Rh4CG290600

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012435
Physical Location & Seq
Reverse (-)
54915874 .. 54918571
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG290600.1

Sequence Viewer

Length: 753 bp
ATGACGCTGGGAGGAGCCACGACGACGGCGACGACGACGACGCAGCAGCAAGCCTACGGCGAGCCATGGTACTGGGACAACCGCTACGCCAACGAATCCGGGGCGTTCGATTGGTACCAGAAGTACCAATCTCTAGCTCCCATTATCAATGTGTACGTCCCCCGCCACCGCATCCTCGTCGTGGGCTGCGGCAACTCCGCATTTAGCGAAGGAATGGCGGACGATGGGTACGAGGAGGTGGTTAGTATCGACATTTCATCTGTGGTCATCCAAGCTATGCAGGAGAAGCACTCCAATCGTCAAGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCCAAACCGCTTCCTTTGATGCTGTTATTGACAAAGGAACTCTAGACTCTCTTCTGTGCGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAGTGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTCACATATGGAGCCCCGTTGTATCGTTTACGGTTGCTGAAAGAATCAAGCTCGTGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCGATGATAAATCAGAACATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGAAGCTCAGCAGAGGAATTGCTAGGAAACAACCCTGATGTCCATTTTATTTACATTTGTACGAAGGATAATTCTTTGAAGCCAGGCCTTAAGCGTGAAGTGTCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

250

Amino Acids

28.09

Weight (kDa)

4.9

Isoelectric Point (pI)

24.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 24 - 164 9e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 57 - 166 3.3e-15 Methyltransferase domain
Methyltransf_25 PF13649 58 - 158 2.1e-12 Methyltransferase domain
Methyltransf_11 PF08241 59 - 161 2.6e-15 Methyltransferase domain
Methyltransf_12 PF08242 59 - 159 9.9e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 114
AccB1I GGYRCC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 747
AciI CCGC 8 cut(s) 82, 163, 169, 189, 198, 218, 354, 405
AfaI GTAC 6 cut(s) 71, 116, 125, 155, 230, 700
AfiI CCNNNNNNNGG 1 cut(s) 181
AflII CTTAAG 1 cut(s) 728
AgsI TTSAA 2 cut(s) 631, 718
AjnI CCWGG 1 cut(s) 721
AluBI AGCT 5 cut(s) 137, 275, 537, 610, 645
AluI AGCT 5 cut(s) 137, 275, 537, 610, 645
AoxI GGCC 1 cut(s) 724
ApeKI GCWGC 4 cut(s) 43, 46, 186, 428
Asp700I GAANNNNTTC 2 cut(s) 344, 412
Asp718I GGTACC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 457
AsuC2I CCSGG 1 cut(s) 100
AvaII GGWCC 1 cut(s) 457
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 502
BauI CACGAG 1 cut(s) 538
BbvI GCAGC 4 cut(s) 55, 58, 173, 415
BccI CCATC 2 cut(s) 218, 632
BceAI ACGGC 2 cut(s) 42, 73
BcgI CGANNNNNNTGC 4 cut(s) 160, 194, 278, 312
BciT130I CCWGG 1 cut(s) 723
BcnI CCSGG 1 cut(s) 100
BfaI CTAG 3 cut(s) 134, 389, 662
BfrI CTTAAG 1 cut(s) 728
BisI GCNGC 5 cut(s) 44, 47, 187, 190, 429
BlpI GCTNAGC 1 cut(s) 646
BlsI GCNGC 5 cut(s) 45, 48, 188, 191, 430
Bme1390I CCNGG 2 cut(s) 100, 723
Bme18I GGWCC 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 4 cut(s) 16, 116, 458, 499
BmrFI CCNGG 2 cut(s) 100, 723
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 3 cut(s) 180, 355, 426
BmuI ACTGGG 1 cut(s) 82
BplI GAGNNNNNCTC 2 cut(s) 275, 307
Bpu1102I GCTNAGC 1 cut(s) 646
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 65, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 77
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 65, 99
BseGI GGATG 5 cut(s) 171, 267, 331, 441, 595
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 454, 660
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 27, 248
BseXI GCAGC 4 cut(s) 55, 58, 173, 415
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 726
BshNI GGYRCC 1 cut(s) 114
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 89, 143
BslI CCNNNNNNNGG 1 cut(s) 181
BsmFI GGGAC 2 cut(s) 89, 143
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1720I GCTNAGC 1 cut(s) 646
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 8 cut(s) 82, 163, 169, 189, 198, 218, 354, 405
BspANI GGCC 1 cut(s) 726
BspCNI CTCAG 2 cut(s) 453, 659
BspLI GGNNCC 4 cut(s) 16, 116, 458, 499
BspT107I GGYRCC 1 cut(s) 114
BspTI CTTAAG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 65, 99
BssSI CACGAG 1 cut(s) 538
BssT1I CCWWGG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 538
Bst2UI CCWGG 1 cut(s) 723
Bst4CI ACNGT 1 cut(s) 519
Bst6I CTCTTC 1 cut(s) 402
BstAFI CTTAAG 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 51, 62
BstDEI CTNAG 2 cut(s) 440, 646
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 5 cut(s) 171, 267, 331, 441, 595
BstMWI GCNNNNNNNGC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 723
BstSCI CCNGG 2 cut(s) 98, 721
BstV1I GCAGC 4 cut(s) 55, 58, 173, 415
BstXI CCANNNNNNTGG 1 cut(s) 72
BsuRI GGCC 1 cut(s) 726
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 5 cut(s) 171, 267, 331, 441, 595
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 2 cut(s) 51, 62
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 2 cut(s) 13, 49
Csp6I GTAC 6 cut(s) 70, 115, 124, 154, 229, 699
CviAII CATG 2 cut(s) 66, 557
CviQI GTAC 6 cut(s) 70, 115, 124, 154, 229, 699
DdeI CTNAG 2 cut(s) 440, 646
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 457
EcoO109I RGGNCCY 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 2 cut(s) 69, 560
FaiI YATR 9 cut(s) 67, 278, 338, 480, 493, 495, 548, 558, 604
FaqI GGGAC 2 cut(s) 89, 143
FatI CATG 2 cut(s) 65, 556
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 493
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 5 cut(s) 44, 47, 187, 190, 429
FokI GGATG 5 cut(s) 158, 254, 338, 448, 582
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 5 cut(s) 44, 47, 187, 190, 429
FspBI CTAG 3 cut(s) 134, 389, 662
GluI GCNGC 5 cut(s) 44, 47, 187, 190, 429
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 726
HapII CCGG 1 cut(s) 99
HgaI GACGC 2 cut(s) 13, 49
Hin1II CATG 2 cut(s) 69, 560
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HinfI GANTC 4 cut(s) 95, 392, 474, 530
HpaII CCGG 1 cut(s) 99
Hpy166II GTNNAC 3 cut(s) 154, 515, 748
Hpy188I TCNGA 3 cut(s) 435, 580, 592
Hpy188III TCNNGA 2 cut(s) 302, 389
Hpy8I GTNNAC 3 cut(s) 154, 515, 748
Hpy99I CGWCG 7 cut(s) 25, 28, 34, 37, 40, 43, 182
HpyAV CCTTC 2 cut(s) 203, 697
HpyCH4III ACNGT 1 cut(s) 519
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 286
HpyF3I CTNAG 2 cut(s) 440, 646
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 2 cut(s) 69, 560
KpnI GGTACC 1 cut(s) 118
LmnI GCTCC 4 cut(s) 14, 142, 497, 607
LpnPI CCDG 9 cut(s) 58, 112, 131, 266, 470, 633, 687, 708, 735
Lsp1109I GCAGC 4 cut(s) 55, 58, 173, 415
LweI GCATC 3 cut(s) 180, 355, 426
MaeI CTAG 3 cut(s) 134, 389, 662
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 487
MboII GAAGA 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 3 cut(s) 412, 656, 709
MlyI GAGTC 2 cut(s) 386, 483
MnlI CCTC 7 cut(s) 5, 185, 226, 229, 447, 470, 646
MroXI GAANNNNTTC 2 cut(s) 344, 412
MseI TTAA 2 cut(s) 309, 729
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 100, 723
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 723
MwoI GCNNNNNNNGC 1 cut(s) 286
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 493
NlaIII CATG 2 cut(s) 69, 560
NlaIV GGNNCC 4 cut(s) 16, 116, 458, 499
NmuCI GTSAC 1 cut(s) 487
PceI AGGCCT 1 cut(s) 726
PcsI WCGNNNNNNNCGW 4 cut(s) 26, 29, 32, 228
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 344, 412
PfeI GAWTC 2 cut(s) 95, 530
PkrI GCNGC 5 cut(s) 45, 48, 188, 191, 430
PleI GAGTC 2 cut(s) 386, 482
PpsI GAGTC 2 cut(s) 386, 482
PpuMI RGGWCCY 1 cut(s) 457
PsiI TTATAA 1 cut(s) 548
Psp5II RGGWCCY 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 721
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 4 cut(s) 16, 116, 458, 499
PspPI GGNCC 1 cut(s) 457
PspPPI RGGWCCY 1 cut(s) 457
RsaI GTAC 6 cut(s) 71, 116, 125, 155, 230, 700
RsaNI GTAC 6 cut(s) 70, 115, 124, 154, 229, 699
SalI GTCGAC 1 cut(s) 746
SaqAI TTAA 2 cut(s) 309, 729
SatI GCNGC 5 cut(s) 44, 47, 187, 190, 429
Sau96I GGNCC 1 cut(s) 457
SchI GAGTC 2 cut(s) 386, 483
ScrFI CCNGG 2 cut(s) 100, 723
SduI GDGCHC 1 cut(s) 502
SetI ASST 8 cut(s) 139, 159, 240, 277, 309, 539, 612, 647
SfaNI GCATC 3 cut(s) 180, 355, 426
SinI GGWCC 1 cut(s) 457
SmlI CTYRAG 2 cut(s) 461, 728
SmoI CTYRAG 2 cut(s) 461, 728
Sse9I AATT 3 cut(s) 412, 656, 709
SseBI AGGCCT 1 cut(s) 726
SsiI CCGC 8 cut(s) 82, 163, 169, 189, 198, 218, 354, 405
SspI AATATT 1 cut(s) 314
SspMI CTAG 3 cut(s) 134, 389, 662
StuI AGGCCT 1 cut(s) 726
StyD4I CCNGG 2 cut(s) 98, 721
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 519
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 108, 249, 747
TasI AATT 3 cut(s) 412, 656, 709
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 2 cut(s) 95, 530
Tru1I TTAA 2 cut(s) 309, 729
Tru9I TTAA 2 cut(s) 309, 729
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 4 cut(s) 43, 46, 186, 428
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 246, 353
TspRI CASTG 1 cut(s) 448
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 457
XbaI TCTAGA 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 2 cut(s) 344, 412
XspI CTAG 3 cut(s) 134, 389, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.