FvH4_6g31980

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013153
Physical Location & Seq
Reverse (-)
25076199 .. 25077865
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31980.t2

Sequence Viewer

Length: 600 bp
ATGGGTACTCTCCAGTTGAGCTCTTGTGGTTCATCATTTTGCTCACCCATCCCTTCCAACCTGATTAGTCACAACATTACCACCTCCCATTCAACTCCCAGCTCACTCACTTTCTCCAGAGGGAAAATCAGAGCTGTTACCACAGTCCCAAATAGTAGTGAACCTGCACAAGCCATTGAACCAGAACAGGAACCCCCTCTGGTTGATTTTGCATTTGTTAGCTCTGTGTTGCTACCTGATCAAACTCCGGATGTGCATCTTCGTCAAGCATGTGGAGGACAGAAGCTTAGGAATATAATGTTGGATGGAAATATTGAATTGTATGGACCATATGCTAGACCTCTGCTGAATTGTGCTGGAGGAGGAACCTGTGGAACATGTATGGTGGAGGTTATTAAAGGAAAGGAGCTCTTAACACCTAGGACAGACAAAGAAAAGGAACACCTTCAAAAGAAACCAAAGAACTGGAGACTTGCTTGCCAAACCACAGTAGGGAAACCTGACTCCAGAGGGCTGGTTGTTGTTCAACAACTACCTGAATGGAAAGCACATGAATGGAAGTATGAGAATGTCCTAGTGAACCCTTCACAGTCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

200

Amino Acids

21.72

Weight (kDa)

8.16

Isoelectric Point (pI)

56.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 117 - 164 1.9e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AccIII TCCGGA 1 cut(s) 247
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 492
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 5 cut(s) 93, 179, 317, 449, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 6 cut(s) 21, 102, 134, 222, 286, 409
AluI AGCT 6 cut(s) 21, 102, 134, 222, 286, 409
Alw21I GWGCWC 2 cut(s) 23, 411
Alw26I GTCTC 1 cut(s) 463
Aor13HI TCCGGA 1 cut(s) 247
Asp700I GAANNNNTTC 1 cut(s) 444
AspA2I CCTAGG 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 36
AvaII GGWCC 1 cut(s) 326
AvrII CCTAGG 1 cut(s) 419
BanII GRGCYC 2 cut(s) 23, 411
Bbv12I GWGCWC 2 cut(s) 23, 411
BccI CCATC 2 cut(s) 56, 299
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 3 cut(s) 336, 420, 575
BfuAI ACCTGC 1 cut(s) 172
BlnI CCTAGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 192, 367
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 4 cut(s) 100, 378, 487, 490
Bpu10I CCTNAGC 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse1I ACTGG 2 cut(s) 13, 470
Bse8I GATNNNNATC 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 3 cut(s) 48, 256, 310
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 492
BseNI ACTGG 2 cut(s) 13, 470
BseRI GAGGAG 1 cut(s) 375
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 23, 411
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 23, 411
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 1 cut(s) 238
BspEI TCCGGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 192, 367
BspMI ACCTGC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 13, 470
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 419
Bst4CI ACNGT 3 cut(s) 145, 490, 591
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 1 cut(s) 287
BstF5I GGATG 3 cut(s) 48, 256, 310
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 1 cut(s) 238
BstNSI RCATGY 2 cut(s) 273, 381
BstXI CCANNNNNNTGG 2 cut(s) 465, 514
BtsCI GGATG 3 cut(s) 48, 256, 310
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 6
CviAII CATG 3 cut(s) 270, 378, 551
CviJI RGCY 8 cut(s) 21, 102, 134, 173, 222, 286, 409, 514
CviKI_1 RGCY 8 cut(s) 21, 102, 134, 173, 222, 286, 409, 514
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 287
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Ecl136II GAGCTC 2 cut(s) 21, 409
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 2 cut(s) 23, 411
Eco47I GGWCC 1 cut(s) 326
Eco53kI GAGCTC 2 cut(s) 21, 409
EcoICRI GAGCTC 2 cut(s) 21, 409
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 2 cut(s) 23, 411
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 3 cut(s) 273, 381, 554
FaiI YATR 9 cut(s) 271, 296, 324, 331, 333, 379, 383, 552, 564
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 131
FatI CATG 3 cut(s) 269, 377, 550
FauNDI CATATG 1 cut(s) 331
FbaI TGATCA 1 cut(s) 238
FokI GGATG 3 cut(s) 35, 263, 317
FriOI GRGCYC 2 cut(s) 23, 411
FspBI CTAG 3 cut(s) 336, 420, 575
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 4 cut(s) 100, 378, 487, 490
HapII CCGG 1 cut(s) 248
Hin1II CATG 3 cut(s) 273, 381, 554
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 1 cut(s) 503
HpaII CCGG 1 cut(s) 248
HphI GGTGA 1 cut(s) 36
Hpy166II GTNNAC 2 cut(s) 161, 580
Hpy188I TCNGA 1 cut(s) 131
Hpy188III TCNNGA 3 cut(s) 117, 248, 507
Hpy8I GTNNAC 2 cut(s) 161, 580
HpyAV CCTTC 3 cut(s) 63, 455, 594
HpyCH4III ACNGT 3 cut(s) 145, 490, 591
HpyCH4V TGCA 3 cut(s) 167, 212, 256
HpyF3I CTNAG 1 cut(s) 287
Hsp92II CATG 3 cut(s) 273, 381, 554
Kpn2I TCCGGA 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 1 cut(s) 406
LweI GCATC 1 cut(s) 265
MaeI CTAG 3 cut(s) 336, 420, 575
MaeIII GTNAC 2 cut(s) 68, 136
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 23, 411
MluCI AATT 2 cut(s) 317, 349
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 2 cut(s) 81, 282
MnlI CCTC 9 cut(s) 94, 113, 207, 269, 351, 353, 356, 382, 503
MroI TCCGGA 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 3 cut(s) 396, 413, 598
MslI CAYNNNNRTG 1 cut(s) 553
MspI CCGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 331
NdeII GATC 1 cut(s) 238
NlaIII CATG 3 cut(s) 273, 381, 554
NlaIV GGNNCC 2 cut(s) 192, 367
NmuCI GTSAC 1 cut(s) 68
NspI RCATGY 2 cut(s) 273, 381
PciI ACATGT 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 444
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 377
Psp124BI GAGCTC 2 cut(s) 23, 411
PspFI CCCAGC 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 192, 367
PspPI GGNCC 1 cut(s) 326
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 553
SacI GAGCTC 2 cut(s) 23, 411
SaqAI TTAA 3 cut(s) 396, 413, 598
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 2 cut(s) 23, 411
SfaNI GCATC 1 cut(s) 265
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 553
Sse9I AATT 2 cut(s) 317, 349
SspI AATATT 1 cut(s) 313
SspMI CTAG 3 cut(s) 336, 420, 575
SstI GAGCTC 2 cut(s) 23, 411
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 3 cut(s) 145, 490, 591
TasI AATT 2 cut(s) 317, 349
Tru1I TTAA 3 cut(s) 396, 413, 598
Tru9I TTAA 3 cut(s) 396, 413, 598
TseFI GTSAC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 2 cut(s) 21, 567
VpaK11BI GGWCC 1 cut(s) 326
XceI RCATGY 2 cut(s) 273, 381
XmaJI CCTAGG 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 336, 420, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.