FvH4_4g21180

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012435
Physical Location & Seq
Reverse (-)
24296913 .. 24299636
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21180.t2

Sequence Viewer

Length: 747 bp
ATGACGATGGGAGGAGCCACGACGACGCAGCAGCAAGCCTATGGCGAGCCATGGTACTGGGACAACCGCTACACCAACGAATCCGGACCTTTTGATTGGTACCAGAAATACCAGTCTCTAGCTCCCATCATCAACCTCTACGTCCCCCGCCACCTCCGCCACCGCGTCCTCGTCGTCGGCTGCGGCAACTCCGCTTTTAGCGAGGGAATGGCGGACGATGGGTACGAGGACGTGGTTAGTATCGACATTTCGTCTGTGGTGATCCAAGCTATGCAGGACAAGCACTCGAACCGTCAGGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCGAAACTGCTTCCTTTGATGCTGTCATTGACAAAGGAACTCTGGACTCTCTTTTGTGTGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAATGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTGACATATGGAGCCCCGTTGTATCGTTTACGATTGCTGAAAGAATCAAGCTCATGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCAATGATAAATCAGAGCATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGAAGCTCCGCAGAGGCATTGCTAGGAAACAACCCTGATGTCCATTTTATTTACATTTGTACAAAGGATAATTCTTCAAAGCCAGGCTTTAAGCGTGAAGTATCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

249

Amino Acids

28.05

Weight (kDa)

5.14

Isoelectric Point (pI)

23.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 19 - 162 4.4e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 55 - 164 3.7e-15 Methyltransferase domain
Methyltransf_25 PF13649 56 - 156 2.9e-12 Methyltransferase domain
Methyltransf_11 PF08241 57 - 159 2.9e-15 Methyltransferase domain
Methyltransf_12 PF08242 57 - 157 3.8e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 741
AccII CGCG 1 cut(s) 165
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 8 cut(s) 67, 148, 157, 163, 183, 192, 212, 642
AclWI GGATC 1 cut(s) 256
AfaI GTAC 4 cut(s) 56, 101, 224, 694
AgsI TTSAA 2 cut(s) 625, 711
AhdI GACNNNNNGTC 1 cut(s) 250
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 5 cut(s) 122, 269, 531, 604, 639
AluI AGCT 5 cut(s) 122, 269, 531, 604, 639
Alw26I GTCTC 1 cut(s) 120
AlwI GGATC 1 cut(s) 256
Aor13HI TCCGGA 1 cut(s) 83
ApeKI GCWGC 4 cut(s) 28, 31, 180, 422
Asp700I GAANNNNTTC 2 cut(s) 338, 406
Asp718I GGTACC 1 cut(s) 99
AspS9I GGNCC 3 cut(s) 86, 298, 451
AsuHPI GGTGA 2 cut(s) 271, 493
AsuII TTCGAA 1 cut(s) 342
AvaII GGWCC 3 cut(s) 86, 298, 451
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 496
BbvI GCAGC 4 cut(s) 40, 43, 167, 409
BccI CCATC 3 cut(s) 134, 212, 626
BciT130I CCWGG 1 cut(s) 717
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 2 cut(s) 119, 656
BisI GCNGC 5 cut(s) 29, 32, 181, 184, 423
BlsI GCNGC 5 cut(s) 30, 33, 182, 185, 424
Bme1390I CCNGG 1 cut(s) 717
Bme18I GGWCC 3 cut(s) 86, 298, 451
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 3 cut(s) 86, 298, 451
BmiI GGNNCC 4 cut(s) 16, 101, 452, 493
BmrFI CCNGG 1 cut(s) 717
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 3 cut(s) 349, 420, 598
BmuI ACTGGG 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 62, 112
Bse3DI GCAATG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 3 cut(s) 325, 435, 589
BseMI GCAATG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 62, 112
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 4 cut(s) 40, 43, 167, 409
Bsh1236I CGCG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 74, 128
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 74, 128
BsmI GAATGC 1 cut(s) 340
Bsp119I TTCGAA 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 496
Bsp13I TCCGGA 1 cut(s) 83
Bsp1407I TGTACA 1 cut(s) 692
Bsp143I GATC 1 cut(s) 261
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 8 cut(s) 67, 148, 157, 163, 183, 192, 212, 642
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 16, 101, 452, 493
BspPI GGATC 1 cut(s) 256
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 650
BsrGI TGTACA 1 cut(s) 692
BsrI ACTGG 2 cut(s) 62, 112
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 692
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 36, 47
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 325, 435, 589
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 156, 280
BstNI CCWGG 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 715
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 4 cut(s) 40, 43, 167, 409
BstXI CCANNNNNNTGG 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 325, 435, 589
Cac8I GCNNGC 2 cut(s) 36, 47
Cfr13I GGNCC 3 cut(s) 86, 298, 451
CseI GACGC 2 cut(s) 34, 154
Csp6I GTAC 4 cut(s) 55, 100, 223, 693
CviAII CATG 3 cut(s) 51, 534, 551
CviQI GTAC 4 cut(s) 55, 100, 223, 693
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DriI GACNNNNNGTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 250
EciI GGCGGA 2 cut(s) 146, 227
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 86, 298, 451
EcoO109I RGGNCCY 2 cut(s) 298, 451
EcoRII CCWGG 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 3 cut(s) 54, 537, 554
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 2 cut(s) 74, 128
FatI CATG 3 cut(s) 50, 533, 550
FauI CCCGC 1 cut(s) 155
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 741
Fnu4HI GCNGC 5 cut(s) 29, 32, 181, 184, 423
FokI GGATG 3 cut(s) 332, 442, 576
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 5 cut(s) 29, 32, 181, 184, 423
FspBI CTAG 2 cut(s) 119, 656
GluI GCNGC 5 cut(s) 29, 32, 181, 184, 423
HapII CCGG 1 cut(s) 84
HgaI GACGC 2 cut(s) 34, 154
Hin1II CATG 3 cut(s) 54, 537, 554
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HinfI GANTC 4 cut(s) 80, 386, 468, 524
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 271, 493
Hpy166II GTNNAC 2 cut(s) 509, 742
Hpy188I TCNGA 2 cut(s) 429, 586
Hpy188III TCNNGA 3 cut(s) 84, 296, 383
Hpy8I GTNNAC 2 cut(s) 509, 742
Hpy99I CGWCG 4 cut(s) 25, 28, 176, 179
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 2 cut(s) 141, 231
HpyCH4V TGCA 1 cut(s) 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 280
HpySE526I ACGT 2 cut(s) 141, 231
Hsp92II CATG 3 cut(s) 54, 537, 554
Kpn2I TCCGGA 1 cut(s) 83
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 5 cut(s) 14, 127, 491, 601, 644
Lsp1109I GCAGC 4 cut(s) 40, 43, 167, 409
LweI GCATC 3 cut(s) 349, 420, 598
MaeI CTAG 2 cut(s) 119, 656
MaeII ACGT 2 cut(s) 141, 231
MaeIII GTNAC 1 cut(s) 481
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 1 cut(s) 699
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 2 cut(s) 406, 703
MlyI GAGTC 2 cut(s) 380, 477
MnlI CCTC 9 cut(s) 5, 146, 164, 179, 196, 220, 441, 464, 640
MroI TCCGGA 1 cut(s) 83
MroXI GAANNNNTTC 2 cut(s) 338, 406
MseI TTAA 2 cut(s) 303, 723
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 717
Mva1269I GAATGC 1 cut(s) 340
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 156, 280
NcoI CCATGG 1 cut(s) 50
NdeI CATATG 1 cut(s) 487
NdeII GATC 1 cut(s) 261
NlaIII CATG 3 cut(s) 54, 537, 554
NlaIV GGNNCC 4 cut(s) 16, 101, 452, 493
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 340
PdmI GAANNNNTTC 2 cut(s) 338, 406
PfeI GAWTC 2 cut(s) 80, 524
PkrI GCNGC 5 cut(s) 30, 33, 182, 185, 424
PleI GAGTC 2 cut(s) 380, 476
PpsI GAGTC 2 cut(s) 380, 476
PpuMI RGGWCCY 2 cut(s) 298, 451
PsiI TTATAA 1 cut(s) 542
Psp5II RGGWCCY 2 cut(s) 298, 451
Psp6I CCWGG 1 cut(s) 715
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 4 cut(s) 16, 101, 452, 493
PspPI GGNCC 3 cut(s) 86, 298, 451
PspPPI RGGWCCY 2 cut(s) 298, 451
RsaI GTAC 4 cut(s) 56, 101, 224, 694
RsaNI GTAC 4 cut(s) 55, 100, 223, 693
SalI GTCGAC 1 cut(s) 740
SaqAI TTAA 2 cut(s) 303, 723
SatI GCNGC 5 cut(s) 29, 32, 181, 184, 423
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 3 cut(s) 86, 298, 451
SchI GAGTC 2 cut(s) 380, 477
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 3 cut(s) 349, 420, 598
SfuI TTCGAA 1 cut(s) 342
SinI GGWCC 3 cut(s) 86, 298, 451
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 2 cut(s) 406, 703
SsiI CCGC 8 cut(s) 67, 148, 157, 163, 183, 192, 212, 642
SspI AATATT 1 cut(s) 308
SspMI CTAG 2 cut(s) 119, 656
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 2 cut(s) 144, 234
TaqI TCGA 4 cut(s) 243, 287, 342, 741
TasI AATT 2 cut(s) 406, 703
TatI WGTACW 1 cut(s) 692
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 2 cut(s) 80, 524
Tru1I TTAA 2 cut(s) 303, 723
Tru9I TTAA 2 cut(s) 303, 723
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 4 cut(s) 28, 31, 180, 422
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 347, 453
VpaK11BI GGWCC 3 cut(s) 86, 298, 451
XmiI GTMKAC 1 cut(s) 741
XmnI GAANNNNTTC 2 cut(s) 338, 406
XspI CTAG 2 cut(s) 119, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.