MD17G1188300.v1.1

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013153
Physical Location & Seq
Forward (+)
22459365 .. 22460847
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1188300.v1.1.491

Sequence Viewer

Length: 669 bp
ATGCTGCAAACAAACCAAAACCCCATGCAAATCATCCGTCCAGTTCATTTCAGTTCAAATATATCAAAAATGGTTGCTCTCCAACTGAGCTCTTGCTGGTCATCTTTTTGCTCCCTCCCATTTAACTTGATCAACTCCAACATTACCAACTCCCATTCAACTCTCAGACCTTCCAAGCCCCTCATCAGTTTCTCCAGAGCAAAAATTAGAGCAGTTAGCACATCCCAAGAGAGCCAATCTGAAGCCATTCAACCCGAGCAACCCCCTTCCGTGGATTTCGCATTTGTTAGTTCAGTTTTACTTCCTGACCAAACACCAGATGTGCATCTTCGTCAAGCCTGTGGAGGACAGAAACTTAGGAATATAATGTTGGATTCAAATATCGATTTGTACGGACCATACAGTAGAGTTCTGCTGAATTGTGCTGGAGGAGGAACCTGCGGGACATGCATGGTGGAGATTGTTCAAGGAAAAGAGCTGTTAACCCCTCGCACAGACAAAGAGAAGGAACACCTTAAAAAGAAACCAAAAAATTGGAGACTTGCTTGCCAAACCACAGTTGGGAAACCTGACTCTAGAGGGCTGGTTGTTGTCCAACAGCTGCCAGAATGGAAAGCCCATGAATGGAAATATGAAGAAGTCCTGGTAGACCCTAACCAGTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

223

Amino Acids

24.78

Weight (kDa)

8.85

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 139 - 187 2.1e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 648
AciI CCGC 1 cut(s) 441
AcuI CTGAAG 1 cut(s) 261
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 3 cut(s) 271, 561, 624
AgsI TTSAA 5 cut(s) 57, 159, 251, 378, 467
AjnI CCWGG 1 cut(s) 642
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 3 cut(s) 90, 478, 601
AluI AGCT 3 cut(s) 90, 478, 601
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 4, 601
ArsI GACNNNNNNTTYG 2 cut(s) 22, 54
Asp700I GAANNNNTTC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 588
BciT130I CCWGG 1 cut(s) 644
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 446
BisI GCNGC 2 cut(s) 5, 602
BlsI GCNGC 2 cut(s) 6, 603
Bme1390I CCNGG 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 1 cut(s) 436
BmrFI CCNGG 1 cut(s) 644
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 654
BpmI CTGGAG 2 cut(s) 178, 447
Bsa29I ATCGAT 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 270
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 561, 624
Bse1I ACTGG 2 cut(s) 41, 658
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 644
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 33, 221
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 271, 561, 624
BseMII CTCAG 2 cut(s) 77, 178
BseNI ACTGG 2 cut(s) 41, 658
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 3 cut(s) 271, 561, 624
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 1 cut(s) 441
BspCNI CTCAG 2 cut(s) 78, 177
BspDI ATCGAT 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 446
BsrI ACTGG 2 cut(s) 41, 658
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 2 cut(s) 404, 559
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 3 cut(s) 86, 164, 356
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 33, 221
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 447
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 642
BstV1I GCAGC 1 cut(s) 588
BstV2I GAAGAC 1 cut(s) 654
BstXI CCANNNNNNTGG 1 cut(s) 534
Bsu15I ATCGAT 1 cut(s) 384
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 33, 221
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 384
Csp6I GTAC 1 cut(s) 391
CviAII CATG 4 cut(s) 25, 447, 451, 620
CviJI RGCY 9 cut(s) 90, 178, 234, 245, 338, 478, 583, 601, 617
CviKI_1 RGCY 9 cut(s) 90, 178, 234, 245, 338, 478, 583, 601, 617
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 86, 164, 356
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 395
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 642
EcoT22I ATGCAT 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 92
FaeI CATG 4 cut(s) 28, 450, 454, 623
FaiI YATR 9 cut(s) 26, 62, 365, 400, 448, 452, 621, 633, 667
FaqI GGGAC 1 cut(s) 457
FatI CATG 4 cut(s) 24, 446, 450, 619
FauI CCCGC 1 cut(s) 434
FbaI TGATCA 1 cut(s) 129
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 2 cut(s) 5, 602
FokI GGATG 2 cut(s) 20, 208
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 2 cut(s) 5, 602
FspBI CTAG 1 cut(s) 576
GluI GCNGC 2 cut(s) 5, 602
GsuI CTGGAG 2 cut(s) 178, 447
Hin1II CATG 4 cut(s) 28, 450, 454, 623
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HinfI GANTC 2 cut(s) 374, 572
HpaI GTTAAC 1 cut(s) 483
Hpy166II GTNNAC 2 cut(s) 483, 649
Hpy188I TCNGA 2 cut(s) 167, 241
Hpy188III TCNNGA 3 cut(s) 195, 305, 576
Hpy8I GTNNAC 2 cut(s) 483, 649
HpyAV CCTTC 3 cut(s) 180, 276, 499
HpyCH4III ACNGT 2 cut(s) 404, 559
HpyCH4V TGCA 4 cut(s) 7, 28, 325, 450
HpyF10VI GCNNNNNNNGC 1 cut(s) 447
HpyF3I CTNAG 3 cut(s) 86, 164, 356
Hsp92II CATG 4 cut(s) 28, 450, 454, 623
Ksp22I TGATCA 1 cut(s) 129
KspAI GTTAAC 1 cut(s) 483
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 1 cut(s) 588
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 576
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 3 cut(s) 320, 647, 654
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 3 cut(s) 204, 418, 532
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 4 cut(s) 106, 162, 351, 619
MnlI CCTC 7 cut(s) 125, 191, 338, 422, 425, 498, 572
Mph1103I ATGCAT 1 cut(s) 452
MroXI GAANNNNTTC 1 cut(s) 246
MseI TTAA 3 cut(s) 123, 482, 516
MspA1I CMGCKG 1 cut(s) 601
MspR9I CCNGG 1 cut(s) 644
MvaI CCWGG 1 cut(s) 644
MwoI GCNNNNNNNGC 1 cut(s) 447
NdeII GATC 1 cut(s) 129
NlaIII CATG 4 cut(s) 28, 450, 454, 623
NlaIV GGNNCC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 452
NspI RCATGY 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 246
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 2 cut(s) 6, 603
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 642
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 1 cut(s) 436
PspPI GGNCC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SacI GAGCTC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 123, 482, 516
SatI GCNGC 2 cut(s) 5, 602
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 1 cut(s) 395
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 1 cut(s) 644
SduI GDGCHC 1 cut(s) 92
SetI ASST 7 cut(s) 92, 172, 440, 480, 516, 571, 603
SfaNI GCATC 1 cut(s) 334
SinI GGWCC 1 cut(s) 395
Sse9I AATT 3 cut(s) 204, 418, 532
SsiI CCGC 1 cut(s) 441
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 642
TaaI ACNGT 2 cut(s) 404, 559
TaqI TCGA 1 cut(s) 384
TasI AATT 3 cut(s) 204, 418, 532
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 3 cut(s) 123, 482, 516
Tru9I TTAA 3 cut(s) 123, 482, 516
TseI GCWGC 2 cut(s) 4, 601
TspDTI ATGAA 4 cut(s) 35, 636, 648, 654
TspGWI ACGGA 3 cut(s) 26, 259, 408
VpaK11BI GGWCC 1 cut(s) 395
XbaI TCTAGA 1 cut(s) 575
XceI RCATGY 1 cut(s) 450
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 1 cut(s) 246
XspI CTAG 1 cut(s) 576
Zsp2I ATGCAT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.