AT3G16250

ndh-dependent cyclic electron flow 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013153
Physical Location & Seq
Reverse (-)
5506931 .. 5508414
1484 bp
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UTR
Exon/CDS
Intron
AT3G16250.1

Sequence Viewer

Length: 615 bp
ATGGGAAGTGTACAGTTGAGTGGTTCCGGCCTAGTAGCTTCTCTACCTCCAAATCATAGCTTTAGCCACAAGACCAAACTTAATAAGCCAAATTCGTACTTCTTTCGCTCAAAACACAATGCCGCAAGAACCAAAACCGTCCGAGCCATAAGCACCGCACCAGCGAGCCAGCCTCCAGCCGCTGATGAGCCCGACGAACCTCCTGCTGTCGATTTTGCGTTCGTCCATTCGGTGTTGTTGCCGGACGGGACACCGGACGTACATTGGAGAAGAGCGAACGGTGGACAGAAACTAAGAGACATAATGTTGGATTCTAACATCGAACTCTATGGTCCTTATAGTAAGCCTTTGTCAAACTGCGCAGGAGTAGGAACTTGCGCTACTTGCATGGTCGAGATTGTAAATGGAAAGGAGCTTCTAAATCCGCGAACTGATATTGAGAAGGAGAAACTCAAAAGGAAACCAAAAAATTGGAGACTAGCTTGTCAAACCAACGTGGGAAATCCAGATTCTACCGGATTGGTTGTCATACAACAATTGCCAGAGTGGAAAGCTCATGAGTGGAACATCCCTAAGAATATACCTAATGACGATGATCTCGAAACTTCTACTTGA

Protein Analysis

204

Amino Acids

22.42

Weight (kDa)

8.33

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 119 - 166 2.9e-06 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 483
Acc16I TGCGCA 1 cut(s) 361
AccII CGCG 1 cut(s) 427
AciI CCGC 4 cut(s) 123, 156, 180, 425
AcsI RAATTY 1 cut(s) 91
AfaI GTAC 3 cut(s) 12, 98, 261
AluBI AGCT 5 cut(s) 38, 60, 415, 482, 554
AluI AGCT 5 cut(s) 38, 60, 415, 482, 554
Alw26I GTCTC 2 cut(s) 291, 469
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 1 cut(s) 28
ApoI RAATTY 1 cut(s) 91
AspLEI GCGC 2 cut(s) 362, 380
AspS9I GGNCC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BcoDI GTCTC 2 cut(s) 291, 469
BfaI CTAG 2 cut(s) 32, 479
BisI GCNGC 2 cut(s) 123, 180
BlsI GCNGC 2 cut(s) 124, 181
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 25
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 159
BsaWI WCCGGW 2 cut(s) 253, 515
BseGI GGATG 1 cut(s) 567
Bsh1236I CGCG 1 cut(s) 427
BshFI GGCC 1 cut(s) 30
BsiSI CCGG 4 cut(s) 27, 242, 254, 516
BslFI GGGAC 1 cut(s) 262
BsmAI GTCTC 2 cut(s) 291, 469
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 192
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 1 cut(s) 595
BspACI CCGC 4 cut(s) 123, 156, 180, 425
BspANI GGCC 1 cut(s) 30
BspFNI CGCG 1 cut(s) 427
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 25
BspQI GCTCTTC 1 cut(s) 265
BsrGI TGTACA 1 cut(s) 10
BssMI GATC 1 cut(s) 595
Bst4CI ACNGT 3 cut(s) 15, 139, 281
Bst6I CTCTTC 1 cut(s) 265
BstAUI TGTACA 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 166, 170
BstDEI CTNAG 2 cut(s) 293, 573
BstF5I GGATG 1 cut(s) 567
BstFNI CGCG 1 cut(s) 427
BstHHI GCGC 2 cut(s) 362, 380
BstKTI GATC 1 cut(s) 598
BstMAI GTCTC 2 cut(s) 291, 469
BstMBI GATC 1 cut(s) 595
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstUI CGCG 1 cut(s) 427
BstXI CCANNNNNNTGG 1 cut(s) 471
BsuRI GGCC 1 cut(s) 30
BtsCI GGATG 1 cut(s) 567
Cac8I GCNNGC 2 cut(s) 166, 170
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 556
CfoI GCGC 2 cut(s) 362, 380
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 3 cut(s) 11, 97, 260
CviAII CATG 2 cut(s) 388, 557
CviQI GTAC 3 cut(s) 11, 97, 260
DdeI CTNAG 2 cut(s) 293, 573
DpnI GATC 1 cut(s) 597
DpnII GATC 1 cut(s) 595
DrdI GACNNNNNNGTC 1 cut(s) 483
DseDI GACNNNNNNGTC 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 391, 560
FaiI YATR 9 cut(s) 57, 149, 302, 330, 339, 389, 530, 558, 581
FaqI GGGAC 1 cut(s) 262
FatI CATG 2 cut(s) 387, 556
Fnu4HI GCNGC 2 cut(s) 123, 180
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 123, 180
FspBI CTAG 2 cut(s) 32, 479
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 2 cut(s) 361, 379
GluI GCNGC 2 cut(s) 123, 180
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 1 cut(s) 30
HapII CCGG 4 cut(s) 27, 242, 254, 516
HhaI GCGC 2 cut(s) 362, 380
Hin1II CATG 2 cut(s) 391, 560
Hin6I GCGC 2 cut(s) 360, 378
HinP1I GCGC 2 cut(s) 360, 378
HinfI GANTC 2 cut(s) 311, 509
HpaII CCGG 4 cut(s) 27, 242, 254, 516
Hpy166II GTNNAC 2 cut(s) 11, 284
Hpy188I TCNGA 1 cut(s) 143
Hpy188III TCNNGA 4 cut(s) 394, 506, 557, 599
Hpy8I GTNNAC 2 cut(s) 11, 284
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 3 cut(s) 15, 139, 281
HpyCH4IV ACGT 2 cut(s) 258, 495
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpyF3I CTNAG 2 cut(s) 293, 573
HpySE526I ACGT 2 cut(s) 258, 495
Hsp92II CATG 2 cut(s) 391, 560
HspAI GCGC 2 cut(s) 360, 378
Kzo9I GATC 1 cut(s) 595
LguI GCTCTTC 1 cut(s) 265
LmnI GCTCC 1 cut(s) 412
MaeI CTAG 2 cut(s) 32, 479
MaeII ACGT 2 cut(s) 258, 495
MalI GATC 1 cut(s) 597
MboI GATC 1 cut(s) 595
MboII GAAGA 1 cut(s) 282
MfeI CAATTG 1 cut(s) 536
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 3 cut(s) 91, 469, 536
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 3 cut(s) 57, 183, 210
MseI TTAA 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 4 cut(s) 27, 242, 254, 516
MunI CAATTG 1 cut(s) 536
MvnI CGCG 1 cut(s) 427
MwoI GCNNNNNNNGC 1 cut(s) 384
NdeII GATC 1 cut(s) 595
NlaIII CATG 2 cut(s) 391, 560
NlaIV GGNNCC 1 cut(s) 25
NsbI TGCGCA 1 cut(s) 361
PagI TCATGA 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PfeI GAWTC 2 cut(s) 311, 509
PkrI GCNGC 2 cut(s) 124, 181
PspN4I GGNNCC 1 cut(s) 25
PspPI GGNCC 1 cut(s) 332
PsrI GAACNNNNNNTAC 2 cut(s) 364, 396
PstNI CAGNNNCTG 1 cut(s) 182
RsaI GTAC 3 cut(s) 12, 98, 261
RsaNI GTAC 3 cut(s) 11, 97, 260
SapI GCTCTTC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 2 cut(s) 123, 180
Sau3AI GATC 1 cut(s) 595
Sau96I GGNCC 1 cut(s) 332
SduI GDGCHC 1 cut(s) 192
SinI GGWCC 1 cut(s) 332
Sse9I AATT 3 cut(s) 91, 469, 536
SsiI CCGC 4 cut(s) 123, 156, 180, 425
SspMI CTAG 2 cut(s) 32, 479
TaaI ACNGT 3 cut(s) 15, 139, 281
TaiI ACGT 2 cut(s) 261, 498
TaqI TCGA 4 cut(s) 210, 321, 393, 600
TasI AATT 3 cut(s) 91, 469, 536
TatI WGTACW 1 cut(s) 10
TauI GCSGC 2 cut(s) 125, 182
TfiI GAWTC 2 cut(s) 311, 509
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 91
XspI CTAG 2 cut(s) 32, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.