018
rosa_roxburghii Rroxscaffold_1G00054140
rosa_rugosa Rorug05G0087200
rosa_samantha Rh5AG179000 Rh5BG178100 Rh5CG195700 Rh5DG177300
rosa_wichuraiana Rw5G016250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3210
AatII GACGTC 1 cut(s) 3414
Acc36I ACCTGC 2 cut(s) 3268, 4480
AccB1I GGYRCC 2 cut(s) 1905, 5621
AccB7I CCANNNNNTGG 2 cut(s) 4519, 5510
AccBSI CCGCTC 1 cut(s) 5300
AccI GTMKAC 4 cut(s) 2323, 2416, 2661, 3274
AcoI YGGCCR 1 cut(s) 89
AcuI CTGAAG 8 cut(s) 1488, 1563, 3246, 3903, 4676, 4886, 4923, 5556
AcyI GRCGYC 1 cut(s) 3411
AfaI GTAC 8 cut(s) 422, 1660, 2164, 2491, 3047, 3449, 4394, 5157
AfeI AGCGCT 1 cut(s) 3837
AflII CTTAAG 1 cut(s) 2666
AflIII ACRYGT 2 cut(s) 1480, 3444
AgeI ACCGGT 2 cut(s) 1058, 2159
AhdI GACNNNNNGTC 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 4907
AjnI CCWGG 6 cut(s) 826, 2423, 2641, 3108, 3906, 5624
AjuI GAANNNNNNNTTGG 6 cut(s) 1107, 1139, 2563, 2595, 6258, 6290
AleI CACNNNNGTG 1 cut(s) 506
AloI GAACNNNNNNTCC 4 cut(s) 2120, 2152, 4703, 4735
Alw21I GWGCWC 3 cut(s) 2740, 3704, 4159
AlwNI CAGNNNCTG 2 cut(s) 1604, 4226
Ama87I CYCGRG 2 cut(s) 713, 5741
Aor51HI AGCGCT 1 cut(s) 3837
AoxI GGCC 7 cut(s) 20, 60, 89, 1840, 5167, 5815, 6316
ApaI GGGCCC 1 cut(s) 1844
ArsI GACNNNNNNTTYG 4 cut(s) 1786, 1818, 4838, 4870
AsiGI ACCGGT 2 cut(s) 1058, 2159
Asp700I GAANNNNTTC 7 cut(s) 305, 2849, 5376, 5438, 5915, 6232, 6297
AspA2I CCTAGG 1 cut(s) 5696
AspLEI GCGC 4 cut(s) 54, 379, 968, 3838
AsuC2I CCSGG 2 cut(s) 2275, 2779
AsuII TTCGAA 1 cut(s) 2853
AsuNHI GCTAGC 1 cut(s) 2459
AvaI CYCGRG 2 cut(s) 713, 5741
AvaII GGWCC 9 cut(s) 14, 106, 1858, 2645, 3625, 3904, 4312, 4378, 5994
AvrII CCTAGG 1 cut(s) 5696
AxyI CCTNAGG 3 cut(s) 2972, 3477, 6022
BaeGI GKGCMC 2 cut(s) 1844, 1910
BaeI ACNNNNGTAYC 4 cut(s) 4896, 4929, 5147, 5180
BamHI GGATCC 1 cut(s) 3464
BanI GGYRCC 2 cut(s) 1905, 5621
BanII GRGCYC 3 cut(s) 1844, 2740, 4159
BauI CACGAG 2 cut(s) 3144, 3666
BbsI GAAGAC 8 cut(s) 1790, 2032, 2454, 2703, 2862, 4092, 6034, 6249
Bbv12I GWGCWC 3 cut(s) 2740, 3704, 4159
BceAI ACGGC 2 cut(s) 104, 2340
BciT130I CCWGG 6 cut(s) 828, 2425, 2643, 3110, 3908, 5626
BclI TGATCA 1 cut(s) 3690
BcnI CCSGG 2 cut(s) 2275, 2779
BcuI ACTAGT 1 cut(s) 4907
BfmI CTRYAG 7 cut(s) 1157, 1322, 2171, 2413, 2910, 4218, 4233
BfoI RGCGCY 3 cut(s) 55, 380, 3839
BfrI CTTAAG 1 cut(s) 2666
BfuAI ACCTGC 2 cut(s) 3268, 4480
BglII AGATCT 1 cut(s) 4245
BlnI CCTAGG 1 cut(s) 5696
BlpI GCTNAGC 1 cut(s) 6123
Bme1390I CCNGG 8 cut(s) 828, 2275, 2425, 2643, 2779, 3110, 3908, 5626
Bme18I GGWCC 9 cut(s) 14, 106, 1858, 2645, 3625, 3904, 4312, 4378, 5994
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 2 cut(s) 713, 5741
BmrFI CCNGG 8 cut(s) 828, 2275, 2425, 2643, 2779, 3110, 3908, 5626
BmrI ACTGGG 1 cut(s) 6162
BmtI GCTAGC 1 cut(s) 2463
BmuI ACTGGG 1 cut(s) 6162
BpiI GAAGAC 8 cut(s) 1790, 2032, 2454, 2703, 2862, 4092, 6034, 6249
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 3 cut(s) 1251, 5286, 5872
Bpu10I CCTNAGC 2 cut(s) 174, 3347
Bpu1102I GCTNAGC 1 cut(s) 6123
Bpu14I TTCGAA 1 cut(s) 2853
BpuEI CTTGAG 4 cut(s) 413, 2956, 3621, 4329
BpuMI CCSGG 2 cut(s) 2275, 2779
Bsa29I ATCGAT 1 cut(s) 3685
BsaAI YACGTR 1 cut(s) 3447
BsaBI GATNNNNATC 2 cut(s) 1293, 4569
BsaHI GRCGYC 1 cut(s) 3411
BsaI GGTCTC 1 cut(s) 2202
BsaJI CCNNGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
BsaWI WCCGGW 3 cut(s) 1058, 2159, 3651
Bse118I RCCGGY 2 cut(s) 1058, 2159
Bse21I CCTNAGG 3 cut(s) 2972, 3477, 6022
Bse3DI GCAATG 4 cut(s) 473, 5442, 5844, 6354
Bse8I GATNNNNATC 2 cut(s) 1293, 4569
BseBI CCWGG 6 cut(s) 828, 2425, 2643, 3110, 3908, 5626
BseCI ATCGAT 1 cut(s) 3685
BseDI CCNNGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
BseJI GATNNNNATC 2 cut(s) 1293, 4569
BseMI GCAATG 4 cut(s) 473, 5442, 5844, 6354
BseRI GAGGAG 9 cut(s) 731, 1280, 1283, 2157, 2894, 3040, 3494, 4373, 4906
BseSI GKGCMC 2 cut(s) 1844, 1910
BsgI GTGCAG 9 cut(s) 482, 927, 3126, 3190, 3900, 4811, 4943, 5625, 6138
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 7 cut(s) 22, 62, 91, 1842, 5169, 5817, 6318
BshNI GGYRCC 2 cut(s) 1905, 5621
BshTI ACCGGT 2 cut(s) 1058, 2159
BshVI ATCGAT 1 cut(s) 3685
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 3 cut(s) 2740, 3704, 4159
BsiHKCI CYCGRG 2 cut(s) 713, 5741
BsiSI CCGG 5 cut(s) 1059, 2160, 2274, 2779, 3652
BslFI GGGAC 5 cut(s) 119, 1844, 3788, 4325, 5980
BsmBI CGTCTC 2 cut(s) 1158, 5186
BsmFI GGGAC 5 cut(s) 119, 1844, 3788, 4325, 5980
BsmI GAATGC 1 cut(s) 5929
BsnI GGCC 7 cut(s) 22, 62, 91, 1842, 5169, 5817, 6318
Bso31I GGTCTC 1 cut(s) 2202
BsoBI CYCGRG 2 cut(s) 713, 5741
Bsp119I TTCGAA 1 cut(s) 2853
Bsp120I GGGCCC 1 cut(s) 1840
Bsp1286I GDGCHC 5 cut(s) 1844, 1910, 2740, 3704, 4159
Bsp1720I GCTNAGC 1 cut(s) 6123
Bsp19I CCATGG 2 cut(s) 1302, 3810
BspANI GGCC 7 cut(s) 22, 62, 91, 1842, 5169, 5817, 6318
BspDI ATCGAT 1 cut(s) 3685
BspHI TCATGA 2 cut(s) 934, 1791
BspMAI CTGCAG 5 cut(s) 1161, 1326, 2914, 4222, 4237
BspMI ACCTGC 2 cut(s) 3268, 4480
BspOI GCTAGC 1 cut(s) 2463
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 2853
BspT107I GGYRCC 2 cut(s) 1905, 5621
BspTI CTTAAG 1 cut(s) 2666
BspTNI GGTCTC 1 cut(s) 2202
BsrBI CCGCTC 1 cut(s) 5300
BsrDI GCAATG 4 cut(s) 473, 5442, 5844, 6354
BsrFI RCCGGY 2 cut(s) 1058, 2159
BssAI RCCGGY 2 cut(s) 1058, 2159
BssECI CCNNGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
BssNI GRCGYC 1 cut(s) 3411
BssSI CACGAG 2 cut(s) 3144, 3666
BssT1I CCWWGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
Bst2BI CACGAG 2 cut(s) 3144, 3666
Bst2UI CCWGG 6 cut(s) 828, 2425, 2643, 3110, 3908, 5626
Bst6I CTCTTC 4 cut(s) 114, 249, 1695, 1955
BstACI GRCGYC 1 cut(s) 3411
BstAFI CTTAAG 1 cut(s) 2666
BstAPI GCANNNNNTGC 4 cut(s) 1604, 2918, 3149, 4226
BstBAI YACGTR 1 cut(s) 3447
BstBI TTCGAA 1 cut(s) 2853
BstDSI CCRYGG 2 cut(s) 1302, 3810
BstENI CCTNNNNNAGG 2 cut(s) 4692, 6021
BstH2I RGCGCY 3 cut(s) 55, 380, 3839
BstHHI GCGC 4 cut(s) 54, 379, 968, 3838
BstMCI CGRYCG 1 cut(s) 1063
BstNI CCWGG 6 cut(s) 828, 2425, 2643, 3110, 3908, 5626
BstNSI RCATGY 9 cut(s) 214, 1484, 2405, 2608, 2909, 3454, 3856, 3929, 6131
BstSCI CCNGG 8 cut(s) 826, 2273, 2423, 2641, 2777, 3108, 3906, 5624
BstSFI CTRYAG 7 cut(s) 1157, 1322, 2171, 2413, 2910, 4218, 4233
BstSLI GKGCMC 2 cut(s) 1844, 1910
BstV2I GAAGAC 8 cut(s) 1790, 2032, 2454, 2703, 2862, 4092, 6034, 6249
BstX2I RGATCY 5 cut(s) 2977, 3464, 4245, 5339, 5773
BstXI CCANNNNNNTGG 3 cut(s) 922, 3656, 5118
BstYI RGATCY 5 cut(s) 2977, 3464, 4245, 5339, 5773
Bsu15I ATCGAT 1 cut(s) 3685
Bsu36I CCTNAGG 3 cut(s) 2972, 3477, 6022
BsuRI GGCC 7 cut(s) 22, 62, 91, 1842, 5169, 5817, 6318
BsuTUI ATCGAT 1 cut(s) 3685
BtgI CCRYGG 2 cut(s) 1302, 3810
BtgZI GCGATG 1 cut(s) 5955
BtsI GCAGTG 2 cut(s) 1390, 5022
BveI ACCTGC 2 cut(s) 3268, 4480
CaiI CAGNNNCTG 2 cut(s) 1604, 4226
CciI TCATGA 2 cut(s) 934, 1791
CfoI GCGC 4 cut(s) 54, 379, 968, 3838
Cfr10I RCCGGY 2 cut(s) 1058, 2159
ClaI ATCGAT 1 cut(s) 3685
CseI GACGC 4 cut(s) 1140, 2154, 5203, 6098
CsiI ACCWGGT 1 cut(s) 3108
Csp6I GTAC 8 cut(s) 421, 1659, 2163, 2490, 3046, 3448, 4393, 5156
CspAI ACCGGT 2 cut(s) 1058, 2159
CviQI GTAC 8 cut(s) 421, 1659, 2163, 2490, 3046, 3448, 4393, 5156
DraI TTTAAA 2 cut(s) 2809, 4648
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 4 cut(s) 114, 249, 1695, 1955
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 4 cut(s) 114, 249, 1695, 1955
EciI GGCGGA 4 cut(s) 2327, 2342, 3462, 5547
Ecl136II GAGCTC 2 cut(s) 2738, 4157
Eco130I CCWWGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
Eco147I AGGCCT 1 cut(s) 6318
Eco24I GRGCYC 3 cut(s) 1844, 2740, 4159
Eco31I GGTCTC 1 cut(s) 2202
Eco32I GATATC 1 cut(s) 5237
Eco47I GGWCC 9 cut(s) 14, 106, 1858, 2645, 3625, 3904, 4312, 4378, 5994
Eco47III AGCGCT 1 cut(s) 3837
Eco53kI GAGCTC 2 cut(s) 2738, 4157
Eco57I CTGAAG 8 cut(s) 1488, 1563, 3246, 3903, 4676, 4886, 4923, 5556
Eco81I CCTNAGG 3 cut(s) 2972, 3477, 6022
Eco88I CYCGRG 2 cut(s) 713, 5741
EcoICRI GAGCTC 2 cut(s) 2738, 4157
EcoNI CCTNNNNNAGG 2 cut(s) 4692, 6021
EcoO109I RGGNCCY 6 cut(s) 61, 1840, 1841, 4312, 5816, 5994
EcoRI GAATTC 3 cut(s) 2929, 3788, 4970
EcoRII CCWGG 6 cut(s) 826, 2423, 2641, 3108, 3906, 5624
EcoRV GATATC 1 cut(s) 5237
EcoT14I CCWWGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
EcoT22I ATGCAT 2 cut(s) 2208, 2838
EcoT38I GRGCYC 3 cut(s) 1844, 2740, 4159
ErhI CCWWGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
Esp3I CGTCTC 2 cut(s) 1158, 5186
FalI AAGNNNNNCTT 8 cut(s) 264, 296, 1655, 1687, 2298, 2330, 6026, 6058
FaqI GGGAC 5 cut(s) 119, 1844, 3788, 4325, 5980
FauNDI CATATG 2 cut(s) 1183, 1835
FbaI TGATCA 1 cut(s) 3690
FblI GTMKAC 4 cut(s) 2323, 2416, 2661, 3274
FriOI GRGCYC 3 cut(s) 1844, 2740, 4159
GlaI GCGC 4 cut(s) 53, 378, 967, 3837
GsuI CTGGAG 3 cut(s) 1251, 5286, 5872
HaeII RGCGCY 3 cut(s) 55, 380, 3839
HaeIII GGCC 7 cut(s) 22, 62, 91, 1842, 5169, 5817, 6318
HapII CCGG 5 cut(s) 1059, 2160, 2274, 2779, 3652
HgaI GACGC 4 cut(s) 1140, 2154, 5203, 6098
HhaI GCGC 4 cut(s) 54, 379, 968, 3838
Hin1I GRCGYC 1 cut(s) 3411
Hin6I GCGC 4 cut(s) 52, 377, 966, 3836
HinP1I GCGC 4 cut(s) 52, 377, 966, 3836
HincII GTYRAC 7 cut(s) 751, 1504, 2157, 3543, 5329, 5712, 6199
HindII GTYRAC 7 cut(s) 751, 1504, 2157, 3543, 5329, 5712, 6199
HindIII AAGCTT 6 cut(s) 3056, 3118, 3800, 4935, 6035, 6271
HpaI GTTAAC 2 cut(s) 751, 2157
HpaII CCGG 5 cut(s) 1059, 2160, 2274, 2779, 3652
Hpy99I CGWCG 4 cut(s) 90, 1068, 1769, 2148
HpyCH4IV ACGT 4 cut(s) 2009, 3411, 3446, 4270
HpySE526I ACGT 4 cut(s) 2009, 3411, 3446, 4270
Hsp92I GRCGYC 1 cut(s) 3411
HspAI GCGC 4 cut(s) 52, 377, 966, 3836
KflI GGGWCCC 1 cut(s) 4312
Ksp22I TGATCA 1 cut(s) 3690
KspAI GTTAAC 2 cut(s) 751, 2157
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 6 cut(s) 352, 718, 3814, 5178, 5305, 5891
MabI ACCWGGT 1 cut(s) 3108
MaeII ACGT 4 cut(s) 2009, 3411, 3446, 4270
MbiI CCGCTC 1 cut(s) 5300
MfeI CAATTG 4 cut(s) 1879, 2571, 3102, 3264
MflI RGATCY 5 cut(s) 2977, 3464, 4245, 5339, 5773
MhlI GDGCHC 5 cut(s) 1844, 1910, 2740, 3704, 4159
MlyI GAGTC 7 cut(s) 158, 1499, 3739, 4498, 5759, 6221, 6263
MmeI TCCRAC 4 cut(s) 132, 1054, 1562, 5709
Mph1103I ATGCAT 2 cut(s) 2208, 2838
MroXI GAANNNNTTC 7 cut(s) 305, 2849, 5376, 5438, 5915, 6232, 6297
MspCI CTTAAG 1 cut(s) 2666
MspI CCGG 5 cut(s) 1059, 2160, 2274, 2779, 3652
MspR9I CCNGG 8 cut(s) 828, 2275, 2425, 2643, 2779, 3110, 3908, 5626
MunI CAATTG 4 cut(s) 1879, 2571, 3102, 3264
Mva1269I GAATGC 1 cut(s) 5929
MvaI CCWGG 6 cut(s) 828, 2425, 2643, 3110, 3908, 5626
NciI CCSGG 2 cut(s) 2275, 2779
NcoI CCATGG 2 cut(s) 1302, 3810
NdeI CATATG 2 cut(s) 1183, 1835
NheI GCTAGC 1 cut(s) 2459
NmeAIII GCCGAG 1 cut(s) 5428
NmuCI GTSAC 6 cut(s) 1211, 3234, 3246, 3368, 3605, 6110
NsiI ATGCAT 2 cut(s) 2208, 2838
NspI RCATGY 9 cut(s) 214, 1484, 2405, 2608, 2909, 3454, 3856, 3929, 6131
NspV TTCGAA 1 cut(s) 2853
OliI CACNNNNGTG 1 cut(s) 506
PaeI GCATGC 2 cut(s) 214, 3929
PaeR7I CTCGAG 2 cut(s) 713, 5741
PagI TCATGA 2 cut(s) 934, 1791
PceI AGGCCT 1 cut(s) 6318
PciI ACATGT 1 cut(s) 1480
PciSI GCTCTTC 1 cut(s) 249
PctI GAATGC 1 cut(s) 5929
PdmI GAANNNNTTC 7 cut(s) 305, 2849, 5376, 5438, 5915, 6232, 6297
PflMI CCANNNNNTGG 2 cut(s) 4519, 5510
PfoI TCCNGGA 3 cut(s) 826, 2273, 2423
PinAI ACCGGT 2 cut(s) 1058, 2159
PleI GAGTC 7 cut(s) 157, 1499, 3739, 4498, 5759, 6220, 6262
PpsI GAGTC 7 cut(s) 157, 1499, 3739, 4498, 5759, 6220, 6262
Ppu21I YACGTR 1 cut(s) 3447
PpuMI RGGWCCY 2 cut(s) 4312, 5994
PscI ACATGT 1 cut(s) 1480
PsiI TTATAA 1 cut(s) 3210
Psp124BI GAGCTC 2 cut(s) 2740, 4159
Psp5II RGGWCCY 2 cut(s) 4312, 5994
Psp6I CCWGG 6 cut(s) 826, 2423, 2641, 3108, 3906, 5624
PspGI CCWGG 6 cut(s) 826, 2423, 2641, 3108, 3906, 5624
PspOMI GGGCCC 1 cut(s) 1840
PspPPI RGGWCCY 2 cut(s) 4312, 5994
PspXI VCTCGAGB 1 cut(s) 713
PsrI GAACNNNNNNTAC 2 cut(s) 5028, 5060
PstI CTGCAG 5 cut(s) 1161, 1326, 2914, 4222, 4237
PstNI CAGNNNCTG 2 cut(s) 1604, 4226
PsuI RGATCY 5 cut(s) 2977, 3464, 4245, 5339, 5773
PvuII CAGCTG 8 cut(s) 506, 1601, 2383, 2915, 3140, 4223, 4826, 5639
RsaI GTAC 8 cut(s) 422, 1660, 2164, 2491, 3047, 3449, 4394, 5157
RsaNI GTAC 8 cut(s) 421, 1659, 2163, 2490, 3046, 3448, 4393, 5156
SacI GAGCTC 2 cut(s) 2740, 4159
SapI GCTCTTC 1 cut(s) 249
SchI GAGTC 7 cut(s) 158, 1499, 3739, 4498, 5759, 6221, 6263
ScrFI CCNGG 8 cut(s) 828, 2275, 2425, 2643, 2779, 3110, 3908, 5626
SduI GDGCHC 5 cut(s) 1844, 1910, 2740, 3704, 4159
SexAI ACCWGGT 1 cut(s) 3108
SfcI CTRYAG 7 cut(s) 1157, 1322, 2171, 2413, 2910, 4218, 4233
Sfr274I CTCGAG 2 cut(s) 713, 5741
SfuI TTCGAA 1 cut(s) 2853
SinI GGWCC 9 cut(s) 14, 106, 1858, 2645, 3625, 3904, 4312, 4378, 5994
SlaI CTCGAG 2 cut(s) 713, 5741
SmiI ATTTAAAT 1 cut(s) 4648
SmlI CTYRAG 7 cut(s) 392, 713, 2666, 2935, 3636, 4344, 5741
SmoI CTYRAG 7 cut(s) 392, 713, 2666, 2935, 3636, 4344, 5741
SpeI ACTAGT 1 cut(s) 4907
SphI GCATGC 2 cut(s) 214, 3929
SseBI AGGCCT 1 cut(s) 6318
SspI AATATT 4 cut(s) 286, 1141, 5377, 5423
SstI GAGCTC 2 cut(s) 2740, 4159
StuI AGGCCT 1 cut(s) 6318
StyD4I CCNGG 8 cut(s) 826, 2273, 2423, 2641, 2777, 3108, 3906, 5624
StyI CCWWGG 6 cut(s) 17, 1302, 3810, 4542, 5696, 6173
SwaI ATTTAAAT 1 cut(s) 4648
TaiI ACGT 4 cut(s) 2012, 3414, 3449, 4273
TatI WGTACW 2 cut(s) 3045, 4392
TauI GCSGC 1 cut(s) 4337
TseFI GTSAC 6 cut(s) 1211, 3234, 3246, 3368, 3605, 6110
Tsp45I GTSAC 6 cut(s) 1211, 3234, 3246, 3368, 3605, 6110
TspGWI ACGGA 4 cut(s) 277, 1457, 1646, 3260
Van91I CCANNNNNTGG 2 cut(s) 4519, 5510
Vha464I CTTAAG 1 cut(s) 2666
VpaK11BI GGWCC 9 cut(s) 14, 106, 1858, 2645, 3625, 3904, 4312, 4378, 5994
XagI CCTNNNNNAGG 2 cut(s) 4692, 6021
XbaI TCTAGA 4 cut(s) 1456, 2695, 4300, 5090
XceI RCATGY 9 cut(s) 214, 1484, 2405, 2608, 2909, 3454, 3856, 3929, 6131
XcmI CCANNNNNNNNNTGG 4 cut(s) 99, 1300, 3162, 4018
XhoI CTCGAG 2 cut(s) 713, 5741
XmaJI CCTAGG 1 cut(s) 5696
XmiI GTMKAC 4 cut(s) 2323, 2416, 2661, 3274
XmnI GAANNNNTTC 7 cut(s) 305, 2849, 5376, 5438, 5915, 6232, 6297
ZraI GACGTC 1 cut(s) 3412
Zsp2I ATGCAT 2 cut(s) 2208, 2838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.