Rh4DG266300

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014918
Physical Location & Seq
Reverse (-)
48554205 .. 48554534
330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG266300.1

Sequence Viewer

Length: 330 bp
ATGGCTGATCTTGTTCTGTCCAATGCAACTGATACACTGGATTACATCCATGGCCTACTCAAGCAATCCCCAGAAGCAGATTTGCAGAAGCCTTTGGCAAACTGTGCAGAGTTGTACATTCCAGTGGTGAAGTACACTCTCCCACAGTCAATTGATGCTCTCAGTAATGGCCATTATGGGTTTGCCAACTACGGCATTTCTGATGCTGCAAAGGAAGCTGAGGCTTGTGAGAAGGGGTTTTCCGGGGAGTCGCCGTTGACAGATAGGAACACTCTTGTGAGCAGCCTCGCTGAAGTAGCTGTGGCTATCATCAAGTTAGTGCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

109

Amino Acids

11.58

Weight (kDa)

4.52

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 103 6.9e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 169
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 2 cut(s) 116, 134
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 2 cut(s) 218, 299
AluI AGCT 2 cut(s) 218, 299
AoxI GGCC 2 cut(s) 52, 169
ApeKI GCWGC 2 cut(s) 206, 282
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 139
BalI TGGCCA 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 193, 294
BceAI ACGGC 2 cut(s) 208, 238
BcnI CCSGG 1 cut(s) 244
BisI GCNGC 2 cut(s) 207, 283
BlsI GCNGC 2 cut(s) 208, 284
Bme1390I CCNGG 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 244
BmsI GCATC 2 cut(s) 145, 193
Bpu10I CCTNAGC 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 49, 243
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bse1I ACTGG 2 cut(s) 42, 122
BseDI CCNNGG 2 cut(s) 49, 243
BseGI GGATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 175, 210
BseNI ACTGG 2 cut(s) 42, 122
BseXI GCAGC 2 cut(s) 193, 294
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 2 cut(s) 54, 171
BsiSI CCGG 1 cut(s) 243
BsnI GGCC 2 cut(s) 54, 171
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 1 cut(s) 7
Bsp19I CCATGG 1 cut(s) 49
BspANI GGCC 2 cut(s) 54, 171
BspCNI CTCAG 2 cut(s) 174, 211
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 2 cut(s) 42, 122
BssECI CCNNGG 2 cut(s) 49, 243
BssMI GATC 1 cut(s) 7
BssT1I CCWWGG 1 cut(s) 49
Bst4CI ACNGT 2 cut(s) 104, 147
BstAPI GCANNNNNTGC 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 114
BstDEI CTNAG 2 cut(s) 161, 219
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 3 cut(s) 104, 215, 296
BstSCI CCNGG 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 193, 294
BsuRI GGCC 2 cut(s) 54, 171
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 35, 129
Csp6I GTAC 2 cut(s) 115, 133
CviAII CATG 1 cut(s) 50
CviJI RGCY 9 cut(s) 5, 54, 91, 171, 218, 224, 285, 299, 305
CviKI_1 RGCY 9 cut(s) 5, 54, 91, 171, 218, 224, 285, 299, 305
CviQI GTAC 2 cut(s) 115, 133
DdeI CTNAG 2 cut(s) 161, 219
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 1 cut(s) 53
FaiI YATR 2 cut(s) 51, 177
FatI CATG 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 207, 283
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 207, 283
GluI GCNGC 2 cut(s) 207, 283
HaeIII GGCC 2 cut(s) 54, 171
HapII CCGG 1 cut(s) 243
Hin1II CATG 1 cut(s) 53
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 1 cut(s) 248
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 135, 258
Hpy188I TCNGA 1 cut(s) 202
Hpy8I GTNNAC 2 cut(s) 135, 258
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 104, 147
HpyCH4V TGCA 5 cut(s) 26, 85, 107, 209, 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 215, 296
HpyF3I CTNAG 2 cut(s) 161, 219
Hsp92II CATG 1 cut(s) 53
Kzo9I GATC 1 cut(s) 7
LpnPI CCDG 5 cut(s) 23, 84, 135, 256, 308
Lsp1109I GCAGC 2 cut(s) 193, 294
LweI GCATC 2 cut(s) 145, 193
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MfeI CAATTG 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 1 cut(s) 150
MluNI TGGCCA 1 cut(s) 171
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 2 cut(s) 214, 296
Mox20I TGGCCA 1 cut(s) 171
MscI TGGCCA 1 cut(s) 171
MslI CAYNNNNRTG 2 cut(s) 122, 275
Msp20I TGGCCA 1 cut(s) 171
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 244
MunI CAATTG 1 cut(s) 150
MwoI GCNNNNNNNGC 3 cut(s) 104, 215, 296
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 49
NdeII GATC 1 cut(s) 7
NlaIII CATG 1 cut(s) 53
OliI CACNNNNGTG 1 cut(s) 275
PkrI GCNGC 2 cut(s) 208, 284
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
RsaI GTAC 2 cut(s) 116, 134
RsaNI GTAC 2 cut(s) 115, 133
RseI CAYNNNNRTG 2 cut(s) 122, 275
SatI GCNGC 2 cut(s) 207, 283
Sau3AI GATC 1 cut(s) 7
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 244
SetI ASST 2 cut(s) 220, 301
SfaNI GCATC 2 cut(s) 145, 193
SmiMI CAYNNNNRTG 2 cut(s) 122, 275
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 104, 147
TasI AATT 1 cut(s) 150
TatI WGTACW 2 cut(s) 114, 132
TscAI CASTG 2 cut(s) 42, 129
TseI GCWGC 2 cut(s) 206, 282
TspRI CASTG 2 cut(s) 42, 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.