Rroxscaffold_5G00367840

Cell wall vacuolar inhibitor of fructosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014918
Physical Location & Seq
Reverse (-)
49692547 .. 49693070
524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367840.1

Sequence Viewer

Length: 420 bp
ATGAACTACTCAATCCAAACCCCCTTCTTCTTCTTCTTGTTCATATTCCCTGAGAGCTCAAACTCAGACATTTCAGGCTTGGCTCACATAATGGCTGATCTTGTTCTGTCCAATGCAACTGATACACTGGATTACATCCATGGCCTACTCAAGCAATCCCCAGAAGCAGATTTGCAGAAGCCTTTGGCAAACTGTGCAGAGTTGTACATTCCAGTGGTGAAGTACACTCTCCCACAGTCAATTGATGCTCTCAGCAATGGTCATTATGGGTTTGCCAACTACGGCATTTCTGATGCCGCAAAGGAAGCTGAGGCTTGTGAGAAGGGGTTTTCCGGGGAGTCGCCGTTGACAGATAGGAACACTCTTGTGAGCAGCCTCGCTGAAGTAGCTGTGGCTATCATCAAGTTGGTGCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

139

Amino Acids

15.04

Weight (kDa)

4.48

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 19 - 133 7.7e-15 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17130
fragaria_vesca FvH4_4g20180
malus_domestica MD13G1149800.v1.1 MD16G1150200.v1.1
prunus_persica Prupe.1G113800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0425861
rosa_laevigata RLG00000007334
rosa_multiflora Rmu_co8423965.1_g000001 Rmu_sc0002091.1_g000032 Rmu_ssc0000281.1_g000037
rosa_roxburghii Rroxscaffold_5G00367840
rosa_rugosa Rorug04G0207000
rosa_samantha Rh4AG264100 Rh4CG282500 Rh4DG266300
rosa_wichuraiana Rw4G022790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 206, 224
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 3 cut(s) 57, 308, 389
AluI AGCT 3 cut(s) 57, 308, 389
Alw21I GWGCWC 1 cut(s) 59
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 372
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 1 cut(s) 229
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 59
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 384
BceAI ACGGC 2 cut(s) 298, 328
BcnI CCSGG 1 cut(s) 334
BisI GCNGC 2 cut(s) 297, 373
BlsI GCNGC 2 cut(s) 298, 374
Bme1390I CCNGG 1 cut(s) 334
BmrFI CCNGG 1 cut(s) 334
BmsI GCATC 2 cut(s) 235, 283
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 334
BsaJI CCNNGG 2 cut(s) 139, 333
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse1I ACTGG 2 cut(s) 132, 212
Bse3DI GCAATG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 139, 333
BseGI GGATG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 4 cut(s) 42, 78, 265, 300
BseNI ACTGG 2 cut(s) 132, 212
BseXI GCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 216
BshFI GGCC 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 333
BsnI GGCC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 139
BspACI CCGC 1 cut(s) 297
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 4 cut(s) 43, 77, 264, 301
BsrDI GCAATG 1 cut(s) 262
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 132, 212
BssECI CCNNGG 2 cut(s) 139, 333
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 194, 237
BstAPI GCANNNNNTGC 1 cut(s) 194
BstAUI TGTACA 1 cut(s) 204
BstDEI CTNAG 4 cut(s) 51, 64, 251, 309
BstDSI CCRYGG 1 cut(s) 139
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 1 cut(s) 100
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 3 cut(s) 194, 305, 386
BstSCI CCNGG 1 cut(s) 332
BstV1I GCAGC 1 cut(s) 384
BsuRI GGCC 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 139
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 125, 219
Csp6I GTAC 2 cut(s) 205, 223
CviAII CATG 1 cut(s) 140
CviQI GTAC 2 cut(s) 205, 223
DdeI CTNAG 4 cut(s) 51, 64, 251, 309
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 143
FaiI YATR 4 cut(s) 44, 89, 141, 267
FatI CATG 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 297, 373
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 297, 373
GluI GCNGC 2 cut(s) 297, 373
HaeIII GGCC 1 cut(s) 144
HapII CCGG 1 cut(s) 333
Hin1II CATG 1 cut(s) 143
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 1 cut(s) 338
HpaII CCGG 1 cut(s) 333
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 225, 348
Hpy188I TCNGA 2 cut(s) 67, 292
Hpy8I GTNNAC 2 cut(s) 225, 348
HpyAV CCTTC 2 cut(s) 34, 316
HpyCH4III ACNGT 2 cut(s) 194, 237
HpyCH4V TGCA 4 cut(s) 116, 175, 197, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 305, 386
HpyF3I CTNAG 4 cut(s) 51, 64, 251, 309
Hsp92II CATG 1 cut(s) 143
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 7 cut(s) 60, 63, 113, 174, 225, 346, 398
Lsp1109I GCAGC 1 cut(s) 384
LweI GCATC 2 cut(s) 235, 283
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 3 cut(s) 19, 22, 25
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 1 cut(s) 240
MlyI GAGTC 1 cut(s) 347
MnlI CCTC 2 cut(s) 304, 386
MslI CAYNNNNRTG 2 cut(s) 212, 365
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 334
MunI CAATTG 1 cut(s) 240
MwoI GCNNNNNNNGC 3 cut(s) 194, 305, 386
NciI CCSGG 1 cut(s) 334
NcoI CCATGG 1 cut(s) 139
NdeII GATC 1 cut(s) 97
NlaIII CATG 1 cut(s) 143
OliI CACNNNNGTG 1 cut(s) 365
PkrI GCNGC 2 cut(s) 298, 374
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Psp124BI GAGCTC 1 cut(s) 59
RsaI GTAC 2 cut(s) 206, 224
RsaNI GTAC 2 cut(s) 205, 223
RseI CAYNNNNRTG 2 cut(s) 212, 365
SacI GAGCTC 1 cut(s) 59
SatI GCNGC 2 cut(s) 297, 373
Sau3AI GATC 1 cut(s) 97
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 334
SduI GDGCHC 1 cut(s) 59
SetI ASST 3 cut(s) 59, 310, 391
SfaNI GCATC 2 cut(s) 235, 283
SmiMI CAYNNNNRTG 2 cut(s) 212, 365
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 1 cut(s) 240
SsiI CCGC 1 cut(s) 297
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 332
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 194, 237
TasI AATT 1 cut(s) 240
TatI WGTACW 2 cut(s) 204, 222
TauI GCSGC 1 cut(s) 299
TscAI CASTG 2 cut(s) 132, 219
TseI GCWGC 1 cut(s) 372
TspDTI ATGAA 2 cut(s) 17, 31
TspRI CASTG 2 cut(s) 132, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.