Rh4BG276000

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012435
Physical Location & Seq
Reverse (-)
45008310 .. 45011068
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG276000.1

Sequence Viewer

Length: 765 bp
ATGACGCTGGGAGGAGCCACGACGACGACGACGACGACGCAGCAGCAAGCCTACGGCGAGCCATGGTACTGGGACAACCGCTACGCCAACGAATCCGGGGCGTTCGATTGGTACCAGAAGTACCAATCTCTAGCTCCCATTATCAATCTCTACGTCCCCCGCCACAACCACCTCCGCCACCGCATCCTCGTCGTCGGCTGCGGCAACTCAGCATTTAGCGAAGGAATGGCGGACGATGGGTACGAGGAGGTGGTTAGTATCGACATTTCATCTGTGGTCATCCAAGCTATGCAGGAGAAGCACTCCAATCGTCAAGACCTTAAATATTTGGAAATGGATGTGAGAGATATGAATGCTTTCCAAACCGCTTCCTTTGATGCTGTTGTTGACAAAGGAACTCTAGACTCTCTTCTGTGCGGAAGTAATTCACAACAAAATGCTGCTCGGATGCTCAGTGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTATATTCTGGTCACATATGGAGCCCCGTTGTATCGTTTACGGTTGCTGAAAGAATCAAGCTCGTGGGTTATAAAACTCCATGTGATAGATAAACTTGTTTCCGATGATAAATCAGAACATCCAATATGGGAGCTGACAAATCCTGTTCCCTTGAATGATGATGGAAGCTCAGCAGAGGAATTGCTAGGAAACAACCCTGATGTCCATTTTATTTACATTTGTACGAAGGATAATTCTTCGAAGCCAGGCCTTAAGCGTGAAGTGTCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

254

Amino Acids

28.65

Weight (kDa)

5.16

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 23 - 168 3.7e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 61 - 170 5.9e-15 Methyltransferase domain
Methyltransf_25 PF13649 62 - 162 1.9e-12 Methyltransferase domain
Methyltransf_11 PF08241 63 - 165 2.2e-15 Methyltransferase domain
Methyltransf_12 PF08242 63 - 163 6.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 759
AciI CCGC 8 cut(s) 79, 160, 175, 181, 201, 230, 366, 417
AfaI GTAC 5 cut(s) 68, 113, 122, 242, 712
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 1 cut(s) 643
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 5 cut(s) 134, 287, 549, 622, 657
AluI AGCT 5 cut(s) 134, 287, 549, 622, 657
AoxI GGCC 1 cut(s) 736
ApeKI GCWGC 4 cut(s) 40, 43, 198, 440
Asp700I GAANNNNTTC 2 cut(s) 356, 424
Asp718I GGTACC 1 cut(s) 111
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 97
AsuII TTCGAA 1 cut(s) 728
AvaII GGWCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 1 cut(s) 550
BbvI GCAGC 4 cut(s) 52, 55, 185, 427
BccI CCATC 2 cut(s) 230, 644
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 172, 206, 290, 324
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 1 cut(s) 97
BfaI CTAG 3 cut(s) 131, 401, 674
BfrI CTTAAG 1 cut(s) 740
BisI GCNGC 5 cut(s) 41, 44, 199, 202, 441
BlpI GCTNAGC 1 cut(s) 658
BlsI GCNGC 5 cut(s) 42, 45, 200, 203, 442
Bme1390I CCNGG 2 cut(s) 97, 735
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 4 cut(s) 16, 113, 470, 511
BmrFI CCNGG 2 cut(s) 97, 735
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 3 cut(s) 192, 367, 438
BmuI ACTGGG 1 cut(s) 79
BplI GAGNNNNNCTC 2 cut(s) 287, 319
Bpu1102I GCTNAGC 1 cut(s) 658
Bpu14I TTCGAA 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 62, 96
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 2 cut(s) 62, 96
BseGI GGATG 5 cut(s) 183, 279, 343, 453, 607
BseMII CTCAG 3 cut(s) 222, 466, 672
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 2 cut(s) 27, 260
BseXI GCAGC 4 cut(s) 52, 55, 185, 427
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 738
BshNI GGYRCC 1 cut(s) 111
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 86, 140
BsmFI GGGAC 2 cut(s) 86, 140
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 738
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 514
Bsp1720I GCTNAGC 1 cut(s) 658
Bsp19I CCATGG 1 cut(s) 62
BspACI CCGC 8 cut(s) 79, 160, 175, 181, 201, 230, 366, 417
BspANI GGCC 1 cut(s) 738
BspCNI CTCAG 3 cut(s) 221, 465, 671
BspLI GGNNCC 4 cut(s) 16, 113, 470, 511
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 111
BspTI CTTAAG 1 cut(s) 740
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 62, 96
BssSI CACGAG 1 cut(s) 550
BssT1I CCWWGG 1 cut(s) 62
Bst2BI CACGAG 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 48, 59
BstDEI CTNAG 3 cut(s) 208, 452, 658
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 5 cut(s) 183, 279, 343, 453, 607
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 2 cut(s) 95, 733
BstV1I GCAGC 4 cut(s) 52, 55, 185, 427
BstXI CCANNNNNNTGG 1 cut(s) 69
BsuRI GGCC 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 5 cut(s) 183, 279, 343, 453, 607
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 2 cut(s) 48, 59
Cfr13I GGNCC 1 cut(s) 469
CseI GACGC 2 cut(s) 13, 46
Csp6I GTAC 5 cut(s) 67, 112, 121, 241, 711
CviAII CATG 2 cut(s) 63, 569
CviQI GTAC 5 cut(s) 67, 112, 121, 241, 711
DdeI CTNAG 3 cut(s) 208, 452, 658
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 2 cut(s) 164, 245
Eco130I CCWWGG 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 738
Eco24I GRGCYC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 2 cut(s) 66, 572
FaiI YATR 9 cut(s) 64, 290, 350, 492, 505, 507, 560, 570, 616
FaqI GGGAC 2 cut(s) 86, 140
FatI CATG 2 cut(s) 62, 568
FauI CCCGC 1 cut(s) 167
FauNDI CATATG 1 cut(s) 505
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 5 cut(s) 41, 44, 199, 202, 441
FokI GGATG 5 cut(s) 170, 266, 350, 460, 594
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 5 cut(s) 41, 44, 199, 202, 441
FspBI CTAG 3 cut(s) 131, 401, 674
GluI GCNGC 5 cut(s) 41, 44, 199, 202, 441
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 738
HapII CCGG 1 cut(s) 96
HgaI GACGC 2 cut(s) 13, 46
Hin1II CATG 2 cut(s) 66, 572
HincII GTYRAC 2 cut(s) 388, 760
HindII GTYRAC 2 cut(s) 388, 760
HinfI GANTC 4 cut(s) 92, 404, 486, 542
HpaII CCGG 1 cut(s) 96
Hpy166II GTNNAC 3 cut(s) 388, 527, 760
Hpy188I TCNGA 3 cut(s) 447, 592, 604
Hpy188III TCNNGA 2 cut(s) 314, 401
Hpy8I GTNNAC 3 cut(s) 388, 527, 760
Hpy99I CGWCG 8 cut(s) 25, 28, 31, 34, 37, 40, 194, 197
HpyAV CCTTC 2 cut(s) 215, 709
HpyCH4III ACNGT 1 cut(s) 531
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 1 cut(s) 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 3 cut(s) 208, 452, 658
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 2 cut(s) 66, 572
KpnI GGTACC 1 cut(s) 115
LmnI GCTCC 4 cut(s) 14, 139, 509, 619
LpnPI CCDG 9 cut(s) 55, 109, 128, 278, 482, 645, 699, 720, 747
Lsp1109I GCAGC 4 cut(s) 52, 55, 185, 427
LweI GCATC 3 cut(s) 192, 367, 438
MaeI CTAG 3 cut(s) 131, 401, 674
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 499
MboII GAAGA 2 cut(s) 401, 717
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 3 cut(s) 424, 668, 721
MlyI GAGTC 2 cut(s) 398, 495
MnlI CCTC 8 cut(s) 5, 182, 197, 238, 241, 459, 482, 658
MroXI GAANNNNTTC 2 cut(s) 356, 424
MseI TTAA 2 cut(s) 321, 741
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 97, 735
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 1 cut(s) 298
NciI CCSGG 1 cut(s) 97
NcoI CCATGG 1 cut(s) 62
NdeI CATATG 1 cut(s) 505
NlaIII CATG 2 cut(s) 66, 572
NlaIV GGNNCC 4 cut(s) 16, 113, 470, 511
NmuCI GTSAC 1 cut(s) 499
NspV TTCGAA 1 cut(s) 728
PceI AGGCCT 1 cut(s) 738
PcsI WCGNNNNNNNCGW 4 cut(s) 26, 29, 32, 240
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 2 cut(s) 356, 424
PfeI GAWTC 2 cut(s) 92, 542
PkrI GCNGC 5 cut(s) 42, 45, 200, 203, 442
PleI GAGTC 2 cut(s) 398, 494
PpsI GAGTC 2 cut(s) 398, 494
PpuMI RGGWCCY 1 cut(s) 469
PsiI TTATAA 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 4 cut(s) 16, 113, 470, 511
PspPI GGNCC 1 cut(s) 469
PspPPI RGGWCCY 1 cut(s) 469
RsaI GTAC 5 cut(s) 68, 113, 122, 242, 712
RsaNI GTAC 5 cut(s) 67, 112, 121, 241, 711
SalI GTCGAC 1 cut(s) 758
SaqAI TTAA 2 cut(s) 321, 741
SatI GCNGC 5 cut(s) 41, 44, 199, 202, 441
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 2 cut(s) 398, 495
ScrFI CCNGG 2 cut(s) 97, 735
SduI GDGCHC 1 cut(s) 514
SetI ASST 9 cut(s) 136, 156, 174, 252, 289, 321, 551, 624, 659
SfaNI GCATC 3 cut(s) 192, 367, 438
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 1 cut(s) 469
SmlI CTYRAG 2 cut(s) 473, 740
SmoI CTYRAG 2 cut(s) 473, 740
Sse9I AATT 3 cut(s) 424, 668, 721
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 8 cut(s) 79, 160, 175, 181, 201, 230, 366, 417
SspI AATATT 1 cut(s) 326
SspMI CTAG 3 cut(s) 131, 401, 674
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 2 cut(s) 95, 733
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 1 cut(s) 531
TaiI ACGT 1 cut(s) 156
TaqI TCGA 4 cut(s) 105, 261, 728, 759
TasI AATT 3 cut(s) 424, 668, 721
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 2 cut(s) 92, 542
Tru1I TTAA 2 cut(s) 321, 741
Tru9I TTAA 2 cut(s) 321, 741
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 4 cut(s) 40, 43, 198, 440
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 2 cut(s) 258, 365
TspRI CASTG 1 cut(s) 460
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 469
XbaI TCTAGA 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 759
XmnI GAANNNNTTC 2 cut(s) 356, 424
XspI CTAG 3 cut(s) 131, 401, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.