MD16G1150200.v1.1

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014918
Physical Location & Seq
Forward (+)
11837498 .. 11838049
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1150200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGAGTAGCTCAATCTCAAGATTCTCAACCCTTCCTTTTCTACTCATTGTTTTACTCATTGCACCCCCAACAACCCAATCATTTTTTCTTCCATCAGATGAAGGCAATGATCTGATACAAACAACATGCAAAAAAACACCCCACTATGATCTCTGCCTCTCAACCCTCCAATCAAACCCCGACAGCTCAAACGCCGACGTGCCGGGATTGGCCCGGATAGTATCCGATGCTCTACTGGCAAATGAAAGTGACACACTGGATTACATCCACGGCCTGCTGAAGCAGTCCCCGGAGGCAGATTTGCAGAAAGCATTGGCAAACTGTGCTGAGTTGTACATCCCAGTTGTGAAGTTTAGCCTGCCGCAGGCAATTGAAGCAATGGGAAATGGGCACTTTGGGTTTGCAAAGTACGGGATTTCTGATGCTGCAAAGGAGGCTGAGGCATGCGAGAAGGGTTTTGGAGGAGTGAAGTCTCCTCTCACTGATAGGAATGCTCTTGTGGATGATCTTTCTGGTGTGGCTGTTGCCATTCTCAATTTATTAAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

184

Amino Acids

19.44

Weight (kDa)

4.72

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 177 3.3e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 2 cut(s) 335, 410
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 1 cut(s) 374
AjiI CACGTC 1 cut(s) 199
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 2 cut(s) 9, 186
AluI AGCT 2 cut(s) 9, 186
Alw26I GTCTC 1 cut(s) 477
AoxI GGCC 2 cut(s) 210, 271
ApeKI GCWGC 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 3 cut(s) 204, 214, 290
BaeGI GKGCMC 1 cut(s) 393
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 412
BccI CCATC 1 cut(s) 100
BceAI ACGGC 1 cut(s) 286
BciVI GTATCC 1 cut(s) 232
BcnI CCSGG 3 cut(s) 204, 214, 290
BcoDI GTCTC 1 cut(s) 477
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 2 cut(s) 362, 426
BlsI GCNGC 2 cut(s) 363, 427
Bme1390I CCNGG 3 cut(s) 204, 214, 290
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 211
BmrFI CCNGG 3 cut(s) 204, 214, 290
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 217, 412
BmuI ACTGGG 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 438
BpuMI CCSGG 3 cut(s) 204, 214, 290
BsaJI CCNNGG 2 cut(s) 268, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 3 cut(s) 240, 261, 341
Bse3DI GCAATG 3 cut(s) 57, 112, 384
BseDI CCNNGG 2 cut(s) 268, 288
BseGI GGATG 3 cut(s) 264, 336, 508
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMI GCAATG 3 cut(s) 57, 112, 384
BseMII CTCAG 2 cut(s) 318, 429
BseNI ACTGG 3 cut(s) 240, 261, 341
BseRI GAGGAG 2 cut(s) 465, 477
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 2 cut(s) 212, 273
BsiSI CCGG 3 cut(s) 203, 214, 290
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 2 cut(s) 212, 273
Bsp1286I GDGCHC 1 cut(s) 393
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 3 cut(s) 109, 148, 505
BspACI CCGC 1 cut(s) 362
BspANI GGCC 2 cut(s) 212, 273
BspCNI CTCAG 2 cut(s) 319, 430
BsrDI GCAATG 3 cut(s) 57, 112, 384
BsrGI TGTACA 1 cut(s) 333
BsrI ACTGG 3 cut(s) 240, 261, 341
BssECI CCNNGG 2 cut(s) 268, 288
BssMI GATC 3 cut(s) 109, 148, 505
Bst4CI ACNGT 1 cut(s) 323
BstAPI GCANNNNNTGC 1 cut(s) 323
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 4 cut(s) 275, 359, 366, 445
BstDEI CTNAG 2 cut(s) 327, 438
BstDSI CCRYGG 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 3 cut(s) 264, 336, 508
BstKTI GATC 3 cut(s) 112, 151, 508
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 3 cut(s) 109, 148, 505
BstMWI GCNNNNNNNGC 4 cut(s) 236, 323, 374, 434
BstNSI RCATGY 2 cut(s) 129, 447
BstSCI CCNGG 3 cut(s) 202, 212, 288
BstSLI GKGCMC 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 412
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 2 cut(s) 212, 273
BtgI CCRYGG 1 cut(s) 268
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 3 cut(s) 264, 336, 508
BtsIMutI CAGTG 2 cut(s) 254, 480
Cac8I GCNNGC 4 cut(s) 275, 359, 366, 445
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 334, 409
CviAII CATG 2 cut(s) 126, 444
CviJI RGCY 7 cut(s) 9, 186, 212, 273, 357, 437, 521
CviKI_1 RGCY 7 cut(s) 9, 186, 212, 273, 357, 437, 521
CviQI GTAC 2 cut(s) 334, 409
DdeI CTNAG 2 cut(s) 327, 438
DpnI GATC 3 cut(s) 111, 150, 507
DpnII GATC 3 cut(s) 109, 148, 505
Eco57I CTGAAG 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 362
FaeI CATG 2 cut(s) 129, 447
FaiI YATR 3 cut(s) 127, 147, 445
FaqI GGGAC 1 cut(s) 271
FatI CATG 2 cut(s) 125, 443
Fnu4HI GCNGC 2 cut(s) 362, 426
FokI GGATG 3 cut(s) 251, 323, 515
Fsp4HI GCNGC 2 cut(s) 362, 426
GluI GCNGC 2 cut(s) 362, 426
HaeIII GGCC 2 cut(s) 212, 273
HapII CCGG 3 cut(s) 203, 214, 290
Hin1II CATG 2 cut(s) 129, 447
HinfI GANTC 1 cut(s) 21
HpaII CCGG 3 cut(s) 203, 214, 290
Hpy188I TCNGA 4 cut(s) 97, 114, 226, 421
Hpy188III TCNNGA 1 cut(s) 18
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 3 cut(s) 41, 95, 445
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 5 cut(s) 62, 129, 304, 404, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 323, 374, 434
HpyF3I CTNAG 2 cut(s) 327, 438
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 2 cut(s) 129, 447
Kzo9I GATC 3 cut(s) 109, 148, 505
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 217, 412
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 111, 150, 507
MboI GATC 3 cut(s) 109, 148, 505
MboII GAAGA 1 cut(s) 80
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 2 cut(s) 369, 535
MnlI CCTC 7 cut(s) 167, 176, 286, 427, 433, 455, 486
MseI TTAA 1 cut(s) 542
MspI CCGG 3 cut(s) 203, 214, 290
MspR9I CCNGG 3 cut(s) 204, 214, 290
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 236, 323, 374, 434
NciI CCSGG 3 cut(s) 204, 214, 290
NdeII GATC 3 cut(s) 109, 148, 505
NlaIII CATG 2 cut(s) 129, 447
NmuCI GTSAC 1 cut(s) 248
NspI RCATGY 2 cut(s) 129, 447
PaeI GCATGC 1 cut(s) 447
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 2 cut(s) 363, 427
PspPI GGNCC 1 cut(s) 211
RsaI GTAC 2 cut(s) 335, 410
RsaNI GTAC 2 cut(s) 334, 409
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 2 cut(s) 362, 426
Sau3AI GATC 3 cut(s) 109, 148, 505
Sau96I GGNCC 1 cut(s) 211
ScrFI CCNGG 3 cut(s) 204, 214, 290
SduI GDGCHC 1 cut(s) 393
SetI ASST 3 cut(s) 11, 188, 201
SfaNI GCATC 2 cut(s) 217, 412
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
SphI GCATGC 1 cut(s) 447
Sse9I AATT 2 cut(s) 369, 535
SsiI CCGC 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 202, 212, 288
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 201
TasI AATT 2 cut(s) 369, 535
TatI WGTACW 1 cut(s) 333
TauI GCSGC 1 cut(s) 364
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 2 cut(s) 261, 487
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 2 cut(s) 114, 258
TspRI CASTG 2 cut(s) 261, 487
XagI CCTNNNNNAGG 1 cut(s) 362
XceI RCATGY 2 cut(s) 129, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.