AT3G17130

cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014918
Physical Location & Seq
Reverse (-)
5844277 .. 5845220
944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G17130.1

Sequence Viewer

Length: 552 bp
ATGAAAAGATCATCCACCGTCACATCATTACCGTGTCTCTCCCTCTTTCTCCTTCTGCTTCTGCCGTCGGTAGTCCAATCCTCCGACGACCTTATCGACAAAATATGCCAAGCAACGCCATTCTGCGACCTATGTGAAGCCTCTCTTCGTCCATTCTCTCCGTCACCTTCTGATCCCAAATCCTTAGGTGCCGCTATGGCCAGCGTCGTGCTCGGAAACATGACCGATACTTTAGGATACATCCAATCGCTGATCAAACATGCTCACGATCCTGCGGCAGAGCGCGCGCTGGCTCAGTGCGCTGAGCTGTACCGGCCGGTGGTCAAGTTTAATATTCCTCAGGCGATGGAGGCAATGCAGGGCGGCAAATTCGGCTTTGCGATATATGTGCTCGGAGACGCCGAGAAGCAGACTGATTCTTGTCAGAAAGGAATCACGAACGCCGGCGCCGACGATGAAAGCTCGGTGGCTGTTACGGCTAGAAACAAGCTAGTTAAAAATCTTTGTGACGTGGCGATTTCTGTGCTTAAGTCTCTGATGAATGGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

183

Amino Acids

19.45

Weight (kDa)

5.64

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 174 6.6e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 446
AccII CGCG 2 cut(s) 285, 287
AciI CCGC 3 cut(s) 192, 275, 363
AclWI GGATC 2 cut(s) 167, 263
AcoI YGGCCR 2 cut(s) 198, 314
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 2 cut(s) 399, 447
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 319
AflII CTTAAG 1 cut(s) 527
AjiI CACGTC 1 cut(s) 511
AluBI AGCT 3 cut(s) 307, 462, 490
AluI AGCT 3 cut(s) 307, 462, 490
Alw21I GWGCWC 2 cut(s) 213, 393
Alw26I GTCTC 3 cut(s) 41, 390, 537
AlwI GGATC 2 cut(s) 167, 263
AoxI GGCC 2 cut(s) 198, 314
ApoI RAATTY 1 cut(s) 368
AspLEI GCGC 5 cut(s) 285, 287, 289, 302, 449
AsuHPI GGTGA 1 cut(s) 156
AxyI CCTNAGG 2 cut(s) 184, 339
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 2 cut(s) 188, 446
Bbv12I GWGCWC 2 cut(s) 213, 393
BccI CCATC 1 cut(s) 340
BceAI ACGGC 2 cut(s) 49, 492
BcgI CGANNNNNNTGC 4 cut(s) 370, 404, 505, 539
BciVI GTATCC 1 cut(s) 230
BclI TGATCA 1 cut(s) 252
BcoDI GTCTC 3 cut(s) 41, 390, 537
BfaI CTAG 2 cut(s) 480, 491
BfoI RGCGCY 1 cut(s) 450
BfrI CTTAAG 1 cut(s) 527
BfuI GTATCC 1 cut(s) 230
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 3 cut(s) 192, 276, 364
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 3 cut(s) 193, 277, 365
BmgBI CACGTC 1 cut(s) 511
BmiI GGNNCC 2 cut(s) 190, 448
Bpu1102I GCTNAGC 1 cut(s) 303
BsaHI GRCGYC 2 cut(s) 399, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse118I RCCGGY 3 cut(s) 312, 316, 443
Bse21I CCTNAGG 2 cut(s) 184, 339
Bse3DI GCAATG 1 cut(s) 360
BseGI GGATG 2 cut(s) 11, 240
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 3 cut(s) 294, 308, 353
BsePI GCGCGC 2 cut(s) 283, 285
BseX3I CGGCCG 1 cut(s) 314
Bsh1236I CGCG 2 cut(s) 285, 287
Bsh1285I CGRYCG 1 cut(s) 317
BshFI GGCC 2 cut(s) 200, 316
BshNI GGYRCC 2 cut(s) 188, 446
BsiEI CGRYCG 1 cut(s) 317
BsiHKAI GWGCWC 2 cut(s) 213, 393
BsiSI CCGG 3 cut(s) 313, 317, 444
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 3 cut(s) 41, 390, 537
BsmBI CGTCTC 1 cut(s) 390
BsnI GGCC 2 cut(s) 200, 316
Bsp1286I GDGCHC 2 cut(s) 213, 393
Bsp143I GATC 4 cut(s) 8, 172, 252, 268
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 3 cut(s) 192, 275, 363
BspANI GGCC 2 cut(s) 200, 316
BspCNI CTCAG 3 cut(s) 295, 307, 352
BspFNI CGCG 2 cut(s) 285, 287
BspLI GGNNCC 2 cut(s) 190, 448
BspPI GGATC 2 cut(s) 167, 263
BspT107I GGYRCC 2 cut(s) 188, 446
BspTI CTTAAG 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 3 cut(s) 312, 316, 443
BssAI RCCGGY 3 cut(s) 312, 316, 443
BssHII GCGCGC 2 cut(s) 283, 285
BssMI GATC 4 cut(s) 8, 172, 252, 268
BssNI GRCGYC 2 cut(s) 399, 447
Bst4CI ACNGT 2 cut(s) 19, 33
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 2 cut(s) 399, 447
BstAFI CTTAAG 1 cut(s) 527
BstC8I GCNNGC 5 cut(s) 202, 285, 287, 291, 445
BstDEI CTNAG 4 cut(s) 184, 294, 303, 339
BstF5I GGATG 2 cut(s) 11, 240
BstFNI CGCG 2 cut(s) 285, 287
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 5 cut(s) 285, 287, 289, 302, 449
BstKTI GATC 4 cut(s) 11, 175, 255, 271
BstMAI GTCTC 3 cut(s) 41, 390, 537
BstMBI GATC 4 cut(s) 8, 172, 252, 268
BstMCI CGRYCG 1 cut(s) 317
BstMWI GCNNNNNNNGC 7 cut(s) 197, 284, 299, 313, 350, 372, 476
BstNSI RCATGY 1 cut(s) 263
BstUI CGCG 2 cut(s) 285, 287
BstZI CGGCCG 1 cut(s) 314
Bsu36I CCTNAGG 2 cut(s) 184, 339
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 2 cut(s) 200, 316
BtgZI GCGATG 1 cut(s) 359
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 2 cut(s) 11, 240
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 5 cut(s) 202, 285, 287, 291, 445
CfoI GCGC 5 cut(s) 285, 287, 289, 302, 449
Cfr10I RCCGGY 3 cut(s) 312, 316, 443
CseI GACGC 2 cut(s) 193, 407
Csp6I GTAC 1 cut(s) 310
CviAII CATG 2 cut(s) 220, 260
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 4 cut(s) 184, 294, 303, 339
DinI GGCGCC 1 cut(s) 448
DpnI GATC 4 cut(s) 10, 174, 254, 270
DpnII GATC 4 cut(s) 8, 172, 252, 268
EaeI YGGCCR 2 cut(s) 198, 314
EagI CGGCCG 1 cut(s) 314
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EclXI CGGCCG 1 cut(s) 314
Eco52I CGGCCG 1 cut(s) 314
Eco81I CCTNAGG 2 cut(s) 184, 339
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 2 cut(s) 223, 263
FaiI YATR 7 cut(s) 106, 133, 197, 221, 261, 385, 387
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 2 cut(s) 219, 259
FbaI TGATCA 1 cut(s) 252
Fnu4HI GCNGC 3 cut(s) 192, 276, 364
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 192, 276, 364
FspBI CTAG 2 cut(s) 480, 491
GlaI GCGC 5 cut(s) 284, 286, 288, 301, 448
GluI GCNGC 3 cut(s) 192, 276, 364
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 2 cut(s) 200, 316
HapII CCGG 3 cut(s) 313, 317, 444
HgaI GACGC 2 cut(s) 193, 407
HhaI GCGC 5 cut(s) 285, 287, 289, 302, 449
Hin1I GRCGYC 2 cut(s) 399, 447
Hin1II CATG 2 cut(s) 223, 263
Hin6I GCGC 5 cut(s) 283, 285, 287, 300, 447
HinP1I GCGC 5 cut(s) 283, 285, 287, 300, 447
HinfI GANTC 2 cut(s) 416, 432
HpaII CCGG 3 cut(s) 313, 317, 444
HphI GGTGA 1 cut(s) 156
Hpy188I TCNGA 6 cut(s) 85, 172, 215, 395, 426, 537
Hpy188III TCNNGA 2 cut(s) 266, 436
Hpy99I CGWCG 4 cut(s) 70, 89, 209, 455
HpyAV CCTTC 2 cut(s) 62, 177
HpyCH4III ACNGT 2 cut(s) 19, 33
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 1 cut(s) 358
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 284, 299, 313, 350, 372, 476
HpyF3I CTNAG 4 cut(s) 184, 294, 303, 339
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 2 cut(s) 399, 447
Hsp92II CATG 2 cut(s) 223, 263
HspAI GCGC 5 cut(s) 283, 285, 287, 300, 447
KasI GGCGCC 1 cut(s) 446
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 4 cut(s) 8, 172, 252, 268
LpnPI CCDG 8 cut(s) 214, 275, 285, 326, 326, 330, 344, 457
MaeI CTAG 2 cut(s) 480, 491
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 4 cut(s) 19, 162, 472, 506
MalI GATC 4 cut(s) 10, 174, 254, 270
MboI GATC 4 cut(s) 8, 172, 252, 268
MboII GAAGA 1 cut(s) 137
MhlI GDGCHC 2 cut(s) 213, 393
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 1 cut(s) 368
MluNI TGGCCA 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 447
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 5 cut(s) 53, 91, 151, 343, 348
Mox20I TGGCCA 1 cut(s) 200
MreI CGCCGGCG 1 cut(s) 443
MroNI GCCGGC 1 cut(s) 443
MscI TGGCCA 1 cut(s) 200
MseI TTAA 4 cut(s) 330, 495, 528, 550
MslI CAYNNNNRTG 1 cut(s) 31
Msp20I TGGCCA 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 527
MspI CCGG 3 cut(s) 313, 317, 444
MvnI CGCG 2 cut(s) 285, 287
MwoI GCNNNNNNNGC 7 cut(s) 197, 284, 299, 313, 350, 372, 476
NaeI GCCGGC 1 cut(s) 445
NarI GGCGCC 1 cut(s) 447
NdeII GATC 4 cut(s) 8, 172, 252, 268
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 2 cut(s) 223, 263
NlaIV GGNNCC 2 cut(s) 190, 448
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 3 cut(s) 19, 162, 506
NspI RCATGY 1 cut(s) 263
PauI GCGCGC 2 cut(s) 283, 285
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 399, 447
PdiI GCCGGC 1 cut(s) 445
PfeI GAWTC 2 cut(s) 416, 432
PkrI GCNGC 3 cut(s) 193, 277, 365
PluTI GGCGCC 1 cut(s) 450
PspN4I GGNNCC 2 cut(s) 190, 448
PteI GCGCGC 2 cut(s) 283, 285
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 4 cut(s) 330, 495, 528, 550
SatI GCNGC 3 cut(s) 192, 276, 364
Sau3AI GATC 4 cut(s) 8, 172, 252, 268
SduI GDGCHC 2 cut(s) 213, 393
SetI ASST 8 cut(s) 93, 132, 169, 190, 309, 464, 492, 513
SfoI GGCGCC 1 cut(s) 448
SgrAI CRCCGGYG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 1 cut(s) 368
SsiI CCGC 3 cut(s) 192, 275, 363
SspDI GGCGCC 1 cut(s) 446
SspI AATATT 1 cut(s) 334
SspMI CTAG 2 cut(s) 480, 491
TaaI ACNGT 2 cut(s) 19, 33
TaiI ACGT 1 cut(s) 513
TaqI TCGA 1 cut(s) 96
TaqII GACCGA 1 cut(s) 239
TasI AATT 1 cut(s) 368
TauI GCSGC 3 cut(s) 194, 278, 366
TfiI GAWTC 2 cut(s) 416, 432
Tru1I TTAA 4 cut(s) 330, 495, 528, 550
Tru9I TTAA 4 cut(s) 330, 495, 528, 550
TscAI CASTG 1 cut(s) 302
TseFI GTSAC 3 cut(s) 19, 162, 506
Tsp45I GTSAC 3 cut(s) 19, 162, 506
TspDTI ATGAA 2 cut(s) 17, 471
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 527
XapI RAATTY 1 cut(s) 368
XceI RCATGY 1 cut(s) 263
XspI CTAG 2 cut(s) 480, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.