Rh4CG282500

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014918
Physical Location & Seq
Reverse (-)
54001418 .. 54002059
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG282500.1

Sequence Viewer

Length: 642 bp
ATGGAGTTTGTATATATATGTAAAGTTCATCGGCCACCAGAATCAGAAAAACCAAATCACACCCAATTCAACTTCTCCAATTTTCTTCCTTCTTCCCAATCTTCGACTCCCCAGAAAATGAACTACTCAATCCAAACCCCCCTTCTTCTTCTTATTGTTCATATTGTTTTCTTCAATGTGCAAACCCAATCCCAAAGCACTGATCTAATATCCAAAACATGCAAGAAAACACCCCACTTTGACCTTTGTCTCTCGTCTCTCCAATCAAGCCCTCAGAGCTCAAACTCAGACATTTCAGGCTTGGCTCACATAATGGCTGATCTTGTTCTGTCCAATGCAACTGATACGCTGGATTACATCCATGGCCTACTCAAGCAGTCCCCAGAAGCAGATTTGCAGAAGCCTTTGGCAAACTGTGCAGAGTTGTACATTCCAGTGGTGAAGTACACTCTCCCACAGTCAATTGATGCTCTCAGTAATGGGCATTATGGGTTTGCCAACTACGGCATTTCTGATGCTGCAAAGGAAGCTGAGGCTTGTGAGAAGGGGTTTTCCGGGGAGTCGCCGTTGACAGATAGGAACACTCTTGTGAGCAGCCTCGCTGAAGTAGCTGTGGCTATCATCAAGTTAGTGCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

213

Amino Acids

23.29

Weight (kDa)

5.57

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 207 6.8e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 624
AfaI GTAC 2 cut(s) 428, 446
AgsI TTSAA 2 cut(s) 70, 175
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AleI CACNNNNGTG 1 cut(s) 587
AluBI AGCT 3 cut(s) 279, 530, 611
AluI AGCT 3 cut(s) 279, 530, 611
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 2 cut(s) 254, 261
AoxI GGCC 2 cut(s) 32, 364
ApeKI GCWGC 2 cut(s) 518, 594
AsuC2I CCSGG 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 451
BanII GRGCYC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 531
BbvI GCAGC 2 cut(s) 505, 606
BceAI ACGGC 2 cut(s) 520, 550
BcnI CCSGG 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 254, 261
BisI GCNGC 2 cut(s) 519, 595
BlsI GCNGC 2 cut(s) 520, 596
Bme1390I CCNGG 1 cut(s) 556
BmrFI CCNGG 1 cut(s) 556
BmsI GCATC 2 cut(s) 457, 505
BoxI GACNNNNGTC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 556
BsaJI CCNNGG 2 cut(s) 361, 555
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bse1I ACTGG 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 361, 555
BseGI GGATG 1 cut(s) 357
BseMII CTCAG 4 cut(s) 287, 300, 487, 522
BseNI ACTGG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 505, 606
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 2 cut(s) 34, 366
BsiHKAI GWGCWC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 555
BslFI GGGAC 1 cut(s) 364
BsmAI GTCTC 2 cut(s) 254, 261
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 2 cut(s) 34, 366
Bsp1286I GDGCHC 1 cut(s) 281
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 2 cut(s) 202, 319
Bsp19I CCATGG 1 cut(s) 361
BspANI GGCC 2 cut(s) 34, 366
BspCNI CTCAG 4 cut(s) 286, 299, 486, 523
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 361, 555
BssMI GATC 2 cut(s) 202, 319
BssT1I CCWWGG 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 416, 459
BstAPI GCANNNNNTGC 1 cut(s) 416
BstAUI TGTACA 1 cut(s) 426
BstDEI CTNAG 4 cut(s) 273, 286, 473, 531
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 2 cut(s) 205, 322
BstMAI GTCTC 2 cut(s) 254, 261
BstMBI GATC 2 cut(s) 202, 319
BstMWI GCNNNNNNNGC 4 cut(s) 276, 416, 527, 608
BstNSI RCATGY 1 cut(s) 222
BstPAI GACNNNNGTC 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 554
BstV1I GCAGC 2 cut(s) 505, 606
BsuRI GGCC 2 cut(s) 34, 366
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 198, 441
Csp6I GTAC 2 cut(s) 427, 445
CviAII CATG 2 cut(s) 219, 362
CviQI GTAC 2 cut(s) 427, 445
DdeI CTNAG 4 cut(s) 273, 286, 473, 531
DpnI GATC 2 cut(s) 204, 321
DpnII GATC 2 cut(s) 202, 319
EaeI YGGCCR 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 624
EcoICRI GAGCTC 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 2 cut(s) 222, 365
FaiI YATR 9 cut(s) 13, 15, 17, 19, 162, 220, 311, 363, 489
FaqI GGGAC 1 cut(s) 364
FatI CATG 2 cut(s) 218, 361
Fnu4HI GCNGC 2 cut(s) 519, 595
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 519, 595
GluI GCNGC 2 cut(s) 519, 595
HaeIII GGCC 2 cut(s) 34, 366
HapII CCGG 1 cut(s) 555
Hin1II CATG 2 cut(s) 222, 365
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 3 cut(s) 41, 106, 560
HpaII CCGG 1 cut(s) 555
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 2 cut(s) 447, 570
Hpy188I TCNGA 4 cut(s) 46, 276, 289, 514
Hpy8I GTNNAC 2 cut(s) 447, 570
HpyAV CCTTC 3 cut(s) 99, 152, 538
HpyCH4III ACNGT 2 cut(s) 416, 459
HpyCH4V TGCA 7 cut(s) 181, 222, 338, 397, 419, 521, 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 276, 416, 527, 608
HpyF3I CTNAG 4 cut(s) 273, 286, 473, 531
Hsp92II CATG 2 cut(s) 222, 365
Kzo9I GATC 2 cut(s) 202, 319
LpnPI CCDG 8 cut(s) 51, 125, 282, 335, 396, 447, 568, 620
Lsp1109I GCAGC 2 cut(s) 505, 606
LweI GCATC 2 cut(s) 457, 505
MalI GATC 2 cut(s) 204, 321
MboI GATC 2 cut(s) 202, 319
MboII GAAGA 6 cut(s) 77, 84, 93, 137, 140, 163
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 3 cut(s) 65, 79, 462
MlyI GAGTC 2 cut(s) 100, 569
MnlI CCTC 3 cut(s) 282, 526, 608
MslI CAYNNNNRTG 2 cut(s) 434, 587
MspI CCGG 1 cut(s) 555
MspR9I CCNGG 1 cut(s) 556
MunI CAATTG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 276, 416, 527, 608
NciI CCSGG 1 cut(s) 556
NcoI CCATGG 1 cut(s) 361
NdeII GATC 2 cut(s) 202, 319
NlaIII CATG 2 cut(s) 222, 365
NspI RCATGY 1 cut(s) 222
OliI CACNNNNGTG 1 cut(s) 587
PfeI GAWTC 1 cut(s) 41
PkrI GCNGC 2 cut(s) 520, 596
PleI GAGTC 2 cut(s) 100, 568
PpsI GAGTC 2 cut(s) 100, 568
PshAI GACNNNNGTC 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 281
RsaI GTAC 2 cut(s) 428, 446
RsaNI GTAC 2 cut(s) 427, 445
RseI CAYNNNNRTG 2 cut(s) 434, 587
SacI GAGCTC 1 cut(s) 281
SatI GCNGC 2 cut(s) 519, 595
Sau3AI GATC 2 cut(s) 202, 319
SchI GAGTC 2 cut(s) 100, 569
ScrFI CCNGG 1 cut(s) 556
SduI GDGCHC 1 cut(s) 281
SetI ASST 4 cut(s) 246, 281, 532, 613
SfaNI GCATC 2 cut(s) 457, 505
SmiMI CAYNNNNRTG 2 cut(s) 434, 587
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 3 cut(s) 65, 79, 462
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 554
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 2 cut(s) 416, 459
TaqI TCGA 1 cut(s) 104
TasI AATT 3 cut(s) 65, 79, 462
TatI WGTACW 2 cut(s) 426, 444
TfiI GAWTC 1 cut(s) 41
TscAI CASTG 2 cut(s) 205, 441
TseI GCWGC 2 cut(s) 518, 594
TspDTI ATGAA 3 cut(s) 17, 134, 149
TspRI CASTG 2 cut(s) 205, 441
XceI RCATGY 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.