RLG00000020114

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005772
Physical Location & Seq
Forward (+)
61126860 .. 61135672
8813 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020114

Sequence Viewer

Length: 2757 bp
ATGGTCTCTGGGTCGCGAATCGAAGGTGGTGGGACTCAGATAATCTCCGCCGGAGTGAGGAAAACCATCCAATCCATCAAAGAAATAGTGGGCAATCACTCTGATATTGATATCTATTGGGCTCTCAAAGAGACCGATATGGATCCTAATGAAACTGCCCAGAAATTACTCAACCAAGACCCATTTCATGAGGTGAAGAGAAAAAGAGACAAAAAGAAGGACGTGTCACCTAAGAGTATCGGGCAAAAGGTTCCTACCGAGCCAAGAAGACATTTTGAGAGTGCTGGTCAAGGACCAAGATCTAATACAATTTTGGATCGTATTGTGGAGCCTAAAAGACATTTTGAGAGTGCAGGTCAAGGACCTAGATCAAATACAATTTCTGATCGTAATGTCGAGCCTAGAAGACATTTTGACAGTGCAGGTCAAGGGCCAAAATCTAATTCATTTTCTGATCGAAATGTTGAGCCAAGAAGACATTTTGAGAGTGCAGGTCAAGGACCAAGACATAATTCATTTTCTGATCGTAATGTTAGAAGAGGAGGTTATGTGCGCAGGGGTTTACCTGGAATCAGCAGAGAGTTTCGTGTTGTGAGAGACAACAGAGCTAACCAAAATATGGATGGAGAAATTAAGCCTGCTTCACCTCAATGTACTACATCCACCAACGAGCAAGTGATTTCATATGTTTCTGAGAAGGGCCAAACAGGAAATTCAAGCATTCAAAAGCCATCTAATAGACAGCATTCCTCACAAGCATTGAATGGGCAAACTGATTCTCGAATTAGAACGAACGTTGCTAATTCAACTGGCACTATTAGAAAGGAGACATTATTGGAGAAGCGAGTTGCTCTTCCAAATTCAGTTTCACGGGTACAAGCAGGGAAACCAAACAACTCCCAACCACATTCTGCATCAAGCACTTCTGTTATTGGGGTGTATTCCTCGTCCACAGATCCTGTTCATGTGCCATCTCCTGATTCTAGACCATCTGCTTCTGTTGGTGCAATTAAGCGCGAAGTTGGGGTGGTTGGTGTTCGGAAACAGTCTTCTGACAACTCCAAGTCAGTCGTACCAAGTAGTTCTTTCTCTAATTCACTTTTGGGAAAAGAGGGCTCTGCAGAATCATTTAGATCTTTCACTGGTATCTCTAAACCTGATCAACTCAGCCAAACTTCTGAATCTGTGATACCTAGCATGCCGGTCAGCAGATCTTTCGTAAGTAATCAGCACAATGTCAGGGCGCATCAACAACCTGTGGGTCATCAGAAAGATGCAGCTTCCCAGCCTAACAAGGAGTGGAAACCTAAATCAAGTCAGAAGCCAAGCCCTAATAGTCCTGGAGTCATTGGAACACCAACAAAATCTGCTTCTCCTCTTTCTGATGATTCAAAGGTCTCAGAGTCAGAAGCGGCTAAGTTGCAAGATAAGCTTGCCCGAGTAAATATATATGAGAACTGTAATGTTGTGATAGCGCAAAATATTAGAGTCCCTGAGAGTGACCGTTTTCGGCTAACCTTTGGAAGCTTGGGGACAGAGTTTGATTCAACAGGAAACACAGTTAACGGATTTCAAGCTGGAGCTGTGGAGGAGTTGAACAGGGAACCTCCAGCAAGTTTGTCAACATCAGCTCCCGAGTCGCGCAGTGATGAGGCTTCCGGCACGAAGCCAGTAGATTTATTAGATGACCAAGTTAGAAATTCTGGATCTGATTTTTCGGCACCGTCTGCAGTGCCTGAGCATTTGCCTGAGAAAAAAGAGACCTCAAGTCCTCAGAGTGTGGACAATTATGCAGATATTGGTTTGGTTCGAGACAACAGTCCGTCCTTTGCACCTTCAGATTCACAGCACCAAGATCCACCAGAGTTACAAGGTTTTACGGCATTTGATCCTCAGACTGGTTATGATATACCTTATTACAGACCCAGTATGGATGAAAGTGTTCGCGGGCAGGGTCTACCATCTCCTCAGGAGGCTTTGAGTTCACATAATGCCAACAGTATTCCTGCATCAACAGTTGCCATGGTACAACAGCAACCACCGCATGTGGCGCAGATGTACCCACAAGTTCATGTTTCACATTATGCTAATCTTATGCCATATCGTCAGTTTATCTCACCAGTATATGTTCCACCAATGGCTGTGCCTGGCTATTCCAATAATCCTGCCTATCCCCACATGTCTAATGGCAACAGCTACTTGCTGATGCCTGGAGGTGGTTCCCACCTAAATGCAAACAGCCTCAAGTATGGAGTTCAGCAATTTAAGCCCGTCCCTGCTGGAAGTCCCACTGGGTTTGGAAATTTTACTAACCCAGCAGGTTATGCAATGAATGCCCCTGGTGTAGTTGGAGGTGCCACTGGACTCGAAGATTCATCAAGAATGAAGTACAAAGATGGCAATCTTTATGTTCCAAATCCACAGGCGGAGACATCAGAGATTTGGATTCAGAACCCAAGGGAGCATCCAGGAATGCAATCCACTCCGTACTACAACATGCCTGGGCAAACACCTCATGCTCCTTATATGCCGTCCCACGCCAGCCACGCTTCCTTTAATGCAGCTGCAGCACAATCTTCGCACATGCAATTCCCGGGAATGTACCATCCTCCTCAGCCTGCCGCAATGGCCAGTCCGCACCATATGGGCCCTGCTATGCCCGGCAATGTCGGAGTGGGGGTGGCCGCAGCTGCTCCTGGGGCACAGGCGTATCAGCAGCCCCAGTTGAATCATATGAACTGGACAACAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

919

Amino Acids

99.23

Weight (kDa)

9.01

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 15 - 73 4.3e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 554
Acc36I ACCTGC 4 cut(s) 344, 413, 482, 2309
AccB1I GGYRCC 2 cut(s) 1720, 2354
AccB7I CCANNNNNTGG 1 cut(s) 619
AccI GTMKAC 1 cut(s) 1957
AccII CGCG 4 cut(s) 16, 1017, 1642, 1947
AciI CCGC 8 cut(s) 48, 1412, 1947, 2042, 2426, 2622, 2636, 2685
AclI AACGTT 1 cut(s) 795
AclWI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
AcoI YGGCCR 2 cut(s) 2628, 2682
AcsI RAATTY 4 cut(s) 712, 859, 1699, 2302
AcuI CTGAAG 1 cut(s) 1821
AfaI GTAC 8 cut(s) 655, 876, 1074, 2028, 2060, 2390, 2489, 2603
AfiI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
AflIII ACRYGT 2 cut(s) 222, 2178
AgsI TTSAA 9 cut(s) 717, 725, 763, 807, 1392, 1548, 1574, 1597, 2728
AhdI GACNNNNNGTC 1 cut(s) 1767
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 8 cut(s) 565, 1339, 2146, 2209, 2338, 2467, 2500, 2695
AjuI GAANNNNNNNTTGG 8 cut(s) 168, 200, 427, 459, 496, 528, 850, 882
Alw26I GTCTC 9 cut(s) 10, 125, 201, 591, 821, 1402, 1754, 1806, 2423
AlwI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
AlwNI CAGNNNCTG 2 cut(s) 959, 1736
Ama87I CYCGRG 3 cut(s) 1437, 1634, 2593
AoxI GGCC 5 cut(s) 431, 700, 2628, 2647, 2682
ApaI GGGCCC 1 cut(s) 2651
ApeKI GCWGC 7 cut(s) 1277, 2561, 2564, 2567, 2687, 2690, 2716
ApoI RAATTY 4 cut(s) 712, 859, 1699, 2302
Asp700I GAANNNNTTC 1 cut(s) 1941
AspLEI GCGC 6 cut(s) 555, 1017, 1246, 1477, 1644, 2053
AspS9I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
AsuC2I CCSGG 3 cut(s) 2594, 2595, 2661
AsuHPI GGTGA 4 cut(s) 205, 219, 636, 2109
AvaI CYCGRG 3 cut(s) 1437, 1634, 2593
AvaII GGWCC 3 cut(s) 293, 362, 500
AxyI CCTNAGG 1 cut(s) 1968
BaeGI GKGCMC 2 cut(s) 2651, 2704
BaeI ACNNNNGTAYC 2 cut(s) 2693, 2726
BalI TGGCCA 1 cut(s) 2630
BamHI GGATCC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 1720, 2354
BanII GRGCYC 3 cut(s) 124, 1118, 2651
BbsI GAAGAC 4 cut(s) 274, 412, 481, 1041
BbvCI CCTCAGC 1 cut(s) 2613
BbvI GCAGC 7 cut(s) 1289, 2551, 2573, 2579, 2677, 2699, 2728
BccI CCATC 9 cut(s) 74, 83, 617, 739, 979, 997, 1969, 2390, 2613
BceAI ACGGC 2 cut(s) 1896, 2515
BcgI CGANNNNNNTGC 4 cut(s) 1198, 1232, 2559, 2593
BciT130I CCWGG 8 cut(s) 567, 1341, 2148, 2211, 2340, 2469, 2502, 2697
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 3 cut(s) 2594, 2595, 2661
BcoDI GTCTC 9 cut(s) 10, 125, 201, 591, 821, 1402, 1754, 1806, 2423
BfaI CTAG 4 cut(s) 366, 402, 984, 1194
BfmI CTRYAG 3 cut(s) 1119, 1728, 2565
BfuAI ACCTGC 4 cut(s) 344, 413, 482, 2309
BglI GCCNNNNNGGC 1 cut(s) 2627
BglII AGATCT 3 cut(s) 299, 1133, 1211
Bme18I GGWCC 3 cut(s) 293, 362, 500
BmeRI GACNNNNNGTC 1 cut(s) 1767
BmeT110I CYCGRG 3 cut(s) 1437, 1634, 2593
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
BmiI GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
BmrI ACTGGG 3 cut(s) 1920, 2301, 2716
BmsI GCATC 6 cut(s) 923, 1255, 1264, 2018, 2196, 2473
BmuI ACTGGG 3 cut(s) 1920, 2301, 2716
BoxI GACNNNNGTC 1 cut(s) 1818
BpiI GAAGAC 4 cut(s) 274, 412, 481, 1041
BpmI CTGGAG 4 cut(s) 1362, 1593, 1599, 2232
Bpu10I CCTNAGC 2 cut(s) 1737, 2613
BpuEI CTTGAG 2 cut(s) 1750, 2228
BpuMI CCSGG 3 cut(s) 2594, 2595, 2661
BsaBI GATNNNNATC 2 cut(s) 141, 2400
BsaI GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsaJI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BsaXI ACNNNNNCTCC 6 cut(s) 534, 564, 1615, 1645, 2664, 2694
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
Bse118I RCCGGY 1 cut(s) 1201
Bse21I CCTNAGG 1 cut(s) 1968
Bse3DI GCAATG 3 cut(s) 2334, 2631, 2671
Bse8I GATNNNNATC 2 cut(s) 141, 2400
BseBI CCWGG 8 cut(s) 567, 1341, 2148, 2211, 2340, 2469, 2502, 2697
BseDI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BseGI GGATG 6 cut(s) 66, 628, 659, 1939, 2464, 2605
BseJI GATNNNNATC 2 cut(s) 141, 2400
BseLI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
BseMI GCAATG 3 cut(s) 2334, 2631, 2671
BseRI GAGGAG 5 cut(s) 555, 1365, 1604, 1956, 2601
BseSI GKGCMC 2 cut(s) 2651, 2704
BseXI GCAGC 7 cut(s) 1289, 2551, 2573, 2579, 2677, 2699, 2728
BseYI CCCAGC 2 cut(s) 1284, 2314
BsgI GTGCAG 3 cut(s) 372, 441, 510
Bsh1236I CGCG 4 cut(s) 16, 1017, 1642, 1947
BshFI GGCC 5 cut(s) 433, 702, 2630, 2649, 2684
BshNI GGYRCC 2 cut(s) 1720, 2354
BsiHKCI CYCGRG 3 cut(s) 1437, 1634, 2593
BsiSI CCGG 5 cut(s) 51, 1202, 1659, 2594, 2661
BslFI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
BslI CCNNNNNNNGG 4 cut(s) 57, 619, 1898, 2216
BsmAI GTCTC 9 cut(s) 10, 125, 201, 591, 821, 1402, 1754, 1806, 2423
BsmFI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
BsmI GAATGC 4 cut(s) 720, 745, 2338, 2478
BsnI GGCC 5 cut(s) 433, 702, 2630, 2649, 2684
Bso31I GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsoBI CYCGRG 3 cut(s) 1437, 1634, 2593
Bsp120I GGGCCC 1 cut(s) 2647
Bsp1286I GDGCHC 4 cut(s) 124, 1118, 2651, 2704
Bsp19I CCATGG 1 cut(s) 2022
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 8 cut(s) 48, 1412, 1947, 2042, 2426, 2622, 2636, 2685
BspANI GGCC 5 cut(s) 433, 702, 2630, 2649, 2684
BspFNI CGCG 4 cut(s) 16, 1017, 1642, 1947
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
BspMAI CTGCAG 3 cut(s) 1123, 1732, 2569
BspMI ACCTGC 4 cut(s) 344, 413, 482, 2309
BspPI GGATC 7 cut(s) 137, 150, 324, 950, 1714, 1850, 1883
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 1720, 2354
BspTNI GGTCTC 4 cut(s) 10, 125, 1402, 1754
BsrDI GCAATG 3 cut(s) 2334, 2631, 2671
BsrFI RCCGGY 1 cut(s) 1201
BssAI RCCGGY 1 cut(s) 1201
BssECI CCNNGG 6 cut(s) 2022, 2338, 2456, 2501, 2593, 2696
BssT1I CCWWGG 2 cut(s) 2022, 2456
Bst2UI CCWGG 8 cut(s) 567, 1341, 2148, 2211, 2340, 2469, 2502, 2697
Bst4CI ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
Bst6I CTCTTC 3 cut(s) 191, 532, 858
BstAPI GCANNNNNTGC 3 cut(s) 1727, 2324, 2333
BstC8I GCNNGC 6 cut(s) 639, 1199, 1434, 1949, 2542, 2619
BstDSI CCRYGG 1 cut(s) 2022
BstF5I GGATG 6 cut(s) 66, 628, 659, 1939, 2464, 2605
BstFNI CGCG 4 cut(s) 16, 1017, 1642, 1947
BstHHI GCGC 6 cut(s) 555, 1017, 1246, 1477, 1644, 2053
BstMAI GTCTC 9 cut(s) 10, 125, 201, 591, 821, 1402, 1754, 1806, 2423
BstNI CCWGG 8 cut(s) 567, 1341, 2148, 2211, 2340, 2469, 2502, 2697
BstNSI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
BstPAI GACNNNNGTC 1 cut(s) 1818
BstSFI CTRYAG 3 cut(s) 1119, 1728, 2565
BstSLI GKGCMC 2 cut(s) 2651, 2704
BstUI CGCG 4 cut(s) 16, 1017, 1642, 1947
BstV1I GCAGC 7 cut(s) 1289, 2551, 2573, 2579, 2677, 2699, 2728
BstV2I GAAGAC 4 cut(s) 274, 412, 481, 1041
BstX2I RGATCY 7 cut(s) 142, 299, 955, 1133, 1211, 1706, 1855
BstYI RGATCY 7 cut(s) 142, 299, 955, 1133, 1211, 1706, 1855
Bsu36I CCTNAGG 1 cut(s) 1968
BsuRI GGCC 5 cut(s) 433, 702, 2630, 2649, 2684
BtgI CCRYGG 1 cut(s) 2022
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 6 cut(s) 66, 628, 659, 1939, 2464, 2605
BtsI GCAGTG 2 cut(s) 1651, 1737
BtsIMutI CAGTG 6 cut(s) 424, 1140, 1651, 1737, 2289, 2358
BtuMI TCGCGA 1 cut(s) 16
BveI ACCTGC 4 cut(s) 344, 413, 482, 2309
Cac8I GCNNGC 6 cut(s) 639, 1199, 1434, 1949, 2542, 2619
CaiI CAGNNNCTG 2 cut(s) 959, 1736
CciI TCATGA 1 cut(s) 187
CfoI GCGC 6 cut(s) 555, 1017, 1246, 1477, 1644, 2053
Cfr10I RCCGGY 1 cut(s) 1201
Cfr13I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
Cfr9I CCCGGG 1 cut(s) 2593
Csp6I GTAC 8 cut(s) 654, 875, 1073, 2027, 2059, 2389, 2488, 2602
CspCI CAANNNNNGTGG 2 cut(s) 2278, 2313
CviQI GTAC 8 cut(s) 654, 875, 1073, 2027, 2059, 2389, 2488, 2602
DriI GACNNNNNGTC 1 cut(s) 1767
EaeI YGGCCR 2 cut(s) 2628, 2682
Eam1104I CTCTTC 3 cut(s) 191, 532, 858
Eam1105I GACNNNNNGTC 1 cut(s) 1767
EarI CTCTTC 3 cut(s) 191, 532, 858
EciI GGCGGA 2 cut(s) 37, 2441
Eco130I CCWWGG 2 cut(s) 2022, 2456
Eco24I GRGCYC 3 cut(s) 124, 1118, 2651
Eco31I GGTCTC 4 cut(s) 10, 125, 1402, 1754
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 3 cut(s) 293, 362, 500
Eco57I CTGAAG 1 cut(s) 1821
Eco81I CCTNAGG 1 cut(s) 1968
Eco88I CYCGRG 3 cut(s) 1437, 1634, 2593
EcoO109I RGGNCCY 2 cut(s) 362, 2648
EcoRII CCWGG 8 cut(s) 565, 1339, 2146, 2209, 2338, 2467, 2500, 2695
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 2022, 2456
EcoT38I GRGCYC 3 cut(s) 124, 1118, 2651
ErhI CCWWGG 2 cut(s) 2022, 2456
FalI AAGNNNNNCTT 4 cut(s) 1069, 1101, 1416, 1448
FaqI GGGAC 6 cut(s) 46, 1475, 1546, 2258, 2271, 2518
FauI CCCGC 1 cut(s) 1940
FauNDI CATATG 3 cut(s) 685, 2643, 2733
FbaI TGATCA 1 cut(s) 1159
FblI GTMKAC 1 cut(s) 1957
FokI GGATG 6 cut(s) 53, 635, 646, 1946, 2451, 2592
FriOI GRGCYC 3 cut(s) 124, 1118, 2651
FspBI CTAG 4 cut(s) 366, 402, 984, 1194
FspI TGCGCA 1 cut(s) 554
GlaI GCGC 6 cut(s) 554, 1016, 1245, 1476, 1643, 2052
GsaI CCCAGC 2 cut(s) 1288, 2318
GsuI CTGGAG 4 cut(s) 1362, 1593, 1599, 2232
HaeIII GGCC 5 cut(s) 433, 702, 2630, 2649, 2684
HapII CCGG 5 cut(s) 51, 1202, 1659, 2594, 2661
HhaI GCGC 6 cut(s) 555, 1017, 1246, 1477, 1644, 2053
Hin6I GCGC 6 cut(s) 553, 1015, 1244, 1475, 1642, 2051
HinP1I GCGC 6 cut(s) 553, 1015, 1244, 1475, 1642, 2051
HincII GTYRAC 2 cut(s) 1564, 1623
HindII GTYRAC 2 cut(s) 1564, 1623
HindIII AAGCTT 2 cut(s) 1430, 1525
HpaI GTTAAC 1 cut(s) 1564
HpaII CCGG 5 cut(s) 51, 1202, 1659, 2594, 2661
HphI GGTGA 4 cut(s) 205, 219, 636, 2109
Hpy166II GTNNAC 7 cut(s) 563, 951, 1564, 1623, 1783, 1958, 1985
Hpy8I GTNNAC 7 cut(s) 563, 951, 1564, 1623, 1783, 1958, 1985
HpyAV CCTTC 4 cut(s) 17, 211, 691, 1845
HpyCH4III ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
HpyCH4IV ACGT 2 cut(s) 222, 795
HpySE526I ACGT 2 cut(s) 222, 795
HspAI GCGC 6 cut(s) 553, 1015, 1244, 1475, 1642, 2051
Ksp22I TGATCA 1 cut(s) 1159
KspAI GTTAAC 1 cut(s) 1564
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 6 cut(s) 328, 1580, 1636, 2461, 2524, 2698
Lsp1109I GCAGC 7 cut(s) 1289, 2551, 2573, 2579, 2677, 2699, 2728
LweI GCATC 6 cut(s) 923, 1255, 1264, 2018, 2196, 2473
MaeI CTAG 4 cut(s) 366, 402, 984, 1194
MaeII ACGT 2 cut(s) 222, 795
MaeIII GTNAC 3 cut(s) 225, 1499, 1866
MboII GAAGA 9 cut(s) 208, 279, 417, 486, 549, 845, 1041, 2381, 2568
MflI RGATCY 7 cut(s) 142, 299, 955, 1133, 1211, 1706, 1855
MhlI GDGCHC 4 cut(s) 124, 1118, 2651, 2704
MlsI TGGCCA 1 cut(s) 2630
MluNI TGGCCA 1 cut(s) 2630
MlyI GAGTC 6 cut(s) 28, 1353, 1412, 1497, 1646, 2358
MmeI TCCRAC 2 cut(s) 2329, 2650
Mox20I TGGCCA 1 cut(s) 2630
MroXI GAANNNNTTC 1 cut(s) 1941
MscI TGGCCA 1 cut(s) 2630
MseI TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
MslI CAYNNNNRTG 2 cut(s) 649, 2229
Msp20I TGGCCA 1 cut(s) 2630
MspA1I CMGCKG 2 cut(s) 2564, 2690
MspI CCGG 5 cut(s) 51, 1202, 1659, 2594, 2661
Mva1269I GAATGC 4 cut(s) 720, 745, 2338, 2478
MvaI CCWGG 8 cut(s) 567, 1341, 2148, 2211, 2340, 2469, 2502, 2697
MvnI CGCG 4 cut(s) 16, 1017, 1642, 1947
NciI CCSGG 3 cut(s) 2594, 2595, 2661
NcoI CCATGG 1 cut(s) 2022
NdeI CATATG 3 cut(s) 685, 2643, 2733
NlaIV GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
NmuCI GTSAC 2 cut(s) 225, 1499
NruI TCGCGA 1 cut(s) 16
NsbI TGCGCA 1 cut(s) 554
NspI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
PaeI GCATGC 1 cut(s) 1201
PagI TCATGA 1 cut(s) 187
PciI ACATGT 1 cut(s) 2178
PciSI GCTCTTC 1 cut(s) 858
PctI GAATGC 4 cut(s) 720, 745, 2338, 2478
PdmI GAANNNNTTC 1 cut(s) 1941
PflMI CCANNNNNTGG 1 cut(s) 619
PfoI TCCNGGA 2 cut(s) 1339, 2467
PleI GAGTC 6 cut(s) 28, 1352, 1411, 1496, 1645, 2358
PpsI GAGTC 6 cut(s) 28, 1352, 1411, 1496, 1645, 2358
PpuMI RGGWCCY 1 cut(s) 362
PscI ACATGT 1 cut(s) 2178
PshAI GACNNNNGTC 1 cut(s) 1818
Psp1406I AACGTT 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 8 cut(s) 565, 1339, 2146, 2209, 2338, 2467, 2500, 2695
PspFI CCCAGC 2 cut(s) 1284, 2314
PspGI CCWGG 8 cut(s) 565, 1339, 2146, 2209, 2338, 2467, 2500, 2695
PspN4I GGNNCC 8 cut(s) 144, 252, 330, 1605, 1722, 2221, 2356, 2649
PspOMI GGGCCC 1 cut(s) 2647
PspPI GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
PspPPI RGGWCCY 1 cut(s) 362
PstI CTGCAG 3 cut(s) 1123, 1732, 2569
PstNI CAGNNNCTG 2 cut(s) 959, 1736
PsuI RGATCY 7 cut(s) 142, 299, 955, 1133, 1211, 1706, 1855
PvuII CAGCTG 2 cut(s) 2564, 2690
RruI TCGCGA 1 cut(s) 16
RsaI GTAC 8 cut(s) 655, 876, 1074, 2028, 2060, 2390, 2489, 2603
RsaNI GTAC 8 cut(s) 654, 875, 1073, 2027, 2059, 2389, 2488, 2602
RseI CAYNNNNRTG 2 cut(s) 649, 2229
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
Sau96I GGNCC 7 cut(s) 293, 362, 431, 500, 700, 2647, 2648
SchI GAGTC 6 cut(s) 28, 1353, 1412, 1497, 1646, 2358
SduI GDGCHC 4 cut(s) 124, 1118, 2651, 2704
SfaNI GCATC 6 cut(s) 923, 1255, 1264, 2018, 2196, 2473
SfcI CTRYAG 3 cut(s) 1119, 1728, 2565
SinI GGWCC 3 cut(s) 293, 362, 500
SmaI CCCGGG 1 cut(s) 2595
SmiMI CAYNNNNRTG 2 cut(s) 649, 2229
SmlI CTYRAG 2 cut(s) 1765, 2243
SmoI CTYRAG 2 cut(s) 1765, 2243
SphI GCATGC 1 cut(s) 1201
SsiI CCGC 8 cut(s) 48, 1412, 1947, 2042, 2426, 2622, 2636, 2685
SspI AATATT 1 cut(s) 1483
SspMI CTAG 4 cut(s) 366, 402, 984, 1194
StyI CCWWGG 2 cut(s) 2022, 2456
TaaI ACNGT 9 cut(s) 419, 1047, 1460, 1505, 1561, 1725, 1820, 2000, 2017
TaiI ACGT 2 cut(s) 225, 798
TaqI TCGA 6 cut(s) 21, 396, 457, 781, 1810, 2367
TaqII GACCGA 1 cut(s) 149
TatI WGTACW 2 cut(s) 653, 2388
TauI GCSGC 3 cut(s) 1415, 2624, 2687
Tru1I TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
Tru9I TTAA 5 cut(s) 633, 1011, 1563, 2265, 2556
TscAI CASTG 6 cut(s) 424, 1147, 1651, 1737, 2296, 2365
TseFI GTSAC 2 cut(s) 225, 1499
TseI GCWGC 7 cut(s) 1277, 2561, 2564, 2567, 2687, 2690, 2716
Tsp45I GTSAC 2 cut(s) 225, 1499
TspGWI ACGGA 3 cut(s) 1581, 1812, 2475
TspMI CCCGGG 1 cut(s) 2593
TspRI CASTG 6 cut(s) 424, 1147, 1651, 1737, 2296, 2365
Van91I CCANNNNNTGG 1 cut(s) 619
VpaK11BI GGWCC 3 cut(s) 293, 362, 500
XapI RAATTY 4 cut(s) 712, 859, 1699, 2302
XbaI TCTAGA 1 cut(s) 983
XceI RCATGY 5 cut(s) 1201, 2048, 2182, 2500, 2587
XcmI CCANNNNNNNNNTGG 2 cut(s) 620, 2183
XmaI CCCGGG 1 cut(s) 2593
XmiI GTMKAC 1 cut(s) 1957
XmnI GAANNNNTTC 1 cut(s) 1941
XspI CTAG 4 cut(s) 366, 402, 984, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.