MD13G1149800.v1.1

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014918
Physical Location & Seq
Forward (+)
11757213 .. 11757767
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1149800.v1.1.491

Sequence Viewer

Length: 555 bp
ATGAGTAGCTCACTCTCAAGATTCTCAACTCTTCTTCTTTTTCTCCACGTTGCTTTACTCATTGCACCGCCAACAACCCAATTTTTTTTTCTTCCATTAGCCAAAGGCAGTGATCTGATACAAACAACATGCAAAAAAACACCCCACTATAATCTCTGCCTCTCAACCCTCCAATCAAACCCCGACAGCTCAAACGCAGATGTGCCGGGATTGGCCCTGATAGCATCCGACGCTCTCCTGGCAAATGCAAGTGACACACTGGACTACATCCACGGCCTGCTGAAGCAATCCCCGGAAGTACATTTACAGAAGGCATTGGCAAACTGTGCTGAGCTGTACATCCCAATTGTGAAGTTTAGCCTGCCGCAGGCAATTGAAGCTTTGCGAAATGGGCACTTTGGGTTTGCAAAGTATGGGATTTCTGATGCTGCAAAGGAGGCTGAGGCATGTGAGAAGGGTTTTGGAGGAGTTGAGTCTCCTCTCACTGATAGGAATGCTCTTGTGAATGATCTTTCTGGTGTGGCTGTCGCCATTCTCAACTTATTAAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

185

Amino Acids

19.58

Weight (kDa)

5.91

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 178 5.6e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 68, 365
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 2 cut(s) 300, 338
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 1 cut(s) 377
AjnI CCWGG 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 4 cut(s) 9, 189, 334, 380
AluI AGCT 4 cut(s) 9, 189, 334, 380
Alw26I GTCTC 1 cut(s) 480
AoxI GGCC 2 cut(s) 213, 274
ApeKI GCWGC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 214
AsuC2I CCSGG 2 cut(s) 207, 293
BaeGI GKGCMC 1 cut(s) 396
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 415
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 2 cut(s) 207, 293
BcoDI GTCTC 1 cut(s) 480
BisI GCNGC 2 cut(s) 365, 429
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 2 cut(s) 366, 430
Bme1390I CCNGG 3 cut(s) 207, 239, 293
BmgT120I GGNCC 1 cut(s) 214
BmrFI CCNGG 3 cut(s) 207, 239, 293
BmsI GCATC 2 cut(s) 233, 415
Bpu10I CCTNAGC 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 207, 293
BsaJI CCNNGG 2 cut(s) 271, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 271, 291
BseGI GGATG 3 cut(s) 224, 267, 339
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 2 cut(s) 321, 432
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 2 cut(s) 468, 480
BseSI GKGCMC 1 cut(s) 396
BseXI GCAGC 1 cut(s) 415
BshFI GGCC 2 cut(s) 215, 276
BsiSI CCGG 2 cut(s) 206, 293
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 2 cut(s) 215, 276
Bsp1286I GDGCHC 1 cut(s) 396
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 2 cut(s) 112, 508
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 2 cut(s) 68, 365
BspANI GGCC 2 cut(s) 215, 276
BspCNI CTCAG 2 cut(s) 322, 433
BsrDI GCAATG 1 cut(s) 60
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 271, 291
BssMI GATC 2 cut(s) 112, 508
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 278, 362, 369
BstDEI CTNAG 2 cut(s) 330, 441
BstDSI CCRYGG 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 365
BstF5I GGATG 3 cut(s) 224, 267, 339
BstKTI GATC 2 cut(s) 115, 511
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 112, 508
BstMWI GCNNNNNNNGC 7 cut(s) 221, 230, 239, 326, 377, 391, 437
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 132, 450
BstSCI CCNGG 3 cut(s) 205, 237, 291
BstSLI GKGCMC 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 415
BsuRI GGCC 2 cut(s) 215, 276
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 3 cut(s) 224, 267, 339
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 3 cut(s) 115, 257, 483
Cac8I GCNNGC 3 cut(s) 278, 362, 369
Cfr13I GGNCC 1 cut(s) 214
CseI GACGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 299, 337
CviAII CATG 2 cut(s) 129, 447
CviQI GTAC 2 cut(s) 299, 337
DdeI CTNAG 2 cut(s) 330, 441
DpnI GATC 2 cut(s) 114, 510
DpnII GATC 2 cut(s) 112, 508
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 302
EcoNI CCTNNNNNAGG 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 237
FaeI CATG 2 cut(s) 132, 450
FaiI YATR 4 cut(s) 130, 150, 414, 448
FatI CATG 2 cut(s) 128, 446
Fnu4HI GCNGC 2 cut(s) 365, 429
FokI GGATG 3 cut(s) 211, 254, 326
Fsp4HI GCNGC 2 cut(s) 365, 429
GluI GCNGC 2 cut(s) 365, 429
HaeIII GGCC 2 cut(s) 215, 276
HapII CCGG 2 cut(s) 206, 293
HgaI GACGC 1 cut(s) 239
Hin1II CATG 2 cut(s) 132, 450
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 2 cut(s) 21, 473
HpaII CCGG 2 cut(s) 206, 293
Hpy188I TCNGA 3 cut(s) 117, 229, 424
Hpy188III TCNNGA 1 cut(s) 18
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 304, 448
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 5 cut(s) 65, 132, 248, 407, 431
HpyF10VI GCNNNNNNNGC 7 cut(s) 221, 230, 239, 326, 377, 391, 437
HpyF3I CTNAG 2 cut(s) 330, 441
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 2 cut(s) 132, 450
Kzo9I GATC 2 cut(s) 112, 508
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 233, 415
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 251
MalI GATC 2 cut(s) 114, 510
MboI GATC 2 cut(s) 112, 508
MboII GAAGA 3 cut(s) 23, 26, 83
MfeI CAATTG 2 cut(s) 345, 372
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 3 cut(s) 80, 345, 372
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 6 cut(s) 170, 179, 430, 436, 458, 489
MseI TTAA 1 cut(s) 545
MspI CCGG 2 cut(s) 206, 293
MspR9I CCNGG 3 cut(s) 207, 239, 293
MunI CAATTG 2 cut(s) 345, 372
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 7 cut(s) 221, 230, 239, 326, 377, 391, 437
NciI CCSGG 2 cut(s) 207, 293
NdeII GATC 2 cut(s) 112, 508
NlaIII CATG 2 cut(s) 132, 450
NmuCI GTSAC 1 cut(s) 251
NspI RCATGY 2 cut(s) 132, 450
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 2 cut(s) 366, 430
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspPI GGNCC 1 cut(s) 214
RsaI GTAC 2 cut(s) 300, 338
RsaNI GTAC 2 cut(s) 299, 337
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 2 cut(s) 365, 429
Sau3AI GATC 2 cut(s) 112, 508
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 3 cut(s) 207, 239, 293
SduI GDGCHC 1 cut(s) 396
SetI ASST 5 cut(s) 11, 51, 191, 336, 382
SfaNI GCATC 2 cut(s) 233, 415
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 3 cut(s) 80, 345, 372
SsiI CCGC 2 cut(s) 68, 365
StyD4I CCNGG 3 cut(s) 205, 237, 291
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 51
TasI AATT 3 cut(s) 80, 345, 372
TatI WGTACW 2 cut(s) 298, 336
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 3 cut(s) 115, 264, 490
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 251
TspRI CASTG 3 cut(s) 115, 264, 490
XagI CCTNNNNNAGG 1 cut(s) 365
XceI RCATGY 2 cut(s) 132, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.