AT3G14750

FLX-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009240
Physical Location & Seq
Reverse (-)
4953547 .. 4955754
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14750.1

Sequence Viewer

Length: 996 bp
ATGTCTGGAAGAAATCGTGGACCTCCTCCTCCATCAATGAAAGGTGGATCTTACAGTGGTTTACAAGCTCCGGTTCATCAACCGCCTTTCGTTAGAGGCTTAGGAGGAGGACCAGTGCCTCCTCCTCCTCATCCTTCTATGATTGATGATAGTAGAGAACCTCAATTTAGAGTAGATGCAAGAGGTCTTCCTCCACAATTTTCGATTCTGGAGGATCGTCTCGCTGCTCAGAATCAAGATGTTCAGGGCTTACTCGCTGATAACCAGAGACTGGCTGCGACTCATGTTGCGCTTAAACAGGAGTTAGAAGTTGCTCAGCATGAATTGCAAAGAATAATGCATTACATTGATTCCTTGAGAGCTGAGGAAGAGATTATGATGAGGGAGATGTATGATAAATCTATGCGATCCGAAATGGAATTGCGTGAAGTCGATGCTATGCGAGCTGAGATTCAAAAGATCCGTGCGGATATCAAAGAGTTTACTTCAGGTAGACAGGAACTAACGAGTCAGGTTCACCTGATGACTCAAGATTTAGCTAGACTTACAGCAGATTTGCAGCAGATACCAACGTTAACTGCAGAAATTGAGAACACAAAGCAAGAGCTGCAACGCGCAAGAGCGGCTATTGATTATGAGAAGAAAGGGTACGCGGAAAACTACGAGCATGGCAAAATTATGGAGCATAAATTAGTTGCAATGGCTCGGGAACTGGAGAAGCTTCGAGCAGAGATTGCTAACTCAGAAACGAGTGCTTATGCTAATGGTCCTGTTGGGAATCCTGGTGGAGTTGCTTATGGTGGTGGATATGGAAATCCTGAGGCTGGGTATCCTGTGAATCCTTATCAACCCAACTATACCATGAATCCAGCACAAACAGGCGTTGTTGGCTATTATCCTCCTCCTTACGGACCACAGGCTGCGTGGGCTGGTGGCTACGATCCGCAACAGCAACAGCAGCAGCAGCCGCCACCACAAGGACAAGGACATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

36.74

Weight (kDa)

5.65

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC39 PF24161 117 - 250 2.6e-06 Coiled-coil domain-containing protein 39
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 271
AccBSI CCGCTC 1 cut(s) 623
AccI GTMKAC 1 cut(s) 493
AccII CGCG 2 cut(s) 615, 653
AciI CCGC 6 cut(s) 83, 467, 623, 653, 944, 968
AclI AACGTT 1 cut(s) 572
AclWI GGATC 5 cut(s) 55, 222, 402, 454, 935
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 1 cut(s) 650
AfiI CCNNNNNNNGG 4 cut(s) 271, 824, 908, 977
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 1 cut(s) 781
AluBI AGCT 6 cut(s) 68, 362, 446, 539, 607, 721
AluI AGCT 6 cut(s) 68, 362, 446, 539, 607, 721
Alw26I GTCTC 2 cut(s) 224, 262
AlwI GGATC 5 cut(s) 55, 222, 402, 454, 935
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 705
ApeKI GCWGC 8 cut(s) 224, 275, 559, 607, 920, 958, 961, 964
AspLEI GCGC 2 cut(s) 292, 617
AspS9I GGNCC 4 cut(s) 20, 110, 767, 911
AsuHPI GGTGA 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 4 cut(s) 20, 110, 767, 911
AxyI CCTNAGG 1 cut(s) 819
BaeI ACNNNNGTAYC 2 cut(s) 557, 590
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BbsI GAAGAC 1 cut(s) 179
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 8 cut(s) 211, 262, 571, 594, 907, 970, 973, 976
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 783
BciVI GTATCC 1 cut(s) 840
BcoDI GTCTC 2 cut(s) 224, 262
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 579
BfuI GTATCC 1 cut(s) 840
BlpI GCTNAGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 4 cut(s) 20, 110, 767, 911
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 4 cut(s) 20, 110, 767, 911
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 2 cut(s) 166, 424
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 2 cut(s) 230, 734
Bpu10I CCTNAGC 2 cut(s) 100, 363
Bpu1102I GCTNAGC 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 376, 513
Bsa29I ATCGAT 1 cut(s) 991
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 824, 908, 977
Bse1I ACTGG 3 cut(s) 113, 276, 717
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 1 cut(s) 783
BseCI ATCGAT 1 cut(s) 991
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 4 cut(s) 271, 824, 908, 977
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 6 cut(s) 242, 329, 354, 438, 756, 810
BseNI ACTGG 3 cut(s) 113, 276, 717
BseRI GAGGAG 7 cut(s) 15, 18, 111, 114, 117, 120, 891
BseXI GCAGC 8 cut(s) 211, 262, 571, 594, 907, 970, 973, 976
BseYI CCCAGC 1 cut(s) 824
Bsh1236I CGCG 2 cut(s) 615, 653
BshVI ATCGAT 1 cut(s) 991
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 1 cut(s) 71
BslI CCNNNNNNNGG 4 cut(s) 271, 824, 908, 977
BsmAI GTCTC 2 cut(s) 224, 262
BsmBI CGTCTC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 705
Bsp143I GATC 5 cut(s) 47, 214, 407, 459, 940
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 6 cut(s) 83, 467, 623, 653, 944, 968
BspCNI CTCAG 6 cut(s) 241, 328, 355, 439, 755, 811
BspDI ATCGAT 1 cut(s) 991
BspFNI CGCG 2 cut(s) 615, 653
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 5 cut(s) 55, 222, 402, 454, 935
BsrBI CCGCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 3 cut(s) 113, 276, 717
BssMI GATC 5 cut(s) 47, 214, 407, 459, 940
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 363
BstAPI GCANNNNNTGC 3 cut(s) 325, 607, 734
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 7 cut(s) 100, 228, 315, 363, 447, 742, 819
BstF5I GGATG 1 cut(s) 130
BstFNI CGCG 2 cut(s) 615, 653
BstHHI GCGC 2 cut(s) 292, 617
BstKTI GATC 5 cut(s) 50, 217, 410, 462, 943
BstMAI GTCTC 2 cut(s) 224, 262
BstMBI GATC 5 cut(s) 47, 214, 407, 459, 940
BstNI CCWGG 1 cut(s) 783
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 2 cut(s) 615, 653
BstV1I GCAGC 8 cut(s) 211, 262, 571, 594, 907, 970, 973, 976
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 2 cut(s) 47, 459
BstYI RGATCY 2 cut(s) 47, 459
Bsu15I ATCGAT 1 cut(s) 991
Bsu36I CCTNAGG 1 cut(s) 819
BsuI GTATCC 1 cut(s) 840
BsuTUI ATCGAT 1 cut(s) 991
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 2 cut(s) 61, 120
Cac8I GCNNGC 1 cut(s) 444
CaiI CAGNNNCTG 1 cut(s) 271
CfoI GCGC 2 cut(s) 292, 617
Cfr13I GGNCC 4 cut(s) 20, 110, 767, 911
ClaI ATCGAT 1 cut(s) 991
Csp6I GTAC 1 cut(s) 649
CviAII CATG 4 cut(s) 284, 320, 668, 862
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 7 cut(s) 100, 228, 315, 363, 447, 742, 819
DpnI GATC 5 cut(s) 49, 216, 409, 461, 942
DpnII GATC 5 cut(s) 47, 214, 407, 459, 940
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 4 cut(s) 20, 110, 767, 911
Eco57I CTGAAG 1 cut(s) 471
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 781
EcoRV GATATC 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 342
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 4 cut(s) 287, 323, 671, 865
FatI CATG 4 cut(s) 283, 319, 667, 861
FblI GTMKAC 1 cut(s) 493
FokI GGATG 1 cut(s) 117
FspBI CTAG 1 cut(s) 540
GlaI GCGC 2 cut(s) 291, 616
GsaI CCCAGC 1 cut(s) 828
GsuI CTGGAG 2 cut(s) 230, 734
HapII CCGG 1 cut(s) 71
HhaI GCGC 2 cut(s) 292, 617
Hin1II CATG 4 cut(s) 287, 323, 671, 865
Hin6I GCGC 2 cut(s) 290, 615
HinP1I GCGC 2 cut(s) 290, 615
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HindIII AAGCTT 1 cut(s) 719
HpaI GTTAAC 1 cut(s) 576
HpaII CCGG 1 cut(s) 71
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 6 cut(s) 20, 62, 483, 494, 517, 576
Hpy188I TCNGA 3 cut(s) 231, 412, 745
Hpy188III TCNNGA 6 cut(s) 6, 209, 236, 530, 707, 818
Hpy8I GTNNAC 6 cut(s) 20, 62, 483, 494, 517, 576
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 7 cut(s) 179, 328, 340, 559, 581, 610, 698
HpyF3I CTNAG 7 cut(s) 100, 228, 315, 363, 447, 742, 819
HpySE526I ACGT 1 cut(s) 572
Hsp92II CATG 4 cut(s) 287, 323, 671, 865
HspAI GCGC 2 cut(s) 290, 615
KspAI GTTAAC 1 cut(s) 576
Kzo9I GATC 5 cut(s) 47, 214, 407, 459, 940
LmnI GCTCC 2 cut(s) 73, 682
Lsp1109I GCAGC 8 cut(s) 211, 262, 571, 594, 907, 970, 973, 976
LweI GCATC 2 cut(s) 166, 424
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 572
MalI GATC 5 cut(s) 49, 216, 409, 461, 942
MbiI CCGCTC 1 cut(s) 623
MboI GATC 5 cut(s) 47, 214, 407, 459, 940
MboII GAAGA 4 cut(s) 21, 179, 380, 652
MflI RGATCY 2 cut(s) 47, 459
MluCI AATT 7 cut(s) 164, 197, 323, 419, 585, 675, 689
MlyI GAGTC 3 cut(s) 274, 517, 520
Mph1103I ATGCAT 1 cut(s) 342
MseI TTAA 2 cut(s) 294, 575
MspI CCGG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 783
MvaI CCWGG 1 cut(s) 783
MvnI CGCG 2 cut(s) 615, 653
NdeII GATC 5 cut(s) 47, 214, 407, 459, 940
NlaIII CATG 4 cut(s) 287, 323, 671, 865
NsiI ATGCAT 1 cut(s) 342
PfeI GAWTC 7 cut(s) 205, 232, 350, 451, 778, 838, 865
PflMI CCANNNNNTGG 1 cut(s) 271
PleI GAGTC 3 cut(s) 274, 516, 520
PpsI GAGTC 3 cut(s) 274, 516, 520
Psp1406I AACGTT 1 cut(s) 572
Psp6I CCWGG 1 cut(s) 781
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 781
PspPI GGNCC 4 cut(s) 20, 110, 767, 911
PstI CTGCAG 1 cut(s) 583
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 2 cut(s) 47, 459
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
SaqAI TTAA 2 cut(s) 294, 575
Sau3AI GATC 5 cut(s) 47, 214, 407, 459, 940
Sau96I GGNCC 4 cut(s) 20, 110, 767, 911
SchI GAGTC 3 cut(s) 274, 517, 520
ScrFI CCNGG 1 cut(s) 783
SfaNI GCATC 2 cut(s) 166, 424
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 4 cut(s) 20, 110, 767, 911
SmlI CTYRAG 2 cut(s) 355, 528
SmoI CTYRAG 2 cut(s) 355, 528
Sse9I AATT 7 cut(s) 164, 197, 323, 419, 585, 675, 689
SsiI CCGC 6 cut(s) 83, 467, 623, 653, 944, 968
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 1 cut(s) 781
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 1 cut(s) 575
TaqI TCGA 4 cut(s) 203, 432, 724, 991
TasI AATT 7 cut(s) 164, 197, 323, 419, 585, 675, 689
TauI GCSGC 2 cut(s) 626, 970
TfiI GAWTC 7 cut(s) 205, 232, 350, 451, 778, 838, 865
Tru1I TTAA 2 cut(s) 294, 575
Tru9I TTAA 2 cut(s) 294, 575
TscAI CASTG 2 cut(s) 61, 120
TseI GCWGC 8 cut(s) 224, 275, 559, 607, 920, 958, 961, 964
TspDTI ATGAA 4 cut(s) 53, 65, 336, 878
TspGWI ACGGA 2 cut(s) 452, 924
TspRI CASTG 2 cut(s) 61, 120
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 4 cut(s) 20, 110, 767, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 493
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.