Rh4DG273000

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012435
Physical Location & Seq
Reverse (-)
49286902 .. 49289626
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG273000.1

Sequence Viewer

Length: 768 bp
ATGACGCTGGGAGGAGCCACGACGACGACGACGACGACGACGCAGCAGCAAGCCTACGGCGAGCCATGGTACTGGGACAACCGCTACGCCAACGAATCCGGGGCGTTCGATTGGTACCAGAAGTACCAATCTCTAGCTCCCATTATCAATCTGTACGTCCCCCGCCACAACCACCTCCGCCACCGCATCCTCGTCGTCGGCTGCGGCAACTCCGCATTTAGCGAAGGAATGGCGGACGATGGGTACGAGGAGGTGGTCAGTATCGACATTTCATCTGTGGTCATCCAAGCTATGCAGGAGAAGCACTCCAATCGTCAAGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCCAAGCCGCTTCCTTTGATGCTGTTATTGACAAAGGAACTCTAGACTCTCTTCTGTGCGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAGTGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTCACATATGGAGCCCCGTTGTATCGTTTACGGTTGCTGAAAGAATCAAGCTCGTGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCGATGATAAATCAGAACATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGGAGCTCAGCAGAGGAATTGCTAGGAAACAACCCTGATGTTCATTTTATTTACATTTGTACGAAGGATAACTCTTTGAAGCCAGGCCTTAAGCGTGAAGTGTCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

255

Amino Acids

28.77

Weight (kDa)

5.16

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 24 - 169 3.7e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 62 - 171 4.4e-15 Methyltransferase domain
Methyltransf_25 PF13649 63 - 163 2.1e-12 Methyltransferase domain
Methyltransf_11 PF08241 64 - 166 2.4e-15 Methyltransferase domain
Methyltransf_12 PF08242 64 - 164 1.4e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
Acc65I GGTACC 1 cut(s) 114
AccB1I GGYRCC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 762
AciI CCGC 9 cut(s) 82, 163, 178, 184, 204, 213, 233, 369, 420
AfaI GTAC 6 cut(s) 71, 116, 125, 155, 245, 715
AflII CTTAAG 1 cut(s) 743
AgsI TTSAA 2 cut(s) 646, 733
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 5 cut(s) 137, 290, 552, 625, 660
AluI AGCT 5 cut(s) 137, 290, 552, 625, 660
Alw21I GWGCWC 1 cut(s) 662
AoxI GGCC 1 cut(s) 739
ApeKI GCWGC 4 cut(s) 43, 46, 201, 443
Asp700I GAANNNNTTC 2 cut(s) 359, 427
Asp718I GGTACC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 472
AsuC2I CCSGG 1 cut(s) 100
AvaII GGWCC 1 cut(s) 472
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 2 cut(s) 517, 662
BauI CACGAG 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 662
BbvI GCAGC 4 cut(s) 55, 58, 188, 430
BccI CCATC 2 cut(s) 233, 647
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 293, 327
BciT130I CCWGG 1 cut(s) 738
BcnI CCSGG 1 cut(s) 100
BfaI CTAG 3 cut(s) 134, 404, 677
BfrI CTTAAG 1 cut(s) 743
BisI GCNGC 6 cut(s) 44, 47, 202, 205, 369, 444
BlpI GCTNAGC 1 cut(s) 661
BlsI GCNGC 6 cut(s) 45, 48, 203, 206, 370, 445
Bme1390I CCNGG 2 cut(s) 100, 738
Bme18I GGWCC 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 4 cut(s) 16, 116, 473, 514
BmrFI CCNGG 2 cut(s) 100, 738
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 3 cut(s) 195, 370, 441
BmuI ACTGGG 1 cut(s) 82
BplI GAGNNNNNCTC 2 cut(s) 290, 322
Bpu1102I GCTNAGC 1 cut(s) 661
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 65, 99
Bse1I ACTGG 1 cut(s) 77
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 2 cut(s) 65, 99
BseGI GGATG 5 cut(s) 186, 282, 346, 456, 610
BseMII CTCAG 2 cut(s) 469, 675
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 27, 263
BseXI GCAGC 4 cut(s) 55, 58, 188, 430
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 741
BshNI GGYRCC 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 662
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 89, 143
BsmFI GGGAC 2 cut(s) 89, 143
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 741
Bsp1286I GDGCHC 2 cut(s) 517, 662
Bsp1720I GCTNAGC 1 cut(s) 661
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 9 cut(s) 82, 163, 178, 184, 204, 213, 233, 369, 420
BspANI GGCC 1 cut(s) 741
BspCNI CTCAG 2 cut(s) 468, 674
BspLI GGNNCC 4 cut(s) 16, 116, 473, 514
BspT107I GGYRCC 1 cut(s) 114
BspTI CTTAAG 1 cut(s) 743
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 65, 99
BssSI CACGAG 1 cut(s) 553
BssT1I CCWWGG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 553
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 1 cut(s) 534
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 743
BstC8I GCNNGC 2 cut(s) 51, 62
BstDEI CTNAG 2 cut(s) 455, 661
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 5 cut(s) 186, 282, 346, 456, 610
BstMWI GCNNNNNNNGC 2 cut(s) 301, 365
BstNI CCWGG 1 cut(s) 738
BstSCI CCNGG 2 cut(s) 98, 736
BstV1I GCAGC 4 cut(s) 55, 58, 188, 430
BstXI CCANNNNNNTGG 1 cut(s) 72
BsuRI GGCC 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 5 cut(s) 186, 282, 346, 456, 610
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 51, 62
Cfr13I GGNCC 1 cut(s) 472
CseI GACGC 2 cut(s) 13, 49
Csp6I GTAC 6 cut(s) 70, 115, 124, 154, 244, 714
CviAII CATG 2 cut(s) 66, 572
CviQI GTAC 6 cut(s) 70, 115, 124, 154, 244, 714
DdeI CTNAG 2 cut(s) 455, 661
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 2 cut(s) 167, 248
Ecl136II GAGCTC 1 cut(s) 660
Eco130I CCWWGG 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 2 cut(s) 517, 662
Eco47I GGWCC 1 cut(s) 472
Eco53kI GAGCTC 1 cut(s) 660
EcoICRI GAGCTC 1 cut(s) 660
EcoO109I RGGNCCY 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 65
EcoT38I GRGCYC 2 cut(s) 517, 662
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 2 cut(s) 69, 575
FaiI YATR 9 cut(s) 67, 293, 353, 495, 508, 510, 563, 573, 619
FaqI GGGAC 2 cut(s) 89, 143
FatI CATG 2 cut(s) 65, 571
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 508
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 6 cut(s) 44, 47, 202, 205, 369, 444
FokI GGATG 5 cut(s) 173, 269, 353, 463, 597
FriOI GRGCYC 2 cut(s) 517, 662
Fsp4HI GCNGC 6 cut(s) 44, 47, 202, 205, 369, 444
FspBI CTAG 3 cut(s) 134, 404, 677
GluI GCNGC 6 cut(s) 44, 47, 202, 205, 369, 444
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 741
HapII CCGG 1 cut(s) 99
HgaI GACGC 2 cut(s) 13, 49
Hin1II CATG 2 cut(s) 69, 575
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 4 cut(s) 95, 407, 489, 545
HpaII CCGG 1 cut(s) 99
Hpy166II GTNNAC 2 cut(s) 530, 763
Hpy188I TCNGA 3 cut(s) 450, 595, 607
Hpy188III TCNNGA 2 cut(s) 317, 404
Hpy8I GTNNAC 2 cut(s) 530, 763
Hpy99I CGWCG 9 cut(s) 25, 28, 31, 34, 37, 40, 43, 197, 200
HpyAV CCTTC 2 cut(s) 218, 712
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 365
HpyF3I CTNAG 2 cut(s) 455, 661
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 2 cut(s) 69, 575
KpnI GGTACC 1 cut(s) 118
LmnI GCTCC 5 cut(s) 14, 142, 512, 622, 657
LpnPI CCDG 9 cut(s) 58, 112, 131, 281, 485, 648, 702, 723, 750
Lsp1109I GCAGC 4 cut(s) 55, 58, 188, 430
LweI GCATC 3 cut(s) 195, 370, 441
MaeI CTAG 3 cut(s) 134, 404, 677
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 502
MboII GAAGA 1 cut(s) 404
MhlI GDGCHC 2 cut(s) 517, 662
MluCI AATT 2 cut(s) 427, 671
MlyI GAGTC 2 cut(s) 401, 498
MnlI CCTC 8 cut(s) 5, 185, 200, 241, 244, 462, 485, 661
MroXI GAANNNNTTC 2 cut(s) 359, 427
MseI TTAA 2 cut(s) 324, 744
MspCI CTTAAG 1 cut(s) 743
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 100, 738
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 738
MwoI GCNNNNNNNGC 2 cut(s) 301, 365
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 508
NlaIII CATG 2 cut(s) 69, 575
NlaIV GGNNCC 4 cut(s) 16, 116, 473, 514
NmuCI GTSAC 1 cut(s) 502
PceI AGGCCT 1 cut(s) 741
PcsI WCGNNNNNNNCGW 5 cut(s) 26, 29, 32, 35, 243
PctI GAATGC 1 cut(s) 361
PdmI GAANNNNTTC 2 cut(s) 359, 427
PfeI GAWTC 2 cut(s) 95, 545
PkrI GCNGC 6 cut(s) 45, 48, 203, 206, 370, 445
PleI GAGTC 2 cut(s) 401, 497
PpsI GAGTC 2 cut(s) 401, 497
PpuMI RGGWCCY 1 cut(s) 472
PsiI TTATAA 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 662
Psp5II RGGWCCY 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 736
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 4 cut(s) 16, 116, 473, 514
PspPI GGNCC 1 cut(s) 472
PspPPI RGGWCCY 1 cut(s) 472
RsaI GTAC 6 cut(s) 71, 116, 125, 155, 245, 715
RsaNI GTAC 6 cut(s) 70, 115, 124, 154, 244, 714
SacI GAGCTC 1 cut(s) 662
SalI GTCGAC 1 cut(s) 761
SaqAI TTAA 2 cut(s) 324, 744
SatI GCNGC 6 cut(s) 44, 47, 202, 205, 369, 444
Sau96I GGNCC 1 cut(s) 472
SchI GAGTC 2 cut(s) 401, 498
ScrFI CCNGG 2 cut(s) 100, 738
SduI GDGCHC 2 cut(s) 517, 662
SetI ASST 9 cut(s) 139, 159, 177, 255, 292, 324, 554, 627, 662
SfaNI GCATC 3 cut(s) 195, 370, 441
SinI GGWCC 1 cut(s) 472
SmlI CTYRAG 2 cut(s) 476, 743
SmoI CTYRAG 2 cut(s) 476, 743
Sse9I AATT 2 cut(s) 427, 671
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 9 cut(s) 82, 163, 178, 184, 204, 213, 233, 369, 420
SspI AATATT 1 cut(s) 329
SspMI CTAG 3 cut(s) 134, 404, 677
SstI GAGCTC 1 cut(s) 662
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 2 cut(s) 98, 736
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 534
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 108, 264, 762
TasI AATT 2 cut(s) 427, 671
TauI GCSGC 2 cut(s) 207, 371
TfiI GAWTC 2 cut(s) 95, 545
Tru1I TTAA 2 cut(s) 324, 744
Tru9I TTAA 2 cut(s) 324, 744
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 4 cut(s) 43, 46, 201, 443
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 3 cut(s) 261, 368, 686
TspRI CASTG 1 cut(s) 463
Vha464I CTTAAG 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 472
XbaI TCTAGA 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 2 cut(s) 359, 427
XspI CTAG 3 cut(s) 134, 404, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.