RchiOBHm_Chr4g0426751

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012435
Physical Location & Seq
Reverse (-)
52160816 .. 52163704
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39573

Sequence Viewer

Length: 858 bp
ATGACGGAACGGCCCACACATGCTTGTGGTCGTAAACAGAAAGCGAACGCCATCACAGGAACCGACAAAACCAACCGATTACTGAAGAGTGAGATGACGCTGGGAGGAGCCACGACGACGACGACGACGACGCAGCAGCAAGCCTACGGCGAGCCATGGTACTGGGACAACCGCTACGCCAACGAATCCGGGGCGTTCGATTGGTACCAGAAGTACCAATCTCTAGCTCCCATTATCAATCTGTACGTCCCCCGCCACAACCACCTCCGCCACCGCATCCTCGTCGTCGGCTGCGGCAACTCCGCATTTAGCGAAGGAATGGCGGACGATGGGTATGAGGAGGTGCTTAGTATCGACATTTCATCTGTGGTCATCCAAGCTATGCAGGAGAAGCACTCCAATCGTCAAGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCCAAACCGCTTCCTTTGATGCTGTTATTGACAAAGGAACTCTAGACTCTCTTCTGTGCGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAGTGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTCACATATGGAGCCCCGTTGTATCGTTTACGGTTGCTGAAAGAATCAAGCTCGTGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCGATGATAAATCAGAACATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGAAGCTCAGCAGAGGAATTGCTAGGAAACAACCCTGATGTCCATTTTATTTACATTTGTACGAAGGATAATTCTTTGAAGCCAGGCCTTAAGCGTGAAGTGTCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

285

Amino Acids

32.15

Weight (kDa)

5.78

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 54 - 199 1.2e-06 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 92 - 201 2.1e-14 Methyltransferase domain
Methyltransf_25 PF13649 93 - 193 6.7e-12 Methyltransferase domain
Methyltransf_11 PF08241 94 - 196 7.8e-15 Methyltransferase domain
Methyltransf_12 PF08242 94 - 194 2.5e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 852
AciI CCGC 9 cut(s) 172, 253, 268, 274, 294, 303, 323, 459, 510
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 5 cut(s) 161, 206, 215, 245, 805
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 2 cut(s) 736, 823
AjnI CCWGG 1 cut(s) 826
AluBI AGCT 5 cut(s) 227, 380, 642, 715, 750
AluI AGCT 5 cut(s) 227, 380, 642, 715, 750
AoxI GGCC 2 cut(s) 11, 829
ApeKI GCWGC 4 cut(s) 133, 136, 291, 533
Asp700I GAANNNNTTC 2 cut(s) 449, 517
Asp718I GGTACC 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 12, 562
AsuC2I CCSGG 1 cut(s) 190
AvaII GGWCC 1 cut(s) 562
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 607
BauI CACGAG 1 cut(s) 643
BbvI GCAGC 4 cut(s) 145, 148, 278, 520
BccI CCATC 3 cut(s) 59, 323, 737
BceAI ACGGC 2 cut(s) 26, 163
BcgI CGANNNNNNTGC 4 cut(s) 265, 299, 383, 417
BciT130I CCWGG 1 cut(s) 828
BcnI CCSGG 1 cut(s) 190
BfaI CTAG 3 cut(s) 224, 494, 767
BfrI CTTAAG 1 cut(s) 833
BisI GCNGC 5 cut(s) 134, 137, 292, 295, 534
BlpI GCTNAGC 1 cut(s) 751
BlsI GCNGC 5 cut(s) 135, 138, 293, 296, 535
Bme1390I CCNGG 2 cut(s) 190, 828
Bme18I GGWCC 1 cut(s) 562
BmgT120I GGNCC 2 cut(s) 12, 562
BmiI GGNNCC 5 cut(s) 61, 109, 206, 563, 604
BmrFI CCNGG 2 cut(s) 190, 828
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 3 cut(s) 285, 460, 531
BmuI ACTGGG 1 cut(s) 172
BplI GAGNNNNNCTC 2 cut(s) 380, 412
Bpu1102I GCTNAGC 1 cut(s) 751
BpuEI CTTGAG 1 cut(s) 551
BpuMI CCSGG 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 155, 189
Bse1I ACTGG 1 cut(s) 167
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 2 cut(s) 155, 189
BseGI GGATG 5 cut(s) 276, 372, 436, 546, 700
BseMII CTCAG 2 cut(s) 559, 765
BseNI ACTGG 1 cut(s) 167
BseRI GAGGAG 2 cut(s) 120, 353
BseXI GCAGC 4 cut(s) 145, 148, 278, 520
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 2 cut(s) 13, 831
BshNI GGYRCC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 2 cut(s) 179, 233
BsmFI GGGAC 2 cut(s) 179, 233
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 2 cut(s) 13, 831
Bsp1286I GDGCHC 1 cut(s) 607
Bsp1720I GCTNAGC 1 cut(s) 751
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 9 cut(s) 172, 253, 268, 274, 294, 303, 323, 459, 510
BspANI GGCC 2 cut(s) 13, 831
BspCNI CTCAG 2 cut(s) 558, 764
BspLI GGNNCC 5 cut(s) 61, 109, 206, 563, 604
BspT107I GGYRCC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 833
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 155, 189
BssSI CACGAG 1 cut(s) 643
BssT1I CCWWGG 1 cut(s) 155
Bst2BI CACGAG 1 cut(s) 643
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 1 cut(s) 624
Bst6I CTCTTC 2 cut(s) 80, 507
BstAFI CTTAAG 1 cut(s) 833
BstC8I GCNNGC 2 cut(s) 141, 152
BstDEI CTNAG 3 cut(s) 347, 545, 751
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 5 cut(s) 276, 372, 436, 546, 700
BstMWI GCNNNNNNNGC 1 cut(s) 391
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 188, 826
BstV1I GCAGC 4 cut(s) 145, 148, 278, 520
BstXI CCANNNNNNTGG 1 cut(s) 162
BsuRI GGCC 2 cut(s) 13, 831
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 5 cut(s) 276, 372, 436, 546, 700
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 141, 152
Cfr13I GGNCC 2 cut(s) 12, 562
CseI GACGC 2 cut(s) 106, 139
Csp6I GTAC 5 cut(s) 160, 205, 214, 244, 804
CviAII CATG 3 cut(s) 20, 156, 662
CviQI GTAC 5 cut(s) 160, 205, 214, 244, 804
DdeI CTNAG 3 cut(s) 347, 545, 751
Eam1104I CTCTTC 2 cut(s) 80, 507
EarI CTCTTC 2 cut(s) 80, 507
EciI GGCGGA 2 cut(s) 257, 338
Eco130I CCWWGG 1 cut(s) 155
Eco147I AGGCCT 1 cut(s) 831
Eco24I GRGCYC 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 3 cut(s) 23, 159, 665
FaqI GGGAC 2 cut(s) 179, 233
FatI CATG 3 cut(s) 19, 155, 661
FauI CCCGC 1 cut(s) 260
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 1 cut(s) 852
Fnu4HI GCNGC 5 cut(s) 134, 137, 292, 295, 534
FokI GGATG 5 cut(s) 263, 359, 443, 553, 687
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 5 cut(s) 134, 137, 292, 295, 534
FspBI CTAG 3 cut(s) 224, 494, 767
GluI GCNGC 5 cut(s) 134, 137, 292, 295, 534
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 13, 831
HapII CCGG 1 cut(s) 189
HgaI GACGC 2 cut(s) 106, 139
Hin1II CATG 3 cut(s) 23, 159, 665
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HinfI GANTC 4 cut(s) 185, 497, 579, 635
HpaII CCGG 1 cut(s) 189
Hpy166II GTNNAC 3 cut(s) 35, 620, 853
Hpy188I TCNGA 3 cut(s) 540, 685, 697
Hpy188III TCNNGA 2 cut(s) 407, 494
Hpy8I GTNNAC 3 cut(s) 35, 620, 853
Hpy99I CGWCG 8 cut(s) 118, 121, 124, 127, 130, 133, 287, 290
HpyAV CCTTC 2 cut(s) 308, 802
HpyCH4III ACNGT 1 cut(s) 624
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 391
HpyF3I CTNAG 3 cut(s) 347, 545, 751
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 3 cut(s) 23, 159, 665
KpnI GGTACC 1 cut(s) 208
LmnI GCTCC 4 cut(s) 107, 232, 602, 712
Lsp1109I GCAGC 4 cut(s) 145, 148, 278, 520
LweI GCATC 3 cut(s) 285, 460, 531
MaeI CTAG 3 cut(s) 224, 494, 767
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 592
MboII GAAGA 2 cut(s) 97, 494
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 3 cut(s) 517, 761, 814
MlyI GAGTC 2 cut(s) 491, 588
MnlI CCTC 8 cut(s) 98, 275, 290, 331, 334, 552, 575, 751
MroXI GAANNNNTTC 2 cut(s) 449, 517
MseI TTAA 2 cut(s) 414, 834
MslI CAYNNNNRTG 1 cut(s) 24
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 2 cut(s) 190, 828
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 1 cut(s) 828
MwoI GCNNNNNNNGC 1 cut(s) 391
NciI CCSGG 1 cut(s) 190
NcoI CCATGG 1 cut(s) 155
NdeI CATATG 1 cut(s) 598
NlaIII CATG 3 cut(s) 23, 159, 665
NlaIV GGNNCC 5 cut(s) 61, 109, 206, 563, 604
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 1 cut(s) 23
PceI AGGCCT 1 cut(s) 831
PcsI WCGNNNNNNNCGW 3 cut(s) 119, 122, 125
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 2 cut(s) 449, 517
PfeI GAWTC 2 cut(s) 185, 635
PkrI GCNGC 5 cut(s) 135, 138, 293, 296, 535
PleI GAGTC 2 cut(s) 491, 587
PpsI GAGTC 2 cut(s) 491, 587
PpuMI RGGWCCY 1 cut(s) 562
PsiI TTATAA 1 cut(s) 653
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 5 cut(s) 61, 109, 206, 563, 604
PspPI GGNCC 2 cut(s) 12, 562
PspPPI RGGWCCY 1 cut(s) 562
RsaI GTAC 5 cut(s) 161, 206, 215, 245, 805
RsaNI GTAC 5 cut(s) 160, 205, 214, 244, 804
RseI CAYNNNNRTG 1 cut(s) 24
SalI GTCGAC 1 cut(s) 851
SaqAI TTAA 2 cut(s) 414, 834
SatI GCNGC 5 cut(s) 134, 137, 292, 295, 534
Sau96I GGNCC 2 cut(s) 12, 562
SchI GAGTC 2 cut(s) 491, 588
ScrFI CCNGG 2 cut(s) 190, 828
SduI GDGCHC 1 cut(s) 607
SetI ASST 9 cut(s) 229, 249, 267, 345, 382, 414, 644, 717, 752
SfaNI GCATC 3 cut(s) 285, 460, 531
SinI GGWCC 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 2 cut(s) 566, 833
SmoI CTYRAG 2 cut(s) 566, 833
Sse9I AATT 3 cut(s) 517, 761, 814
SseBI AGGCCT 1 cut(s) 831
SsiI CCGC 9 cut(s) 172, 253, 268, 274, 294, 303, 323, 459, 510
SspI AATATT 1 cut(s) 419
SspMI CTAG 3 cut(s) 224, 494, 767
StuI AGGCCT 1 cut(s) 831
StyD4I CCNGG 2 cut(s) 188, 826
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 624
TaiI ACGT 1 cut(s) 249
TaqI TCGA 3 cut(s) 198, 354, 852
TasI AATT 3 cut(s) 517, 761, 814
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 2 cut(s) 185, 635
Tru1I TTAA 2 cut(s) 414, 834
Tru9I TTAA 2 cut(s) 414, 834
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 4 cut(s) 133, 136, 291, 533
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 2 cut(s) 351, 458
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 1 cut(s) 553
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 1 cut(s) 562
XbaI TCTAGA 1 cut(s) 493
XceI RCATGY 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 852
XmnI GAANNNNTTC 2 cut(s) 449, 517
XspI CTAG 3 cut(s) 224, 494, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.