Rroxscaffold_2G00105390

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013153
Physical Location & Seq
Forward (+)
28810598 .. 28812139
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00105390.1

Sequence Viewer

Length: 594 bp
ATGGGTACTCTCCAGTTGAGCTCTTGTGGTTCATCATTTTGCTCACCCCTCCCTTCTAACCTGATTAGTCACAACATTACCACCTCCCATTCAACTCCCAAGTCCCTCACTTTCTCCAGAGGAAAAATAAGAGCAGTTAGCACAGCTCCAAATAGTAGTGAATCTGCACAAGCCATTGAACCAGAACAACAACCTCCTCTGGTTGATTTTGCATTTGTTAACTCTGTGTTGCTACCCGATCAAACCCCGGATGTGCATCTTCGTCAAGCCTGTGGAGGACAGAAGCTTAGGAATATAATGTTGGATGGAAATATTGAATTGTATGGACCATATGCTAGACCTCTGCTGAATTGTGGTGGTGGAGGAACCTGTGGAACATGTATGGTGGAGGTTATTAAAGGAAAGGAGCTCTTAAGCCCTCGGACAGACAAAGAAAAGAAACACCTTCAAAAGAAACCAAAGAATTGGAGACTTGCTTGCCAAGCCACAGTAGGGAAACCTGACTCCAGAGGGCTGGTTGTTGTTCAACAACTACCTGAATGGAAAGCACATGAATGGAAGTATGAGGACACCCTAGTGAACCCTTCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

197

Amino Acids

21.51

Weight (kDa)

8.85

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 117 - 164 6.2e-09 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 492
AflII CTTAAG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 5 cut(s) 93, 179, 317, 449, 527
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AleI CACNNNNGTG 1 cut(s) 575
AluBI AGCT 4 cut(s) 21, 146, 286, 409
AluI AGCT 4 cut(s) 21, 146, 286, 409
Alw21I GWGCWC 2 cut(s) 23, 411
Alw26I GTCTC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 36
AvaII GGWCC 1 cut(s) 326
BanII GRGCYC 2 cut(s) 23, 411
Bbv12I GWGCWC 2 cut(s) 23, 411
BccI CCATC 1 cut(s) 299
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 2 cut(s) 336, 575
BfrI CTTAAG 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 2 cut(s) 100, 490
Bpu10I CCTNAGC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 246, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse1I ACTGG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 246, 419
BseGI GGATG 2 cut(s) 256, 310
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 492
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 186
BsgI GTGCAG 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 23, 411
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 23, 411
Bsp143I GATC 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 367
BspTI CTTAAG 1 cut(s) 412
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 246, 419
BssMI GATC 1 cut(s) 238
Bst4CI ACNGT 2 cut(s) 490, 591
BstAFI CTTAAG 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 1 cut(s) 287
BstF5I GGATG 2 cut(s) 256, 310
BstKTI GATC 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 246
BstXI CCANNNNNNTGG 2 cut(s) 465, 514
BtsCI GGATG 2 cut(s) 256, 310
Cac8I GCNNGC 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 378, 551
CviJI RGCY 9 cut(s) 21, 146, 173, 269, 286, 409, 417, 485, 514
CviKI_1 RGCY 9 cut(s) 21, 146, 173, 269, 286, 409, 417, 485, 514
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 287
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
Ecl136II GAGCTC 2 cut(s) 21, 409
Eco24I GRGCYC 2 cut(s) 23, 411
Eco47I GGWCC 1 cut(s) 326
Eco53kI GAGCTC 2 cut(s) 21, 409
EcoICRI GAGCTC 2 cut(s) 21, 409
EcoT38I GRGCYC 2 cut(s) 23, 411
FaeI CATG 2 cut(s) 381, 554
FaiI YATR 8 cut(s) 296, 324, 331, 333, 379, 383, 552, 564
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 88
FatI CATG 2 cut(s) 377, 550
FauNDI CATATG 1 cut(s) 331
FokI GGATG 2 cut(s) 263, 317
FriOI GRGCYC 2 cut(s) 23, 411
FspBI CTAG 2 cut(s) 336, 575
GsuI CTGGAG 2 cut(s) 100, 490
HapII CCGG 1 cut(s) 248
Hin1II CATG 2 cut(s) 381, 554
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 2 cut(s) 161, 503
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 1 cut(s) 248
HphI GGTGA 1 cut(s) 36
Hpy166II GTNNAC 2 cut(s) 220, 580
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 2 cut(s) 117, 507
Hpy8I GTNNAC 2 cut(s) 220, 580
HpyAV CCTTC 3 cut(s) 63, 455, 594
HpyCH4III ACNGT 2 cut(s) 490, 591
HpyCH4V TGCA 3 cut(s) 167, 212, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 1 cut(s) 287
Hsp92II CATG 2 cut(s) 381, 554
KspAI GTTAAC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 238
LmnI GCTCC 2 cut(s) 151, 406
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 336, 575
MaeIII GTNAC 1 cut(s) 68
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 23, 411
MluCI AATT 3 cut(s) 317, 349, 463
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 1 cut(s) 282
MseI TTAA 3 cut(s) 219, 396, 413
MslI CAYNNNNRTG 2 cut(s) 553, 575
MspCI CTTAAG 1 cut(s) 412
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 482
NciI CCSGG 1 cut(s) 248
NdeI CATATG 1 cut(s) 331
NdeII GATC 1 cut(s) 238
NlaIII CATG 2 cut(s) 381, 554
NlaIV GGNNCC 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 68
NspI RCATGY 1 cut(s) 381
OliI CACNNNNGTG 1 cut(s) 575
PciI ACATGT 1 cut(s) 377
PfeI GAWTC 1 cut(s) 161
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 377
Psp124BI GAGCTC 2 cut(s) 23, 411
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 326
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 2 cut(s) 553, 575
SacI GAGCTC 2 cut(s) 23, 411
SaqAI TTAA 3 cut(s) 219, 396, 413
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 2 cut(s) 23, 411
SfaNI GCATC 1 cut(s) 265
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 553, 575
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 3 cut(s) 317, 349, 463
SspI AATATT 1 cut(s) 313
SspMI CTAG 2 cut(s) 336, 575
SstI GAGCTC 2 cut(s) 23, 411
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 490, 591
TasI AATT 3 cut(s) 317, 349, 463
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 3 cut(s) 219, 396, 413
Tru9I TTAA 3 cut(s) 219, 396, 413
TseFI GTSAC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 2 cut(s) 21, 567
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 326
XceI RCATGY 1 cut(s) 381
XspI CTAG 2 cut(s) 336, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.