Rorug04G0207000

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014918
Physical Location & Seq
Forward (+)
36039543 .. 36043777
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0207000.1

Sequence Viewer

Length: 2733 bp
ATGAACGCACCCCCGTTTAACTCCTCCTGCGGCCCATGGAGCCAGCCACCGCCGCCGCCACTTCCCCTAAATCCCAATTTATCCCTTCAAAACCCCCTCAATCTATACATGTACCTTTCAAACCTCGCCTCCTTCACACCCCACTACCACCCACCTTTCCCTCCCAATAATTTCTCAATCCAAAACCCTAGCTTCGCCCCCAAGCACTTCTCCAATTCAGCATTTCATCCTCAGGCTCAGGCCTCGTCCCAGAACTCGAAAGAGCAGCTTGAGAGAATCGACCGCGCCGTGGAGAAGGCTCGCTCCGACCTTATTGCCGCCGGGGAAAGCGTCTCGGCGTGGAAGGTCTCGCAGTCTGCGATTCGGATTCTGAAGGTTGATTGCTGGAGCTCTCTAGGGTTTCAAATGCAGCAGGTGCCGTCTCTGCACCGCCTCATGCTCACTCAAGGCAAGATAGATGCATTTATCCATTGTTTTGTTGGGGTTCGAAGAATTGCTTCATTGTATGATCTCGAAGTAGCAATCTGCGAGAATGAGGGCGTTGAACGGTTTGAAGAGCTGGGATTGGGCCCTTTGTTGCGAAATCCTCTTGTCGTGCATTATTTCTCAGTGAAGTGTGATGCAACTGAAGTGTTTAGGATAACGAGTGAGGAGATAATATCTTTACTTTCTGAGTTCATGGATGCTTGTAAACATACATATATAAGGGTTGAAGAATTTTTAGATTTCATTGTAAAGAAGTGTGCAGTTGCAAGCAGAGAAGAGCTTGGAATACGAATTCACAGCTTGGGGATGCATATTTCAGCCATCCGGGTTGCTAGGGATTTAGAATCTGCTGCTCTGAAGAAATCTGGAGAGTTAATTATTCAACAACCAGATTCAAGATGTATGAAGCAACCAATGAGAATGCAGCAAGACGAACATTTTACCACCTCCATACGTAGACAGTTTTGTGGCAAGCACATTAGGTTTGATTCAGAGGATGAATGTAGTGATGACGAAAATAATGACCATGTCAGAAACAACCATGTTAACTTATCTTCACAATCTGCTAAAAGTTCTGACAGAGTGGACAGTGATGATGGTTTGGTTGTCTCTCCATCACAATCCAAGGGTCCACTCAACCAGGTTAACATATCATCACAATCAGCTGAAAGTTGTGACAGACTGAACACTGATGATGGTTTGGTTGTCTCTCCATCACAATCCAAAGGTCCACTCCCCTCATTGAAAATGCAGCAAGACAAAGGTTTCAGTACCATTTCTTGTCGTGTTGATTCATTTTTCTCTGTACATAAGCAGTTTTGTGGCAAGCACACTAGGTTTGATTTGGAGGATGAAGGTAGTGATGTTGAAAATAATGACCATGTCAGAAGCAACCATGTTAACTTATCTTCACAATCTGCTAAAAGTTCTGACAGAGTGGACTGTGATGATGGTTTGGTTGTCTCTCCATCACAATCTAAGGGTCCGCTCAACCAGGTTGACTTATCATCGCAATCTGCTGAAAGTTTTGACAGAGTGAACACTGATGATGGTTTGGTGGTCTCTCCATCACAATCCAAAGGTCCACTTCCCTCATTGAAAATGCAGCAAGACAAAGGTTTCAGTACCATTTCTTGTCATGTTGATTCATTTTTCTCTGTAAATAAGCAGTTTTGTGGCAAGCACATTAGGTTTGATTCTGCAGAAAACTCGGAGGATGAATGTATCGATGATGATTTACATGATGAAGAAGATTATAATCGTGACCAGGTCACATGCAGTGTGGTTAACATCTCTTCACAATCTGGTGGAAGGTCTGACCGTGTGAGCAGCTGTCCTTACCCATCTGCAATAGAAGAGATACAACGGCTTGGGTTGGACCAGCTTTCCCCAGATAGTGGCAGCCAAAAGCACAAAGGGCCTAATGACTCAGTTAAAAAGAAGAGAAAATCTGAAATTCCTCTAAATGCAATCTCTATGCCCCCCAAATTGTGTAGGACAGAAAAGGTAGAACAAGTTGCTCAGCCAATAGAGAATGGCAGGGGAGCCAATAATGTGAGAAATTTGGATGGAAGGAAAGCGGAAAGCTGGCCACCTGTTGATTGGAGAACTGCACCAAGGTATGATTATGCTTGTGCAAATGGCTTTAGGACACGGGCACCAATTTTACAGCCTAGAAGTGACTTACAAAAGAAGGGGGATGATAATTATGATGGATTTGTTAAACGGACGGATAATATGATTCCTGTTACAGATACTCCTCAGTGGGGCACATCTAACTTTGGCAAGAGAGATCGTCTTAGTACTTGCACACCTGATGCAATACAAGCAAAGGAAACTGGGAGACTAGGTGAGCTTGCTGCTTTTAAATACTTCATTGAGAAAGCTGGCAAGTCAGCTGTGAAGTGGGTTAATGAGCATAGTGAAACAAGGCTACCGTATGATATAGTGGTAGGGGAGAATGAAGACAGAAAAGAGTTCATTGAAGTTAAAGCAACAAAAAATCCAACCAAAGACTGGTTTGAAATGTCAGTGAGACAGTTGCAATTTGCAGTTGAGCAAGGTGAAGCTTTCAGTATTGCTCATGTCATTTTATCAGATTATAATGTTGCGACAGTGAGAGTGTACAACAATCTGGAGAAACTGCTGATGAAGCATAACAACCAGGAGCTACAGGACCAGGATTGCAAGATGAAGTTGGTTGTCTTGGTGCTTGAGCCGCAGAAGGAATTTACCATTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

910

Amino Acids

101.99

Weight (kDa)

5.78

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOV_C PF13020 779 - 860 1e-17 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1743, 2589
AarI CACCTGC 1 cut(s) 403
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 2 cut(s) 415, 2143
AccB7I CCANNNNNTGG 2 cut(s) 1883, 2502
AccBSI CCGCTC 1 cut(s) 1474
AccI GTMKAC 1 cut(s) 943
AccII CGCG 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 2075
AcsI RAATTY 5 cut(s) 716, 777, 1941, 2047, 2714
AcuI CTGAAG 3 cut(s) 392, 648, 863
AfaI GTAC 6 cut(s) 113, 1258, 1293, 1612, 2290, 2612
AfiI CCNNNNNNNGG 4 cut(s) 289, 1883, 2252, 2502
AflIII ACRYGT 1 cut(s) 108
AjnI CCWGG 5 cut(s) 1125, 1479, 1752, 2649, 2664
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 9 cut(s) 337, 352, 426, 1099, 1198, 1453, 1552, 2323, 2515
AoxI GGCC 5 cut(s) 31, 240, 568, 1904, 2075
ApaI GGGCCC 1 cut(s) 572
ApeKI GCWGC 9 cut(s) 265, 409, 836, 910, 1237, 1591, 1815, 1887, 2345
ApoI RAATTY 5 cut(s) 716, 777, 1941, 2047, 2714
Asp700I GAANNNNTTC 1 cut(s) 496
AspLEI GCGC 1 cut(s) 287
AsuC2I CCSGG 2 cut(s) 322, 812
AsuHPI GGTGA 2 cut(s) 2348, 2561
AsuII TTCGAA 1 cut(s) 487
AvaII GGWCC 6 cut(s) 1115, 1214, 1469, 1568, 1864, 2662
AxyI CCTNAGG 1 cut(s) 231
BaeGI GKGCMC 3 cut(s) 572, 2146, 2258
BalI TGGCCA 1 cut(s) 2077
BanI GGYRCC 2 cut(s) 415, 2143
BanII GRGCYC 2 cut(s) 392, 572
BbsI GAAGAC 1 cut(s) 2457
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 9 cut(s) 277, 421, 823, 922, 1249, 1603, 1827, 1899, 2332
BceAI ACGGC 3 cut(s) 272, 403, 1868
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 1677, 1711
BciT130I CCWGG 5 cut(s) 1127, 1481, 1754, 2651, 2666
BcnI CCSGG 2 cut(s) 322, 812
BcoDI GTCTC 9 cut(s) 337, 352, 426, 1099, 1198, 1453, 1552, 2323, 2515
BfaI CTAG 6 cut(s) 189, 395, 819, 1320, 2160, 2333
BfmI CTRYAG 2 cut(s) 1686, 2657
BfuAI ACCTGC 1 cut(s) 403
BlpI GCTNAGC 1 cut(s) 2007
BmcAI AGTACT 1 cut(s) 2290
Bme1390I CCNGG 7 cut(s) 322, 812, 1127, 1481, 1754, 2651, 2666
Bme18I GGWCC 6 cut(s) 1115, 1214, 1469, 1568, 1864, 2662
BmiI GGNNCC 7 cut(s) 41, 417, 570, 1116, 1470, 2032, 2145
BmrFI CCNGG 7 cut(s) 322, 812, 1127, 1481, 1754, 2651, 2666
BmrI ACTGGG 1 cut(s) 2334
BmsI GCATC 5 cut(s) 448, 610, 673, 783, 2293
BmuI ACTGGG 1 cut(s) 2334
BpiI GAAGAC 1 cut(s) 2457
BpmI CTGGAG 3 cut(s) 406, 873, 2642
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 2007
Bpu14I TTCGAA 1 cut(s) 487
BpuEI CTTGAG 3 cut(s) 290, 429, 2720
BpuMI CCSGG 2 cut(s) 322, 812
Bsa29I ATCGAT 1 cut(s) 1713
BsaAI YACGTR 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 1743
BsaI GGTCTC 2 cut(s) 352, 1552
BsaJI CCNNGG 5 cut(s) 35, 288, 321, 1110, 2102
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 2227, 2257, 2320, 2350
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 1883, 2252, 2502
Bse1I ACTGG 2 cut(s) 2329, 2507
Bse21I CCTNAGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 1743
BseBI CCWGG 5 cut(s) 1127, 1481, 1754, 2651, 2666
BseCI ATCGAT 1 cut(s) 1713
BseDI CCNNGG 5 cut(s) 35, 288, 321, 1110, 2102
BseGI GGATG 9 cut(s) 226, 688, 798, 807, 988, 1342, 1708, 2059, 2191
BseJI GATNNNNATC 1 cut(s) 1743
BseLI CCNNNNNNNGG 4 cut(s) 289, 1883, 2252, 2502
BseMII CTCAG 7 cut(s) 245, 251, 621, 663, 1929, 2021, 2261
BseNI ACTGG 2 cut(s) 2329, 2507
BseRI GAGGAG 3 cut(s) 13, 665, 2235
BseSI GKGCMC 3 cut(s) 572, 2146, 2258
BseXI GCAGC 9 cut(s) 277, 421, 823, 922, 1249, 1603, 1827, 1899, 2332
BseYI CCCAGC 1 cut(s) 559
BsgI GTGCAG 3 cut(s) 410, 765, 2082
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 5 cut(s) 33, 242, 570, 1906, 2077
BshNI GGYRCC 2 cut(s) 415, 2143
BshVI ATCGAT 1 cut(s) 1713
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 2 cut(s) 321, 811
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 4 cut(s) 289, 1883, 2252, 2502
BsmAI GTCTC 9 cut(s) 337, 352, 426, 1099, 1198, 1453, 1552, 2323, 2515
BsmBI CGTCTC 2 cut(s) 337, 426
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 912
BsnI GGCC 5 cut(s) 33, 242, 570, 1906, 2077
Bso31I GGTCTC 2 cut(s) 352, 1552
Bsp119I TTCGAA 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 568
Bsp1286I GDGCHC 4 cut(s) 392, 572, 2146, 2258
Bsp1407I TGTACA 2 cut(s) 1291, 2610
Bsp143I GATC 2 cut(s) 508, 2278
Bsp1720I GCTNAGC 1 cut(s) 2007
Bsp19I CCATGG 1 cut(s) 35
BspANI GGCC 5 cut(s) 33, 242, 570, 1906, 2077
BspCNI CTCAG 7 cut(s) 244, 250, 620, 664, 1928, 2020, 2260
BspDI ATCGAT 1 cut(s) 1713
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 7 cut(s) 41, 417, 570, 1116, 1470, 2032, 2145
BspMAI CTGCAG 1 cut(s) 1690
BspMI ACCTGC 1 cut(s) 403
BspQI GCTCTTC 2 cut(s) 549, 756
BspT104I TTCGAA 1 cut(s) 487
BspT107I GGYRCC 2 cut(s) 415, 2143
BspTNI GGTCTC 2 cut(s) 352, 1552
BsrBI CCGCTC 1 cut(s) 1474
BsrGI TGTACA 2 cut(s) 1291, 2610
BsrI ACTGG 2 cut(s) 2329, 2507
BssECI CCNNGG 5 cut(s) 35, 288, 321, 1110, 2102
BssMI GATC 2 cut(s) 508, 2278
BssT1I CCWWGG 3 cut(s) 35, 1110, 2102
Bst2UI CCWGG 5 cut(s) 1127, 1481, 1754, 2651, 2666
Bst4CI ACNGT 8 cut(s) 549, 948, 1076, 1430, 1808, 2424, 2526, 2602
Bst6I CTCTTC 5 cut(s) 549, 756, 1786, 1836, 1922
BstAPI GCANNNNNTGC 1 cut(s) 415
BstAUI TGTACA 2 cut(s) 1291, 2610
BstBAI YACGTR 1 cut(s) 941
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 9 cut(s) 44, 301, 754, 959, 1313, 1667, 2075, 2343, 2374
BstDEI CTNAG 9 cut(s) 231, 237, 607, 672, 1464, 1915, 2007, 2247, 2285
BstDSI CCRYGG 2 cut(s) 35, 288
BstF5I GGATG 9 cut(s) 226, 688, 798, 807, 988, 1342, 1708, 2059, 2191
BstFNI CGCG 1 cut(s) 285
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 2 cut(s) 511, 2281
BstMAI GTCTC 9 cut(s) 337, 352, 426, 1099, 1198, 1453, 1552, 2323, 2515
BstMBI GATC 2 cut(s) 508, 2278
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 8 cut(s) 39, 52, 415, 424, 1903, 2312, 2638, 2704
BstNI CCWGG 5 cut(s) 1127, 1481, 1754, 2651, 2666
BstNSI RCATGY 2 cut(s) 112, 1764
BstSCI CCNGG 7 cut(s) 320, 810, 1125, 1479, 1752, 2649, 2664
BstSFI CTRYAG 2 cut(s) 1686, 2657
BstSLI GKGCMC 3 cut(s) 572, 2146, 2258
BstSNI TACGTA 1 cut(s) 941
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 9 cut(s) 277, 421, 823, 922, 1249, 1603, 1827, 1899, 2332
BstV2I GAAGAC 1 cut(s) 2457
Bsu15I ATCGAT 1 cut(s) 1713
Bsu36I CCTNAGG 1 cut(s) 231
BsuRI GGCC 5 cut(s) 33, 242, 570, 1906, 2077
BsuTUI ATCGAT 1 cut(s) 1713
BtgI CCRYGG 2 cut(s) 35, 288
BtgZI GCGATG 1 cut(s) 1479
BtsCI GGATG 9 cut(s) 226, 688, 798, 807, 988, 1342, 1708, 2059, 2191
BtsI GCAGTG 1 cut(s) 1771
BtsIMutI CAGTG 8 cut(s) 615, 1081, 1173, 1527, 1771, 2255, 2523, 2607
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 9 cut(s) 44, 301, 754, 959, 1313, 1667, 2075, 2343, 2374
CfoI GCGC 1 cut(s) 287
ClaI ATCGAT 1 cut(s) 1713
CseI GACGC 1 cut(s) 319
CsiI ACCWGGT 3 cut(s) 1125, 1479, 1752
Csp6I GTAC 6 cut(s) 112, 1257, 1292, 1611, 2289, 2611
CviQI GTAC 6 cut(s) 112, 1257, 1292, 1611, 2289, 2611
DdeI CTNAG 9 cut(s) 231, 237, 607, 672, 1464, 1915, 2007, 2247, 2285
DpnI GATC 2 cut(s) 510, 2280
DpnII GATC 2 cut(s) 508, 2278
DraI TTTAAA 1 cut(s) 2353
EaeI YGGCCR 1 cut(s) 2075
Eam1104I CTCTTC 5 cut(s) 549, 756, 1786, 1836, 1922
EarI CTCTTC 5 cut(s) 549, 756, 1786, 1836, 1922
Ecl136II GAGCTC 1 cut(s) 390
Eco105I TACGTA 1 cut(s) 941
Eco130I CCWWGG 3 cut(s) 35, 1110, 2102
Eco147I AGGCCT 1 cut(s) 242
Eco24I GRGCYC 2 cut(s) 392, 572
Eco31I GGTCTC 2 cut(s) 352, 1552
Eco47I GGWCC 6 cut(s) 1115, 1214, 1469, 1568, 1864, 2662
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 3 cut(s) 392, 648, 863
Eco81I CCTNAGG 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 390
EcoO109I RGGNCCY 2 cut(s) 569, 1904
EcoRI GAATTC 1 cut(s) 777
EcoRII CCWGG 5 cut(s) 1125, 1479, 1752, 2649, 2664
EcoT14I CCWWGG 3 cut(s) 35, 1110, 2102
EcoT22I ATGCAT 2 cut(s) 463, 798
EcoT38I GRGCYC 2 cut(s) 392, 572
ErhI CCWWGG 3 cut(s) 35, 1110, 2102
Esp3I CGTCTC 2 cut(s) 337, 426
FalI AAGNNNNNCTT 6 cut(s) 252, 284, 481, 513, 1557, 1589
FaqI GGGAC 1 cut(s) 232
FblI GTMKAC 1 cut(s) 943
FokI GGATG 9 cut(s) 213, 695, 794, 805, 995, 1349, 1715, 2066, 2198
FriOI GRGCYC 2 cut(s) 392, 572
FspBI CTAG 6 cut(s) 189, 395, 819, 1320, 2160, 2333
GlaI GCGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 3 cut(s) 406, 873, 2642
HaeIII GGCC 5 cut(s) 33, 242, 570, 1906, 2077
HapII CCGG 2 cut(s) 321, 811
HgaI GACGC 1 cut(s) 319
HhaI GCGC 1 cut(s) 287
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 5 cut(s) 1033, 1132, 1387, 1486, 1774
HindII GTYRAC 5 cut(s) 1033, 1132, 1387, 1486, 1774
HindIII AAGCTT 1 cut(s) 2553
HpaI GTTAAC 4 cut(s) 1033, 1132, 1387, 1774
HpaII CCGG 2 cut(s) 321, 811
HphI GGTGA 2 cut(s) 2348, 2561
Hpy188III TCNNGA 6 cut(s) 512, 852, 882, 1748, 2621, 2730
HpyCH4III ACNGT 8 cut(s) 549, 948, 1076, 1430, 1808, 2424, 2526, 2602
HpyCH4IV ACGT 1 cut(s) 940
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 52, 415, 424, 1903, 2312, 2638, 2704
HpyF3I CTNAG 9 cut(s) 231, 237, 607, 672, 1464, 1915, 2007, 2247, 2285
HpySE526I ACGT 1 cut(s) 940
HspAI GCGC 1 cut(s) 285
KspAI GTTAAC 4 cut(s) 1033, 1132, 1387, 1774
Kzo9I GATC 2 cut(s) 508, 2278
LguI GCTCTTC 2 cut(s) 549, 756
LmnI GCTCC 5 cut(s) 39, 308, 387, 2030, 2653
Lsp1109I GCAGC 9 cut(s) 277, 421, 823, 922, 1249, 1603, 1827, 1899, 2332
LweI GCATC 5 cut(s) 448, 610, 673, 783, 2293
MabI ACCWGGT 3 cut(s) 1125, 1479, 1752
MaeI CTAG 6 cut(s) 189, 395, 819, 1320, 2160, 2333
MaeII ACGT 1 cut(s) 940
MaeIII GTNAC 5 cut(s) 1160, 1748, 1756, 2165, 2233
MalI GATC 2 cut(s) 510, 2280
MbiI CCGCTC 1 cut(s) 1474
MboI GATC 2 cut(s) 508, 2278
MhlI GDGCHC 4 cut(s) 392, 572, 2146, 2258
MlsI TGGCCA 1 cut(s) 2077
MluNI TGGCCA 1 cut(s) 2077
MlyI GAGTC 1 cut(s) 1907
MmeI TCCRAC 3 cut(s) 330, 1842, 2516
Mox20I TGGCCA 1 cut(s) 2077
Mph1103I ATGCAT 2 cut(s) 463, 798
MroXI GAANNNNTTC 1 cut(s) 496
MscI TGGCCA 1 cut(s) 2077
Msp20I TGGCCA 1 cut(s) 2077
MspA1I CMGCKG 3 cut(s) 1151, 1818, 2384
MspI CCGG 2 cut(s) 321, 811
MspR9I CCNGG 7 cut(s) 322, 812, 1127, 1481, 1754, 2651, 2666
Mva1269I GAATGC 1 cut(s) 912
MvaI CCWGG 5 cut(s) 1127, 1481, 1754, 2651, 2666
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 8 cut(s) 39, 52, 415, 424, 1903, 2312, 2638, 2704
NciI CCSGG 2 cut(s) 322, 812
NcoI CCATGG 1 cut(s) 35
NdeII GATC 2 cut(s) 508, 2278
NlaIV GGNNCC 7 cut(s) 41, 417, 570, 1116, 1470, 2032, 2145
NmeAIII GCCGAG 1 cut(s) 314
NmuCI GTSAC 4 cut(s) 1160, 1748, 1756, 2165
NsiI ATGCAT 2 cut(s) 463, 798
NspI RCATGY 2 cut(s) 112, 1764
NspV TTCGAA 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 403
PceI AGGCCT 1 cut(s) 242
PciI ACATGT 1 cut(s) 108
PciSI GCTCTTC 2 cut(s) 549, 756
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 356
PctI GAATGC 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 496
PflFI GACNNNGTC 3 cut(s) 1013, 1367, 1754
PflMI CCANNNNNTGG 2 cut(s) 1883, 2502
PleI GAGTC 1 cut(s) 1907
PpsI GAGTC 1 cut(s) 1907
Ppu21I YACGTR 1 cut(s) 941
PscI ACATGT 1 cut(s) 108
PsiI TTATAA 2 cut(s) 1743, 2589
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 5 cut(s) 1125, 1479, 1752, 2649, 2664
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 5 cut(s) 1125, 1479, 1752, 2649, 2664
PspN4I GGNNCC 7 cut(s) 41, 417, 570, 1116, 1470, 2032, 2145
PspOMI GGGCCC 1 cut(s) 568
PstI CTGCAG 1 cut(s) 1690
PsyI GACNNNGTC 3 cut(s) 1013, 1367, 1754
PvuII CAGCTG 3 cut(s) 1151, 1818, 2384
RsaI GTAC 6 cut(s) 113, 1258, 1293, 1612, 2290, 2612
RsaNI GTAC 6 cut(s) 112, 1257, 1292, 1611, 2289, 2611
SacI GAGCTC 1 cut(s) 392
SapI GCTCTTC 2 cut(s) 549, 756
Sau3AI GATC 2 cut(s) 508, 2278
ScaI AGTACT 1 cut(s) 2290
SchI GAGTC 1 cut(s) 1907
ScrFI CCNGG 7 cut(s) 322, 812, 1127, 1481, 1754, 2651, 2666
SduI GDGCHC 4 cut(s) 392, 572, 2146, 2258
SexAI ACCWGGT 3 cut(s) 1125, 1479, 1752
SfaNI GCATC 5 cut(s) 448, 610, 673, 783, 2293
SfcI CTRYAG 2 cut(s) 1686, 2657
SfuI TTCGAA 1 cut(s) 487
SinI GGWCC 6 cut(s) 1115, 1214, 1469, 1568, 1864, 2662
SmlI CTYRAG 3 cut(s) 269, 444, 2699
SmoI CTYRAG 3 cut(s) 269, 444, 2699
SnaBI TACGTA 1 cut(s) 941
SseBI AGGCCT 1 cut(s) 242
SspMI CTAG 6 cut(s) 189, 395, 819, 1320, 2160, 2333
SstI GAGCTC 1 cut(s) 392
StuI AGGCCT 1 cut(s) 242
StyD4I CCNGG 7 cut(s) 320, 810, 1125, 1479, 1752, 2649, 2664
StyI CCWWGG 3 cut(s) 35, 1110, 2102
TaaI ACNGT 8 cut(s) 549, 948, 1076, 1430, 1808, 2424, 2526, 2602
TaiI ACGT 1 cut(s) 943
TaqI TCGA 5 cut(s) 257, 279, 487, 513, 1713
TatI WGTACW 3 cut(s) 1291, 2288, 2610
TauI GCSGC 5 cut(s) 33, 55, 58, 320, 2707
TscAI CASTG 8 cut(s) 615, 1081, 1180, 1534, 1771, 2255, 2523, 2607
TseFI GTSAC 4 cut(s) 1160, 1748, 1756, 2165
TseI GCWGC 9 cut(s) 265, 409, 836, 910, 1237, 1591, 1815, 1887, 2345
Tsp45I GTSAC 4 cut(s) 1160, 1748, 1756, 2165
TspGWI ACGGA 2 cut(s) 2227, 2231
TspRI CASTG 8 cut(s) 615, 1081, 1180, 1534, 1771, 2255, 2523, 2607
Tth111I GACNNNGTC 3 cut(s) 1013, 1367, 1754
Van91I CCANNNNNTGG 2 cut(s) 1883, 2502
VpaK11BI GGWCC 6 cut(s) 1115, 1214, 1469, 1568, 1864, 2662
XapI RAATTY 5 cut(s) 716, 777, 1941, 2047, 2714
XceI RCATGY 2 cut(s) 112, 1764
XcmI CCANNNNNNNNNTGG 3 cut(s) 476, 2085, 2499
XmiI GTMKAC 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 496
XspI CTAG 6 cut(s) 189, 395, 819, 1320, 2160, 2333
ZrmI AGTACT 1 cut(s) 2290
Zsp2I ATGCAT 2 cut(s) 463, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.