AT3G13990

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005772
Physical Location & Seq
Reverse (-)
4625383 .. 4630613
5231 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13990.2

Sequence Viewer

Length: 2547 bp
ATGGTGACTGGGTCCAGAACTAGCGGTAATCGTGGGGTAGGACTCGATGATGAAGCGAAAAAGATGATTCAATCGATTAAGGAAGTTGTGGATAGTCACTCCGATGCTGATATTTACACTGCCTTAAAAGAAGCTAACATGGATGCTAACGAGGCTGTGGAGAAGCTGATTCATCAAGATCCATTTCACGAGGTGAAGAGAAAAAGAGACAGGAAAAAAGAGGATACAGTATTTGTTGAGCCTGCCAACATAAAGAAACCTCTTGAAAATGTTACCAGTGAAGTGAAAGTTCGTACACAGCCTGAACATAATGTTCGGAGAGGAGGCTACAGTAGGAATTTCTTCCCCAGGAACGCTGCTCAACGAAATGCATCTCCTAGAAATCCTGCTACTGGATCCAACAAAGAGTTTCGCGTTGTTAGGGACAACAGGTCTAATCCAAATGTTGATGAAGAGTTGAAGCATTCTTCTGCCCAGTCCTCTGGATCAAATATTAACAAAGTGGTTGCCACTGTGAACAAATTGGGCTCAAGAGGTGGTTTGGGCAATCGTAACTCTTCTGGGGCTCAAGATTCAACTGACGAGTGTAATGCTCCAGCAGATGCCAGGCTCAGACAGGCTGAAATTCGTCCTTTGCATTGTCCTGTCAATAAAGAACTCGGTGGAGTTACTTTGCCATCAACCAATTCAGTTCTTGGTGTATATTCGTCTTCCAAAGATCCAGTTCATGTACCTTCTCCTGTTTCTAGATCATCACCCGTGGGAGCTATTAAACGTGAAGTAAGGGGTGGAGGTTTAGGTGGAAAACCTTCTGAAAATGTTGGTAAAGATCCATCTGTGCCCTCTTTTTCAGGGTCCTCTATTAGAAAAAATGGACCTCCAAATGCACATCGACCATCTTCCCCAACTTCAAAGATTGATCACAGTCGAACTACTGCACGTGAGTCTGTTATGCCCAGTGGAGTGGAAAAGAATAGGTCATATTTGAACAGACAGCGTGGGAATAGAGGAAGCCAATATGCTAGAACACAACAAGTTGGTGGCCATACAAAGGGAGCCTCACAGAACAAGGAGTGGAAGCCTAAATCTAATCAGAAACCTGTTGGACATAACCCTGGAGTAATTGGAACACCTACAAAGTCTCAAGCTCGTCGTCCTGCTGATAATTCCATAAATGTGGAAACAGAAGCCGTCAAGTTGCAGAATAAGCTTTCACATGTTCATATAAGTGAAAGTCAGAATGTCATCATCGCAGATCATATTCGTGTCCCGGAGACTGACCGCTGTCAGCTAACTTTTGGTAGCTTTGTTCAAGAGTTTAATTCCTCAAGGAATTCAGAATCTGCTTTCGAAGAATCACACTCTTCAGAAGAAGTTAGAGAATCTGATCGTAGTTCGCCAGTTACTTGTCCTGAGGCTTCAGCAGATGGTCCGGGCGTTCTGCCTATCACTATTGATGATCATGTCAGAGTTTCTAGATCTGACTCACCCGTCTCGGCAGTATCTGAGCCACAGTTACCCGAACAGAAAGAAACTCATAGATCTGACAATTTGGATGCGTACTCGGGGATTGAATTACTCAATAGGAATGGTCCACCTTATACTCCTTTGGAGTTTGAACAGCAGCAAGATCCTCCGGAGTTGCAAAAATTTTCTCAGGCTTATGACAATCATGGTAGTTATGAATTTCCGTACTTTAGTCCTGCAATGGATGAAACTGTACGAGTTCAAGGATTACCATCTCCACACGAGCAGGGATTAAGTGCTCACATGTTAAATAATGCCCCACCATCCACTATTCCCATGTTACAGCAACAACAGCAGGCATCAATGCAACAGATGTATCCGCAGGTCCATGTTTCGCATTTTCCCAATCTCATGCCTTATCGTCAATTCCTTTCCCCAGTCTATGTTCCCCAAATGCCCATGCCAGGCTACTCAGGTAACCCAGCAGCATATGCACACCCTTCAAATGGCAATAGCTATGTGCTGATGCCTGGAGGCGGTTCTCATCTCGGTTCAAATGGTGTCAAGTATGGAATCCATCAGCAATATAAACCAGTTCCAACTGGTGGACCTGCGGGTTTTGGGACTTACAACAATCCTAATGGGTATCCAACTAATCCTCCCAATGTAGTTGGAAACGCTACGGGACTTGAAGATCCATCTCGCATGATAAATAAACATGGAAATATTTACGTTCCAAATCCTCAGGCTGAGACTTCTGAGATATGGATGCAAAATCCAAGAGATCTTTCAAGCCTTCAGTCTCCATATTACAACGTAGCTGGACAATCGCCTCATGGTGCTTATTTATCATCCCATACAGCACACCAATCGTTCAACCCAACTGCGCAATCATCTCACATGCAGTTCCAGGGTCTCTTCCACCCGTCACAGCCTGGTACGATGGCGAATCCGCACCACATGGGACCTGGCCTCAGTGGTAATGTTGGAGTCGGTGTCGTTCCCTCTCCTCCTCCGTCTCAAATTGGTACCTATCAGCAATCACAACTTGGTCATCCGAATTGGCCATCAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

848

Amino Acids

92.3

Weight (kDa)

8.67

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 17 - 74 1.2e-28 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1490
Acc16I TGCGCA 1 cut(s) 2355
Acc36I ACCTGC 2 cut(s) 1840, 2086
Acc65I GGTACC 1 cut(s) 2495
AccB1I GGYRCC 1 cut(s) 2495
AccB7I CCANNNNNTGG 1 cut(s) 2072
AccII CGCG 1 cut(s) 414
AccIII TCCGGA 1 cut(s) 1636
AciI CCGC 6 cut(s) 24, 1282, 1847, 2004, 2081, 2420
AclWI GGATC 8 cut(s) 173, 390, 403, 493, 713, 824, 1625, 2156
AcoI YGGCCR 2 cut(s) 1042, 2531
AcsI RAATTY 5 cut(s) 337, 624, 1333, 1649, 1685
AcuI CTGAAG 3 cut(s) 1350, 1404, 2249
AcvI CACGTG 1 cut(s) 941
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 7 cut(s) 295, 732, 1562, 1694, 1722, 2407, 2497
AfiI CCNNNNNNNGG 6 cut(s) 392, 1051, 1931, 1973, 2003, 2072
AflIII ACRYGT 2 cut(s) 1216, 1770
AhdI GACNNNNNGTC 1 cut(s) 430
AjnI CCWGG 8 cut(s) 347, 605, 1114, 1930, 1996, 2376, 2401, 2434
AjuI GAANNNNNNNTTGG 2 cut(s) 2239, 2271
AluBI AGCT 9 cut(s) 134, 166, 767, 1148, 1210, 1291, 1305, 1983, 2288
AluI AGCT 9 cut(s) 134, 166, 767, 1148, 1210, 1291, 1305, 1983, 2288
Alw21I GWGCWC 1 cut(s) 1768
Alw26I GTCTC 8 cut(s) 201, 1146, 1268, 1498, 2213, 2274, 2387, 2490
AlwI GGATC 8 cut(s) 173, 390, 403, 493, 713, 824, 1625, 2156
AlwNI CAGNNNCTG 2 cut(s) 1343, 1505
Ama87I CYCGRG 1 cut(s) 1564
Aor13HI TCCGGA 1 cut(s) 1636
AoxI GGCC 3 cut(s) 1042, 2437, 2531
ApeKI GCWGC 3 cut(s) 356, 1624, 1952
ApoI RAATTY 5 cut(s) 337, 624, 1333, 1649, 1685
Asp700I GAANNNNTTC 2 cut(s) 341, 808
Asp718I GGTACC 1 cut(s) 2495
AspLEI GCGC 1 cut(s) 2356
AspS9I GGNCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
AsuC2I CCSGG 2 cut(s) 1271, 1434
AsuHPI GGTGA 4 cut(s) 16, 205, 747, 1479
AsuII TTCGAA 1 cut(s) 1350
AvaI CYCGRG 1 cut(s) 1564
AvaII GGWCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
AxyI CCTNAGG 2 cut(s) 1413, 2211
BaeGI GKGCMC 1 cut(s) 843
BaeI ACNNNNGTAYC 2 cut(s) 1826, 1859
BalI TGGCCA 2 cut(s) 1044, 2533
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 2495
BanII GRGCYC 2 cut(s) 530, 568
BauI CACGAG 2 cut(s) 188, 1748
BbrPI CACGTG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 1768
BbvI GCAGC 3 cut(s) 343, 1636, 1964
BceAI ACGGC 1 cut(s) 1175
BcgI CGANNNNNNTGC 4 cut(s) 572, 606, 2319, 2353
BciT130I CCWGG 8 cut(s) 349, 607, 1116, 1932, 1998, 2378, 2403, 2436
BciVI GTATCC 3 cut(s) 217, 1854, 2124
BclI TGATCA 2 cut(s) 919, 1459
BcnI CCSGG 2 cut(s) 1271, 1434
BcoDI GTCTC 8 cut(s) 201, 1146, 1268, 1498, 2213, 2274, 2387, 2490
BfaI CTAG 5 cut(s) 21, 378, 747, 1023, 1476
BfmI CTRYAG 1 cut(s) 328
BfuAI ACCTGC 2 cut(s) 1840, 2086
BfuI GTATCC 3 cut(s) 217, 1854, 2124
BglII AGATCT 3 cut(s) 1478, 1541, 2251
BisI GCNGC 3 cut(s) 357, 1625, 1953
BlsI GCNGC 3 cut(s) 358, 1626, 1954
Bme18I GGWCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
BmeRI GACNNNNNGTC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 1564
BmgT120I GGNCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
BmiI GGNNCC 6 cut(s) 13, 397, 856, 1057, 2433, 2497
BmrI ACTGGG 4 cut(s) 18, 469, 951, 1898
BmsI GCATC 8 cut(s) 94, 133, 380, 592, 1546, 1835, 1983, 2226
BmuI ACTGGG 4 cut(s) 18, 469, 951, 1898
BoxI GACNNNNGTC 1 cut(s) 1284
BpiI GAAGAC 1 cut(s) 702
BpmI CTGGAG 3 cut(s) 579, 1137, 2019
Bpu14I TTCGAA 1 cut(s) 1350
BpuEI CTTGAG 4 cut(s) 514, 552, 1128, 1312
BpuMI CCSGG 2 cut(s) 1271, 1434
Bsa29I ATCGAT 1 cut(s) 74
BsaAI YACGTR 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 1738
BsaI GGTCTC 1 cut(s) 2387
BsaJI CCNNGG 4 cut(s) 347, 759, 1114, 2377
BsaWI WCCGGW 1 cut(s) 1636
BsaXI ACNNNNNCTCC 4 cut(s) 2110, 2140, 2448, 2478
Bsc4I CCNNNNNNNGG 6 cut(s) 392, 1051, 1931, 1973, 2003, 2072
Bse21I CCTNAGG 2 cut(s) 1413, 2211
Bse3DI GCAATG 1 cut(s) 1713
Bse8I GATNNNNATC 1 cut(s) 1738
BseAI TCCGGA 1 cut(s) 1636
BseBI CCWGG 8 cut(s) 349, 607, 1116, 1932, 1998, 2378, 2403, 2436
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 4 cut(s) 347, 759, 1114, 2377
BseGI GGATG 7 cut(s) 148, 1561, 1717, 1790, 2241, 2318, 2521
BseJI GATNNNNATC 1 cut(s) 1738
BseLI CCNNNNNNNGG 6 cut(s) 392, 1051, 1931, 1973, 2003, 2072
BseMI GCAATG 1 cut(s) 1713
BseMII CTCAG 9 cut(s) 625, 1404, 1497, 1670, 1953, 2208, 2217, 2225, 2455
BseRI GAGGAG 3 cut(s) 336, 2466, 2469
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 3 cut(s) 343, 1636, 1964
BseYI CCCAGC 1 cut(s) 1948
BsgI GTGCAG 1 cut(s) 921
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 3 cut(s) 1044, 2439, 2533
BshNI GGYRCC 1 cut(s) 2495
BshVI ATCGAT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 1768
BsiHKCI CYCGRG 1 cut(s) 1564
BsiSI CCGG 3 cut(s) 1271, 1433, 1637
BslFI GGGAC 5 cut(s) 437, 1253, 2104, 2166, 2445
BslI CCNNNNNNNGG 6 cut(s) 392, 1051, 1931, 1973, 2003, 2072
BsmAI GTCTC 8 cut(s) 201, 1146, 1268, 1498, 2213, 2274, 2387, 2490
BsmBI CGTCTC 2 cut(s) 1498, 2490
BsmFI GGGAC 5 cut(s) 437, 1253, 2104, 2166, 2445
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 3 cut(s) 1044, 2439, 2533
Bso31I GGTCTC 1 cut(s) 2387
BsoBI CYCGRG 1 cut(s) 1564
Bsp119I TTCGAA 1 cut(s) 1350
Bsp1286I GDGCHC 4 cut(s) 530, 568, 843, 1768
Bsp13I TCCGGA 1 cut(s) 1636
BspACI CCGC 6 cut(s) 24, 1282, 1847, 2004, 2081, 2420
BspANI GGCC 3 cut(s) 1044, 2439, 2533
BspCNI CTCAG 9 cut(s) 624, 1405, 1498, 1669, 1952, 2209, 2218, 2224, 2454
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 1636
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 6 cut(s) 13, 397, 856, 1057, 2433, 2497
BspMI ACCTGC 2 cut(s) 1840, 2086
BspPI GGATC 8 cut(s) 173, 390, 403, 493, 713, 824, 1625, 2156
BspT104I TTCGAA 1 cut(s) 1350
BspT107I GGYRCC 1 cut(s) 2495
BspTNI GGTCTC 1 cut(s) 2387
BsrDI GCAATG 1 cut(s) 1713
BssECI CCNNGG 4 cut(s) 347, 759, 1114, 2377
BssSI CACGAG 2 cut(s) 188, 1748
Bst2BI CACGAG 2 cut(s) 188, 1748
Bst2UI CCWGG 8 cut(s) 349, 607, 1116, 1932, 1998, 2378, 2403, 2436
Bst4CI ACNGT 6 cut(s) 229, 332, 514, 926, 1515, 1720
Bst6I CTCTTC 5 cut(s) 191, 447, 562, 1369, 2390
BstAPI GCANNNNNTGC 1 cut(s) 1958
BstBAI YACGTR 1 cut(s) 941
BstBI TTCGAA 1 cut(s) 1350
BstC8I GCNNGC 2 cut(s) 243, 1824
BstDEI CTNAG 9 cut(s) 611, 1413, 1506, 1656, 1939, 2211, 2217, 2226, 2441
BstDSI CCRYGG 1 cut(s) 759
BstEII GGTNACC 1 cut(s) 1943
BstF5I GGATG 7 cut(s) 148, 1561, 1717, 1790, 2241, 2318, 2521
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 1 cut(s) 2356
BstMAI GTCTC 8 cut(s) 201, 1146, 1268, 1498, 2213, 2274, 2387, 2490
BstMWI GCNNNNNNNGC 4 cut(s) 152, 1207, 1819, 1958
BstNI CCWGG 8 cut(s) 349, 607, 1116, 1932, 1998, 2378, 2403, 2436
BstNSI RCATGY 3 cut(s) 1220, 1774, 2371
BstPAI GACNNNNGTC 1 cut(s) 1284
BstPI GGTNACC 1 cut(s) 1943
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 843
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 343, 1636, 1964
BstV2I GAAGAC 1 cut(s) 702
BstX2I RGATCY 9 cut(s) 178, 395, 718, 829, 1478, 1541, 1630, 2161, 2251
BstXI CCANNNNNNTGG 3 cut(s) 482, 964, 1177
BstYI RGATCY 9 cut(s) 178, 395, 718, 829, 1478, 1541, 1630, 2161, 2251
Bsu15I ATCGAT 1 cut(s) 74
Bsu36I CCTNAGG 2 cut(s) 1413, 2211
BsuI GTATCC 3 cut(s) 217, 1854, 2124
BsuRI GGCC 3 cut(s) 1044, 2439, 2533
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 1 cut(s) 759
BtgZI GCGATG 1 cut(s) 1234
BtsCI GGATG 7 cut(s) 148, 1561, 1717, 1790, 2241, 2318, 2521
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 5 cut(s) 117, 283, 510, 964, 2449
BveI ACCTGC 2 cut(s) 1840, 2086
Cac8I GCNNGC 2 cut(s) 243, 1824
CaiI CAGNNNCTG 2 cut(s) 1343, 1505
CfoI GCGC 1 cut(s) 2356
Cfr13I GGNCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
ClaI ATCGAT 1 cut(s) 74
Csp6I GTAC 7 cut(s) 294, 731, 1561, 1693, 1721, 2406, 2496
CviQI GTAC 7 cut(s) 294, 731, 1561, 1693, 1721, 2406, 2496
DdeI CTNAG 9 cut(s) 611, 1413, 1506, 1656, 1939, 2211, 2217, 2226, 2441
DraIII CACNNNGTG 1 cut(s) 193
DrdI GACNNNNNNGTC 1 cut(s) 1490
DriI GACNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 1490
EaeI YGGCCR 2 cut(s) 1042, 2531
Eam1104I CTCTTC 5 cut(s) 191, 447, 562, 1369, 2390
Eam1105I GACNNNNNGTC 1 cut(s) 430
EarI CTCTTC 5 cut(s) 191, 447, 562, 1369, 2390
Eco24I GRGCYC 2 cut(s) 530, 568
Eco31I GGTCTC 1 cut(s) 2387
Eco47I GGWCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
Eco57I CTGAAG 3 cut(s) 1350, 1404, 2249
Eco72I CACGTG 1 cut(s) 941
Eco81I CCTNAGG 2 cut(s) 1413, 2211
Eco88I CYCGRG 1 cut(s) 1564
Eco91I GGTNACC 1 cut(s) 1943
EcoO109I RGGNCCY 2 cut(s) 855, 2432
EcoO65I GGTNACC 1 cut(s) 1943
EcoRI GAATTC 1 cut(s) 1333
EcoRII CCWGG 8 cut(s) 347, 605, 1114, 1930, 1996, 2376, 2401, 2434
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 2 cut(s) 530, 568
Esp3I CGTCTC 2 cut(s) 1498, 2490
FaqI GGGAC 5 cut(s) 437, 1253, 2104, 2166, 2445
FauI CCCGC 1 cut(s) 2074
FauNDI CATATG 1 cut(s) 1957
FbaI TGATCA 2 cut(s) 919, 1459
Fnu4HI GCNGC 3 cut(s) 357, 1625, 1953
FokI GGATG 7 cut(s) 155, 1568, 1724, 1777, 2248, 2305, 2508
FriOI GRGCYC 2 cut(s) 530, 568
Fsp4HI GCNGC 3 cut(s) 357, 1625, 1953
FspBI CTAG 5 cut(s) 21, 378, 747, 1023, 1476
FspI TGCGCA 1 cut(s) 2355
GlaI GCGC 1 cut(s) 2355
GluI GCNGC 3 cut(s) 357, 1625, 1953
GsaI CCCAGC 1 cut(s) 1952
GsuI CTGGAG 3 cut(s) 579, 1137, 2019
HaeIII GGCC 3 cut(s) 1044, 2439, 2533
HapII CCGG 3 cut(s) 1271, 1433, 1637
HhaI GCGC 1 cut(s) 2356
Hin6I GCGC 1 cut(s) 2354
HinP1I GCGC 1 cut(s) 2354
HindIII AAGCTT 1 cut(s) 1208
HpaII CCGG 3 cut(s) 1271, 1433, 1637
HphI GGTGA 4 cut(s) 16, 205, 747, 1479
Hpy166II GTNNAC 4 cut(s) 296, 517, 1595, 2075
Hpy8I GTNNAC 4 cut(s) 296, 517, 1595, 2075
Hpy99I CGWCG 1 cut(s) 1155
HpyAV CCTTC 4 cut(s) 744, 819, 1977, 2273
HpyCH4III ACNGT 6 cut(s) 229, 332, 514, 926, 1515, 1720
HpyCH4IV ACGT 4 cut(s) 775, 940, 2199, 2283
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 1207, 1819, 1958
HpyF3I CTNAG 9 cut(s) 611, 1413, 1506, 1656, 1939, 2211, 2217, 2226, 2441
HpySE526I ACGT 4 cut(s) 775, 940, 2199, 2283
HspAI GCGC 1 cut(s) 2354
Kpn2I TCCGGA 1 cut(s) 1636
KpnI GGTACC 1 cut(s) 2499
Ksp22I TGATCA 2 cut(s) 919, 1459
LmnI GCTCC 3 cut(s) 598, 764, 1055
Lsp1109I GCAGC 3 cut(s) 343, 1636, 1964
LweI GCATC 8 cut(s) 94, 133, 380, 592, 1546, 1835, 1983, 2226
MaeI CTAG 5 cut(s) 21, 378, 747, 1023, 1476
MaeII ACGT 4 cut(s) 775, 940, 2199, 2283
MflI RGATCY 9 cut(s) 178, 395, 718, 829, 1478, 1541, 1630, 2161, 2251
MhlI GDGCHC 4 cut(s) 530, 568, 843, 1768
MlsI TGGCCA 2 cut(s) 1044, 2533
MluNI TGGCCA 2 cut(s) 1044, 2533
MlyI GAGTC 4 cut(s) 36, 953, 1478, 2466
MmeI TCCRAC 6 cut(s) 423, 1084, 2090, 2119, 2141, 2434
Mox20I TGGCCA 2 cut(s) 1044, 2533
Mph1103I ATGCAT 1 cut(s) 373
MroI TCCGGA 1 cut(s) 1636
MroXI GAANNNNTTC 2 cut(s) 341, 808
MscI TGGCCA 2 cut(s) 1044, 2533
MseI TTAA 7 cut(s) 78, 125, 495, 771, 1320, 1760, 1775
MslI CAYNNNNRTG 4 cut(s) 102, 1175, 1227, 1263
Msp20I TGGCCA 2 cut(s) 1044, 2533
MspA1I CMGCKG 1 cut(s) 1284
MspI CCGG 3 cut(s) 1271, 1433, 1637
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 8 cut(s) 349, 607, 1116, 1932, 1998, 2378, 2403, 2436
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 152, 1207, 1819, 1958
NciI CCSGG 2 cut(s) 1271, 1434
NdeI CATATG 1 cut(s) 1957
NlaIV GGNNCC 6 cut(s) 13, 397, 856, 1057, 2433, 2497
NmeAIII GCCGAG 1 cut(s) 1475
NmuCI GTSAC 3 cut(s) 4, 95, 2394
NsbI TGCGCA 1 cut(s) 2355
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 3 cut(s) 1220, 1774, 2371
NspV TTCGAA 1 cut(s) 1350
PciI ACATGT 2 cut(s) 1216, 1770
PctI GAATGC 1 cut(s) 463
PdmI GAANNNNTTC 2 cut(s) 341, 808
PfeI GAWTC 8 cut(s) 67, 169, 572, 1340, 1355, 1382, 2040, 2416
PflFI GACNNNGTC 1 cut(s) 10
PflMI CCANNNNNTGG 1 cut(s) 2072
PfoI TCCNGGA 1 cut(s) 1269
PkrI GCNGC 3 cut(s) 358, 1626, 1954
PleI GAGTC 4 cut(s) 36, 952, 1478, 2465
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
PpsI GAGTC 4 cut(s) 36, 952, 1478, 2465
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 2 cut(s) 855, 2432
PscI ACATGT 2 cut(s) 1216, 1770
PshAI GACNNNNGTC 1 cut(s) 1284
Psp5II RGGWCCY 2 cut(s) 855, 2432
Psp6I CCWGG 8 cut(s) 347, 605, 1114, 1930, 1996, 2376, 2401, 2434
PspCI CACGTG 1 cut(s) 941
PspEI GGTNACC 1 cut(s) 1943
PspFI CCCAGC 1 cut(s) 1948
PspGI CCWGG 8 cut(s) 347, 605, 1114, 1930, 1996, 2376, 2401, 2434
PspN4I GGNNCC 6 cut(s) 13, 397, 856, 1057, 2433, 2497
PspPI GGNCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
PspPPI RGGWCCY 2 cut(s) 855, 2432
PstNI CAGNNNCTG 2 cut(s) 1343, 1505
PsuI RGATCY 9 cut(s) 178, 395, 718, 829, 1478, 1541, 1630, 2161, 2251
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 7 cut(s) 295, 732, 1562, 1694, 1722, 2407, 2497
RsaNI GTAC 7 cut(s) 294, 731, 1561, 1693, 1721, 2406, 2496
RseI CAYNNNNRTG 4 cut(s) 102, 1175, 1227, 1263
SaqAI TTAA 7 cut(s) 78, 125, 495, 771, 1320, 1760, 1775
SatI GCNGC 3 cut(s) 357, 1625, 1953
Sau96I GGNCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
SchI GAGTC 4 cut(s) 36, 953, 1478, 2466
SduI GDGCHC 4 cut(s) 530, 568, 843, 1768
SfaNI GCATC 8 cut(s) 94, 133, 380, 592, 1546, 1835, 1983, 2226
SfcI CTRYAG 1 cut(s) 328
SfuI TTCGAA 1 cut(s) 1350
SinI GGWCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
SmiMI CAYNNNNRTG 4 cut(s) 102, 1175, 1227, 1263
SmlI CTYRAG 4 cut(s) 529, 567, 1143, 1327
SmoI CTYRAG 4 cut(s) 529, 567, 1143, 1327
SsiI CCGC 6 cut(s) 24, 1282, 1847, 2004, 2081, 2420
SspI AATATT 2 cut(s) 493, 2194
SspMI CTAG 5 cut(s) 21, 378, 747, 1023, 1476
TaaI ACNGT 6 cut(s) 229, 332, 514, 926, 1515, 1720
TaiI ACGT 4 cut(s) 778, 943, 2202, 2286
TaqI TCGA 5 cut(s) 45, 74, 892, 928, 1350
TfiI GAWTC 8 cut(s) 67, 169, 572, 1340, 1355, 1382, 2040, 2416
Tru1I TTAA 7 cut(s) 78, 125, 495, 771, 1320, 1760, 1775
Tru9I TTAA 7 cut(s) 78, 125, 495, 771, 1320, 1760, 1775
TscAI CASTG 5 cut(s) 124, 283, 517, 964, 2449
TseFI GTSAC 3 cut(s) 4, 95, 2394
TseI GCWGC 3 cut(s) 356, 1624, 1952
Tsp45I GTSAC 3 cut(s) 4, 95, 2394
TspDTI ATGAA 7 cut(s) 66, 161, 465, 716, 1211, 1698, 1728
TspGWI ACGGA 2 cut(s) 1680, 2472
TspRI CASTG 5 cut(s) 124, 283, 517, 964, 2449
Tth111I GACNNNGTC 1 cut(s) 10
Van91I CCANNNNNTGG 1 cut(s) 2072
VpaK11BI GGWCC 8 cut(s) 12, 855, 875, 1430, 1592, 1852, 2075, 2432
XapI RAATTY 5 cut(s) 337, 624, 1333, 1649, 1685
XbaI TCTAGA 2 cut(s) 746, 1475
XceI RCATGY 3 cut(s) 1220, 1774, 2371
XcmI CCANNNNNNNNNTGG 1 cut(s) 2432
XmnI GAANNNNTTC 2 cut(s) 341, 808
XspI CTAG 5 cut(s) 21, 378, 747, 1023, 1476
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.