Rw2G036110

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005772
Physical Location & Seq
Forward (+)
59219007 .. 59227749
8743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036110.1

Sequence Viewer

Length: 2670 bp
ATGGTCTCTGGGTCGCGAATCGAAGGTGGTGGTGGGACTCAGATAATCTCCGCCGGAGTGAGGAAAACCATCCAATCCATCAAAGAAATAGTGGGCAATCACTCTGATATTGATATCTATTGGGCTCTCAAAGAGACCGATATGGATCCTAATGAAACTGCCCAGAAATTACTCAACCAAGACCCATTTCATGAGGTGAAGAGAAAAAGAGACAAAAAGAAGGACGTGTCACCTAAGAGTATCGGGCAAAAGGTTCCTACCGAGCCAAGAAGACATTTTGAGAGTGCTGGTCAAGGACCAAGATCAAATACAATTTCAGATTGTATTGTGGAGCCTAAAAGACATTTTGAGAGTGCAGGTCAAGGACCTAGATCAAATACAATTTCTGATCGTAATGTCGAGCCTGGAAGACATTTTGACAGTGCAGGTCAAGGACCAAAATCTAATTCATTTTCTGATCGAAATGTTGAGCCAAGAAGACATTTTGAGAGTGCAGGTCAAGGACCAAGACATAATTCATTTTCTGATCGTAATGTTAGAAGAGGAGGTTATGTGCGCAGGGGTTTACCTGGAATCAGCAGAGAGTTTCGTGTTGTGAGAGACAACAGAGCTAACCAAAATATGGATGGAGAAATTAAGCCTGCTTCACCTCAATGTACTACATCCACGAACGAGCAAGTGATTTCAAATGTTTCTGAGAAGGGCCAAACAGGAAATTCAAGCAGTCAAAAGCCATCTAATAGACAGCATTCCTCACAAGCATTGAATGGGCAAACTGATTCTCGAATTAGAACGAACGTTGCTAATTCAACTGGTATGATTAGAAAGGAGACATTAGTGGAGAAGCGAGTTGCTCTTCCAAATTCAGTTTCACGGGTACAGGCAGGGAAACCAAACAACTCCCAACCACATTCTGCATCAAGCACTTCTGTTATTGGGGTGTATTCCTCGTCCACAGATCCTGTTCATGTGCCGTCTCCTGATTCTAGACCATCTGCTTCTGTTGGTGCAATTAAGCGCGAAGTTGGGGTGGTTGGAGTTCGGAAACAGTCTTCTGACAACTCCAAGTCAGCTGTACCAAGTAGTTCTTTCTCTAATTCACTTTTGGGAAAAGAGGGCTCTGCAGAATCATTTAGATCTTTCACTGGTATCTCTAAACCTGATCAACTCAGCCAAACTTCTGAATCTGTGATGCCGGTCAGCAGATCTTTCGTAAGTAATCAGCACAGTGTCAGGGCGCATCAACAACCTGTGGGTCATCAGAAAGCTTCCCAGCCTAACAAGGAGTGGAAACCTAAATCAAGTCAGAAGCCAAGCCCTAATAGTCCTGGAGTCATTGGAACACCAACAAAATCTGCTTCTCCTCTTTCTGATGATTCAAAGGTCTCAGAGTCAGAAGCGGCTAAGTTGCAAGATAAGCTTGCCCGAGTAAATATATATGAGAACTGTAATGTTGTGATAGCGCAAAATATTAGAGTCCCTGAGAGTGACCAGTTTGATTCAACAGGAAACACAGTTAACGGATTTCAAGCTGGAGCTGCGGAGGAGTTGAACAAGGAACCTCCAGCAAGTTTGTCAACATCAGCTCCTGAGTCGCGCAGTGATGAGGCTTCCGGCACGAAGCCAGTAGATTTATTAGATGACCAAGTTAGAAATTCTGGATCTGATTTTTCGGCACCGTCTGCAGTGCCTGAGCATTTGCCTGAGAAAAAAGAGACCTCAAGTCCTCAGAGTTTGGACAATTATGCAGATATTGGTTTGGTTCGAGACAACAGTCCGTCCTTTGCACCTTCAGATTCGCAGAACCAAGATCCACCAGAGTTACAAGGTTTTACGGCATTTGATCCTCAGACTGGTTATGATATACCTTATTACAGACCCAGTATGGATGAAAGTGTTTGCGGACAGGGTCTACCATCTCCTCAGGAGTTCAATAATGCCAACAGTATTCCTGCATCAACAGTTGCCATGGTACAACAGCAACCACCGCATGTGGCGCAGATGTACCCACAAGTTCATGTTTCACATTATGCTAATCTTATGCCATATCGTCAGTTTATCTCACCAGTATACGTTCCACCAATGGCTGTGCCTGGCTATTCCAATAATCCTGCCTATCCTCACATGTCTAATGGCAACAGCTACTTGCTGATGCCTGGAGGTGGTTCCCACCTAAATGCAAACAGCCTCAAGTATGGAGTTCAGCAATTTAAGCCCGTCCCTGCTGGAAGTCCCACTGGGTTTGGAAATTTTACTAACCCAGCAGGTTATGCAATGAATGCCCCTGGTGTAGTTGGAGGTGCCACTGGACTCGAAGATTCATCAAGAATGAAGTACAAAGATGGCAATCTTTATGTTCCAAATCCACAGGCGGAGACATCAGAGATTTGGATTCAGAACCCAAGGGAGCATCCAGGAATGCAATCCACACCATACTACAACATGCCTGGCAAACACCTCATGCTCCTTATATGCCCTGCAGCACAATCTTCGCACATGCAATTCCCGGGAATGTACCATCCTCCTCAGCCTGCTGCAATGGCCAGTCCGCACCATATGGGCCCTGCTATGCCCGGCAATGTCGGAGTGGGGGTGGCCGCAGCTGCTCCTGGGGCACAGGCCTATCAGCAGCCCCAGTTGAATCATATGAACTGGACAACAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

889

Amino Acids

95.86

Weight (kDa)

8.73

Isoelectric Point (pI)

56.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 16 - 74 4.1e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 557
Acc36I ACCTGC 4 cut(s) 347, 416, 485, 2255
AccB1I GGYRCC 2 cut(s) 1675, 2300
AccB7I CCANNNNNTGG 1 cut(s) 622
AccI GTMKAC 2 cut(s) 1912, 2070
AccII CGCG 3 cut(s) 16, 1020, 1597
AciI CCGC 8 cut(s) 51, 1400, 1541, 1902, 1988, 2372, 2549, 2598
AclI AACGTT 1 cut(s) 798
AclWI GGATC 6 cut(s) 140, 153, 953, 1669, 1805, 1838
AcoI YGGCCR 2 cut(s) 2541, 2595
AcsI RAATTY 4 cut(s) 715, 862, 1654, 2248
AcuI CTGAAG 1 cut(s) 1776
AfaI GTAC 7 cut(s) 658, 879, 1077, 1974, 2006, 2336, 2516
AfiI CCNNNNNNNGG 4 cut(s) 60, 622, 1853, 2162
AflIII ACRYGT 2 cut(s) 225, 2124
AhdI GACNNNNNGTC 1 cut(s) 1722
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 9 cut(s) 403, 568, 1327, 2092, 2155, 2284, 2413, 2446, 2608
AjuI GAANNNNNNNTTGG 8 cut(s) 171, 203, 430, 462, 499, 531, 853, 885
AluBI AGCT 9 cut(s) 611, 1073, 1268, 1420, 1532, 1538, 1586, 2142, 2603
AluI AGCT 9 cut(s) 611, 1073, 1268, 1420, 1532, 1538, 1586, 2142, 2603
AlwI GGATC 6 cut(s) 140, 153, 953, 1669, 1805, 1838
AlwNI CAGNNNCTG 3 cut(s) 962, 1589, 1691
Ama87I CYCGRG 2 cut(s) 1425, 2506
AoxI GGCC 5 cut(s) 703, 2541, 2560, 2595, 2619
ApaI GGGCCC 1 cut(s) 2564
ApeKI GCWGC 6 cut(s) 1538, 2480, 2534, 2600, 2603, 2629
ApoI RAATTY 4 cut(s) 715, 862, 1654, 2248
AspLEI GCGC 6 cut(s) 558, 1020, 1240, 1465, 1599, 1999
AspS9I GGNCC 7 cut(s) 296, 365, 434, 503, 703, 2560, 2561
AsuC2I CCSGG 3 cut(s) 2507, 2508, 2574
AsuHPI GGTGA 4 cut(s) 208, 222, 639, 2055
AvaI CYCGRG 2 cut(s) 1425, 2506
AvaII GGWCC 4 cut(s) 296, 365, 434, 503
AxyI CCTNAGG 1 cut(s) 1923
BaeGI GKGCMC 2 cut(s) 2564, 2617
BalI TGGCCA 1 cut(s) 2543
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 2 cut(s) 1675, 2300
BanII GRGCYC 3 cut(s) 127, 1121, 2564
BbsI GAAGAC 4 cut(s) 277, 415, 484, 1044
BbvCI CCTCAGC 1 cut(s) 2526
BbvI GCAGC 6 cut(s) 1525, 2492, 2521, 2590, 2612, 2641
BccI CCATC 8 cut(s) 77, 86, 620, 742, 1000, 1924, 2336, 2526
BceAI ACGGC 2 cut(s) 958, 1851
BcgI CGANNNNNNTGC 4 cut(s) 1192, 1226, 2472, 2506
BciT130I CCWGG 9 cut(s) 405, 570, 1329, 2094, 2157, 2286, 2415, 2448, 2610
BclI TGATCA 1 cut(s) 1162
BcnI CCSGG 3 cut(s) 2507, 2508, 2574
BfaI CTAG 2 cut(s) 369, 987
BfmI CTRYAG 3 cut(s) 1122, 1683, 2478
BfuAI ACCTGC 4 cut(s) 347, 416, 485, 2255
BglII AGATCT 2 cut(s) 1136, 1205
BisI GCNGC 8 cut(s) 1401, 1539, 2481, 2535, 2598, 2601, 2604, 2630
BlsI GCNGC 8 cut(s) 1402, 1540, 2482, 2536, 2599, 2602, 2605, 2631
Bme18I GGWCC 4 cut(s) 296, 365, 434, 503
BmeRI GACNNNNNGTC 1 cut(s) 1722
BmeT110I CYCGRG 2 cut(s) 1425, 2506
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 7 cut(s) 296, 365, 434, 503, 703, 2560, 2561
BmiI GGNNCC 8 cut(s) 147, 255, 333, 1560, 1677, 2167, 2302, 2562
BmrI ACTGGG 3 cut(s) 1875, 2247, 2629
BmsI GCATC 6 cut(s) 926, 1182, 1249, 1964, 2142, 2419
BmuI ACTGGG 3 cut(s) 1875, 2247, 2629
BoxI GACNNNNGTC 1 cut(s) 1773
BpiI GAAGAC 4 cut(s) 277, 415, 484, 1044
BpmI CTGGAG 4 cut(s) 1350, 1548, 1554, 2178
Bpu10I CCTNAGC 2 cut(s) 1692, 2526
BpuEI CTTGAG 2 cut(s) 1705, 2174
BpuMI CCSGG 3 cut(s) 2507, 2508, 2574
BsaBI GATNNNNATC 2 cut(s) 144, 2346
BsaI GGTCTC 4 cut(s) 10, 128, 1390, 1709
BsaJI CCNNGG 5 cut(s) 1968, 2284, 2402, 2506, 2609
BsaXI ACNNNNNCTCC 6 cut(s) 537, 567, 1570, 1600, 2577, 2607
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 622, 1853, 2162
Bse118I RCCGGY 1 cut(s) 1195
Bse21I CCTNAGG 1 cut(s) 1923
Bse3DI GCAATG 3 cut(s) 2280, 2544, 2584
Bse8I GATNNNNATC 2 cut(s) 144, 2346
BseBI CCWGG 9 cut(s) 405, 570, 1329, 2094, 2157, 2286, 2415, 2448, 2610
BseDI CCNNGG 5 cut(s) 1968, 2284, 2402, 2506, 2609
BseGI GGATG 6 cut(s) 69, 631, 662, 1894, 2410, 2518
BseJI GATNNNNATC 2 cut(s) 144, 2346
BseLI CCNNNNNNNGG 4 cut(s) 60, 622, 1853, 2162
BseMI GCAATG 3 cut(s) 2280, 2544, 2584
BseRI GAGGAG 5 cut(s) 558, 1353, 1559, 1911, 2514
BseSI GKGCMC 2 cut(s) 2564, 2617
BseXI GCAGC 6 cut(s) 1525, 2492, 2521, 2590, 2612, 2641
BseYI CCCAGC 2 cut(s) 1272, 2260
BsgI GTGCAG 3 cut(s) 375, 444, 513
Bsh1236I CGCG 3 cut(s) 16, 1020, 1597
BshFI GGCC 5 cut(s) 705, 2543, 2562, 2597, 2621
BshNI GGYRCC 2 cut(s) 1675, 2300
BsiHKCI CYCGRG 2 cut(s) 1425, 2506
BsiSI CCGG 5 cut(s) 54, 1196, 1614, 2507, 2574
BslFI GGGAC 4 cut(s) 49, 1463, 2204, 2217
BslI CCNNNNNNNGG 4 cut(s) 60, 622, 1853, 2162
BsmBI CGTCTC 1 cut(s) 981
BsmFI GGGAC 4 cut(s) 49, 1463, 2204, 2217
BsmI GAATGC 3 cut(s) 748, 2284, 2424
BsnI GGCC 5 cut(s) 705, 2543, 2562, 2597, 2621
Bso31I GGTCTC 4 cut(s) 10, 128, 1390, 1709
BsoBI CYCGRG 2 cut(s) 1425, 2506
Bsp120I GGGCCC 1 cut(s) 2560
Bsp1286I GDGCHC 4 cut(s) 127, 1121, 2564, 2617
Bsp19I CCATGG 1 cut(s) 1968
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 8 cut(s) 51, 1400, 1541, 1902, 1988, 2372, 2549, 2598
BspANI GGCC 5 cut(s) 705, 2543, 2562, 2597, 2621
BspFNI CGCG 3 cut(s) 16, 1020, 1597
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 8 cut(s) 147, 255, 333, 1560, 1677, 2167, 2302, 2562
BspMAI CTGCAG 3 cut(s) 1126, 1687, 2482
BspMI ACCTGC 4 cut(s) 347, 416, 485, 2255
BspPI GGATC 6 cut(s) 140, 153, 953, 1669, 1805, 1838
BspQI GCTCTTC 1 cut(s) 861
BspT107I GGYRCC 2 cut(s) 1675, 2300
BspTNI GGTCTC 4 cut(s) 10, 128, 1390, 1709
BsrDI GCAATG 3 cut(s) 2280, 2544, 2584
BsrFI RCCGGY 1 cut(s) 1195
BssAI RCCGGY 1 cut(s) 1195
BssECI CCNNGG 5 cut(s) 1968, 2284, 2402, 2506, 2609
BssNAI GTATAC 1 cut(s) 2071
BssT1I CCWWGG 2 cut(s) 1968, 2402
Bst1107I GTATAC 1 cut(s) 2071
Bst2UI CCWGG 9 cut(s) 405, 570, 1329, 2094, 2157, 2286, 2415, 2448, 2610
Bst4CI ACNGT 9 cut(s) 422, 1050, 1229, 1448, 1516, 1680, 1775, 1946, 1963
Bst6I CTCTTC 3 cut(s) 194, 535, 861
BstAPI GCANNNNNTGC 3 cut(s) 1682, 2270, 2279
BstC8I GCNNGC 3 cut(s) 642, 1422, 2532
BstDSI CCRYGG 1 cut(s) 1968
BstF5I GGATG 6 cut(s) 69, 631, 662, 1894, 2410, 2518
BstFNI CGCG 3 cut(s) 16, 1020, 1597
BstHHI GCGC 6 cut(s) 558, 1020, 1240, 1465, 1599, 1999
BstNI CCWGG 9 cut(s) 405, 570, 1329, 2094, 2157, 2286, 2415, 2448, 2610
BstNSI RCATGY 4 cut(s) 1994, 2128, 2446, 2500
BstPAI GACNNNNGTC 1 cut(s) 1773
BstSFI CTRYAG 3 cut(s) 1122, 1683, 2478
BstSLI GKGCMC 2 cut(s) 2564, 2617
BstUI CGCG 3 cut(s) 16, 1020, 1597
BstV1I GCAGC 6 cut(s) 1525, 2492, 2521, 2590, 2612, 2641
BstV2I GAAGAC 4 cut(s) 277, 415, 484, 1044
BstX2I RGATCY 6 cut(s) 145, 958, 1136, 1205, 1661, 1810
BstYI RGATCY 6 cut(s) 145, 958, 1136, 1205, 1661, 1810
BstZ17I GTATAC 1 cut(s) 2071
Bsu36I CCTNAGG 1 cut(s) 1923
BsuRI GGCC 5 cut(s) 705, 2543, 2562, 2597, 2621
BtgI CCRYGG 1 cut(s) 1968
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 6 cut(s) 69, 631, 662, 1894, 2410, 2518
BtsI GCAGTG 2 cut(s) 1606, 1692
BtsIMutI CAGTG 7 cut(s) 427, 1143, 1234, 1606, 1692, 2235, 2304
BtuMI TCGCGA 1 cut(s) 16
BveI ACCTGC 4 cut(s) 347, 416, 485, 2255
Cac8I GCNNGC 3 cut(s) 642, 1422, 2532
CaiI CAGNNNCTG 3 cut(s) 962, 1589, 1691
CciI TCATGA 1 cut(s) 190
CfoI GCGC 6 cut(s) 558, 1020, 1240, 1465, 1599, 1999
Cfr10I RCCGGY 1 cut(s) 1195
Cfr13I GGNCC 7 cut(s) 296, 365, 434, 503, 703, 2560, 2561
Cfr9I CCCGGG 1 cut(s) 2506
Csp6I GTAC 7 cut(s) 657, 878, 1076, 1973, 2005, 2335, 2515
CspCI CAANNNNNGTGG 2 cut(s) 2224, 2259
CviAII CATG 9 cut(s) 191, 968, 1969, 1991, 2018, 2125, 2443, 2461, 2497
CviQI GTAC 7 cut(s) 657, 878, 1076, 1973, 2005, 2335, 2515
DriI GACNNNNNGTC 1 cut(s) 1722
EaeI YGGCCR 2 cut(s) 2541, 2595
Eam1104I CTCTTC 3 cut(s) 194, 535, 861
Eam1105I GACNNNNNGTC 1 cut(s) 1722
EarI CTCTTC 3 cut(s) 194, 535, 861
EciI GGCGGA 2 cut(s) 40, 2387
Eco130I CCWWGG 2 cut(s) 1968, 2402
Eco147I AGGCCT 1 cut(s) 2621
Eco24I GRGCYC 3 cut(s) 127, 1121, 2564
Eco31I GGTCTC 4 cut(s) 10, 128, 1390, 1709
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 4 cut(s) 296, 365, 434, 503
Eco57I CTGAAG 1 cut(s) 1776
Eco81I CCTNAGG 1 cut(s) 1923
Eco88I CYCGRG 2 cut(s) 1425, 2506
EcoO109I RGGNCCY 2 cut(s) 365, 2561
EcoRII CCWGG 9 cut(s) 403, 568, 1327, 2092, 2155, 2284, 2413, 2446, 2608
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 1968, 2402
EcoT38I GRGCYC 3 cut(s) 127, 1121, 2564
ErhI CCWWGG 2 cut(s) 1968, 2402
Esp3I CGTCTC 1 cut(s) 981
FaeI CATG 9 cut(s) 194, 971, 1972, 1994, 2021, 2128, 2446, 2464, 2500
FalI AAGNNNNNCTT 4 cut(s) 1072, 1104, 1404, 1436
FaqI GGGAC 4 cut(s) 49, 1463, 2204, 2217
FatI CATG 9 cut(s) 190, 967, 1968, 1990, 2017, 2124, 2442, 2460, 2496
FauNDI CATATG 2 cut(s) 2556, 2646
FbaI TGATCA 1 cut(s) 1162
FblI GTMKAC 2 cut(s) 1912, 2070
Fnu4HI GCNGC 8 cut(s) 1401, 1539, 2481, 2535, 2598, 2601, 2604, 2630
FokI GGATG 6 cut(s) 56, 638, 649, 1901, 2397, 2505
FriOI GRGCYC 3 cut(s) 127, 1121, 2564
Fsp4HI GCNGC 8 cut(s) 1401, 1539, 2481, 2535, 2598, 2601, 2604, 2630
FspBI CTAG 2 cut(s) 369, 987
FspI TGCGCA 1 cut(s) 557
GlaI GCGC 6 cut(s) 557, 1019, 1239, 1464, 1598, 1998
GluI GCNGC 8 cut(s) 1401, 1539, 2481, 2535, 2598, 2601, 2604, 2630
GsaI CCCAGC 2 cut(s) 1276, 2264
GsuI CTGGAG 4 cut(s) 1350, 1548, 1554, 2178
HaeIII GGCC 5 cut(s) 705, 2543, 2562, 2597, 2621
HapII CCGG 5 cut(s) 54, 1196, 1614, 2507, 2574
HhaI GCGC 6 cut(s) 558, 1020, 1240, 1465, 1599, 1999
Hin1II CATG 9 cut(s) 194, 971, 1972, 1994, 2021, 2128, 2446, 2464, 2500
Hin6I GCGC 6 cut(s) 556, 1018, 1238, 1463, 1597, 1997
HinP1I GCGC 6 cut(s) 556, 1018, 1238, 1463, 1597, 1997
HincII GTYRAC 2 cut(s) 1519, 1578
HindII GTYRAC 2 cut(s) 1519, 1578
HindIII AAGCTT 2 cut(s) 1266, 1418
HpaI GTTAAC 1 cut(s) 1519
HpaII CCGG 5 cut(s) 54, 1196, 1614, 2507, 2574
HphI GGTGA 4 cut(s) 208, 222, 639, 2055
Hpy166II GTNNAC 6 cut(s) 566, 954, 1519, 1578, 1913, 2071
Hpy8I GTNNAC 6 cut(s) 566, 954, 1519, 1578, 1913, 2071
HpyAV CCTTC 4 cut(s) 17, 214, 694, 1800
HpyCH4III ACNGT 9 cut(s) 422, 1050, 1229, 1448, 1516, 1680, 1775, 1946, 1963
HpyCH4IV ACGT 3 cut(s) 225, 798, 2073
HpySE526I ACGT 3 cut(s) 225, 798, 2073
Hsp92II CATG 9 cut(s) 194, 971, 1972, 1994, 2021, 2128, 2446, 2464, 2500
HspAI GCGC 6 cut(s) 556, 1018, 1238, 1463, 1597, 1997
Ksp22I TGATCA 1 cut(s) 1162
KspAI GTTAAC 1 cut(s) 1519
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 6 cut(s) 331, 1535, 1591, 2407, 2469, 2611
Lsp1109I GCAGC 6 cut(s) 1525, 2492, 2521, 2590, 2612, 2641
LweI GCATC 6 cut(s) 926, 1182, 1249, 1964, 2142, 2419
MaeI CTAG 2 cut(s) 369, 987
MaeII ACGT 3 cut(s) 225, 798, 2073
MaeIII GTNAC 3 cut(s) 228, 1487, 1821
MboII GAAGA 9 cut(s) 211, 282, 420, 489, 552, 848, 1044, 2327, 2481
MflI RGATCY 6 cut(s) 145, 958, 1136, 1205, 1661, 1810
MhlI GDGCHC 4 cut(s) 127, 1121, 2564, 2617
MlsI TGGCCA 1 cut(s) 2543
MluNI TGGCCA 1 cut(s) 2543
MlyI GAGTC 6 cut(s) 31, 1341, 1400, 1485, 1601, 2304
MmeI TCCRAC 3 cut(s) 1015, 2275, 2563
Mox20I TGGCCA 1 cut(s) 2543
MscI TGGCCA 1 cut(s) 2543
MseI TTAA 4 cut(s) 636, 1014, 1518, 2211
MslI CAYNNNNRTG 2 cut(s) 652, 2175
Msp20I TGGCCA 1 cut(s) 2543
MspA1I CMGCKG 2 cut(s) 1073, 2603
MspI CCGG 5 cut(s) 54, 1196, 1614, 2507, 2574
Mva1269I GAATGC 3 cut(s) 748, 2284, 2424
MvaI CCWGG 9 cut(s) 405, 570, 1329, 2094, 2157, 2286, 2415, 2448, 2610
MvnI CGCG 3 cut(s) 16, 1020, 1597
NciI CCSGG 3 cut(s) 2507, 2508, 2574
NcoI CCATGG 1 cut(s) 1968
NdeI CATATG 2 cut(s) 2556, 2646
NlaIII CATG 9 cut(s) 194, 971, 1972, 1994, 2021, 2128, 2446, 2464, 2500
NlaIV GGNNCC 8 cut(s) 147, 255, 333, 1560, 1677, 2167, 2302, 2562
NmuCI GTSAC 2 cut(s) 228, 1487
NruI TCGCGA 1 cut(s) 16
NsbI TGCGCA 1 cut(s) 557
NspI RCATGY 4 cut(s) 1994, 2128, 2446, 2500
PagI TCATGA 1 cut(s) 190
PceI AGGCCT 1 cut(s) 2621
PciI ACATGT 1 cut(s) 2124
PciSI GCTCTTC 1 cut(s) 861
PctI GAATGC 3 cut(s) 748, 2284, 2424
PflFI GACNNNGTC 1 cut(s) 1908
PflMI CCANNNNNTGG 1 cut(s) 622
PfoI TCCNGGA 2 cut(s) 1327, 2413
PkrI GCNGC 8 cut(s) 1402, 1540, 2482, 2536, 2599, 2602, 2605, 2631
PleI GAGTC 6 cut(s) 31, 1340, 1399, 1484, 1600, 2304
PpsI GAGTC 6 cut(s) 31, 1340, 1399, 1484, 1600, 2304
PpuMI RGGWCCY 1 cut(s) 365
PscI ACATGT 1 cut(s) 2124
PshAI GACNNNNGTC 1 cut(s) 1773
Psp1406I AACGTT 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 9 cut(s) 403, 568, 1327, 2092, 2155, 2284, 2413, 2446, 2608
PspFI CCCAGC 2 cut(s) 1272, 2260
PspGI CCWGG 9 cut(s) 403, 568, 1327, 2092, 2155, 2284, 2413, 2446, 2608
PspN4I GGNNCC 8 cut(s) 147, 255, 333, 1560, 1677, 2167, 2302, 2562
PspOMI GGGCCC 1 cut(s) 2560
PspPI GGNCC 7 cut(s) 296, 365, 434, 503, 703, 2560, 2561
PspPPI RGGWCCY 1 cut(s) 365
PstI CTGCAG 3 cut(s) 1126, 1687, 2482
PstNI CAGNNNCTG 3 cut(s) 962, 1589, 1691
PsuI RGATCY 6 cut(s) 145, 958, 1136, 1205, 1661, 1810
PsyI GACNNNGTC 1 cut(s) 1908
PvuII CAGCTG 2 cut(s) 1073, 2603
RruI TCGCGA 1 cut(s) 16
RsaI GTAC 7 cut(s) 658, 879, 1077, 1974, 2006, 2336, 2516
RsaNI GTAC 7 cut(s) 657, 878, 1076, 1973, 2005, 2335, 2515
RseI CAYNNNNRTG 2 cut(s) 652, 2175
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 4 cut(s) 636, 1014, 1518, 2211
SatI GCNGC 8 cut(s) 1401, 1539, 2481, 2535, 2598, 2601, 2604, 2630
Sau96I GGNCC 7 cut(s) 296, 365, 434, 503, 703, 2560, 2561
SchI GAGTC 6 cut(s) 31, 1341, 1400, 1485, 1601, 2304
SduI GDGCHC 4 cut(s) 127, 1121, 2564, 2617
SfaNI GCATC 6 cut(s) 926, 1182, 1249, 1964, 2142, 2419
SfcI CTRYAG 3 cut(s) 1122, 1683, 2478
SinI GGWCC 4 cut(s) 296, 365, 434, 503
SmaI CCCGGG 1 cut(s) 2508
SmiMI CAYNNNNRTG 2 cut(s) 652, 2175
SmlI CTYRAG 2 cut(s) 1720, 2189
SmoI CTYRAG 2 cut(s) 1720, 2189
SseBI AGGCCT 1 cut(s) 2621
SsiI CCGC 8 cut(s) 51, 1400, 1541, 1902, 1988, 2372, 2549, 2598
SspI AATATT 1 cut(s) 1471
SspMI CTAG 2 cut(s) 369, 987
StuI AGGCCT 1 cut(s) 2621
StyI CCWWGG 2 cut(s) 1968, 2402
TaaI ACNGT 9 cut(s) 422, 1050, 1229, 1448, 1516, 1680, 1775, 1946, 1963
TaiI ACGT 3 cut(s) 228, 801, 2076
TaqI TCGA 6 cut(s) 21, 399, 460, 784, 1765, 2313
TaqII GACCGA 1 cut(s) 152
TatI WGTACW 2 cut(s) 656, 2334
TauI GCSGC 2 cut(s) 1403, 2600
Tru1I TTAA 4 cut(s) 636, 1014, 1518, 2211
Tru9I TTAA 4 cut(s) 636, 1014, 1518, 2211
TscAI CASTG 7 cut(s) 427, 1150, 1234, 1606, 1692, 2242, 2311
TseFI GTSAC 2 cut(s) 228, 1487
TseI GCWGC 6 cut(s) 1538, 2480, 2534, 2600, 2603, 2629
Tsp45I GTSAC 2 cut(s) 228, 1487
TspGWI ACGGA 2 cut(s) 1536, 1767
TspMI CCCGGG 1 cut(s) 2506
TspRI CASTG 7 cut(s) 427, 1150, 1234, 1606, 1692, 2242, 2311
Tth111I GACNNNGTC 1 cut(s) 1908
Van91I CCANNNNNTGG 1 cut(s) 622
VpaK11BI GGWCC 4 cut(s) 296, 365, 434, 503
XapI RAATTY 4 cut(s) 715, 862, 1654, 2248
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 4 cut(s) 1994, 2128, 2446, 2500
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmaI CCCGGG 1 cut(s) 2506
XmiI GTMKAC 2 cut(s) 1912, 2070
XspI CTAG 2 cut(s) 369, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.