MD13G1144200.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012435
Physical Location & Seq
Forward (+)
11211917 .. 11214140
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1144200.v1.1.491

Sequence Viewer

Length: 714 bp
ATGACGATGGGAACGACGACGACGACGCAGGCGTACGGCGAGCCGTGGTACTGGGACAACCGCTACGCCAACGAATCAGGGTCGTTCGATTGGTACCAAAAGTACCAATCTCTGGCTCCGCTGATCAATCTCTATGTCCCTCGCCATCCCAACCAACGCAACCGCATCCTTGTCGTCGGCTGCGGCAACTCAGCGTTTAGCGAGGGGATGGCTGATGATGGGTACGAGGAGGTGGTTAGTATTGACATTTCATCTGTGGTCATCCAAGCTATGCAGACCAAGTACTCGAATCGTCCGCACCTCAAATATTTGCAAATGGATGTTCGAGATATGAGTGCTTTCGAAACTGCTTCCTTTGATGCAGTTGTTGACAAAGGAACTCTAGACTCTCTTTTGTGCGGAAGTAATTCGCGGCAAAATGCTACCGAGATGCTTGAGGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTACGTTCTGATCACATATGGTGCCCCGTTGTATCGTTTGCGTTTGTTGAGAGAGTCATGCTCGTGGATGATAAAACTCCATGTCATAGAGAAACTTTCCTGCGAAGATAAATCAGAACCTCCAATTTGGGATCTGACCAATCCTGTTCCTCTAAATGATGATGGAAGCTCAACGGAGGAATTGCTGGGAAACAACCCTGATGTCCACTATATTTACGTTTGTACGAAGGATGATTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

238

Amino Acids

26.99

Weight (kDa)

4.74

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 17 - 162 5e-08 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 55 - 164 2.5e-12 Methyltransferase domain
Methyltransf_25 PF13649 56 - 156 5.1e-13 Methyltransferase domain
Methyltransf_11 PF08241 57 - 159 7.6e-16 Methyltransferase domain
Methyltransf_12 PF08242 57 - 157 1.2e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 93, 491
AccB7I CCANNNNNTGG 1 cut(s) 112
AccI GTMKAC 1 cut(s) 471
AccII CGCG 1 cut(s) 412
AciI CCGC 7 cut(s) 61, 119, 163, 183, 296, 399, 412
AclWI GGATC 1 cut(s) 609
AfaI GTAC 7 cut(s) 35, 50, 95, 104, 224, 284, 694
AfiI CCNNNNNNNGG 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
AluBI AGCT 2 cut(s) 269, 639
AluI AGCT 2 cut(s) 269, 639
AlwI GGATC 1 cut(s) 609
ApeKI GCWGC 1 cut(s) 180
Asp700I GAANNNNTTC 1 cut(s) 406
Asp718I GGTACC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 451
AsuII TTCGAA 1 cut(s) 342
AvaII GGWCC 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 496
BanI GGYRCC 2 cut(s) 93, 491
BauI CACGAG 1 cut(s) 532
BbvI GCAGC 1 cut(s) 167
BccI CCATC 4 cut(s) 153, 202, 212, 626
BceAI ACGGC 2 cut(s) 28, 52
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BclI TGATCA 2 cut(s) 123, 480
BfaI CTAG 1 cut(s) 383
BisI GCNGC 3 cut(s) 181, 184, 413
BlsI GCNGC 3 cut(s) 182, 185, 414
BmcAI AGTACT 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 451
BmiI GGNNCC 4 cut(s) 95, 117, 452, 493
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 174, 349, 420
BmuI ACTGGG 1 cut(s) 61
BplI GAGNNNNNCTC 2 cut(s) 515, 547
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 440, 455
BsaJI CCNNGG 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 7 cut(s) 145, 165, 213, 261, 325, 543, 706
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 1 cut(s) 167
BseYI CCCAGC 1 cut(s) 655
Bsh1236I CGCG 1 cut(s) 412
BshNI GGYRCC 2 cut(s) 93, 491
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 68, 122
BslI CCNNNNNNNGG 1 cut(s) 112
BsmFI GGGAC 2 cut(s) 68, 122
Bsp119I TTCGAA 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 3 cut(s) 123, 480, 601
BspACI CCGC 7 cut(s) 61, 119, 163, 183, 296, 399, 412
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 4 cut(s) 95, 117, 452, 493
BspPI GGATC 1 cut(s) 609
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 2 cut(s) 93, 491
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 44
BssMI GATC 3 cut(s) 123, 480, 601
BssSI CACGAG 1 cut(s) 532
Bst2BI CACGAG 1 cut(s) 532
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 30, 41
BstDEI CTNAG 1 cut(s) 190
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 7 cut(s) 145, 165, 213, 261, 325, 543, 706
BstFNI CGCG 1 cut(s) 412
BstKTI GATC 3 cut(s) 126, 483, 604
BstMBI GATC 3 cut(s) 123, 480, 601
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 1 cut(s) 167
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 7 cut(s) 145, 165, 213, 261, 325, 543, 706
Cac8I GCNNGC 2 cut(s) 30, 41
Cfr13I GGNCC 1 cut(s) 451
CseI GACGC 1 cut(s) 34
Csp6I GTAC 7 cut(s) 34, 49, 94, 103, 223, 283, 693
CviAII CATG 2 cut(s) 528, 551
CviJI RGCY 6 cut(s) 43, 116, 180, 212, 269, 639
CviKI_1 RGCY 6 cut(s) 43, 116, 180, 212, 269, 639
CviQI GTAC 7 cut(s) 34, 49, 94, 103, 223, 283, 693
DdeI CTNAG 1 cut(s) 190
DpnI GATC 3 cut(s) 125, 482, 603
DpnII GATC 3 cut(s) 123, 480, 601
Eco47I GGWCC 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 451
FaeI CATG 2 cut(s) 531, 554
FaiI YATR 9 cut(s) 135, 272, 332, 487, 489, 529, 552, 557, 681
FaqI GGGAC 2 cut(s) 68, 122
FatI CATG 2 cut(s) 527, 550
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 2 cut(s) 123, 480
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 3 cut(s) 181, 184, 413
FokI GGATG 6 cut(s) 132, 152, 220, 248, 332, 550
Fsp4HI GCNGC 3 cut(s) 181, 184, 413
FspBI CTAG 1 cut(s) 383
GluI GCNGC 3 cut(s) 181, 184, 413
GsaI CCCAGC 1 cut(s) 659
HgaI GACGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 531, 554
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 6 cut(s) 74, 289, 386, 468, 524, 704
Hpy166II GTNNAC 3 cut(s) 370, 472, 676
Hpy188I TCNGA 3 cut(s) 480, 586, 606
Hpy188III TCNNGA 2 cut(s) 326, 383
Hpy8I GTNNAC 3 cut(s) 370, 472, 676
Hpy99I CGWCG 5 cut(s) 19, 22, 25, 28, 179
HpyAV CCTTC 1 cut(s) 691
HpyCH4IV ACGT 2 cut(s) 474, 687
HpyCH4V TGCA 3 cut(s) 274, 313, 362
HpyF3I CTNAG 1 cut(s) 190
HpySE526I ACGT 2 cut(s) 474, 687
Hsp92II CATG 2 cut(s) 531, 554
KpnI GGTACC 1 cut(s) 97
Ksp22I TGATCA 2 cut(s) 123, 480
Kzo9I GATC 3 cut(s) 123, 480, 601
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 8 cut(s) 14, 37, 63, 98, 583, 627, 641, 681
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 3 cut(s) 174, 349, 420
MaeI CTAG 1 cut(s) 383
MaeII ACGT 2 cut(s) 474, 687
MalI GATC 3 cut(s) 125, 482, 603
MboI GATC 3 cut(s) 123, 480, 601
MboII GAAGA 1 cut(s) 587
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 3 cut(s) 406, 594, 650
MlyI GAGTC 3 cut(s) 380, 477, 533
MroXI GAANNNNTTC 1 cut(s) 406
MslI CAYNNNNRTG 1 cut(s) 532
MspA1I CMGCKG 1 cut(s) 121
MvnI CGCG 1 cut(s) 412
NdeI CATATG 1 cut(s) 487
NdeII GATC 3 cut(s) 123, 480, 601
NlaIII CATG 2 cut(s) 531, 554
NlaIV GGNNCC 4 cut(s) 95, 117, 452, 493
NspV TTCGAA 1 cut(s) 342
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 20, 29
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 3 cut(s) 74, 289, 704
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 3 cut(s) 182, 185, 414
PleI GAGTC 3 cut(s) 380, 476, 532
PpsI GAGTC 3 cut(s) 380, 476, 532
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
PspFI CCCAGC 1 cut(s) 655
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 95, 117, 452, 493
PspPI GGNCC 1 cut(s) 451
PspPPI RGGWCCY 1 cut(s) 451
PsuI RGATCY 1 cut(s) 601
RsaI GTAC 7 cut(s) 35, 50, 95, 104, 224, 284, 694
RsaNI GTAC 7 cut(s) 34, 49, 94, 103, 223, 283, 693
RseI CAYNNNNRTG 1 cut(s) 532
SatI GCNGC 3 cut(s) 181, 184, 413
Sau3AI GATC 3 cut(s) 123, 480, 601
Sau96I GGNCC 1 cut(s) 451
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 3 cut(s) 380, 477, 533
SduI GDGCHC 1 cut(s) 496
SetI ASST 7 cut(s) 234, 271, 303, 477, 592, 641, 690
SfaNI GCATC 3 cut(s) 174, 349, 420
SfuI TTCGAA 1 cut(s) 342
SinI GGWCC 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 532
SmlI CTYRAG 2 cut(s) 434, 455
SmoI CTYRAG 2 cut(s) 434, 455
Sse9I AATT 3 cut(s) 406, 594, 650
SsiI CCGC 7 cut(s) 61, 119, 163, 183, 296, 399, 412
SspI AATATT 1 cut(s) 308
SspMI CTAG 1 cut(s) 383
TaiI ACGT 2 cut(s) 477, 690
TaqI TCGA 4 cut(s) 87, 287, 325, 342
TasI AATT 3 cut(s) 406, 594, 650
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 186, 415
TfiI GAWTC 3 cut(s) 74, 289, 704
TseI GCWGC 1 cut(s) 180
TspDTI ATGAA 1 cut(s) 240
TspGWI ACGGA 1 cut(s) 659
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 451
XbaI TCTAGA 1 cut(s) 382
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 1 cut(s) 383
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.