MD09G1207300.v1.1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013153
Physical Location & Seq
Forward (+)
19863969 .. 19865621
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1207300.v1.1.491

Sequence Viewer

Length: 678 bp
ATGATACAAATCATCCATCCAATTCATTCAACCAGCATTAACACTCCACAACTTTTATCAATTCAAAATATATCAAAAATGGTTACTCTCCAACTGAGCTCTTGTGGTTCATCTTTTTGCTTCCTCCCATTTAACTTGATCAACTCCAACATTACCAGCTCCCATTCAACTCTCAGACCTTCCAAGCCGCTCATCAGTTTCTCCAGAGCAAAAATTAGAGCAATTAGCACATCCCAAGAGAGCCAATCCGAAGCCGTTCAACCCCAGCAATCCCCTTCCGTGGATTTCGCATTCGTTAATTCGGTTTTACTTCCTGACCAGACACCAGATGTGCATCTTCGTCAAGCCTGTGGAGGGCAGAAACTTAGGAATATAATGCTGGATTCAAATATCGATTTGTATGGACCATACGGTAGAGTTCTGTTGAATTGTGCTGGAGGAGGAACCTGCGGGACATGTATGGTGGAGATTGTTCAAGGAAAAGAGCTGTTAACCCCTCGCACAGACAAAGAAAAGGAACATCTTAAAAAGAAACCAAAAAATTGGAGACTTGCTTGCCAAACCACAGTTGGGAAACCTGACTCCAGAGGGCTGGTTGTTGTCCAACAGCTGCCAGAATGGAAAGCACATGAATGGAAATACGAAGAAGTCCTAGTAGACCATTCACAGTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

226

Amino Acids

24.95

Weight (kDa)

8.85

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 142 - 190 2.2e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 657
AciI CCGC 2 cut(s) 188, 450
AfiI CCNNNNNNNGG 3 cut(s) 280, 354, 570
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 7 cut(s) 30, 65, 168, 260, 387, 427, 476
AluBI AGCT 4 cut(s) 99, 159, 487, 610
AluI AGCT 4 cut(s) 99, 159, 487, 610
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 1 cut(s) 541
ApeKI GCWGC 1 cut(s) 610
Asp700I GAANNNNTTC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 404
AvaII GGWCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 597
BccI CCATC 1 cut(s) 24
BceAI ACGGC 1 cut(s) 239
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 541
BfaI CTAG 1 cut(s) 653
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 2 cut(s) 188, 611
BlsI GCNGC 2 cut(s) 189, 612
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 445
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 3 cut(s) 187, 456, 568
Bsa29I ATCGAT 1 cut(s) 393
BsaBI GATNNNNATC 2 cut(s) 8, 333
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 354, 570
Bse8I GATNNNNATC 2 cut(s) 8, 333
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 3 cut(s) 12, 16, 230
BseJI GATNNNNATC 2 cut(s) 8, 333
BseLI CCNNNNNNNGG 3 cut(s) 280, 354, 570
BseMII CTCAG 2 cut(s) 86, 187
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 597
BseYI CCCAGC 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 393
BsiHKAI GWGCWC 1 cut(s) 101
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 3 cut(s) 280, 354, 570
BsmAI GTCTC 1 cut(s) 541
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 2 cut(s) 188, 450
BspCNI CTCAG 2 cut(s) 87, 186
BspDI ATCGAT 1 cut(s) 393
BspLI GGNNCC 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 413, 568, 669
BstC8I GCNNGC 1 cut(s) 556
BstDEI CTNAG 3 cut(s) 95, 173, 365
BstDSI CCRYGG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 12, 16, 230
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 1 cut(s) 541
BstMBI GATC 1 cut(s) 138
BstNSI RCATGY 1 cut(s) 459
BstV1I GCAGC 1 cut(s) 597
BstV2I GAAGAC 1 cut(s) 663
BstXI CCANNNNNNTGG 2 cut(s) 543, 592
Bsu15I ATCGAT 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 3 cut(s) 12, 16, 230
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 556
Cfr13I GGNCC 1 cut(s) 404
ClaI ATCGAT 1 cut(s) 393
CviAII CATG 2 cut(s) 456, 629
CviJI RGCY 9 cut(s) 99, 159, 187, 243, 254, 347, 487, 592, 610
CviKI_1 RGCY 9 cut(s) 99, 159, 187, 243, 254, 347, 487, 592, 610
DdeI CTNAG 3 cut(s) 95, 173, 365
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 101
FaeI CATG 2 cut(s) 459, 632
FaiI YATR 8 cut(s) 71, 374, 402, 409, 457, 461, 630, 676
FaqI GGGAC 1 cut(s) 466
FatI CATG 2 cut(s) 455, 628
FauI CCCGC 1 cut(s) 443
FbaI TGATCA 1 cut(s) 138
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 188, 611
FokI GGATG 2 cut(s) 3, 217
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 188, 611
FspBI CTAG 1 cut(s) 653
GluI GCNGC 2 cut(s) 188, 611
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 3 cut(s) 187, 456, 568
Hin1II CATG 2 cut(s) 459, 632
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 2 cut(s) 383, 581
HpaI GTTAAC 1 cut(s) 492
Hpy166II GTNNAC 2 cut(s) 492, 658
Hpy188I TCNGA 2 cut(s) 176, 250
Hpy188III TCNNGA 3 cut(s) 204, 314, 585
Hpy8I GTNNAC 2 cut(s) 492, 658
HpyAV CCTTC 2 cut(s) 189, 285
HpyCH4III ACNGT 3 cut(s) 413, 568, 669
HpyCH4V TGCA 1 cut(s) 334
HpyF3I CTNAG 3 cut(s) 95, 173, 365
Hsp92II CATG 2 cut(s) 459, 632
Ksp22I TGATCA 1 cut(s) 138
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 164
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 653
MaeIII GTNAC 1 cut(s) 82
MalI GATC 1 cut(s) 140
MbiI CCGCTC 1 cut(s) 190
MboI GATC 1 cut(s) 138
MboII GAAGA 3 cut(s) 329, 656, 663
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 7 cut(s) 21, 60, 213, 222, 298, 427, 541
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 3 cut(s) 115, 171, 628
MnlI CCTC 6 cut(s) 134, 347, 431, 434, 507, 581
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 5 cut(s) 39, 132, 297, 491, 525
MslI CAYNNNNRTG 1 cut(s) 631
MspA1I CMGCKG 1 cut(s) 610
Mva1269I GAATGC 1 cut(s) 290
NdeII GATC 1 cut(s) 138
NlaIII CATG 2 cut(s) 459, 632
NlaIV GGNNCC 1 cut(s) 445
NspI RCATGY 1 cut(s) 459
PciI ACATGT 1 cut(s) 455
PctI GAATGC 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 2 cut(s) 189, 612
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PscI ACATGT 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 101
PspFI CCCAGC 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 445
PspPI GGNCC 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 610
RseI CAYNNNNRTG 1 cut(s) 631
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 5 cut(s) 39, 132, 297, 491, 525
SatI GCNGC 2 cut(s) 188, 611
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 1 cut(s) 575
SduI GDGCHC 1 cut(s) 101
SetI ASST 7 cut(s) 101, 161, 181, 449, 489, 580, 612
SfaNI GCATC 1 cut(s) 343
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 631
Sse9I AATT 7 cut(s) 21, 60, 213, 222, 298, 427, 541
SsiI CCGC 2 cut(s) 188, 450
SspMI CTAG 1 cut(s) 653
SstI GAGCTC 1 cut(s) 101
TaaI ACNGT 3 cut(s) 413, 568, 669
TaqI TCGA 1 cut(s) 393
TasI AATT 7 cut(s) 21, 60, 213, 222, 298, 427, 541
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 5 cut(s) 39, 132, 297, 491, 525
Tru9I TTAA 5 cut(s) 39, 132, 297, 491, 525
TseI GCWGC 1 cut(s) 610
TspDTI ATGAA 4 cut(s) 14, 99, 645, 663
TspGWI ACGGA 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 404
XceI RCATGY 1 cut(s) 459
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 657
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.