MD09G1185800.v1.1

Protein of unknown function (DUF1296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005772
Physical Location & Seq
Reverse (-)
16099874 .. 16108156
8283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1185800.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGGTCGCCGGGTCGCGAATCGAAGGTGGGACTCAGATAATCTCAGCAGGAGTGAGAAAAACGATTCAATCGATCAAAGAAATAGTGGGTAATTACTCGGATATTGATATCTATTGGGCTCTCAAAGAAACCGACATGGATCCTAATGAAACGGCCCAGAAATTGCTCAACCAAGACCCATTTCATGTTGTGAAGAGAAAAAGAGACAAAAGGAAGGAGGTATCCTCTAATAGTAATGGGCACAAGGTTTCTGTGGATGCAAGGAGACATTCTGAGAGTGCAGGCCAAGGACCCAAACCAAATACATCCTCTGATCGCCCCTCTTCTCAGAATACAACTTCTGATCATCCCTCTTCTCAGAATACGTCTGATCGTCCTTCATCTCACAATACATTTCCTGATCGTCCCTCATCTCGCAATACATTTTCTGATCATCCCTCATCTCGCAATACATTTTCTGATCGTAATGTTAGAAGAGGAGGTTATGCTCGTGGCGGTTCAACTGGTACAAGAATCAGCAGAGAATTCCGTGTTGTGAGAGACAACCGAATTAACGGTAATGTCAATAAAGAAGCGAAGCCTGCTTCACCAAAATGTACAACTTCCACCAATGAGCAAGCGTCAAATAATTCTGGAAAGGGCCCAACAGGAAGTTCAAATAGTCAAAAGCCATCTAGCAGACAAAATTCATCTCATGGATTGAATGCGCAAACTCAAATTAGAAGCAGTGGTGCTAATTCAACTGGTACTGTTAGAAAAGAGACATTAGTGGAGAACCGAGTTACTCTTCCAAATGTAGCTTCACGGGGGCAAGGAGGGAAGCCAAACAGTTCCCAACTGCACTCTCCAATGGTGATATCGAGCAACTCTGTTGTTGGGGTGTATTCATCTTCCACGGATCCTGTTCATGTGCCATCTCCTGATTCCAGACCAGCTGCTTCTGTTGGTGCTATTAAACGGGAGGTTGGTGTTAGGAGACAAGCTTCTGACAGCTCCAAGTCCTTTGCTCCAAGTAGCTCTGTGACTTCTGTCTCAAATTCACTTTTGGGAAAAGAGGATTCTACTGAGTCATTTCGACCTTTTACTGGAATCTCTAAAACTGATCAAGTGAGCCAAACTTCTGAATCTGTGATACCTAGCATGTCGGGCAGCAGACCGTTGTTAAGTAATCAGCACAATGTCAGGCCACACCAACAACCTGTGGGACATCAAAAAGCTTCCCAGCCTAATAAGGAGTGGAAACCTAAGTCAAGCCAAAAGGCAAGTTCTAATAGTCCTGGAGTCATTGGAACACCAACAAAATCTGTTTCACCTGCTTCTGGTGATCCTCAGGTTTCAGAGTCAGAAGCGGCTAAATTGCAAGATAAGCTCGCTCTAGTAAATATATATGATAACTGTAACGTTGTCATAGCACATAGTATTAGGGTCCCAGACAGTGATCGTTTTCAGTTAACATTTGGAAGCTTGGGGACAGAGCCTGATTCAAGAGGGAACATAGTTAATGGATTTCAAGTGGGAGCTTCAGAGGAGTCAAACAGGGAAGCTGCAGCAAGTTTGTCAGTATCAGCTGCAGAGTCGTGCAGTGATGAGGCTTCTGCCATAAATCCGGTGGATTTATTAGATCACCAAGTAAGTTCTGGATCTGATTCCCCTGCATCAGGTGTAGTTGCTGAACATCAATTGCCTGAGAATAAAGAGACTTTGAGTCCTCAGAACTTGGGCAATTATGCAGATATTGGTTTGGTTCAAGACAACAGTTCATCCTATGCACCTTCCGACTCACAGCAGCAAGATCCACCAGAGTTACAAGGTTTTTCGGCATTTGATCCTCAGACTGGTTATGACATGCCGTATTTCAGACCACATATGGAGGAAAATCGTGGACAGGGTCTACCTCCTCCTCAAGAGGCCTTGAGCTCACATACTATCAACAGCATGGCTGCATCAACAGTTGCCATGGTACAACAGCAACCACCCCCAGTGGCGCAGATGTACCCACAAGTTCATGTTTCACATTATGCTAATCTTATGCCATATCGCCAATTTCTCTCACCAGTTTATGTTCCACCAATGGCTGTGCCTGGCTATTCCAACAACCCTGCGTATCCTCACCTGTCTAATGGCAGCAGCTACTTGCTGATGCCTGGAGGTGGTTCACACCTAAATGCAAACAGCCTCAAGTACGGAGTGCAGCAGTTTAAGCCTGCCCCTGCTGGAAGTCCCACAGGGTTTGGGAATTTTACGAATCCCAATGGGTATGCAATGAACGCCCCTGGTGTTGTTGGAGGGGCCACTGGGCTAGAAGATTCATCTCGAATCAAGTACAAAGATGGCAATCTATACATCCCAAATCCTCAGGCCGAGACATCGGAGATTTGGATTCAGAACGCAAGGGAGCACCCGGGCATGCAATCCACTCCGTACTACAACATGCCTGCACAATCGCCTCACGGTGCTTACATGCCATCTCACGCTACTCATGCCTCCTTCAATGCAGCTGCGGCCCAATCTTCTCACATGCAGTTCCCAGGTTTGTACCATCCTCCTCAGCCTACCGCAATTCCCAACGCGCACCATATGGGACCTGCTATGGGCGGTAATGTCGGTGTTGGGGTGGCTGCAGCGTCTCCTGGGGCACAAGTTGGTGCGTATCAGCAACCCCAACTGAATCATATGAATTGGCAATCGAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1296

Protein Analysis

899

Amino Acids

95.74

Weight (kDa)

8.6

Isoelectric Point (pI)

56.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 14 - 73 5.8e-29 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1321
Acc16I TGCGCA 1 cut(s) 708
Acc36I ACCTGC 2 cut(s) 1321, 2593
AccI GTMKAC 1 cut(s) 1891
AccII CGCG 2 cut(s) 16, 2570
AciI CCGC 5 cut(s) 495, 1349, 2501, 2556, 2595
AclI AACGTT 1 cut(s) 1401
AclWI GGATC 8 cut(s) 134, 147, 893, 906, 1319, 1648, 1787, 1820
AcsI RAATTY 4 cut(s) 524, 685, 1036, 2236
AcuI CTGAAG 1 cut(s) 1506
AfaI GTAC 9 cut(s) 508, 598, 748, 1962, 1994, 2183, 2324, 2423, 2537
AfiI CCNNNNNNNGG 6 cut(s) 1085, 1319, 1658, 1835, 2150, 2591
AgsI TTSAA 9 cut(s) 68, 501, 657, 703, 741, 1485, 1511, 1748, 2491
AhdI GACNNNNNGTC 1 cut(s) 1704
AjnI CCWGG 6 cut(s) 1276, 2080, 2143, 2272, 2527, 2629
AjuI GAANNNNNNNTTGG 6 cut(s) 165, 197, 637, 669, 784, 816
Alw21I GWGCWC 2 cut(s) 1919, 2400
Alw26I GTCTC 9 cut(s) 198, 259, 534, 755, 970, 1036, 1691, 2357, 2631
AlwI GGATC 8 cut(s) 134, 147, 893, 906, 1319, 1648, 1787, 1820
AlwNI CAGNNNCTG 1 cut(s) 1478
Ama87I CYCGRG 1 cut(s) 2401
AoxI GGCC 8 cut(s) 153, 283, 640, 1184, 1908, 2289, 2358, 2502
ApaI GGGCCC 1 cut(s) 644
ApoI RAATTY 4 cut(s) 524, 685, 1036, 2236
AspLEI GCGC 3 cut(s) 709, 1987, 2572
AspS9I GGNCC 8 cut(s) 154, 290, 640, 641, 1426, 2289, 2503, 2582
AsuC2I CCSGG 3 cut(s) 10, 2402, 2403
AsuHPI GGTGA 7 cut(s) 579, 865, 1302, 1334, 1616, 2043, 2102
AvaI CYCGRG 1 cut(s) 2401
AvaII GGWCC 3 cut(s) 290, 1426, 2582
AxyI CCTNAGG 2 cut(s) 1329, 2355
BaeGI GKGCMC 3 cut(s) 243, 644, 2638
BamHI GGATCC 2 cut(s) 139, 898
BanII GRGCYC 3 cut(s) 121, 644, 1919
BauI CACGAG 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 1919, 2400
BbvCI CCTCAGC 1 cut(s) 2547
BccI CCATC 5 cut(s) 679, 922, 2324, 2473, 2547
BceAI ACGGC 2 cut(s) 168, 1834
BciT130I CCWGG 6 cut(s) 1278, 2082, 2145, 2274, 2529, 2631
BciVI GTATCC 2 cut(s) 232, 2115
BclI TGATCA 3 cut(s) 343, 430, 1102
BcnI CCSGG 3 cut(s) 10, 2402, 2403
BcoDI GTCTC 9 cut(s) 198, 259, 534, 755, 970, 1036, 1691, 2357, 2631
BfaI CTAG 4 cut(s) 675, 1137, 1376, 2300
BfmI CTRYAG 3 cut(s) 1545, 1569, 2619
BfuAI ACCTGC 2 cut(s) 1321, 2593
BfuI GTATCC 2 cut(s) 232, 2115
Bme1390I CCNGG 9 cut(s) 10, 1278, 2082, 2145, 2274, 2402, 2403, 2529, 2631
Bme18I GGWCC 3 cut(s) 290, 1426, 2582
BmeRI GACNNNNNGTC 1 cut(s) 1704
BmeT110I CYCGRG 1 cut(s) 2401
BmgT120I GGNCC 8 cut(s) 154, 290, 640, 641, 1426, 2289, 2503, 2582
BmiI GGNNCC 8 cut(s) 141, 292, 642, 900, 1427, 1428, 2290, 2583
BmrFI CCNGG 9 cut(s) 10, 1278, 2082, 2145, 2274, 2402, 2403, 2529, 2631
BmrI ACTGGG 2 cut(s) 1973, 2304
BmsI GCATC 4 cut(s) 247, 1664, 1952, 2130
BmuI ACTGGG 2 cut(s) 1973, 2304
BoxI GACNNNNGTC 1 cut(s) 1028
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 2 cut(s) 1299, 2166
Bpu10I CCTNAGC 1 cut(s) 2547
BpuEI CTTGAG 3 cut(s) 1887, 1933, 2162
BpuMI CCSGG 3 cut(s) 10, 2402, 2403
Bsa29I ATCGAT 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 2334
BsaJI CCNNGG 7 cut(s) 286, 894, 1956, 2272, 2401, 2527, 2630
BsaWI WCCGGW 1 cut(s) 1606
Bsc4I CCNNNNNNNGG 6 cut(s) 1085, 1319, 1658, 1835, 2150, 2591
Bse1I ACTGG 7 cut(s) 508, 748, 1090, 1840, 1979, 2054, 2299
Bse21I CCTNAGG 2 cut(s) 1329, 2355
Bse3DI GCAATG 1 cut(s) 2268
Bse8I GATNNNNATC 1 cut(s) 2334
BseBI CCWGG 6 cut(s) 1278, 2082, 2145, 2274, 2529, 2631
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 7 cut(s) 286, 894, 1956, 2272, 2401, 2527, 2630
BseGI GGATG 7 cut(s) 262, 305, 346, 433, 1760, 2343, 2539
BseJI GATNNNNATC 1 cut(s) 2334
BseLI CCNNNNNNNGG 6 cut(s) 1085, 1319, 1658, 1835, 2150, 2591
BseMI GCAATG 1 cut(s) 2268
BseNI ACTGG 7 cut(s) 508, 748, 1090, 1840, 1979, 2054, 2299
BseRI GAGGAG 5 cut(s) 492, 1541, 1887, 1890, 2535
BseSI GKGCMC 3 cut(s) 243, 644, 2638
BseYI CCCAGC 1 cut(s) 1221
BsgI GTGCAG 5 cut(s) 300, 824, 1600, 2210, 2421
Bsh1236I CGCG 2 cut(s) 16, 2570
BshFI GGCC 8 cut(s) 155, 285, 642, 1186, 1910, 2291, 2360, 2504
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 1919, 2400
BsiHKCI CYCGRG 1 cut(s) 2401
BsiSI CCGG 3 cut(s) 9, 1607, 2402
BslFI GGGAC 7 cut(s) 43, 390, 1218, 1412, 1483, 2205, 2595
BslI CCNNNNNNNGG 6 cut(s) 1085, 1319, 1658, 1835, 2150, 2591
BsmAI GTCTC 9 cut(s) 198, 259, 534, 755, 970, 1036, 1691, 2357, 2631
BsmBI CGTCTC 1 cut(s) 2631
BsmFI GGGAC 7 cut(s) 43, 390, 1218, 1412, 1483, 2205, 2595
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 8 cut(s) 155, 285, 642, 1186, 1910, 2291, 2360, 2504
BsoBI CYCGRG 1 cut(s) 2401
Bsp120I GGGCCC 1 cut(s) 640
Bsp1286I GDGCHC 6 cut(s) 121, 243, 644, 1919, 2400, 2638
Bsp1407I TGTACA 1 cut(s) 596
Bsp19I CCATGG 1 cut(s) 1956
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 5 cut(s) 495, 1349, 2501, 2556, 2595
BspANI GGCC 8 cut(s) 155, 285, 642, 1186, 1910, 2291, 2360, 2504
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 2 cut(s) 16, 2570
BspLI GGNNCC 8 cut(s) 141, 292, 642, 900, 1427, 1428, 2290, 2583
BspMAI CTGCAG 3 cut(s) 1549, 1573, 2623
BspMI ACCTGC 2 cut(s) 1321, 2593
BspPI GGATC 8 cut(s) 134, 147, 893, 906, 1319, 1648, 1787, 1820
BsrDI GCAATG 1 cut(s) 2268
BsrGI TGTACA 1 cut(s) 596
BsrI ACTGG 7 cut(s) 508, 748, 1090, 1840, 1979, 2054, 2299
BssECI CCNNGG 7 cut(s) 286, 894, 1956, 2272, 2401, 2527, 2630
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 286, 1956
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 6 cut(s) 1278, 2082, 2145, 2274, 2529, 2631
Bst4CI ACNGT 9 cut(s) 557, 751, 830, 1158, 1397, 1436, 1757, 1951, 2453
Bst6I CTCTTC 5 cut(s) 188, 328, 358, 469, 792
BstAUI TGTACA 1 cut(s) 596
BstC8I GCNNGC 7 cut(s) 283, 582, 618, 1371, 2205, 2408, 2436
BstDSI CCRYGG 2 cut(s) 894, 1956
BstENI CCTNNNNNAGG 1 cut(s) 1656
BstF5I GGATG 7 cut(s) 262, 305, 346, 433, 1760, 2343, 2539
BstFNI CGCG 2 cut(s) 16, 2570
BstHHI GCGC 3 cut(s) 709, 1987, 2572
BstMAI GTCTC 9 cut(s) 198, 259, 534, 755, 970, 1036, 1691, 2357, 2631
BstMWI GCNNNNNNNGC 7 cut(s) 581, 1146, 1366, 2200, 2267, 2480, 2501
BstNI CCWGG 6 cut(s) 1278, 2082, 2145, 2274, 2529, 2631
BstNSI RCATGY 6 cut(s) 1144, 1849, 2410, 2434, 2464, 2521
BstPAI GACNNNNGTC 1 cut(s) 1028
BstSCI CCNGG 9 cut(s) 8, 1276, 2080, 2143, 2272, 2400, 2401, 2527, 2629
BstSFI CTRYAG 3 cut(s) 1545, 1569, 2619
BstSLI GKGCMC 3 cut(s) 243, 644, 2638
BstUI CGCG 2 cut(s) 16, 2570
BstX2I RGATCY 4 cut(s) 139, 898, 1640, 1792
BstYI RGATCY 4 cut(s) 139, 898, 1640, 1792
Bsu15I ATCGAT 1 cut(s) 71
Bsu36I CCTNAGG 2 cut(s) 1329, 2355
BsuI GTATCC 2 cut(s) 232, 2115
BsuRI GGCC 8 cut(s) 155, 285, 642, 1186, 1910, 2291, 2360, 2504
BsuTUI ATCGAT 1 cut(s) 71
BtgI CCRYGG 2 cut(s) 894, 1956
BtsCI GGATG 7 cut(s) 262, 305, 346, 433, 1760, 2343, 2539
BtsI GCAGTG 2 cut(s) 733, 1588
BtsIMutI CAGTG 5 cut(s) 733, 1441, 1588, 1986, 2292
BtuMI TCGCGA 1 cut(s) 16
BveI ACCTGC 2 cut(s) 1321, 2593
Cac8I GCNNGC 7 cut(s) 283, 582, 618, 1371, 2205, 2408, 2436
CaiI CAGNNNCTG 1 cut(s) 1478
CfoI GCGC 3 cut(s) 709, 1987, 2572
Cfr13I GGNCC 8 cut(s) 154, 290, 640, 641, 1426, 2289, 2503, 2582
Cfr9I CCCGGG 1 cut(s) 2401
ClaI ATCGAT 1 cut(s) 71
CseI GACGC 2 cut(s) 609, 2613
Csp6I GTAC 9 cut(s) 507, 597, 747, 1961, 1993, 2182, 2323, 2422, 2536
CspCI CAANNNNNGTGG 2 cut(s) 2212, 2247
CviQI GTAC 9 cut(s) 507, 597, 747, 1961, 1993, 2182, 2323, 2422, 2536
DriI GACNNNNNGTC 1 cut(s) 1704
Eam1104I CTCTTC 5 cut(s) 188, 328, 358, 469, 792
Eam1105I GACNNNNNGTC 1 cut(s) 1704
EarI CTCTTC 5 cut(s) 188, 328, 358, 469, 792
Ecl136II GAGCTC 1 cut(s) 1917
Eco130I CCWWGG 2 cut(s) 286, 1956
Eco147I AGGCCT 1 cut(s) 1910
Eco24I GRGCYC 3 cut(s) 121, 644, 1919
Eco32I GATATC 2 cut(s) 109, 858
Eco47I GGWCC 3 cut(s) 290, 1426, 2582
Eco53kI GAGCTC 1 cut(s) 1917
Eco57I CTGAAG 1 cut(s) 1506
Eco81I CCTNAGG 2 cut(s) 1329, 2355
Eco88I CYCGRG 1 cut(s) 2401
EcoICRI GAGCTC 1 cut(s) 1917
EcoNI CCTNNNNNAGG 1 cut(s) 1656
EcoO109I RGGNCCY 4 cut(s) 290, 640, 1426, 2582
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 6 cut(s) 1276, 2080, 2143, 2272, 2527, 2629
EcoRV GATATC 2 cut(s) 109, 858
EcoT14I CCWWGG 2 cut(s) 286, 1956
EcoT38I GRGCYC 3 cut(s) 121, 644, 1919
ErhI CCWWGG 2 cut(s) 286, 1956
Esp3I CGTCTC 1 cut(s) 2631
FaqI GGGAC 7 cut(s) 43, 390, 1218, 1412, 1483, 2205, 2595
FauNDI CATATG 3 cut(s) 1866, 2577, 2673
FbaI TGATCA 3 cut(s) 343, 430, 1102
FblI GTMKAC 1 cut(s) 1891
FokI GGATG 7 cut(s) 269, 292, 333, 420, 1747, 2330, 2526
FriOI GRGCYC 3 cut(s) 121, 644, 1919
FspBI CTAG 4 cut(s) 675, 1137, 1376, 2300
FspI TGCGCA 1 cut(s) 708
GlaI GCGC 3 cut(s) 708, 1986, 2571
GsaI CCCAGC 1 cut(s) 1225
GsuI CTGGAG 2 cut(s) 1299, 2166
HaeIII GGCC 8 cut(s) 155, 285, 642, 1186, 1910, 2291, 2360, 2504
HapII CCGG 3 cut(s) 9, 1607, 2402
HgaI GACGC 2 cut(s) 609, 2613
HhaI GCGC 3 cut(s) 709, 1987, 2572
Hin6I GCGC 3 cut(s) 707, 1985, 2570
HinP1I GCGC 3 cut(s) 707, 1985, 2570
HincII GTYRAC 1 cut(s) 1452
HindII GTYRAC 1 cut(s) 1452
HindIII AAGCTT 3 cut(s) 981, 1215, 1462
HpaI GTTAAC 1 cut(s) 1452
HpaII CCGG 3 cut(s) 9, 1607, 2402
HphI GGTGA 7 cut(s) 579, 865, 1302, 1334, 1616, 2043, 2102
Hpy166II GTNNAC 4 cut(s) 1452, 1883, 1892, 2156
Hpy8I GTNNAC 4 cut(s) 1452, 1883, 1892, 2156
HpyAV CCTTC 5 cut(s) 17, 208, 387, 1782, 2497
HpyCH4III ACNGT 9 cut(s) 557, 751, 830, 1158, 1397, 1436, 1757, 1951, 2453
HpyCH4IV ACGT 2 cut(s) 365, 1401
HpyF10VI GCNNNNNNNGC 7 cut(s) 581, 1146, 1366, 2200, 2267, 2480, 2501
HpySE526I ACGT 2 cut(s) 365, 1401
HspAI GCGC 3 cut(s) 707, 1985, 2570
KflI GGGWCCC 1 cut(s) 1426
Ksp22I TGATCA 3 cut(s) 343, 430, 1102
KspAI GTTAAC 1 cut(s) 1452
LmnI GCTCC 4 cut(s) 998, 1012, 1517, 2395
LweI GCATC 4 cut(s) 247, 1664, 1952, 2130
MaeI CTAG 4 cut(s) 675, 1137, 1376, 2300
MaeII ACGT 2 cut(s) 365, 1401
MaeIII GTNAC 4 cut(s) 781, 1021, 1397, 1803
MboII GAAGA 8 cut(s) 205, 315, 345, 486, 779, 882, 2315, 2502
MfeI CAATTG 1 cut(s) 1679
MflI RGATCY 4 cut(s) 139, 898, 1640, 1792
MhlI GDGCHC 6 cut(s) 121, 243, 644, 1919, 2400, 2638
MlyI GAGTC 8 cut(s) 25, 1076, 1290, 1349, 1538, 1583, 1714, 1772
MmeI TCCRAC 3 cut(s) 1800, 2115, 2263
MseI TTAA 6 cut(s) 552, 954, 1163, 1451, 1500, 2199
MslI CAYNNNNRTG 2 cut(s) 592, 2163
MspA1I CMGCKG 3 cut(s) 935, 1568, 2498
MspI CCGG 3 cut(s) 9, 1607, 2402
MspR9I CCNGG 9 cut(s) 10, 1278, 2082, 2145, 2274, 2402, 2403, 2529, 2631
MunI CAATTG 1 cut(s) 1679
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 6 cut(s) 1278, 2082, 2145, 2274, 2529, 2631
MvnI CGCG 2 cut(s) 16, 2570
MwoI GCNNNNNNNGC 7 cut(s) 581, 1146, 1366, 2200, 2267, 2480, 2501
NciI CCSGG 3 cut(s) 10, 2402, 2403
NcoI CCATGG 1 cut(s) 1956
NdeI CATATG 3 cut(s) 1866, 2577, 2673
NlaIV GGNNCC 8 cut(s) 141, 292, 642, 900, 1427, 1428, 2290, 2583
NmeAIII GCCGAG 1 cut(s) 2386
NmuCI GTSAC 1 cut(s) 1021
NruI TCGCGA 1 cut(s) 16
NsbI TGCGCA 1 cut(s) 708
NspI RCATGY 6 cut(s) 1144, 1849, 2410, 2434, 2464, 2521
PaeI GCATGC 1 cut(s) 2410
PaqCI CACCTGC 1 cut(s) 1321
PceI AGGCCT 1 cut(s) 1910
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PctI GAATGC 1 cut(s) 709
PflFI GACNNNGTC 1 cut(s) 1887
PfoI TCCNGGA 1 cut(s) 1276
PleI GAGTC 8 cut(s) 25, 1075, 1289, 1348, 1537, 1582, 1713, 1772
PpsI GAGTC 8 cut(s) 25, 1075, 1289, 1348, 1537, 1582, 1713, 1772
PpuMI RGGWCCY 3 cut(s) 290, 1426, 2582
PshAI GACNNNNGTC 1 cut(s) 1028
Psp124BI GAGCTC 1 cut(s) 1919
Psp1406I AACGTT 1 cut(s) 1401
Psp5II RGGWCCY 3 cut(s) 290, 1426, 2582
Psp6I CCWGG 6 cut(s) 1276, 2080, 2143, 2272, 2527, 2629
PspFI CCCAGC 1 cut(s) 1221
PspGI CCWGG 6 cut(s) 1276, 2080, 2143, 2272, 2527, 2629
PspN4I GGNNCC 8 cut(s) 141, 292, 642, 900, 1427, 1428, 2290, 2583
PspOMI GGGCCC 1 cut(s) 640
PspPI GGNCC 8 cut(s) 154, 290, 640, 641, 1426, 2289, 2503, 2582
PspPPI RGGWCCY 3 cut(s) 290, 1426, 2582
PstI CTGCAG 3 cut(s) 1549, 1573, 2623
PstNI CAGNNNCTG 1 cut(s) 1478
PsuI RGATCY 4 cut(s) 139, 898, 1640, 1792
PsyI GACNNNGTC 1 cut(s) 1887
PvuII CAGCTG 3 cut(s) 935, 1568, 2498
RruI TCGCGA 1 cut(s) 16
RsaI GTAC 9 cut(s) 508, 598, 748, 1962, 1994, 2183, 2324, 2423, 2537
RsaNI GTAC 9 cut(s) 507, 597, 747, 1961, 1993, 2182, 2323, 2422, 2536
RseI CAYNNNNRTG 2 cut(s) 592, 2163
SacI GAGCTC 1 cut(s) 1919
SaqAI TTAA 6 cut(s) 552, 954, 1163, 1451, 1500, 2199
Sau96I GGNCC 8 cut(s) 154, 290, 640, 641, 1426, 2289, 2503, 2582
SchI GAGTC 8 cut(s) 25, 1076, 1290, 1349, 1538, 1583, 1714, 1772
ScrFI CCNGG 9 cut(s) 10, 1278, 2082, 2145, 2274, 2402, 2403, 2529, 2631
SduI GDGCHC 6 cut(s) 121, 243, 644, 1919, 2400, 2638
SfaNI GCATC 4 cut(s) 247, 1664, 1952, 2130
SfcI CTRYAG 3 cut(s) 1545, 1569, 2619
SinI GGWCC 3 cut(s) 290, 1426, 2582
SmaI CCCGGG 1 cut(s) 2403
SmiMI CAYNNNNRTG 2 cut(s) 592, 2163
SmlI CTYRAG 3 cut(s) 1902, 1912, 2177
SmoI CTYRAG 3 cut(s) 1902, 1912, 2177
SphI GCATGC 1 cut(s) 2410
SseBI AGGCCT 1 cut(s) 1910
SsiI CCGC 5 cut(s) 495, 1349, 2501, 2556, 2595
SspMI CTAG 4 cut(s) 675, 1137, 1376, 2300
SstI GAGCTC 1 cut(s) 1919
StuI AGGCCT 1 cut(s) 1910
StyD4I CCNGG 9 cut(s) 8, 1276, 2080, 2143, 2272, 2400, 2401, 2527, 2629
StyI CCWWGG 2 cut(s) 286, 1956
TaaI ACNGT 9 cut(s) 557, 751, 830, 1158, 1397, 1436, 1757, 1951, 2453
TaiI ACGT 2 cut(s) 368, 1404
TaqI TCGA 6 cut(s) 21, 71, 860, 1075, 2314, 2687
TatI WGTACW 2 cut(s) 596, 2322
TauI GCSGC 2 cut(s) 1352, 2504
Tru1I TTAA 6 cut(s) 552, 954, 1163, 1451, 1500, 2199
Tru9I TTAA 6 cut(s) 552, 954, 1163, 1451, 1500, 2199
TscAI CASTG 5 cut(s) 733, 1441, 1588, 1986, 2299
TseFI GTSAC 1 cut(s) 1021
Tsp45I GTSAC 1 cut(s) 1021
TspGWI ACGGA 4 cut(s) 518, 911, 2199, 2409
TspMI CCCGGG 1 cut(s) 2401
TspRI CASTG 5 cut(s) 733, 1441, 1588, 1986, 2299
Tth111I GACNNNGTC 1 cut(s) 1887
VpaK11BI GGWCC 3 cut(s) 290, 1426, 2582
XagI CCTNNNNNAGG 1 cut(s) 1656
XapI RAATTY 4 cut(s) 524, 685, 1036, 2236
XceI RCATGY 6 cut(s) 1144, 1849, 2410, 2434, 2464, 2521
XcmI CCANNNNNNNNNTGG 2 cut(s) 1606, 1634
XmaI CCCGGG 1 cut(s) 2401
XmiI GTMKAC 1 cut(s) 1891
XspI CTAG 4 cut(s) 675, 1137, 1376, 2300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.