FvH4_4g20180

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014918
Physical Location & Seq
Forward (+)
23527894 .. 23529143
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20180.t1

Sequence Viewer

Length: 525 bp
ATGAAGTACTCAATCCAAACCCCTCTTCTTGTTCTTGTTCATCTTGTTTTCCTCATTGTGCAAACCCAATCCCAAAGCACTGATCTTCTATCCAAAACATGCAAGAAGACACCCTACTATGACCTTTGTCTCTCATCTCTCCAGTCAAGCCCTCAGAGCTCAAACTCAGACATTGCAGGCCTGGCTCACATAATGGCTGATCTTGTTCTGTCCAATGCAACTGATACACTGGATTACATCCATAGCCTACTCAAGCAATCACCAGAAGCAGATTTGCAGAAGCCTTTGGCAAACTGTGCAGAGTTGTACATTCCAGTGGTCAAGTACACTCTCCCACAGTCAATTGATGCTCTCAGTAATGGCCATTATGGGTTTGCCAACTATGGCATTTCTGATGCTGCAAAGGAAGCTCAGGCTTGTGAGAAGGGGTTTTCAGGGTCAACCAAGCCATTGACCGATAGGAACACTCTTGTGAGCTGCCTCTCTGAAGTGGCTGTGGCCATCATCAATTTAGTGCTGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

175

Amino Acids

18.75

Weight (kDa)

5.42

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 168 9.9e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 361, 498
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 3 cut(s) 8, 308, 326
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AleI CACNNNNGTG 1 cut(s) 470
AluBI AGCT 3 cut(s) 159, 410, 477
AluI AGCT 3 cut(s) 159, 410, 477
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 1 cut(s) 134
AoxI GGCC 3 cut(s) 178, 361, 498
ApeKI GCWGC 2 cut(s) 398, 477
AsuHPI GGTGA 1 cut(s) 252
BalI TGGCCA 2 cut(s) 363, 500
BanII GRGCYC 1 cut(s) 161
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BbsI GAAGAC 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 385, 464
BccI CCATC 1 cut(s) 509
BciT130I CCWGG 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 134
BisI GCNGC 2 cut(s) 399, 478
BlsI GCNGC 2 cut(s) 400, 479
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 182
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 2 cut(s) 337, 385
BoxI GACNNNNGTC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 113
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 411
BpuEI CTTGAG 1 cut(s) 236
Bse1I ACTGG 3 cut(s) 142, 234, 314
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 182
BseGI GGATG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 4 cut(s) 167, 180, 367, 425
BseNI ACTGG 3 cut(s) 142, 234, 314
BseXI GCAGC 2 cut(s) 385, 464
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 318
BshFI GGCC 3 cut(s) 180, 363, 500
BsiHKAI GWGCWC 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 134
BsnI GGCC 3 cut(s) 180, 363, 500
Bsp1286I GDGCHC 1 cut(s) 161
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 2 cut(s) 82, 199
BspANI GGCC 3 cut(s) 180, 363, 500
BspCNI CTCAG 4 cut(s) 166, 179, 366, 424
BsrDI GCAATG 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 3 cut(s) 142, 234, 314
BssMI GATC 2 cut(s) 82, 199
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 296, 339
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 296
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 4 cut(s) 153, 166, 353, 411
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 2 cut(s) 85, 202
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 82, 199
BstMWI GCNNNNNNNGC 4 cut(s) 156, 182, 296, 407
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 102
BstPAI GACNNNNGTC 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 385, 464
BstV2I GAAGAC 1 cut(s) 113
BsuRI GGCC 3 cut(s) 180, 363, 500
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 3 cut(s) 78, 227, 321
Cac8I GCNNGC 1 cut(s) 178
Csp6I GTAC 3 cut(s) 7, 307, 325
CviAII CATG 1 cut(s) 99
CviQI GTAC 3 cut(s) 7, 307, 325
DdeI CTNAG 4 cut(s) 153, 166, 353, 411
DpnI GATC 2 cut(s) 84, 201
DpnII GATC 2 cut(s) 82, 199
EaeI YGGCCR 2 cut(s) 361, 498
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 161
FaeI CATG 1 cut(s) 102
FaiI YATR 6 cut(s) 100, 120, 191, 243, 369, 384
FatI CATG 1 cut(s) 98
Fnu4HI GCNGC 2 cut(s) 399, 478
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 399, 478
GluI GCNGC 2 cut(s) 399, 478
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 3 cut(s) 180, 363, 500
Hin1II CATG 1 cut(s) 102
HincII GTYRAC 1 cut(s) 441
HindII GTYRAC 1 cut(s) 441
HphI GGTGA 1 cut(s) 252
Hpy166II GTNNAC 2 cut(s) 327, 441
Hpy188I TCNGA 4 cut(s) 156, 169, 394, 487
Hpy8I GTNNAC 2 cut(s) 327, 441
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 296, 339
HpyCH4V TGCA 7 cut(s) 61, 102, 176, 218, 277, 299, 401
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 182, 296, 407
HpyF3I CTNAG 4 cut(s) 153, 166, 353, 411
Hsp92II CATG 1 cut(s) 102
Kzo9I GATC 2 cut(s) 82, 199
Lsp1109I GCAGC 2 cut(s) 385, 464
LweI GCATC 2 cut(s) 337, 385
MalI GATC 2 cut(s) 84, 201
MboI GATC 2 cut(s) 82, 199
MboII GAAGA 3 cut(s) 17, 77, 118
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 161
MlsI TGGCCA 2 cut(s) 363, 500
MluCI AATT 2 cut(s) 342, 508
MluNI TGGCCA 2 cut(s) 363, 500
MnlI CCTC 4 cut(s) 33, 62, 162, 491
Mox20I TGGCCA 2 cut(s) 363, 500
MscI TGGCCA 2 cut(s) 363, 500
MslI CAYNNNNRTG 2 cut(s) 314, 470
Msp20I TGGCCA 2 cut(s) 363, 500
MspR9I CCNGG 1 cut(s) 182
MunI CAATTG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 156, 182, 296, 407
NdeII GATC 2 cut(s) 82, 199
NlaIII CATG 1 cut(s) 102
NspI RCATGY 1 cut(s) 102
OliI CACNNNNGTG 1 cut(s) 470
PceI AGGCCT 1 cut(s) 180
PkrI GCNGC 2 cut(s) 400, 479
PshAI GACNNNNGTC 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 1 cut(s) 180
RsaI GTAC 3 cut(s) 8, 308, 326
RsaNI GTAC 3 cut(s) 7, 307, 325
RseI CAYNNNNRTG 2 cut(s) 314, 470
SacI GAGCTC 1 cut(s) 161
SatI GCNGC 2 cut(s) 399, 478
Sau3AI GATC 2 cut(s) 82, 199
ScaI AGTACT 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 161
SetI ASST 4 cut(s) 126, 161, 412, 479
SfaNI GCATC 2 cut(s) 337, 385
SmiMI CAYNNNNRTG 2 cut(s) 314, 470
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 342, 508
SseBI AGGCCT 1 cut(s) 180
SstI GAGCTC 1 cut(s) 161
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 2 cut(s) 296, 339
TaqII GACCGA 1 cut(s) 470
TasI AATT 2 cut(s) 342, 508
TatI WGTACW 3 cut(s) 6, 306, 324
TscAI CASTG 3 cut(s) 85, 234, 321
TseI GCWGC 2 cut(s) 398, 477
TspDTI ATGAA 2 cut(s) 17, 29
TspRI CASTG 3 cut(s) 85, 234, 321
XceI RCATGY 1 cut(s) 102
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.