AT3G17365

Thiopurine S-methyltransferase (TPMT)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012435
Physical Location & Seq
Reverse (-)
5946915 .. 5948868
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G17365.1

Sequence Viewer

Length: 720 bp
ATGGCTACAGAGGCACCAACGCAATCTTACAGCGAGCAATGGTATTGGGACGATCGTTACAAGAATGAATCTGAACCGTTTGATTGGTACCAGAAGTATTCACCTTTAGCTCCTCTCATCAATCTCTATGTTCCTCAACGTAATCAACGAGTCCTCGTCATTGGCTGTGGAAACTCAGCGTTTAGCGAAGGAATGGTGGATGATGGATACGAAGATGTAGTCAGTATTGATATATCATCAGTGGTGATTGATACCATGATTAAGAAATACTCTGATCGTCCTCAGCTCAAATATTTGAAGATGGATGTGCGTGATATGAAAGCATTTGAAGATGCTTCTTTTGATGCTGTTATCGATAAAGGAACCTTAGACTCCATTTTGTGTGGGAGTAATTCGAGGCAATACTCAACACAAATGCTTGAAGAGGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATCCTGATTACATATGGTGCTCCGATTTACCGTCTTCGCCTGTTTAAAGAATCATGCTCTTGGACAACCAAACTCCATGTTATAGACAAAAGTTTAACCGATCAACCGTTAGACACCCCAAAATGGGAACTGACAAAACCTCTTCCTCTAGATGCTGATGGAAGTTCAGTGGAATCTGCGATAGGCAAAAACCCTGATGTCCATTACATCTACGTCTGTATTAAGGATGAGTCTTCGAAGATGGAGGCAGATGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

239

Amino Acids

27.35

Weight (kDa)

4.72

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 15 - 157 2.7e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 47 - 177 5.7e-12 Methyltransferase domain
Methyltransf_25 PF13649 51 - 151 3.1e-11 Methyltransferase domain
Methyltransf_12 PF08242 52 - 152 1.3e-06 Methyltransferase domain
Methyltransf_11 PF08241 53 - 154 3.4e-14 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 13, 87
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 582, 583
AgsI TTSAA 3 cut(s) 298, 329, 422
AluBI AGCT 2 cut(s) 110, 286
AluI AGCT 2 cut(s) 110, 286
Alw21I GWGCWC 1 cut(s) 481
ApeKI GCWGC 1 cut(s) 713
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
Asp718I GGTACC 1 cut(s) 87
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 93, 256
AsuII TTCGAA 1 cut(s) 695
AvaII GGWCC 1 cut(s) 436
BanI GGYRCC 2 cut(s) 13, 87
BbsI GAAGAC 2 cut(s) 485, 684
Bbv12I GWGCWC 1 cut(s) 481
BbvCI CCTCAGC 1 cut(s) 282
BccI CCATC 4 cut(s) 197, 295, 611, 694
BciVI GTATCC 1 cut(s) 200
BfaI CTAG 1 cut(s) 608
BfmI CTRYAG 1 cut(s) 6
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 1 cut(s) 714
BlsI GCNGC 1 cut(s) 715
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 4 cut(s) 15, 89, 364, 437
BmsI GCATC 4 cut(s) 322, 334, 601, 700
BpiI GAAGAC 2 cut(s) 485, 684
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 582, 583
Bse3DI GCAATG 1 cut(s) 44
BseCI ATCGAT 1 cut(s) 354
BseGI GGATG 3 cut(s) 205, 310, 691
BseLI CCNNNNNNNGG 2 cut(s) 582, 583
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 189, 296
BseRI GAGGAG 1 cut(s) 102
Bsh1285I CGRYCG 1 cut(s) 55
BshNI GGYRCC 2 cut(s) 13, 87
BshVI ATCGAT 1 cut(s) 354
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 481
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 582, 583
BsmFI GGGAC 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 3 cut(s) 52, 274, 559
BspCNI CTCAG 2 cut(s) 188, 295
BspDI ATCGAT 1 cut(s) 354
BspLI GGNNCC 4 cut(s) 15, 89, 364, 437
BspT104I TTCGAA 1 cut(s) 695
BspT107I GGYRCC 2 cut(s) 13, 87
BsrDI GCAATG 1 cut(s) 44
BssMI GATC 3 cut(s) 52, 274, 559
Bst4CI ACNGT 3 cut(s) 78, 491, 567
Bst6I CTCTTC 2 cut(s) 417, 606
BstBI TTCGAA 1 cut(s) 695
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 3 cut(s) 175, 282, 367
BstF5I GGATG 3 cut(s) 205, 310, 691
BstKTI GATC 3 cut(s) 55, 277, 562
BstMBI GATC 3 cut(s) 52, 274, 559
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 2 cut(s) 11, 713
BstSFI CTRYAG 1 cut(s) 6
BstV2I GAAGAC 2 cut(s) 485, 684
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 354
BtsCI GGATG 3 cut(s) 205, 310, 691
BtsIMutI CAGTG 2 cut(s) 246, 633
Cac8I GCNNGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 1 cut(s) 88
CviAII CATG 4 cut(s) 256, 513, 536, 717
CviJI RGCY 4 cut(s) 5, 110, 165, 286
CviKI_1 RGCY 4 cut(s) 5, 110, 165, 286
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 3 cut(s) 175, 282, 367
DpnI GATC 3 cut(s) 54, 276, 561
DpnII GATC 3 cut(s) 52, 274, 559
DraI TTTAAA 1 cut(s) 505
Eam1104I CTCTTC 2 cut(s) 417, 606
EarI CTCTTC 2 cut(s) 417, 606
Eco47I GGWCC 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 436
FaeI CATG 4 cut(s) 259, 516, 539, 720
FaqI GGGAC 1 cut(s) 62
FatI CATG 4 cut(s) 255, 512, 535, 716
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 714
FokI GGATG 3 cut(s) 212, 317, 698
Fsp4HI GCNGC 1 cut(s) 714
FspBI CTAG 1 cut(s) 608
GluI GCNGC 1 cut(s) 714
Hin1II CATG 4 cut(s) 259, 516, 539, 720
HinfI GANTC 7 cut(s) 68, 150, 371, 453, 509, 632, 689
HphI GGTGA 2 cut(s) 93, 256
Hpy188I TCNGA 3 cut(s) 73, 274, 483
Hpy188III TCNNGA 2 cut(s) 463, 608
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 3 cut(s) 78, 491, 567
HpyCH4IV ACGT 2 cut(s) 139, 672
HpyCH4V TGCA 1 cut(s) 713
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 713
HpyF3I CTNAG 3 cut(s) 175, 282, 367
HpySE526I ACGT 2 cut(s) 139, 672
Hsp92II CATG 4 cut(s) 259, 516, 539, 720
KpnI GGTACC 1 cut(s) 91
Kzo9I GATC 3 cut(s) 52, 274, 559
LmnI GCTCC 2 cut(s) 115, 484
LpnPI CCDG 4 cut(s) 104, 476, 512, 666
LweI GCATC 4 cut(s) 322, 334, 601, 700
MaeI CTAG 1 cut(s) 608
MaeII ACGT 2 cut(s) 139, 672
MaeIII GTNAC 1 cut(s) 56
MalI GATC 3 cut(s) 54, 276, 561
MboI GATC 3 cut(s) 52, 274, 559
MboII GAAGA 8 cut(s) 224, 310, 341, 434, 485, 593, 684, 709
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 1 cut(s) 391
MlyI GAGTC 4 cut(s) 159, 365, 462, 698
MseI TTAA 4 cut(s) 261, 504, 554, 681
MwoI GCNNNNNNNGC 2 cut(s) 11, 713
NdeI CATATG 1 cut(s) 472
NdeII GATC 3 cut(s) 52, 274, 559
NlaIII CATG 4 cut(s) 259, 516, 539, 720
NlaIV GGNNCC 4 cut(s) 15, 89, 364, 437
NspV TTCGAA 1 cut(s) 695
PcsI WCGNNNNNNNCGW 1 cut(s) 145
PfeI GAWTC 3 cut(s) 68, 509, 632
PkrI GCNGC 1 cut(s) 715
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 4 cut(s) 158, 365, 461, 697
PpsI GAGTC 4 cut(s) 158, 365, 461, 697
PpuMI RGGWCCY 1 cut(s) 436
Psp5II RGGWCCY 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 15, 89, 364, 437
PspPI GGNCC 1 cut(s) 436
PspPPI RGGWCCY 1 cut(s) 436
PvuI CGATCG 1 cut(s) 55
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 261, 504, 554, 681
SatI GCNGC 1 cut(s) 714
Sau3AI GATC 3 cut(s) 52, 274, 559
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 4 cut(s) 159, 365, 462, 698
SduI GDGCHC 1 cut(s) 481
SetI ASST 8 cut(s) 106, 112, 142, 288, 368, 429, 601, 675
SfaNI GCATC 4 cut(s) 322, 334, 601, 700
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 695
SinI GGWCC 1 cut(s) 436
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 1 cut(s) 391
SspI AATATT 1 cut(s) 293
SspMI CTAG 1 cut(s) 608
TaaI ACNGT 3 cut(s) 78, 491, 567
TaiI ACGT 2 cut(s) 142, 675
TaqI TCGA 3 cut(s) 354, 395, 695
TasI AATT 1 cut(s) 391
TfiI GAWTC 3 cut(s) 68, 509, 632
Tru1I TTAA 4 cut(s) 261, 504, 554, 681
Tru9I TTAA 4 cut(s) 261, 504, 554, 681
TscAI CASTG 2 cut(s) 246, 633
TseI GCWGC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 81, 332
TspRI CASTG 2 cut(s) 246, 633
VpaK11BI GGWCC 1 cut(s) 436
XbaI TCTAGA 1 cut(s) 607
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.